Cucsat.G6901.T11 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G6901.T11
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPseudouridine synthase
Locationctg1522: 100671 .. 104589 (+)
RNA-Seq ExpressionCucsat.G6901.T11
SyntenyCucsat.G6901.T11
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCCCGGTAAGAAAACAGACAGCCGATTAGTGAAAACCCAGAAACGAAACTATCGAAGACGGGAGAATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTAGCGTCATTTTCCATAGAGAAGCTACATTTGCTAACCTTTTTTTTTTTCTTTCTATTTAGCTTCTTTCAGCTATTTTAGTATGATTATCTAGTGGTCTGTAATCGGAGTGGTGAATTATGTTCAGCCTATATTTACTGTTTTTGTTCCATATTTGAGTTCTTAATTTGTGTGTTCATTTGCAACTTCTATATATTTTTTAAATACTCAAGCAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTAATGATTCAAAAAAGTTTAATATTGTTATTATCAATACTATTTGAAGTTATTTGTGGATGCAAGATATCGATACTTGTGGATAAAATTAAACGCTCTCCCCTCCCACGCCTGAAGTTTTTAGTAAGATTGAAATTTGCACGTATCTGTGTTAGCGGTTTTCTTCATGATTCAGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGTGATTTGCTCTCCCTTTTTAAGCTTCCTTGTTACTGCTCATCTGAAGATTAAAGCAGACAATTCAAGTTTGGTGCTTCAAAAGTTTGCTACTCAAACACTTGTAATATCATGTTTAACTTCTAAGTTTTTTTCATTGGAACTACAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGGTAATTAGTTTTTCTGCATCTTTTATTTTATTTATGTACATAATTATTTTTCAGTGATAGAAAATATTTAAATATCACTGATTTTGGGCGTTCCTAACGCAAAGTTGAAACACCGAATGTTTCGCAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGGTGCGTAGTCTAAATGTCTTTCTCCTTAGGCTGTTTCAAATGACCAAAAAAAAAATTATATTACAATCTATCAATAGTCAGTTTAGAACATAAATTATATTCCCTAACACCACGCATATGTGCAAAGACATACATTTCTTTTGTTTGTAAAAGATTTCTCTATGATACTGCTACTGTTTTCCAGTCTTCATATGTGCATATGTAAAATAAAATTTCAATATAACGAGTCAGAGTTGCACCTCGAGCATACAAGCAGCCACATTTACCATTCCACATCCACTGCAATTTCAGTTCTTATCTTTTTTTAAAAAAAATTGAAATGCAGTTTTATTTTACTCGGTCTTAGGATTGAATATTTGTGAAGTAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTAGAAAACAAATCTTTAAGAGCTCATTTAGTCCGTTCTTATTTCATTTACTATCCATTTTTTGAGAAATATGTGATTTTGACCTTTCTTCTTTATTTTATTTTATTTATTCTTTTCTAAAATAGTTTATAACTTTCATCTCTTATTTCTAATGTTTTGAAACTAAATGGAATTTATATTTGTATTATTTTCCTCTAATAATAATTGTATGCAAAATATCAAAACATTAATTTGACTATTCAATTCCAATTTTTCGTTTAAATTTCCACTTTTTGAAAAAGAAACGTTGTAGAGAAAAATAAATGATAACAACTGCTAACCACATTTTTGTTCTTTTTTAATAAAAATGGAGAAAAGCAAGAAATGGTATAGAAACATGTTTTCACTTTTTACAATAAAAGAGACTAATGAATCTTTACTTGTTCATTCCTTCCGTAAACAACTAATTCAAGATCACTGAATATTTTGTAGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGGTATCCCTTATTTTGATTTCTTTCATTTTATAAGTTGAGCCAAGCTTTTTGCTTGGTAATCGGTTATTTTACAGATTTAATATCCATCCATCATCATGAAGAACAATATAAAAAATGTCCCGAGGGATACAATTGACACAACCAACCTCATTCGTAGCAACCTAGGTAGTTAATACGTGTCACCTTTATGCCTATTTTCTGGGCGCCCCCTTGCCATGTTATAGCTAATACCGGGTCTTCTCTATTTACTAGCTATTTTTCACCTGGTTCTTGTGTTTGGAGTGACTATTTGTTGGTTTAGTTTCCTTTTGTACTTCATCGCTAACTTATTCTACTAAACTTAACACAAAAATAAGTTGTTTAATGATGGGTGAAAATTTAGCAATACATAAAATATATTTTCTTATTATTTAAACGATGGTTTTATACATGAAAACATTGGAGACATGATAGATATAACATAATAACTAAATAGCAGACAAACAAGCATAGAAGCTCTCCTTTTACTTGCTTCTCTTATAAATAGTAACGAATAGGGACGTTTGTTCTATCTATACAAATGACATAATGGGGATAAATTCCCTGAACAATTTTGAACTCTATATCATGTGGAAATTTCGCATCATATCTTGTGGATTAATCTTATGCCGGTCTGATGGTAGATAGGGCAAATTTGAAATAATTTTGAGCTTCACAAAATATATTAGATGTTGAACAATGGTTACTACCATAGAATCTAGGTGAGATGTGGTTTTTACGGTGCCCCAGTTAACCTATAATAGAGTGTTGTCATAAAAGTGGCAGGTTACCAAATCTTAGCTGGGAGCCTCCAGTATCTGGCAAGTAGACTCGTCAAATGACTCGAAAATGAGATATCAAGAAGGTACATTAGGACATACAGCCATTTAAGTTGTGATGTCTGAAGCACAAATTTAAGTTCTGGGTAACTTACATATCTTGTTTATTAGATTCATTTAATTGTTGAAATTTAAAGTATTTTGTCACACATTCCCAGACGTTGAATTCCACGTATTGTTAATCTCTGCCATTAACTTCGATTATTGAAACC

Coding sequence (CDS)

ATGATTTCCGTTTGCTCCGCTAGAACAATTCAAGCTTCACCTTCTCCTATTCACTTCTTTTTCCCTCCAAATCGTAGAAGAATCGGTTCGAATGTTCTTCGAATTTCCTCCAATTTCTCCACTAGATGCTCCTCCGATTACGATGAATATAGTGAAGCTCAATCCGAACGGATAGCCAATTACGCCGGAACCAGACTGGAAGAGACCGTGGACGTCAATCCCGGTAAAGTTCGTCTCGATTCTTGGATTTCTTGCCGCATTAACGGCATCAGTAGGGCTCGAGTTCAGTCCAGTATTAGAGCGGGATTAGTTTCAGTTAATGGCCGAGTTATCGATAAGGTTTCTCACAATGTAAAAGCTGGGGATGTGATCAATTGTACCATATCCGAGTTGCAACCATTGAGAGCTGAACCTGAAGATATTGATTTGGATATAGTTTATGAAGATGAACATGTTTTAGTCGTAAACAAACCTGCTCACATGGTTGTTCATCCAGCACCTGGAAATGCGACAGGAACACTAGTAAATGCCATTCTTAACCACTGCAGTCTCCCGATGGTTGCAACATCAACTGGGGAAAATGAGTCGGATTCAGATGTTTCCGATGATGAGTTCAGTTCAATTGGAGTTTTTTCTAGAGTTTCTGAGGCGCCTATTCGTCCTGGTATTGTGCATAGGTTAGATAAAGGAACTAGTGGATTGCTTGTTGTTGCCAAGGATGAACATTCCCATGCTCACTTATCTGCTCAATTTAAGCAACATTCTATTGAGAGAGTTTATATTAGTCTCACATGTGGAGTTCCACCTTCAACTGCTGGGCGTATTGAGATGCCAATTTGCCGTGATCCAAACAATCGTATCCGCATGACTGCAATAGATGGAACAAAAAACAATCCACATGCAAAGTACGCTGCTAGTAGATACAATGTTATGGAAATTTTAGCCCAAGGAGGTTCTTCATTGGTTGAGTGGAGATTAGAAACAGGCCGCACACATCAGATACGTGCACACGCGAAGTATATGGGAATACCATTACTTGGAGACGAGGTCTATGGAGGTACCAAGAGCATGGCCATGTCGCTGCTTCGAACTAGAGCATCATCTAATTGTCACGGCCAGCTCATGCAGCTTGTTTCTTCATTAGAGAGGCCCTGTCTTCATGCTCTGACTCTTGGGTTTGTACATCCTCACACAGGGAAGAACATACGTTTTTCATGCCCACCCCCAACAGATTTTACTGAAATTTTGAGTCAGCTTCGCGAAATTGGCACACAAAAGTGGCAGGTTACCAAATCTTAG

Protein sequence

MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIGTQKWQVTKS
Homology
[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match: Q3ECD0 (RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76050 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-142
Identity = 268/429 (62.47%), Postives = 317/429 (73.89%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 362
                 A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYGGT
Sbjct: 311 GVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGT 370

Query: 363 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTE 422
           KSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D  E
Sbjct: 371 KSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDLAE 429

Query: 423 ILSQLREIG 424
           I+  LR  G
Sbjct: 431 IVGLLRRSG 429

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match: Q5Z8P2 (RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0717400 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.7e-137
Identity = 259/429 (60.37%), Postives = 313/429 (72.96%), Query Frame = 0 

Query: 7   ARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRC-SSDYDEYSEAQSERIANYAGTR 66
           A T  ASP  I        RR      R       RC ++D +  + A S R   + GTR
Sbjct: 2   ATTAAASPPAIATALSALLRRQRRRSSRCVGASHARCLAADANAEAVAPSRR-GGHGGTR 61

Query: 67  LEETVDVNPGKVRLDSWISCRI--NGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDV 126
           LEE V    G+ R+D+WIS R+   G+SRAR+Q+SIRAGLV VNGR + KVSH VK GD+
Sbjct: 62  LEEAVPAGEGRSRIDAWISARLGGGGVSRARIQASIRAGLVVVNGRPVSKVSHMVKGGDI 121

Query: 127 INCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCS 186
           ++CT+ ELQPLRAEPEDI LDIVYED+H+LVVNKPAHMVVHPAPGNA GTLVNAIL+HC 
Sbjct: 122 VSCTVLELQPLRAEPEDIPLDIVYEDDHLLVVNKPAHMVVHPAPGNANGTLVNAILHHCK 181

Query: 187 L---PMVATSTGENE------SDSDVSD-DEFSSIGVFSRVSEAPIRPGIVHRLDKGTSG 246
           +     +A ++ ++E       D DV D D+F++  V S V EA +RPGIVHRLDKGTSG
Sbjct: 182 ISTFTCLARNSIDDECPDSSDDDIDVFDIDQFTTGEVSSEVREALVRPGIVHRLDKGTSG 241

Query: 247 LLVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAID 306
           LLVVAKDEHSHA L+ QFK H+I RVYISLTCG P   +GRIE+PI RDPNNRIRM A  
Sbjct: 242 LLVVAKDEHSHAQLAEQFKLHTIRRVYISLTCGAPNPNSGRIEVPIARDPNNRIRMIATP 301

Query: 307 GTKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGG 366
           G+  + +A++AASRY V E+ A GGS+LVEWRLETGRTHQIRAHAKY+GIPLLGDE YGG
Sbjct: 302 GS-GHRYARHAASRYKVREVFAGGGSALVEWRLETGRTHQIRAHAKYLGIPLLGDETYGG 361

Query: 367 TKSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFT 423
           TKSMA+SLLR R  S  H  L  ++S ++RPCLHA  LGF HPH+GK + FSCPPP DFT
Sbjct: 362 TKSMALSLLRPRTPSRYHCDLSNMISKIDRPCLHAALLGFKHPHSGKILEFSCPPPDDFT 421

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match: P50513 (Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=rluD PE=3 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-54
Identity = 136/343 (39.65%), Postives = 185/343 (53.94%), Query Frame = 0 

Query: 78  RLDSWISCRINGISRARVQSSIRAGLVS-VNGRVIDKVSHNVKAGDVINCTISELQPLRA 137
           RLD  ++  I  +SR R+++ I +G VS   G ++   +  +K+ D     I   +P   
Sbjct: 16  RLDRALASLITRLSRERLKNLISSGCVSNSQGALVRDPAFKIKSLDCFTVDIPLPRPAHN 75

Query: 138 EPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENES 197
           EP+DI L+IV+EDEH+LVVNKPA MVVHPA GN   TLVNA+L HC+             
Sbjct: 76  EPQDIPLEIVFEDEHLLVVNKPAGMVVHPAAGNYDNTLVNALLYHCA------------- 135

Query: 198 DSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSI 257
                  + S IG          RPGIVHR+DK TSGLLVVAK + +HA L+AQF  HSI
Sbjct: 136 ------GKLSGIG-------GVARPGIVHRIDKDTSGLLVVAKTDPAHAGLAAQFADHSI 195

Query: 258 ERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQ 317
            R Y ++  G  PS  G ++ P+ R   NR +M  +    ++   K A + Y ++  L  
Sbjct: 196 NRRYRAIVDG-HPSLQGHVDAPLARSSVNRQKMAIV----SDGRGKRAVTHYRMITPLK- 255

Query: 318 GGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQ 377
             +SL+E RLETGRTHQ+R H   +G  LLGD VYG +K    +LL++ A          
Sbjct: 256 -NASLIECRLETGRTHQVRVHMSSIGHSLLGDPVYGRSKKAHHALLQSLA---------- 311

Query: 378 LVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420
                +R  LHA  LGF+HP +GK + F    P D   +   L
Sbjct: 316 ----FQRQALHAAHLGFIHPISGKQVDFDAEMPQDMQLLFKML 311

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match: Q45480 (Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168) OX=224308 GN=ylyB PE=3 SV=3)

HSP 1 Score: 213.4 bits (542), Expect = 5.1e-54
Identity = 132/346 (38.15%), Postives = 183/346 (52.89%), Query Frame = 0 

Query: 78  RLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAE 137
           R+D +++   N  SR +VQ  ++ G V VNG  + K ++ ++ GD +  T+ E + L   
Sbjct: 16  RIDKFLASTENDWSRTQVQQWVKDGQVVVNGSAV-KANYKIQPGDQVTVTVPEPEALDVL 75

Query: 138 PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESD 197
            E +DLDI YED+ VLVVNKP  MVVHPAPG+ TGTLVN ++ HC+              
Sbjct: 76  AEPMDLDIYYEDQDVLVVNKPRGMVVHPAPGHLTGTLVNGLMAHCT-------------- 135

Query: 198 SDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQHSIE 257
                 + S I          +RPGIVHR+DK TSGLL+VAK++ +H  L  Q    ++ 
Sbjct: 136 ------DLSGI-------NGVMRPGIVHRIDKDTSGLLMVAKNDMAHESLVNQLVNKTVT 195

Query: 258 RVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQG 317
           R Y ++  G+     G I+ PI RD  +R  MT     KN      A + ++V+E     
Sbjct: 196 RKYTAVVHGLISHDDGTIDAPIGRDKKDRQSMTVTRDGKN------AVTHFHVLERFQD- 255

Query: 318 GSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRASSNCHGQLMQL 377
             +LVE +LETGRTHQIR H KY+G PL GD  YG  K++           + +GQ    
Sbjct: 256 -FTLVECQLETGRTHQIRVHMKYIGFPLAGDPKYGPRKTI-----------DFNGQ---- 303

Query: 378 VSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIG 424
                   LHA  LGF HP TG+ + F  P P D  E++  LR+ G
Sbjct: 316 -------ALHAGVLGFDHPRTGEYVEFEAPLPEDMAELIENLRKNG 303

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match: O50310 (Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=Cpar_0723 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.1e-51
Identity = 132/358 (36.87%), Postives = 195/358 (54.47%), Query Frame = 0 

Query: 69  TVDVNPGK--VRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKAGDVINC 128
           T+DV+  +  +R+D +++ ++  ++R +VQ++I  G V VNG+ + K ++ VK+ D I+ 
Sbjct: 2   TLDVSKVQTPMRIDRYLTQQVENMTRNKVQAAIEEGRVLVNGKPV-KSNYRVKSCDHIHL 61

Query: 129 TISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILNHCSLPM 188
           T          PEDI + I+YED+ ++V++K A MVVHPA GN TGTL NAIL+H     
Sbjct: 62  TFLRPPAPELAPEDIPITILYEDDDLMVIDKEAGMVVHPAFGNWTGTLANAILHHLG--- 121

Query: 189 VATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKDEHSHAH 248
                     D+D  D             +  +RPGIVHRLDK TSGL+++AK+  +   
Sbjct: 122 ---------KDADELD-------------KTEMRPGIVHRLDKDTSGLIIIAKNPTALHK 181

Query: 249 LSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPHAKYAAS 308
           L+ QF    +E+VY ++  GVP + +G I+  I R   NR  M   +        K+A +
Sbjct: 182 LARQFANRQVEKVYKAIVWGVPKAESGTIKTNIGRSHKNRKVMA--NFPYEGAEGKHAIT 241

Query: 309 RYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMSLLRTRA 368
            Y V+E L  G  SL++  L TGRTHQIR H +++G  +LGDE YGG         RT  
Sbjct: 242 DYQVVEDL--GYFSLMDVTLHTGRTHQIRVHLQHLGHSILGDETYGGATP------RTLP 301

Query: 369 SSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLREIGT 425
            S        L+  + R  LHA TL F  P TG+ + FS P P D   +L ++R + T
Sbjct: 302 FSKSEPFTRNLLELMSRQALHAQTLRFRQPTTGEPLSFSAPLPEDMELVLEKIRTVMT 323

[Close]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match: KGN62268.1 (hypothetical protein Csa_018737 [Cucumis sativus])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGTQKWQVTKS
Sbjct: 421 EIGTQKWQVTKS 432

[Close]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match: XP_031736623.1 (RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 854 bits (2207), Expect = 2.73e-312
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQK 426
           EIGTQK
Sbjct: 421 EIGTQK 426

[Close]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match: XP_008445318.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo] >XP_008445319.1 PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 820 bits (2118), Expect = 5.67e-299
Identity = 412/432 (95.37%), Postives = 421/432 (97.45%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGT+K QVTKS
Sbjct: 421 EIGTKKLQVTKS 431

[Close]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match: XP_008445320.1 (PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 814 bits (2102), Expect = 1.44e-296
Identity = 411/432 (95.14%), Postives = 420/432 (97.22%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGT+K  VTKS
Sbjct: 421 EIGTKK--VTKS 429

[Close]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match: XP_038886062.1 (RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 765 bits (1975), Expect = 5.69e-277
Identity = 383/426 (89.91%), Postives = 399/426 (93.66%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS I  FFP NRRRI  +VLR S N STR SSDYDEY EAQSER AN
Sbjct: 1   MISVCSARTIQASPSSIQLFFPRNRRRITPDVLRFSVNLSTR-SSDYDEYGEAQSERRAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           +AG RLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGR+IDKVSHNVKA
Sbjct: 61  FAGIRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRIIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRAEPEDIDLDIVYED HVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDMINCTISELQPLRAEPEDIDLDIVYEDAHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATS GE  SD D+SDDEFSS+ +FSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSKGELPSDMDISDDEFSSVEIFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFK HSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA+ GT NNPH
Sbjct: 241 EHSHAHLSAQFKLHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMTAVSGTNNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRY VMEILAQGGSSLVEWRLETGRTHQIRAHAK+MGIPLLGD+VYGGTKSMAMS
Sbjct: 301 AKHAASRYKVMEILAQGGSSLVEWRLETGRTHQIRAHAKHMGIPLLGDDVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLR+R SS+CH QLMQ +SSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRSRTSSSCHSQLMQHISSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQK 426
           EIGT+K
Sbjct: 421 EIGTKK 425

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match: A0A0A0LKD7 (Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1)

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGTQKWQVTKS
Sbjct: 421 EIGTQKWQVTKS 432

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match: A0A1S3BCE4 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 820 bits (2118), Expect = 2.75e-299
Identity = 412/432 (95.37%), Postives = 421/432 (97.45%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGT+K QVTKS
Sbjct: 421 EIGTKKLQVTKS 431

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match: A0A1S3BD56 (Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1)

HSP 1 Score: 814 bits (2102), Expect = 6.99e-297
Identity = 411/432 (95.14%), Postives = 420/432 (97.22%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQLR 420
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDF EILSQLR
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFAEILSQLR 420

Query: 421 EIGTQKWQVTKS 432
           EIGT+K  VTKS
Sbjct: 421 EIGTKK--VTKS 429

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match: A0A5A7VHA3 (Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001610 PE=3 SV=1)

HSP 1 Score: 742 bits (1916), Expect = 5.13e-269
Identity = 375/392 (95.66%), Postives = 383/392 (97.70%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCSARTIQASPS IHFFFP NRRRI SNVLR S+NFS+RCS DYDEYSEAQSERIAN
Sbjct: 1   MISVCSARTIQASPSSIHFFFPRNRRRIDSNVLRFSANFSSRCS-DYDEYSEAQSERIAN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAGTRLEETVD+NPGKVRLDSWIS RINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA
Sbjct: 61  YAGTRLEETVDINPGKVRLDSWISSRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240
           HCSLPMV+TSTGE++SDSDVSDDEFSSI VFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD
Sbjct: 181 HCSLPMVSTSTGEHQSDSDVSDDEFSSIEVFSRVSEAPIRPGIVHRLDKGTSGLLVVAKD 240

Query: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNPH 300
           EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRMTA  GTKNNPH
Sbjct: 241 EHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEIPICRDPNNRIRMTATHGTKNNPH 300

Query: 301 AKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360
           AK+AASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS
Sbjct: 301 AKHAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAMS 360

Query: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 392
           LLRTRASSNCHGQLMQLVSSLERPCLHALTLG
Sbjct: 361 LLRTRASSNCHGQLMQLVSSLERPCLHALTLG 391

[Close]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match: A0A6J1CMS3 (Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 1.49e-261
Identity = 371/433 (85.68%), Postives = 394/433 (90.99%), Query Frame = 0 

Query: 1   MISVCSARTIQASPSPIHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSERIAN 60
           MISVCS R IQA+PS I  FFP NR RI SNV RI++N STRCS DYDE  EA SERI N
Sbjct: 1   MISVCSVRAIQAAPS-IQLFFPRNRGRITSNVHRIATNHSTRCS-DYDECGEAHSERITN 60

Query: 61  YAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHNVKA 120
           YAG RLEE VDVN GK+RLDSWISCRINGISRARVQSSIRAGLV+VNGRVIDKVSHN+KA
Sbjct: 61  YAGMRLEEIVDVNSGKIRLDSWISCRINGISRARVQSSIRAGLVTVNGRVIDKVSHNIKA 120

Query: 121 GDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180
           GD+INCTISELQPLRA+PEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN
Sbjct: 121 GDMINCTISELQPLRAKPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNAILN 180

Query: 181 HCSLPMVATSTGENESDSDVSDDEFSSIGVFS-RVSEAPIRPGIVHRLDKGTSGLLVVAK 240
           HCSLPMVATS GE  SD D+SDDEFSS  + S R SEAPIRPGIVHRLDKGTSGLLVVAK
Sbjct: 181 HCSLPMVATSNGEL-SDMDISDDEFSSNEILSSRASEAPIRPGIVHRLDKGTSGLLVVAK 240

Query: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDGTKNNP 300
           DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIE+PICRDPNNRIRM+A+  T  NP
Sbjct: 241 DEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEVPICRDPNNRIRMSAVLRTNKNP 300

Query: 301 HAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAM 360
           HAKYAASRY V+EILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMA+
Sbjct: 301 HAKYAASRYKVIEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSMAL 360

Query: 361 SLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTEILSQL 420
           SLLRTR SS+CH QLMQLVS+LERPCLHALTLGF+HP+TG+NIRFSCPPPTDF +I SQL
Sbjct: 361 SLLRTRTSSSCHSQLMQLVSALERPCLHALTLGFIHPYTGENIRFSCPPPTDFADIYSQL 420

Query: 421 REIGTQKWQVTKS 432
            EIGT+K  VT+S
Sbjct: 421 CEIGTKK--VTES 428

[Close]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 9.3e-144
Identity = 268/429 (62.47%), Postives = 317/429 (73.89%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASRYNVMEILAQGGSSLVEWRLETGRTHQIRAHAKYMGIPLLGDEVYGGT 362
                 A++AASRY V+E  A GGS+LVEWRLETGRTHQIRAHAKYMG+PLLGDEVYGGT
Sbjct: 311 GVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGT 370

Query: 363 KSMAMSLLRTRASSNCHGQLMQLVSSLERPCLHALTLGFVHPHTGKNIRFSCPPPTDFTE 422
           KSMA+SLL+ R S +   ++++L+S ++RPCLHA+ LGF HP TG+ ++FSCPPP+D  E
Sbjct: 371 KSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPSDLAE 429

Query: 423 ILSQLREIG 424
           I+  LR  G
Sbjct: 431 IVGLLRRSG 429

[Close]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match: AT1G76050.1 (Pseudouridine synthase family protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-93
Identity = 192/313 (61.34%), Postives = 222/313 (70.93%), Query Frame = 0 

Query: 3   SVCSARTIQASPSP-----IHFFFPPNRRRIGSNVLRISSNFSTRCSSDYDEYSEAQSER 62
           S+ +A++++   SP     I F F P      SN +R SS      +    E    +   
Sbjct: 11  SLTTAKSLRYPSSPSSSLSIFFSFFPK----VSNFVRASSGIPNLVACSPTEIIIPR--- 70

Query: 63  IANYAGTRLEETVDVNPGKVRLDSWISCRINGISRARVQSSIRAGLVSVNGRVIDKVSHN 122
             N AG R+EE VD   GK+RLDSWIS RING+SRARVQSSIR GLV+VNGRV+DKVSHN
Sbjct: 71  -VNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLGLVTVNGRVVDKVSHN 130

Query: 123 VKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVVHPAPGNATGTLVNA 182
           VK+GD +NCTISELQPL+AE EDI LDIVYED+HVLVVNKPAHMVVHPAPGN TGTLVN 
Sbjct: 131 VKSGDEVNCTISELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNG 190

Query: 183 ILNHCSLPMVATSTGENESDSD---VSDDEFSSIGVFSRVSEAPIRPGIVHRLDKGTSGL 242
           IL+HCSLP V  S  E + DSD    SDDE  +    S    A +RPGIVHRLDKGT+GL
Sbjct: 191 ILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYA--ASVRPGIVHRLDKGTTGL 250

Query: 243 LVVAKDEHSHAHLSAQFKQHSIERVYISLTCGVPPSTAGRIEMPICRDPNNRIRMTAIDG 302
           LVVAKDEHSHAHL+ QFK H+IERVY+SLT GVP    GRIE+PI RD +NRIRM AI G
Sbjct: 251 LVVAKDEHSHAHLAEQFKLHTIERVYVSLTTGVPSPPQGRIEIPIGRDSSNRIRMAAIPG 310

Query: 303 TKNNPHAKYAASR 308
                 A++AASR
Sbjct: 311 GVRGGRARHAASR 313

[Close]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-14
Identity = 70/272 (25.74%), Postives = 119/272 (43.75%), Query Frame = 0 

Query: 99  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 158
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 159 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAP 218
           + + V P                  L  +    G+N+S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 219 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 278
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 279 SLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGS 338
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 295

Query: 339 SLVEWRLETGRTHQIRAHAKYMGIPLLGDEVY 352
           SLV+  +++GR HQIR H  YMG PL+GD +Y
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPLVGDPLY 295

[Close]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.8e-12
Identity = 67/266 (25.19%), Postives = 114/266 (42.86%), Query Frame = 0 

Query: 99  IRAGLVSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKP 158
           I+ G + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP
Sbjct: 56  IQNGQIQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKP 115

Query: 159 AHMVVHPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAP 218
           + + V P                  L  +    G+N+S             + SR S  P
Sbjct: 116 SGLQVLPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHP 175

Query: 219 IRPGIVHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYI 278
           +    VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y 
Sbjct: 176 VP---VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYR 235

Query: 279 SLTCGVPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGS 338
           +L  G+       I+ PI   R P       A      +P  K A S+  V+E   +   
Sbjct: 236 ALADGIVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNC 289

Query: 339 SLVEWRLETGRTHQIRAHAKYMGIPL 346
           SLV+  +++GR HQIR H  YMG PL
Sbjct: 296 SLVKVEIQSGRPHQIRIHLAYMGHPL 289

[Close]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.8e-12
Identity = 66/273 (24.18%), Postives = 118/273 (43.22%), Query Frame = 0 

Query: 104 VSVNGRVIDKVSHNVKAGDVINCTISELQPLRAEPEDIDLDIVYEDEHVLVVNKPAHMVV 163
           + ++G V+   +  +++G  +   +    P +       L+++YED+ ++ +NKP+ + V
Sbjct: 91  IQIDGEVVKDPNTLLRSGSKL---VYSRLPWKEPDTPYSLEVLYEDDDLIALNKPSGLQV 150

Query: 164 HPAPGNATGTLVNAILNHCSLPMVATSTGENESDSDVSDDEFSSIGVFSRVSEAPIRPGI 223
            P                  L  +    G+N+S             + SR S  P+    
Sbjct: 151 LPG---------GLFQQRTVLTQLQWCFGKNDS------------YIGSRESPHPVP--- 210

Query: 224 VHRLDKGTSGLLVVAKDEHSHAHLSAQFKQ-----------------HSIERVYISLTCG 283
           VHRL +GTSG+L+ AK + +   L+A F +                   + ++Y +L  G
Sbjct: 211 VHRLGRGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADG 270

Query: 284 VPPSTAGRIEMPI--CRDPNNRIRMTAIDGTKNNPHAKYAASRYNVMEILAQGGSSLVEW 343
           +       I+ PI   R P       A      +P  K A S+  V+E   +   SLV+ 
Sbjct: 271 IVEEDEVVIKQPIGVVRYPG-----VAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKV 330

Query: 344 RLETGRTHQIRAHAKYMGIPLLGDEVYGGTKSM 358
            +++GR HQIR H  YMG PL+   ++ G++ +
Sbjct: 331 EIQSGRPHQIRIHLAYMGHPLVEMTMFTGSECL 331

The following BLAST results are available for this feature:
BLAST of Cucsat.G6901.T11 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3ECD01.3e-14262.47RNA pseudouridine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q5Z8P21.7e-13760.37RNA pseudouridine synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
P505131.3e-5439.65Ribosomal large subunit pseudouridine synthase D OS=Zymomonas mobilis subsp. mob... [more]
Q454805.1e-5438.15Uncharacterized RNA pseudouridine synthase YlyB OS=Bacillus subtilis (strain 168... [more]
O503103.1e-5136.87Uncharacterized RNA pseudouridine synthase Cpar_0723 OS=Chlorobaculum parvum (st... [more]
BLAST of Cucsat.G6901.T11 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN62268.10.0100.00hypothetical protein Csa_018737 [Cucumis sativus][more]
XP_031736623.12.73e-312100.00RNA pseudouridine synthase 2, chloroplastic [Cucumis sativus][more]
XP_008445318.15.67e-29995.37PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X1 [Cucumis melo]... [more]
XP_008445320.11.44e-29695.14PREDICTED: RNA pseudouridine synthase 2, chloroplastic isoform X2 [Cucumis melo][more]
XP_038886062.15.69e-27789.91RNA pseudouridine synthase 2, chloroplastic isoform X4 [Benincasa hispida][more]
BLAST of Cucsat.G6901.T11 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKD70.0100.00Pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G346580 PE=3 SV=1[more]
A0A1S3BCE42.75e-29995.37Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A1S3BD566.99e-29795.14Pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103488386 PE=3 SV=1[more]
A0A5A7VHA35.13e-26995.66Pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A6J1CMS31.49e-26185.68Pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111012905 PE=3 SV=1[more]
BLAST of Cucsat.G6901.T11 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G76050.29.3e-14462.47Pseudouridine synthase family protein [more]
AT1G76050.11.2e-9361.34Pseudouridine synthase family protein [more]
AT3G52260.21.4e-1425.74Pseudouridine synthase family protein [more]
AT3G52260.13.8e-1225.19Pseudouridine synthase family protein [more]
AT3G52260.33.8e-1224.18Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002942RNA-binding S4 domainSMARTSM00363s4_6coord: 77..139
e-value: 7.5E-5
score: 32.1
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 77..124
e-value: 6.3E-9
score: 35.4
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 77..156
e-value: 4.20157E-13
score: 62.267
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 134..408
e-value: 2.0E-67
score: 228.9
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 76..421
NoneNo IPR availablePANTHERPTHR21600:SF57RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Dcoord: 76..421
NoneNo IPR availablePROSITEPS50889S4coord: 77..150
score: 12.251314
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 76..164
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 63..132
e-value: 1.4E-15
score: 58.4
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 151..338
e-value: 2.8E-29
score: 102.2
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 152..393
e-value: 4.7871E-64
score: 202.182
IPR006225Pseudouridine synthase, RluC/RluDTIGRFAMTIGR00005TIGR00005coord: 77..420
e-value: 6.8E-75
score: 250.1
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 224..238
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 137..420

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G6901Cucsat.G6901gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T11.E1Cucsat.G6901.T11.E1exon
Cucsat.G6901.T11.E2Cucsat.G6901.T11.E2exon
Cucsat.G6901.T11.E3Cucsat.G6901.T11.E3exon
Cucsat.G6901.T11.E4Cucsat.G6901.T11.E4exon
Cucsat.G6901.T11.E5Cucsat.G6901.T11.E5exon
Cucsat.G6901.T11.E6Cucsat.G6901.T11.E6exon
Cucsat.G6901.T11.E7Cucsat.G6901.T11.E7exon
Cucsat.G6901.T11.E8Cucsat.G6901.T11.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T11.C1Cucsat.G6901.T11.C1CDS
Cucsat.G6901.T11.C2Cucsat.G6901.T11.C2CDS
Cucsat.G6901.T11.C3Cucsat.G6901.T11.C3CDS
Cucsat.G6901.T11.C4Cucsat.G6901.T11.C4CDS
Cucsat.G6901.T11.C5Cucsat.G6901.T11.C5CDS
Cucsat.G6901.T11.C6Cucsat.G6901.T11.C6CDS
Cucsat.G6901.T11.C7Cucsat.G6901.T11.C7CDS
Cucsat.G6901.T11.C8Cucsat.G6901.T11.C8CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G6901.T11Cucsat.G6901.T11-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding