CSPI02G04290 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G04290
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Rapid alkalinization factor
Location	Chr2 : 3083971 .. 3085068 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTTTGTTATTTCTCCATGACGTTTCCAAAAAAAGGAATCTCATAGAATGGCATATTCGTAATTAAGTAACAAAATAAAGGGCATGTTTATAGACCACGTTCTAAATAAGTCTTTCAATTCTCCTTCTTCACACTGTGAACTTCAAAAGCAAAGAGACGAAGCACAACCCACCGACGATTCGCCGGAGCCACCGCCGGAGCTGAATAAACTCTCAATTCTGACTACAATACACTGACAAACGAAGTCAGACAAACTACGACCTTTTTCTTGCATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTCTTATCTTCAGCTGTTCCTCAACCACCGTCCACGCCGGTCTAGGGATCCAACACAGTCTTGCATGGATTCCTAATCAATCTACCTGTAAGGGCTCCATAGCGGAGTGTTTCGGGGGAGAGGAGTTCGAATTTGATTCAGAAATCAACCGCCGTATCTTGGCAACTAGCCAGTACATCAGCTACGGCGCTCTACGAAGGAACAACGTGCCGTGCTCAAGACGTGGTGCATCTTATTACAATTGTCAGCCCGGAGCTCAGGCCAATCCCTACAGCCGTGGATGCAACGCCATTACGAGGTGCAGGAGTTGAGTAATTTGTTTGTACTCTTTGTTTAATAATCCCTCTCTCCTTTTCAATGTTCATTGATCTTTTTCCCCCCTTTCTTTATCTTTTCTTTGATATGGCTATTGGCTTTGATTGTTATACAAATTGAAAAAGGGGATATTGATCATATAGTGTCGTTCTTGTTGTTGTAAGTGTTGAAAGTTGTTGTGTGTTAATTAATTATATATCTCTGCTCAATAATTTGGTAGTCTTTTAGTGGCTTGATTGTTCATCCTTCTTTCTGTTCCTTTTTCCATGGAAGGGAAAAGGAGGAATCGGCAAAGAAAATGAGGAAGAAAACAGACTGTAATTAGTTGAAATTAGGGAGAATCATGAGGATGAATTGATTGTTATTTTGAGTATAGTTCTCTCCTAGAAAATCTCAATGATTGTGTTTCGGGTCTTGTTTTTGGGTTTAGATGAACTTCACATGGTCATTTCTTT

mRNA sequence

ATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTCTTATCTTCAGCTGTTCCTCAACCACCGTCCACGCCGGTCTAGGGATCCAACACAGTCTTGCATGGATTCCTAATCAATCTACCTGTAAGGGCTCCATAGCGGAGTGTTTCGGGGGAGAGGAGTTCGAATTTGATTCAGAAATCAACCGCCGTATCTTGGCAACTAGCCAGTACATCAGCTACGGCGCTCTACGAAGGAACAACGTGCCGTGCTCAAGACGTGGTGCATCTTATTACAATTGTCAGCCCGGAGCTCAGGCCAATCCCTACAGCCGTGGATGCAACGCCATTACGAGGTGCAGGAGTTGA

Coding sequence (CDS)

ATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTCTTATCTTCAGCTGTTCCTCAACCACCGTCCACGCCGGTCTAGGGATCCAACACAGTCTTGCATGGATTCCTAATCAATCTACCTGTAAGGGCTCCATAGCGGAGTGTTTCGGGGGAGAGGAGTTCGAATTTGATTCAGAAATCAACCGCCGTATCTTGGCAACTAGCCAGTACATCAGCTACGGCGCTCTACGAAGGAACAACGTGCCGTGCTCAAGACGTGGTGCATCTTATTACAATTGTCAGCCCGGAGCTCAGGCCAATCCCTACAGCCGTGGATGCAACGCCATTACGAGGTGCAGGAGTTGA

Homology

[Close]

BLAST of CSPI02G04290 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 4.8e-31
Identity = 69/99 (69.70%), Postives = 78/99 (78.79%), Query Frame = 1


Query: 28  TVH---AGLGIQHSLAWIPNQSTCKGSIAECF---GGEEFEFDSEINRRILATSQYISYG 87
           TVH   A +  Q S  ++P +S C G+IAEC      EEFE DSEINRRILAT++YISYG
Sbjct: 17  TVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSEINRRILATTKYISYG 76

Query: 88  ALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           ALRRN VPCSRRGASYYNC+ GAQANPYSRGC+AITRCR
Sbjct: 77  ALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of CSPI02G04290 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.4e-30
Identity = 72/116 (62.07%), Postives = 82/116 (70.69%), Query Frame = 1


Query: 10  TFFLICAAVFLIFSCSSTTVHAGLGIQ-HSLAWIP---NQSTCKGSIAECFGGEEFEFDS 69
           T FL    + ++F  SS  V AG       +AW     N S C GSIAEC G EE E DS
Sbjct: 6   TLFLTLT-ILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAEEEEMDS 65

Query: 70  EINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 122
           EINRRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGC+ I RCRS
Sbjct: 66  EINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of CSPI02G04290 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 4.5e-29
Identity = 68/117 (58.12%), Postives = 83/117 (70.94%), Query Frame = 1


Query: 9   PTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWI-PNQST--CKGSIAECFGGEE-FEFD 68
           P+  ++C  +   F   +    +G     +  W+ P +S   CKGSI EC   EE FE D
Sbjct: 4   PSGLILCVLIGAFFISMAAAGDSG-----AYDWVMPARSGGGCKGSIGECIAEEEEFELD 63

Query: 69  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 122
           SE NRRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQANPYSRGC+AITRCRS
Sbjct: 64  SESNRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of CSPI02G04290 vs. Swiss-Prot
Match: RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.3e-28
Identity = 63/95 (66.32%), Postives = 72/95 (75.79%), Query Frame = 1


Query: 43  PNQSTCKGSIAEC-------------FGG----EEFEFDSEINRRILATSQYISYGALRR 102
           P ++ C+G+IAEC             +GG    EEFE DSEINRRILAT +YISYGALRR
Sbjct: 42  PFETECRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRR 101

Query: 103 NNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           N +PCSRRGASYYNC+ GAQANPYSRGC+AITRCR
Sbjct: 102 NTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 136

[Close]

BLAST of CSPI02G04290 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.5e-27
Identity = 65/116 (56.03%), Postives = 80/116 (68.97%), Query Frame = 1


Query: 7   NSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQ-STCKGSIAECFGGEE-FEFD 66
           N+   + + A + ++ S   +T   G  +    A   +  S C GSIAEC   EE  EFD
Sbjct: 3   NTRAIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           S+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GAQANPYSRGC+ ITRCR
Sbjct: 63  SDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

[Close]

BLAST of CSPI02G04290 vs. TrEMBL
Match: A0A0A0LFW9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G031140 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 2.3e-64
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 1


Query: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60
           MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120
           FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of CSPI02G04290 vs. TrEMBL
Match: A5BUA5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_037723 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 2.7e-36
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 1


Query: 7   NSPTFFLICA--AVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFD 66
           NS  F LICA  AV ++ + SS    +G  +Q  L WIP +S C+GSIAEC  GEEFE D
Sbjct: 3   NSSAFVLICAIMAVHMVLT-SSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           SEINRRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGC+AITRCR
Sbjct: 63  SEINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

[Close]

BLAST of CSPI02G04290 vs. TrEMBL
Match: F6HDF3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01490 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 2.7e-36
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 1


Query: 7   NSPTFFLICA--AVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFD 66
           NS  F LICA  AV ++ + SS    +G  +Q  L WIP +S C+GSIAEC  GEEFE D
Sbjct: 3   NSSAFVLICAIMAVHMVLT-SSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           SEINRRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGC+AITRCR
Sbjct: 63  SEINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

[Close]

BLAST of CSPI02G04290 vs. TrEMBL
Match: A0A061F478_THECC (Rapid alkalinization factor 1, putative OS=Theobroma cacao GN=TCM_026650 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.0e-35
Identity = 76/110 (69.09%), Postives = 87/110 (79.09%), Query Frame = 1


Query: 13  LICAAVFL-IFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFDSEINRRI 72
           +ICA +   + + ++ TV A    +  L WIP  S CKGSIAEC  GEEF+ DSEI+RRI
Sbjct: 9   VICAMIITAVVAATTRTVDASGEHERILGWIPTGSPCKGSIAECLAGEEFQLDSEISRRI 68

Query: 73  LATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 122
           LAT+QYISYGAL+RN VPCSRRGASYYNCQPGAQANPYSRGCN ITRCRS
Sbjct: 69  LATAQYISYGALQRNTVPCSRRGASYYNCQPGAQANPYSRGCNRITRCRS 118

[Close]

BLAST of CSPI02G04290 vs. TrEMBL
Match: A0A067JU03_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23313 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 5.0e-35
Identity = 80/117 (68.38%), Postives = 91/117 (77.78%), Query Frame = 1


Query: 12  FLICAAVFLIF---SCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECF----GGEEFEFD 71
           FL+C A+ +I    S +ST   +G   Q SL WIP +S+CKGSIAEC     GGEEFE D
Sbjct: 9   FLMCGAMLMILILMSGTSTVEASGDHHQQSLGWIPIRSSCKGSIAECLASDDGGEEFELD 68

Query: 72  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 122
           SEI+RRILATS YISYGAL+RN VPCSRRGASYYNCQ GAQANPY+RGC+ ITRCRS
Sbjct: 69  SEISRRILATSNYISYGALQRNTVPCSRRGASYYNCQNGAQANPYTRGCSTITRCRS 125

[Close]

BLAST of CSPI02G04290 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 135.2 bits (339), Expect = 2.7e-32
Identity = 69/99 (69.70%), Postives = 78/99 (78.79%), Query Frame = 1


Query: 28  TVH---AGLGIQHSLAWIPNQSTCKGSIAECF---GGEEFEFDSEINRRILATSQYISYG 87
           TVH   A +  Q S  ++P +S C G+IAEC      EEFE DSEINRRILAT++YISYG
Sbjct: 17  TVHFLFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEEEFEMDSEINRRILATTKYISYG 76

Query: 88  ALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           ALRRN VPCSRRGASYYNC+ GAQANPYSRGC+AITRCR
Sbjct: 77  ALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of CSPI02G04290 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 133.7 bits (335), Expect = 7.9e-32
Identity = 72/116 (62.07%), Postives = 82/116 (70.69%), Query Frame = 1


Query: 10  TFFLICAAVFLIFSCSSTTVHAGLGIQ-HSLAWIP---NQSTCKGSIAECFGGEEFEFDS 69
           T FL    + ++F  SS  V AG       +AW     N S C GSIAEC G EE E DS
Sbjct: 6   TLFLTLT-ILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAEEEEMDS 65

Query: 70  EINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 122
           EINRRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGC+ I RCRS
Sbjct: 66  EINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of CSPI02G04290 vs. TAIR10
Match: AT3G16570.1 (AT3G16570.1 rapid alkalinization factor 23)

HSP 1 Score: 127.1 bits (318), Expect = 7.4e-30
Identity = 63/95 (66.32%), Postives = 72/95 (75.79%), Query Frame = 1


Query: 43  PNQSTCKGSIAEC-------------FGG----EEFEFDSEINRRILATSQYISYGALRR 102
           P ++ C+G+IAEC             +GG    EEFE DSEINRRILAT +YISYGALRR
Sbjct: 42  PFETECRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRR 101

Query: 103 NNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           N +PCSRRGASYYNC+ GAQANPYSRGC+AITRCR
Sbjct: 102 NTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 136

[Close]

BLAST of CSPI02G04290 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 123.6 bits (309), Expect = 8.2e-29
Identity = 65/116 (56.03%), Postives = 80/116 (68.97%), Query Frame = 1


Query: 7   NSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQ-STCKGSIAECFGGEE-FEFD 66
           N+   + + A + ++ S   +T   G  +    A   +  S C GSIAEC   EE  EFD
Sbjct: 3   NTRAIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEEEMEFD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           S+I+RRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GAQANPYSRGC+ ITRCR
Sbjct: 63  SDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

[Close]

BLAST of CSPI02G04290 vs. TAIR10
Match: AT2G33775.1 (AT2G33775.1 ralf-like 19)

HSP 1 Score: 85.1 bits (209), Expect = 3.2e-17
Identity = 44/83 (53.01%), Postives = 54/83 (65.06%), Query Frame = 1


Query: 41  WIPNQSTCKGSIAECFGGE---EFEFDSEINRRILATSQ-YISYGALRRNNVPCSRRGAS 100
           W   +S   G    C G +   ++  DSE NRR LA  + YISYGALR+NNVPCSRRG S
Sbjct: 25  WTLTKSCVNGQ--GCIGEDGELDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRS 84

Query: 101 YYNCQPGAQANPYSRGCNAITRC 120
           YY+C+   +ANPY RGC+ IT C
Sbjct: 85  YYDCKKRKRANPYRRGCSVITHC 105

[Close]

BLAST of CSPI02G04290 vs. NCBI nr
Match: gi|700205836|gb|KGN60955.1| (hypothetical protein Csa_2G031140 [Cucumis sativus])

HSP 1 Score: 252.7 bits (644), Expect = 3.3e-64
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 1


Query: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60
           MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120
           FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of CSPI02G04290 vs. NCBI nr
Match: gi|659070254|ref|XP_008454184.1| (PREDICTED: protein RALF-like 33 [Cucumis melo])

HSP 1 Score: 243.4 bits (620), Expect = 2.0e-61
Identity = 117/121 (96.69%), Postives = 119/121 (98.35%), Query Frame = 1


Query: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60
           MAMAGFNSPTFFLI AAVFLIFSCSST VHAG+GIQ+SLAWIPNQSTCKGSIAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLISAAVFLIFSCSSTAVHAGIGIQNSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120
           FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of CSPI02G04290 vs. NCBI nr
Match: gi|147862659|emb|CAN83593.1| (hypothetical protein VITISV_037723 [Vitis vinifera])

HSP 1 Score: 159.5 bits (402), Expect = 3.8e-36
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 1


Query: 7   NSPTFFLICA--AVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFD 66
           NS  F LICA  AV ++ + SS    +G  +Q  L WIP +S C+GSIAEC  GEEFE D
Sbjct: 3   NSSAFVLICAIMAVHMVLT-SSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           SEINRRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGC+AITRCR
Sbjct: 63  SEINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

[Close]

BLAST of CSPI02G04290 vs. NCBI nr
Match: gi|225432308|ref|XP_002273386.1| (PREDICTED: protein RALF-like 33 [Vitis vinifera])

HSP 1 Score: 159.5 bits (402), Expect = 3.8e-36
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 1


Query: 7   NSPTFFLICA--AVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFD 66
           NS  F LICA  AV ++ + SS    +G  +Q  L WIP +S C+GSIAEC  GEEFE D
Sbjct: 3   NSSAFVLICAIMAVHMVLT-SSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMD 62

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           SEINRRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGC+AITRCR
Sbjct: 63  SEINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 117

[Close]

BLAST of CSPI02G04290 vs. NCBI nr
Match: gi|297736875|emb|CBI26076.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 159.5 bits (402), Expect = 3.8e-36
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 1


Query: 7   NSPTFFLICA--AVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEEFEFD 66
           NS  F LICA  AV ++ + SS    +G  +Q  L WIP +S C+GSIAEC  GEEFE D
Sbjct: 37  NSSAFVLICAIMAVHMVLT-SSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMD 96

Query: 67  SEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCR 121
           SEINRRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGC+AITRCR
Sbjct: 97  SEINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCR 151

The following BLAST results are available for this feature:

BLAST of CSPI02G04290 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF33_ARATH	4.8e-31	69.70	Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1	[more]
RLF1_ARATH	1.4e-30	62.07	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RALF_TOBAC	4.5e-29	58.12	Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1	[more]
RLF23_ARATH	1.3e-28	66.32	Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1	[more]
RLF22_ARATH	1.5e-27	56.03	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]

BLAST of CSPI02G04290 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW9_CUCSA	2.3e-64	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G031140 PE=4 SV=1	[more]
A5BUA5_VITVI	2.7e-36	68.10	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_037723 PE=4 SV=1	[more]
F6HDF3_VITVI	2.7e-36	68.10	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01490 PE=4 SV=...	[more]
A0A061F478_THECC	1.0e-35	69.09	Rapid alkalinization factor 1, putative OS=Theobroma cacao GN=TCM_026650 PE=4 SV...	[more]
A0A067JU03_JATCU	5.0e-35	68.38	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23313 PE=4 SV=1	[more]

BLAST of CSPI02G04290 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G15800.1	2.7e-32	69.70	ralf-like 33	[more]
AT1G02900.1	7.9e-32	62.07	rapid alkalinization factor 1	[more]
AT3G16570.1	7.4e-30	66.32	rapid alkalinization factor 23	[more]
AT3G05490.1	8.2e-29	56.03	ralf-like 22	[more]
AT2G33775.1	3.2e-17	53.01	ralf-like 19	[more]

BLAST of CSPI02G04290 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700205836\|gb\|KGN60955.1\|	3.3e-64	100.00	hypothetical protein Csa_2G031140 [Cucumis sativus]	[more]
gi\|659070254\|ref\|XP_008454184.1\|	2.0e-61	96.69	PREDICTED: protein RALF-like 33 [Cucumis melo]	[more]
gi\|147862659\|emb\|CAN83593.1\|	3.8e-36	68.10	hypothetical protein VITISV_037723 [Vitis vinifera]	[more]
gi\|225432308\|ref\|XP_002273386.1\|	3.8e-36	68.10	PREDICTED: protein RALF-like 33 [Vitis vinifera]	[more]
gi\|297736875\|emb\|CBI26076.3\|	3.8e-36	68.10	unnamed protein product [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005622	intracellular
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0004871	signal transducer activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G04290.1	CSPI02G04290.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 59..120 score: 1.9
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 1..121 score: 4.8
None	No IPR available	PANTHER	PTHR33136:SF5	PROTEIN RALF-LIKE 1-RELATED	coord: 1..121 score: 4.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G04290	Cla009305	Watermelon (97103) v1	cpiwmB180
CSPI02G04290	Cla019924	Watermelon (97103) v1	cpiwmB149
CSPI02G04290	Csa2G031140	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G04290	MELO3C004224	Melon (DHL92) v3.5.1	cpimeB139
CSPI02G04290	ClCG06G005140	Watermelon (Charleston Gray)	cpiwcgB161
CSPI02G04290	ClCG02G015440	Watermelon (Charleston Gray)	cpiwcgB128
CSPI02G04290	Lsi11G005880	Bottle gourd (USVL1VR-Ls)	cpilsiB096
CSPI02G04290	MELO3C004224.2	Melon (DHL92) v3.6.1	cpimedB132
CSPI02G04290	CsaV3_2G006630	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G04290	Cla97C06G114640	Watermelon (97103) v2	cpiwmbB154
CSPI02G04290	Cla97C02G041210	Watermelon (97103) v2	cpiwmbB120
CSPI02G04290	Cucsa.091080	Cucumber (Gy14) v1	cgycpiB097
CSPI02G04290	CmaCh05G011240	Cucurbita maxima (Rimu)	cmacpiB796
CSPI02G04290	CmaCh17G003240	Cucurbita maxima (Rimu)	cmacpiB357
CSPI02G04290	CmaCh08G012260	Cucurbita maxima (Rimu)	cmacpiB900
CSPI02G04290	CmaCh12G010600	Cucurbita maxima (Rimu)	cmacpiB174
CSPI02G04290	CmoCh17G003110	Cucurbita moschata (Rifu)	cmocpiB349
CSPI02G04290	CmoCh12G010730	Cucurbita moschata (Rifu)	cmocpiB159
CSPI02G04290	CmoCh05G011360	Cucurbita moschata (Rifu)	cmocpiB786
CSPI02G04290	Cp4.1LG07g08990	Cucurbita pepo (Zucchini)	cpecpiB821
CSPI02G04290	Cp4.1LG11g09060	Cucurbita pepo (Zucchini)	cpecpiB097
CSPI02G04290	CsGy2G004400	Cucumber (Gy14) v2	cgybcpiB056
CSPI02G04290	Carg05502	Silver-seed gourd	carcpiB0073
CSPI02G04290	Carg26765	Silver-seed gourd	carcpiB0775
CSPI02G04290	Carg23292	Silver-seed gourd	carcpiB0916

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G04290	Cucurbita pepo (Zucchini)	cpecpiB100
CSPI02G04290	Bottle gourd (USVL1VR-Ls)	cpilsiB134
CSPI02G04290	Melon (DHL92) v3.6.1	cpimedB109
CSPI02G04290	Cucumber (Chinese Long) v3	cpicucB078
CSPI02G04290	Wax gourd	cpiwgoB129
CSPI02G04290	Wild cucumber (PI 183967)	cpicpiB013
CSPI02G04290	Cucurbita maxima (Rimu)	cmacpiB800
CSPI02G04290	Cucurbita moschata (Rifu)	cmocpiB787
CSPI02G04290	Cucumber (Chinese Long) v2	cpicuB063
CSPI02G04290	Melon (DHL92) v3.5.1	cpimeB113
CSPI02G04290	Watermelon (97103) v1	cpiwmB120