CsaV3_3G010700 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_3G010700
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Rapid alkalinization factor
Location	chr3 : 8511713 .. 8514835 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTGGTTTTCATTATAAATTTATGGGTTTTAACATTGAAACAAATGCAAAAGCATTACTATTTATGAGCATCCAACCAATAAAGAATCTCACATTTCAGAGCTTATATTGCTGGTGAAAGAAGACGAAACAGTGAAAAAACAAAAACAATTTCCCTTCAAAATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGATGATGATAATGATGATGGATGGATGGATGGAATGCCACTGGTAAGGTGAGAAATATAGTTGGTATTTAGTATTTACTTTGATTTTTAGAATGTGCCATAATGCTACATTTTGGGTTTTAATGACTGTTCTAATTTGGTGCATTGTTGAGTTTCTAGAGAGATCTGTGGAGAAAACCCTTGAGATTTGACTCTGCTCTCTGATTTTGGGAACTTGAGTCTTTGATTTCGTCTAACAATCCTAATTTAGCTAATTGGAACCTCATTACCGATTTCCTTCTCTTGGGTATTTAGTTGAAGAATGATTTTCTTCTCTGCTTTTCGGGTAATGGAGTTACTGGTTTATGAAGATTAAAGCGACAAGGGAAATGCTTAACTTGATTTGAAGTTGTGATAAAGAATGGGGTTGAGGAATGAAAACAGTGATCTTAGAATGTCAGATATTTTCATAGCTTTGATTCTTTTCGTGGTGTGAAGGAAGCTTAATTTATTACTTAAATAGATAACTTAAATTCGTTGGAAGAGGTAAGTATTATTTTTACTTTTTAGGATCTTGACTCTAATATCAACCAACAAATGATTTCCCCCTGGTTGTTTTTCTTCCCCTTGCACGTATGAACTTGCTTCATTTCTTTGCTGGAAATGGGGATCCATTGATATAAGTAGGATATGGAGATATGAATGTGGGATATGAGGGCGTGAATGTGTTGAAGTCAGCTGCCAATATAGAGAAGAACTAGTCATTTTGACAAAACTAAATCTATTGTGAGAAAGGAAAATATTCTATATTCTAATTCATGGAGTGTGTGTCAAACTAGCTAGTAAAGGGAAAACTGTTGTGACAGTTTCCTTCTTTTGAGACCATTCCCAACATCTATCATATCATCAATCGGAGGCAGTTCTTCCTCCTCCAAACCGCCCGTTTGCCAGATGAAAGTCGTTTGCCATACTTCGTTTTCATCATCATGTGCAACGAATGCATCTCCTCTATCATTTGTTTCATATTTTTTCCCAAAACAGGTAGCCATATTCTTACTCATTCCTCCAATTTCAGCCTCAGTTGCTCCCAAATGTACCTCTGTCCATTTCTGTGTCATCTTCTTCCACCTCTCCTGGATGCATGCTCTGCTACCCCACTTGTAAGGATACTTCTTACTATATTCTATAATTAACTTTGAAGAACATGATGACTAGGGAAAACTACCCAGCCGATTTGTGAGGGTTAGTAATCTCTTCCACAAGAACTATATTGAGGGTGTTTGGAGCATTGCCTATCCTAAAACTACAATAATTAGAGGCCTTAAACAGAGTATTTTTCATTGCAATGCTTAAGAAAATATAGGATTAAAGATTTATCTGACAAAGATAGTAACATTTTTAGTACACCACTGGAATGCAAGTAGAATGTTTGAACTTACGATCTATAATTCTATGAACTCAAGGTTCATTTGTTTTCACTATTTTCAATCATGAGCACTGTTTGGTCCTTTTAATATAGGTTTTTTGTATTTTGCCAGTGAAAATTGGTTTCTCACTTGTGCATTTTTTTTCACATTTCTTTTAAATGTCTAGTATAAAATGTTGAATTAAAACTAGAAACCATATGGTTATGCCCAAATTGCTCTTTACCATTTTACATACAAAATTTGAATTTCATTTCTCAACCCCAATTTTGGAAGGGTGGATTCTGAACTCTGTAAGTCACTTGTGGAGAAACGTATTGTTCTTACTAGAGTAGCTCTTAAACCTTCAGTGGTCATTATAACTGTGGTATAATGACCAAACCTTAAAATGTAGAAGAAATTTCATTAATTTCACAACATTGACTAAATTTGATATGAATCCTAAAGTATAGGTGAACTAAATTGTTGTTTATCAACATTTATAGACTAAACTATTACTTACATTTAAGTATATGGGGAGATCAAAACTGTAATTTAACCCTTTTTCAACTATCTATTGTTTATGCTTTACATTAACTCGTATATTTTTGCATCTTGATGATTGTTATTCTAAATGGTACTGCTCTCCAACATATCTAATTGATTCTGAGAATATAACTTTTGCAACTTGTTGGCAGCCGTTCAACTGTTGTGCCCAGAAGCAGATATCAACTACAGATTGTTGTTGTTCATTGAGATCGTCAAATCAAGCAAGAAAATTTATGTGCTTTTGTAAAAAGTGGTGTTGAATCTTGTTATGATAACTTGAACAGTTGTTGCTATTAGTAAATGGGAATTGCTTTTGAACACCAGACATTGTACCACAGAGCCTCTGGATCATAGAGAAGAATTTGACCATCTTGTTAAATTCACAAATCATAGCCTCGGCTTGGCTTACCTCTCTTTGGTTCTGTTGCTTCTGCCAGCACATACGAATTTTGTTTTTCATGCAAGTAATAATTTAGACTTTGCATTTTAGTTTATCATGAATTAGTCAAATGTAGTTTAAGTATAAAGACTAGAATTAGCTAAATTTGATGATATTTTTGAAGAACCCACCTCAAAAAGTTGGCTATTGGGGTGGGAGAGAGCCAATTTTCTCCAAAACGTCAACAACTAGGATGGAAAATCAAGCCC

mRNA sequence

ATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGA

Coding sequence (CDS)

ATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGA

Protein sequence

MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT

Homology

[Close]

BLAST of CsaV3_3G010700 vs. NCBI nr
Match: XP_011650863.1 (PREDICTED: protein RALF-like 24 [Cucumis sativus] >XP_011650864.1 PREDICTED: protein RALF-like 24 [Cucumis sativus] >KGN56707.1 hypothetical protein Csa_3G129640 [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 6.5e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]

BLAST of CsaV3_3G010700 vs. NCBI nr
Match: XP_008438316.1 (PREDICTED: protein RALF-like 24 isoform X1 [Cucumis melo] >XP_008438319.1 PREDICTED: protein RALF-like 24 isoform X1 [Cucumis melo])

HSP 1 Score: 256.9 bits (655), Expect = 3.6e-65
Identity = 126/128 (98.44%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MAKSLFL-FFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60
           MAKSLFL FFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEM+VMAKGVCNQKIGECLTD
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDINT 128
           RDVHDINT
Sbjct: 121 RDVHDINT 128

[Close]

BLAST of CsaV3_3G010700 vs. NCBI nr
Match: XP_023526933.1 (protein RALF-like 24 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242.7 bits (618), Expect = 7.0e-61
Identity = 114/121 (94.21%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 7   LFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEI 66
           LFFLFSI+ PLVFLQTHFSFCDAILDLRS KGSE++VMAKGVCNQKIGECLT+PEMESE+
Sbjct: 32  LFFLFSILLPLVFLQTHFSFCDAILDLRSLKGSEIDVMAKGVCNQKIGECLTEPEMESEV 91

Query: 67  SRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 126
           SRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN
Sbjct: 92  SRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 151

Query: 127 T 128
           T
Sbjct: 152 T 152

[Close]

BLAST of CsaV3_3G010700 vs. NCBI nr
Match: XP_022974738.1 (protein RALF-like 24 [Cucurbita maxima])

HSP 1 Score: 228.8 bits (582), Expect = 1.0e-56
Identity = 104/121 (85.95%), Postives = 116/121 (95.87%), Query Frame = 0


Query: 7   LFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEI 66
           LFFL SI+ PL+FLQTHF FCDA+LDLRS KG+++++M KGVCNQKIGECLT+P+MESEI
Sbjct: 6   LFFLLSILLPLIFLQTHFPFCDAVLDLRSLKGNQIDLMPKGVCNQKIGECLTEPQMESEI 65

Query: 67  SRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 126
           SRRVLMMQK+YISY+TLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN
Sbjct: 66  SRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 125

Query: 127 T 128
           T
Sbjct: 126 T 126

[Close]

BLAST of CsaV3_3G010700 vs. NCBI nr
Match: XP_023539976.1 (protein RALF-like 31 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.0 bits (567), Expect = 5.7e-55
Identity = 107/128 (83.59%), Postives = 117/128 (91.41%), Query Frame = 0


Query: 1   MAKSLFLFFLFSI-IFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60
           MAK   LFFL SI + PL FLQT F FCDA+LDLRS KG+++++M KGVCNQKIGECLT+
Sbjct: 1   MAKPTTLFFLLSILLLPLTFLQTLFPFCDAVLDLRSLKGNQIDLMPKGVCNQKIGECLTE 60

Query: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQK+YISY+TLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKRYISYETLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDINT 128
           RDVHDINT
Sbjct: 121 RDVHDINT 128

[Close]

BLAST of CsaV3_3G010700 vs. TAIR10
Match: AT3G23805.1 (ralf-like 24)

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

[Close]

BLAST of CsaV3_3G010700 vs. TAIR10
Match: AT4G13950.1 (ralf-like 31)

HSP 1 Score: 114.4 bits (285), Expect = 5.2e-26
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

[Close]

BLAST of CsaV3_3G010700 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 84.0 bits (206), Expect = 7.6e-17
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 0


Query: 49  CNQKIGECLTDP---EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ +    E +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]

BLAST of CsaV3_3G010700 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-15
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 0


Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
               KYISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  -ATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of CsaV3_3G010700 vs. TAIR10
Match: AT1G02900.1 (rapid alkalinization factor 1)

HSP 1 Score: 77.4 bits (189), Expect = 7.1e-15
Identity = 39/73 (53.42%), Postives = 48/73 (65.75%), Query Frame = 0


Query: 49  CNQKIGECL--TDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PA 108
           C+  I EC+   + EM+SEI+RR+L    KYISY +L+R+ VPCSR G SYY C  G  A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRIL-ATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 109 NSYDRGCEVITRC 119
           N Y RGC  I RC
Sbjct: 107 NPYSRGCSKIARC 118

[Close]

BLAST of CsaV3_3G010700 vs. Swiss-Prot
Match: sp|Q9LK37|RLF24_ARATH (Protein RALF-like 24 OS=Arabidopsis thaliana OX=3702 GN=RALFL24 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-25
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

[Close]

BLAST of CsaV3_3G010700 vs. Swiss-Prot
Match: sp|Q2HIM9|RLF31_ARATH (Protein RALF-like 31 OS=Arabidopsis thaliana OX=3702 GN=RALFL31 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 9.4e-25
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

[Close]

BLAST of CsaV3_3G010700 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-15
Identity = 42/82 (51.22%), Postives = 52/82 (63.41%), Query Frame = 0


Query: 41  MEVMAKGVCNQKIGECLTDP---EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSY 100
           M   + G C   IGEC+ +    E++SE +RR+L   KKYISY  L+++ VPCSR G SY
Sbjct: 33  MPARSGGGCKGSIGECIAEEEEFELDSESNRRIL-ATKKYISYGALQKNSVPCSRRGASY 92

Query: 101 YECHPG-PANSYDRGCEVITRC 119
           Y C PG  AN Y RGC  ITRC
Sbjct: 93  YNCKPGAQANPYSRGCSAITRC 113

[Close]

BLAST of CsaV3_3G010700 vs. Swiss-Prot
Match: sp|Q9MA62|RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-15
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 0


Query: 49  CNQKIGECLTDP---EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ +    E +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRIL-AQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]

BLAST of CsaV3_3G010700 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-14
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 0


Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
               KYISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  -ATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of CsaV3_3G010700 vs. TrEMBL
Match: tr|A0A0A0L7V8|A0A0A0L7V8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G129640 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.3e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]

BLAST of CsaV3_3G010700 vs. TrEMBL
Match: tr|A0A1S3AWQ1|A0A1S3AWQ1_CUCME (protein RALF-like 24 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.4e-65
Identity = 126/128 (98.44%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MAKSLFL-FFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60
           MAKSLFL FFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEM+VMAKGVCNQKIGECLTD
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDINT 128
           RDVHDINT
Sbjct: 121 RDVHDINT 128

[Close]

BLAST of CsaV3_3G010700 vs. TrEMBL
Match: tr|A0A1S4DSF8|A0A1S4DSF8_CUCME (protein RALF-like 31 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.1e-49
Identity = 93/94 (98.94%), Postives = 94/94 (100.00%), Query Frame = 0


Query: 34  RSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRP 93
           RSFKGSEM+VMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRP
Sbjct: 24  RSFKGSEMDVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRP 83

Query: 94  GVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           GVSYYECHPGPANSYDRGCEVITRCARDVHDINT
Sbjct: 84  GVSYYECHPGPANSYDRGCEVITRCARDVHDINT 117

[Close]

BLAST of CsaV3_3G010700 vs. TrEMBL
Match: tr|M5XU33|M5XU33_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G165400 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.3e-34
Identity = 76/115 (66.09%), Postives = 87/115 (75.65%), Query Frame = 0


Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESEISRRVL 
Sbjct: 17  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESEISRRVLA 76

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR  +DI T
Sbjct: 77  MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCARG-NDIKT 130

[Close]

BLAST of CsaV3_3G010700 vs. TrEMBL
Match: tr|A0A251LAM0|A0A251LAM0_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_03G143800 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.6e-32
Identity = 67/107 (62.62%), Postives = 83/107 (77.57%), Query Frame = 0


Query: 14  IFPLVFLQTHFSFCD--AILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVL 73
           +  L  L TH  FCD  ++  L SF+ SE++ M +  C +KIG+CL +PEMESEIS+RVL
Sbjct: 38  LLALFLLYTHLKFCDGVSVSGLNSFEKSEIDAMVRRGCTKKIGDCLEEPEMESEISKRVL 97

Query: 74  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRC 119
           MMQKKYISY+TL+RDMVPC++PG SYY+CH G AN Y RGCEVITRC
Sbjct: 98  MMQKKYISYETLKRDMVPCAKPGASYYDCHAGEANPYSRGCEVITRC 144

The following BLAST results are available for this feature:

BLAST of CsaV3_3G010700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650863.1	6.5e-67	100.00	PREDICTED: protein RALF-like 24 [Cucumis sativus] >XP_011650864.1 PREDICTED: pro...	[more]
XP_008438316.1	3.6e-65	98.44	PREDICTED: protein RALF-like 24 isoform X1 [Cucumis melo] >XP_008438319.1 PREDIC...	[more]
XP_023526933.1	7.0e-61	94.21	protein RALF-like 24 [Cucurbita pepo subsp. pepo]	[more]
XP_022974738.1	1.0e-56	85.95	protein RALF-like 24 [Cucurbita maxima]	[more]
XP_023539976.1	5.7e-55	83.59	protein RALF-like 31 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsaV3_3G010700 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G23805.1	1.1e-26	52.76	ralf-like 24	[more]
AT4G13950.1	5.2e-26	62.79	ralf-like 31	[more]
AT3G05490.1	7.6e-17	55.26	ralf-like 22	[more]
AT4G15800.1	1.1e-15	46.36	ralf-like 33	[more]
AT1G02900.1	7.1e-15	53.42	rapid alkalinization factor 1	[more]

BLAST of CsaV3_3G010700 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LK37\|RLF24_ARATH	1.9e-25	52.76	Protein RALF-like 24 OS=Arabidopsis thaliana OX=3702 GN=RALFL24 PE=3 SV=1	[more]
sp\|Q2HIM9\|RLF31_ARATH	9.4e-25	62.79	Protein RALF-like 31 OS=Arabidopsis thaliana OX=3702 GN=RALFL31 PE=3 SV=1	[more]
sp\|Q945T0\|RALF_TOBAC	1.4e-15	51.22	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
sp\|Q9MA62\|RLF22_ARATH	1.4e-15	55.26	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]
sp\|Q8L9P8\|RLF33_ARATH	2.0e-14	46.36	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]

BLAST of CsaV3_3G010700 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L7V8\|A0A0A0L7V8_CUCSA	4.3e-67	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G129640 PE=4 SV=1	[more]
tr\|A0A1S3AWQ1\|A0A1S3AWQ1_CUCME	2.4e-65	98.44	protein RALF-like 24 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=4 SV=...	[more]
tr\|A0A1S4DSF8\|A0A1S4DSF8_CUCME	4.1e-49	98.94	protein RALF-like 31 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483460 PE=4 SV=...	[more]
tr\|M5XU33\|M5XU33_PRUPE	6.3e-34	66.09	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G165400 PE=4 SV=1	[more]
tr\|A0A251LAM0\|A0A251LAM0_MANES	1.6e-32	62.62	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_03G143800 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G010700.1	CsaV3_3G010700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 61..120 e-value: 2.4E-28 score: 98.7
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 5..126
None	No IPR available	PANTHER	PTHR33136:SF10	PROTEIN RALF-LIKE 24-RELATED	coord: 5..126

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_3G010700	CSPI03G10560	Wild cucumber (PI 183967)	cpicucB144
CsaV3_3G010700	Cucsa.256690	Cucumber (Gy14) v1	cgycucB387
CsaV3_3G010700	CmaCh06G011970	Cucurbita maxima (Rimu)	cmacucB0965
CsaV3_3G010700	CmoCh14G019670	Cucurbita moschata (Rifu)	cmocucB0283
CsaV3_3G010700	CmoCh06G012340	Cucurbita moschata (Rifu)	cmocucB0953
CsaV3_3G010700	Cp4.1LG08g02630	Cucurbita pepo (Zucchini)	cpecucB1056
CsaV3_3G010700	Cp4.1LG03g17800	Cucurbita pepo (Zucchini)	cpecucB0759
CsaV3_3G010700	Carg14912	Silver-seed gourd	carcucB0652
CsaV3_3G010700	Carg06309	Silver-seed gourd	carcucB1003
CsaV3_3G010700	Cla021396	Watermelon (97103) v1	cucwmB239
CsaV3_3G010700	MELO3C006289	Melon (DHL92) v3.5.1	cucmeB236
CsaV3_3G010700	ClCG05G003080	Watermelon (Charleston Gray)	cucwcgB225
CsaV3_3G010700	Lsi05G018650	Bottle gourd (USVL1VR-Ls)	cuclsiB228
CsaV3_3G010700	Cla97C05G083710	Watermelon (97103) v2	cucwmbB206
CsaV3_3G010700	Bhi01G000140	Wax gourd	cucwgoB273

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_3G010700	Silver-seed gourd	carcucB0260
CsaV3_3G010700	Silver-seed gourd	carcucB1126
CsaV3_3G010700	Cucumber (Gy14) v2	cgybcucB117
CsaV3_3G010700	Cucurbita maxima (Rimu)	cmacucB0291
CsaV3_3G010700	Cucurbita maxima (Rimu)	cmacucB0425
CsaV3_3G010700	Cucurbita moschata (Rifu)	cmocucB0420
CsaV3_3G010700	Cucurbita moschata (Rifu)	cmocucB1032
CsaV3_3G010700	Cucurbita pepo (Zucchini)	cpecucB0153
CsaV3_3G010700	Cucurbita pepo (Zucchini)	cpecucB0385
CsaV3_3G010700	Wild cucumber (PI 183967)	cpicucB093
CsaV3_3G010700	Bottle gourd (USVL1VR-Ls)	cuclsiB163
CsaV3_3G010700	Melon (DHL92) v3.6.1	cucmedB230
CsaV3_3G010700	Watermelon (Charleston Gray)	cucwcgB254
CsaV3_3G010700	Watermelon (97103) v2	cucwmbB233