Cucsat.G6901.T6 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G6901.T6
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pseudouridine synthase
Location	ctg1522: 100655 .. 105515 (+)
RNA-Seq Expression	Cucsat.G6901.T6
Synteny	Cucsat.G6901.T6

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAAAGAAAAACCCAATTTTCCCGGTAAGAAAACAGACAGCCGATTAGTGAAAACCCAGAAACGAAACTATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTAGCGTCATTTTCCATAGAGAAGCTACATTTGCTAACCTTTTTTTTTTTCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTATGATTATCTAGTGGTCTGTAATCGGAGTGGTGAATTATGTTCAGCCTATATTTACTGTTTTTGTTCCATATTTGAGTTCTTAATTTGTGTGTTCATTTGCAACTTCTATATATTTTTTAAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTATTTGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCACGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCCTTTTTAAGCTTCCTTGTTACTGCTCATCTGAAGATTAAAGCAGACAATTCAAGTTTGGTGCTTCAAAAGTTTGCTACTCAAACACTTGTAATATCATGTTTAACTTCTAAGTTTTTTTCATTGGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTATGTACATAATTATTTTTCAGTGATAGAAAATATTTAAATATCACTGATTTTGGGCGTTCCTAACGCAAAGTTGAAACACCGAATGTTTCGCAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTAGTCTAAATGTCTTTCTCCTTAGGCTGTTTCAAATGACCAAAAAAAAAATTATATTACAATCTATCAATAGTCAGTTTAGAACATAAATTATATTCCCTAACACCACGCATATGTGCAAAGACATACATTTCTTTTGTTTGTAAAAGATTTCTCTATGATACTGCTACTGTTTTCCAGTCTTCATATGTGCATATGTAAAATAAAATTTCAATATAACGAGTCAGAGTTGCACCTCGAGCATACAAGCAGCCACATTTACCATTCCACATCCACTGCAATTTCAGTTCTTATCTTTTTTTAAAAAAAATTGAAATGCAGTTTTATTTTACTCGGTCTTAGGATTGAATATTTGTGAAGTAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAACAAATCTTTAAGAGCTCATTTAGTCCGTTCTTATTTCATTTACTATCCATTTTTTGAGAAATATGTGATTTTGACCTTTCTTCTTTATTTTATTTTATTTATTCTTTTCTAAAATAGTTTATAACTTTCATCTCTTATTTCTAATGTTTTGAAACTAAATGGAATTTATATTTGTATTATTTTCCTCTAATAATAATTGTATGCAAAATATCAAAACATTAATTTGACTATTCAATTCCAATTTTTCGTTTAAATTTCCACTTTTTGAAAAAGAAACGTTGTAGAGAAAAATAAATGATAACAACTGCTAACCACATTTTTGTTCTTTTTTAATAAAAATGGAGAAAAGCAAGAAATGGTATAGAAACATGTTTTCACTTTTTACAATAAAAGAGACTAATGAATCTTTACTTGTTCATTCCTTCCGTAAACAACTAATTCAAGATCACTGAATATTTTGTAGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAAGTTGAGCCAAGCTTTTTGCTTGGTAATCGGTTATTTTACAGATTTAATATCCATCCATCATCATGAAGAACAATATAAAAAATGTCCCGAGGGATACAATTGACACAACCAACCTCATTCGTAGCAACCTAGGTAGTTAATACGTGTCACCTTTATGCCTATTTTCTGGGCGCCCCCTTGCCATGTTATAGCTAATACCGGGTCTTCTCTATTTACTAGCTATTTTTCACCTGGTTCTTGTGTTTGGAGTGACTATTTGTTGGTTTAGTTTCCTTTTGTACTTCATCGCTAACTTATTCTACTAAACTTAACACAAAAATAAGTTGTTTAATGATGGGTGAAAATTTAGCAATACATAAAATATATTTTCTTATTATTTAAACGATGGTTTTATACATGAAAACATTGGAGACATGATAGATATAACATAATAACTAAATAGCAGACAAACAAGCATAGAAGCTCTCCTTTTACTTGCTTCTCTTATAAATAGTAACGAATAGGGACGTTTGTTCTATCTATACAAATGACATAATGGGGATAAATTCCCTGAACAATTTTGAACTCTATATCATGTGGAAATTTCGCATCATATCTTGTGGATTAATCTTATGCCGGTCTGATGGTAGATAGGGCAAATTTGAAATAATTTTGAGCTTCACAAAATATATTAGATGTTGAACAATGGTTACTACCATAGAATCTAGGTGAGATGTGGTTTTTACGGTGCCCCAGTTAACCTATAATAGAGTGTTGTCATAAAAGTGGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCGTCAAATGACTCGAAAATGAGATATCAAGAAGGTACATTAGGACATACAGCCATTTAAGTTGTGATGTCTGAAGCACAAATTTAAGTTCTGGGTAACTTACATATCTTGTTTATTAGATTCATTTAATTGTTGAAATTTAAAGTATTTTGTCACACATTCCCAGACGTTGAATTCCACGTATTGTTAATCTCTGCCATTAACTTCGATTATTGAAACCATTTAGAATCAATATTGTATATACGGGTAGTTACTAGGTAGTTAACTATGTACACTTAATTCTAATACATTTCTTAAAATTTAGACGAAGAGACATAGTTGAATTTTTCTTCTTGCCACCGCTCCTTTTATTTCATTTCCAACTTCAGCGACCTTTCCCCTTCTTTTCGCTTCAATTCATAATAAGAATATGTTGATGTTTGGGTTGGTGTGTTTTGCTCTGTGACTTTCCTTTGAAAATTCAACTCTCTAAATTTGCTTACAATAATATACACAGGAAATGCTCCGATTGCCCAATAACTCGATATTGAAATTGAGATGGAGCAGCATGCAACAATAGCATAAGGTCAGGAGGGGTGTTTCTTCTATCCAAACCATCATGTAAGGTATCTAGTTAGGGATATTAGTAATAGTATGGTATTTGTAAGGGTTAGTTAGAAGTTTGCGTAGTTGGTTATAAATAGAGGGAGGGGTGCATACTTTCCCTCAATTCCCCTATTCTCCTCCTATTTGTAACCAACTATATGCTAATTTCCTAGCTAACTACACTTATGCCCTTACTAATACAATACTAATACCCGGACCTTAAGTCTACCTTATCGTTAGTTGCTTGTGTGAAACATATGCCTACTCCCCATGTGCAATGGAGCCTCATATTCAACTTAATATTGATGGTAAGCCACCATTGCAATTAAACATTCGATTGCAGTAGAAACTCCCTTGAGTGAGTTCAAGTTGGGAGTTTGGGACATTACAAGTTTATGATGGTGAAGAAGTCTGACAAGCTCTTCTTTTTCGAAAAGCCATTGATAGGATTATGAATTTGATTGATTGATTGGATTAAAATGGATTGAATTTGAATTTGATTGATTTTTTTAGATTCATTTTTACTAAATGGATTGACTGGATTATGTGAGAGTTTGACTG

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAA

Protein sequence

MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIGTQKVSLILISFIL

Homology

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 268/429 (62.47%), Postives = 317/429 (73.89%), Query Frame = 0


Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 362
                 A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYGGT
Sbjct: 311 GVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGT 370

Query: 363 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTE 422
           KSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D  E
Sbjct: 371 KSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDLAE 429

Query: 423 ILSQLREIG 424
           I+  LR  G
Sbjct: 431 IVGLLRRSG 429

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.2e-137
Identity = 259/429 (60.37%), Postives = 313/429 (72.96%), Query Frame = 0


Query: 7   ARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRC-SSDYDEYSEAQSERIANYAGTR 66
           A T  ASP  I        RR      R       RC ++D +  + A S R   + GTR
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRR-GGHGGTR 61

Query: 67  LEETVDVNPGKVRLDSWISCRI--NGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDV 126
           LEE V    G+ R+D+WIS R+   G+SRAR+Q+SIRAGLV VNGR + KVSH VK GD+
Sbjct: 62  LEEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDI 121

Query: 127 INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCS 186
           ++CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL+HC 
Sbjct: 122 VSCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCK 181

Query: 187 L---PMVATSTGENE------SDSDVSD-DEFSSIGVFSRVSEAPIRPGIVHRLDKGTSG 246
           +     +A ++ ++E       D DV D D+F++  V S V EA +RPGIVHRLDKGTSG
Sbjct: 182 ISTFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDKGTSG 241

Query: 247 LLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAID 306
           LLVVAKDEHSHA L+ QFK H+I RVYISLTCG P   +GRIE+PI RDPNNRIRM A  
Sbjct: 242 LLVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRMIATP 301

Query: 307 GTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGG 366
           G+  + +A++AASRY V E+ A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDE YGG
Sbjct: 302 GS-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDETYGG 361

Query: 367 TKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFT 423
           TKSMA+SLLR R  S  H  L  ++S ++RPCLHA  LGF HPH+GK + FSCPPP DFT
Sbjct: 362 TKSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKHPHSGKILEFSCPPPDDFT 421

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.8e-54
Identity = 136/343 (39.65%), Postives = 185/343 (53.94%), Query Frame = 0


Query: 78  RLDSWISCRINGISRARVQSSIRAGLVS-VNGRVIDKVSHNVKAGDVINCTISELQPLRA 137
           RLD  ++  I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 138 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENES 197
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+             
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA------------- 135

Query: 198 DSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 257
                  + S IG          RPGIVHR+DK TSGLLVVAK + +HA L+AQF  HSI
Sbjct: 136 ------GKLSGIG-------GVARPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSI 195

Query: 258 ERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQ 317
            R Y ++  G  PS  G ++ P+ R   NR +M  +    ++   K A + Y ++  L  
Sbjct: 196 NRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIV----SDGRGKRAVTHYRMITPLK- 255

Query: 318 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQ 377
             +SL+E RLETGRTHQ+R H   +G  LLGD VYG +K    +LL++ A          
Sbjct: 256 -NASLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSKKAHHALLQSLA---------- 311

Query: 378 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420
                +R  LHA  LGF+HP +GK + F    P D   +   L
Sbjct: 316 ----FQRQALHAAHLGFIHPISGKQVDFDAEMPQDMQLLFKML 311

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 213.0 bits (541), Expect = 6.7e-54
Identity = 132/346 (38.15%), Postives = 183/346 (52.89%), Query Frame = 0


Query: 78  RLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAE 137
           R+D +++   N  SR +VQ  ++ G V VNG  + K ++ ++ GD +  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 138 PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESD 197
            E +DLDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 198 SDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIE 257
                 + S I          +RPGIVHR+DK TSGLL+VAK++ +H  L  Q    ++ 
Sbjct: 136 ------DLSGI-------NGVMRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVT 195

Query: 258 RVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQG 317
           R Y ++  G+     G I+ PI RD  +R  MT     KN      A + ++V+E     
Sbjct: 196 RKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMTVTRDGKN------AVTHFHVLERFQD- 255

Query: 318 GSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQL 377
             +LVE +LETGRTHQIR H KY+G PL GD  YG  K++           + +GQ    
Sbjct: 256 -FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI-----------DFNGQ---- 303

Query: 378 VSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIG 424
                   LHA  LGF HP TG+ + F  P P D  E++  LR+ G
Sbjct: 316 -------ALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRKNG 303

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.4e-51
Identity = 133/362 (36.74%), Postives = 196/362 (54.14%), Query Frame = 0


Query: 69  TVDVNPGK--VRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINC 128
           T+DV+  +  +R+D +++ ++  ++R +VQ++I  G V VNG+ + K ++ VK+ D I+ 
Sbjct: 2   TLDVSKVQTPMRIDRYLTQQVENMTRNKVQAAIEEGRVLVNGKPV-KSNYRVKSCDHIHL 61

Query: 129 TISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPM 188
           T          PEDI + I+YED+ ++V++K A MVVHPA GN TGTL NAIL+H     
Sbjct: 62  TFLRPPAPELAPEDIPITILYEDDDLMVIDKEAGMVVHPAFGNWTGTLANAILHHLG--- 121

Query: 189 VATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAH 248
                     D+D  D             +  +RPGIVHRLDK TSGL+++AK+  +   
Sbjct: 122 ---------KDADELD-------------KTEMRPGIVHRLDKDTSGLIIIAKNPTALHK 181

Query: 249 LSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAAS 308
           L+ QF    +E+VY ++  GVP + +G I+  I R   NR  M   +        K+A +
Sbjct: 182 LARQFANRQVEKVYKAIVWGVPKAESGTIKTNIGRSHKNRKVMA--NFPYEGAEGKHAIT 241

Query: 309 RYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRA 368
            Y V+E L  G  SL++  L TGRTHQIR H +++G  +LGDE YGG         RT  
Sbjct: 242 DYQVVEDL--GYFSLMDVTLHTGRTHQIRVHLQHLGHSILGDETYGGATP------RTLP 301

Query: 369 SSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIGTQK 428
            S        L+  + R  LHA TL F  P TG+ + FS P P D   +L ++R + T  
Sbjct: 302 FSKSEPFTRNLLELMSRQALHAQTLRFRQPTTGEPLSFSAPLPEDMELVLEKIRTVMTAS 327

[Close]

BLAST of Cucsat.G6901.T6 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 854 bits (2207), Expect = 1.89e-312
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQK 426
           EIGTQK
Sbjct: 421 EIGTQK 426

[Close]

BLAST of Cucsat.G6901.T6 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 854 bits (2207), Expect = 3.21e-312
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQK 426
           EIGTQK
Sbjct: 421 EIGTQK 426

[Close]

BLAST of Cucsat.G6901.T6 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 814 bits (2102), Expect = 1.69e-296
Identity = 408/428 (95.33%), Postives = 418/428 (97.66%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKVS 428
           EIGT+KV+
Sbjct: 421 EIGTKKVT 427

[Close]

BLAST of Cucsat.G6901.T6 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 812 bits (2098), Expect = 7.44e-296
Identity = 407/427 (95.32%), Postives = 417/427 (97.66%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKV 427
           EIGT+K+
Sbjct: 421 EIGTKKL 426

[Close]

BLAST of Cucsat.G6901.T6 vs. NCBI nr
Match: XP_038886062.1 (RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 776 bits (2004), Expect = 2.55e-281
Identity = 391/436 (89.68%), Postives = 408/436 (93.58%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS I  FFP NRRRI  +VLR S N STR SSDYDEY EAQSER AN
Sbjct: 1   MISVCSARTIQASPSSIQLFFPRNRRRITPDVLRFSVNLSTR-SSDYDEYGEAQSERRAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           +AG RLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGR+IDKVSHNVKA
Sbjct: 61  FAGIRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRIIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRAEPEDIDLDIVYED HVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDMINCTISELQPLRAEPEDIDLDIVYEDAHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATS GE  SD D+SDDEFSS+ +FSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSKGELPSDMDISDDEFSSVEIFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFK HSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA+ GT NNPH
Sbjct: 241 EHSHAHLSAQFKLHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMTAVSGTNNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRY VMEILAQGGSSLVEWRLETGRTHQIRAHAK+MGIPLLGD+VYGGTKSMAMS
Sbjct: 301 AKHAASRYKVMEILAQGGSSLVEWRLETGRTHQIRAHAKHMGIPLLGDDVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLR+R SS+CH QLMQ +SSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRSRTSSSCHSQLMQHISSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKVSLILISFIL 436
           EIGT+KV+LIL SFIL
Sbjct: 421 EIGTKKVALILFSFIL 435

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 9.13e-313
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQK 426
           EIGTQK
Sbjct: 421 EIGTQK 426

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 814 bits (2102), Expect = 8.20e-297
Identity = 408/428 (95.33%), Postives = 418/428 (97.66%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKVS 428
           EIGT+KV+
Sbjct: 421 EIGTKKVT 427

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 3.60e-296
Identity = 407/427 (95.32%), Postives = 417/427 (97.66%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKV 427
           EIGT+K+
Sbjct: 421 EIGTKKL 426

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL
Match: A0A5A7VHA3 (Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001610 PE=3 SV=1)

HSP 1 Score: 742 bits (1916), Expect = 6.02e-269
Identity = 375/392 (95.66%), Postives = 383/392 (97.70%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

[Close]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 726 bits (1874), Expect = 4.29e-262
Identity = 369/429 (86.01%), Postives = 392/429 (91.38%), Query Frame = 0


Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCS R IQA+PS I  FFP NR RI SNV RI++N STRCS DYDE  EA SERI N
Sbjct: 1   MISVCSVRAIQAAPS-IQLFFPRNRGRITSNVHRIATNHSTRCS-DYDECGEAHSERITN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEE VDVN GK+RLDSWISCRINGISRARVQSSIRAGLV+VNGRVIDKVSHN+KA
Sbjct: 61  YAGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRA+PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFS-RVSEAPIRPGIVHRLDKGTSGLLVVAK 240
           HCSLPMVATS GE  SD D+SDDEFSS  + S R SEAPIRPGIVHRLDKGTSGLLVVAK
Sbjct: 181 HCSLPMVATSNGEL-SDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNP 300
           DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRM+A+  T  NP
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNP 300

Query: 301 HAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           HAKYAASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+
Sbjct: 301 HAKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAL 360

Query: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420
           SLLRTR SS+CH QLMQLVS+LERPCLHALTLGF+HP+TG+NIRFSCPPPTDF +I SQL
Sbjct: 361 SLLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQL 420

Query: 421 REIGTQKVS 428
            EIGT+KV+
Sbjct: 421 CEIGTKKVT 426

[Close]

BLAST of Cucsat.G6901.T6 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 268/429 (62.47%), Postives = 317/429 (73.89%), Query Frame = 0


Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 362
                 A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYGGT
Sbjct: 311 GVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGT 370

Query: 363 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTE 422
           KSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D  E
Sbjct: 371 KSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDLAE 429

Query: 423 ILSQLREIG 424
           I+  LR  G
Sbjct: 431 IVGLLRRSG 429

[Close]

BLAST of Cucsat.G6901.T6 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 192/313 (61.34%), Postives = 222/313 (70.93%), Query Frame = 0


Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASR 308
                 A++AASR
Sbjct: 311 GVRGGRARHAASR 313

[Close]

BLAST of Cucsat.G6901.T6 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-14
Identity = 70/272 (25.74%), Postives = 119/272 (43.75%), Query Frame = 0


Query: 99  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 158
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 159 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAP 218
           + + V P                  L  +    G+N+S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 219 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 278
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 279 SLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGS 338
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 295

Query: 339 SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 352
           SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]

BLAST of Cucsat.G6901.T6 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.8e-12
Identity = 67/266 (25.19%), Postives = 114/266 (42.86%), Query Frame = 0


Query: 99  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 158
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 159 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAP 218
           + + V P                  L  +    G+N+S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 219 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 278
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 279 SLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGS 338
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 289

Query: 339 SLVEWRLETGRTHQIRAHAKYMGIPL 346
           SLV+  +++GR HQIR H  YMG PL
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPL 289

[Close]

BLAST of Cucsat.G6901.T6 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.8e-12
Identity = 66/273 (24.18%), Postives = 118/273 (43.22%), Query Frame = 0


Query: 104 VSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVV 163
           + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP+ + V
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQV 150

Query: 164 HPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGI 223
            P                  L  +    G+N+S             + SR S  P+    
Sbjct: 151 LPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHPVP--- 210

Query: 224 VHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYISLTCG 283
           VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y +L  G
Sbjct: 211 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 270

Query: 284 VPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEW 343
           +       I+ PI   R P       A      +P  K A S+  V+E   +   SLV+ 
Sbjct: 271 IVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKV 330

Query: 344 RLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 358
            +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 331 EIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

The following BLAST results are available for this feature:

BLAST of Cucsat.G6901.T6 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3ECD0	1.7e-142	62.47	RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q5Z8P2	2.2e-137	60.37	RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3...	[more]
P50513	1.8e-54	39.65	Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob...	[more]
Q45480	6.7e-54	38.15	Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168...	[more]
O50310	2.4e-51	36.74	Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st...	[more]

BLAST of Cucsat.G6901.T6 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62268.1	1.89e-312	100.00	hypothetical protein Csa_018737 [Cucumis sativus]	[more]
XP_031736623.1	3.21e-312	100.00	RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus]	[more]
XP_008445320.1	1.69e-296	95.33	PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo]	[more]
XP_008445318.1	7.44e-296	95.32	PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]...	[more]
XP_038886062.1	2.55e-281	89.68	RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida]	[more]

BLAST of Cucsat.G6901.T6 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKD7	9.13e-313	100.00	Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1	[more]
A0A1S3BD56	8.20e-297	95.33	Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1	[more]
A0A1S3BCE4	3.60e-296	95.32	Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1	[more]
A0A5A7VHA3	6.02e-269	95.66	Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8...	[more]
A0A6J1CMS3	4.29e-262	86.01	Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1	[more]

BLAST of Cucsat.G6901.T6 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76050.2	1.2e-143	62.47	Pseudouridine synthase family protein	[more]
AT1G76050.1	1.2e-93	61.34	Pseudouridine synthase family protein	[more]
AT3G52260.2	1.4e-14	25.74	Pseudouridine synthase family protein	[more]
AT3G52260.1	3.8e-12	25.19	Pseudouridine synthase family protein	[more]
AT3G52260.3	3.8e-12	24.18	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002942	RNA-binding S4 domain	SMART	SM00363	s4_6	coord: 77..139 e-value: 7.5E-5 score: 32.1
IPR002942	RNA-binding S4 domain	PFAM	PF01479	S4	coord: 77..124 e-value: 6.4E-9 score: 35.4
IPR002942	RNA-binding S4 domain	CDD	cd00165	S4	coord: 77..156 e-value: 6.0375E-13 score: 61.8818
IPR006225	Pseudouridine synthase, RluC/RluD	TIGRFAM	TIGR00005	TIGR00005	coord: 77..420 e-value: 7.0E-75 score: 250.0
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 151..338 e-value: 2.9E-29 score: 102.2
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 152..393 e-value: 8.35169E-64 score: 201.797
IPR036986	RNA-binding S4 domain superfamily	GENE3D	3.10.290.10		coord: 63..132 e-value: 1.5E-15 score: 58.4
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 134..408 e-value: 2.1E-67 score: 228.9
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 76..421
None	No IPR available	PANTHER	PTHR21600:SF57	RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE D	coord: 76..421
None	No IPR available	PROSITE	PS50889	S4	coord: 77..150 score: 12.251314
None	No IPR available	SUPERFAMILY	55174	Alpha-L RNA-binding motif	coord: 76..164
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 224..238
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 137..420

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G6901	Cucsat.G6901	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6901.T6.E1	Cucsat.G6901.T6.E1	exon
Cucsat.G6901.T6.E2	Cucsat.G6901.T6.E2	exon
Cucsat.G6901.T6.E3	Cucsat.G6901.T6.E3	exon
Cucsat.G6901.T6.E4	Cucsat.G6901.T6.E4	exon
Cucsat.G6901.T6.E5	Cucsat.G6901.T6.E5	exon
Cucsat.G6901.T6.E6	Cucsat.G6901.T6.E6	exon
Cucsat.G6901.T6.E7	Cucsat.G6901.T6.E7	exon
Cucsat.G6901.T6.E8	Cucsat.G6901.T6.E8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6901.T6.C1	Cucsat.G6901.T6.C1	CDS
Cucsat.G6901.T6.C2	Cucsat.G6901.T6.C2	CDS
Cucsat.G6901.T6.C3	Cucsat.G6901.T6.C3	CDS
Cucsat.G6901.T6.C4	Cucsat.G6901.T6.C4	CDS
Cucsat.G6901.T6.C5	Cucsat.G6901.T6.C5	CDS
Cucsat.G6901.T6.C6	Cucsat.G6901.T6.C6	CDS
Cucsat.G6901.T6.C7	Cucsat.G6901.T6.C7	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G6901.T6	Cucsat.G6901.T6-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:0000455	enzyme-directed rRNA pseudouridine synthesis
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding