Cla021850 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla021850
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Rapid alkalinization factor 1 (AHRD V1 ***- Q6TF29_SOLCH); contains Interpro domain(s) IPR008801 Rapid ALkalinization Factor
Location	Chr5 : 6857086 .. 6857292 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGATGGAGTCGGAAATAAGTCAAAGGTTTCTTGAACAACAAAGGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGCGATGGCAGCGGTGGCGGTCAACCATACACAAAAAGCGGAAGCTGCGCTCCGCCGCCAACCAATCCTTACAATCGAGGCTGCTCGAAGATATATCGTTGTAGGTCTGATGATTGA

mRNA sequence

ATGCTGATGGAGTCGGAAATAAGTCAAAGGTTTCTTGAACAACAAAGGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGCGATGGCAGCGGTGGCGGTCAACCATACACAAAAAGCGGAAGCTGCGCTCCGCCGCCAACCAATCCTTACAATCGAGGCTGCTCGAAGATATATCGTTGTAGGTCTGATGATTGA

Coding sequence (CDS)

ATGCTGATGGAGTCGGAAATAAGTCAAAGGTTTCTTGAACAACAAAGGAAATACATCTCCATTGGAGCTTTGAAGAAGGATCATCCAGCTTGCGATGGCAGCGGTGGCGGTCAACCATACACAAAAAGCGGAAGCTGCGCTCCGCCGCCAACCAATCCTTACAATCGAGGCTGCTCGAAGATATATCGTTGTAGGTCTGATGATTGA

Protein sequence

MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSKIYRCRSDD

Homology

[Close]

BLAST of Cla021850 vs. Swiss-Prot
Match: RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 6.7e-14
Identity = 36/67 (53.73%), Postives = 44/67 (65.67%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           ++MES  SQR  E+Q   +S GAL+++ PACDG   G+ Y  S  C PPP+NPY+RGCSK
Sbjct: 52  VIMESWSSQRLTEEQAHKLSYGALRRNQPACDGGKRGESY--STQCLPPPSNPYSRGCSK 111

Query: 61  IYRCRSD 68
            YRC  D
Sbjct: 112 HYRCGRD 116

[Close]

BLAST of Cla021850 vs. TrEMBL
Match: A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 2.7e-30
Identity = 61/68 (89.71%), Postives = 66/68 (97.06%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           MLMESEI++RFLEQQ+KYISIGALKKDHPACDG+ GGQPYT+SGSCAPPP NPYNRGCSK
Sbjct: 61  MLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSK 120

Query: 61  IYRCRSDD 69
           IYRCRSDD
Sbjct: 121 IYRCRSDD 128

[Close]

BLAST of Cla021850 vs. TrEMBL
Match: C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 6.7e-21
Identity = 46/67 (68.66%), Postives = 56/67 (83.58%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           +LMESEIS+RFLEQ+R YIS GAL++D P C+G G G+ Y+K+G C PPP+NP NRGCSK
Sbjct: 52  LLMESEISRRFLEQKRSYISNGALQRDKPVCNGGGSGEAYSKTGGCLPPPSNPQNRGCSK 111

Query: 61  IYRCRSD 68
            YRCRSD
Sbjct: 112 YYRCRSD 118

[Close]

BLAST of Cla021850 vs. TrEMBL
Match: A0A103YFY9_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_013276 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.9e-20
Identity = 47/65 (72.31%), Postives = 56/65 (86.15%), Query Frame = 1


Query: 2   LMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSKI 61
           L ESEI++R+LE++R YIS+GALK+D PAC G G G+ YTKSGSC PPP+NPYNRGCSK 
Sbjct: 50  LWESEITRRYLEEKR-YISVGALKRDQPACGGGGRGEAYTKSGSCTPPPSNPYNRGCSKY 109

Query: 62  YRCRS 67
           YRCRS
Sbjct: 110 YRCRS 113

[Close]

BLAST of Cla021850 vs. TrEMBL
Match: B7FMN1_MEDTR (RALF OS=Medicago truncatula GN=MTR_8g083150 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.9e-20
Identity = 48/66 (72.73%), Postives = 55/66 (83.33%), Query Frame = 1


Query: 2   LMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSKI 61
           LMESEIS+RFLEQ R+YIS GALK+D P C+G  GG+ Y+KS  C PPP+NPYNRGCSK 
Sbjct: 54  LMESEISRRFLEQ-RRYISEGALKRDKPVCNGGAGGEAYSKSAGCIPPPSNPYNRGCSKY 113

Query: 62  YRCRSD 68
           YRCRSD
Sbjct: 114 YRCRSD 118

[Close]

BLAST of Cla021850 vs. TrEMBL
Match: A0A0D2UTP2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G152200 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.9e-20
Identity = 49/67 (73.13%), Postives = 55/67 (82.09%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           MLMESEIS+RFLEQ+R YIS GALK+D P C G  GG+ Y+KSG C P P+NPYNRGCSK
Sbjct: 46  MLMESEISRRFLEQKR-YISPGALKRDQPVCRGGAGGEAYSKSGGCLPEPSNPYNRGCSK 105

Query: 61  IYRCRSD 68
            YRCRSD
Sbjct: 106 YYRCRSD 111

[Close]

BLAST of Cla021850 vs. NCBI nr
Match: gi|778679218|ref|XP_004147646.2| (PREDICTED: protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 138.7 bits (348), Expect = 3.9e-30
Identity = 61/68 (89.71%), Postives = 66/68 (97.06%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           MLMESEI++RFLEQQ+KYISIGALKKDHPACDG+ GGQPYT+SGSCAPPP NPYNRGCSK
Sbjct: 61  MLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSK 120

Query: 61  IYRCRSDD 69
           IYRCRSDD
Sbjct: 121 IYRCRSDD 128

[Close]

BLAST of Cla021850 vs. NCBI nr
Match: gi|659077106|ref|XP_008439036.1| (PREDICTED: protein RALF-like 32 [Cucumis melo])

HSP 1 Score: 131.7 bits (330), Expect = 4.8e-28
Identity = 58/68 (85.29%), Postives = 64/68 (94.12%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           +LMESEIS+RFL QQ+KYISIGALKKDHPACDG+ GG+PYTKSGSCAPPP NPY+RGC K
Sbjct: 53  ILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPK 112

Query: 61  IYRCRSDD 69
           IYRCRSDD
Sbjct: 113 IYRCRSDD 120

[Close]

BLAST of Cla021850 vs. NCBI nr
Match: gi|351722809|ref|NP_001235977.1| (uncharacterized protein LOC100500295 precursor [Glycine max])

HSP 1 Score: 107.5 bits (267), Expect = 9.6e-21
Identity = 46/67 (68.66%), Postives = 56/67 (83.58%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           +LMESEIS+RFLEQ+R YIS GAL++D P C+G G G+ Y+K+G C PPP+NP NRGCSK
Sbjct: 52  LLMESEISRRFLEQKRSYISNGALQRDKPVCNGGGSGEAYSKTGGCLPPPSNPQNRGCSK 111

Query: 61  IYRCRSD 68
            YRCRSD
Sbjct: 112 YYRCRSD 118

[Close]

BLAST of Cla021850 vs. NCBI nr
Match: gi|698576934|ref|XP_009776339.1| (PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-20
Identity = 46/67 (68.66%), Postives = 58/67 (86.57%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           +LMES+IS+RFLE+++KYIS GALK+D P C+G GGG+PY++  SC PPP+NPYNRGCSK
Sbjct: 45  ILMESDISRRFLEERKKYISPGALKRDQPVCNGGGGGEPYSR--SCLPPPSNPYNRGCSK 104

Query: 61  IYRCRSD 68
            YRCR D
Sbjct: 105 YYRCRGD 109

[Close]

BLAST of Cla021850 vs. NCBI nr
Match: gi|697103835|ref|XP_009605720.1| (PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis])

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-20
Identity = 46/67 (68.66%), Postives = 58/67 (86.57%), Query Frame = 1


Query: 1   MLMESEISQRFLEQQRKYISIGALKKDHPACDGSGGGQPYTKSGSCAPPPTNPYNRGCSK 60
           +LMES+IS+RFLE+++KYIS GALK+D P C+G GGG+PY++  SC PPP+NPYNRGCSK
Sbjct: 45  ILMESDISRRFLEERKKYISPGALKRDQPVCNGGGGGEPYSR--SCLPPPSNPYNRGCSK 104

Query: 61  IYRCRSD 68
            YRCR D
Sbjct: 105 YYRCRGD 109

The following BLAST results are available for this feature:

BLAST of Cla021850 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF32_ARATH	6.7e-14	53.73	Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1	[more]

BLAST of Cla021850 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9A8_CUCSA	2.7e-30	89.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1	[more]
C6T1A5_SOYBN	6.7e-21	68.66	Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1	[more]
A0A103YFY9_CYNCS	1.9e-20	72.31	Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_013276 PE=4 ...	[more]
B7FMN1_MEDTR	1.9e-20	72.73	RALF OS=Medicago truncatula GN=MTR_8g083150 PE=2 SV=1	[more]
A0A0D2UTP2_GOSRA	1.9e-20	73.13	Uncharacterized protein OS=Gossypium raimondii GN=B456_011G152200 PE=4 SV=1	[more]

BLAST of Cla021850 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679218\|ref\|XP_004147646.2\|	3.9e-30	89.71	PREDICTED: protein RALF-like 32 [Cucumis sativus]	[more]
gi\|659077106\|ref\|XP_008439036.1\|	4.8e-28	85.29	PREDICTED: protein RALF-like 32 [Cucumis melo]	[more]
gi\|351722809\|ref\|NP_001235977.1\|	9.6e-21	68.66	uncharacterized protein LOC100500295 precursor [Glycine max]	[more]
gi\|698576934\|ref\|XP_009776339.1\|	1.3e-20	68.66	PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris]	[more]
gi\|697103835\|ref\|XP_009605720.1\|	1.3e-20	68.66	PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0008150	biological_process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0008430	selenium binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU16186	watermelon unigene v2 vs TrEMBL	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla021850	Cla021850.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU16186	WMU16186	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 1..65 score: 1.4
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 1..68 score: 4.6
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 1..68 score: 4.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla021850	Cla97C05G088730	Watermelon (97103) v2	wmwmbB206
Cla021850	Csa3G171840	Cucumber (Chinese Long) v2	cuwmB233
Cla021850	MELO3C006773	Melon (DHL92) v3.5.1	mewmB461
Cla021850	CSPI03G15560	Wild cucumber (PI 183967)	cpiwmB231
Cla021850	Cucsa.251990	Cucumber (Gy14) v1	cgywmB479
Cla021850	CmaCh14G015430	Cucurbita maxima (Rimu)	cmawmB242
Cla021850	CmoCh06G015950	Cucurbita moschata (Rifu)	cmowmB793
Cla021850	CmoCh14G015780	Cucurbita moschata (Rifu)	cmowmB231
Cla021850	Lsi05G013670	Bottle gourd (USVL1VR-Ls)	lsiwmB366
Cla021850	Cp4.1LG03g12150	Cucurbita pepo (Zucchini)	cpewmB613
Cla021850	Cp4.1LG08g05550	Cucurbita pepo (Zucchini)	cpewmB845
Cla021850	CsGy3G015610	Cucumber (Gy14) v2	cgybwmB217
Cla021850	MELO3C006773.2	Melon (DHL92) v3.6.1	medwmB447
Cla021850	Carg15515	Silver-seed gourd	carwmB0692
Cla021850	Carg24781	Silver-seed gourd	carwmB1043
Cla021850	CsaV3_3G015740	Cucumber (Chinese Long) v3	cucwmB239
Cla021850	Bhi01G000843	Wax gourd	wgowmB201

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla021850	Cucurbita maxima (Rimu)	cmawmB798
Cla021850	Watermelon (Charleston Gray)	wcgwmB286