CmoCh06G015950 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh06G015950
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	RALF
Location	Cmo_Chr06 : 11175436 .. 11175696 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATGAGTTCTTGCAATGTCTCAATGACCGAGCATGGTTATGAAGATGAGCTGTTAGTGCAATCTGAGATGGGTAGAAGGTTTCTCGAAGAACAAAAGAAATATATCTCCATTGAAGCTTTAAAGAAGGATATGATAGCTTTTGAGGGTAGCAGCGGAGGCCAACCTTACCCCAAAAGCGAAAACTGTGCTCCCCCACCTCTTAATCCGTATAACCGAGGCTGCTCTAAAATATCTCGTTGTAGGTCTGATGATTGA

mRNA sequence

ATGTCGATGAGTTCTTGCAATGTCTCAATGACCGAGCATGGTTATGAAGATGAGCTGTTAGTGCAATCTGAGATGGGTAGAAGGTTTCTCGAAGAACAAAAGAAATATATCTCCATTGAAGCTTTAAAGAAGGATATGATAGCTTTTGAGGGTAGCAGCGGAGGCCAACCTTACCCCAAAAGCGAAAACTGTGCTCCCCCACCTCTTAATCCGTATAACCGAGGCTGCTCTAAAATATCTCGTTGTAGGTCTGATGATTGA

Coding sequence (CDS)

ATGTCGATGAGTTCTTGCAATGTCTCAATGACCGAGCATGGTTATGAAGATGAGCTGTTAGTGCAATCTGAGATGGGTAGAAGGTTTCTCGAAGAACAAAAGAAATATATCTCCATTGAAGCTTTAAAGAAGGATATGATAGCTTTTGAGGGTAGCAGCGGAGGCCAACCTTACCCCAAAAGCGAAAACTGTGCTCCCCCACCTCTTAATCCGTATAACCGAGGCTGCTCTAAAATATCTCGTTGTAGGTCTGATGATTGA

Homology

[Close]

BLAST of CmoCh06G015950 vs. Swiss-Prot
Match: RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.3e-08
Identity = 35/89 (39.33%), Postives = 48/89 (53.93%), Query Frame = 1


Query: 2   SMSSCNVSMTE-----HGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQ 61
           S S CN S+ E        E  ++++S   +R  EEQ   +S  AL+++  A +G   G+
Sbjct: 30  SSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACDGGKRGE 89

Query: 62  PYPKSENCAPPPLNPYNRGCSKISRCRSD 86
            Y  S  C PPP NPY+RGCSK  RC  D
Sbjct: 90  SY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of CmoCh06G015950 vs. TrEMBL
Match: A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-23
Identity = 57/85 (67.06%), Postives = 68/85 (80.00%), Query Frame = 1


Query: 2   SMSSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKS 61
           S  SCN S+     E+E+L++SE+ RRFLE+QKKYISI ALKKD  A +G+SGGQPY +S
Sbjct: 44  STHSCNGSIAACANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRS 103

Query: 62  ENCAPPPLNPYNRGCSKISRCRSDD 87
            +CAPPP NPYNRGCSKI RCRSDD
Sbjct: 104 GSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of CmoCh06G015950 vs. TrEMBL
Match: I3RZ93_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.3e-17
Identity = 48/82 (58.54%), Postives = 58/82 (70.73%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S+CN S+ E   EDELL++SE+ RRFLEE ++YIS  ALK+D     G + G+ Y KS  
Sbjct: 32  STCNGSIAECNQEDELLMESEISRRFLEE-RRYISPGALKRDKPVCNGGASGEAYSKSGG 91

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NPYNRGCSK  RCRSD
Sbjct: 92  CLPPPSNPYNRGCSKYYRCRSD 112

[Close]

BLAST of CmoCh06G015950 vs. TrEMBL
Match: B7FMN1_MEDTR (RALF OS=Medicago truncatula GN=MTR_8g083150 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.4e-17
Identity = 48/82 (58.54%), Postives = 57/82 (69.51%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S CN S+ E   EDE L++SE+ RRFLE Q++YIS  ALK+D     G +GG+ Y KS  
Sbjct: 38  SICNGSIAECNQEDEQLMESEISRRFLE-QRRYISEGALKRDKPVCNGGAGGEAYSKSAG 97

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NPYNRGCSK  RCRSD
Sbjct: 98  CIPPPSNPYNRGCSKYYRCRSD 118

[Close]

BLAST of CmoCh06G015950 vs. TrEMBL
Match: C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.8e-16
Identity = 44/82 (53.66%), Postives = 56/82 (68.29%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S+CN S+ E   EDELL++SE+ RRFLE+++ YIS  AL++D     G   G+ Y K+  
Sbjct: 37  STCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQRDKPVCNGGGSGEAYSKTGG 96

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NP NRGCSK  RCRSD
Sbjct: 97  CLPPPSNPQNRGCSKYYRCRSD 118

[Close]

BLAST of CmoCh06G015950 vs. TrEMBL
Match: A0A0S3R1D3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.8e-16
Identity = 47/83 (56.63%), Postives = 56/83 (67.47%), Query Frame = 1


Query: 3   MSSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSE 62
           +S+CN S+ E   EDELL++SE  RRFLE QKKYIS  AL++D     G   G+ Y  S 
Sbjct: 36  VSTCNGSIAECNQEDELLMESETSRRFLE-QKKYISNGALQRDKPVCNGGGSGEAYSNSG 95

Query: 63  NCAPPPLNPYNRGCSKISRCRSD 86
            C PPP NP+NRGCSK  RCRSD
Sbjct: 96  GCLPPPSNPHNRGCSKYYRCRSD 117

[Close]

BLAST of CmoCh06G015950 vs. TAIR10
Match: AT4G14010.1 (AT4G14010.1 ralf-like 32)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-09
Identity = 35/89 (39.33%), Postives = 48/89 (53.93%), Query Frame = 1


Query: 2   SMSSCNVSMTE-----HGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQ 61
           S S CN S+ E        E  ++++S   +R  EEQ   +S  AL+++  A +G   G+
Sbjct: 30  SSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACDGGKRGE 89

Query: 62  PYPKSENCAPPPLNPYNRGCSKISRCRSD 86
            Y  S  C PPP NPY+RGCSK  RC  D
Sbjct: 90  SY--STQCLPPPSNPYSRGCSKHYRCGRD 116

[Close]

BLAST of CmoCh06G015950 vs. TAIR10
Match: AT4G13950.1 (AT4G13950.1 ralf-like 31)

HSP 1 Score: 50.4 bits (119), Expect = 6.2e-07
Identity = 27/70 (38.57%), Postives = 39/70 (55.71%), Query Frame = 1


Query: 16  EDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSENCAPPPLNPYNRG 75
           EDE L+ +E+ RR L  QK+YI  E L++DM+  +     +P     +C     N Y+RG
Sbjct: 43  EDEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQ-----KPGASYYDCRSGQANSYSRG 102

Query: 76  CSKISRCRSD 86
           C  I+RC  D
Sbjct: 103 CDTITRCARD 107

[Close]

BLAST of CmoCh06G015950 vs. TAIR10
Match: AT3G23805.1 (AT3G23805.1 ralf-like 24)

HSP 1 Score: 48.9 bits (115), Expect = 1.8e-06
Identity = 27/70 (38.57%), Postives = 39/70 (55.71%), Query Frame = 1


Query: 16  EDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSENCAPPPLNPYNRG 75
           E E ++ SE+ RR +  +K+YIS E L++DM+  +     +P      C     N YNRG
Sbjct: 48  EGEEMMPSEISRRVMMMRKQYISYETLRRDMVPCQ-----KPGASYYACRSGQANAYNRG 107

Query: 76  CSKISRCRSD 86
           CS I+RC  D
Sbjct: 108 CSVITRCARD 112

[Close]

BLAST of CmoCh06G015950 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 48.9 bits (115), Expect = 1.8e-06
Identity = 34/81 (41.98%), Postives = 47/81 (58.02%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S C+ S+ E    +E  + SE+ RR L   K YIS ++LK++ +    S  G  Y   +N
Sbjct: 45  SGCHGSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPC--SRRGASYYNCQN 104

Query: 64  CAPPPLNPYNRGCSKISRCRS 85
            A    NPY+RGCSKI+RCRS
Sbjct: 105 GAQA--NPYSRGCSKIARCRS 120

[Close]

BLAST of CmoCh06G015950 vs. NCBI nr
Match: gi|659077106|ref|XP_008439036.1| (PREDICTED: protein RALF-like 32 [Cucumis melo])

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-23
Identity = 57/85 (67.06%), Postives = 68/85 (80.00%), Query Frame = 1


Query: 2   SMSSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKS 61
           S  SCN S+ E G EDE+L++SE+ RRFL +QKKYISI ALKKD  A +G+SGG+PY KS
Sbjct: 36  STHSCNGSIAECGSEDEILMESEISRRFLAQQKKYISIGALKKDHPACDGASGGRPYTKS 95

Query: 62  ENCAPPPLNPYNRGCSKISRCRSDD 87
            +CAPPP NPY+RGC KI RCRSDD
Sbjct: 96  GSCAPPPANPYDRGCPKIYRCRSDD 120

[Close]

BLAST of CmoCh06G015950 vs. NCBI nr
Match: gi|778679218|ref|XP_004147646.2| (PREDICTED: protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-23
Identity = 57/85 (67.06%), Postives = 68/85 (80.00%), Query Frame = 1


Query: 2   SMSSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKS 61
           S  SCN S+     E+E+L++SE+ RRFLE+QKKYISI ALKKD  A +G+SGGQPY +S
Sbjct: 44  STHSCNGSIAACANEEEMLMESEITRRFLEQQKKYISIGALKKDHPACDGASGGQPYTRS 103

Query: 62  ENCAPPPLNPYNRGCSKISRCRSDD 87
            +CAPPP NPYNRGCSKI RCRSDD
Sbjct: 104 GSCAPPPANPYNRGCSKIYRCRSDD 128

[Close]

BLAST of CmoCh06G015950 vs. NCBI nr
Match: gi|388490538|gb|AFK33335.1| (unknown [Lotus japonicus])

HSP 1 Score: 95.5 bits (236), Expect = 4.8e-17
Identity = 48/82 (58.54%), Postives = 58/82 (70.73%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S+CN S+ E   EDELL++SE+ RRFLEE ++YIS  ALK+D     G + G+ Y KS  
Sbjct: 32  STCNGSIAECNQEDELLMESEISRRFLEE-RRYISPGALKRDKPVCNGGASGEAYSKSGG 91

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NPYNRGCSK  RCRSD
Sbjct: 92  CLPPPSNPYNRGCSKYYRCRSD 112

[Close]

BLAST of CmoCh06G015950 vs. NCBI nr
Match: gi|357518655|ref|XP_003629616.1| (RALF [Medicago truncatula])

HSP 1 Score: 95.1 bits (235), Expect = 6.2e-17
Identity = 48/82 (58.54%), Postives = 57/82 (69.51%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S CN S+ E   EDE L++SE+ RRFLE Q++YIS  ALK+D     G +GG+ Y KS  
Sbjct: 38  SICNGSIAECNQEDEQLMESEISRRFLE-QRRYISEGALKRDKPVCNGGAGGEAYSKSAG 97

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NPYNRGCSK  RCRSD
Sbjct: 98  CIPPPSNPYNRGCSKYYRCRSD 118

[Close]

BLAST of CmoCh06G015950 vs. NCBI nr
Match: gi|698576934|ref|XP_009776339.1| (PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-16
Identity = 46/82 (56.10%), Postives = 57/82 (69.51%), Query Frame = 1


Query: 4   SSCNVSMTEHGYEDELLVQSEMGRRFLEEQKKYISIEALKKDMIAFEGSSGGQPYPKSEN 63
           S CN ++ E   E E+L++S++ RRFLEE+KKYIS  ALK+D     G  GG+PY  S +
Sbjct: 30  SPCNGTIAECNEELEILMESDISRRFLEERKKYISPGALKRDQPVCNGGGGGEPY--SRS 89

Query: 64  CAPPPLNPYNRGCSKISRCRSD 86
           C PPP NPYNRGCSK  RCR D
Sbjct: 90  CLPPPSNPYNRGCSKYYRCRGD 109

The following BLAST results are available for this feature:

BLAST of CmoCh06G015950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF32_ARATH	5.3e-08	39.33	Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1	[more]

BLAST of CmoCh06G015950 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9A8_CUCSA	1.1e-23	67.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1	[more]
I3RZ93_LOTJA	3.3e-17	58.54	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
B7FMN1_MEDTR	4.4e-17	58.54	RALF OS=Medicago truncatula GN=MTR_8g083150 PE=2 SV=1	[more]
C6T1A5_SOYBN	2.8e-16	53.66	Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1	[more]
A0A0S3R1D3_PHAAN	4.8e-16	56.63	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=...	[more]

BLAST of CmoCh06G015950 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14010.1	3.0e-09	39.33	ralf-like 32	[more]
AT4G13950.1	6.2e-07	38.57	ralf-like 31	[more]
AT3G23805.1	1.8e-06	38.57	ralf-like 24	[more]
AT1G02900.1	1.8e-06	41.98	rapid alkalinization factor 1	[more]

BLAST of CmoCh06G015950 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077106\|ref\|XP_008439036.1\|	1.2e-23	67.06	PREDICTED: protein RALF-like 32 [Cucumis melo]	[more]
gi\|778679218\|ref\|XP_004147646.2\|	1.5e-23	67.06	PREDICTED: protein RALF-like 32 [Cucumis sativus]	[more]
gi\|388490538\|gb\|AFK33335.1\|	4.8e-17	58.54	unknown [Lotus japonicus]	[more]
gi\|357518655\|ref\|XP_003629616.1\|	6.2e-17	58.54	RALF [Medicago truncatula]	[more]
gi\|698576934\|ref\|XP_009776339.1\|	1.8e-16	56.10	PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G015950.1	CmoCh06G015950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 18..83 score: 1.1
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 12..86 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh06G015950	Cucsa.251990	Cucumber (Gy14) v1	cgycmoB0718
CmoCh06G015950	CmaCh14G015430	Cucurbita maxima (Rimu)	cmacmoB271
CmoCh06G015950	Cla021850	Watermelon (97103) v1	cmowmB793
CmoCh06G015950	Csa3G171840	Cucumber (Chinese Long) v2	cmocuB806
CmoCh06G015950	MELO3C006773	Melon (DHL92) v3.5.1	cmomeB755
CmoCh06G015950	CSPI03G15560	Wild cucumber (PI 183967)	cmocpiB814
CmoCh06G015950	Lsi05G013670	Bottle gourd (USVL1VR-Ls)	cmolsiB759
CmoCh06G015950	Cp4.1LG08g05550	Cucurbita pepo (Zucchini)	cmocpeB796
CmoCh06G015950	Cp4.1LG03g12150	Cucurbita pepo (Zucchini)	cmocpeB787
CmoCh06G015950	MELO3C006773.2	Melon (DHL92) v3.6.1	cmomedB857
CmoCh06G015950	CsaV3_3G015740	Cucumber (Chinese Long) v3	cmocucB0953
CmoCh06G015950	Cla97C05G088730	Watermelon (97103) v2	cmowmbB833
CmoCh06G015950	Bhi01G000843	Wax gourd	cmowgoB1005
CmoCh06G015950	CsGy3G015610	Cucumber (Gy14) v2	cgybcmoB401
CmoCh06G015950	Carg24781	Silver-seed gourd	carcmoB1394
CmoCh06G015950	Carg15515	Silver-seed gourd	carcmoB0947

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh06G015950	CmoCh14G015780	Cucurbita moschata (Rifu)	cmocmoB224

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh06G015950	Cucurbita maxima (Rimu)	cmacmoB829
CmoCh06G015950	Watermelon (Charleston Gray)	cmowcgB743