Carg23292 (gene) Silver-seed gourd

NameCarg23292
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRapid ALkalinization Factor
LocationCucurbita_argyrosperma_scaffold_231 : 225959 .. 227341 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGCCGGATTCAATTCCTCGACATTCTTCCTGATCTGCGCCGCTGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTTCACGGAGGCCTCGGGATCCAACACAGCCTCGGATGGATTCCTAACCAATCGACCTGCAAAGGCACAATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGACGTATCTTGGCCACCAGTCAGTACATCAGTTACGGCGCTCTGCGGAGGAACACCGTGCCGTGCTCGAGACGCGGTGCATCATACTACAACTGCCAGCCCGGAGCTCAGGCCAACCCTTACAGCCGTGGATGCAGCGCCATTACAAGGTGCAGGAGTTGAGTAATTTGTTTGTACTCTCTGTTTAATAATCTCTTTCTCTCCTTTCAATGTTCATTGATCTTTATTTTTTTCTTCTTTTTTTCCTCTTTTTTGAGATTGCTTTGGTTGTTATACAAATTGAAAAGGGGAGATTGATTATATAATGTCGTCGTTGTCGTTATTGTAAGTGATGAAAATTGTTGTGCTCAATTAATTATATATATATATCTTCTCAATAATGTGATATTCTATTCCTGGCTGGGATTTCTTTCCTCTTTCTTTCCTTTTGTCGATTGAAGGGAAAAGAGATTCTGCAAAGAGATTGAAGAACAAGAAAGGCTGTAATGGCTGGAATTAAGGAGAATTATGGCTCCCATCATGAGATAAGAGTAAGAACAAATTCATCAGTTGTTATAATTAGTATATTTCTCTTATAGAAACTGTGAAAGATTGTGTTTCTGATGCTGTTTTTGGGTTTAGATTAACTTTACAATGCCAATTTCTTTCCATTTTGGGTTTTGATCGGCTGACCAAACCATTTCCTTTCGTTTACTTGTTTTTTCTCTCTCTGTTCTCATCTGTTTATGGTAAAGAATCTTGAACTCTTTAGGAAAAATATTTAGAGAGGGAAGTTTTCCTTGAGAAATCATACCATACTACTGATTTGATTCTTGGTGCTCTTGAATTGGCTATCATGTTGAGGTTGGGTACTTTTCTTAGAACTTTCATTATGTTGACAAGAGAAGTAAATATCAAATATTTGTAACCATATGTATATGTGTGTGCATTGATTATGGTTTTACTTAGTTTTAGTATCATATTGAGGATAATTATGCATTCTAGAAGTAAAACTACTTCCATGGATTGCCCTAATGATCGAAAGGGCGAACCAGATGTTCTTGAGATGTATATGTCTGTGCATTGATTATGGTTTCAAGCTATGGTGTTGGTGGCTATCTATCTAGGATTTCCCGTTGTTATCTGAGGTTTGTTGAGATGTACATGATGTGGATGGGACTCATATTATATATTAGTATA

mRNA sequence

ATGGCAATGGCCGGATTCAATTCCTCGACATTCTTCCTGATCTGCGCCGCTGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTTCACGGAGGCCTCGGGATCCAACACAGCCTCGGATGGATTCCTAACCAATCGACCTGCAAAGGCACAATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGACGTATCTTGGCCACCAGTCAGTACATCAGTTACGGCGCTCTGCGGAGGAACACCGTGCCGTGCTCGAGACGCGGTGCATCATACTACAACTGCCAGCCCGGAGCTCAGGCCAACCCTTACAGCCGTGGATGCAGCGCCATTACAAGGGAAAAGAGATTCTGCAAAGAGATTGAAGAACAAGAAAGGCTGTAATGGCTGGAATTAAGGAGAATTATGGCTCCCATCATGAGATAAGAGTAAGAACAAATTCATCAGTTGTTATAATTAGTATATTTCTCTTATAGAAACTGTGAAAGATTGTGTTTCTGATGCTGTTTTTGGGTTTAGATTAACTTTACAATGCCAATTTCTTTCCATTTTGGGTTTTGATCGGCTGACCAAACCATTTCCTTTCGTTTACTTGTTTTTTCTCTCTCTGTTCTCATCTGTTTATGGTAAAGAATCTTGAACTCTTTAGGAAAAATATTTAGAGAGGGAAGTTTTCCTTGAGAAATCATACCATACTACTGATTTGATTCTTGGTGCTCTTGAATTGGCTATCATGTTGAGGTTGGGTACTTTTCTTAGAACTTTCATTATGTTGACAAGAGAAGTAAATATCAAATATTTGTAACCATATGTATATGTGTGTGCATTGATTATGGTTTTACTTAGTTTTAGTATCATATTGAGGATAATTATGCATTCTAGAAGTAAAACTACTTCCATGGATTGCCCTAATGATCGAAAGGGCGAACCAGATGTTCTTGAGATGTATATGTCTGTGCATTGATTATGGTTTCAAGCTATGGTGTTGGTGGCTATCTATCTAGGATTTCCCGTTGTTATCTGAGGTTTGTTGAGATGTACATGATGTGGATGGGACTCATATTATATATTAGTATA

Coding sequence (CDS)

ATGGCAATGGCCGGATTCAATTCCTCGACATTCTTCCTGATCTGCGCCGCTGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTTCACGGAGGCCTCGGGATCCAACACAGCCTCGGATGGATTCCTAACCAATCGACCTGCAAAGGCACAATAGCGGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGACGTATCTTGGCCACCAGTCAGTACATCAGTTACGGCGCTCTGCGGAGGAACACCGTGCCGTGCTCGAGACGCGGTGCATCATACTACAACTGCCAGCCCGGAGCTCAGGCCAACCCTTACAGCCGTGGATGCAGCGCCATTACAAGGGAAAAGAGATTCTGCAAAGAGATTGAAGAACAAGAAAGGCTGTAA

Protein sequence

MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITREKRFCKEIEEQERL
BLAST of Carg23292 vs. NCBI nr
Match: XP_022937540.1 (protein RALF-like 33 [Cucurbita moschata])

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-61
Identity = 118/118 (100.00%), Postives = 118/118 (100.00%), Query Frame = 0

Query: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60
           MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE
Sbjct: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR
Sbjct: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 118

BLAST of Carg23292 vs. NCBI nr
Match: XP_022966015.1 (protein RALF-like 33 [Cucurbita maxima] >XP_023537429.1 protein RALF-like 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242.3 bits (617), Expect = 9.4e-61
Identity = 117/118 (99.15%), Postives = 117/118 (99.15%), Query Frame = 0

Query: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60
           MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGI HSLGWIPNQSTCKGTIAECFGGEE
Sbjct: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIHHSLGWIPNQSTCKGTIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR
Sbjct: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 118

BLAST of Carg23292 vs. NCBI nr
Match: KGN60955.1 (hypothetical protein Csa_2G031140 [Cucumis sativus])

HSP 1 Score: 228.8 bits (582), Expect = 1.1e-56
Identity = 108/118 (91.53%), Postives = 113/118 (95.76%), Query Frame = 0

Query: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60
           MAMAGFNS TFFLICAAVF++FSCSST VH GLGIQHSL WIPNQSTCKG+IAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FEFDSEI+RRILATSQYISYGALRRN VPCSRRGASYYNCQPGAQANPYSRGC+AITR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITR 118

BLAST of Carg23292 vs. NCBI nr
Match: XP_022150733.1 (protein RALF-like 33 [Momordica charantia])

HSP 1 Score: 220.7 bits (561), Expect = 2.9e-54
Identity = 107/118 (90.68%), Postives = 113/118 (95.76%), Query Frame = 0

Query: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60
           MAMAGF+S T FLICAAVFVVF+CSSTAVHGGLGI HSL WIP+QS+CKG+IAECFGGEE
Sbjct: 1   MAMAGFHSPT-FLICAAVFVVFTCSSTAVHGGLGIHHSLAWIPDQSSCKGSIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FEFDSEISRRILATSQYISYGAL+RNTVPCSRRGASYYNCQPGAQANPY RGCSAITR
Sbjct: 61  FEFDSEISRRILATSQYISYGALKRNTVPCSRRGASYYNCQPGAQANPYGRGCSAITR 117

BLAST of Carg23292 vs. NCBI nr
Match: XP_022999036.1 (protein RALF-like 33 [Cucurbita maxima])

HSP 1 Score: 218.4 bits (555), Expect = 1.5e-53
Identity = 106/116 (91.38%), Postives = 109/116 (93.97%), Query Frame = 0

Query: 3   MAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEEFE 62
           MAGFNS TF LICA VFVVFS SSTAV G LGIQHS+ WIPNQ+TCKG+IAECFGGEEFE
Sbjct: 1   MAGFNSPTFLLICATVFVVFSFSSTAVLGSLGIQHSVAWIPNQATCKGSIAECFGGEEFE 60

Query: 63  FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR
Sbjct: 61  FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 116

BLAST of Carg23292 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 130.2 bits (326), Expect = 9.5e-31
Identity = 72/122 (59.02%), Postives = 86/122 (70.49%), Query Frame = 0

Query: 3   MAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAEC---FGGE 62
           M G ++    +I A + V F      +   +  Q S  ++P +S C GTIAEC      E
Sbjct: 1   MRGLSTKPVAIIIAILTVHF------LFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEE 60

Query: 63  EFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRE 122
           EFE DSEI+RRILAT++YISYGALRRNTVPCSRRGASYYNC+ GAQANPYSRGCSAITR 
Sbjct: 61  EFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRC 116

BLAST of Carg23292 vs. TAIR10
Match: AT3G16570.1 (rapid alkalinization factor 23)

HSP 1 Score: 125.6 bits (314), Expect = 2.3e-29
Identity = 64/96 (66.67%), Postives = 73/96 (76.04%), Query Frame = 0

Query: 43  PNQSTCKGTIAEC-------------FG----GEEFEFDSEISRRILATSQYISYGALRR 102
           P ++ C+GTIAEC             +G    GEEFE DSEI+RRILAT +YISYGALRR
Sbjct: 42  PFETECRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRR 101

Query: 103 NTVPCSRRGASYYNCQPGAQANPYSRGCSAITREKR 122
           NT+PCSRRGASYYNC+ GAQANPYSRGCSAITR +R
Sbjct: 102 NTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 137

BLAST of Carg23292 vs. TAIR10
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 125.2 bits (313), Expect = 3.1e-29
Identity = 70/117 (59.83%), Postives = 80/117 (68.38%), Query Frame = 0

Query: 8   SSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWI------PNQSTCKGTIAECFGGEEF 67
           S T FL    + VVF  SS  V  G    + LG +       N S C G+IAEC G EE 
Sbjct: 4   SFTLFL-TLTILVVFIISSPPVQAGFA--NDLGGVAWATTGDNGSGCHGSIAECIGAEEE 63

Query: 68  EFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           E DSEI+RRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGCS I R
Sbjct: 64  EMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIAR 117

BLAST of Carg23292 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 121.3 bits (303), Expect = 4.4e-28
Identity = 59/77 (76.62%), Postives = 65/77 (84.42%), Query Frame = 0

Query: 46  STCKGTIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGA 105
           S C G+IAEC    EE EFDS+ISRRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GA
Sbjct: 43  SGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGA 102

Query: 106 QANPYSRGCSAITREKR 122
           QANPYSRGCS ITR +R
Sbjct: 103 QANPYSRGCSTITRCRR 119

BLAST of Carg23292 vs. TAIR10
Match: AT2G33775.1 (ralf-like 19)

HSP 1 Score: 78.2 bits (191), Expect = 4.3e-15
Identity = 38/62 (61.29%), Postives = 46/62 (74.19%), Query Frame = 0

Query: 57  GGEEFEFDSEISRRILATSQ-YISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSA 116
           G  ++  DSE +RR LA  + YISYGALR+N VPCSRRG SYY+C+   +ANPY RGCS 
Sbjct: 42  GELDYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSV 101

Query: 117 IT 118
           IT
Sbjct: 102 IT 103

BLAST of Carg23292 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.7e-29
Identity = 72/122 (59.02%), Postives = 86/122 (70.49%), Query Frame = 0

Query: 3   MAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAEC---FGGE 62
           M G ++    +I A + V F      +   +  Q S  ++P +S C GTIAEC      E
Sbjct: 1   MRGLSTKPVAIIIAILTVHF------LFAAVTSQSSGDFVPIESKCNGTIAECSLSTAEE 60

Query: 63  EFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRE 122
           EFE DSEI+RRILAT++YISYGALRRNTVPCSRRGASYYNC+ GAQANPYSRGCSAITR 
Sbjct: 61  EFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRC 116

BLAST of Carg23292 vs. Swiss-Prot
Match: sp|Q9LUS7|RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.2e-28
Identity = 64/96 (66.67%), Postives = 73/96 (76.04%), Query Frame = 0

Query: 43  PNQSTCKGTIAEC-------------FG----GEEFEFDSEISRRILATSQYISYGALRR 102
           P ++ C+GTIAEC             +G    GEEFE DSEI+RRILAT +YISYGALRR
Sbjct: 42  PFETECRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRR 101

Query: 103 NTVPCSRRGASYYNCQPGAQANPYSRGCSAITREKR 122
           NT+PCSRRGASYYNC+ GAQANPYSRGCSAITR +R
Sbjct: 102 NTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 137

BLAST of Carg23292 vs. Swiss-Prot
Match: sp|Q9SRY3|RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.5e-28
Identity = 70/117 (59.83%), Postives = 80/117 (68.38%), Query Frame = 0

Query: 8   SSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWI------PNQSTCKGTIAECFGGEEF 67
           S T FL    + VVF  SS  V  G    + LG +       N S C G+IAEC G EE 
Sbjct: 4   SFTLFL-TLTILVVFIISSPPVQAGFA--NDLGGVAWATTGDNGSGCHGSIAECIGAEEE 63

Query: 68  EFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           E DSEI+RRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGCS I R
Sbjct: 64  EMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIAR 117

BLAST of Carg23292 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.1e-27
Identity = 57/72 (79.17%), Postives = 64/72 (88.89%), Query Frame = 0

Query: 48  CKGTIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQA 107
           CKG+I EC    EEFE DSE +RRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQA
Sbjct: 41  CKGSIGECIAEEEEFELDSESNRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQA 100

Query: 108 NPYSRGCSAITR 119
           NPYSRGCSAITR
Sbjct: 101 NPYSRGCSAITR 112

BLAST of Carg23292 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 7.9e-27
Identity = 59/77 (76.62%), Postives = 65/77 (84.42%), Query Frame = 0

Query: 46  STCKGTIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGA 105
           S C G+IAEC    EE EFDS+ISRRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GA
Sbjct: 43  SGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGA 102

Query: 106 QANPYSRGCSAITREKR 122
           QANPYSRGCS ITR +R
Sbjct: 103 QANPYSRGCSTITRCRR 119

BLAST of Carg23292 vs. TrEMBL
Match: tr|A0A0A0LFW9|A0A0A0LFW9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G031140 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.1e-57
Identity = 108/118 (91.53%), Postives = 113/118 (95.76%), Query Frame = 0

Query: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60
           MAMAGFNS TFFLICAAVF++FSCSST VH GLGIQHSL WIPNQSTCKG+IAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           FEFDSEI+RRILATSQYISYGALRRN VPCSRRGASYYNCQPGAQANPYSRGC+AITR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITR 118

BLAST of Carg23292 vs. TrEMBL
Match: tr|A0A2C9VHY5|A0A2C9VHY5_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G118000 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.7e-40
Identity = 86/110 (78.18%), Postives = 95/110 (86.36%), Query Frame = 0

Query: 12  FLICAAVFVVFSC-SSTAVHGGLGIQHSLGWIPNQSTCKGTIAECF--GGEEFEFDSEIS 71
           F+ICAA+F++ S  +ST VHGG   QH LGWIP +S+CKG+IAEC    GEEFE DSEIS
Sbjct: 9   FMICAALFILISMNASTTVHGGGDHQHQLGWIPIRSSCKGSIAECLASNGEEFELDSEIS 68

Query: 72  RRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           RRILATS YISYGAL+RNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR
Sbjct: 69  RRILATSSYISYGALQRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 118

BLAST of Carg23292 vs. TrEMBL
Match: tr|A0A2I4GPT3|A0A2I4GPT3_9ROSI (protein RALF-like 33 OS=Juglans regia OX=51240 GN=LOC109009756 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-36
Identity = 86/117 (73.50%), Postives = 93/117 (79.49%), Query Frame = 0

Query: 3   MAG-FNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEEF 62
           MAG F SS  F ICA V +VFS SS+ VH G    H LG+IP  S+CKGT+AEC   EEF
Sbjct: 1   MAGIFYSSAVFGICALV-LVFSSSSSTVHAGGLSHHQLGFIPAGSSCKGTVAECLAAEEF 60

Query: 63  EFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           E  SEISRRILATS YISYGAL+RNTVPCSRRGASYYNCQ GAQANPYSRGCS+ITR
Sbjct: 61  ELGSEISRRILATSGYISYGALQRNTVPCSRRGASYYNCQGGAQANPYSRGCSSITR 116

BLAST of Carg23292 vs. TrEMBL
Match: tr|A0A2C9VBI5|A0A2C9VBI5_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G169900 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-36
Identity = 81/119 (68.07%), Postives = 95/119 (79.83%), Query Frame = 0

Query: 3   MAGFNSSTFFLICAAVFV-VFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFG--GE 62
           MA   +S+F +  AA+F+ +   +S+ VHG     H LGWIP +S+CKG+IAEC    GE
Sbjct: 1   MANLLASSFLVCIAALFISILMSTSSTVHGSGEHLHHLGWIPIRSSCKGSIAECLAIDGE 60

Query: 63  EFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITR 119
           EFE DSEISRRILATS YISYGA++RNTVPCSRRGASYYNCQPGAQANPY+RGCSAITR
Sbjct: 61  EFELDSEISRRILATSNYISYGAMQRNTVPCSRRGASYYNCQPGAQANPYTRGCSAITR 119

BLAST of Carg23292 vs. TrEMBL
Match: tr|A5BUA5|A5BUA5_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_037723 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-35
Identity = 79/116 (68.10%), Postives = 93/116 (80.17%), Query Frame = 0

Query: 7   NSSTFFLICAAVFVVFSCSSTAV-HGGLGIQHSLGWIPNQSTCKGTIAECFGGEEFEFDS 66
           NSS F LICA + V    +S+AV   G  +Q  LGWIP +S C+G+IAEC  GEEFE DS
Sbjct: 3   NSSAFVLICAIMAVHMVLTSSAVDFSGDHLQFQLGWIPARSACQGSIAECLAGEEFEMDS 62

Query: 67  EISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITREKR 122
           EI+RRILA+ +YISYGAL RN+VPCSRRGASYYNC+PGAQANPY+RGCSAITR +R
Sbjct: 63  EINRRILASKRYISYGALSRNSVPCSRRGASYYNCRPGAQANPYTRGCSAITRCRR 118

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022937540.12.5e-61100.00protein RALF-like 33 [Cucurbita moschata][more]
XP_022966015.19.4e-6199.15protein RALF-like 33 [Cucurbita maxima] >XP_023537429.1 protein RALF-like 33 [Cu... [more]
KGN60955.11.1e-5691.53hypothetical protein Csa_2G031140 [Cucumis sativus][more]
XP_022150733.12.9e-5490.68protein RALF-like 33 [Momordica charantia][more]
XP_022999036.11.5e-5391.38protein RALF-like 33 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G15800.19.5e-3159.02ralf-like 33[more]
AT3G16570.12.3e-2966.67rapid alkalinization factor 23[more]
AT1G02900.13.1e-2959.83rapid alkalinization factor 1[more]
AT3G05490.14.4e-2876.62ralf-like 22[more]
AT2G33775.14.3e-1561.29ralf-like 19[more]
Match NameE-valueIdentityDescription
sp|Q8L9P8|RLF33_ARATH1.7e-2959.02Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1[more]
sp|Q9LUS7|RLF23_ARATH4.2e-2866.67Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV... [more]
sp|Q9SRY3|RLF1_ARATH5.5e-2859.83Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1[more]
sp|Q945T0|RALF_TOBAC6.1e-2779.17Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1[more]
sp|Q9MA62|RLF22_ARATH7.9e-2776.62Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LFW9|A0A0A0LFW9_CUCSA7.1e-5791.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G031140 PE=4 SV=1[more]
tr|A0A2C9VHY5|A0A2C9VHY5_MANES2.7e-4078.18Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G118000 PE=4 SV=... [more]
tr|A0A2I4GPT3|A0A2I4GPT3_9ROSI1.1e-3673.50protein RALF-like 33 OS=Juglans regia OX=51240 GN=LOC109009756 PE=4 SV=1[more]
tr|A0A2C9VBI5|A0A2C9VBI5_MANES1.8e-3668.07Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G169900 PE=4 SV=... [more]
tr|A5BUA5|A5BUA5_VITVI1.2e-3568.10Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_037723 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008801RALF
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23292-RACarg23292-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 59..118
e-value: 5.1E-29
score: 100.8
NoneNo IPR availablePANTHERPTHR33136FAMILY NOT NAMEDcoord: 9..118
NoneNo IPR availablePANTHERPTHR33136:SF4SUBFAMILY NOT NAMEDcoord: 9..118