CsaV3_3G015740 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_3G015740
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Rapid ALkalinization Factor
Location	chr3 : 11696240 .. 11698130 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGGTTGCTCTAACATATATTATTGTAGGCATTGATTAAGGGTTTGATCAAACCATTGTATTCGTCGTAGAATCTCTTCCCTTCCCCCCCCCCCCCCCCTTTTTTTTGTGTGCGTGTTATTATCAACCAACCAAAATTGACAAAACTAAAAGTGATCAGATGTGAGAGATAAGGAGAATTCGATTGGTGTGTCGGTCTGGAAAAATCCAAACCGACATTTTTTTTAATCTTCAAATGCTTAAACCGACTATCAACCAACCAACGATCAATTTATATTTCTTCATGGTCAAATATTGGTTTGAACATTTACTAAACGACCATAATCGATTCGACAATGATTTGGCTAGAAAACCGACTTCAACGCCAGCCCTAGTCTTTGTTGATCAATGAAGAGGATTAATTATGAAATCAATGGAAATTTTCTTTTCTTCGCGATCTCTTTTTTTTAGGAGGTGGTGTCATATTCGTTTTTTGGAAAATAAAAAAGTGATCACAAGTTACTTAAGTAAAGAATGGCCAACATGAATATGACTCAATTAGTATAAGACATACACTCTCGATTGAGAAGTTTGAACCCTCTATCTCTAACATAGTTGCACTAAAAAATTAACACCACGAATTGATAAGACAGACAAAGTTGCACAAACGGACTCACGCGTGAGACAAGTTGCAAATCCAAAATGGTTGTTTTTCTTTTATTAAATGGAATCAGACGTGACATGTTCTTTTAATAATATATAGTCAGATAAAAGGAATCTAATCATATAATTAAGGTAAAGGATTTATATACAGTAATATTATTAAAGAATACCCTTTTTAAGTATTTGATACTACAAGTTTTTCTCAACAATGTATTAAGTAGAAATTTCATTTCAAGTCTCTTATTGAGGGAATAAAATTGGATATAGCACAAAATACAATATGAAATTGAAAAACAATATATATATATATATATATATATATATATATATATATATATATAATACATTGACTCACCTACCCCTTAATTATTTTTTTGCATCACAAATGTACCAAAAAAAATTCAACTTTCACCATGGAAAAGCAAATACTCAAAAGCAATTGGGTAGGTTTTTTATTTTTTATTTTTATTGAAATTTTGTCCCTTTAATTCCCATTGATGGTTCTTCCACTAATGAATATTTCTCAATTTTTTTTCATCCATATAAAATTTGAAAAAGAAATGAAAAGAAAAAAAACAAAATTTGAAGGAATAATATCTATAAAGTTTCTTCCCATTAGTAAAGAAATAAGGCAAAAGAGAAAAGAAGTATGGAAAGTCAGAATAACAACACGAGAAAAAGAATCTCAAGCAAGCCAATGTCTTTACAGACTTGCTTTCTTCTTCTTCTCTTTCTCTTCCTCTTCCTCTTTCAAGATCTTTGTCTCGTTCGAGCTTCCTCCACACATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCAGAGATAACTCGAAGGTTTCTCGAACAACAAAAGAAATACATCTCTATTGGAGCTTTAAAGAAGGATCACCCCGCTTGCGATGGCGCTAGTGGTGGCCAACCTTACACCAGAAGTGGAAGTTGTGCTCCGCCACCGGCTAATCCTTACAATCGAGGTTGCTCTAAGATATATCGTTGTAGGTCTGATGATTGA

mRNA sequence

ATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGATCTTTGTCTCGTTCGAGCTTCCTCCACACATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCAGAGATAACTCGAAGGTTTCTCGAACAACAAAAGAAATACATCTCTATTGGAGCTTTAAAGAAGGATCACCCCGCTTGCGATGGCGCTAGTGGTGGCCAACCTTACACCAGAAGTGGAAGTTGTGCTCCGCCACCGGCTAATCCTTACAATCGAGGTTGCTCTAAGATATATCGTTGTAGGTCTGATGATTGA

Coding sequence (CDS)

ATGCATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCGGAGATAACTCGAAGGTTTCTCGCACAACAAAAGAAGAACTACATCTCTTATCCAACTTTGAAGAAGGATCGCCCAGCTTGCGATGGCGCTAGTGGCCAACCTTACACTAAGAGCGGAAGTTGCGTTCCGTCACAGGCTAATCCTTACAATCCAGATCTTTGTCTCGTTCGAGCTTCCTCCACACATTCTTGCAATGGCTCCATAGCCGAGTGTGCTAACGAGGAGGAGATGTTGATGGAGTCAGAGATAACTCGAAGGTTTCTCGAACAACAAAAGAAATACATCTCTATTGGAGCTTTAAAGAAGGATCACCCCGCTTGCGATGGCGCTAGTGGTGGCCAACCTTACACCAGAAGTGGAAGTTGTGCTCCGCCACCGGCTAATCCTTACAATCGAGGTTGCTCTAAGATATATCGTTGTAGGTCTGATGATTGA

Protein sequence

MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYNPDLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD

Homology

[Close]

BLAST of CsaV3_3G015740 vs. NCBI nr
Match: XP_004147646.2 (PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical protein Csa_3G171840 [Cucumis sativus])

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-46
Identity = 92/93 (98.92%), Postives = 92/93 (98.92%), Query Frame = 0


Query: 73  DLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 132
           DLCLVRASSTHSCNGSIA CANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS
Sbjct: 36  DLCLVRASSTHSCNGSIAACANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 95

Query: 133 GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 166
           GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD
Sbjct: 96  GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of CsaV3_3G015740 vs. NCBI nr
Match: XP_023528073.1 (protein RALF-like 32 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 184.5 bits (467), Expect = 2.9e-43
Identity = 86/93 (92.47%), Postives = 87/93 (93.55%), Query Frame = 0


Query: 73  DLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 132
           DLCLVRASS HSCNGSIAEC  EEE LMESEI+RRFLEQQKKYISIGALKKDHPACDG  
Sbjct: 19  DLCLVRASSKHSCNGSIAECGGEEETLMESEISRRFLEQQKKYISIGALKKDHPACDGGG 78

Query: 133 GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 166
           GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD
Sbjct: 79  GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of CsaV3_3G015740 vs. NCBI nr
Match: XP_022956250.1 (protein RALF-like 32 [Cucurbita moschata])

HSP 1 Score: 179.9 bits (455), Expect = 7.2e-42
Identity = 83/93 (89.25%), Postives = 86/93 (92.47%), Query Frame = 0


Query: 73  DLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 132
           DLCLVRASS HSCNGSIAEC  E+E LMESEI+RRFL QQKKYISIGALKKDHPACDG  
Sbjct: 19  DLCLVRASSKHSCNGSIAECGGEDETLMESEISRRFLAQQKKYISIGALKKDHPACDGGG 78

Query: 133 GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 166
           GGQPYT+SGSCAPPPANPYNRGCSKIYRCRSDD
Sbjct: 79  GGQPYTKSGSCAPPPANPYNRGCSKIYRCRSDD 111

[Close]

BLAST of CsaV3_3G015740 vs. NCBI nr
Match: XP_022138047.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 162.2 bits (409), Expect = 1.6e-36
Identity = 75/92 (81.52%), Postives = 82/92 (89.13%), Query Frame = 0


Query: 74  LCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASG 133
           LCL+RAS    CNGSIAEC  E+EMLMESEI+RRFLEQQKKYISIGALKKD PACDG+  
Sbjct: 26  LCLLRASFASPCNGSIAECNTEKEMLMESEISRRFLEQQKKYISIGALKKDRPACDGSGR 85

Query: 134 GQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 166
           GQPYT+SGSC PPP+NPY+RGCSKIYRCRSDD
Sbjct: 86  GQPYTKSGSCVPPPSNPYDRGCSKIYRCRSDD 117

[Close]

BLAST of CsaV3_3G015740 vs. NCBI nr
Match: KGN57228.1 (hypothetical protein Csa_3G171850 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 9.5e-34
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1   MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG 60
           MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG
Sbjct: 28  MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG 87

Query: 61  SCVPSQANPYNPDL 75
           SCVPSQANPYNP +
Sbjct: 88  SCVPSQANPYNPGI 101

[Close]

BLAST of CsaV3_3G015740 vs. TAIR10
Match: AT4G14010.1 (ralf-like 32)

HSP 1 Score: 91.7 bits (226), Expect = 4.7e-19
Identity = 48/102 (47.06%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 74  LCLV------RASSTHSCNGSIAECAN-----EEEMLMESEITRRFLEQQKKYISIGALK 133
           LCL+      +ASS+  CNGS+AEC++     E  ++MES  ++R  E+Q   +S GAL+
Sbjct: 17  LCLLLAHVTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALR 76

Query: 134 KDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSD 165
           ++ PACDG   G+ Y  S  C PPP+NPY+RGCSK YRC  D
Sbjct: 77  RNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of CsaV3_3G015740 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 69.3 bits (168), Expect = 2.5e-12
Identity = 44/97 (45.36%), Postives = 56/97 (57.73%), Query Frame = 0


Query: 70  YNPDLCLVRASST---HSCNGSIAEC-ANEEEMLMESEITRRFLEQQKKYISIGALKKDH 129
           +   L  VRA S+     C GSIAEC A EEEM  +S+I+RR L  QKKYIS GA++++ 
Sbjct: 27  FGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNS 86

Query: 130 PACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCR 163
             C          + G+     ANPY+RGCS I RCR
Sbjct: 87  VPCSRRGASYYNCQRGA----QANPYSRGCSTITRCR 118

[Close]

BLAST of CsaV3_3G015740 vs. TAIR10
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 65.5 bits (158), Expect = 3.6e-11
Identity = 38/79 (48.10%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 85  CNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCA 144
           C+GSIAEC   EE  M+SEI RR L    KYIS  +LK++   C          ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRIL-ATTKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 145 PPPANPYNRGCSKIYRCRS 164
              ANPY+RGCSKI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of CsaV3_3G015740 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 63.5 bits (153), Expect = 1.4e-10
Identity = 39/81 (48.15%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 85  CNGSIAECA---NEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSG 144
           CNG+IAEC+    EEE  M+SEI RR L    KYIS GAL+++   C          R G
Sbjct: 40  CNGTIAECSLSTAEEEFEMDSEINRRIL-ATTKYISYGALRRNTVPCSRRGASYYNCRRG 99

Query: 145 SCAPPPANPYNRGCSKIYRCR 163
           +     ANPY+RGCS I RCR
Sbjct: 100 A----QANPYSRGCSAITRCR 115

[Close]

BLAST of CsaV3_3G015740 vs. TAIR10
Match: AT3G23805.1 (ralf-like 24)

HSP 1 Score: 54.3 bits (129), Expect = 8.3e-08
Identity = 34/79 (43.04%), Postives = 41/79 (51.90%), Query Frame = 0


Query: 86  NGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAP 145
           NG I E   E E +M SEI+RR +  +K+YIS   L++D   C          RSG    
Sbjct: 41  NGVIGE--EEGEEMMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQ--- 100

Query: 146 PPANPYNRGCSKIYRCRSD 165
             AN YNRGCS I RC  D
Sbjct: 101 --ANAYNRGCSVITRCARD 112

[Close]

BLAST of CsaV3_3G015740 vs. Swiss-Prot
Match: sp|O23262|RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.5e-18
Identity = 48/102 (47.06%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 74  LCLV------RASSTHSCNGSIAECAN-----EEEMLMESEITRRFLEQQKKYISIGALK 133
           LCL+      +ASS+  CNGS+AEC++     E  ++MES  ++R  E+Q   +S GAL+
Sbjct: 17  LCLLLAHVTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALR 76

Query: 134 KDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSD 165
           ++ PACDG   G+ Y  S  C PPP+NPY+RGCSK YRC  D
Sbjct: 77  RNQPACDGGKRGESY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of CsaV3_3G015740 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.5e-11
Identity = 44/97 (45.36%), Postives = 56/97 (57.73%), Query Frame = 0


Query: 70  YNPDLCLVRASST---HSCNGSIAEC-ANEEEMLMESEITRRFLEQQKKYISIGALKKDH 129
           +   L  VRA S+     C GSIAEC A EEEM  +S+I+RR L  QKKYIS GA++++ 
Sbjct: 27  FGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNS 86

Query: 130 PACDGASGGQPYTRSGSCAPPPANPYNRGCSKIYRCR 163
             C          + G+     ANPY+RGCS I RCR
Sbjct: 87  VPCSRRGASYYNCQRGA----QANPYSRGCSTITRCR 118

[Close]

BLAST of CsaV3_3G015740 vs. Swiss-Prot
Match: sp|Q9SRY3|RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.5e-10
Identity = 38/79 (48.10%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 85  CNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCA 144
           C+GSIAEC   EE  M+SEI RR L    KYIS  +LK++   C          ++G+  
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRIL-ATTKYISYQSLKRNSVPCSRRGASYYNCQNGA-- 106

Query: 145 PPPANPYNRGCSKIYRCRS 164
              ANPY+RGCSKI RCRS
Sbjct: 107 --QANPYSRGCSKIARCRS 120

[Close]

BLAST of CsaV3_3G015740 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-09
Identity = 44/87 (50.57%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 79  ASSTHSCNGSIAEC-ANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPY 138
           A S   C GSI EC A EEE  ++SE  RR L   KKYIS GAL+K+   C    G   Y
Sbjct: 35  ARSGGGCKGSIGECIAEEEEFELDSESNRRIL-ATKKYISYGALQKNSVPC-SRRGASYY 94

Query: 139 TRSGSCAP-PPANPYNRGCSKIYRCRS 164
               +C P   ANPY+RGCS I RCRS
Sbjct: 95  ----NCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of CsaV3_3G015740 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 39/81 (48.15%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 85  CNGSIAECA---NEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSG 144
           CNG+IAEC+    EEE  M+SEI RR L    KYIS GAL+++   C          R G
Sbjct: 40  CNGTIAECSLSTAEEEFEMDSEINRRIL-ATTKYISYGALRRNTVPCSRRGASYYNCRRG 99

Query: 145 SCAPPPANPYNRGCSKIYRCR 163
           +     ANPY+RGCS I RCR
Sbjct: 100 A----QANPYSRGCSAITRCR 115

[Close]

BLAST of CsaV3_3G015740 vs. TrEMBL
Match: tr|A0A0A0L9A8|A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-46
Identity = 92/93 (98.92%), Postives = 92/93 (98.92%), Query Frame = 0


Query: 73  DLCLVRASSTHSCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 132
           DLCLVRASSTHSCNGSIA CANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS
Sbjct: 36  DLCLVRASSTHSCNGSIAACANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 95

Query: 133 GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 166
           GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD
Sbjct: 96  GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of CsaV3_3G015740 vs. TrEMBL
Match: tr|A0A0A0L624|A0A0A0L624_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.3e-34
Identity = 72/74 (97.30%), Postives = 73/74 (98.65%), Query Frame = 0


Query: 1   MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG 60
           MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG
Sbjct: 28  MHSCNGSIAECANEEEMLMESEITRRFLAQQKKNYISYPTLKKDRPACDGASGQPYTKSG 87

Query: 61  SCVPSQANPYNPDL 75
           SCVPSQANPYNP +
Sbjct: 88  SCVPSQANPYNPGI 101

[Close]

BLAST of CsaV3_3G015740 vs. TrEMBL
Match: tr|B7FMN1|B7FMN1_MEDTR (RALF OS=Medicago truncatula OX=3880 GN=11417972 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.4e-25
Identity = 59/93 (63.44%), Postives = 72/93 (77.42%), Query Frame = 0


Query: 76  LVRASSTHS----CNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGA 135
           ++  SS H     CNGSIAEC  E+E LMESEI+RRFLE Q++YIS GALK+D P C+G 
Sbjct: 27  VISLSSNHHHASICNGSIAECNQEDEQLMESEISRRFLE-QRRYISEGALKRDKPVCNGG 86

Query: 136 SGGQPYTRSGSCAPPPANPYNRGCSKIYRCRSD 165
           +GG+ Y++S  C PPP+NPYNRGCSK YRCRSD
Sbjct: 87  AGGEAYSKSAGCIPPPSNPYNRGCSKYYRCRSD 118

[Close]

BLAST of CsaV3_3G015740 vs. TrEMBL
Match: tr|C6T1A5|C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100500295 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-25
Identity = 56/92 (60.87%), Postives = 72/92 (78.26%), Query Frame = 0


Query: 75  CLVRASSTH--SCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGAS 134
           C V +  +H  +CNGSIAEC  E+E+LMESEI+RRFLEQ++ YIS GAL++D P C+G  
Sbjct: 27  CTVFSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQRDKPVCNGGG 86

Query: 135 GGQPYTRSGSCAPPPANPYNRGCSKIYRCRSD 165
            G+ Y+++G C PPP+NP NRGCSK YRCRSD
Sbjct: 87  SGEAYSKTGGCLPPPSNPQNRGCSKYYRCRSD 118

[Close]

BLAST of CsaV3_3G015740 vs. TrEMBL
Match: tr|I3RZ93|I3RZ93_LOTJA (Uncharacterized protein OS=Lotus japonicus OX=34305 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.4e-25
Identity = 55/81 (67.90%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 84  SCNGSIAECANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSC 143
           +CNGSIAEC  E+E+LMESEI+RRFLE +++YIS GALK+D P C+G + G+ Y++SG C
Sbjct: 33  TCNGSIAECNQEDELLMESEISRRFLE-ERRYISPGALKRDKPVCNGGASGEAYSKSGGC 92

Query: 144 APPPANPYNRGCSKIYRCRSD 165
            PPP+NPYNRGCSK YRCRSD
Sbjct: 93  LPPPSNPYNRGCSKYYRCRSD 112

The following BLAST results are available for this feature:

BLAST of CsaV3_3G015740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147646.2	1.7e-46	98.92	PREDICTED: protein RALF-like 32 [Cucumis sativus] >KGN57227.1 hypothetical prote...	[more]
XP_023528073.1	2.9e-43	92.47	protein RALF-like 32 [Cucurbita pepo subsp. pepo]	[more]
XP_022956250.1	7.2e-42	89.25	protein RALF-like 32 [Cucurbita moschata]	[more]
XP_022138047.1	1.6e-36	81.52	protein RALF-like 32 [Momordica charantia]	[more]
KGN57228.1	9.5e-34	97.30	hypothetical protein Csa_3G171850 [Cucumis sativus]	[more]

BLAST of CsaV3_3G015740 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14010.1	4.7e-19	47.06	ralf-like 32	[more]
AT3G05490.1	2.5e-12	45.36	ralf-like 22	[more]
AT1G02900.1	3.6e-11	48.10	rapid alkalinization factor 1	[more]
AT4G15800.1	1.4e-10	48.15	ralf-like 33	[more]
AT3G23805.1	8.3e-08	43.04	ralf-like 24	[more]

BLAST of CsaV3_3G015740 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O23262\|RLF32_ARATH	8.5e-18	47.06	Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1	[more]
sp\|Q9MA62\|RLF22_ARATH	4.5e-11	45.36	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
sp\|Q9SRY3\|RLF1_ARATH	6.5e-10	48.10	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
sp\|Q945T0\|RALF_TOBAC	1.4e-09	50.57	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
sp\|Q8L9P8\|RLF33_ARATH	2.5e-09	48.15	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of CsaV3_3G015740 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L9A8\|A0A0A0L9A8_CUCSA	1.1e-46	98.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171840 PE=4 SV=1	[more]
tr\|A0A0A0L624\|A0A0A0L624_CUCSA	6.3e-34	97.30	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171850 PE=4 SV=1	[more]
tr\|B7FMN1\|B7FMN1_MEDTR	1.4e-25	63.44	RALF OS=Medicago truncatula OX=3880 GN=11417972 PE=2 SV=1	[more]
tr\|C6T1A5\|C6T1A5_SOYBN	1.8e-25	60.87	Uncharacterized protein OS=Glycine max OX=3847 GN=100500295 PE=2 SV=1	[more]
tr\|I3RZ93\|I3RZ93_LOTJA	2.4e-25	67.90	Uncharacterized protein OS=Lotus japonicus OX=34305 PE=2 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G015740.1	CsaV3_3G015740.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 92..112
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..153
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 78..164
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 3..71
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 78..164
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 3..71
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 96..162 e-value: 2.0E-24 score: 86.1 coord: 15..71 e-value: 1.9E-15 score: 57.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_3G015740	CSPI03G15560	Wild cucumber (PI 183967)	cpicucB144
CsaV3_3G015740	Cucsa.251990	Cucumber (Gy14) v1	cgycucB387
CsaV3_3G015740	CmaCh14G015430	Cucurbita maxima (Rimu)	cmacucB0291
CsaV3_3G015740	CmoCh14G015780	Cucurbita moschata (Rifu)	cmocucB0283
CsaV3_3G015740	CmoCh06G015950	Cucurbita moschata (Rifu)	cmocucB0953
CsaV3_3G015740	Cp4.1LG03g12260	Cucurbita pepo (Zucchini)	cpecucB0759
CsaV3_3G015740	Cp4.1LG08g05550	Cucurbita pepo (Zucchini)	cpecucB1056
CsaV3_3G015740	CsGy3G015610	Cucumber (Gy14) v2	cgybcucB117
CsaV3_3G015740	Carg15515	Silver-seed gourd	carcucB0731
CsaV3_3G015740	Carg24781	Silver-seed gourd	carcucB1087
CsaV3_3G015740	Cla021850	Watermelon (97103) v1	cucwmB239
CsaV3_3G015740	MELO3C006773	Melon (DHL92) v3.5.1	cucmeB236
CsaV3_3G015740	Lsi05G013600	Bottle gourd (USVL1VR-Ls)	cuclsiB245
CsaV3_3G015740	Lsi05G013670	Bottle gourd (USVL1VR-Ls)	cuclsiB229
CsaV3_3G015740	MELO3C006773.2	Melon (DHL92) v3.6.1	cucmedB230
CsaV3_3G015740	Cla97C05G088730	Watermelon (97103) v2	cucwmbB206
CsaV3_3G015740	Bhi01G000843	Wax gourd	cucwgoB272

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_3G015740	Cucurbita maxima (Rimu)	cmacucB0965
CsaV3_3G015740	Watermelon (Charleston Gray)	cucwcgB225