Cucsat.G6901 (gene) Cucumber (B10) v3

Overview
NameCucsat.G6901
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPseudouridine synthase
Locationctg1522: 100693 .. 105623 (+)
RNA-Seq ExpressionCucsat.G6901
SyntenyCucsat.G6901
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAAATTGGAGGTTGTTATTGCAATTCTCCATACTGGTTGTTTAAATTTATTTAAAAATAAATAAAAATGAAAAACAAAAGAAAAACCCAATTTTCCCGGTAAGAAAACAGACAGCCGATTAGTGAAAACCCAGAAACGAAACTATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTAGCGTCATTTTCCATAGAGAAGCTACATTTGCTAACCTTTTTTTTTTTCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTATGATTATCTAGTGGTCTGTAATCGGAGTGGTGAATTATGTTCAGCCTATATTTACTGTTTTTGTTCCATATTTGAGTTCTTAATTTGTGTGTTCATTTGCAACTTCTATATATTTTTTAAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTATTTGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCACGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCCTTTTTAAGCTTCCTTGTTACTGCTCATCTGAAGATTAAAGCAGACAATTCAAGTTTGGTGCTTCAAAAGTTTGCTACTCAAACACTTGTAATATCATGTTTAACTTCTAAGTTTTTTTCATTGGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTATGTACATAATTATTTTTCAGTGATAGAAAATATTTAAATATCACTGATTTTGGGCGTTCCTAACGCAAAGTTGAAACACCGAATGTTTCGCAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTAGTCTAAATGTCTTTCTCCTTAGGCTGTTTCAAATGACCAAAAAAAAAATTATATTACAATCTATCAATAGTCAGTTTAGAACATAAATTATATTCCCTAACACCACGCATATGTGCAAAGACATACATTTCTTTTGTTTGTAAAAGATTTCTCTATGATACTGCTACTGTTTTCCAGTCTTCATATGTGCATATGTAAAATAAAATTTCAATATAACGAGTCAGAGTTGCACCTCGAGCATACAAGCAGCCACATTTACCATTCCACATCCACTGCAATTTCAGTTCTTATCTTTTTTTAAAAAAAATTGAAATGCAGTTTTATTTTACTCGGTCTTAGGATTGAATATTTGTGAAGTAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAACAAATCTTTAAGAGCTCATTTAGTCCGTTCTTATTTCATTTACTATCCATTTTTTGAGAAATATGTGATTTTGACCTTTCTTCTTTATTTTATTTTATTTATTCTTTTCTAAAATAGTTTATAACTTTCATCTCTTATTTCTAATGTTTTGAAACTAAATGGAATTTATATTTGTATTATTTTCCTCTAATAATAATTGTATGCAAAATATCAAAACATTAATTTGACTATTCAATTCCAATTTTTCGTTTAAATTTCCACTTTTTGAAAAAGAAACGTTGTAGAGAAAAATAAATGATAACAACTGCTAACCACATTTTTGTTCTTTTTTAATAAAAATGGAGAAAAGCAAGAAATGGTATAGAAACATGTTTTCACTTTTTACAATAAAAGAGACTAATGAATCTTTACTTGTTCATTCCTTCCGTAAACAACTAATTCAAGATCACTGAATATTTTGTAGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAAGTTGAGCCAAGCTTTTTGCTTGGTAATCGGTTATTTTACAGATTTAATATCCATCCATCATCATGAAGAACAATATAAAAAATGTCCCGAGGGATACAATTGACACAACCAACCTCATTCGTAGCAACCTAGGTAGTTAATACGTGTCACCTTTATGCCTATTTTCTGGGCGCCCCCTTGCCATGTTATAGCTAATACCGGGTCTTCTCTATTTACTAGCTATTTTTCACCTGGTTCTTGTGTTTGGAGTGACTATTTGTTGGTTTAGTTTCCTTTTGTACTTCATCGCTAACTTATTCTACTAAACTTAACACAAAAATAAGTTGTTTAATGATGGGTGAAAATTTAGCAATACATAAAATATATTTTCTTATTATTTAAACGATGGTTTTATACATGAAAACATTGGAGACATGATAGATATAACATAATAACTAAATAGCAGACAAACAAGCATAGAAGCTCTCCTTTTACTTGCTTCTCTTATAAATAGTAACGAATAGGGACGTTTGTTCTATCTATACAAATGACATAATGGGGATAAATTCCCTGAACAATTTTGAACTCTATATCATGTGGAAATTTCGCATCATATCTTGTGGATTAATCTTATGCCGGTCTGATGGTAGATAGGGCAAATTTGAAATAATTTTGAGCTTCACAAAATATATTAGATGTTGAACAATGGTTACTACCATAGAATCTAGGTGAGATGTGGTTTTTACGGTGCCCCAGTTAACCTATAATAGAGTGTTGTCATAAAAGTGGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCGTCAAATGACTCGAAAATGAGATATCAAGAAGGTACATTAGGACATACAGCCATTTAAGTTGTGATGTCTGAAGCACAAATTTAAGTTCTGGGTAACTTACATATCTTGTTTATTAGATTCATTTAATTGTTGAAATTTAAAGTATTTTGTCACACATTCCCAGACGTTGAATTCCACGTATTGTTAATCTCTGCCATTAACTTCGATTATTGAAACCATTTAGAATCAATATTGTATATACGGGTAGTTACTAGGTAGTTAACTATGTACACTTAATTCTAATACATTTCTTAAAATTTAGACGAAGAGACATAGTTGAATTTTTCTTCTTGCCACCGCTCCTTTTATTTCATTTCCAACTTCAGCGACCTTTCCCCTTCTTTTCGCTTCAATTCATAATAAGAATATGTTGATGTTTGGGTTGGTGTGTTTTGCTCTGTGACTTTCCTTTGAAAATTCAACTCTCTAAATTTGCTTACAATAATATACACAGGAAATGCTCCGATTGCCCAATAACTCGATATTGAAATTGAGATGGAGCAGCATGCAACAATAGCATAAGGTCAGGAGGGGTGTTTCTTCTATCCAAACCATCATGTAAGGTATCTAGTTAGGGATATTAGTAATAGTATGGTATTTGTAAGGGTTAGTTAGAAGTTTGCGTAGTTGGTTATAAATAGAGGGAGGGGTGCATACTTTCCCTCAATTCCCCTATTCTCCTCCTATTTGTAACCAACTATATGCTAATTTCCTAGCTAACTACACTTATGCCCTTACTAATACAATACTAATACCCGGACCTTAAGTCTACCTTATCGTTAGTTGCTTGTGTGAAACATATGCCTACTCCCCATGTGCAATGGAGCCTCATATTCAACTTAATATTGATGGTAAGCCACCATTGCAATTAAACATTCGATTGCAGTAGAAACTCCCTTGAGTGAGTTCAAGTTGGGAGTTTGGGACATTACAAGTTTATGATGGTGAAGAAGTCTGACAAGCTCTTCTTTTTCGAAAAGCCATTGATAGGATTATGAATTTGATTGATTGATTGGATTAAAATGGATTGAATTTGAATTTGATTGATTTTTTTAGATTCATTTTTACTAAATGGATTGACTGGATTATGTGAGAGTTTGACTGAAACTGTGTTGATTGAAATAATGTAATTATGAAATTTGTTGGATGATGTTTCCATGTTATGTTCATGTTAGGTTTTAACTATTAGGCTAGTGTACCATGCATCTACGGCGT

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAA

Protein sequence

MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIGTQKVSLILISFIL
Homology
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BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 5.6e-94
Identity = 174/271 (64.21%), Postives = 209/271 (77.12%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSL---PMVATSTGENE------SDSDVSD-DEFSSIGVF 63
           VVHPAPGNA GTLVNAIL+HC +     +A ++ ++E       D DV D D+F++  V 
Sbjct: 159 VVHPAPGNANGTLVNAILHHCKISTFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVS 218

Query: 64  SRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPST 123
           S V EA +RPGIVHRLDKGTSGLLVVAKDEHSHA L+ QFK H+I RVYISLTCG P   
Sbjct: 219 SEVREALVRPGIVHRLDKGTSGLLVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPN 278

Query: 124 AGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRT 183
           +GRIE+PI RDPNNRIRM A  G+  + +A++AASRY V E+ A GGS+LVEWRLETGRT
Sbjct: 279 SGRIEVPIARDPNNRIRMIATPGS-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRT 338

Query: 184 HQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTL 243
           HQIRAHAKY+GIPLLGDE YGGTKSMA+SLLR R  S  H  L  ++S ++RPCLHA  L
Sbjct: 339 HQIRAHAKYLGIPLLGDETYGGTKSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALL 398

Query: 244 GFVHPHTGKNIRFSCPPPTDFTEILSQLREI 265
           GF HPH+GK + FSCPPP DFTE+L++L ++
Sbjct: 399 GFKHPHSGKILEFSCPPPDDFTEVLNELHQV 428

[Close]
BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-93
Identity = 172/265 (64.91%), Postives = 203/265 (76.60%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAP 63
           VVHPAPGN TGTLVN IL+HCSLP V  S  E + DSD    SDDE  +    S    A 
Sbjct: 167 VVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--AS 226

Query: 64  IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMP 123
           +RPGIVHRLDKGT+GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+P
Sbjct: 227 VRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIP 286

Query: 124 ICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHA 183
           I RD +NRIRM AI G      A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHA
Sbjct: 287 IGRDSSNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHA 346

Query: 184 KYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHT 243
           KYMG+PLLGDEVYGGTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP T
Sbjct: 347 KYMGVPLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCT 406

Query: 244 GKNIRFSCPPPTDFTEILSQLREIG 266
           G+ ++FSCPPP+D  EI+  LR  G
Sbjct: 407 GEIVKFSCPPPSDLAEIVGLLRRSG 429

[Close]
BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 7.4e-38
Identity = 101/258 (39.15%), Postives = 133/258 (51.55%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRP 63
           VVHPA GN   TLVNA+L HC+                    + S IG          RP
Sbjct: 101 VVHPAAGNYDNTLVNALLYHCA-------------------GKLSGIG-------GVARP 160

Query: 64  GIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICR 123
           GIVHR+DK TSGLLVVAK + +HA L+AQF  HSI R Y ++  G  PS  G ++ P+ R
Sbjct: 161 GIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSINRRYRAIVDG-HPSLQGHVDAPLAR 220

Query: 124 DPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYM 183
              NR +M  +    ++   K A + Y ++  L    +SL+E RLETGRTHQ+R H   +
Sbjct: 221 SSVNRQKMAIV----SDGRGKRAVTHYRMITPLK--NASLIECRLETGRTHQVRVHMSSI 280

Query: 184 GIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKN 243
           G  LLGD VYG +K    +LL++ A               +R  LHA  LGF+HP +GK 
Sbjct: 281 GHSLLGDPVYGRSKKAHHALLQSLA--------------FQRQALHAAHLGFIHPISGKQ 311

Query: 244 IRFSCPPPTDFTEILSQL 262
           + F    P D   +   L
Sbjct: 341 VDFDAEMPQDMQLLFKML 311

[Close]
BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 154.8 bits (390), Expect = 1.4e-36
Identity = 98/262 (37.40%), Postives = 132/262 (50.38%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRP 63
           VVHPAPG+ TGTLVN ++ HC+                    + S I          +RP
Sbjct: 99  VVHPAPGHLTGTLVNGLMAHCT--------------------DLSGI-------NGVMRP 158

Query: 64  GIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICR 123
           GIVHR+DK TSGLL+VAK++ +H  L  Q    ++ R Y ++  G+     G I+ PI R
Sbjct: 159 GIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVTRKYTAVVHGLISHDDGTIDAPIGR 218

Query: 124 DPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYM 183
           D  +R  MT     KN      A + ++V+E       +LVE +LETGRTHQIR H KY+
Sbjct: 219 DKKDRQSMTVTRDGKN------AVTHFHVLERFQD--FTLVECQLETGRTHQIRVHMKYI 278

Query: 184 GIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKN 243
           G PL GD  YG  K++           + +GQ            LHA  LGF HP TG+ 
Sbjct: 279 GFPLAGDPKYGPRKTI-----------DFNGQ-----------ALHAGVLGFDHPRTGEY 303

Query: 244 IRFSCPPPTDFTEILSQLREIG 266
           + F  P P D  E++  LR+ G
Sbjct: 339 VEFEAPLPEDMAELIENLRKNG 303

[Close]
BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-36
Identity = 101/267 (37.83%), Postives = 136/267 (50.94%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRP 63
           VVHPA GN TGTL NAIL+H               D+D  D             +  +RP
Sbjct: 96  VVHPAFGNWTGTLANAILHHLG------------KDADELD-------------KTEMRP 155

Query: 64  GIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICR 123
           GIVHRLDK TSGL+++AK+  +   L+ QF    +E+VY ++  GVP + +G I+  I R
Sbjct: 156 GIVHRLDKDTSGLIIIAKNPTALHKLARQFANRQVEKVYKAIVWGVPKAESGTIKTNIGR 215

Query: 124 DPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYM 183
              NR  M   +        K+A + Y V+E L  G  SL++  L TGRTHQIR H +++
Sbjct: 216 SHKNRKVMA--NFPYEGAEGKHAITDYQVVEDL--GYFSLMDVTLHTGRTHQIRVHLQHL 275

Query: 184 GIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKN 243
           G  +LGDE YGG         RT   S        L+  + R  LHA TL F  P TG+ 
Sbjct: 276 GHSILGDETYGGATP------RTLPFSKSEPFTRNLLELMSRQALHAQTLRFRQPTTGEP 327

Query: 244 IRFSCPPPTDFTEILSQLREIGTQKVS 271
           + FS P P D   +L ++R + T   S
Sbjct: 336 LSFSAPLPEDMELVLEKIRTVMTASQS 327

[Close]
BLAST of Cucsat.G6901 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 536 bits (1381), Expect = 2.13e-189
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR
Sbjct: 161 MVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 220

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC
Sbjct: 221 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 280

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 281 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 340

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 341 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 400

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQK 268
           NIRFSCPPPTDFTEILSQLREIGTQK
Sbjct: 401 NIRFSCPPPTDFTEILSQLREIGTQK 426

[Close]
BLAST of Cucsat.G6901 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 536 bits (1381), Expect = 3.49e-189
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR
Sbjct: 161 MVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 220

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC
Sbjct: 221 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 280

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 281 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 340

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 341 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 400

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQK 268
           NIRFSCPPPTDFTEILSQLREIGTQK
Sbjct: 401 NIRFSCPPPTDFTEILSQLREIGTQK 426

[Close]
BLAST of Cucsat.G6901 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 519 bits (1337), Expect = 9.47e-183
Identity = 256/268 (95.52%), Postives = 263/268 (98.13%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIR
Sbjct: 160 MVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIR 219

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PIC
Sbjct: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPIC 279

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTA  GTKNNPHAK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 280 RDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 339

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 340 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 399

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQKVS 270
           NIRFSCPPPTDF EILSQLREIGT+KV+
Sbjct: 400 NIRFSCPPPTDFAEILSQLREIGTKKVT 427

[Close]
BLAST of Cucsat.G6901 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 518 bits (1333), Expect = 4.13e-182
Identity = 255/267 (95.51%), Postives = 262/267 (98.13%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIR
Sbjct: 160 MVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIR 219

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PIC
Sbjct: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPIC 279

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTA  GTKNNPHAK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 280 RDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 339

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 340 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 399

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQKV 269
           NIRFSCPPPTDF EILSQLREIGT+K+
Sbjct: 400 NIRFSCPPPTDFAEILSQLREIGTKKL 426

[Close]
BLAST of Cucsat.G6901 vs. NCBI nr
Match: XP_038886062.1 (RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 503 bits (1295), Expect = 3.92e-176
Identity = 248/276 (89.86%), Postives = 261/276 (94.57%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMVATS GE  SD D+SDDEFSS+ +FSRVSEAPIR
Sbjct: 160 MVVHPAPGNATGTLVNAILNHCSLPMVATSKGELPSDMDISDDEFSSVEIFSRVSEAPIR 219

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFK HSIERVYISLTCGVPPSTAGRIE+PIC
Sbjct: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKLHSIERVYISLTCGVPPSTAGRIEVPIC 279

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTA+ GT NNPHAK+AASRY VMEILAQGGSSLVEWRLETGRTHQIRAHAK+
Sbjct: 280 RDPNNRIRMTAVSGTNNNPHAKHAASRYKVMEILAQGGSSLVEWRLETGRTHQIRAHAKH 339

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGD+VYGGTKSMAMSLLR+R SS+CH QLMQ +SSLERPCLHALTLGFVHPHTGK
Sbjct: 340 MGIPLLGDDVYGGTKSMAMSLLRSRTSSSCHSQLMQHISSLERPCLHALTLGFVHPHTGK 399

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQKVSLILISFIL 278
           NIRFSCPPPTDF EILSQLREIGT+KV+LIL SFIL
Sbjct: 400 NIRFSCPPPTDFAEILSQLREIGTKKVALILFSFIL 435

[Close]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 536 bits (1381), Expect = 1.03e-189
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR
Sbjct: 161 MVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 220

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC
Sbjct: 221 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 280

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 281 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 340

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 341 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 400

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQK 268
           NIRFSCPPPTDFTEILSQLREIGTQK
Sbjct: 401 NIRFSCPPPTDFTEILSQLREIGTQK 426

[Close]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 519 bits (1337), Expect = 4.59e-183
Identity = 256/268 (95.52%), Postives = 263/268 (98.13%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIR
Sbjct: 160 MVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIR 219

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PIC
Sbjct: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPIC 279

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTA  GTKNNPHAK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 280 RDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 339

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 340 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 399

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQKVS 270
           NIRFSCPPPTDF EILSQLREIGT+KV+
Sbjct: 400 NIRFSCPPPTDFAEILSQLREIGTKKVT 427

[Close]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 518 bits (1333), Expect = 2.00e-182
Identity = 255/267 (95.51%), Postives = 262/267 (98.13%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIR 62
            VVHPAPGNATGTLVNAILNHCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIR
Sbjct: 160 MVVHPAPGNATGTLVNAILNHCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIR 219

Query: 63  PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPIC 122
           PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PIC
Sbjct: 220 PGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPIC 279

Query: 123 RDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 182
           RDPNNRIRMTA  GTKNNPHAK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY
Sbjct: 280 RDPNNRIRMTATHGTKNNPHAKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKY 339

Query: 183 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 242
           MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK
Sbjct: 340 MGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGK 399

Query: 243 NIRFSCPPPTDFTEILSQLREIGTQKV 269
           NIRFSCPPPTDF EILSQLREIGT+K+
Sbjct: 400 NIRFSCPPPTDFAEILSQLREIGTKKL 426

[Close]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match: A0A6J1CM88 (RNA pseudouridine synthase 2, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 469 bits (1207), Expect = 4.88e-164
Identity = 234/269 (86.99%), Postives = 250/269 (92.94%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFS-RVSEAPI 62
           +VVHPAPGNATGTLVNAILNHCSLPMVATS GE  SD D+SDDEFSS  + S R SEAPI
Sbjct: 111 KVVHPAPGNATGTLVNAILNHCSLPMVATSNGEL-SDMDISDDEFSSNEILSSRASEAPI 170

Query: 63  RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPI 122
           RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI
Sbjct: 171 RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPI 230

Query: 123 CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAK 182
           CRDPNNRIRM+A+  T  NPHAKYAASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAK
Sbjct: 231 CRDPNNRIRMSAVLRTNKNPHAKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAK 290

Query: 183 YMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTG 242
           YMGIPLLGDEVYGGTKSMA+SLLRTR SS+CH QLMQLVS+LERPCLHALTLGF+HP+TG
Sbjct: 291 YMGIPLLGDEVYGGTKSMALSLLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTG 350

Query: 243 KNIRFSCPPPTDFTEILSQLREIGTQKVS 270
           +NIRFSCPPPTDF +I SQL EIGT+KV+
Sbjct: 351 ENIRFSCPPPTDFADIYSQLCEIGTKKVT 378

[Close]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 3.70e-163
Identity = 234/269 (86.99%), Postives = 249/269 (92.57%), Query Frame = 0 

Query: 3   QVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFS-RVSEAPI 62
            VVHPAPGNATGTLVNAILNHCSLPMVATS GE  SD D+SDDEFSS  + S R SEAPI
Sbjct: 159 MVVHPAPGNATGTLVNAILNHCSLPMVATSNGEL-SDMDISDDEFSSNEILSSRASEAPI 218

Query: 63  RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPI 122
           RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PI
Sbjct: 219 RPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPI 278

Query: 123 CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAK 182
           CRDPNNRIRM+A+  T  NPHAKYAASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAK
Sbjct: 279 CRDPNNRIRMSAVLRTNKNPHAKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAK 338

Query: 183 YMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTG 242
           YMGIPLLGDEVYGGTKSMA+SLLRTR SS+CH QLMQLVS+LERPCLHALTLGF+HP+TG
Sbjct: 339 YMGIPLLGDEVYGGTKSMALSLLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTG 398

Query: 243 KNIRFSCPPPTDFTEILSQLREIGTQKVS 270
           +NIRFSCPPPTDF +I SQL EIGT+KV+
Sbjct: 399 ENIRFSCPPPTDFADIYSQLCEIGTKKVT 426

[Close]
BLAST of Cucsat.G6901 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 344.4 bits (882), Expect = 8.8e-95
Identity = 172/265 (64.91%), Postives = 203/265 (76.60%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAP 63
           VVHPAPGN TGTLVN IL+HCSLP V  S  E + DSD    SDDE  +    S    A 
Sbjct: 167 VVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--AS 226

Query: 64  IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMP 123
           +RPGIVHRLDKGT+GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+P
Sbjct: 227 VRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIP 286

Query: 124 ICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHA 183
           I RD +NRIRM AI G      A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHA
Sbjct: 287 IGRDSSNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHA 346

Query: 184 KYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHT 243
           KYMG+PLLGDEVYGGTKSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP T
Sbjct: 347 KYMGVPLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCT 406

Query: 244 GKNIRFSCPPPTDFTEILSQLREIG 266
           G+ ++FSCPPP+D  EI+  LR  G
Sbjct: 407 GEIVKFSCPPPSDLAEIVGLLRRSG 429

[Close]
BLAST of Cucsat.G6901 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 178.7 bits (452), Expect = 6.4e-45
Identity = 96/149 (64.43%), Postives = 108/149 (72.48%), Query Frame = 0 

Query: 4   VVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAP 63
           VVHPAPGN TGTLVN IL+HCSLP V  S  E + DSD    SDDE  +    S    A 
Sbjct: 167 VVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--AS 226

Query: 64  IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMP 123
           +RPGIVHRLDKGT+GLLVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+P
Sbjct: 227 VRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIP 286

Query: 124 ICRDPNNRIRMTAIDGTKNNPHAKYAASR 150
           I RD +NRIRM AI G      A++AASR
Sbjct: 287 IGRDSSNRIRMAAIPGGVRGGRARHAASR 313

[Close]
BLAST of Cucsat.G6901 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-11
Identity = 50/155 (32.26%), Postives = 74/155 (47.74%), Query Frame = 0 

Query: 58  EAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIER 117
           E+P  P  VHRL +GTSG+L+ AK + +   L+A F +                   + +
Sbjct: 147 ESP-HPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSK 206

Query: 118 VYISLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQ 177
           +Y +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +
Sbjct: 207 IYRALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDRE 266

Query: 178 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 194
              SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 267 KNCSLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]
BLAST of Cucsat.G6901 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 4.6e-11
Identity = 59/222 (26.58%), Postives = 85/222 (38.29%), Query Frame = 0 

Query: 65  IVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPS------------ 124
           + +RLD+ TSG++++ K     A L   F +H I + YI+L  G  P+            
Sbjct: 115 LANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALCIGSSPNWRRVTVSSGHGR 174

Query: 125 ------------TAGRIEMP---ICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVME--- 184
                         GR+ +P     RD      + +++  KN        +   V E   
Sbjct: 175 SKHGAWRVYAALDVGRV-LPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAEGER 234

Query: 185 ILAQGGSS-----LVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRAS 244
            L+ GG       +V     +GRTHQIR H +Y+GIP+ GD  Y                
Sbjct: 235 ELSCGGDDDDVVVVVRAFPRSGRTHQIRLHCQYLGIPIRGDVKY---------------- 294

Query: 245 SNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPP 252
              HG       + E   LHA  L   HP TG +I    P P
Sbjct: 295 ---HGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLP 316

[Close]
BLAST of Cucsat.G6901 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 48/161 (29.81%), Postives = 76/161 (47.20%), Query Frame = 0 

Query: 58  EAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIER 117
           E+P  P  VHRL +GTSG+L+ AK + +   L+A F +                   + +
Sbjct: 177 ESP-HPVPVHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSK 236

Query: 118 VYISLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQ 177
           +Y +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +
Sbjct: 237 IYRALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDRE 296

Query: 178 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 200
              SLV+  +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 297 KNCSLVKVEIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

The following BLAST results are available for this feature:
BLAST of Cucsat.G6901 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q5Z8P25.6e-9464.21RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Q3ECD01.2e-9364.91RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
P505137.4e-3839.15Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... [more]
Q454801.4e-3637.40Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... [more]
O503102.4e-3637.83Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st... [more]
BLAST of Cucsat.G6901 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN62268.12.13e-18999.62hypothetical protein Csa_018737 [Cucumis sativus][more]
XP_031736623.13.49e-18999.62RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus][more]
XP_008445320.19.47e-18395.52PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo][more]
XP_008445318.14.13e-18295.51PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... [more]
XP_038886062.13.92e-17689.86RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida][more]
BLAST of Cucsat.G6901 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKD71.03e-18999.62Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1[more]
A0A1S3BD564.59e-18395.52Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BCE42.00e-18295.51Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A6J1CM884.88e-16486.99RNA pseudouridine synthase 2, chloroplastic isoform X2 OS=Momordica charantia OX... [more]
A0A6J1CMS33.70e-16386.99Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1[more]
BLAST of Cucsat.G6901 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G76050.28.8e-9564.91Pseudouridine synthase family protein [more]
AT1G76050.16.4e-4564.43Pseudouridine synthase family protein [more]
AT3G52260.22.1e-1132.26Pseudouridine synthase family protein [more]
AT1G56345.14.6e-1126.58Pseudouridine synthase family protein [more]
AT3G52260.31.1e-0929.81Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 77..139
e-value: 7.5E-5
score: 32.1
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 77..124
e-value: 6.3E-9
score: 35.4
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 77..156
e-value: 4.20157E-13
score: 62.267
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 134..408
e-value: 2.0E-67
score: 228.9
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 76..421
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 76..421
NoneNo IPR availablePROSITEPS50889S4coord: 77..150
score: 12.251314
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 76..164
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 63..132
e-value: 1.4E-15
score: 58.4
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 151..338
e-value: 2.8E-29
score: 102.2
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 152..393
e-value: 4.7871E-64
score: 202.182
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 77..420
e-value: 6.8E-75
score: 250.1
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 224..238
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 137..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G6901.T2Cucsat.G6901.T2mRNA
Cucsat.G6901.T4Cucsat.G6901.T4mRNA
Cucsat.G6901.T1Cucsat.G6901.T1mRNA
Cucsat.G6901.T3Cucsat.G6901.T3mRNA
Cucsat.G6901.T6Cucsat.G6901.T6mRNA
Cucsat.G6901.T5Cucsat.G6901.T5mRNA
Cucsat.G6901.T12Cucsat.G6901.T12mRNA
Cucsat.G6901.T11Cucsat.G6901.T11mRNA
Cucsat.G6901.T10Cucsat.G6901.T10mRNA
Cucsat.G6901.T7Cucsat.G6901.T7mRNA
Cucsat.G6901.T9Cucsat.G6901.T9mRNA
Cucsat.G6901.T8Cucsat.G6901.T8mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding