Bhi01G000843 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi01G000843
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Rapid ALkalinization Factor
Location	chr1 : 22690493 .. 22691978 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTATTTTAACAATGAGTAGAAATTTTATTTCAAATGTTTTATTGAGTGAATAAAATGGGAAAACACTATACATACATATGAAATTGAAAAACAATAATAACGTTTTTCATAAACAAAAATTAAATAATAAATTGGTAATGTTTTGACTCACCTACCCCTTTAATTATTTTTTGCATCACAGATGTTTCCCAAAGTTTTCAAAAAGGTTTGGACAAACTTCACCATGAAAAGCAAATACTCAAAAGCAATTGGTTAGGTTTTTGTTTTTTTAAAAGAAATTTGGTCCCTTTAATCTCACTAATGCTTCTCCCACTAATGCATGTTTCTCTATTCTTTTTCCCTCCATCACAAAAATTGAAGGAAAAAAAATGAAAAAAGAAATTAGATTTTATGCAATAATATCTATAAAGTTTCTCCCCATTAGTAAAGAAAGAACTAAAAGAAATGAGGCAAAAATTGTCTATCAAAAGTCAGAACAACAACATGAGAAGAAGAATCTCAAGCAAGCCAATTTGTTTACAATCTTGCTTTCTTCTCCTCTTTCTCTTTCTCCTCCTACTTCAAGATCTTTGTCTCGTTCGAGCTTTGTCGAAGCATTCTTGCAATGGCTCCATAGCCGAGTGTGGTAGCGAGGAGGAGATGCTGATGGAATCGGAGATAAGTCGAAGGTTTCTCGAACAACATAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGTGATGGCAGTGGTGGCGGTCAGCCTTACACCAAAAGCGGAAGCTGCGTGCCACCACCGACTAATCCTTACACTCGAGGCTGCTCAAAGATATATCGTTGTAGGTCTGATGATTGATGGCTTGAACAAACCATAGTATGCTTTGTAGAATCTCTTTTTTTTCTTGTTCTTGTTGTGTTCTTGTTGGTTGATGAAGGGATTTAATTATGAATTAATGAAGCTTGAAATGGGATTTTTCTTTTTCATTGTGATCTCATTTTTGTTTTTGGCAAATAAGAGAGATGAGAAGTGTTAGAAATGTTTGGTTGGACATCATGTTGGCCAGGATGTAAAGTGTGAAAACCAACCTCATACCTAATCTTATCTTTTGTCTTGCATAGGAACTTGTCGGGATTTGTTCATTCTTAATGTACTTTTCATTTTAAAGAAGTATTTTTTTATTAAAGAACGTACGTAATCTATCCATATTCATCTATATGATCAAGTTACTTGATTCATACAACGATGTTTTATTGTTTTTTATTTCCCCTTATTTTAAATGCCTCGATCTTTCGACTTACGTTCAGTCTATGTTGAGAAAATTTGTCATTTCAATAAAACTATAGGTAATAATCAATATAGAAGTTGTGCATAGAAAGAACTCAATGTAAGTCATGCAAGGTAAATTTGTTTCTTATTTAAATCTTAATGTACTTTTCTCTCTCTTAAAATAAAAGAAATTACATCGTTTTAATCCGAAAGTATGAACACTATAC

mRNA sequence

TTTTATTTTAACAATGAGTAGAAATTTTATTTCAAATGTTTTATTGAGTGAATAAAATGGGAAAACACTATACATACATATGAAATTGAAAAACAATAATAACGTTTTTCATAAACAAAAATTAAATAATAAATTGGTAATGTTTTGACTCACCTACCCCTTTAATTATTTTTTGCATCACAGATGTTTCCCAAAGTTTTCAAAAAGGTTTGGACAAACTTCACCATGAAAAGCAAATACTCAAAAGCAATTGGTTAGGTTTTTGTTTTTTTAAAAGAAATTTGGTCCCTTTAATCTCACTAATGCTTCTCCCACTAATGCATGTTTCTCTATTCTTTTTCCCTCCATCACAAAAATTGAAGGAAAAAAAATGAAAAAAGAAATTAGATTTTATGCAATAATATCTATAAAGTTTCTCCCCATTAGTAAAGAAAGAACTAAAAGAAATGAGGCAAAAATTGTCTATCAAAAGTCAGAACAACAACATGAGAAGAAGAATCTCAAGCAAGCCAATTTGTTTACAATCTTGCTTTCTTCTCCTCTTTCTCTTTCTCCTCCTACTTCAAGATCTTTGTCTCGTTCGAGCTTTGTCGAAGCATTCTTGCAATGGCTCCATAGCCGAGTGTGGTAGCGAGGAGGAGATGCTGATGGAATCGGAGATAAGTCGAAGGTTTCTCGAACAACATAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGTGATGGCAGTGGTGGCGGTCAGCCTTACACCAAAAGCGGAAGCTGCGTGCCACCACCGACTAATCCTTACACTCGAGGCTGCTCAAAGATATATCGTTGTAGGTCTGATGATTGATGGCTTGAACAAACCATAGTATGCTTTGTAGAATCTCTTTTTTTTCTTGTTCTTGTTGTGTTCTTGTTGGTTGATGAAGGGATTTAATTATGAATTAATGAAGCTTGAAATGGGATTTTTCTTTTTCATTGTGATCTCATTTTTGTTTTTGGCAAATAAGAGAGATGAGAAGTGTTAGAAATGTTTGGTTGGACATCATGTTGGCCAGGATGTAAAGTGTGAAAACCAACCTCATACCTAATCTTATCTTTTGTCTTGCATAGGAACTTGTCGGGATTTGTTCATTCTTAATGTACTTTTCATTTTAAAGAAGTATTTTTTTATTAAAGAACGTACGTAATCTATCCATATTCATCTATATGATCAAGTTACTTGATTCATACAACGATGTTTTATTGTTTTTTATTTCCCCTTATTTTAAATGCCTCGATCTTTCGACTTACGTTCAGTCTATGTTGAGAAAATTTGTCATTTCAATAAAACTATAGGTAATAATCAATATAGAAGTTGTGCATAGAAAGAACTCAATGTAAGTCATGCAAGGTAAATTTGTTTCTTATTTAAATCTTAATGTACTTTTCTCTCTCTTAAAATAAAAGAAATTACATCGTTTTAATCCGAAAGTATGAACACTATAC

Coding sequence (CDS)

ATGAGGCAAAAATTGTCTATCAAAAGTCAGAACAACAACATGAGAAGAAGAATCTCAAGCAAGCCAATTTGTTTACAATCTTGCTTTCTTCTCCTCTTTCTCTTTCTCCTCCTACTTCAAGATCTTTGTCTCGTTCGAGCTTTGTCGAAGCATTCTTGCAATGGCTCCATAGCCGAGTGTGGTAGCGAGGAGGAGATGCTGATGGAATCGGAGATAAGTCGAAGGTTTCTCGAACAACATAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGTGATGGCAGTGGTGGCGGTCAGCCTTACACCAAAAGCGGAAGCTGCGTGCCACCACCGACTAATCCTTACACTCGAGGCTGCTCAAAGATATATCGTTGTAGGTCTGATGATTGA

Protein sequence

MRQKLSIKSQNNNMRRRISSKPICLQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSDD

Homology

[Close]

BLAST of Bhi01G000843 vs. TAIR10
Match: AT4G14010.1 (ralf-like 32)

HSP 1 Score: 89.4 bits (220), Expect = 1.9e-18
Identity = 53/117 (45.30%), Postives = 69/117 (58.97%), Query Frame = 0


Query: 21  KPICLQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGS-----EEEMLMESEISRR 80
           KP  + S   + FL LLL       +A S   CNGS+AEC S     E  ++MES  S+R
Sbjct: 4   KPSRIFSTITIFFLCLLLAH--VTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQR 63

Query: 81  FLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSD 133
             E+    +S GAL+++ PACDG   G+ Y  S  C+PPP+NPY+RGCSK YRC  D
Sbjct: 64  LTEEQAHKLSYGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of Bhi01G000843 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 67.4 bits (163), Expect = 7.6e-12
Identity = 39/79 (49.37%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 53  CNGSIAEC-GSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSC 112
           C GSIAEC   EEEM  +S+ISRR L Q KKYIS GA++++   C   G      + G+ 
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILAQ-KKYISYGAMRRNSVPCSRRGASYYNCQRGA- 104

Query: 113 VPPPTNPYTRGCSKIYRCR 131
                NPY+RGCS I RCR
Sbjct: 105 ---QANPYSRGCSTITRCR 118

[Close]

BLAST of Bhi01G000843 vs. TAIR10
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 65.9 bits (159), Expect = 2.2e-11
Identity = 38/79 (48.10%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 53  CNGSIAECGSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCV 112
           C+GSIAEC   EE  M+SEI+RR L    KYIS  +LK++   C   G      ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILAT-TKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 113 PPPTNPYTRGCSKIYRCRS 132
               NPY+RGCSKI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of Bhi01G000843 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 63.5 bits (153), Expect = 1.1e-10
Identity = 46/119 (38.66%), Postives = 63/119 (52.94%), Query Frame = 0


Query: 16  RRISSKPICLQSCFLLL-FLFLLLLQDLCLVRALSKHSCNGSIAECG---SEEEMLMESE 75
           R +S+KP+ +    L + FLF  +           +  CNG+IAEC    +EEE  M+SE
Sbjct: 2   RGLSTKPVAIIIAILTVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSE 61

Query: 76  ISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCR 131
           I+RR L    KYIS GAL+++   C   G      + G+      NPY+RGCS I RCR
Sbjct: 62  INRRILAT-TKYISYGALRRNTVPCSRRGASYYNCRRGA----QANPYSRGCSAITRCR 115

[Close]

BLAST of Bhi01G000843 vs. TAIR10
Match: AT3G16570.1 (rapid alkalinization factor 23)

HSP 1 Score: 53.9 bits (128), Expect = 8.7e-08
Identity = 37/95 (38.95%), Postives = 48/95 (50.53%), Query Frame = 0


Query: 53  CNGSIAEC---------------GSE--EEMLMESEISRRFLEQHKKYISIGALKKDHPA 112
           C G+IAEC               G E  EE  M+SEI+RR L   ++YIS GAL+++   
Sbjct: 47  CRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILAT-RRYISYGALRRNTIP 106

Query: 113 CDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCR 131
           C   G      + G+      NPY+RGCS I RCR
Sbjct: 107 CSRRGASYYNCRRGA----QANPYSRGCSAITRCR 136

[Close]

BLAST of Bhi01G000843 vs. Swiss-Prot
Match: sp|O23262|RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.4e-17
Identity = 53/117 (45.30%), Postives = 69/117 (58.97%), Query Frame = 0


Query: 21  KPICLQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGS-----EEEMLMESEISRR 80
           KP  + S   + FL LLL       +A S   CNGS+AEC S     E  ++MES  S+R
Sbjct: 4   KPSRIFSTITIFFLCLLLAH--VTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQR 63

Query: 81  FLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSD 133
             E+    +S GAL+++ PACDG   G+ Y  S  C+PPP+NPY+RGCSK YRC  D
Sbjct: 64  LTEEQAHKLSYGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of Bhi01G000843 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-10
Identity = 39/79 (49.37%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 53  CNGSIAEC-GSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSC 112
           C GSIAEC   EEEM  +S+ISRR L Q KKYIS GA++++   C   G      + G+ 
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILAQ-KKYISYGAMRRNSVPCSRRGASYYNCQRGA- 104

Query: 113 VPPPTNPYTRGCSKIYRCR 131
                NPY+RGCS I RCR
Sbjct: 105 ---QANPYSRGCSTITRCR 118

[Close]

BLAST of Bhi01G000843 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.0e-10
Identity = 41/86 (47.67%), Postives = 48/86 (55.81%), Query Frame = 0


Query: 47  ALSKHSCNGSIAEC-GSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPY 106
           A S   C GSI EC   EEE  ++SE +RR L   KKYIS GAL+K+   C   G     
Sbjct: 35  ARSGGGCKGSIGECIAEEEEFELDSESNRRILAT-KKYISYGALQKNSVPCSRRGASYYN 94

Query: 107 TKSGSCVPPPTNPYTRGCSKIYRCRS 132
            K G+      NPY+RGCS I RCRS
Sbjct: 95  CKPGA----QANPYSRGCSAITRCRS 115

[Close]

BLAST of Bhi01G000843 vs. Swiss-Prot
Match: sp|Q9SRY3|RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.0e-10
Identity = 38/79 (48.10%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 53  CNGSIAECGSEEEMLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCV 112
           C+GSIAEC   EE  M+SEI+RR L    KYIS  +LK++   C   G      ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILAT-TKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 113 PPPTNPYTRGCSKIYRCRS 132
               NPY+RGCSKI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of Bhi01G000843 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-09
Identity = 46/119 (38.66%), Postives = 63/119 (52.94%), Query Frame = 0


Query: 16  RRISSKPICLQSCFLLL-FLFLLLLQDLCLVRALSKHSCNGSIAECG---SEEEMLMESE 75
           R +S+KP+ +    L + FLF  +           +  CNG+IAEC    +EEE  M+SE
Sbjct: 2   RGLSTKPVAIIIAILTVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSE 61

Query: 76  ISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCR 131
           I+RR L    KYIS GAL+++   C   G      + G+      NPY+RGCS I RCR
Sbjct: 62  INRRILAT-TKYISYGALRRNTVPCSRRGASYYNCRRGA----QANPYSRGCSAITRCR 115

[Close]

BLAST of Bhi01G000843 vs. TrEMBL
Match: tr|A0A0A0L9A8|A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 3.0e-47
Identity = 97/128 (75.78%), Postives = 106/128 (82.81%), Query Frame = 0


Query: 7   IKSQNNNMRRRISSKPICLQSCFL-LLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEE 66
           ++SQNNN R+RISSKP+ LQ+CFL           DLCLVRA S HSCNGSIA C +EEE
Sbjct: 1   MESQNNNTRKRISSKPMSLQTCFLXXXXXXXXXXXDLCLVRASSTHSCNGSIAACANEEE 60

Query: 67  MLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSK 126
           MLMESEI+RRFLEQ KKYISIGALKKDHPACDG+ GGQPYT+SGSC PPP NPY RGCSK
Sbjct: 61  MLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSK 120

Query: 127 IYRCRSDD 134
           IYRCRSDD
Sbjct: 121 IYRCRSDD 128

[Close]

BLAST of Bhi01G000843 vs. TrEMBL
Match: tr|A0A0A0L624|A0A0A0L624_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-28
Identity = 72/99 (72.73%), Postives = 76/99 (76.77%), Query Frame = 0


Query: 25  LQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQHKK-Y 84
           LQ+CFLLL     LLQDLCLVR  S HSCNGSIAEC +EEEMLMESEI+RRFL Q KK Y
Sbjct: 3   LQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKNY 62

Query: 85  ISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRG 123
           IS   LKKD PACDG+  GQPYTKSGSCVP   NPY  G
Sbjct: 63  ISYPTLKKDRPACDGA-SGQPYTKSGSCVPSQANPYNPG 100

[Close]

BLAST of Bhi01G000843 vs. TrEMBL
Match: tr|A0A1S3TB48|A0A1S3TB48_VIGRR (protein RALF-like 32 OS=Vigna radiata var. radiata OX=3916 GN=LOC106753652 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.4e-28
Identity = 68/118 (57.63%), Postives = 86/118 (72.88%), Query Frame = 0


Query: 18  ISSKP-ICLQSCFLLLFLFLLLLQDLCLVRALSKH--SCNGSIAECGSEEEMLMESEISR 77
           ++SKP   ++ CF  L  F  +    C V +L+ H  +CNGSIAEC  E+E+LMESEISR
Sbjct: 1   MASKPSTIIRLCFFYLMFFGFMRLSSCTVLSLTPHVSTCNGSIAECNQEDELLMESEISR 60

Query: 78  RFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSD 133
           RFLEQ KKYIS GAL++D P C+G G G+ Y+ SG C+PPP+NP+ RGCSK YRCRSD
Sbjct: 61  RFLEQ-KKYISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRGCSKYYRCRSD 117

[Close]

BLAST of Bhi01G000843 vs. TrEMBL
Match: tr|A0A0S3R1D3|A0A0S3R1D3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G214200 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.2e-28
Identity = 67/118 (56.78%), Postives = 86/118 (72.88%), Query Frame = 0


Query: 18  ISSKP-ICLQSCFLLLFLFLLLLQDLCLVRALSKH--SCNGSIAECGSEEEMLMESEISR 77
           ++SKP   ++ CF+ L  F  +    C V +L+ H  +CNGSIAEC  E+E+LMESE SR
Sbjct: 1   MASKPSTIVRLCFIYLIFFSFMRLSSCTVLSLTSHVSTCNGSIAECNQEDELLMESETSR 60

Query: 78  RFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSD 133
           RFLEQ KKYIS GAL++D P C+G G G+ Y+ SG C+PPP+NP+ RGCSK YRCRSD
Sbjct: 61  RFLEQ-KKYISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRGCSKYYRCRSD 117

[Close]

BLAST of Bhi01G000843 vs. TrEMBL
Match: tr|C6T1A5|C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100500295 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.2e-28
Identity = 63/107 (58.88%), Postives = 77/107 (71.96%), Query Frame = 0


Query: 28  CFLLLFLFLLLLQDLCLVRALSKH--SCNGSIAECGSEEEMLMESEISRRFLEQHKKYIS 87
           CF    LF  +    C V +L  H  +CNGSIAEC  E+E+LMESEISRRFLEQ + YIS
Sbjct: 12  CFCYFMLFSFMHFSSCTVFSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYIS 71

Query: 88  IGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSD 133
            GAL++D P C+G G G+ Y+K+G C+PPP+NP  RGCSK YRCRSD
Sbjct: 72  NGALQRDKPVCNGGGSGEAYSKTGGCLPPPSNPQNRGCSKYYRCRSD 118

[Close]

BLAST of Bhi01G000843 vs. NCBI nr
Match: XP_004147646.2 (PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical protein Csa_3G171840 [Cucumis sativus])

HSP 1 Score: 196.8 bits (499), Expect = 4.6e-47
Identity = 97/128 (75.78%), Postives = 106/128 (82.81%), Query Frame = 0


Query: 7   IKSQNNNMRRRISSKPICLQSCFL-LLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEE 66
           ++SQNNN R+RISSKP+ LQ+CFL           DLCLVRA S HSCNGSIA C +EEE
Sbjct: 1   MESQNNNTRKRISSKPMSLQTCFLXXXXXXXXXXXDLCLVRASSTHSCNGSIAACANEEE 60

Query: 67  MLMESEISRRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSK 126
           MLMESEI+RRFLEQ KKYISIGALKKDHPACDG+ GGQPYT+SGSC PPP NPY RGCSK
Sbjct: 61  MLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSK 120

Query: 127 IYRCRSDD 134
           IYRCRSDD
Sbjct: 121 IYRCRSDD 128

[Close]

BLAST of Bhi01G000843 vs. NCBI nr
Match: XP_023528073.1 (protein RALF-like 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 194.1 bits (492), Expect = 3.0e-46
Identity = 94/108 (87.04%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 26  QSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQHKKYIS 85
           +SCFL   LFLLL+QDLCLVRA SKHSCNGSIAECG EEE LMESEISRRFLEQ KKYIS
Sbjct: 7   KSCFL---LFLLLVQDLCLVRASSKHSCNGSIAECGGEEETLMESEISRRFLEQQKKYIS 66

Query: 86  IGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSDD 134
           IGALKKDHPACDG GGGQPYT+SGSC PPP NPY RGCSKIYRCRSDD
Sbjct: 67  IGALKKDHPACDGGGGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of Bhi01G000843 vs. NCBI nr
Match: XP_022956250.1 (protein RALF-like 32 [Cucurbita moschata])

HSP 1 Score: 191.8 bits (486), Expect = 1.5e-45
Identity = 93/108 (86.11%), Postives = 96/108 (88.89%), Query Frame = 0


Query: 26  QSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQHKKYIS 85
           +SCFL   LFLLL+QDLCLVRA SKHSCNGSIAECG E+E LMESEISRRFL Q KKYIS
Sbjct: 7   KSCFL---LFLLLVQDLCLVRASSKHSCNGSIAECGGEDETLMESEISRRFLAQQKKYIS 66

Query: 86  IGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSDD 134
           IGALKKDHPACDG GGGQPYTKSGSC PPP NPY RGCSKIYRCRSDD
Sbjct: 67  IGALKKDHPACDGGGGGQPYTKSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of Bhi01G000843 vs. NCBI nr
Match: XP_022138047.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 182.2 bits (461), Expect = 1.2e-42
Identity = 95/120 (79.17%), Postives = 100/120 (83.33%), Query Frame = 0


Query: 14  MRRRISSKPICLQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEIS 73
           MRRR+SSKP   Q  FLLL   L LLQ LCL+RA     CNGSIAEC +E+EMLMESEIS
Sbjct: 1   MRRRMSSKPKPSQPFFLLL---LFLLQALCLLRASFASPCNGSIAECNTEKEMLMESEIS 60

Query: 74  RRFLEQHKKYISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRGCSKIYRCRSDD 133
           RRFLEQ KKYISIGALKKD PACDGSG GQPYTKSGSCVPPP+NPY RGCSKIYRCRSDD
Sbjct: 61  RRFLEQQKKYISIGALKKDRPACDGSGRGQPYTKSGSCVPPPSNPYDRGCSKIYRCRSDD 117

[Close]

BLAST of Bhi01G000843 vs. NCBI nr
Match: KGN57228.1 (hypothetical protein Csa_3G171850 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 1.6e-28
Identity = 72/99 (72.73%), Postives = 76/99 (76.77%), Query Frame = 0


Query: 25  LQSCFLLLFLFLLLLQDLCLVRALSKHSCNGSIAECGSEEEMLMESEISRRFLEQHKK-Y 84
           LQ+CFLLL     LLQDLCLVR  S HSCNGSIAEC +EEEMLMESEI+RRFL Q KK Y
Sbjct: 3   LQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKNY 62

Query: 85  ISIGALKKDHPACDGSGGGQPYTKSGSCVPPPTNPYTRG 123
           IS   LKKD PACDG+  GQPYTKSGSCVP   NPY  G
Sbjct: 63  ISYPTLKKDRPACDGA-SGQPYTKSGSCVPSQANPYNPG 100

The following BLAST results are available for this feature:

BLAST of Bhi01G000843 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14010.1	1.9e-18	45.30	ralf-like 32	[more]
AT3G05490.1	7.6e-12	49.37	ralf-like 22	[more]
AT1G02900.1	2.2e-11	48.10	rapid alkalinization factor 1	[more]
AT4G15800.1	1.1e-10	38.66	ralf-like 33	[more]
AT3G16570.1	8.7e-08	38.95	rapid alkalinization factor 23	[more]

BLAST of Bhi01G000843 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O23262\|RLF32_ARATH	3.4e-17	45.30	Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1	[more]
sp\|Q9MA62\|RLF22_ARATH	1.4e-10	49.37	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
sp\|Q945T0\|RALF_TOBAC	4.0e-10	47.67	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
sp\|Q9SRY3\|RLF1_ARATH	4.0e-10	48.10	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
sp\|Q8L9P8\|RLF33_ARATH	2.0e-09	38.66	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of Bhi01G000843 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L9A8\|A0A0A0L9A8_CUCSA	3.0e-47	75.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1	[more]
tr\|A0A0A0L624\|A0A0A0L624_CUCSA	1.1e-28	72.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1	[more]
tr\|A0A1S3TB48\|A0A1S3TB48_VIGRR	2.4e-28	57.63	protein RALF-like 32 OS=Vigna radiata var. radiata OX=3916 GN=LOC106753652 PE=4 ...	[more]
tr\|A0A0S3R1D3\|A0A0S3R1D3_PHAAN	3.2e-28	56.78	Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G...	[more]
tr\|C6T1A5\|C6T1A5_SOYBN	9.2e-28	58.88	Uncharacterized protein OS=Glycine max OX=3847 GN=100500295 PE=2 SV=1	[more]

BLAST of Bhi01G000843 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147646.2	4.6e-47	75.78	PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical prote...	[more]
XP_023528073.1	3.0e-46	87.04	protein RALF-like 32 [Cucurbita pepo subsp. pepo]	[more]
XP_022956250.1	1.5e-45	86.11	protein RALF-like 32 [Cucurbita moschata]	[more]
XP_022138047.1	1.2e-42	79.17	protein RALF-like 32 [Momordica charantia]	[more]
KGN57228.1	1.6e-28	72.73	hypothetical protein Csa_3G171850 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0007267	cell-cell signaling
biological_process	GO:0008150	biological_process
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity
molecular_function	GO:0008430	selenium binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000843	Bhi01M000843	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 64..130 e-value: 2.5E-23 score: 82.6
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 26..132
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 26..132

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi01G000843	Csa3G171840	Cucumber (Chinese Long) v2	cuwgoB269
Bhi01G000843	MELO3C006773	Melon (DHL92) v3.5.1	mewgoB529
Bhi01G000843	CSPI03G15560	Wild cucumber (PI 183967)	cpiwgoB269
Bhi01G000843	Cucsa.251990	Cucumber (Gy14) v1	cgywgoB522
Bhi01G000843	CmaCh14G015430	Cucurbita maxima (Rimu)	cmawgoB0328
Bhi01G000843	CmoCh06G015950	Cucurbita moschata (Rifu)	cmowgoB1005
Bhi01G000843	CmoCh14G015780	Cucurbita moschata (Rifu)	cmowgoB0317
Bhi01G000843	Lsi05G013670	Bottle gourd (USVL1VR-Ls)	lsiwgoB444
Bhi01G000843	Cp4.1LG08g05550	Cucurbita pepo (Zucchini)	cpewgoB1110
Bhi01G000843	Cp4.1LG03g12150	Cucurbita pepo (Zucchini)	cpewgoB0783
Bhi01G000843	CsGy3G015610	Cucumber (Gy14) v2	cgybwgoB257
Bhi01G000843	MELO3C006773.2	Melon (DHL92) v3.6.1	medwgoB530
Bhi01G000843	Carg24781	Silver-seed gourd	carwgoB1220
Bhi01G000843	Carg15515	Silver-seed gourd	carwgoB0817
Bhi01G000843	CsaV3_3G015740	Cucumber (Chinese Long) v3	cucwgoB272
Bhi01G000843	Cla021850	Watermelon (97103) v1	wgowmB201
Bhi01G000843	Cla97C05G088730	Watermelon (97103) v2	wgowmbB178

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi01G000843	Cucurbita maxima (Rimu)	cmawgoB1006
Bhi01G000843	Watermelon (Charleston Gray)	wcgwgoB426