Cucsa.256690 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.256690
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRapid ALkalinization Factor
Locationscaffold02229 : 4242647 .. 4245430 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCCAACCAATAAAGAATCTCACATTTCAGAGCTTATATTGCTGGTGAAAGAAGACGAAACAGTGAAAAAACAAAAACAATTTCCCTTCAAAATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGATGATGATAATGATGATGGATGGATGGATGGAATGCCACTGGTAAGGTGAGAAATATAGTTGGTATTTAGTATTTACTTTGATTTTTAGAATGTGCCATAATGCTACATTTTGGGTTTTAATGACTGTTCTAATTTGGTGCATTGTTGAGTTTCTAGAGAGATCTGTGGAGAAAACCCTTGAGATTTGACTCTGCTCTCTGATTTTGGGAACTTGAGTCTTTGATTTCGTCTAACAATCCTAATTTAGCTAATTGGAACCTCATTACCGATTTCCTTCTCTTGGGTATTTAGTTGAAGAATGATTTTCTTCTCTGCTTTTCGGGTAATGGAGTTACTGGTTTATGAAGATTAAAGCGACAAGGGAAATGCTTAACTTGATTTGAAGTTGTGATAAAGAATGGGGTTGAGGAATGAAAACAGTGATCTTAGAATGTCAGATATTTTCATAGCTTTGATTCTTTTCGTGGTGTGAAGGAAGCTTAATTTATTACTTAAATAGATAACTTAAATTCGTTGGAAGAGGTAAGTATTATTTTTACTTTTTAGGATCTTGACTCTAATATCAACCAACAAATGATTTCCCCCTGGTTGTTTTTCTTCCCCTTGCACGTATGAACTTGCTTCATTTCTTTGCTGGAAATGGGGATCCATTGATATAAGTAGGATATGGAGATATGAATGTGGGATATGAGGGCGTGAATGTGTTGAAGTCAGCTGCCAATATAGAGAAGAACTAGTCATTTTGACAAAACTAAATCTATTGTGAGAAAGGAAAATATTCTATATTCTAATTCATGGAGTGTGTGTCAAACTAGCTAGTAAAGGGAAAACTGTTGTGACAGTTTCCTTCTTTTGAGACCATTCCCAACATCTATCATATCATCAATCGGAGGCAGTTCTTCCTCCTCCAAACCGCCCGTTTGCCAGATGAAAGTCGTTTGCCATACTTCGTTTTCATCATCATGTGCAACGAATGCATCTCCTCTATCATTTGTTTCATATTTTTTCCCAAAACAGGTAGCCATATTCTTACTCATTCCTCCAATTTCAGCCTCAGTTGCTCCCAAATGTACCTCTGTCCATTTCTGTGTCATCTTCTTCCACCTCTCCTGGATGCATGCTCTGCTACCCCACTTGTAAGGATACTTCTTACTATATTCTATAATTAACTTTGAAGAACATGATGACTAGGGAAAACTACCCAGCCGATTTGTGAGGGTTAGTAATCTCTTCCACAAGAACTATATTGAGGGTGTTTGGAGCATTGCCTATCCTAAAACTACAATAATTAGAGGCCTTAAACAGAGTATTTTTCATTGCAATGCTTAAGAAAATATAGGATTAAAGATTTATCTGACAAAGATAGTAACATTTTTAGTACACCACTGGAATGCAAGTAGAATGTTTGAACTTACGATCTATAATTCTATGAACTCAAGGTTCATTTGTTTTCACTATTTTCAATCATGAGCACTGTTTGGTCCTTTTAATATAGGTTTTTTGTATTTTGCCAGTGAAAATTGGTTTCTCACTTGTGCATTTTTTTTCACATTTCTTTTAAATGTCTAGTATAAAATGTTGAATTAAAACTAGAAACCATATGGTTATGCCCAAATTGCTCTTTACCATTTTACATACAAAATTTGAATTTCATTTCTCAACCCCAATTTTGGAAGGGTGGATTCTGAACTCTGTAAGTCACTTGTGGAGAAACGTATTGTTCTTACTAGAGTAGCTCTTAAACCTTCAGTGGTCATTATAACTGTGGTATAATGACCAAACCTTAAAATGTAGAAGAAATTTCATTAATTTCACAACATTGACTAAATTTGATATGAATCCTAAAGTATAGGTGAACTAAATTGTTGTTTATCAACATTTATAGACTAAACTATTACTTACATTTAAGTATATGGGGAGATCAAAACTGTAATTTAACCCTTTTTCAACTATCTATTGTTTATGCTTTACATTAACTCGTATATTTTTGCATCTTGATGATTGTTATTCTAAATGGTACTGCTCTCCAACATATCTAATTGATTCTGAGAATATAACTTTTGCAACTTGTTGGCAGCCGTTCAACTGTTGTGCCCAGAAGCAGATATCAACTACAGATTGTTGTTGTTCATTGAGATCGTCAAATCAAGCAAGAAAATTTATGTGCTTTTGTAAAAAGTGGTGTTGAATCTTGTTATGATAACTTGAACAGTTGTTGCTATTAGTAAATGGGAATTGCTTTTGAACACCAGACATTGTACCACAGAGCCTCTGGATCATAGAGAAGAATTTGACCATCTTGTTAAA

mRNA sequence

CATCCAACCAATAAAGAATCTCACATTTCAGAGCTTATATTGCTGGTGAAAGAAGACGAAACAGTGAAAAAACAAAAACAATTTCCCTTCAAAATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGATGATGATAATGATGATGGATGGATGGATGGAATGCCACTGGTAAGCCGTTCAACTGTTGTGCCCAGAAGCAGATATCAACTACAGATTGTTGTTGTTCATTGAGATCGTCAAATCAAGCAAGAAAATTTATGTGCTTTTGTAAAAAGTGGTGTTGAATCTTGTTATGATAACTTGAACAGTTGTTGCTATTAGTAAATGGGAATTGCTTTTGAACACCAGACATTGTACCACAGAGCCTCTGGATCATAGAGAAGAATTTGACCATCTTGTTAAA

Coding sequence (CDS)

ATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGA

Protein sequence

MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT*
Homology
[Close]
BLAST of Cucsa.256690 vs. Swiss-Prot
Match: RLF24_ARATH (Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 8.4e-26
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 1 

Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

[Close]
BLAST of Cucsa.256690 vs. Swiss-Prot
Match: RLF31_ARATH (Protein RALF-like 31 OS=Arabidopsis thaliana GN=RALFL31 PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 4.6e-24
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1 

Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

[Close]
BLAST of Cucsa.256690 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.1e-15
Identity = 42/82 (51.22%), Postives = 52/82 (63.41%), Query Frame = 1 

Query: 41  MEVMAKGVCNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSY 100
           M   + G C   IGEC+ + E   ++SE +RR+L   KKYISY  L+++ VPCSR G SY
Sbjct: 33  MPARSGGGCKGSIGECIAEEEEFELDSESNRRILAT-KKYISYGALQKNSVPCSRRGASY 92

Query: 101 YECHPG-PANSYDRGCEVITRC 119
           Y C PG  AN Y RGC  ITRC
Sbjct: 93  YNCKPGAQANPYSRGCSAITRC 113

[Close]
BLAST of Cucsa.256690 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.1e-15
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 1 

Query: 49  CNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ + E    +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]
BLAST of Cucsa.256690 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.3e-14
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 1 

Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
              K YISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  ATTK-YISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]
BLAST of Cucsa.256690 vs. TrEMBL
Match: A0A0A0L7V8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129640 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 2.8e-68
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 1 

Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]
BLAST of Cucsa.256690 vs. TrEMBL
Match: M5XU33_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013295mg PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 7.6e-34
Identity = 76/115 (66.09%), Postives = 87/115 (75.65%), Query Frame = 1 

Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESEISRRVL 
Sbjct: 17  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESEISRRVLA 76

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR  +DI T
Sbjct: 77  MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCARG-NDIKT 130

[Close]
BLAST of Cucsa.256690 vs. TrEMBL
Match: A0A067KSY9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01083 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-31
Identity = 65/107 (60.75%), Postives = 83/107 (77.57%), Query Frame = 1 

Query: 14  IFPLVFLQTHFSFCD--AILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVL 73
           I  L+FL THFS C+  ++  L S + +E++ +    C +KIG+CL + EMESEISRRVL
Sbjct: 8   ILALIFLYTHFSICNGVSVSGLNSLEITEIDAIVSRPCTRKIGDCLEEAEMESEISRRVL 67

Query: 74  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRC 119
           +MQKKYISY+TL+RDMVPC++PG SYY+CH G AN Y RGCEVITRC
Sbjct: 68  LMQKKYISYETLKRDMVPCAKPGASYYDCHAGEANPYSRGCEVITRC 114

[Close]
BLAST of Cucsa.256690 vs. TrEMBL
Match: B9GFJ2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32790g PE=4 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 6.0e-31
Identity = 67/112 (59.82%), Postives = 85/112 (75.89%), Query Frame = 1 

Query: 17  LVFLQTHFSFC--DAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVLMMQ 76
           L+FL T F+ C  D++  L S K SE++ +    C++KIGEC  + EMESEISRRVL+M 
Sbjct: 18  LLFLLTLFTICNADSVSGLNSLKTSEIDAIVSKGCSEKIGECFEESEMESEISRRVLLMH 77

Query: 77  KKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 127
           KKYISY+TLRRD+VPC +PG SYY+C+   A+ Y+RGCEVITRCAR V DIN
Sbjct: 78  KKYISYETLRRDLVPCDKPGASYYDCNARQAHPYNRGCEVITRCARSVKDIN 129

[Close]
BLAST of Cucsa.256690 vs. TrEMBL
Match: A0A061EJW1_THECC (RALFL33, putative OS=Theobroma cacao GN=TCM_020225 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.8e-30
Identity = 71/117 (60.68%), Postives = 88/117 (75.21%), Query Frame = 1 

Query: 14  IFPLVFLQTHFSFCDA--ILDLRS-FKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRV 73
           I  L FL   F  CDA  ILDL +  K SE++VM   VCN K+ +CL + EMESE +RRV
Sbjct: 10  ILSLSFL--FFQTCDAVSILDLNNALKHSEVDVMVPRVCNNKLEDCLEEQEMESESNRRV 69

Query: 74  LMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           L+MQ++YISY+TLRRDMVPC++PG SYY+C+ G AN Y+RGCEVITRCAR + DI T
Sbjct: 70  LVMQRRYISYETLRRDMVPCAKPGASYYDCNAGQANPYNRGCEVITRCARGIKDIKT 124

[Close]
BLAST of Cucsa.256690 vs. TAIR10
Match: AT3G23805.1 (AT3G23805.1 ralf-like 24)

HSP 1 Score: 117.9 bits (294), Expect = 4.7e-27
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 1 

Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

[Close]
BLAST of Cucsa.256690 vs. TAIR10
Match: AT4G13950.1 (AT4G13950.1 ralf-like 31)

HSP 1 Score: 112.1 bits (279), Expect = 2.6e-25
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1 

Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

[Close]
BLAST of Cucsa.256690 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 82.0 bits (201), Expect = 2.9e-16
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 1 

Query: 49  CNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ + E    +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]
BLAST of Cucsa.256690 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 79.3 bits (194), Expect = 1.9e-15
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 1 

Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
              K YISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  ATTK-YISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]
BLAST of Cucsa.256690 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 75.9 bits (185), Expect = 2.1e-14
Identity = 39/73 (53.42%), Postives = 48/73 (65.75%), Query Frame = 1 

Query: 49  CNQKIGECL--TDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PA 108
           C+  I EC+   + EM+SEI+RR+L   K YISY +L+R+ VPCSR G SYY C  G  A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 109 NSYDRGCEVITRC 119
           N Y RGC  I RC
Sbjct: 107 NPYSRGCSKIARC 118

[Close]
BLAST of Cucsa.256690 vs. NCBI nr
Match: gi|778677782|ref|XP_011650863.1| (PREDICTED: protein RALF-like 24 [Cucumis sativus])

HSP 1 Score: 265.8 bits (678), Expect = 4.0e-68
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 1 

Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]
BLAST of Cucsa.256690 vs. NCBI nr
Match: gi|659075762|ref|XP_008438316.1| (PREDICTED: protein RALF-like 24 [Cucumis melo])

HSP 1 Score: 260.0 bits (663), Expect = 2.2e-66
Identity = 126/128 (98.44%), Postives = 127/128 (99.22%), Query Frame = 1 

Query: 1   MAKSLFLF-FLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60
           MAKSLFLF FLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEM+VMAKGVCNQKIGECLTD
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDINT 128
           RDVHDINT
Sbjct: 121 RDVHDINT 128

[Close]
BLAST of Cucsa.256690 vs. NCBI nr
Match: gi|596217112|ref|XP_007223934.1| (hypothetical protein PRUPE_ppa013295mg [Prunus persica])

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-33
Identity = 76/115 (66.09%), Postives = 87/115 (75.65%), Query Frame = 1 

Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESEISRRVL 
Sbjct: 17  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESEISRRVLA 76

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR  +DI T
Sbjct: 77  MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCARG-NDIKT 130

[Close]
BLAST of Cucsa.256690 vs. NCBI nr
Match: gi|645232307|ref|XP_008222806.1| (PREDICTED: protein RALF-like 24 [Prunus mume])

HSP 1 Score: 148.3 bits (373), Expect = 9.3e-33
Identity = 72/108 (66.67%), Postives = 82/108 (75.93%), Query Frame = 1 

Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESE SRRVL 
Sbjct: 61  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESETSRRVLA 120

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 121
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR
Sbjct: 121 MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCAR 168

[Close]
BLAST of Cucsa.256690 vs. NCBI nr
Match: gi|502135175|ref|XP_004502245.1| (PREDICTED: protein RALF-like 24 [Cicer arietinum])

HSP 1 Score: 143.7 bits (361), Expect = 2.3e-31
Identity = 74/125 (59.20%), Postives = 85/125 (68.00%), Query Frame = 1 

Query: 7   LFFLFSIIFPLVFLQTHFSFCDAIL---DLRSFKGSEME-VMAKGVCNQKIGECLTDPEM 66
           LF   S+ F L    TH   C ++L   DL   K SE++ V+ K VC + IGECLT+PEM
Sbjct: 10  LFLFLSLTFFL--FHTHLPICTSLLSTVDLNLLKHSEIDTVITKRVCTKSIGECLTEPEM 69

Query: 67  ESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDV 126
           +SE +RRVL MQKKYISYDTL+RDMVPC R G SYY CHP  AN Y RGCEVIT CAR V
Sbjct: 70  DSETNRRVLAMQKKYISYDTLKRDMVPCDRAGASYYNCHPRQANPYSRGCEVITACARGV 129

Query: 127 HDINT 128
             I T
Sbjct: 130 QQIKT 132

The following BLAST results are available for this feature:
BLAST of Cucsa.256690 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RLF24_ARATH8.4e-2652.76Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1[more]
RLF31_ARATH4.6e-2462.79Protein RALF-like 31 OS=Arabidopsis thaliana GN=RALFL31 PE=3 SV=1[more]
RALF_TOBAC5.1e-1551.22Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1[more]
RLF22_ARATH5.1e-1555.26Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1[more]
RLF33_ARATH3.3e-1446.36Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1[more]
BLAST of Cucsa.256690 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7V8_CUCSA2.8e-68100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129640 PE=4 SV=1[more]
M5XU33_PRUPE7.6e-3466.09Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013295mg PE=4 SV=1[more]
A0A067KSY9_JATCU2.7e-3160.75Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01083 PE=4 SV=1[more]
B9GFJ2_POPTR6.0e-3159.82Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32790g PE=4 SV=2[more]
A0A061EJW1_THECC1.8e-3060.68RALFL33, putative OS=Theobroma cacao GN=TCM_020225 PE=4 SV=1[more]
BLAST of Cucsa.256690 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G23805.14.7e-2752.76 ralf-like 24[more]
AT4G13950.12.6e-2562.79 ralf-like 31[more]
AT3G05490.12.9e-1655.26 ralf-like 22[more]
AT4G15800.11.9e-1546.36 ralf-like 33[more]
AT1G02900.12.1e-1453.42 rapid alkalinization factor 1[more]
BLAST of Cucsa.256690 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778677782|ref|XP_011650863.1|4.0e-68100.00PREDICTED: protein RALF-like 24 [Cucumis sativus][more]
gi|659075762|ref|XP_008438316.1|2.2e-6698.44PREDICTED: protein RALF-like 24 [Cucumis melo][more]
gi|596217112|ref|XP_007223934.1|1.1e-3366.09hypothetical protein PRUPE_ppa013295mg [Prunus persica][more]
gi|645232307|ref|XP_008222806.1|9.3e-3366.67PREDICTED: protein RALF-like 24 [Prunus mume][more]
gi|502135175|ref|XP_004502245.1|2.3e-3159.20PREDICTED: protein RALF-like 24 [Cicer arietinum][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008801RALF
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.256690.1Cucsa.256690.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 61..120
score: 2.3
NoneNo IPR availablePANTHERPTHR33136FAMILY NOT NAMEDcoord: 1..122
score: 7.9
NoneNo IPR availablePANTHERPTHR33136:SF10PROTEIN RALF-LIKE 24-RELATEDcoord: 1..122
score: 7.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.256690Cla021396Watermelon (97103) v1cgywmB479
Cucsa.256690Csa3G129640Cucumber (Chinese Long) v2cgycuB366
Cucsa.256690MELO3C006289Melon (DHL92) v3.5.1cgymeB439
Cucsa.256690ClCG05G003080Watermelon (Charleston Gray)cgywcgB451
Cucsa.256690CSPI03G10560Wild cucumber (PI 183967)cgycpiB383
Cucsa.256690CmaCh14G019140Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.256690CmaCh06G011970Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.256690CmoCh06G012340Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.256690CmoCh14G019670Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.256690Lsi05G018650Bottle gourd (USVL1VR-Ls)cgylsiB415
Cucsa.256690Cp4.1LG08g02630Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.256690Cp4.1LG03g17800Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.256690CsaV3_3G010700Cucumber (Chinese Long) v3cgycucB387
Cucsa.256690Cla97C05G083710Watermelon (97103) v2cgywmbB452
Cucsa.256690Bhi01G000140Wax gourdcgywgoB523
Cucsa.256690Carg06309Silver-seed gourdcarcgyB1077
Cucsa.256690Carg14912Silver-seed gourdcarcgyB0693
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.256690Silver-seed gourdcarcgyB0269
Cucsa.256690Silver-seed gourdcarcgyB1190
Cucsa.256690Watermelon (97103) v2cgywmbB453
Cucsa.256690Cucurbita maxima (Rimu)cgycmaB0715
Cucsa.256690Cucurbita moschata (Rifu)cgycmoB0708
Cucsa.256690Cucurbita moschata (Rifu)cgycmoB0721
Cucsa.256690Watermelon (Charleston Gray)cgywcgB452
Cucsa.256690Cucurbita pepo (Zucchini)cgycpeB0670
Cucsa.256690Cucurbita pepo (Zucchini)cgycpeB0675
Cucsa.256690Bottle gourd (USVL1VR-Ls)cgylsiB412
Cucsa.256690Melon (DHL92) v3.6.1cgymedB432