Cla021855 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla021855
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Unknown Protein (AHRD V1); contains Interpro domain(s) IPR008801 Rapid ALkalinization Factor
Location	Chr5 : 6938442 .. 6938783 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGAAGCTTTAGCCACCAAAGCCTCGGCGTTGTCCTCGGCCTTCTATATCTATTGATTTTTAGTAACATTGTCGTTATTGCCATTGATAGCTTGACGAGCCCCTGCAATGGTTCGATTGCAGAGTGCTACGAAGAAGAAGAGATGTTAATGGAGTCAGAGATAAGTCGAAGGTTTCTCGAACAAAAGACCAAACACATATCGTATGATGTTTTGATTAAAGACAAACCGACTTGCAATAAAGGTGCAGGCAAACCTTACGGTAAAAATTGTAAGTCTTCTGAAGCTAATCCTTATGATAGAGGATGCGCTGGAATATATAATCGATGTAGGACTTAG

mRNA sequence

ATGAAAGGAAGCTTTAGCCACCAAAGCCTCGGCGTTGTCCTCGGCCTTCTATATCTATTGATTTTTAGTAACATTGTCGTTATTGCCATTGATAGCTTGACGAGCCCCTGCAATGGTTCGATTGCAGAGTGCTACGAAGAAGAAGAGATGTTAATGGAGTCAGAGATAAGTCGAAGGTTTCTCGAACAAAAGACCAAACACATATCGTATGATGTTTTGATTAAAGACAAACCGACTTGCAATAAAGGTGCAGGCAAACCTTACGGTAAAAATTGTAAGTCTTCTGAAGCTAATCCTTATGATAGAGGATGCGCTGGAATATATAATCGATGTAGGACTTAG

Coding sequence (CDS)

ATGAAAGGAAGCTTTAGCCACCAAAGCCTCGGCGTTGTCCTCGGCCTTCTATATCTATTGATTTTTAGTAACATTGTCGTTATTGCCATTGATAGCTTGACGAGCCCCTGCAATGGTTCGATTGCAGAGTGCTACGAAGAAGAAGAGATGTTAATGGAGTCAGAGATAAGTCGAAGGTTTCTCGAACAAAAGACCAAACACATATCGTATGATGTTTTGATTAAAGACAAACCGACTTGCAATAAAGGTGCAGGCAAACCTTACGGTAAAAATTGTAAGTCTTCTGAAGCTAATCCTTATGATAGAGGATGCGCTGGAATATATAATCGATGTAGGACTTAG

Protein sequence

MKGSFSHQSLGVVLGLLYLLIFSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCNKGAGKPYGKNCKSSEANPYDRGCAGIYNRCRT

Homology

[Close]

BLAST of Cla021855 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 3.0e-11
Identity = 42/83 (50.60%), Postives = 52/83 (62.65%), Query Frame = 1


Query: 32  SLTSPCNGSIAECY-EEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCNKGAGKPYGK 91
           SL S C GSIAEC  EEEEM  +S+ISRR L QK K+ISY  + ++   C++     Y  
Sbjct: 40  SLFSGCTGSIAECIAEEEEMEFDSDISRRILAQK-KYISYGAMRRNSVPCSRRGASYY-- 99

Query: 92  NC-KSSEANPYDRGCAGIYNRCR 113
           NC + ++ANPY RGC+ I  RCR
Sbjct: 100 NCQRGAQANPYSRGCSTI-TRCR 118

[Close]

BLAST of Cla021855 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-09
Identity = 38/80 (47.50%), Postives = 50/80 (62.50%), Query Frame = 1


Query: 35  SPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCNKGAGKPYGKNCKS 94
           S C+GSIAEC   EE  M+SEI+RR L   TK+ISY  L ++   C++     Y  NC++
Sbjct: 45  SGCHGSIAECIGAEEEEMDSEINRRIL-ATTKYISYQSLKRNSVPCSRRGASYY--NCQN 104

Query: 95  -SEANPYDRGCAGIYNRCRT 114
            ++ANPY RGC+ I  RCR+
Sbjct: 105 GAQANPYSRGCSKI-ARCRS 120

[Close]

BLAST of Cla021855 vs. Swiss-Prot
Match: RLF32_ARATH (Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-09
Identity = 35/86 (40.70%), Postives = 48/86 (55.81%), Query Frame = 1


Query: 32  SLTSPCNGSIAECY-----EEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCNKG-AG 91
           S +S CNGS+AEC      EE  ++MES  S+R  E++   +SY  L +++P C+ G  G
Sbjct: 29  SSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACDGGKRG 88

Query: 92  KPYGKNCKSSEANPYDRGCAGIYNRC 112
           + Y   C    +NPY RGC+  Y RC
Sbjct: 89  ESYSTQCLPPPSNPYSRGCSKHY-RC 113

[Close]

BLAST of Cla021855 vs. Swiss-Prot
Match: RLF24_ARATH (Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-07
Identity = 32/74 (43.24%), Postives = 42/74 (56.76%), Query Frame = 1


Query: 38  NGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCNKGAGKPYGKNCKSSEA 97
           NG I E  EE E +M SEISRR +  + ++ISY+ L +D   C K     Y   C+S +A
Sbjct: 41  NGVIGE--EEGEEMMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYA--CRSGQA 100

Query: 98  NPYDRGCAGIYNRC 112
           N Y+RGC+ +  RC
Sbjct: 101 NAYNRGCS-VITRC 109

[Close]

BLAST of Cla021855 vs. TrEMBL
Match: A0A0A0L9A8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.0e-18
Identity = 55/111 (49.55%), Postives = 74/111 (66.67%), Query Frame = 1


Query: 6   SHQSLGVVLGLLYLLIFSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKT 65
           S Q+  ++L  L+L +F ++ ++   S T  CNGSIA C  EEEMLMESEI+RRFLEQ+ 
Sbjct: 18  SLQTCFLLLLFLFLFLFQDLCLVRASS-THSCNGSIAACANEEEMLMESEITRRFLEQQK 77

Query: 66  KHISYDVLIKDKPTCN-KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
           K+IS   L KD P C+    G+PY +  +C    ANPY+RGC+ IY RCR+
Sbjct: 78  KYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIY-RCRS 126

[Close]

BLAST of Cla021855 vs. TrEMBL
Match: C6T1A5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.0e-17
Identity = 52/101 (51.49%), Postives = 67/101 (66.34%), Query Frame = 1


Query: 16  LLYLLIFSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIK 75
           L   + FS+  V ++ S  S CNGSIAEC +E+E+LMESEISRRFLEQK  +IS   L +
Sbjct: 18  LFSFMHFSSCTVFSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQR 77

Query: 76  DKPTCN-KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
           DKP CN  G+G+ Y K   C    +NP +RGC+  Y RCR+
Sbjct: 78  DKPVCNGGGSGEAYSKTGGCLPPPSNPQNRGCSKYY-RCRS 117

[Close]

BLAST of Cla021855 vs. TrEMBL
Match: C6TLU4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.4e-17
Identity = 50/95 (52.63%), Postives = 65/95 (68.42%), Query Frame = 1


Query: 22  FSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCN 81
           F++  V+++ S  S CNGSIAEC +E+E+LMESEISRRFLEQK  +IS   L +DKP CN
Sbjct: 24  FTSCTVLSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQRDKPVCN 83

Query: 82  -KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
             G+G+ Y K   C    +NP  RGC+  Y RCR+
Sbjct: 84  GGGSGEAYSKTRGCLPPPSNPQSRGCSKYY-RCRS 117

[Close]

BLAST of Cla021855 vs. TrEMBL
Match: A0A0R0IK43_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G108200 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.4e-17
Identity = 50/95 (52.63%), Postives = 65/95 (68.42%), Query Frame = 1


Query: 22  FSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCN 81
           F++  V+++ S  S CNGSIAEC +E+E+LMESEISRRFLEQK  +IS   L +DKP CN
Sbjct: 24  FTSCTVLSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQRDKPVCN 83

Query: 82  -KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
             G+G+ Y K   C    +NP  RGC+  Y RCR+
Sbjct: 84  GGGSGEAYSKTGGCLPPPSNPQSRGCSKYY-RCRS 117

[Close]

BLAST of Cla021855 vs. TrEMBL
Match: K7L602_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.4e-17
Identity = 50/95 (52.63%), Postives = 65/95 (68.42%), Query Frame = 1


Query: 22  FSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIKDKPTCN 81
           F++  V+++ S  S CNGSIAEC +E+E+LMESEISRRFLEQK  +IS   L +DKP CN
Sbjct: 86  FTSCTVLSLKSHASTCNGSIAECNQEDELLMESEISRRFLEQKRSYISNGALQRDKPVCN 145

Query: 82  -KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
             G+G+ Y K   C    +NP  RGC+  Y RCR+
Sbjct: 146 GGGSGEAYSKTGGCLPPPSNPQSRGCSKYY-RCRS 179

[Close]

BLAST of Cla021855 vs. NCBI nr
Match: gi|698576934|ref|XP_009776339.1| (PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 99.8 bits (247), Expect = 3.3e-18
Identity = 51/100 (51.00%), Postives = 70/100 (70.00%), Query Frame = 1


Query: 16  LLYLLIFSNIVVIAIDSLT--SPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVL 75
           L+Y+      ++  I S+T  SPCNG+IAEC EE E+LMES+ISRRFLE++ K+IS   L
Sbjct: 9   LIYIFSIFLTLLFIITSVTGQSPCNGTIAECNEELEILMESDISRRFLEERKKYISPGAL 68

Query: 76  IKDKPTCN-KGAGKPYGKNCKSSEANPYDRGCAGIYNRCR 113
            +D+P CN  G G+PY ++C    +NPY+RGC+  Y RCR
Sbjct: 69  KRDQPVCNGGGGGEPYSRSCLPPPSNPYNRGCSKYY-RCR 107

[Close]

BLAST of Cla021855 vs. NCBI nr
Match: gi|697103835|ref|XP_009605720.1| (PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis])

HSP 1 Score: 99.4 bits (246), Expect = 4.4e-18
Identity = 51/100 (51.00%), Postives = 69/100 (69.00%), Query Frame = 1


Query: 16  LLYLLIFSNIVVIAIDSLT--SPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVL 75
           +LY       ++  I S+T  SPCNG+IAEC EE E+LMES+ISRRFLE++ K+IS   L
Sbjct: 9   ILYFFTLFLTLLFIITSVTGQSPCNGTIAECNEELEILMESDISRRFLEERKKYISPGAL 68

Query: 76  IKDKPTCN-KGAGKPYGKNCKSSEANPYDRGCAGIYNRCR 113
            +D+P CN  G G+PY ++C    +NPY+RGC+  Y RCR
Sbjct: 69  KRDQPVCNGGGGGEPYSRSCLPPPSNPYNRGCSKYY-RCR 107

[Close]

BLAST of Cla021855 vs. NCBI nr
Match: gi|778679218|ref|XP_004147646.2| (PREDICTED: protein RALF-like 32 [Cucumis sativus])

HSP 1 Score: 99.0 bits (245), Expect = 5.7e-18
Identity = 55/111 (49.55%), Postives = 74/111 (66.67%), Query Frame = 1


Query: 6   SHQSLGVVLGLLYLLIFSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKT 65
           S Q+  ++L  L+L +F ++ ++   S T  CNGSIA C  EEEMLMESEI+RRFLEQ+ 
Sbjct: 18  SLQTCFLLLLFLFLFLFQDLCLVRASS-THSCNGSIAACANEEEMLMESEITRRFLEQQK 77

Query: 66  KHISYDVLIKDKPTCN-KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
           K+IS   L KD P C+    G+PY +  +C    ANPY+RGC+ IY RCR+
Sbjct: 78  KYISIGALKKDHPACDGASGGQPYTRSGSCAPPPANPYNRGCSKIY-RCRS 126

[Close]

BLAST of Cla021855 vs. NCBI nr
Match: gi|697128698|ref|XP_009618400.1| (PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis])

HSP 1 Score: 98.6 bits (244), Expect = 7.4e-18
Identity = 52/98 (53.06%), Postives = 69/98 (70.41%), Query Frame = 1


Query: 18  YLLIFSNIVVIAIDSLT--SPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDVLIK 77
           +  IF  ++ I I S+T  SPCNG+IAEC EE E+LMES+ISRRFLE++ K+IS   L +
Sbjct: 34  FFTIFLTLLFI-ITSVTGQSPCNGTIAECNEELEILMESDISRRFLEERKKYISPGALKR 93

Query: 78  DKPTCNK-GAGKPYGKNCKSSEANPYDRGCAGIYNRCR 113
           D+P CN  G G+PY + C    +NPY+RGC+  Y RCR
Sbjct: 94  DQPVCNSGGGGEPYSRRCLPPPSNPYNRGCSKYY-RCR 129

[Close]

BLAST of Cla021855 vs. NCBI nr
Match: gi|659077106|ref|XP_008439036.1| (PREDICTED: protein RALF-like 32 [Cucumis melo])

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-17
Identity = 54/104 (51.92%), Postives = 69/104 (66.35%), Query Frame = 1


Query: 13  VLGLLYLLIFSNIVVIAIDSLTSPCNGSIAECYEEEEMLMESEISRRFLEQKTKHISYDV 72
           +L LL+L +  ++ ++   S T  CNGSIAEC  E+E+LMESEISRRFL Q+ K+IS   
Sbjct: 17  LLLLLFLFLLQDLCLVRASS-THSCNGSIAECGSEDEILMESEISRRFLAQQKKYISIGA 76

Query: 73  LIKDKPTCN-KGAGKPYGK--NCKSSEANPYDRGCAGIYNRCRT 114
           L KD P C+    G+PY K  +C    ANPYDRGC  IY RCR+
Sbjct: 77  LKKDHPACDGASGGRPYTKSGSCAPPPANPYDRGCPKIY-RCRS 118

The following BLAST results are available for this feature:

BLAST of Cla021855 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF22_ARATH	3.0e-11	50.60	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]
RLF1_ARATH	1.3e-09	47.50	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RLF32_ARATH	1.3e-09	40.70	Protein RALF-like 32 OS=Arabidopsis thaliana GN=RALFL32 PE=3 SV=1	[more]
RLF24_ARATH	1.2e-07	43.24	Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1	[more]

BLAST of Cla021855 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9A8_CUCSA	4.0e-18	49.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171840 PE=4 SV=1	[more]
C6T1A5_SOYBN	2.0e-17	51.49	Uncharacterized protein OS=Glycine max GN=GLYMA_05G151400 PE=2 SV=1	[more]
C6TLU4_SOYBN	4.4e-17	52.63	Putative uncharacterized protein OS=Glycine max PE=2 SV=1	[more]
A0A0R0IK43_SOYBN	4.4e-17	52.63	Uncharacterized protein OS=Glycine max GN=GLYMA_08G108200 PE=4 SV=1	[more]
K7L602_SOYBN	4.4e-17	52.63	Uncharacterized protein OS=Glycine max PE=4 SV=1	[more]

BLAST of Cla021855 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|698576934\|ref\|XP_009776339.1\|	3.3e-18	51.00	PREDICTED: protein RALF-like 32 isoform X1 [Nicotiana sylvestris]	[more]
gi\|697103835\|ref\|XP_009605720.1\|	4.4e-18	51.00	PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis]	[more]
gi\|778679218\|ref\|XP_004147646.2\|	5.7e-18	49.55	PREDICTED: protein RALF-like 32 [Cucumis sativus]	[more]
gi\|697128698\|ref\|XP_009618400.1\|	7.4e-18	53.06	PREDICTED: protein RALF-like 32 [Nicotiana tomentosiformis]	[more]
gi\|659077106\|ref\|XP_008439036.1\|	1.7e-17	51.92	PREDICTED: protein RALF-like 32 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla021855	Cla021855.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 48..112 score: 7.2
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 12..112 score: 6.6
None	No IPR available	PANTHER	PTHR33136:SF3	PROTEIN RALF-LIKE 32	coord: 12..112 score: 6.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla021855	CmaCh14G015310	Cucurbita maxima (Rimu)	cmawmB242
Cla021855	Cp4.1LG03g12260	Cucurbita pepo (Zucchini)	cpewmB613
Cla021855	Carg24782	Silver-seed gourd	carwmB1043

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla021855	Cucurbita pepo (Zucchini)	cpewmB845
Cla021855	Bottle gourd (USVL1VR-Ls)	lsiwmB366
Cla021855	Cucumber (Gy14) v2	cgybwmB217
Cla021855	Melon (DHL92) v3.6.1	medwmB447
Cla021855	Silver-seed gourd	carwmB0692
Cla021855	Cucumber (Chinese Long) v3	cucwmB239
Cla021855	Watermelon (97103) v2	wmwmbB206
Cla021855	Wax gourd	wgowmB201
Cla021855	Cucumber (Gy14) v1	cgywmB479
Cla021855	Cucurbita maxima (Rimu)	cmawmB798
Cla021855	Cucurbita moschata (Rifu)	cmowmB231
Cla021855	Cucurbita moschata (Rifu)	cmowmB793
Cla021855	Melon (DHL92) v3.5.1	mewmB461
Cla021855	Watermelon (Charleston Gray)	wcgwmB286
Cla021855	Cucumber (Chinese Long) v2	cuwmB233