CmaCh17G003240 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh17G003240
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Rapid alkalinization factor
Location	Cma_Chr17 : 1749063 .. 1749446 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTCATCGGCAACGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCACCGAGTGCTTTCTCGCTGGAAACTATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGTCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

mRNA sequence

ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTCATCGGCAACGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCACCGAGTGCTTTCTCGCTGGAAACTATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGTCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

Coding sequence (CDS)

ATGGGTATCTCACGCGTTTCTGCTCTCATTGCTGCGTTCGCCGTTCTCGCCGCTCATTTTCTGATCTCATCGGCAACGGCCATCGATTTCTCCGGCGACAACGAGATTCTCTTTGCTCCGACTAAATCCGAGTGCCGAGGCTCAATCACCGAGTGCTTTCTCGCTGGAAACTATGACGATTCCGGCTTCGGAATGGAGTTCGAGATGGACTCTGAGATCAATCGCCGAATTCTAGCGACTACTCGCTACATCAGCTATGGTGCTCTGAGGAGGAACAACGTTCCTTGCTCCCGCCGCGGCGCTTCTTACTACAACTGTCGTCCAGGCGCTCAGGCGAATCCTTACACCCGTGGTTGTAGCGCCATTACTCGCTGCCGAAGTTAA

Protein sequence

MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCRS

Homology

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BLAST of CmaCh17G003240 vs. Swiss-Prot
Match: RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 3.7e-34
Identity = 82/138 (59.42%), Postives = 96/138 (69.57%), Query Frame = 1


Query: 2   GISRVSALIAAFAVL---AAH--FLISSATAIDFSGDNEILFAPTKSECRGSITECFLAG 61
           G+SR S   A FA+L   A H   +  S+ + +F+GD    F P ++ECRG+I EC ++ 
Sbjct: 3   GLSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSA 62

Query: 62  NYDDSG--------FGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRP 121
              D G         G EFEMDSEINRRILAT RYISYGALRRN +PCSRRGASYYNCR 
Sbjct: 63  ALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRR 122

Query: 122 GAQANPYTRGCSAITRCR 127
           GAQANPY+RGCSAITRCR
Sbjct: 123 GAQANPYSRGCSAITRCR 136

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BLAST of CmaCh17G003240 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.4e-33
Identity = 75/113 (66.37%), Postives = 86/113 (76.11%), Query Frame = 1


Query: 14  AVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDDSGFGMEFEMDSEI 73
           A+L  HFL ++ T+   SGD    F P +S+C G+I EC L+   +      EFEMDSEI
Sbjct: 14  AILTVHFLFAAVTS-QSSGD----FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 127
           NRRILATT+YISYGALRRN VPCSRRGASYYNCR GAQANPY+RGCSAITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

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BLAST of CmaCh17G003240 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.6e-29
Identity = 68/113 (60.18%), Postives = 80/113 (70.80%), Query Frame = 1


Query: 15  VLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDDSGFGMEFEMDSEIN 74
           VL   F IS A A D    + ++ A +   C+GSI EC             EFE+DSE N
Sbjct: 11  VLIGAFFISMAAAGDSGAYDWVMPARSGGGCKGSIGECIAEEE--------EFELDSESN 70

Query: 75  RRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCRS 128
           RRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQANPY+RGCSAITRCRS
Sbjct: 71  RRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

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BLAST of CmaCh17G003240 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.2e-24
Identity = 66/123 (53.66%), Postives = 77/123 (62.60%), Query Frame = 1


Query: 9   LIAAFAVLAAHFLISSATAIDFSGD-NEILFAPTK---SECRGSITECFLAGNYDDSGFG 68
           L     +L    + S      F+ D   + +A T    S C GSI EC  A         
Sbjct: 7   LFLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAE-------- 66

Query: 69  MEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITR 128
            E EMDSEINRRILATT+YISY +L+RN+VPCSRRGASYYNC+ GAQANPY+RGCS I R
Sbjct: 67  -EEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIAR 120

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BLAST of CmaCh17G003240 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 7.8e-24
Identity = 64/127 (50.39%), Postives = 79/127 (62.20%), Query Frame = 1


Query: 3   ISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKS---ECRGSITECFLAGNYD 62
           ++   A+ A  A+LA       +T  DF    + + A + S    C GSI EC       
Sbjct: 1   MTNTRAIYAVIAILAIVISAVESTG-DFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-- 60

Query: 63  DSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGC 122
                 E E DS+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNC+ GAQANPY+RGC
Sbjct: 61  ------EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGC 118

Query: 123 SAITRCR 127
           S ITRCR
Sbjct: 121 STITRCR 118

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BLAST of CmaCh17G003240 vs. TrEMBL
Match: A0A0A0KBU7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G194150 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 5.6e-45
Identity = 97/127 (76.38%), Postives = 106/127 (83.46%), Query Frame = 1


Query: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60
           MGISRVS+L+A F +LA HF+ S A A+DFSGD+E+LF PT S+        F   N DD
Sbjct: 1   MGISRVSSLLAVFLILAPHFVFSMAAAVDFSGDHELLFVPTTSD--------FFDDN-DD 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
            GFGMEF+MDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS
Sbjct: 61  FGFGMEFQMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 118

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 118

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BLAST of CmaCh17G003240 vs. TrEMBL
Match: A0A0D2TAB6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G190000 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 8.1e-36
Identity = 86/128 (67.19%), Postives = 94/128 (73.44%), Query Frame = 1


Query: 1   MGISRVS-ALIAAFAVLAAHFLIS-SATAIDFSGDNEILFAPTKSECRGSITECFLAGNY 60
           MG SR S + +A  A+LA H   S S+  +DF GD+   F P KSEC GSI EC +    
Sbjct: 25  MGSSRFSFSFLAICAILAIHVAASASSPMLDFGGDH---FLPIKSECGGSIAECLMLSGE 84

Query: 61  DDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRG 120
           D S F  EF MDSEINRRILAT RYISYGALRRN VPCSRRGASYYNCRPGAQANPY+RG
Sbjct: 85  DSSEFDGEFAMDSEINRRILATRRYISYGALRRNTVPCSRRGASYYNCRPGAQANPYSRG 144

Query: 121 CSAITRCR 127
           CS ITRCR
Sbjct: 145 CSRITRCR 149

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BLAST of CmaCh17G003240 vs. TrEMBL
Match: A0A061EQ76_THECC (Rapid alkalinization factor 1, putative OS=Theobroma cacao GN=TCM_021204 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.8e-35
Identity = 85/129 (65.89%), Postives = 98/129 (75.97%), Query Frame = 1


Query: 1   MGISRVS-ALIAAFAVLAAHFLIS-SATAIDFSGDNEILFAPTKSECRGSITECF-LAGN 60
           MG SR S +L+A  A+LA H  +S S+  +DF G ++  F P KSECRGSI EC  L+G+
Sbjct: 1   MGSSRFSLSLLAICAILAVHVAVSASSPTLDFVGGDQ--FLPIKSECRGSIAECLMLSGD 60

Query: 61  YDDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTR 120
              S F  EF M SEI RRILATTRYISYGALRRN VPCSRRGASYYNC+PGAQANPY+R
Sbjct: 61  ESSSDFDAEFAMGSEIYRRILATTRYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSR 120

Query: 121 GCSAITRCR 127
           GC+ ITRCR
Sbjct: 121 GCNRITRCR 127

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BLAST of CmaCh17G003240 vs. TrEMBL
Match: G7JLH2_MEDTR (RALF OS=Medicago truncatula GN=MTR_4g119860 PE=4 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 2.6e-34
Identity = 81/117 (69.23%), Postives = 92/117 (78.63%), Query Frame = 1


Query: 13  FAVLAAHFLISSATAIDFSGDNEI--LFAPTKSECRGSITEC-FLAGNYDDSGFGMEFEM 72
           F++LA H  +SS++ +DF+ DN++   F P+ S CRGSI EC  LAG+ DDS    EF M
Sbjct: 14  FSILALHVALSSSSTLDFT-DNQLSSFFLPSDSGCRGSIAECSLLAGDEDDS----EFMM 73

Query: 73  DSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 127
           DSE NRRILA  RYISYGALRRN VPCSRRGASYYNCRPGAQANPY RGCSAITRCR
Sbjct: 74  DSESNRRILAARRYISYGALRRNTVPCSRRGASYYNCRPGAQANPYRRGCSAITRCR 125

[Close]

BLAST of CmaCh17G003240 vs. TrEMBL
Match: A5BA80_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0060g02150 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.6e-34
Identity = 78/121 (64.46%), Postives = 95/121 (78.51%), Query Frame = 1


Query: 10  IAAFAVLAAHFLI--SSATAIDFSGDNEILFAPTKSECRGSITECF-LAGNYDDSGFGME 69
           +   A+ AA FLI  S+ TA+D + DN + + PT+S C+G++ EC  LAG  D+ G   E
Sbjct: 5   VGLLAICAAAFLIAASAVTAVDSTADN-LSWIPTRSVCKGTVAECLALAGGEDEFGLTSE 64

Query: 70  FEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 128
           F MDSEINRRILAT++YISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS ITRCR
Sbjct: 65  FAMDSEINRRILATSKYISYGALQRNSVPCSRRGASYYNCQPGAQANPYNRGCSTITRCR 124

[Close]

BLAST of CmaCh17G003240 vs. TAIR10
Match: AT3G16570.1 (AT3G16570.1 rapid alkalinization factor 23)

HSP 1 Score: 145.6 bits (366), Expect = 2.1e-35
Identity = 82/138 (59.42%), Postives = 96/138 (69.57%), Query Frame = 1


Query: 2   GISRVSALIAAFAVL---AAH--FLISSATAIDFSGDNEILFAPTKSECRGSITECFLAG 61
           G+SR S   A FA+L   A H   +  S+ + +F+GD    F P ++ECRG+I EC ++ 
Sbjct: 3   GLSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSA 62

Query: 62  NYDDSG--------FGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRP 121
              D G         G EFEMDSEINRRILAT RYISYGALRRN +PCSRRGASYYNCR 
Sbjct: 63  ALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRR 122

Query: 122 GAQANPYTRGCSAITRCR 127
           GAQANPY+RGCSAITRCR
Sbjct: 123 GAQANPYSRGCSAITRCR 136

[Close]

BLAST of CmaCh17G003240 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 142.9 bits (359), Expect = 1.4e-34
Identity = 75/113 (66.37%), Postives = 86/113 (76.11%), Query Frame = 1


Query: 14  AVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDDSGFGMEFEMDSEI 73
           A+L  HFL ++ T+   SGD    F P +S+C G+I EC L+   +      EFEMDSEI
Sbjct: 14  AILTVHFLFAAVTS-QSSGD----FVPIESKCNGTIAECSLSTAEE------EFEMDSEI 73

Query: 74  NRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 127
           NRRILATT+YISYGALRRN VPCSRRGASYYNCR GAQANPY+RGCSAITRCR
Sbjct: 74  NRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of CmaCh17G003240 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 114.0 bits (284), Expect = 6.8e-26
Identity = 66/123 (53.66%), Postives = 77/123 (62.60%), Query Frame = 1


Query: 9   LIAAFAVLAAHFLISSATAIDFSGD-NEILFAPTK---SECRGSITECFLAGNYDDSGFG 68
           L     +L    + S      F+ D   + +A T    S C GSI EC  A         
Sbjct: 7   LFLTLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAE-------- 66

Query: 69  MEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITR 128
            E EMDSEINRRILATT+YISY +L+RN+VPCSRRGASYYNC+ GAQANPY+RGCS I R
Sbjct: 67  -EEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIAR 120

[Close]

BLAST of CmaCh17G003240 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 111.3 bits (277), Expect = 4.4e-25
Identity = 64/127 (50.39%), Postives = 79/127 (62.20%), Query Frame = 1


Query: 3   ISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKS---ECRGSITECFLAGNYD 62
           ++   A+ A  A+LA       +T  DF    + + A + S    C GSI EC       
Sbjct: 1   MTNTRAIYAVIAILAIVISAVESTG-DFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE-- 60

Query: 63  DSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGC 122
                 E E DS+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNC+ GAQANPY+RGC
Sbjct: 61  ------EMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGC 118

Query: 123 SAITRCR 127
           S ITRCR
Sbjct: 121 STITRCR 118

[Close]

BLAST of CmaCh17G003240 vs. TAIR10
Match: AT2G33775.1 (AT2G33775.1 ralf-like 19)

HSP 1 Score: 89.0 bits (219), Expect = 2.3e-18
Identity = 42/62 (67.74%), Postives = 49/62 (79.03%), Query Frame = 1


Query: 65  MEFEMDSEINRRILATTR-YISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAIT 124
           +++ MDSE NRR LA  R YISYGALR+NNVPCSRRG SYY+C+   +ANPY RGCS IT
Sbjct: 44  LDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVIT 103

Query: 125 RC 126
            C
Sbjct: 104 HC 105

[Close]

BLAST of CmaCh17G003240 vs. NCBI nr
Match: gi|449450680|ref|XP_004143090.1| (PREDICTED: protein RALF-like 33 [Cucumis sativus])

HSP 1 Score: 188.3 bits (477), Expect = 8.0e-45
Identity = 97/127 (76.38%), Postives = 106/127 (83.46%), Query Frame = 1


Query: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60
           MGISRVS+L+A F +LA HF+ S A A+DFSGD+E+LF PT S+        F   N DD
Sbjct: 1   MGISRVSSLLAVFLILAPHFVFSMAAAVDFSGDHELLFVPTTSD--------FFDDN-DD 60

Query: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120
            GFGMEF+MDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS
Sbjct: 61  FGFGMEFQMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 118

Query: 121 AITRCRS 128
           AITRCRS
Sbjct: 121 AITRCRS 118

[Close]

BLAST of CmaCh17G003240 vs. NCBI nr
Match: gi|659117160|ref|XP_008458453.1| (PREDICTED: protein RALF-like 33 [Cucumis melo])

HSP 1 Score: 185.7 bits (470), Expect = 5.2e-44
Identity = 97/128 (75.78%), Postives = 109/128 (85.16%), Query Frame = 1


Query: 1   MGISRVSA-LIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYD 60
           MGISRVS+ L+AAF ++AAHFL+S A A+DFSGD+++LF PT S+        F   N D
Sbjct: 1   MGISRVSSSLLAAFLIIAAHFLVSMAAAVDFSGDHKLLFVPTTSD--------FFNDN-D 60

Query: 61  DSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGC 120
           D GFGMEF+MDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPY+RGC
Sbjct: 61  DFGFGMEFQMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYSRGC 119

Query: 121 SAITRCRS 128
           SAITRCRS
Sbjct: 121 SAITRCRS 119

[Close]

BLAST of CmaCh17G003240 vs. NCBI nr
Match: gi|823244996|ref|XP_012455175.1| (PREDICTED: protein RALF-like 33 [Gossypium raimondii])

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-35
Identity = 86/128 (67.19%), Postives = 94/128 (73.44%), Query Frame = 1


Query: 1   MGISRVS-ALIAAFAVLAAHFLIS-SATAIDFSGDNEILFAPTKSECRGSITECFLAGNY 60
           MG SR S + +A  A+LA H   S S+  +DF GD+   F P KSEC GSI EC +    
Sbjct: 1   MGSSRFSFSFLAICAILAIHVAASASSPMLDFGGDH---FLPIKSECGGSIAECLMLSGE 60

Query: 61  DDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRG 120
           D S F  EF MDSEINRRILAT RYISYGALRRN VPCSRRGASYYNCRPGAQANPY+RG
Sbjct: 61  DSSEFDGEFAMDSEINRRILATRRYISYGALRRNTVPCSRRGASYYNCRPGAQANPYSRG 120

Query: 121 CSAITRCR 127
           CS ITRCR
Sbjct: 121 CSRITRCR 125

[Close]

BLAST of CmaCh17G003240 vs. NCBI nr
Match: gi|763805740|gb|KJB72678.1| (hypothetical protein B456_011G190000 [Gossypium raimondii])

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-35
Identity = 86/128 (67.19%), Postives = 94/128 (73.44%), Query Frame = 1


Query: 1   MGISRVS-ALIAAFAVLAAHFLIS-SATAIDFSGDNEILFAPTKSECRGSITECFLAGNY 60
           MG SR S + +A  A+LA H   S S+  +DF GD+   F P KSEC GSI EC +    
Sbjct: 25  MGSSRFSFSFLAICAILAIHVAASASSPMLDFGGDH---FLPIKSECGGSIAECLMLSGE 84

Query: 61  DDSGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRG 120
           D S F  EF MDSEINRRILAT RYISYGALRRN VPCSRRGASYYNCRPGAQANPY+RG
Sbjct: 85  DSSEFDGEFAMDSEINRRILATRRYISYGALRRNTVPCSRRGASYYNCRPGAQANPYSRG 144

Query: 121 CSAITRCR 127
           CS ITRCR
Sbjct: 145 CSRITRCR 149

[Close]

BLAST of CmaCh17G003240 vs. NCBI nr
Match: gi|720043259|ref|XP_010269500.1| (PREDICTED: rapid alkalinization factor-like [Nelumbo nucifera])

HSP 1 Score: 154.8 bits (390), Expect = 9.8e-35
Identity = 78/121 (64.46%), Postives = 90/121 (74.38%), Query Frame = 1


Query: 7   SALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDDSGFGME 66
           S     F V     LISS+T ID SG+ ++ + PT+S C+GS+ EC   GN        E
Sbjct: 27  SGFFVMFVVFVTALLISSSTTIDASGEYQLGWLPTRSTCQGSMEECMAGGN--------E 86

Query: 67  FEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 126
           FEMDSEINRRILAT++YISYGAL+RN VPCSRRGASYYNC+PGAQANPY RGCSAITRCR
Sbjct: 87  FEMDSEINRRILATSQYISYGALQRNTVPCSRRGASYYNCKPGAQANPYNRGCSAITRCR 139

Query: 127 S 128
           S
Sbjct: 147 S 139

The following BLAST results are available for this feature:

BLAST of CmaCh17G003240 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF23_ARATH	3.7e-34	59.42	Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1	[more]
RLF33_ARATH	2.4e-33	66.37	Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1	[more]
RALF_TOBAC	1.6e-29	60.18	Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1	[more]
RLF1_ARATH	1.2e-24	53.66	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RLF22_ARATH	7.8e-24	50.39	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]

BLAST of CmaCh17G003240 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBU7_CUCSA	5.6e-45	76.38	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G194150 PE=4 SV=1	[more]
A0A0D2TAB6_GOSRA	8.1e-36	67.19	Uncharacterized protein OS=Gossypium raimondii GN=B456_011G190000 PE=4 SV=1	[more]
A0A061EQ76_THECC	6.8e-35	65.89	Rapid alkalinization factor 1, putative OS=Theobroma cacao GN=TCM_021204 PE=4 SV...	[more]
G7JLH2_MEDTR	2.6e-34	69.23	RALF OS=Medicago truncatula GN=MTR_4g119860 PE=4 SV=2	[more]
A5BA80_VITVI	2.6e-34	64.46	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0060g02150 PE=4 SV=...	[more]

BLAST of CmaCh17G003240 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G16570.1	2.1e-35	59.42	rapid alkalinization factor 23	[more]
AT4G15800.1	1.4e-34	66.37	ralf-like 33	[more]
AT1G02900.1	6.8e-26	53.66	rapid alkalinization factor 1	[more]
AT3G05490.1	4.4e-25	50.39	ralf-like 22	[more]
AT2G33775.1	2.3e-18	67.74	ralf-like 19	[more]

BLAST of CmaCh17G003240 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450680\|ref\|XP_004143090.1\|	8.0e-45	76.38	PREDICTED: protein RALF-like 33 [Cucumis sativus]	[more]
gi\|659117160\|ref\|XP_008458453.1\|	5.2e-44	75.78	PREDICTED: protein RALF-like 33 [Cucumis melo]	[more]
gi\|823244996\|ref\|XP_012455175.1\|	1.2e-35	67.19	PREDICTED: protein RALF-like 33 [Gossypium raimondii]	[more]
gi\|763805740\|gb\|KJB72678.1\|	1.2e-35	67.19	hypothetical protein B456_011G190000 [Gossypium raimondii]	[more]
gi\|720043259\|ref\|XP_010269500.1\|	9.8e-35	64.46	PREDICTED: rapid alkalinization factor-like [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G003240.1	CmaCh17G003240.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 66..126 score: 1.9
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 3..127 score: 1.8
None	No IPR available	PANTHER	PTHR33136:SF5	PROTEIN RALF-LIKE 1-RELATED	coord: 3..127 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh17G003240	Cucsa.091080	Cucumber (Gy14) v1	cgycmaB0183
CmaCh17G003240	Cucsa.172250	Cucumber (Gy14) v1	cgycmaB0485
CmaCh17G003240	Cla009305	Watermelon (97103) v1	cmawmB358
CmaCh17G003240	Cla019924	Watermelon (97103) v1	cmawmB348
CmaCh17G003240	Cla021738	Watermelon (97103) v1	cmawmB344
CmaCh17G003240	Csa6G194150	Cucumber (Chinese Long) v2	cmacuB381
CmaCh17G003240	Csa2G031140	Cucumber (Chinese Long) v2	cmacuB353
CmaCh17G003240	MELO3C004224	Melon (DHL92) v3.5.1	cmameB341
CmaCh17G003240	MELO3C021331	Melon (DHL92) v3.5.1	cmameB313
CmaCh17G003240	ClCG02G015440	Watermelon (Charleston Gray)	cmawcgB317
CmaCh17G003240	ClCG06G005140	Watermelon (Charleston Gray)	cmawcgB328
CmaCh17G003240	CSPI02G04290	Wild cucumber (PI 183967)	cmacpiB357
CmaCh17G003240	CSPI06G14730	Wild cucumber (PI 183967)	cmacpiB385
CmaCh17G003240	CmoCh17G003110	Cucurbita moschata (Rifu)	cmacmoB353
CmaCh17G003240	CmoCh08G012000	Cucurbita moschata (Rifu)	cmacmoB365
CmaCh17G003240	Lsi03G010550	Bottle gourd (USVL1VR-Ls)	cmalsiB350
CmaCh17G003240	Lsi11G005880	Bottle gourd (USVL1VR-Ls)	cmalsiB344
CmaCh17G003240	Lsi09G014280	Bottle gourd (USVL1VR-Ls)	cmalsiB331
CmaCh17G003240	Cp4.1LG11g09060	Cucurbita pepo (Zucchini)	cmacpeB370
CmaCh17G003240	Cp4.1LG12g02690	Cucurbita pepo (Zucchini)	cmacpeB371
CmaCh17G003240	Cp4.1LG17g00620	Cucurbita pepo (Zucchini)	cmacpeB377
CmaCh17G003240	MELO3C004224.2	Melon (DHL92) v3.6.1	cmamedB397
CmaCh17G003240	MELO3C021331.2	Melon (DHL92) v3.6.1	cmamedB365
CmaCh17G003240	CsaV3_2G006630	Cucumber (Chinese Long) v3	cmacucB0421
CmaCh17G003240	CsaV3_6G017190	Cucumber (Chinese Long) v3	cmacucB0453
CmaCh17G003240	Cla97C06G114640	Watermelon (97103) v2	cmawmbB377
CmaCh17G003240	Cla97C02G041210	Watermelon (97103) v2	cmawmbB363
CmaCh17G003240	Cla97C05G087570	Watermelon (97103) v2	cmawmbB366
CmaCh17G003240	Bhi01G000714	Wax gourd	cmawgoB0473
CmaCh17G003240	Bhi12G001826	Wax gourd	cmawgoB0464
CmaCh17G003240	CsGy6G014610	Cucumber (Gy14) v2	cgybcmaB765
CmaCh17G003240	Carg05502	Silver-seed gourd	carcmaB0091
CmaCh17G003240	Carg18842	Silver-seed gourd	carcmaB0985

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh17G003240	CmaCh14G008930	Cucurbita maxima (Rimu)	cmacmaB246
CmaCh17G003240	CmaCh08G012260	Cucurbita maxima (Rimu)	cmacmaB373

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh17G003240	Cucurbita maxima (Rimu)	cmacmaB249
CmaCh17G003240	Cucurbita maxima (Rimu)	cmacmaB369
CmaCh17G003240	Cucumber (Gy14) v1	cgycmaB0714
CmaCh17G003240	Cucurbita moschata (Rifu)	cmacmoB350
CmaCh17G003240	Cucurbita moschata (Rifu)	cmacmoB362
CmaCh17G003240	Wild cucumber (PI 183967)	cmacpiB359
CmaCh17G003240	Cucumber (Chinese Long) v2	cmacuB355
CmaCh17G003240	Melon (DHL92) v3.5.1	cmameB342
CmaCh17G003240	Watermelon (Charleston Gray)	cmawcgB320
CmaCh17G003240	Cucurbita pepo (Zucchini)	cmacpeB403
CmaCh17G003240	Cucurbita pepo (Zucchini)	cmacpeB412
CmaCh17G003240	Bottle gourd (USVL1VR-Ls)	cmalsiB353
CmaCh17G003240	Cucumber (Gy14) v2	cgybcmaB345
CmaCh17G003240	Melon (DHL92) v3.6.1	cmamedB398
CmaCh17G003240	Silver-seed gourd	carcmaB0848
CmaCh17G003240	Cucumber (Chinese Long) v3	cmacucB0425