Lsi05G003030 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G003030
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Plastid movement impaired 2
Location	chr05 : 3878165 .. 3880617 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTTAGGCTACTAGAAAGAAATAGATTTTGTGTTAATAGGTCTAGATTTTTATATGTGACATGTATTAGATTGCTTTAAAGTCAACCAAAGTATAAGCCAAGTGATTACACATTGTATCTGCTTGTGATCGTGTTTGCATCTTCAAATAATTTTATTAAAATAATTGTTGAATGATATTTCAATCAATTTTGTGTTAACATATTCTTTTGAAAAAAACCTAATTGAAAATTTTTAATACATTAAAGAGATAAAAATGAAAATTTTTACATGATATATGTATGTAGAAATTTGAAGCCAAATGCAAGTGAAGAATATAACTCTTGAAAGCCAATTTTAACATTCTTATACCTTTTTTTGATCAAAGGTTTAAAGGTTTATGTATCTAAACTTGAGACTCTAGAATCTTTTCGATTGCTCGTTCGAGAATTACGATCTTTGGCTTAGGAACAAAATCATTTCCTTATATTTGAGAAGAATTTCCAGATTAATAGGAAGGAAAGCTTAATCAAAATGAATCAGAAAATTTTGTCTTTGATCGATTAGTATAAACATCAACAACTCCGAATTGGATTAGTTTCTCCTATCAACAAATAAGTGTTTGGACCAATAAAACCCTACATAATGGCAAGATAGTTGGAGAGGTAACAAAATCCTTTCTCACTCACCTCATATTATTGTACTAAAACCAAAATGTATTTTTCTTTATTCACTAATGAGGTCATGTTTGGGAGTGATTTAGAAACGACTAAAATCATTTTCGTTATTTTCAAAATCACTCTGTAACATATTTCAATTATTCAAAACCAAATTTGATGATATAAAAGTTACACTTAAAAGTGTAAAATTAAATATTAAATTAATTTTGAGTAATTAAAGATACGTTTTAGAGTAATTTTTAAATCCGACAAACATGATCTTAATAATTTCAAAAATCACTCACGAATATGCACTAAATCATCTCAAAAGTCGATAGTTTGATCTTTCTATACTGGTTGAGCTCGAAAAAGAAAAAAAAGCTCAAATTACATGTGTAAATGTCAAATGAGAGAGAGAGAGCATCAATTAAACTATTCAATTGTTCCCATCACACCTATAATATATATATATATATATATAAACACATAAGTAAAAACTCCAAACAAAAGCAACTTTGTGAAGATCAAAGAGATTGAGATACAAAAGAGAGAAAGAAAGAAAGATGGGAAATGCAATGGGAGTTAGAGGATTAAGAAGAAGAAAAAGAACAAAAGTGATGAAAATCAATGGAGAAACCTTCAAAATAACAATTCCAACCACAGCCTACGAAGTCACAAGCCATCATCCAAATCACTTTCTCTATGAATCCAAATCCCTCAAGCAATTTGGCCTCCGAGCCAAGCCTTTGAACCCTAACTACCAACTCAAACCTAAAACCCTATACTTCCTCCTTCAATTGCCGACCCTCCCCGGCCGCCACCGTCCGCTTCGTCGCGTTTGTTCCGACCTTCATTTGTCTGCCTCCGATCGCCTTGAGTGCCTCATGATCTCTCGTCGATCGGTTTCCGACCTCGAAACCCTTCGGTTCGATGCTCAGAGGTTCTCAACCGACGGTGGTGCCACTAGGCCGGTGCAGGTCAAATTTCGACTGTCGAGGGCGGAATTCGAGAGATTGATGAAGGAATGTAAGAACAAGGTGGAGGCAACGGAGAGAATTGTTGATCTTTTTGCAAAAAGTAGAGATAATGGCGATGGCGGCCAAGAGGATGAGTTCTTTTGGAAGATGGATGATGCTTGTAGTGGTAAAGCGCGCGAGGTGAGTTTGTTAGCTGTTTTATTTGAATTATCCTTCAATTTGATGATAAAATTGTTATAATTTTCTAAAATTCTACTAATTTGTAAATTTTTTATTTACTTTTTTGTAATTTTAAAAGTTTTAATTTGGTGGTTAAATCTTGGAAAATTATTATAAAGGAAAAAGATATCAAATTATTTATAAATATAGAAATTTTTCACTATATATCAGCGATAGACTTCTATCGCCAGTCTATCGCGGTCTATCACAGATAGATAGAGAAATTTTTCTATATTTGTAAATAGTTTGATTCATTTTTCTATATTTGAAAATAACCCTTAAATCTTTGATAATTTTCTTAATGAGCAATGATTAGATTCAGCAAAAACTACACATAATCCCATGTTCTCTTATTTTTTATACATAAAAAAAAAGACATTTAAAAAAGTATATAGTCACGTGGTTGCACAAAGATAGGTGCAGTCTAGACAACACCTAAGTTTTTCTCTTTCTTAATTACTTGACAAAGTATTTTTGAGACACCAACTCATTATTGCTTATGATCACTATTGTATATGAACATATGGGATGTTTGGAGCTAGAAGGAAAAGTTTGGTTTAGTGGAGTGGAGTTAACTCCTTATGTGCTTAAACTCACCGTGAACTCCACTCAATAA

mRNA sequence

ATGAATAGATTGAGATACAAAAGAGAGAAAGAAAGAAAGATGGGAAATGCAATGGGAGTTAGAGGATTAAGAAGAAGAAAAAGAACAAAAGTGATGAAAATCAATGGAGAAACCTTCAAAATAACAATTCCAACCACAGCCTACGAAGTCACAAGCCATCATCCAAATCACTTTCTCTATGAATCCAAATCCCTCAAGCAATTTGGCCTCCGAGCCAAGCCTTTGAACCCTAACTACCAACTCAAACCTAAAACCCTATACTTCCTCCTTCAATTGCCGACCCTCCCCGGCCGCCACCGTCCGCTTCGTCGCGTTTGTTCCGACCTTCATTTGTCTGCCTCCGATCGCCTTGAGTGCCTCATGATCTCTCGTCGATCGGTTTCCGACCTCGAAACCCTTCGGTTCGATGCTCAGAGGTTCTCAACCGACGGTGGTGCCACTAGGCCGGTGCAGGTCAAATTTCGACTGTCGAGGGCGGAATTCGAGAGATTGATGAAGGAATGTAAGAACAAGGTGGAGGCAACGGAGAGAATTGTTGATCTTTTTGCAAAAAGTAGAGATAATGGCGATGGCGGCCAAGAGGATGAGTTCTTTTGGAAGATGGATGATGCTTGTAGTGGTAAAGCGCGCGAGCTAGAAGGAAAAGTTTGGTTTAGTGGAGTGGAGTTAACTCCTTATGTGCTTAAACTCACCGTGAACTCCACTCAATAA

Coding sequence (CDS)

ATGAATAGATTGAGATACAAAAGAGAGAAAGAAAGAAAGATGGGAAATGCAATGGGAGTTAGAGGATTAAGAAGAAGAAAAAGAACAAAAGTGATGAAAATCAATGGAGAAACCTTCAAAATAACAATTCCAACCACAGCCTACGAAGTCACAAGCCATCATCCAAATCACTTTCTCTATGAATCCAAATCCCTCAAGCAATTTGGCCTCCGAGCCAAGCCTTTGAACCCTAACTACCAACTCAAACCTAAAACCCTATACTTCCTCCTTCAATTGCCGACCCTCCCCGGCCGCCACCGTCCGCTTCGTCGCGTTTGTTCCGACCTTCATTTGTCTGCCTCCGATCGCCTTGAGTGCCTCATGATCTCTCGTCGATCGGTTTCCGACCTCGAAACCCTTCGGTTCGATGCTCAGAGGTTCTCAACCGACGGTGGTGCCACTAGGCCGGTGCAGGTCAAATTTCGACTGTCGAGGGCGGAATTCGAGAGATTGATGAAGGAATGTAAGAACAAGGTGGAGGCAACGGAGAGAATTGTTGATCTTTTTGCAAAAAGTAGAGATAATGGCGATGGCGGCCAAGAGGATGAGTTCTTTTGGAAGATGGATGATGCTTGTAGTGGTAAAGCGCGCGAGCTAGAAGGAAAAGTTTGGTTTAGTGGAGTGGAGTTAACTCCTTATGTGCTTAAACTCACCGTGAACTCCACTCAATAA

Protein sequence

MNRLRYKREKERKMGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAKPLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETLRFDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDGGQEDEFFWKMDDACSGKARELEGKVWFSGVELTPYVLKLTVNSTQ

Homology

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BLAST of Lsi05G003030 vs. Swiss-Prot
Match: Y1648_ARATH (Uncharacterized protein At1g66480 OS=Arabidopsis thaliana GN=At1g66480 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.7e-29
Identity = 80/190 (42.11%), Postives = 115/190 (60.53%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++ V+    RKR KVMKI+GETF+I  P TA EVT+ +P + L +S+++K FG+R+K
Sbjct: 1   MGNSITVK----RKRAKVMKIDGETFRIKTPVTAREVTADYPGYVLLDSQAVKHFGVRSK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLP--------GRHRPLRRVCSDLHLSASDRLECLMISRR 133
           PL PN  LKPK  YFL++LP LP            P RRV S +H+ A +RL+ LM+SRR
Sbjct: 61  PLEPNQTLKPKKTYFLVELPKLPPETTAVDTENKLPYRRVMSGIHVGAKERLDMLMLSRR 120

Query: 134 SVSDLETLRFD-AQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEA-TERIVDLFA 193
           +VSD+   R D    F  + G      V+ RL R++  +LM+E  N   A  E+I+ ++ 
Sbjct: 121 TVSDVTIGRSDGGDGFGPELGPGH-TSVRLRLPRSQITKLMEENNNDASAIAEKILGIYM 180

[Close]

BLAST of Lsi05G003030 vs. TrEMBL
Match: A0A0A0L7W0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G368690 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-74
Identity = 152/198 (76.77%), Postives = 169/198 (85.35%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRT-KVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRA 73
           MGNA+G RG RR+KRT KVMKINGET KIT+PTTAYEVTSH+P+H LYESKSLK FGLRA
Sbjct: 1   MGNAIG-RGSRRKKRTTKVMKINGETIKITLPTTAYEVTSHYPSHLLYESKSLKLFGLRA 60

Query: 74  KPLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLH-LSASDRLECLMISRRSVSDLE 133
           KPL+P+  L+PKTLYFLLQLPTLP  H  LRR CSDLH LSASDRLECL++SRRS+SDL+
Sbjct: 61  KPLDPHCHLEPKTLYFLLQLPTLPRDHCSLRRTCSDLHNLSASDRLECLLLSRRSLSDLQ 120

Query: 134 TLRFDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDG 193
           TLRFD QR STD GA +PVQVKF+L RAEFERLMKECKNKVE T+RIVD  A+SRDN  G
Sbjct: 121 TLRFDPQRSSTDDGAAKPVQVKFQLPRAEFERLMKECKNKVEVTKRIVDYCARSRDNVYG 180

Query: 194 GQEDEFFWKMDDACSGKA 210
            Q+DE FWKM  A SGKA
Sbjct: 181 NQDDELFWKMGGAYSGKA 197

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BLAST of Lsi05G003030 vs. TrEMBL
Match: A0A067JIK5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02495 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.4e-35
Identity = 88/191 (46.07%), Postives = 123/191 (64.40%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++G     R K+ K+MK++GETFK+  P  A +V   +P H L +S ++K FG+RAK
Sbjct: 1   MGNSIG-----RGKKAKIMKVDGETFKLKTPAKAMDVVKDYPGHVLLDSDAVKHFGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPL---RRVCSDLHLSASDRLECLMISRRSVSDL 133
           PL P  +L PK +YFL++LP LP    P+   RRV S +HLSA DRLE LM+SRRSVSDL
Sbjct: 61  PLEPEMELNPKKIYFLVELPKLPEEKEPISITRRVRSGIHLSAKDRLEFLMLSRRSVSDL 120

Query: 134 ETLRFDAQRFSTDG-GATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNG 193
             +R    +   DG   + PV+VK RL +A+ E+L++E K++VE  E+IVDLF +     
Sbjct: 121 SIVRPLPSQIPDDGHNGSGPVRVKMRLPKAQMEKLVEESKDEVEVAEKIVDLFME----- 180

Query: 194 DGGQEDEFFWK 201
           + G  +   WK
Sbjct: 181 NSGDRNRRQWK 181

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BLAST of Lsi05G003030 vs. TrEMBL
Match: V4UVZ8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012690mg PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 8.2e-34
Identity = 84/180 (46.67%), Postives = 114/180 (63.33%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN +G      R++ KVMKI+GET K+  P  A EV   +P + L +S+++K FG+RAK
Sbjct: 1   MGNTIG-----GRRKAKVMKIDGETIKLKTPIQASEVVKDYPGYVLLDSEAVKHFGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETL 133
           PL P  QLKPK +YFL++LP LP   +  RRV S + +SA DRLECLM+SRR+VSDL  +
Sbjct: 61  PLEPRQQLKPKKVYFLVELPKLPDEEKITRRVRSGIQMSAKDRLECLMLSRRAVSDLTAV 120

Query: 134 RFDAQRFS--TDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDG 192
              +   S    G  T  ++VK RL RA+ E+LM+E  N  E  E+IVDL+ +    G G
Sbjct: 121 NVRSSHGSEVAPGPGTGQMRVKMRLPRAQVEKLMEESANDEEVAEKIVDLYVEKSGGGGG 175

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BLAST of Lsi05G003030 vs. TrEMBL
Match: A0A022QMX5_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a023844mg PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-33
Identity = 87/176 (49.43%), Postives = 114/176 (64.77%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++G      ++R KVMKINGETFK+  P  A  V   HP H L ES+S++  G RAK
Sbjct: 1   MGNSLG-----GKRRAKVMKINGETFKLKTPVQARSVLESHPGHVLLESESVRHHGARAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLET- 133
           PL P+++LKPK LYFL+QLP  P   + +RRV S +H+SA DRL+ LM++RRS SDL T 
Sbjct: 61  PLEPHHELKPKRLYFLVQLPKFP-EDKSVRRVRSGIHMSAKDRLDSLMLARRSASDLSTV 120

Query: 134 --LRFDAQR-----FSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDL 182
             +RF+ +        T GG  R V+V  RL ++E ERLM E K+  EA E+IV L
Sbjct: 121 KPVRFEIEEERKGILETRGGGGRGVRVTMRLPKSEVERLMAESKDGAEAAEKIVSL 170

[Close]

BLAST of Lsi05G003030 vs. TrEMBL
Match: A0A061EH62_THECC (Plastid movement impaired 2 OS=Theobroma cacao GN=TCM_019256 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.4e-33
Identity = 80/178 (44.94%), Postives = 116/178 (65.17%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN+MG      RK+ KVMKI+GETFK+  P  A++V   +P H L +S+++K FG+RAK
Sbjct: 1   MGNSMG-----GRKKAKVMKIDGETFKLKTPVRAWDVVKDYPGHVLLDSEAVKHFGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETL 133
           PL P   LKPK +YFL++LP  P    P R     +H+SA DRLECLM+SRRSVSDL  +
Sbjct: 61  PLEPQQDLKPKKIYFLVELPKFPEEKAPRRVRSGGIHMSAKDRLECLMLSRRSVSDLSMV 120

Query: 134 RFDAQRFSTD-GGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGD 191
           R  +   S   G ++  + VK RL +++  +L++E ++ VE  E+I+DL+ ++   GD
Sbjct: 121 RPSSSLGSDGLGRSSGGMTVKMRLPKSQMAKLVEESRDGVEVAEKILDLYVENAGGGD 173

[Close]

BLAST of Lsi05G003030 vs. TAIR10
Match: AT1G66480.1 (AT1G66480.1 plastid movement impaired 2)

HSP 1 Score: 129.0 bits (323), Expect = 3.8e-30
Identity = 80/190 (42.11%), Postives = 115/190 (60.53%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++ V+    RKR KVMKI+GETF+I  P TA EVT+ +P + L +S+++K FG+R+K
Sbjct: 1   MGNSITVK----RKRAKVMKIDGETFRIKTPVTAREVTADYPGYVLLDSQAVKHFGVRSK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLP--------GRHRPLRRVCSDLHLSASDRLECLMISRR 133
           PL PN  LKPK  YFL++LP LP            P RRV S +H+ A +RL+ LM+SRR
Sbjct: 61  PLEPNQTLKPKKTYFLVELPKLPPETTAVDTENKLPYRRVMSGIHVGAKERLDMLMLSRR 120

Query: 134 SVSDLETLRFD-AQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEA-TERIVDLFA 193
           +VSD+   R D    F  + G      V+ RL R++  +LM+E  N   A  E+I+ ++ 
Sbjct: 121 TVSDVTIGRSDGGDGFGPELGPGH-TSVRLRLPRSQITKLMEENNNDASAIAEKILGIYM 180

[Close]

BLAST of Lsi05G003030 vs. TAIR10
Match: AT2G01340.1 (AT2G01340.1 Encodes a protein whose expression is responsive to nematode infection.)

HSP 1 Score: 125.6 bits (314), Expect = 4.2e-29
Identity = 80/175 (45.71%), Postives = 110/175 (62.86%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++G      +K TKVMKI+GETFK+  P TA EV    P H L +S+S+K +G RAK
Sbjct: 1   MGNSLG-----GKKTTKVMKIDGETFKLKTPVTAEEVLKDFPGHVLLDSESVKHYGARAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETL 133
           PL    +L+ K LYF+++    P +  P RRV S +H+SA +RLE LM++RRS SDL  L
Sbjct: 61  PLEAKQRLEAKRLYFVVE----PVKECPPRRVRSGIHVSAKERLESLMLARRSSSDLSIL 120

Query: 134 R-FDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLF-AKSR 187
           +         + GA R  +VK R+ +AE E+L+KE   + EAT++I  LF AK R
Sbjct: 121 KPPGGWTTEEEEGAVR--RVKVRIPKAELEKLVKEGATEAEATQKIAALFMAKQR 164

[Close]

BLAST of Lsi05G003030 vs. TAIR10
Match: AT5G37840.1 (AT5G37840.1 BEST Arabidopsis thaliana protein match is: plastid movement impaired 2 (TAIR:AT1G66480.1))

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-28
Identity = 73/185 (39.46%), Postives = 113/185 (61.08%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN + V    RR + KVMKI+G+ F++  P TA + T  +P   L +S+++K+ G+RAK
Sbjct: 1   MGNTIMV----RRNKVKVMKIDGDIFRLKTPVTASDATKEYPGFVLLDSETVKRLGVRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHR-PLRRVCS-DLHLSASDRLECLMISRRSVSDLE 133
           PL PN  LKP   YFL+ LP +  R++ P RRV S ++H+ A +RLE LM+SRR+VSD+ 
Sbjct: 61  PLEPNQILKPNHTYFLVDLPPVDKRNKLPYRRVMSGNIHVGAKERLEMLMLSRRTVSDVG 120

Query: 134 TLRFDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDG 193
             R D      + G TR   V+ RL R++  +LM+E  +  E   +I+  + +S     G
Sbjct: 121 AARSDVVGDGPELGHTR---VRLRLPRSQITKLMEESHDASEVAAKIISAYMESSGGIQG 178

Query: 194 GQEDE 197
           G++++
Sbjct: 181 GRDND 178

[Close]

BLAST of Lsi05G003030 vs. TAIR10
Match: AT1G71015.1 (AT1G71015.1 unknown protein)

HSP 1 Score: 102.4 bits (254), Expect = 3.8e-22
Identity = 61/168 (36.31%), Postives = 97/168 (57.74%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++G +     K   +M INGE+FK+  P  A  V    P H L ES+++K+ G+RAK
Sbjct: 1   MGNSLGTK-----KTATIMNINGESFKLKTPVEAGTVVKDFPGHVLLESEAVKRLGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETL 133
           PL P+  L+ K +YF+++LP    + R  RRV S + +SA +RLE L +SRRS SDL  +
Sbjct: 61  PLEPHQNLESKRIYFMVELPR-TWKERTPRRVRSGIQMSAKERLENLKLSRRSSSDLSVM 120

Query: 134 RFDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDL 182
           +   ++   +        VK +L + + E+L KE ++  + + +I  L
Sbjct: 121 K---KKTEVNDEEREVSSVKLKLPKWKIEKLRKESESGSDFSNKITQL 159

[Close]

BLAST of Lsi05G003030 vs. NCBI nr
Match: gi|700202755|gb|KGN57888.1| (hypothetical protein Csa_3G368690 [Cucumis sativus])

HSP 1 Score: 286.2 bits (731), Expect = 5.2e-74
Identity = 152/198 (76.77%), Postives = 169/198 (85.35%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRT-KVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRA 73
           MGNA+G RG RR+KRT KVMKINGET KIT+PTTAYEVTSH+P+H LYESKSLK FGLRA
Sbjct: 1   MGNAIG-RGSRRKKRTTKVMKINGETIKITLPTTAYEVTSHYPSHLLYESKSLKLFGLRA 60

Query: 74  KPLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLH-LSASDRLECLMISRRSVSDLE 133
           KPL+P+  L+PKTLYFLLQLPTLP  H  LRR CSDLH LSASDRLECL++SRRS+SDL+
Sbjct: 61  KPLDPHCHLEPKTLYFLLQLPTLPRDHCSLRRTCSDLHNLSASDRLECLLLSRRSLSDLQ 120

Query: 134 TLRFDAQRFSTDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDG 193
           TLRFD QR STD GA +PVQVKF+L RAEFERLMKECKNKVE T+RIVD  A+SRDN  G
Sbjct: 121 TLRFDPQRSSTDDGAAKPVQVKFQLPRAEFERLMKECKNKVEVTKRIVDYCARSRDNVYG 180

Query: 194 GQEDEFFWKMDDACSGKA 210
            Q+DE FWKM  A SGKA
Sbjct: 181 NQDDELFWKMGGAYSGKA 197

[Close]

BLAST of Lsi05G003030 vs. NCBI nr
Match: gi|449467677|ref|XP_004151549.1| (PREDICTED: uncharacterized protein At1g66480-like [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 3.9e-69
Identity = 138/179 (77.09%), Postives = 153/179 (85.47%), Query Frame = 1


Query: 32  MKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAKPLNPNYQLKPKTLYFLLQ 91
           MKINGET KIT+PTTAYEVTSH+P+H LYESKSLK FGLRAKPL+P+  L+PKTLYFLLQ
Sbjct: 1   MKINGETIKITLPTTAYEVTSHYPSHLLYESKSLKLFGLRAKPLDPHCHLEPKTLYFLLQ 60

Query: 92  LPTLPGRHRPLRRVCSDLH-LSASDRLECLMISRRSVSDLETLRFDAQRFSTDGGATRPV 151
           LPTLP  H  LRR CSDLH LSASDRLECL++SRRS+SDL+TLRFD QR STD GA +PV
Sbjct: 61  LPTLPRDHCSLRRTCSDLHNLSASDRLECLLLSRRSLSDLQTLRFDPQRSSTDDGAAKPV 120

Query: 152 QVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDGGQEDEFFWKMDDACSGKA 210
           QVKF+L RAEFERLMKECKNKVE T+RIVD  A+SRDN  G Q+DE FWKM  A SGKA
Sbjct: 121 QVKFQLPRAEFERLMKECKNKVEVTKRIVDYCARSRDNVYGNQDDELFWKMGGAYSGKA 179

[Close]

BLAST of Lsi05G003030 vs. NCBI nr
Match: gi|659114591|ref|XP_008457131.1| (PREDICTED: uncharacterized protein At1g66480-like [Cucumis melo])

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-61
Identity = 123/157 (78.34%), Postives = 136/157 (86.62%), Query Frame = 1


Query: 32  MKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAKPLNPNYQLKPKTLYFLLQ 91
           MKINGETFKIT+PTTAYEVTSH+PNH LYESKSL QFGLRAKPL+P+ QL+PKTLYFLLQ
Sbjct: 1   MKINGETFKITLPTTAYEVTSHYPNHLLYESKSLNQFGLRAKPLDPHCQLRPKTLYFLLQ 60

Query: 92  LPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETLRFDAQRFSTDGGATRPVQ 151
           LPTL G  R LRR CSDLHLSASDRLECL++SRRSVSDL+TLRFD QR     GA + VQ
Sbjct: 61  LPTLYGVDRSLRRTCSDLHLSASDRLECLLLSRRSVSDLQTLRFDPQRSLIHDGAAKSVQ 120

Query: 152 VKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDN 189
           VKF+L RAEFERLMKECKN VE T++IVD  A+SRDN
Sbjct: 121 VKFQLPRAEFERLMKECKNNVEVTKKIVDFCARSRDN 157

[Close]

BLAST of Lsi05G003030 vs. NCBI nr
Match: gi|802761901|ref|XP_012089911.1| (PREDICTED: uncharacterized protein At1g66480 [Jatropha curcas])

HSP 1 Score: 156.4 bits (394), Expect = 6.3e-35
Identity = 88/191 (46.07%), Postives = 123/191 (64.40%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN++G     R K+ K+MK++GETFK+  P  A +V   +P H L +S ++K FG+RAK
Sbjct: 1   MGNSIG-----RGKKAKIMKVDGETFKLKTPAKAMDVVKDYPGHVLLDSDAVKHFGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPL---RRVCSDLHLSASDRLECLMISRRSVSDL 133
           PL P  +L PK +YFL++LP LP    P+   RRV S +HLSA DRLE LM+SRRSVSDL
Sbjct: 61  PLEPEMELNPKKIYFLVELPKLPEEKEPISITRRVRSGIHLSAKDRLEFLMLSRRSVSDL 120

Query: 134 ETLRFDAQRFSTDG-GATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNG 193
             +R    +   DG   + PV+VK RL +A+ E+L++E K++VE  E+IVDLF +     
Sbjct: 121 SIVRPLPSQIPDDGHNGSGPVRVKMRLPKAQMEKLVEESKDEVEVAEKIVDLFME----- 180

Query: 194 DGGQEDEFFWK 201
           + G  +   WK
Sbjct: 181 NSGDRNRRQWK 181

[Close]

BLAST of Lsi05G003030 vs. NCBI nr
Match: gi|568857185|ref|XP_006482147.1| (PREDICTED: uncharacterized protein At1g66480 [Citrus sinensis])

HSP 1 Score: 153.7 bits (387), Expect = 4.1e-34
Identity = 85/181 (46.96%), Postives = 115/181 (63.54%), Query Frame = 1


Query: 14  MGNAMGVRGLRRRKRTKVMKINGETFKITIPTTAYEVTSHHPNHFLYESKSLKQFGLRAK 73
           MGN +G      R++ KVMKI+GET K+  P  A EV   +P + L +S+++K FG+RAK
Sbjct: 1   MGNTIG-----GRRKAKVMKIDGETIKLKTPIQASEVVKDYPGYVLLDSEAVKHFGIRAK 60

Query: 74  PLNPNYQLKPKTLYFLLQLPTLPGRHRPLRRVCSDLHLSASDRLECLMISRRSVSDLETL 133
           PL P  QLKPK +YFL++LP LP   +  RRV S + +SA DRLECLM+SRR+VSDL  +
Sbjct: 61  PLEPRQQLKPKKVYFLVELPKLPDEEKITRRVRSGIQMSAKDRLECLMLSRRAVSDLTAV 120

Query: 134 RFDAQRFS--TDGGATRPVQVKFRLSRAEFERLMKECKNKVEATERIVDLFAKSRDNGDG 193
              +   S    G  T  ++VK RL RA+ E+LM+E  N  E  E+IVDL+ +    G G
Sbjct: 121 NVRSSHGSEVARGPGTGQMRVKMRLPRAQVEKLMEESANDGEVAEKIVDLYVEKSGGGGG 176

The following BLAST results are available for this feature:

BLAST of Lsi05G003030 vs. Swiss-Prot


Match Name	E-value	Identity	Description
Y1648_ARATH	6.7e-29	42.11	Uncharacterized protein At1g66480 OS=Arabidopsis thaliana GN=At1g66480 PE=2 SV=1	[more]

BLAST of Lsi05G003030 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7W0_CUCSA	3.7e-74	76.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G368690 PE=4 SV=1	[more]
A0A067JIK5_JATCU	4.4e-35	46.07	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02495 PE=4 SV=1	[more]
V4UVZ8_9ROSI	8.2e-34	46.67	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012690mg PE=4 SV=1	[more]
A0A022QMX5_ERYGU	1.8e-33	49.43	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a023844mg PE=4 SV=1	[more]
A0A061EH62_THECC	2.4e-33	44.94	Plastid movement impaired 2 OS=Theobroma cacao GN=TCM_019256 PE=4 SV=1	[more]

BLAST of Lsi05G003030 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G66480.1	3.8e-30	42.11	plastid movement impaired 2	[more]
AT2G01340.1	4.2e-29	45.71	Encodes a protein whose expression is responsive to nematode infecti...	[more]
AT5G37840.1	3.5e-28	39.46	BEST Arabidopsis thaliana protein match is: plastid movement impaire...	[more]
AT1G71015.1	3.8e-22	36.31	unknown protein	[more]

BLAST of Lsi05G003030 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202755\|gb\|KGN57888.1\|	5.2e-74	76.77	hypothetical protein Csa_3G368690 [Cucumis sativus]	[more]
gi\|449467677\|ref\|XP_004151549.1\|	3.9e-69	77.09	PREDICTED: uncharacterized protein At1g66480-like [Cucumis sativus]	[more]
gi\|659114591\|ref\|XP_008457131.1\|	1.3e-61	78.34	PREDICTED: uncharacterized protein At1g66480-like [Cucumis melo]	[more]
gi\|802761901\|ref\|XP_012089911.1\|	6.3e-35	46.07	PREDICTED: uncharacterized protein At1g66480 [Jatropha curcas]	[more]
gi\|568857185\|ref\|XP_006482147.1\|	4.1e-34	46.96	PREDICTED: uncharacterized protein At1g66480 [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR025322	DUF4228_plant

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0009624	response to nematode
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003030.1	Lsi05G003030.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025322	Protein of unknown function DUF4228, plant	PFAM	PF14009	DUF4228	coord: 14..134 score: 1.3
None	No IPR available	unknown	Coil	Coil	coord: 158..178 scor
None	No IPR available	PANTHER	PTHR33148	FAMILY NOT NAMED	coord: 15..196 score: 3.2
None	No IPR available	PANTHER	PTHR33148:SF6	SUBFAMILY NOT NAMED	coord: 15..196 score: 3.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G003030	Cucsa.012710	Cucumber (Gy14) v1	cgylsiB322
Lsi05G003030	CmaCh01G003550	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G003030	CmoCh01G003710	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G003030	Cla013167	Watermelon (97103) v1	lsiwmB361
Lsi05G003030	ClCG05G010920	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G003030	Cla97C05G093220	Watermelon (97103) v2	lsiwmbB329
Lsi05G003030	Bhi01G001346	Wax gourd	lsiwgoB431
Lsi05G003030	Cp4.1LG02g16700	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G003030	CsGy3G021050	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G003030	Carg07442	Silver-seed gourd	carlsiB700
Lsi05G003030	CsaV3_3G021660	Cucumber (Chinese Long) v3	cuclsiB231

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G003030	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G003030	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G003030	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G003030	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G003030	Melon (DHL92) v3.6.1	lsimedB384
Lsi05G003030	Silver-seed gourd	carlsiB158