MELO3C006773.2 (gene) Melon (DHL92) v3.6.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C006773.2
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.6.1)
Description	Rapid ALkalinization Factor
Location	chr06 : 5864675 .. 5868520 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTACTAAAGAAATAAGGCAAAAAGGAAAAACTATCAAAAGTCAGAACAACAACATGAGAAGAAGAATCTCAAGCAAGCCAATGTCTTTACAACCTTGCTTTCTTCTTCTTCTTCTCTTTCTCTTCCTCCTTCAAGATCTTTGTCTTGTTCGAGCTTCCTCGACGCATTCTTGCAATGGCTCCATAGCTGAGTGTGGTAGCGAGGACGAGATTTTGATGGAGTCGGAGATAAGCCGAAGGTTTCTCGCTCAACAAAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCACCCAGCTTGCGACGGCGCTAGTGGTGGCCGACCTTACACCAAAAGTGGGAGTTGTGCTCCACCACCGGCTAATCCTTACGATCGAGGTTGCCCTAAGATATATCGTTGTAGGTCTGATGATTGAGGGTTTGATCAAACCATTATATGCTTGGTAGAATTTCTTCCCTTTTTTTTGTTCCTTTTTCTTCCTTTTTTCTTTTTTCTTTTCTGTGTGTTATTGTTAGGGAGGAGTATGATAGACCGAGAGATTGATCAACCGACCAAAACCAGTGAAACCAAAGGTGGTCGGTGTTGGTTCGGAAAAATCCAAACCAAGTTTTTTTAAAATCTTCAAATGCTTAAACCAATAGTCAACCAACCAATGATCAATTTGTACTTCTTCGTGGTTTAGGTCGATTTGAACATTTATTCAACCAACCAACTCTGACCAATGGCCACCCTAGTTCTCGTTGATTGATGAAGAGGTTTAATTATGAAATGAATGGAATTTTTCTTTTTCTTTGTGATCTCTTTTCTTTAGGAGATGATGTCATATTAGTTTCTGGAAAATAAAAAGAGATGAGAAATGTTAGACATGTTTGGTTGGATATCATGTTGTCCAGGATGTAAAGTGTGAGAATCACATCATACCTAATTTTATCTTATGTTGGGATTTATTCATTTTTAATCTACTTTTTCTTCCTTCCTTCCTTCCTTTATTTATTTATTTATTTTTTACGAAAAAGAAAAGTTTGGTAATAAATATGGTAGATTTTAAACCATAAACGGTTTACTAGAAAAGTGTAGTTTATCCATATTTATCTATATGTGATCAAATTACTTTGGTCGTACAACTACATTTTATTGTTTCTAACGTCCCCTTATTTTTAAATATTAAAACCCACTCTCTCGCCAACAGATAGTTCATGTAGTTCGATTTTTCTAATTTACATCTCTTGAACATAAAATTTTCGTTTCAATAGAACTTAGTTTATAATAAACAACAGTATAAATAATCTGCTAAATGAACTCTGGTATTCATATTGAATTCTCACTCCAGCTTTTAAGTACAACTCAACTAATTAAAATATTATATCATTAAAAAAAATGAAAATTCGAGTTCGGATCTTACAAATTGTTTAACTTTAAAAAAAAAAAAACTGTACCTTCCCCTTTCCTAAAAGAAAATTAAAGCAAAAACAAATCAGCTATCAAACACTTATTATATCAATGGGTTTGAACTTAAAAGGTCACAAAGTTTGATTCAATTGGATTATAGGGTAGGATGGGAGAAATCAGTTTAAGCTGAATAGAACTAGTGGTATGCATATATTACAAACAACTACAATATCTATTTGTTGTCTCTAATCTCCTTCATTTTCACGCTTTCAATAGCAACCACAGAATCGTTTCGTCTTTCTCTCTTGCTCACTTCACCTTGGCTGTAGCCCTTCGTTGCCACCCCCTCCTCCTCTTAGTTGTCAACCCCATCACCGCTGGGTGCTAATCACCATAGCATTCTCTTACAAGCCCTTTGCTTTAGCCCTCTCTCAATCATCGTCGAGTTCGCTCGACCTGAGAGAGTTGCCCACCATCTATCTCTTCCTCATTCATCATGCCCCACCTAACCCTTATTTTTGTTTTATGTACAAACCCACCCAAGACAGTCGAGAAATATATACACAGACACATACTACATATATTGAGCTCATTACTGATCTTAGCACGACCAAGTTACCAACTCTAAACTTATAACCAACTCTAAAATCCATTTTGAAGACAGGAAAGAAATCATGAAGGGCTCGTCCCTCATAACTCTACTATAAACGTATTCATTTTTTAATATTGAACTTATATACCTTATTTATTTTACACCGATTCAAAAAGTAAAAAACAAAACTGCCTCCATTCTGGTCATTTAGAAATTTGAATGTCTGTCATGTGGCGGTGGAAATTCAAACCTCTAGTCATGTAGTCTAAGGCATAATCTCTAATAGGTTGAACTACAGCTCAAGTTAGCACTCCCTGATTCAATTCTATGAGCTAACCTTTTGTTGATGATTACAGAAGGTTTTTGAAATCGAAGCCCAAGAACATCAAGGATAATTACCAAGTCAAGGTACATTAGTAATTAGCTAAGGAGTTCATTATACTTGTTTGTCAAATAAATGGATGAAAGGGAAGGAGAATGAAGAAGATAGGCAATATTTTAGAGTGATCTCAACATTGGAAGGGTCAAAGTACTTTGCATTACTTGGTTTAATCTTGTTGTTTAGTTAACTTTTATCTTTCAATATATTTTGGGTTCTATCCCAAGAAACATGAAGATATTTGATAATTTGTGAGAAGTTTTTGTAGAATAAAGGTTCTTTTGGAAAAGAGTCGATTGCCAGAGAGGGAAAAAATATGAATAAGAACTAATTTCATGTATATATCAGTATCCATAAATGAAAATAAGTTATTACATTAGCATTTATCTAAATGGAATTTCTCATATCACTTCCTTGTACAAGAAAATGAACATCAACTAAAAGAAAACTTATCCGTGAAAGACTAAGATTTCTAACCTATTTCAGTGAGAGCTTCCACACTTCTCTAACATTAGACGAACCATCAGCAACTTCCACAATCAGTTTTGAAACCATTTCACCTGTAAGGGGGCAGAAGATGAACAGTCAGTTAAAGAAAAAACAAAAAAATAAAAATTAATAAATAATAACAACGAGACTGAAGTGGTAACTATGAAAATAAATGAATATCAGTAATGCATGTAAACCTTTACTTGGCATGGCAACTAGTTCGAGGAAAATCTAAAATGTCACAAATATTATATCTCCTTGGCATGATCTAAACAAATGGAGCTGCAGGAGTAGTACTGTTGCTGATTACTAGAAGCATCCAAAACTACATGCTCCGATTGAAACTTTTAAATATTGTATTTTCACAAATTAGGAACAAGACTGAAGACAATCTTTTCTCAGATCAAACACTTTCTTAGCTATAAATTGTTGTAAATGTAGATCCCCTATTTGTAACCATAGCTTCACTAATCCGAAGGTTTTCTTGCAATATCAATGAAATTAATTCACTGTTTCCTATTAAAAAGATAAAAGAGCGTCGTCTTAAATTGGCCAATGACCTTCACCACATTCATCACTCATGATATTTTAGCTAGCAACGTGTATAGTTTTATCAAACTTCTTTTAATGTAGTTCTTCATCCAATCAGGAAGAAGTATGATGTTAAAGCATAATTTTATCAGGAAGCGTGAAAGAGAAAGACCAACTTACCTTCTTTTTCTAACACAGATAGAAAGCGCCGGGTTCCTCCTCCTACTCCAAAGTAATGTTTCTTTCCAGCCAAGTATACAACTCCATGAGGACGGCTCAAGCACTATAAGAGTGTCCAAAATTCCATTATGTAATGATACAATGAAATAATAGAAATACAACATTTAATCAGAAATTATATTTATAAGCATACAAAATTAGATGGACATGTGTATAAGTTCTACCTGTGTGATAAGCTTATATAGAGTTTTAAGTGTAGAAAGAGAATAAACTGTCTCTGCCA

mRNA sequence

CATTACTAAAGAAATAAGGCAAAAAGGAAAAACTATCAAAAGTCAGAACAACAACATGAGAAGAAGAATCTCAAGCAAGCCAATGTCTTTACAACCTTGCTTTCTTCTTCTTCTTCTCTTTCTCTTCCTCCTTCAAGATCTTTGTCTTGTTCGAGCTTCCTCGACGCATTCTTGCAATGGCTCCATAGCTGAGTGTGGTAGCGAGGACGAGATTTTGATGGAGTCGGAGATAAGCCGAAGGTTTCTCGCTCAACAAAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCACCCAGCTTGCGACGGCGCTAGTGGTGGCCGACCTTACACCAAAAGTGGGAGTTGTGCTCCACCACCGGCTAATCCTTACGATCGAGGTTGCCCTAAGATATATCGTTGTAGGTCTGATGATTGAGGGTTTGATCAAACCATTATATGCTTGAAGGTTTTTGAAATCGAAGCCCAAGAACATCAAGGATAATTACCAAGTCAAGATAGAAAGCGCCGGGTTCCTCCTCCTACTCCAAAGTAATGTTTCTTTCCAGCCAAGTATACAACTCCATGAGGACGGCTCAAGCACTATAAGAGTGTCCAAAATTCCATTATGTAATGATACAATGAAATAATAGAAATACAACATTTAATCAGAAATTATATTTATAAGCATACAAAATTAGATGGACATGTGTATAAGTTCTACCTGTGTGATAAGCTTATATAGAGTTTTAAGTGTAGAAAGAGAATAAACTGTCTCTGCCA

Coding sequence (CDS)

ATGAGAAGAAGAATCTCAAGCAAGCCAATGTCTTTACAACCTTGCTTTCTTCTTCTTCTTCTCTTTCTCTTCCTCCTTCAAGATCTTTGTCTTGTTCGAGCTTCCTCGACGCATTCTTGCAATGGCTCCATAGCTGAGTGTGGTAGCGAGGACGAGATTTTGATGGAGTCGGAGATAAGCCGAAGGTTTCTCGCTCAACAAAAGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCACCCAGCTTGCGACGGCGCTAGTGGTGGCCGACCTTACACCAAAAGTGGGAGTTGTGCTCCACCACCGGCTAATCCTTACGATCGAGGTTGCCCTAAGATATATCGTTGTAGGTCTGATGATTGA

Protein sequence

MRRRISSKPMSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD

Homology

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BLAST of MELO3C006773.2 vs. NCBI nr
Match: XP_004147646.2 (PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical protein Csa_3G171840 [Cucumis sativus])

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-46
Identity = 96/120 (80.00%), Postives = 104/120 (86.67%), Query Frame = 0


Query: 2   RRRISSKPMSLQPCFL-LLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEIS 61
           R+RISSKPMSLQ CFL           DLCLVRASSTHSCNGSIA C +E+E+LMESEI+
Sbjct: 9   RKRISSKPMSLQTCFLXXXXXXXXXXXDLCLVRASSTHSCNGSIAACANEEEMLMESEIT 68

Query: 62  RRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 121
           RRFL QQKKYISIGALKKDHPACDGASGG+PYT+SGSCAPPPANPY+RGC KIYRCRSDD
Sbjct: 69  RRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of MELO3C006773.2 vs. NCBI nr
Match: XP_022956250.1 (protein RALF-like 32 [Cucurbita moschata])

HSP 1 Score: 191.4 bits (485), Expect = 1.7e-45
Identity = 93/106 (87.74%), Postives = 96/106 (90.57%), Query Frame = 0


Query: 15  CFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKKYISIG 74
           CF   LLFL L+QDLCLVRASS HSCNGSIAECG EDE LMESEISRRFLAQQKKYISIG
Sbjct: 9   CF---LLFLLLVQDLCLVRASSKHSCNGSIAECGGEDETLMESEISRRFLAQQKKYISIG 68

Query: 75  ALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 121
           ALKKDHPACDG  GG+PYTKSGSCAPPPANPY+RGC KIYRCRSDD
Sbjct: 69  ALKKDHPACDGGGGGQPYTKSGSCAPPPANPYNRGCSKIYRCRSDD 111

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BLAST of MELO3C006773.2 vs. NCBI nr
Match: XP_023528073.1 (protein RALF-like 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.8 bits (473), Expect = 4.3e-44
Identity = 90/106 (84.91%), Postives = 95/106 (89.62%), Query Frame = 0


Query: 15  CFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKKYISIG 74
           CF   LLFL L+QDLCLVRASS HSCNGSIAECG E+E LMESEISRRFL QQKKYISIG
Sbjct: 9   CF---LLFLLLVQDLCLVRASSKHSCNGSIAECGGEEETLMESEISRRFLEQQKKYISIG 68

Query: 75  ALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 121
           ALKKDHPACDG  GG+PYT+SGSCAPPPANPY+RGC KIYRCRSDD
Sbjct: 69  ALKKDHPACDGGGGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of MELO3C006773.2 vs. NCBI nr
Match: XP_022138047.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-43
Identity = 93/120 (77.50%), Postives = 100/120 (83.33%), Query Frame = 0


Query: 1   MRRRISSKPMSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEIS 60
           MRRR+SSKP   QP FLLL   LFLLQ LCL+RAS    CNGSIAEC +E E+LMESEIS
Sbjct: 1   MRRRMSSKPKPSQPFFLLL---LFLLQALCLLRASFASPCNGSIAECNTEKEMLMESEIS 60

Query: 61  RRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 120
           RRFL QQKKYISIGALKKD PACDG+  G+PYTKSGSC PPP+NPYDRGC KIYRCRSDD
Sbjct: 61  RRFLEQQKKYISIGALKKDRPACDGSGRGQPYTKSGSCVPPPSNPYDRGCSKIYRCRSDD 117

[Close]

BLAST of MELO3C006773.2 vs. NCBI nr
Match: KGN57228.1 (hypothetical protein Csa_3G171850 [Cucumis sativus])

HSP 1 Score: 146.7 bits (369), Expect = 4.9e-32
Identity = 78/101 (77.23%), Postives = 84/101 (83.17%), Query Frame = 0


Query: 10  MSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKK 69
           MSLQ CFLLLL   FLLQDLCLVR SS HSCNGSIAEC +E+E+LMESEI+RRFLAQQKK
Sbjct: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60

Query: 70  -YISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRG 110
            YIS   LKKD PACDGAS G+PYTKSGSC P  ANPY+ G
Sbjct: 61  NYISYPTLKKDRPACDGAS-GQPYTKSGSCVPSQANPYNPG 100

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BLAST of MELO3C006773.2 vs. TAIR10
Match: AT4G14010.1 (ralf-like 32)

HSP 1 Score: 88.6 bits (218), Expect = 2.9e-18
Identity = 49/107 (45.79%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 18  LLLLFLFLLQDLCLVRASSTHSCNGSIAECGS-----EDEILMESEISRRFLAQQKKYIS 77
           + + FL LL      +ASS+  CNGS+AEC S     E  ++MES  S+R   +Q   +S
Sbjct: 12  ITIFFLCLLLAHVTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLS 71

Query: 78  IGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSD 120
            GAL+++ PACDG   G  Y  S  C PPP+NPY RGC K YRC  D
Sbjct: 72  YGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

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BLAST of MELO3C006773.2 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 64.7 bits (156), Expect = 4.4e-11
Identity = 42/93 (45.16%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 29  LCLVRASST---HSCNGSIAEC-GSEDEILMESEISRRFLAQQKKYISIGALKKDHPACD 88
           L  VRA S+     C GSIAEC   E+E+  +S+ISRR LA QKKYIS GA++++   C 
Sbjct: 31  LDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCS 90

Query: 89  GASGGRPYTKSGSCAPPPANPYDRGCPKIYRCR 118
                    + G+     ANPY RGC  I RCR
Sbjct: 91  RRGASYYNCQRGA----QANPYSRGCSTITRCR 118

[Close]

BLAST of MELO3C006773.2 vs. TAIR10
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 62.8 bits (151), Expect = 1.7e-10
Identity = 37/79 (46.84%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 40  CNGSIAECGSEDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCA 99
           C+GSIAEC   +E  M+SEI+RR LA   KYIS  +LK++   C          ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILA-TTKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 100 PPPANPYDRGCPKIYRCRS 119
              ANPY RGC KI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of MELO3C006773.2 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 58.9 bits (141), Expect = 2.4e-09
Identity = 44/119 (36.97%), Postives = 61/119 (51.26%), Query Frame = 0


Query: 3   RRISSKPMSLQPCFLLL-LLFLFLLQDLCLVRASSTHSCNGSIAECG---SEDEILMESE 62
           R +S+KP+++    L +  LF  +              CNG+IAEC    +E+E  M+SE
Sbjct: 2   RGLSTKPVAIIIAILTVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSE 61

Query: 63  ISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCR 118
           I+RR LA   KYIS GAL+++   C          + G+     ANPY RGC  I RCR
Sbjct: 62  INRRILA-TTKYISYGALRRNTVPCSRRGASYYNCRRGA----QANPYSRGCSAITRCR 115

[Close]

BLAST of MELO3C006773.2 vs. TAIR10
Match: AT4G13950.1 (ralf-like 31)

HSP 1 Score: 52.8 bits (125), Expect = 1.7e-07
Identity = 30/70 (42.86%), Postives = 35/70 (50.00%), Query Frame = 0


Query: 50  EDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRG 109
           EDE LM +EISRR L  QK+YI    L++D   C      +P      C    AN Y RG
Sbjct: 43  EDEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQ-----KPGASYYDCRSGQANSYSRG 102

Query: 110 CPKIYRCRSD 120
           C  I RC  D
Sbjct: 103 CDTITRCARD 107

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BLAST of MELO3C006773.2 vs. Swiss-Prot
Match: sp|O23262|RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.2e-17
Identity = 49/107 (45.79%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 18  LLLLFLFLLQDLCLVRASSTHSCNGSIAECGS-----EDEILMESEISRRFLAQQKKYIS 77
           + + FL LL      +ASS+  CNGS+AEC S     E  ++MES  S+R   +Q   +S
Sbjct: 12  ITIFFLCLLLAHVTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLS 71

Query: 78  IGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSD 120
            GAL+++ PACDG   G  Y  S  C PPP+NPY RGC K YRC  D
Sbjct: 72  YGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of MELO3C006773.2 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.0e-10
Identity = 42/93 (45.16%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 29  LCLVRASST---HSCNGSIAEC-GSEDEILMESEISRRFLAQQKKYISIGALKKDHPACD 88
           L  VRA S+     C GSIAEC   E+E+  +S+ISRR LA QKKYIS GA++++   C 
Sbjct: 31  LDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCS 90

Query: 89  GASGGRPYTKSGSCAPPPANPYDRGCPKIYRCR 118
                    + G+     ANPY RGC  I RCR
Sbjct: 91  RRGASYYNCQRGA----QANPYSRGCSTITRCR 118

[Close]

BLAST of MELO3C006773.2 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-09
Identity = 40/86 (46.51%), Postives = 47/86 (54.65%), Query Frame = 0


Query: 34  ASSTHSCNGSIAEC-GSEDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPY 93
           A S   C GSI EC   E+E  ++SE +RR LA  KKYIS GAL+K+   C         
Sbjct: 35  ARSGGGCKGSIGECIAEEEEFELDSESNRRILA-TKKYISYGALQKNSVPCSRRGASYYN 94

Query: 94  TKSGSCAPPPANPYDRGCPKIYRCRS 119
            K G+     ANPY RGC  I RCRS
Sbjct: 95  CKPGA----QANPYSRGCSAITRCRS 115

[Close]

BLAST of MELO3C006773.2 vs. Swiss-Prot
Match: sp|Q9SRY3|RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.0e-09
Identity = 37/79 (46.84%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 40  CNGSIAECGSEDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCA 99
           C+GSIAEC   +E  M+SEI+RR LA   KYIS  +LK++   C          ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILA-TTKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 100 PPPANPYDRGCPKIYRCRS 119
              ANPY RGC KI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of MELO3C006773.2 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.4e-08
Identity = 44/119 (36.97%), Postives = 61/119 (51.26%), Query Frame = 0


Query: 3   RRISSKPMSLQPCFLLL-LLFLFLLQDLCLVRASSTHSCNGSIAECG---SEDEILMESE 62
           R +S+KP+++    L +  LF  +              CNG+IAEC    +E+E  M+SE
Sbjct: 2   RGLSTKPVAIIIAILTVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSE 61

Query: 63  ISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCR 118
           I+RR LA   KYIS GAL+++   C          + G+     ANPY RGC  I RCR
Sbjct: 62  INRRILA-TTKYISYGALRRNTVPCSRRGASYYNCRRGA----QANPYSRGCSAITRCR 115

[Close]

BLAST of MELO3C006773.2 vs. TrEMBL
Match: tr|A0A0A0L9A8|A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-46
Identity = 96/120 (80.00%), Postives = 104/120 (86.67%), Query Frame = 0


Query: 2   RRRISSKPMSLQPCFL-LLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEIS 61
           R+RISSKPMSLQ CFL           DLCLVRASSTHSCNGSIA C +E+E+LMESEI+
Sbjct: 9   RKRISSKPMSLQTCFLXXXXXXXXXXXDLCLVRASSTHSCNGSIAACANEEEMLMESEIT 68

Query: 62  RRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSDD 121
           RRFL QQKKYISIGALKKDHPACDGASGG+PYT+SGSCAPPPANPY+RGC KIYRCRSDD
Sbjct: 69  RRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of MELO3C006773.2 vs. TrEMBL
Match: tr|A0A0A0L624|A0A0A0L624_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.2e-32
Identity = 78/101 (77.23%), Postives = 84/101 (83.17%), Query Frame = 0


Query: 10  MSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKK 69
           MSLQ CFLLLL   FLLQDLCLVR SS HSCNGSIAEC +E+E+LMESEI+RRFLAQQKK
Sbjct: 1   MSLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKK 60

Query: 70  -YISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRG 110
            YIS   LKKD PACDGAS G+PYTKSGSC P  ANPY+ G
Sbjct: 61  NYISYPTLKKDRPACDGAS-GQPYTKSGSCVPSQANPYNPG 100

[Close]

BLAST of MELO3C006773.2 vs. TrEMBL
Match: tr|A0A2P6QW25|A0A2P6QW25_ROSCH (Putative rapid ALkalinization Factor OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0413341 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-27
Identity = 70/110 (63.64%), Postives = 82/110 (74.55%), Query Frame = 0


Query: 10  MSLQPCFLLLLLFLFLLQDLCLVRASSTHSCNGSIAECGSEDEILMESEISRRFLAQQKK 69
           +SLQ CF+LLLL L  L       +SST  CNGSIAEC +E E+LM+SEISRRFLAQ  K
Sbjct: 2   VSLQSCFVLLLLCLSYLSATASPPSSST--CNGSIAECETESELLMDSEISRRFLAQ--K 61

Query: 70  YISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSD 120
           YIS GAL+KD P C+G + G  Y+K+G C PPPANPY+RGC K YRCRSD
Sbjct: 62  YISPGALRKDQPVCNGGARGEAYSKTGGCLPPPANPYNRGCSKYYRCRSD 107

[Close]

BLAST of MELO3C006773.2 vs. TrEMBL
Match: tr|A0A0S3R1D3|A0A0S3R1D3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G214200 PE=4 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-27
Identity = 65/118 (55.08%), Postives = 86/118 (72.88%), Query Frame = 0


Query: 5   ISSKPMSL-QPCFLLLLLFLFLLQDLCLVRASSTH--SCNGSIAECGSEDEILMESEISR 64
           ++SKP ++ + CF+ L+ F F+    C V + ++H  +CNGSIAEC  EDE+LMESE SR
Sbjct: 1   MASKPSTIVRLCFIYLIFFSFMRLSSCTVLSLTSHVSTCNGSIAECNQEDELLMESETSR 60

Query: 65  RFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSD 120
           RFL +QKKYIS GAL++D P C+G   G  Y+ SG C PPP+NP++RGC K YRCRSD
Sbjct: 61  RFL-EQKKYISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRGCSKYYRCRSD 117

[Close]

BLAST of MELO3C006773.2 vs. TrEMBL
Match: tr|A0A1S3TB48|A0A1S3TB48_VIGRR (protein RALF-like 32 OS=Vigna radiata var. radiata OX=3916 GN=LOC106753652 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-27
Identity = 66/118 (55.93%), Postives = 85/118 (72.03%), Query Frame = 0


Query: 5   ISSKPMS-LQPCFLLLLLFLFLLQDLCLVRASSTH--SCNGSIAECGSEDEILMESEISR 64
           ++SKP + ++ CF  L+ F F+    C V + + H  +CNGSIAEC  EDE+LMESEISR
Sbjct: 1   MASKPSTIIRLCFFYLMFFGFMRLSSCTVLSLTPHVSTCNGSIAECNQEDELLMESEISR 60

Query: 65  RFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIYRCRSD 120
           RFL +QKKYIS GAL++D P C+G   G  Y+ SG C PPP+NP++RGC K YRCRSD
Sbjct: 61  RFL-EQKKYISNGALQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRGCSKYYRCRSD 117

The following BLAST results are available for this feature:

BLAST of MELO3C006773.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147646.2	2.1e-46	80.00	PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical prote...	[more]
XP_022956250.1	1.7e-45	87.74	protein RALF-like 32 [Cucurbita moschata]	[more]
XP_023528073.1	4.3e-44	84.91	protein RALF-like 32 [Cucurbita pepo subsp. pepo]	[more]
XP_022138047.1	2.1e-43	77.50	protein RALF-like 32 [Momordica charantia]	[more]
KGN57228.1	4.9e-32	77.23	hypothetical protein Csa_3G171850 [Cucumis sativus]	[more]

BLAST of MELO3C006773.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14010.1	2.9e-18	45.79	ralf-like 32	[more]
AT3G05490.1	4.4e-11	45.16	ralf-like 22	[more]
AT1G02900.1	1.7e-10	46.84	rapid alkalinization factor 1	[more]
AT4G15800.1	2.4e-09	36.97	ralf-like 33	[more]
AT4G13950.1	1.7e-07	42.86	ralf-like 31	[more]

BLAST of MELO3C006773.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O23262\|RLF32_ARATH	5.2e-17	45.79	Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1	[more]
sp\|Q9MA62\|RLF22_ARATH	8.0e-10	45.16	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
sp\|Q945T0\|RALF_TOBAC	1.4e-09	46.51	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
sp\|Q9SRY3\|RLF1_ARATH	3.0e-09	46.84	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
sp\|Q8L9P8\|RLF33_ARATH	4.4e-08	36.97	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of MELO3C006773.2 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L9A8\|A0A0A0L9A8_CUCSA	1.4e-46	80.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1	[more]
tr\|A0A0A0L624\|A0A0A0L624_CUCSA	3.2e-32	77.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1	[more]
tr\|A0A2P6QW25\|A0A2P6QW25_ROSCH	1.1e-27	63.64	Putative rapid ALkalinization Factor OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4...	[more]
tr\|A0A0S3R1D3\|A0A0S3R1D3_PHAAN	1.8e-27	55.08	Uncharacterized protein OS=Vigna angularis var. angularis OX=157739 GN=Vigan.01G...	[more]
tr\|A0A1S3TB48\|A0A1S3TB48_VIGRR	2.4e-27	55.93	protein RALF-like 32 OS=Vigna radiata var. radiata OX=3916 GN=LOC106753652 PE=4 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0008430	selenium binding
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C006773.2.1	MELO3C006773.2.1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 52..117 e-value: 1.6E-23 score: 83.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..108
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 12..119
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 12..119

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C006773.2	Csa3G171840	Cucumber (Chinese Long) v2	cumedB223
MELO3C006773.2	CSPI03G15560	Wild cucumber (PI 183967)	cpimedB224
MELO3C006773.2	Cucsa.251990	Cucumber (Gy14) v1	cgymedB432
MELO3C006773.2	CmaCh14G015430	Cucurbita maxima (Rimu)	cmamedB273
MELO3C006773.2	CmoCh06G015950	Cucurbita moschata (Rifu)	cmomedB857
MELO3C006773.2	CmoCh14G015780	Cucurbita moschata (Rifu)	cmomedB260
MELO3C006773.2	Lsi05G013670	Bottle gourd (USVL1VR-Ls)	lsimedB397
MELO3C006773.2	Cp4.1LG08g05550	Cucurbita pepo (Zucchini)	cpemedB951
MELO3C006773.2	Cp4.1LG03g12260	Cucurbita pepo (Zucchini)	cpemedB693
MELO3C006773.2	CsGy3G015610	Cucumber (Gy14) v2	cgybmedB201
MELO3C006773.2	Cla021850	Watermelon (97103) v1	medwmB447
MELO3C006773.2	Cla97C05G088730	Watermelon (97103) v2	medwmbB423
MELO3C006773.2	Bhi01G000843	Wax gourd	medwgoB530
MELO3C006773.2	Carg15515	Silver-seed gourd	carmedB0706
MELO3C006773.2	Carg24781	Silver-seed gourd	carmedB1020
MELO3C006773.2	CsaV3_3G015740	Cucumber (Chinese Long) v3	cucmedB230

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C006773.2	Cucurbita maxima (Rimu)	cmamedB863
MELO3C006773.2	Watermelon (Charleston Gray)	medwcgB416
MELO3C006773.2	Silver-seed gourd	carmedB0292