Lsi05G022300 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G022300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ethylene-responsive transcription factor 13
Location	chr05 : 28858778 .. 28860206 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGCGTCCTTTTGAATGGGACTTATCAGTCAATAGATAATTACAAATCACCTCGCTCTGAAGACAGATATCTGCTCTGACTGTCAACGTAAGACCCATAAATTAGTTCTGAGGTTTCTGAGGAGACTGACAAACCGCCGGATATAGCTTTCTGTAATTGTTTATCAATGGTCACCTTGATCTTATATATTTCAATTCTATTTGGGGTATTTGACAACTCAGCATTTTCTTTTTCCTCCAGCCTTTTGACGTGATCGTTGAAGTTAATAGAATGGACAAGAGGTTTTAATCAAACGCCAAAATTACTATTGTATTTGCGGAAAACTTTGATGACCTTTTCTATTGCAAATGGCTCCAATTTAGCGGTAAGTTTTCAACATTTATATATTATATAAGAAGTAACCAAGAACATTCAAGTGAGAACTGTCCTTTATTAACAAATACTTGAAGCGCACGTTTTCAATGTTATCTAGCAAGAGTGCTGTGTGATGCTCCTTTTTAAATATTGCTACTCCAATTTATTTAAGAATTATTTTGGAATTTAAAATAAGGACAATATCATATGACATAAATTCGTAGTGGCAGGCAGATTATAAGAAAATGAAAGAAGTACAGTCAAACTATTCAATTTTTTATTCTAAATAGACATTTTTACATATGTGTATATATATATTTCATATATCACTGGTACCTAATATTTATGTGAACACGTGGTGTGGTTTGGAAGATTCAAAGAAAGTGGTGGTTGCGTTGTATTACTATTATATATAAAAGGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

mRNA sequence

ATGTCTGCGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

Coding sequence (CDS)

ATGTCTGCGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

Protein sequence

MSAGRDDGDTECGSSKLRQKRIRSRKMWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPPPLFNFNNY

Homology

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BLAST of Lsi05G022300 vs. Swiss-Prot
Match: ERF99_ARATH (Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana GN=ERF13 PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.8e-26
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 1


Query: 118 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 177
           R +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY T +DAA+AYDRAAFQ+RG+KAK
Sbjct: 86  RQKGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYETPEDAAVAYDRAAFQLRGSKAK 145

Query: 178 LNFPHMIASTSNNDYPRRSCKRS 201
           LNFPH+I S        R  +RS
Sbjct: 146 LNFPHLIGSCKYEPVRIRPRRRS 168

[Close]

BLAST of Lsi05G022300 vs. Swiss-Prot
Match: ERF2_TOBAC (Ethylene-responsive transcription factor 2 OS=Nicotiana tabacum GN=ERF2 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.7e-23
Identity = 51/83 (61.45%), Postives = 67/83 (80.72%), Query Frame = 1


Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           +G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGTY TA++AALAYD+AA++MRG+KA LN
Sbjct: 95  KGRHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTYETAEEAALAYDKAAYRMRGSKALLN 154

Query: 180 FPHMIASTSNNDYPRRSCKRSSP 203
           FPH I   +  +  R + KR SP
Sbjct: 155 FPHRI-GLNEPEPVRLTAKRRSP 176

[Close]

BLAST of Lsi05G022300 vs. Swiss-Prot
Match: ERF1_TOBAC (Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum GN=ERF1 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 49/83 (59.04%), Postives = 64/83 (77.11%), Query Frame = 1


Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           RG  +RGVRRRPWGK+AAEIRDP++ GAR+WLGTY T ++AA+AYD+AA++MRG+KA LN
Sbjct: 101 RGRHYRGVRRRPWGKFAAEIRDPAKNGARVWLGTYETDEEAAIAYDKAAYRMRGSKAHLN 160

Query: 180 FPHMIASTSNNDYPRRSCKRSSP 203
           FPH I           + +R+SP
Sbjct: 161 FPHRIGLNEPEPVRVTAKRRASP 183

[Close]

BLAST of Lsi05G022300 vs. Swiss-Prot
Match: PTI5_SOLLC (Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicum GN=PTI5 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 50/68 (73.53%), Postives = 59/68 (86.76%), Query Frame = 1


Query: 121 GLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNF 180
           G ++RGVRRRPWGKYAAEIRD +R GAR+WLGT+ TA++AALAYDRAAF+MRGAKA LNF
Sbjct: 56  GKKYRGVRRRPWGKYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNF 115

Query: 181 PHMIASTS 189
           P  I + S
Sbjct: 116 PSEIVNAS 123

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BLAST of Lsi05G022300 vs. Swiss-Prot
Match: ERF2_NICSY (Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris GN=ERF2 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 53/92 (57.61%), Postives = 69/92 (75.00%), Query Frame = 1


Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           +G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGTY TA++AALAYD+AA++MRG+KA LN
Sbjct: 99  KGRHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTYETAEEAALAYDKAAYRMRGSKALLN 158

Query: 180 FPHMIASTSNNDYPRRSCKRSSPNCNSPRTPP 212
           FPH I   +  +  R + KR SP   S    P
Sbjct: 159 FPHRI-GLNEPEPVRLTVKRRSPEPASSSISP 189

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BLAST of Lsi05G022300 vs. TrEMBL
Match: A0A0A0L893_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116730 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.1e-43
Identity = 107/165 (64.85%), Postives = 128/165 (77.58%), Query Frame = 1


Query: 30  NSNSSSLEGSS-DLFVLERIRRHLLEDELG-----NNVDEEGRWEWGNNSTGGGSKSNDN 89
           +S+SSSLEGSS DLF LE+IRRHLLED+LG     NNV E+G   +  + +G G + ++N
Sbjct: 3   SSSSSSLEGSSSDLFHLEQIRRHLLEDDLGKGSTHNNV-EDGIIRYDISRSGAGWEMSNN 62

Query: 90  NNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPS 149
           NNNMV     ++M       S II  + +++ Q R RGLQFRGVRRRPWGKYAAEIRDPS
Sbjct: 63  NNNMV-----DQM------SSIIIRDNPEEKSQPRPRGLQFRGVRRRPWGKYAAEIRDPS 122

Query: 150 RKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           +KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 123 KKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 155

[Close]

BLAST of Lsi05G022300 vs. TrEMBL
Match: A0A0D2RDC4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G108500 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.1e-27
Identity = 70/123 (56.91%), Postives = 85/123 (69.11%), Query Frame = 1


Query: 88  VMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGA 147
           V+V  GEE  G       I+    +    +R RG+ F+GVRRRPWGKYAAEIRDP R G+
Sbjct: 103 VVVSGGEEKEG-------IVNVERETNAPLRPRGMNFKGVRRRPWGKYAAEIRDPKRNGS 162

Query: 148 RIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSP 207
           RIWLGTY T +DAALAYDRAAF+MRGAKAKLNFPH+I S + +D  R   +R SP  ++ 
Sbjct: 163 RIWLGTYETPEDAALAYDRAAFEMRGAKAKLNFPHLIGS-NTSDPIRVGSRRRSPEPSAS 217

Query: 208 RTP 211
            TP
Sbjct: 223 TTP 217

[Close]

BLAST of Lsi05G022300 vs. TrEMBL
Match: B9MV98_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s04600g PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.6e-26
Identity = 62/91 (68.13%), Postives = 76/91 (83.52%), Query Frame = 1


Query: 117 IRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKA 176
           ++ +GLQ++GVRRRPWGKYAAEIRDP + GAR+WLGTY T +DAALAYDRAAF+MRG+KA
Sbjct: 111 LKEKGLQYKGVRRRPWGKYAAEIRDPKKNGARMWLGTYETPEDAALAYDRAAFKMRGSKA 170

Query: 177 KLNFPHMIASTSNNDYPR-RSCKRSSPNCNS 207
           KLNFPH+I S +N +  R  S KRSSP  +S
Sbjct: 171 KLNFPHLIGSNNNWEPTRVTSKKRSSPEPSS 201

[Close]

BLAST of Lsi05G022300 vs. TrEMBL
Match: A0A061E2E4_THECC (Ethylene-responsive element binding factor 13, putative OS=Theobroma cacao GN=TCM_005704 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.7e-26
Identity = 61/90 (67.78%), Postives = 75/90 (83.33%), Query Frame = 1


Query: 118 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 177
           R +G+ +RGVRRRPWGKYAAEIRDP +KGAR+WLGTY T +DAALAYD+AAF++RG+KAK
Sbjct: 100 RGKGVNYRGVRRRPWGKYAAEIRDPKKKGARLWLGTYETPEDAALAYDKAAFEIRGSKAK 159

Query: 178 LNFPHMIASTSNNDYPRRSC-KRSSPNCNS 207
           LNFPH+I S SN+  P R C +R SP+  S
Sbjct: 160 LNFPHLIGS-SNSTEPVRVCPRRRSPDTTS 188

[Close]

BLAST of Lsi05G022300 vs. TrEMBL
Match: A0A0A0L2V9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116720 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.5e-25
Identity = 66/117 (56.41%), Postives = 78/117 (66.67%), Query Frame = 1


Query: 94  EEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGT 153
           EE   KSG G   I      +      G+QF+GVRRRPWGKYAAEIRDP R GAR WLGT
Sbjct: 19  EENLNKSGDGICSIIQDHHYKGAAVENGVQFKGVRRRPWGKYAAEIRDPKRNGARTWLGT 78

Query: 154 YHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTP 211
           + TA +AALAYDRAAF++RGAKAKLNFPH+I S S +     S   S+ + N P +P
Sbjct: 79  FETALEAALAYDRAAFKIRGAKAKLNFPHLIDSDSTH-----STSASTSSANPPPSP 130

[Close]

BLAST of Lsi05G022300 vs. TAIR10
Match: AT2G44840.1 (AT2G44840.1 ethylene-responsive element binding factor 13)

HSP 1 Score: 119.8 bits (299), Expect = 2.1e-27
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 1


Query: 118 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 177
           R +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY T +DAA+AYDRAAFQ+RG+KAK
Sbjct: 86  RQKGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYETPEDAAVAYDRAAFQLRGSKAK 145

Query: 178 LNFPHMIASTSNNDYPRRSCKRS 201
           LNFPH+I S        R  +RS
Sbjct: 146 LNFPHLIGSCKYEPVRIRPRRRS 168

[Close]

BLAST of Lsi05G022300 vs. TAIR10
Match: AT4G17500.1 (AT4G17500.1 ethylene responsive element binding factor 1)

HSP 1 Score: 105.9 bits (263), Expect = 3.2e-23
Identity = 53/93 (56.99%), Postives = 71/93 (76.34%), Query Frame = 1


Query: 119 ARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKL 178
           A+G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGT+ TA+DAALAYDRAAF+MRG++A L
Sbjct: 143 AKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALL 202

Query: 179 NFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPP 212
           NFP +  ++   D  R   KRSS + ++    P
Sbjct: 203 NFP-LRVNSGEPDPVRIKSKRSSFSSSNENGAP 234

[Close]

BLAST of Lsi05G022300 vs. TAIR10
Match: AT2G33710.2 (AT2G33710.2 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 2.1e-22
Identity = 57/117 (48.72%), Postives = 73/117 (62.39%), Query Frame = 1


Query: 103 GSSIIWSSDDQ------RDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHT 162
           G  I  S DDQ      +++  +R   +RGVR+RPWGK+AAEIRDP+ K AR+WLGT+ T
Sbjct: 43  GQPIPSSIDDQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPN-KAARVWLGTFDT 102

Query: 163 AQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPPPL 214
           A++AALAYD+AAF+ RG KAKLNFP  I       YP  +          P  PPP+
Sbjct: 103 AEEAALAYDKAAFEFRGHKAKLNFPEHIRVNPTQLYPSPATSHDRIIVTPPSPPPPI 158

[Close]

BLAST of Lsi05G022300 vs. TAIR10
Match: AT3G23220.1 (AT3G23220.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 102.1 bits (253), Expect = 4.6e-22
Identity = 45/61 (73.77%), Postives = 55/61 (90.16%), Query Frame = 1


Query: 122 LQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFP 181
           +++RGVR+RPWGKYAAEIRD +R GAR+WLGT++TA+DAA AYDRAAF MRG +A LNFP
Sbjct: 12  MKYRGVRKRPWGKYAAEIRDSARHGARVWLGTFNTAEDAARAYDRAAFGMRGQRAILNFP 71

Query: 182 H 183
           H
Sbjct: 72  H 72

[Close]

BLAST of Lsi05G022300 vs. TAIR10
Match: AT5G47220.1 (AT5G47220.1 ethylene responsive element binding factor 2)

HSP 1 Score: 101.7 bits (252), Expect = 6.0e-22
Identity = 50/83 (60.24%), Postives = 67/83 (80.72%), Query Frame = 1


Query: 124 FRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHM 183
           +RGVR+RPWGK+AAEIRDP++ GAR+WLGT+ TA+DAALAYD AAF+MRG++A LNFP +
Sbjct: 117 YRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDIAAFRMRGSRALLNFP-L 176

Query: 184 IASTSNNDYPRRSCKRSSPNCNS 207
             ++   D  R + KRSS + +S
Sbjct: 177 RVNSGEPDPVRITSKRSSSSSSS 198

[Close]

BLAST of Lsi05G022300 vs. NCBI nr
Match: gi|659077725|ref|XP_008439350.1| (PREDICTED: ethylene-responsive transcription factor 13-like [Cucumis melo])

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-43
Identity = 111/166 (66.87%), Postives = 129/166 (77.71%), Query Frame = 1


Query: 29  SNSNSSSLEGSS-DLFVLERIRRHLLEDELG----NNVDEEGRWEWGNNSTGGG-SKSND 88
           S+S+SSSLEGSS DLF+LE+IRRHLLED+LG    NN  E+G   +  + +G G   SN+
Sbjct: 4   SSSSSSSLEGSSSDLFLLEQIRRHLLEDDLGKGSTNNNVEDGIIRYDISCSGAGWEMSNN 63

Query: 89  NNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDP 148
           NNNNMV     ++M       SSII   D+  ++ R RGLQFRGVRRRPWGKYAAEIRDP
Sbjct: 64  NNNNMV-----DQM-------SSII-IRDNPEEKSRPRGLQFRGVRRRPWGKYAAEIRDP 123

Query: 149 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 124 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 156

[Close]

BLAST of Lsi05G022300 vs. NCBI nr
Match: gi|449432982|ref|XP_004134277.1| (PREDICTED: ethylene-responsive transcription factor 1 [Cucumis sativus])

HSP 1 Score: 183.3 bits (464), Expect = 4.4e-43
Identity = 107/165 (64.85%), Postives = 128/165 (77.58%), Query Frame = 1


Query: 30  NSNSSSLEGSS-DLFVLERIRRHLLEDELG-----NNVDEEGRWEWGNNSTGGGSKSNDN 89
           +S+SSSLEGSS DLF LE+IRRHLLED+LG     NNV E+G   +  + +G G + ++N
Sbjct: 3   SSSSSSLEGSSSDLFHLEQIRRHLLEDDLGKGSTHNNV-EDGIIRYDISRSGAGWEMSNN 62

Query: 90  NNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPS 149
           NNNMV     ++M       S II  + +++ Q R RGLQFRGVRRRPWGKYAAEIRDPS
Sbjct: 63  NNNMV-----DQM------SSIIIRDNPEEKSQPRPRGLQFRGVRRRPWGKYAAEIRDPS 122

Query: 150 RKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           +KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 123 KKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 155

[Close]

BLAST of Lsi05G022300 vs. NCBI nr
Match: gi|823201863|ref|XP_012435678.1| (PREDICTED: ethylene-responsive transcription factor 13-like [Gossypium raimondii])

HSP 1 Score: 130.2 bits (326), Expect = 4.5e-27
Identity = 70/123 (56.91%), Postives = 85/123 (69.11%), Query Frame = 1


Query: 88  VMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGA 147
           V+V  GEE  G       I+    +    +R RG+ F+GVRRRPWGKYAAEIRDP R G+
Sbjct: 140 VVVSGGEEKEG-------IVNVERETNAPLRPRGMNFKGVRRRPWGKYAAEIRDPKRNGS 199

Query: 148 RIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSP 207
           RIWLGTY T +DAALAYDRAAF+MRGAKAKLNFPH+I S + +D  R   +R SP  ++ 
Sbjct: 200 RIWLGTYETPEDAALAYDRAAFEMRGAKAKLNFPHLIGS-NTSDPIRVGSRRRSPEPSAS 254

Query: 208 RTP 211
            TP
Sbjct: 260 TTP 254

[Close]

BLAST of Lsi05G022300 vs. NCBI nr
Match: gi|763782157|gb|KJB49228.1| (hypothetical protein B456_008G108500 [Gossypium raimondii])

HSP 1 Score: 130.2 bits (326), Expect = 4.5e-27
Identity = 70/123 (56.91%), Postives = 85/123 (69.11%), Query Frame = 1


Query: 88  VMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGA 147
           V+V  GEE  G       I+    +    +R RG+ F+GVRRRPWGKYAAEIRDP R G+
Sbjct: 103 VVVSGGEEKEG-------IVNVERETNAPLRPRGMNFKGVRRRPWGKYAAEIRDPKRNGS 162

Query: 148 RIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSP 207
           RIWLGTY T +DAALAYDRAAF+MRGAKAKLNFPH+I S + +D  R   +R SP  ++ 
Sbjct: 163 RIWLGTYETPEDAALAYDRAAFEMRGAKAKLNFPHLIGS-NTSDPIRVGSRRRSPEPSAS 217

Query: 208 RTP 211
            TP
Sbjct: 223 TTP 217

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BLAST of Lsi05G022300 vs. NCBI nr
Match: gi|566202511|ref|XP_006375126.1| (hypothetical protein POPTR_0014s04600g [Populus trichocarpa])

HSP 1 Score: 127.1 bits (318), Expect = 3.8e-26
Identity = 62/91 (68.13%), Postives = 76/91 (83.52%), Query Frame = 1


Query: 117 IRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKA 176
           ++ +GLQ++GVRRRPWGKYAAEIRDP + GAR+WLGTY T +DAALAYDRAAF+MRG+KA
Sbjct: 111 LKEKGLQYKGVRRRPWGKYAAEIRDPKKNGARMWLGTYETPEDAALAYDRAAFKMRGSKA 170

Query: 177 KLNFPHMIASTSNNDYPR-RSCKRSSPNCNS 207
           KLNFPH+I S +N +  R  S KRSSP  +S
Sbjct: 171 KLNFPHLIGSNNNWEPTRVTSKKRSSPEPSS 201

The following BLAST results are available for this feature:

BLAST of Lsi05G022300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ERF99_ARATH	3.8e-26	66.27	Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana GN=ERF13 PE=...	[more]
ERF2_TOBAC	6.7e-23	61.45	Ethylene-responsive transcription factor 2 OS=Nicotiana tabacum GN=ERF2 PE=2 SV=...	[more]
ERF1_TOBAC	1.1e-22	59.04	Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum GN=ERF1 PE=2 SV=...	[more]
PTI5_SOLLC	1.1e-22	73.53	Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicu...	[more]
ERF2_NICSY	2.5e-22	57.61	Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris GN=ERF2 PE=2 ...	[more]

BLAST of Lsi05G022300 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L893_CUCSA	3.1e-43	64.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116730 PE=4 SV=1	[more]
A0A0D2RDC4_GOSRA	3.1e-27	56.91	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G108500 PE=4 SV=1	[more]
B9MV98_POPTR	2.6e-26	68.13	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s04600g PE=4 SV=1	[more]
A0A061E2E4_THECC	7.7e-26	67.78	Ethylene-responsive element binding factor 13, putative OS=Theobroma cacao GN=TC...	[more]
A0A0A0L2V9_CUCSA	8.5e-25	56.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116720 PE=4 SV=1	[more]

BLAST of Lsi05G022300 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44840.1	2.1e-27	66.27	ethylene-responsive element binding factor 13	[more]
AT4G17500.1	3.2e-23	56.99	ethylene responsive element binding factor 1	[more]
AT2G33710.2	2.1e-22	48.72	Integrase-type DNA-binding superfamily protein	[more]
AT3G23220.1	4.6e-22	73.77	Integrase-type DNA-binding superfamily protein	[more]
AT5G47220.1	6.0e-22	60.24	ethylene responsive element binding factor 2	[more]

BLAST of Lsi05G022300 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077725\|ref\|XP_008439350.1\|	1.5e-43	66.87	PREDICTED: ethylene-responsive transcription factor 13-like [Cucumis melo]	[more]
gi\|449432982\|ref\|XP_004134277.1\|	4.4e-43	64.85	PREDICTED: ethylene-responsive transcription factor 1 [Cucumis sativus]	[more]
gi\|823201863\|ref\|XP_012435678.1\|	4.5e-27	56.91	PREDICTED: ethylene-responsive transcription factor 13-like [Gossypium raimondii...	[more]
gi\|763782157\|gb\|KJB49228.1\|	4.5e-27	56.91	hypothetical protein B456_008G108500 [Gossypium raimondii]	[more]
gi\|566202511\|ref\|XP_006375126.1\|	3.8e-26	68.13	hypothetical protein POPTR_0014s04600g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR016177	DNA-bd_dom_sf
IPR001471	AP2/ERF_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0071229	cellular response to acid chemical
biological_process	GO:1901701	cellular response to oxygen-containing compound
biological_process	GO:0006952	defense response
biological_process	GO:0009755	hormone-mediated signaling pathway
biological_process	GO:0002376	immune system process
biological_process	GO:0043067	regulation of programmed cell death
biological_process	GO:0009628	response to abiotic stimulus
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009753	response to jasmonic acid
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G022300.1	Lsi05G022300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 163..183 score: 6.4E-13coord: 124..135 score: 6.4
IPR001471	AP2/ERF domain	GENE3D	G3DSA:3.30.730.10		coord: 123..182 score: 1.5
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 124..173 score: 1.8
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 123..187 score: 1.0
IPR001471	AP2/ERF domain	PROFILE	PS51032	AP2_ERF	coord: 123..181 score: 23
IPR016177	DNA-binding domain	unknown	SSF54171	DNA-binding domain	coord: 124..182 score: 1.96
None	No IPR available	PANTHER	PTHR31190	FAMILY NOT NAMED	coord: 25..210 score: 8.4
None	No IPR available	PANTHER	PTHR31190:SF21	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 13	coord: 25..210 score: 8.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G022300	Csa3G116720	Cucumber (Chinese Long) v2	culsiB209
Lsi05G022300	CSPI03G07060	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G022300	Cucsa.260030	Cucumber (Gy14) v1	cgylsiB415
Lsi05G022300	CmaCh06G009360	Cucurbita maxima (Rimu)	cmalsiB766
Lsi05G022300	CmoCh06G009640	Cucurbita moschata (Rifu)	cmolsiB758
Lsi05G022300	Cla021069	Watermelon (97103) v1	lsiwmB365
Lsi05G022300	MELO3C005940	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G022300	ClCG05G000100	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G022300	MELO3C005940.2	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G022300	Cla97C05G080020	Watermelon (97103) v2	lsiwmbB333
Lsi05G022300	Bhi01G000454	Wax gourd	lsiwgoB433
Lsi05G022300	CsGy3G007210	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G022300	Carg25064	Silver-seed gourd	carlsiB926
Lsi05G022300	CsaV3_3G007180	Cucumber (Chinese Long) v3	cuclsiB228

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G022300	Cucurbita pepo (Zucchini)	cpelsiB714