Cucsat.G6901.T8 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G6901.T8
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPseudouridine synthase
Locationctg1522: 100693 .. 105623 (+)
RNA-Seq ExpressionCucsat.G6901.T8
SyntenyCucsat.G6901.T8
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCCGATTAGTGAAAACCCAGAAACGAAACTATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTAGCGTCATTTTCCATAGAGAAGCTACATTTGCTAACCTTTTTTTTTTTCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTATGATTATCTAGTGGTCTGTAATCGGAGTGGTGAATTATGTTCAGCCTATATTTACTGTTTTTGTTCCATATTTGAGTTCTTAATTTGTGTGTTCATTTGCAACTTCTATATATTTTTTAAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTATTTGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCACGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCCTTTTTAAGCTTCCTTGTTACTGCTCATCTGAAGATTAAAGCAGACAATTCAAGTTTGGTGCTTCAAAAGTTTGCTACTCAAACACTTGTAATATCATGTTTAACTTCTAAGTTTTTTTCATTGGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTATGTACATAATTATTTTTCAGTGATAGAAAATATTTAAATATCACTGATTTTGGGCGTTCCTAACGCAAAGTTGAAACACCGAATGTTTCGCAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTAGTCTAAATGTCTTTCTCCTTAGGCTGTTTCAAATGACCAAAAAAAAAATTATATTACAATCTATCAATAGTCAGTTTAGAACATAAATTATATTCCCTAACACCACGCATATGTGCAAAGACATACATTTCTTTTGTTTGTAAAAGATTTCTCTATGATACTGCTACTGTTTTCCAGTCTTCATATGTGCATATGTAAAATAAAATTTCAATATAACGAGTCAGAGTTGCACCTCGAGCATACAAGCAGCCACATTTACCATTCCACATCCACTGCAATTTCAGTTCTTATCTTTTTTTAAAAAAAATTGAAATGCAGTTTTATTTTACTCGGTCTTAGGATTGAATATTTGTGAAGTAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAACAAATCTTTAAGAGCTCATTTAGTCCGTTCTTATTTCATTTACTATCCATTTTTTGAGAAATATGTGATTTTGACCTTTCTTCTTTATTTTATTTTATTTATTCTTTTCTAAAATAGTTTATAACTTTCATCTCTTATTTCTAATGTTTTGAAACTAAATGGAATTTATATTTGTATTATTTTCCTCTAATAATAATTGTATGCAAAATATCAAAACATTAATTTGACTATTCAATTCCAATTTTTCGTTTAAATTTCCACTTTTTGAAAAAGAAACGTTGTAGAGAAAAATAAATGATAACAACTGCTAACCACATTTTTGTTCTTTTTTAATAAAAATGGAGAAAAGCAAGAAATGGTATAGAAACATGTTTTCACTTTTTACAATAAAAGAGACTAATGAATCTTTACTTGTTCATTCCTTCCGTAAACAACTAATTCAAGATCACTGAATATTTTGTAGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAAGTTGAGCCAAGCTTTTTGCTTGGTAATCGGTTATTTTACAGATTTAATATCCATCCATCATCATGAAGAACAATATAAAAAATGTCCCGAGGGATACAATTGACACAACCAACCTCATTCGTAGCAACCTAGGTAGTTAATACGTGTCACCTTTATGCCTATTTTCTGGGCGCCCCCTTGCCATGTTATAGCTAATACCGGGTCTTCTCTATTTACTAGCTATTTTTCACCTGGTTCTTGTGTTTGGAGTGACTATTTGTTGGTTTAGTTTCCTTTTGTACTTCATCGCTAACTTATTCTACTAAACTTAACACAAAAATAAGTTGTTTAATGATGGGTGAAAATTTAGCAATACATAAAATATATTTTCTTATTATTTAAACGATGGTTTTATACATGAAAACATTGGAGACATGATAGATATAACATAATAACTAAATAGCAGACAAACAAGCATAGAAGCTCTCCTTTTACTTGCTTCTCTTATAAATAGTAACGAATAGGGACGTTTGTTCTATCTATACAAATGACATAATGGGGATAAATTCCCTGAACAATTTTGAACTCTATATCATGTGGAAATTTCGCATCATATCTTGTGGATTAATCTTATGCCGGTCTGATGGTAGATAGGGCAAATTTGAAATAATTTTGAGCTTCACAAAATATATTAGATGTTGAACAATGGTTACTACCATAGAATCTAGGTGAGATGTGGTTTTTACGGTGCCCCAGTTAACCTATAATAGAGTGTTGTCATAAAAGTGGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCGTCAAATGACTCGAAAATGAGATATCAAGAAGGTACATTAGGACATACAGCCATTTAAGTTGTGATGTCTGAAGCACAAATTTAAGTTCTGGGTAACTTACATATCTTGTTTATTAGATTCATTTAATTGTTGAAATTTAAAGTATTTTGTCACACATTCCCAGACGTTGAATTCCACGTATTGTTAATCTCTGCCATTAACTTCGATTATTGAAACCATTTAGAATCAATATTGTATATACGGGTAGTTACTAGGTAGTTAACTATGTACACTTAATTCTAATACATTTCTTAAAATTTAGACGAAGAGACATAGTTGAATTTTTCTTCTTGCCACCGCTCCTTTTATTTCATTTCCAACTTCAGCGACCTTTCCCCTTCTTTTCGCTTCAATTCATAATAAGAATATGTTGATGTTTGGGTTGGTGTGTTTTGCTCTGTGACTTTCCTTTGAAAATTCAACTCTCTAAATTTGCTTACAATAATATACACAGGAAATGCTCCGATTGCCCAATAACTCGATATTGAAATTGAGATGGAGCAGCATGCAACAATAGCATAAGGTCAGGAGGGGTGTTTCTTCTATCCAAACCATCATGTAAGGTATCTAGTTAGGGATATTAGTAATAGTATGGTATTTGTAAGGGTTAGTTAGAAGTTTGCGTAGTTGGTTATAAATAGAGGGAGGGGTGCATACTTTCCCTCAATTCCCCTATTCTCCTCCTATTTGTAACCAACTATATGCTAATTTCCTAGCTAACTACACTTATGCCCTTACTAATACAATACTAATACCCGGACCTTAAGTCTACCTTATCGTTAGTTGCTTGTGTGAAACATATGCCTACTCCCCATGTGCAATGGAGCCTCATATTCAACTTAATATTGATGGTAAGCCACCATTGCAATTAAACATTCGATTGCAGTAGAAACTCCCTTGAGTGAGTTCAAGTTGGGAGTTTGGGACATTACAAGTTTATGATGGTGAAGAAGTCTGACAAGCTCTTCTTTTTCGAAAAGCCATTGATAGGATTATGAATTTGATTGATTGATTGGATTAAAATGGATTGAATTTGAATTTGATTGATTTTTTTAGATTCATTTTTACTAAATGGATTGACTGGATTATGTGAGAGTTTGACTGAAACTGTGTTGATTGAAATAATGTAATTATGAAATTTGTTGGATGATGTTTCCATGTTATGTTCATGTTAGGTTTTAACTATTAGGCTAGTGTACCATGCATCTACGG

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTATTTGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCACGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAA

Protein sequence

MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVMIQKSLILLLSILFEVICGCKISILVDKIKRSPLPRLKFLVRLKFARICVSGFLHDSGCSSSTWKCDRNTSKCHS
Homology
[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.9e-41
Identity = 97/166 (58.43%), Postives = 116/166 (69.88%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVM 164
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMV+
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVV 168

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-34
Identity = 90/182 (49.45%), Postives = 115/182 (63.19%), Query Frame = 0 

Query: 7   ARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRC-SSDYDEYSEAQSERIANYAGTR 66
           A T  ASP  I        RR      R       RC ++D +  + A S R   + GTR
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRR-GGHGGTR 61

Query: 67  LEETVDVNPGKVRLDSWISCRI--NGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDV 126
           LEE V    G+ R+D+WIS R+   G+SRAR+Q+SIRAGLV VNGR + KVSH VK GD+
Sbjct: 62  LEEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDI 121

Query: 127 INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVMIQKSLILLLSILFEVICGCK 186
           ++CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMV+         +++  ++  CK
Sbjct: 122 VSCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCK 181

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match: P74346 (Uncharacterized RNA pseudouridine synthase slr1629 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1629 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-10
Identity = 36/87 (41.38%), Postives = 55/87 (63.22%), Query Frame = 0 

Query: 77  VRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRA 136
           VRLD W++ ++  +SR+R Q  I +G V  NG V    +  +K GD +   I EL PL  
Sbjct: 32  VRLDKWLAEQLPELSRSRCQKLIESGQVQRNGLVCQDKNLILKTGDRLVVNIPELIPLDV 91

Query: 137 EPEDIDLDIVYEDEHVLVVNKPAHMVM 164
             ++I LDI+YEDE ++++NKPA +V+
Sbjct: 92  VAQNIPLDILYEDEQLIIINKPAGLVV 118

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match: P65834 (Ribosomal large subunit pseudouridine synthase D OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=rluD PE=3 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 38/99 (38.38%), Postives = 54/99 (54.55%), Query Frame = 0 

Query: 65  RLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVI 124
           +L  TV  N    RLD  ++      SR+R++  I    V VNG+V DK    V  G+ +
Sbjct: 6   QLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQRVLVNGKVCDKPKEKVLGGEQV 65

Query: 125 NCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVM 164
                  +  R EP+DI LDIVYEDE ++V+NKP  +V+
Sbjct: 66  AINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDLVV 104

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match: P65835 (Ribosomal large subunit pseudouridine synthase D OS=Shigella flexneri OX=623 GN=rluD PE=3 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 38/99 (38.38%), Postives = 54/99 (54.55%), Query Frame = 0 

Query: 65  RLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVI 124
           +L  TV  N    RLD  ++      SR+R++  I    V VNG+V DK    V  G+ +
Sbjct: 6   QLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQRVLVNGKVCDKPKEKVLGGEQV 65

Query: 125 NCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVM 164
                  +  R EP+DI LDIVYEDE ++V+NKP  +V+
Sbjct: 66  AINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDLVV 104

[Close]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 323 bits (827), Expect = 5.43e-106
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162

[Close]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 323 bits (827), Expect = 8.35e-106
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162

[Close]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match: KAA0064849.1 (RNA pseudouridine synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 299 bits (766), Expect = 3.40e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 299 bits (766), Expect = 8.23e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 299 bits (766), Expect = 8.74e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 323 bits (827), Expect = 2.63e-106
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match: A0A5A7VHA3 (Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001610 PE=3 SV=1)

HSP 1 Score: 299 bits (766), Expect = 1.64e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 299 bits (766), Expect = 3.98e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 299 bits (766), Expect = 4.23e-97
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 161

[Close]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match: A0A6J1CNK7 (RNA pseudouridine synthase 2, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 262 bits (669), Expect = 1.74e-83
Identity = 136/162 (83.95%), Postives = 144/162 (88.89%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCS R IQA+PS I  FFP NR RI SNV RI++N STRCS DYDE  EA SERI N
Sbjct: 1   MISVCSVRAIQAAPS-IQLFFPRNRGRITSNVHRIATNHSTRCS-DYDECGEAHSERITN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEE VDVN GK+RLDSWISCRINGISRARVQSSIRAGLV+VNGRVIDKVSHN+KA
Sbjct: 61  YAGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMV 162
           GD+INCTISELQPLRA+PEDIDLDIVYEDEHVLVVNKPAHMV
Sbjct: 121 GDMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMV 160

[Close]
BLAST of Cucsat.G6901.T8 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 168.7 bits (426), Expect = 5.6e-42
Identity = 97/166 (58.43%), Postives = 116/166 (69.88%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVM 164
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMV+
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVV 168

[Close]
BLAST of Cucsat.G6901.T8 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 168.7 bits (426), Expect = 5.6e-42
Identity = 97/166 (58.43%), Postives = 116/166 (69.88%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVM 164
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMV+
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVV 168

The following BLAST results are available for this feature:
BLAST of Cucsat.G6901.T8 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3ECD07.9e-4158.43RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5Z8P21.4e-3449.45RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
P743461.5e-1041.38Uncharacterized RNA pseudouridine synthase slr1629 OS=Synechocystis sp. (strain ... [more]
P658342.1e-0938.38Ribosomal large subunit pseudouridine synthase D OS=Escherichia coli O6:H1 (stra... [more]
P658352.1e-0938.38Ribosomal large subunit pseudouridine synthase D OS=Shigella flexneri OX=623 GN=... [more]
BLAST of Cucsat.G6901.T8 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN62268.15.43e-106100.00hypothetical protein Csa_018737 [Cucumis sativus][more]
XP_031736623.18.35e-106100.00RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus][more]
KAA0064849.13.40e-9794.44RNA pseudouridine synthase 2 [Cucumis melo var. makuwa][more]
XP_008445320.18.23e-9794.44PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo][more]
XP_008445318.18.74e-9794.44PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... [more]
BLAST of Cucsat.G6901.T8 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKD72.63e-106100.00Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1[more]
A0A5A7VHA31.64e-9794.44Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3BD563.98e-9794.44Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BCE44.23e-9794.44Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A6J1CNK71.74e-8383.95RNA pseudouridine synthase 2, chloroplastic isoform X3 OS=Momordica charantia OX... [more]
BLAST of Cucsat.G6901.T8 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G76050.15.6e-4258.43Pseudouridine synthase family protein [more]
AT1G76050.25.6e-4258.43Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 77..139
e-value: 7.5E-5
score: 32.1
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 77..124
e-value: 2.6E-9
score: 36.7
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 77..156
e-value: 3.47284E-12
score: 58.0298
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 134..205
e-value: 1.1E-5
score: 27.1
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 76..163
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 76..163
NoneNo IPR availablePROSITEPS50889S4coord: 77..150
score: 12.251314
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 76..163
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 60..132
e-value: 4.9E-16
score: 59.9

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G6901Cucsat.G6901gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T8.E1Cucsat.G6901.T8.E1exon
Cucsat.G6901.T8.E2Cucsat.G6901.T8.E2exon
Cucsat.G6901.T8.E3Cucsat.G6901.T8.E3exon
Cucsat.G6901.T8.E4Cucsat.G6901.T8.E4exon
Cucsat.G6901.T8.E5Cucsat.G6901.T8.E5exon
Cucsat.G6901.T8.E6Cucsat.G6901.T8.E6exon
Cucsat.G6901.T8.E7Cucsat.G6901.T8.E7exon
Cucsat.G6901.T8.E8Cucsat.G6901.T8.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T8.C1Cucsat.G6901.T8.C1CDS
Cucsat.G6901.T8.C2Cucsat.G6901.T8.C2CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T8Cucsat.G6901.T8-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
molecular_function GO:0003723 RNA binding
molecular_function GO:0009982 pseudouridine synthase activity