Cp4.1LG03g12260 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG03g12260
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Unknown protein
Location	Cp4.1LG03 : 10438792 .. 10439130 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCATCATCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTTGATAAGCCCGTGCAATGGTTCAATAGCGGAGTGCTATAAAGAAGTTGAGACGTTGATGGAGTCAGAGATAAGTCGGAGGTTTCTCGGACGACATAAGGATTTAACCTATGATTTTTTGGATAAAAACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACCGTGGAAAGTGTCAGCCTCGTGAAGCTAATCCTTATGACAGAGAATGCAGCGGAACGTATAATCGATGTAAATAG

mRNA sequence

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCATCATCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTTGATAAGCCCGTGCAATGGTTCAATAGCGGAGTGCTATAAAGAAGTTGAGACGTTGATGGAGTCAGAGATAAGTCGGAGGTTTCTCGGACGACATAAGGATTTAACCTATGATTTTTTGGATAAAAACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACCGTGGAAAGTGTCAGCCTCGTGAAGCTAATCCTTATGACAGAGAATGCAGCGGAACGTATAATCGATGTAAATAG

Coding sequence (CDS)

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCATCATCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTTGATAAGCCCGTGCAATGGTTCAATAGCGGAGTGCTATAAAGAAGTTGAGACGTTGATGGAGTCAGAGATAAGTCGGAGGTTTCTCGGACGACATAAGGATTTAACCTATGATTTTTTGGATAAAAACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACCGTGGAAAGTGTCAGCCTCGTGAAGCTAATCCTTATGACAGAGAATGCAGCGGAACGTATAATCGATGTAAATAG

Protein sequence

MEGSSSHSSLNLIIGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGKCQPREANPYDRECSGTYNRCK

Homology

[Close]

BLAST of Cp4.1LG03g12260 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-09
Identity = 36/81 (44.44%), Postives = 48/81 (59.26%), Query Frame = 1


Query: 33  SLISPCNGSIAECYKEVETL-MESEISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGK 92
           SL S C GSIAEC  E E +  +S+ISRR L + K ++Y  + +N  PC+  RG  Y   
Sbjct: 40  SLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSR-RGASYYNC 99

Query: 93  CQPREANPYDRECSGTYNRCK 113
            +  +ANPY R CS T  RC+
Sbjct: 100 QRGAQANPYSRGCS-TITRCR 118

[Close]

BLAST of Cp4.1LG03g12260 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-08
Identity = 32/70 (45.71%), Postives = 40/70 (57.14%), Query Frame = 1


Query: 36  SPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGKCQPR 95
           S C+GSIAEC    E  M+SEI+RR L   K ++Y  L +N  PC+  RG  Y       
Sbjct: 45  SGCHGSIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSR-RGASYYNCQNGA 104

Query: 96  EANPYDRECS 106
           +ANPY R CS
Sbjct: 105 QANPYSRGCS 113

[Close]

BLAST of Cp4.1LG03g12260 vs. TrEMBL
Match: A0A0S3R1D3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.0e-09
Identity = 44/103 (42.72%), Postives = 63/103 (61.17%), Query Frame = 1


Query: 17  LYLLIFT----NVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDF 76
           +YL+ F+    +   V+++ S +S CNGSIAEC +E E LMESE SRRFL + K ++   
Sbjct: 14  IYLIFFSFMRLSSCTVLSLTSHVSTCNGSIAECNQEDELLMESETSRRFLEQKKYISNGA 73

Query: 77  LDKNKAPC-ANGRGKPY--RGKCQPREANPYDRECSGTYNRCK 113
           L ++K  C   G G+ Y   G C P  +NP++R CS  Y RC+
Sbjct: 74  LQRDKPVCNGGGSGEAYSNSGGCLPPPSNPHNRGCS-KYYRCR 115

[Close]

BLAST of Cp4.1LG03g12260 vs. TrEMBL
Match: A0A151SJC7_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_001065 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 7.4e-09
Identity = 40/88 (45.45%), Postives = 55/88 (62.50%), Query Frame = 1


Query: 28  VVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDKNKAPC-ANGRGK 87
           V+++ S  S CNGSI+EC +E E LMESEISRRFL + + ++   L ++K  C   G G+
Sbjct: 8   VISLTSHASTCNGSISECNQEDELLMESEISRRFLEQKRYISNGALQRDKPVCNGGGSGE 67

Query: 88  PY--RGKCQPREANPYDRECSGTYNRCK 113
            Y   G C P  +NP +R CS  Y RC+
Sbjct: 68  AYSKTGGCLPPPSNPQNRGCS-KYYRCR 94

[Close]

BLAST of Cp4.1LG03g12260 vs. TrEMBL
Match: A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-08
Identity = 48/106 (45.28%), Postives = 64/106 (60.38%), Query Frame = 1


Query: 12  LIIGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKD-LTY 71
           L++  L+L +F ++ +V A  S    CNGSIA C  E E LMESEI+RRFL + K  ++ 
Sbjct: 24  LLLLFLFLFLFQDLCLVRA--SSTHSCNGSIAACANEEEMLMESEITRRFLEQQKKYISI 83

Query: 72  DFLDKNKAPC--ANGRGKPY--RGKCQPREANPYDRECSGTYNRCK 113
             L K+   C  A+G G+PY   G C P  ANPY+R CS  Y RC+
Sbjct: 84  GALKKDHPACDGASG-GQPYTRSGSCAPPPANPYNRGCSKIY-RCR 125

[Close]

BLAST of Cp4.1LG03g12260 vs. TrEMBL
Match: W9RGL7_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_026861 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-08
Identity = 41/99 (41.41%), Postives = 57/99 (57.58%), Query Frame = 1


Query: 16  LLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDK 75
           +L LL F++  ++       +PCNGSIA+C  E E LMESEISRR L + K +T   L  
Sbjct: 12  ILSLLYFSSASLIKGYT--YTPCNGSIADCDVEGEMLMESEISRRVLAQRKYITPGALKP 71

Query: 76  NKAPCANGRGKPY--RGKCQPREANPYDRECSGTYNRCK 113
           ++  C   +G+ Y   G C P ++NP DR C   Y RC+
Sbjct: 72  DQPVCKGAKGEAYSKAGDCLPEQSNPPDRGCQ-KYYRCR 107

[Close]

BLAST of Cp4.1LG03g12260 vs. TrEMBL
Match: A0A0A0L624_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171850 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-08
Identity = 42/97 (43.30%), Postives = 54/97 (55.67%), Query Frame = 1


Query: 9   SLNLIIGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKD- 68
           SL     LL  L F    + +   S +  CNGSIAEC  E E LMESEI+RRFL + K  
Sbjct: 2   SLQTCFLLLLFLSFLLQDLCLVRGSSMHSCNGSIAECANEEEMLMESEITRRFLAQQKKN 61

Query: 69  -LTYDFLDKNKAPCANGRGKPY--RGKCQPREANPYD 102
            ++Y  L K++  C    G+PY   G C P +ANPY+
Sbjct: 62  YISYPTLKKDRPACDGASGQPYTKSGSCVPSQANPYN 98

[Close]

BLAST of Cp4.1LG03g12260 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-10
Identity = 36/81 (44.44%), Postives = 48/81 (59.26%), Query Frame = 1


Query: 33  SLISPCNGSIAECYKEVETL-MESEISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGK 92
           SL S C GSIAEC  E E +  +S+ISRR L + K ++Y  + +N  PC+  RG  Y   
Sbjct: 40  SLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSR-RGASYYNC 99

Query: 93  CQPREANPYDRECSGTYNRCK 113
            +  +ANPY R CS T  RC+
Sbjct: 100 QRGAQANPYSRGCS-TITRCR 118

[Close]

BLAST of Cp4.1LG03g12260 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-09
Identity = 32/70 (45.71%), Postives = 40/70 (57.14%), Query Frame = 1


Query: 36  SPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGKCQPR 95
           S C+GSIAEC    E  M+SEI+RR L   K ++Y  L +N  PC+  RG  Y       
Sbjct: 45  SGCHGSIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSR-RGASYYNCQNGA 104

Query: 96  EANPYDRECS 106
           +ANPY R CS
Sbjct: 105 QANPYSRGCS 113

[Close]

BLAST of Cp4.1LG03g12260 vs. NCBI nr
Match: gi|720097363|ref|XP_010247297.1| (PREDICTED: protein RALF-like 32 [Nelumbo nucifera])

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-13
Identity = 52/116 (44.83%), Postives = 66/116 (56.90%), Query Frame = 1


Query: 1   MEGSSSHSSLNLIIGLLYLL--IFTNVVVVVAVDSL--ISPCNGSIAECYKEVETLMESE 60
           M  SS+ SSL  I+  L LL  +++  V V A +S      CNGSIAEC +E E LMESE
Sbjct: 39  MAASSNRSSLGFILATLLLLQLLYSGCVRVSAAESSERYRQCNGSIAECSEEEEVLMESE 98

Query: 61  ISRRFLGRHKDLTYDFLDKNKAPCANGRGKPYRGKCQPREANPYDRECSGTYNRCK 113
           ISRRFL +   + Y  L  N+  C   +G  Y G C P  +NP +R CS  Y RC+
Sbjct: 99  ISRRFLAQSNSIGYGALKPNQPACGGPKGTSYSGSCLPNPSNPQNRGCSKIY-RCR 153

[Close]

BLAST of Cp4.1LG03g12260 vs. NCBI nr
Match: gi|697128698|ref|XP_009618400.1| (PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis])

HSP 1 Score: 75.1 bits (183), Expect = 8.7e-11
Identity = 46/112 (41.07%), Postives = 71/112 (63.39%), Query Frame = 1


Query: 3   GSSSHSSLNLIIGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFL 62
           GSS+ ++L     + +  IF  ++ ++   +  SPCNG+IAEC +E+E LMES+ISRRFL
Sbjct: 24  GSSNFTTL-----IHFFTIFLTLLFIITSVTGQSPCNGTIAECNEELEILMESDISRRFL 83

Query: 63  -GRHKDLTYDFLDKNKAPC-ANGRGKPYRGKCQPREANPYDRECSGTYNRCK 113
             R K ++   L +++  C + G G+PY  +C P  +NPY+R CS  Y RC+
Sbjct: 84  EERKKYISPGALKRDQPVCNSGGGGEPYSRRCLPPPSNPYNRGCS-KYYRCR 129

[Close]

BLAST of Cp4.1LG03g12260 vs. NCBI nr
Match: gi|698576934|ref|XP_009776339.1| (PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-10
Identity = 44/100 (44.00%), Postives = 59/100 (59.00%), Query Frame = 1


Query: 16  LLYLL-IFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLD 75
           L+Y+  IF  ++ ++   +  SPCNG+IAEC +E+E LMES+ISRRFL   K        
Sbjct: 9   LIYIFSIFLTLLFIITSVTGQSPCNGTIAECNEELEILMESDISRRFLEERKKYISPGAL 68

Query: 76  KNKAPCAN--GRGKPYRGKCQPREANPYDRECSGTYNRCK 113
           K   P  N  G G+PY   C P  +NPY+R CS  Y RC+
Sbjct: 69  KRDQPVCNGGGGGEPYSRSCLPPPSNPYNRGCS-KYYRCR 107

[Close]

BLAST of Cp4.1LG03g12260 vs. NCBI nr
Match: gi|697103835|ref|XP_009605720.1| (PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis])

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-10
Identity = 42/99 (42.42%), Postives = 57/99 (57.58%), Query Frame = 1


Query: 16  LLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDK 75
           L +  +F  ++ ++   +  SPCNG+IAEC +E+E LMES+ISRRFL   K        K
Sbjct: 10  LYFFTLFLTLLFIITSVTGQSPCNGTIAECNEELEILMESDISRRFLEERKKYISPGALK 69

Query: 76  NKAPCAN--GRGKPYRGKCQPREANPYDRECSGTYNRCK 113
              P  N  G G+PY   C P  +NPY+R CS  Y RC+
Sbjct: 70  RDQPVCNGGGGGEPYSRSCLPPPSNPYNRGCS-KYYRCR 107

[Close]

BLAST of Cp4.1LG03g12260 vs. NCBI nr
Match: gi|720097359|ref|XP_010247296.1| (PREDICTED: protein RALF-like 32 [Nelumbo nucifera])

HSP 1 Score: 72.8 bits (177), Expect = 4.3e-10
Identity = 39/77 (50.65%), Postives = 48/77 (62.34%), Query Frame = 1


Query: 37  PCNGSIAECYKEVETLMESEISRRFLGRHKDLTYDFLDKNKAPCANG-RGKPYRGKCQPR 96
           P N SIAEC +E E LMESEISRRFL +   ++Y  L  ++A C NG RGKPY   C P 
Sbjct: 42  PFNDSIAECSREEEGLMESEISRRFLEQTNRISYGALKSDQATCGNGARGKPYSNSCLPT 101

Query: 97  EANPYDRECSGTYNRCK 113
            +N  +R C   Y RC+
Sbjct: 102 PSNTVNRGCFKVY-RCR 117

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g12260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF22_ARATH	2.1e-09	44.44	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]
RLF1_ARATH	1.8e-08	45.71	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]

BLAST of Cp4.1LG03g12260 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0S3R1D3_PHAAN	2.0e-09	42.72	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G214200 PE=...	[more]
A0A151SJC7_CAJCA	7.4e-09	45.45	Uncharacterized protein OS=Cajanus cajan GN=KK1_001065 PE=4 SV=1	[more]
A0A0A0L9A8_CUCSA	2.2e-08	45.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1	[more]
W9RGL7_9ROSA	2.2e-08	41.41	Uncharacterized protein OS=Morus notabilis GN=L484_026861 PE=4 SV=1	[more]
A0A0A0L624_CUCSA	2.8e-08	43.30	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171850 PE=4 SV=1	[more]

BLAST of Cp4.1LG03g12260 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G05490.1	1.2e-10	44.44	ralf-like 22	[more]
AT1G02900.1	1.0e-09	45.71	rapid alkalinization factor 1	[more]

BLAST of Cp4.1LG03g12260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|720097363\|ref\|XP_010247297.1\|	1.1e-13	44.83	PREDICTED: protein RALF-like 32 [Nelumbo nucifera]	[more]
gi\|697128698\|ref\|XP_009618400.1\|	8.7e-11	41.07	PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis]	[more]
gi\|698576934\|ref\|XP_009776339.1\|	2.5e-10	44.00	PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris]	[more]
gi\|697103835\|ref\|XP_009605720.1\|	2.5e-10	42.42	PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis]	[more]
gi\|720097359\|ref\|XP_010247296.1\|	4.3e-10	50.65	PREDICTED: protein RALF-like 32 [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0007154	cell communication
biological_process	GO:0044700	single organism signaling
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g12260.1	Cp4.1LG03g12260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 50..112 score: 5.2
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 16..111 score: 2.1
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 16..111 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG03g12260	Cucsa.251990	Cucumber (Gy14) v1	cgycpeB0682
Cp4.1LG03g12260	CmaCh14G015310	Cucurbita maxima (Rimu)	cmacpeB283
Cp4.1LG03g12260	CmoCh14G015780	Cucurbita moschata (Rifu)	cmocpeB245
Cp4.1LG03g12260	Cla021855	Watermelon (97103) v1	cpewmB613
Cp4.1LG03g12260	Csa3G171840	Cucumber (Chinese Long) v2	cpecuB609
Cp4.1LG03g12260	MELO3C006773	Melon (DHL92) v3.5.1	cpemeB585
Cp4.1LG03g12260	CSPI03G15560	Wild cucumber (PI 183967)	cpecpiB611
Cp4.1LG03g12260	Lsi05G013600	Bottle gourd (USVL1VR-Ls)	cpelsiB507
Cp4.1LG03g12260	MELO3C006773.2	Melon (DHL92) v3.6.1	cpemedB693
Cp4.1LG03g12260	CsaV3_3G015740	Cucumber (Chinese Long) v3	cpecucB0759
Cp4.1LG03g12260	Bhi01G000851	Wax gourd	cpewgoB0783
Cp4.1LG03g12260	CsGy3G015610	Cucumber (Gy14) v2	cgybcpeB406
Cp4.1LG03g12260	Carg24781	Silver-seed gourd	carcpeB1446

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG03g12260	Cucurbita pepo (Zucchini)	cpecpeB482
Cp4.1LG03g12260	Cucurbita maxima (Rimu)	cmacpeB837
Cp4.1LG03g12260	Cucurbita moschata (Rifu)	cmocpeB787
Cp4.1LG03g12260	Bottle gourd (USVL1VR-Ls)	cpelsiB490
Cp4.1LG03g12260	Watermelon (Charleston Gray)	cpewcgB554
Cp4.1LG03g12260	Watermelon (Charleston Gray)	cpewcgB559
Cp4.1LG03g12260	Watermelon (97103) v1	cpewmB619
Cp4.1LG03g12260	Melon (DHL92) v3.6.1	cpemedB701
Cp4.1LG03g12260	Silver-seed gourd	carcpeB0973