CSPI03G10560 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G10560
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Rapid ALkalinization Factor
Location	Chr3 : 8397471 .. 8400343 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATGCAAAAGCATTACTATTTATGAGCATCCAACCAATAAAGAATCTCACATTTCAGAGCTTATATTGCTGGTGAAAGAAGACGAAACAGTGAAAAAACAAAAACAATTTCCCTTCAAAATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGATGATGATAATAATGATGGATGGATGGATGGAATGCCACTGGTAAGGTGAGAAATATAGTTGGTATTTAGTATTTACTTTGATTTTTAGAATGTGCCATAATGCTACATTTTGGGTTTTAATGACTGTTCTAATTTGGTGCATTGTTGAGTTTCTAGAGAGATCTGTGGAGAAAACCCTTGAGATTTGACTCTGCTCTCTGATTTTGGGAACTCGAGTCTTTGATTTCGTCTAACAATCCTAATTTAGCTAATTGGAACTTCATTACCGATTTCCTTCTCTTGGGTATTTAGTTGAAGAATGATTTTCTTCTCTGCTTTTCGGGTAATGGAGTTACTGGTTTATGAAGATTAAAGCGACAAGGGAAATGCTTAACTTGATTTGAAGTTGTGATAAAGAATGGGGTTGAGGAATGAAAACAGTGATCTTAGAATGTCAGATATTTTCATAGCTTTGATTCTTTTCGTGGTGTGAAGGAAGCTTAATTTATTACTTAAATAGATAACTTAAATTCGTTGGAAGAGGTAAGTATTATTTTTACTTTTTAGGATCTTGACTCTAATATCAACCAACAAATGATTTCCCCCTGGTTGTTTTTCTTCCCCTTGCACGTATGAACTTGCTTCATTTCTTTGCTGGAAATGGGGATCCATTGATATAAGTAGGATATGAAGATATGAATGTGGGATATGGGGGCGTGAATGTGTTGAAGTCAGCTTGCCAATATAGAGAAGAACTAGTCATTTTGACAAAACTAAATCTATTGTGAGAAAGGAAAATATTCTATATTCTAATTCATGGAGTGTGTGTCAAACTAGCTAGTAAAGGGAAAACTGTTGTGACAGTTTCCTTCTTTTGAGACCATTCCCAACATCTATCATATCATCAATCGGAGGCAGTTCTTCCTCCTCCAAACCGCCCGTTTGCCAGATGAAAGTCGTTTGCCATACTTCGTTTTCATCATCATGTGCAACGAATGCATCTCCTCTATCATTTGTTTCATATTTTTTCCCAAAACAGGTAGCCATATTCTTACTCATTCCTCCAATTTCAGCCTCAGTTGCTCCCAAATGTACCTCTGTCCATTTCTGCGTCATCTTCTTCCACCTCTCCTGGATGCATGCTCTGCTACCCCACTTGTAAGGCTACTTCTTACTATATTCTATAATTAACTTTGAAGAACATGATGACTAGGGAAAACTACCCAGCCGATTTGTGAGGGTTAGTAATCTCTTCCACAAGAACTATATTGAGGGTGTTTGGAGCATTGCCTATACTAAAACTACAATAATTAGAGGCCTTAAACAGAGTCTTTTTCATTGCAATGCTTAAGAAAATATAGGATTAAAGATTTATCTGACAAAGATAGTAACATTTTTAGTACACCACTAGAATGCAAGTAGAATGTTTGAACTTACGATCTATAATTCTATGAACTCAAGGTTCATTTGTTTTCACTATTTTCAATCATGGGCACTGTTTGATCCTTTTAATATAGGTTTTTTGTATTTTGCCAGTGAAAATTGGTTTCTCACTTGTGCATTTTTTTTCACATTTCTTTTAAATGTCTAGTATAAAATGTTGAATTAAAACTAGAAACCATATGGTTATGCCCAAATTGCTCTTTACCATTTTACATACAAAATTTGAATTTCATTTCTCAACCCCAATTTTGGAAGGGTGGATTCTGAACTCTGTAAGTCACTTGTGGAGAAACGTATTGTTCTTACTAGAGTAGCTCTTAAACCTTCAGTGGTCATTATAACTGTGGTATAATGACCAAACCTTAAAATGTAGAAGAAATTTCATTAATTTCACAACATTGACTAAATTTGATATGAATCCTAAAGTATAGGTGAACTAAATTGTTGTTTATCAACATTTATAGACTAAACTATTACTTACATTTAAGTATATGGGGAGATCAAAACTGTAATTTAACCCTTTTTCAACTATCTATTGTTTATGCTTTACATTAACTCGTATATTTTTGCATCTTGATGATTGTTATTCTAAATGGTACTGCTCTCCAACATATCTAATTGATTCTGAGAATATAACTTTTGCAACTTGTTGGCAGCCGTTCAACTGTTGTGCCCAGAAGCAGATATCAACTACAGATTGTTGTTGTTCATTGAGATCGTCAAATCAAGCAAGAAAATTTATGTGCTTTTGTAAAAAGTGGTGTTGAATCTTGTTATGATAACTTGAACAGTTGTTGCTATTAGTAAATGGGAATTGCTTTTGAACACCAGACATTGTACCACAGAGCCTCTGGATCATAGAGAAGAATTTGACCATCTTGTTAAATTCACAAATCATAGCCTCGGCTTGGCTTACCTCTCTTTGGTTCTGTTGCTTCTGCCAGCAC

mRNA sequence

ATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGA

Coding sequence (CDS)

ATGGCAAAATCCCTTTTCCTCTTCTTCCTCTTTTCCATCATTTTCCCACTTGTTTTCCTTCAAACCCATTTCTCATTTTGCGATGCGATTCTTGATCTCAGATCTTTTAAGGGTAGTGAAATGGAGGTAATGGCAAAAGGGGTTTGCAATCAGAAGATCGGTGAGTGTTTGACTGACCCAGAAATGGAATCAGAAATCAGTAGAAGAGTTCTGATGATGCAGAAGAAGTATATAAGCTATGATACACTTAGAAGGGACATGGTTCCTTGTTCAAGACCAGGTGTTTCGTATTATGAATGTCATCCTGGTCCAGCAAATTCTTATGACAGAGGCTGTGAAGTCATTACTAGATGTGCCAGAGATGTTCATGACATAAACACTTGA

Homology

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BLAST of CSPI03G10560 vs. Swiss-Prot
Match: RLF24_ARATH (Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-25
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

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BLAST of CSPI03G10560 vs. Swiss-Prot
Match: RLF31_ARATH (Protein RALF-like 31 OS=Arabidopsis thaliana GN=RALFL31 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 9.3e-25
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

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BLAST of CSPI03G10560 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.3e-15
Identity = 42/82 (51.22%), Postives = 52/82 (63.41%), Query Frame = 1


Query: 41  MEVMAKGVCNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSY 100
           M   + G C   IGEC+ + E   ++SE +RR+L   KKYISY  L+++ VPCSR G SY
Sbjct: 33  MPARSGGGCKGSIGECIAEEEEFELDSESNRRILAT-KKYISYGALQKNSVPCSRRGASY 92

Query: 101 YECHPG-PANSYDRGCEVITRC 119
           Y C PG  AN Y RGC  ITRC
Sbjct: 93  YNCKPGAQANPYSRGCSAITRC 113

[Close]

BLAST of CSPI03G10560 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.8e-15
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 1


Query: 49  CNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ + E    +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]

BLAST of CSPI03G10560 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.5e-14
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 1


Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
              K YISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  ATTK-YISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of CSPI03G10560 vs. TrEMBL
Match: A0A0A0L7V8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129640 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 2.3e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 1


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]

BLAST of CSPI03G10560 vs. TrEMBL
Match: M5XU33_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013295mg PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 3.4e-34
Identity = 76/115 (66.09%), Postives = 87/115 (75.65%), Query Frame = 1


Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESEISRRVL 
Sbjct: 17  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESEISRRVLA 76

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR  +DI T
Sbjct: 77  MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCARG-NDIKT 130

[Close]

BLAST of CSPI03G10560 vs. TrEMBL
Match: B9GFJ2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32790g PE=4 SV=2)

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-31
Identity = 67/112 (59.82%), Postives = 85/112 (75.89%), Query Frame = 1


Query: 17  LVFLQTHFSFC--DAILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVLMMQ 76
           L+FL T F+ C  D++  L S K SE++ +    C++KIGEC  + EMESEISRRVL+M 
Sbjct: 18  LLFLLTLFTICNADSVSGLNSLKTSEIDAIVSKGCSEKIGECFEESEMESEISRRVLLMH 77

Query: 77  KKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 127
           KKYISY+TLRRD+VPC +PG SYY+C+   A+ Y+RGCEVITRCAR V DIN
Sbjct: 78  KKYISYETLRRDLVPCDKPGASYYDCNARQAHPYNRGCEVITRCARSVKDIN 129

[Close]

BLAST of CSPI03G10560 vs. TrEMBL
Match: A0A067KSY9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01083 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-31
Identity = 65/107 (60.75%), Postives = 83/107 (77.57%), Query Frame = 1


Query: 14  IFPLVFLQTHFSFCD--AILDLRSFKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRVL 73
           I  L+FL THFS C+  ++  L S + +E++ +    C +KIG+CL + EMESEISRRVL
Sbjct: 8   ILALIFLYTHFSICNGVSVSGLNSLEITEIDAIVSRPCTRKIGDCLEEAEMESEISRRVL 67

Query: 74  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRC 119
           +MQKKYISY+TL+RDMVPC++PG SYY+CH G AN Y RGCEVITRC
Sbjct: 68  LMQKKYISYETLKRDMVPCAKPGASYYDCHAGEANPYSRGCEVITRC 114

[Close]

BLAST of CSPI03G10560 vs. TrEMBL
Match: A0A061EJW1_THECC (RALFL33, putative OS=Theobroma cacao GN=TCM_020225 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.3e-30
Identity = 71/117 (60.68%), Postives = 88/117 (75.21%), Query Frame = 1


Query: 14  IFPLVFLQTHFSFCDA--ILDLRS-FKGSEMEVMAKGVCNQKIGECLTDPEMESEISRRV 73
           I  L FL   F  CDA  ILDL +  K SE++VM   VCN K+ +CL + EMESE +RRV
Sbjct: 10  ILSLSFL--FFQTCDAVSILDLNNALKHSEVDVMVPRVCNNKLEDCLEEQEMESESNRRV 69

Query: 74  LMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           L+MQ++YISY+TLRRDMVPC++PG SYY+C+ G AN Y+RGCEVITRCAR + DI T
Sbjct: 70  LVMQRRYISYETLRRDMVPCAKPGASYYDCNAGQANPYNRGCEVITRCARGIKDIKT 124

[Close]

BLAST of CSPI03G10560 vs. TAIR10
Match: AT3G23805.1 (AT3G23805.1 ralf-like 24)

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 67/127 (52.76%), Postives = 84/127 (66.14%), Query Frame = 1


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           M++SL L +L      L+ LQTH S     + + S  G E++ M     N  IGE   + 
Sbjct: 1   MSRSLALVYL-----SLLCLQTHLSI-SVTVPIPSVNG-EIDAMLNR--NGVIGEEEGEE 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
            M SEISRRV+MM+K+YISY+TLRRDMVPC +PG SYY C  G AN+Y+RGC VITRCAR
Sbjct: 61  MMPSEISRRVMMMRKQYISYETLRRDMVPCQKPGASYYACRSGQANAYNRGCSVITRCAR 118

Query: 121 DVHDINT 128
           D +DI T
Sbjct: 121 DTNDIKT 118

[Close]

BLAST of CSPI03G10560 vs. TAIR10
Match: AT4G13950.1 (AT4G13950.1 ralf-like 31)

HSP 1 Score: 114.4 bits (285), Expect = 5.2e-26
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 42  EVMAKGVCNQKIGECLTDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECH 101
           E+ A  + N  IGE   +  M +EISRRVLM QK+YI Y+TLRRDMVPC +PG SYY+C 
Sbjct: 30  EIDAMLIRNSIIGE--DEDLMPTEISRRVLMAQKRYIGYETLRRDMVPCQKPGASYYDCR 89

Query: 102 PGPANSYDRGCEVITRCARDVHDINT 128
            G ANSY RGC+ ITRCARD +DINT
Sbjct: 90  SGQANSYSRGCDTITRCARDTNDINT 113

[Close]

BLAST of CSPI03G10560 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 83.6 bits (205), Expect = 9.9e-17
Identity = 42/76 (55.26%), Postives = 49/76 (64.47%), Query Frame = 1


Query: 49  CNQKIGECLTDPE---MESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-P 108
           C   I EC+ + E    +S+ISRR+L  QKKYISY  +RR+ VPCSR G SYY C  G  
Sbjct: 45  CTGSIAECIAEEEEMEFDSDISRRILA-QKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 109 ANSYDRGCEVITRCAR 121
           AN Y RGC  ITRC R
Sbjct: 105 ANPYSRGCSTITRCRR 119

[Close]

BLAST of CSPI03G10560 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 79.7 bits (195), Expect = 1.4e-15
Identity = 51/110 (46.36%), Postives = 60/110 (54.55%), Query Frame = 1


Query: 17  LVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP-----EMESEISRRVL 76
           +  L  HF F  A+    S  G  + + +K  CN  I EC         EM+SEI+RR+L
Sbjct: 13  IAILTVHFLFA-AVTSQSS--GDFVPIESK--CNGTIAECSLSTAEEEFEMDSEINRRIL 72

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PANSYDRGCEVITRCAR 121
              K YISY  LRR+ VPCSR G SYY C  G  AN Y RGC  ITRC R
Sbjct: 73  ATTK-YISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of CSPI03G10560 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 77.4 bits (189), Expect = 7.1e-15
Identity = 39/73 (53.42%), Postives = 48/73 (65.75%), Query Frame = 1


Query: 49  CNQKIGECL--TDPEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPG-PA 108
           C+  I EC+   + EM+SEI+RR+L   K YISY +L+R+ VPCSR G SYY C  G  A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILATTK-YISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 109 NSYDRGCEVITRC 119
           N Y RGC  I RC
Sbjct: 107 NPYSRGCSKIARC 118

[Close]

BLAST of CSPI03G10560 vs. NCBI nr
Match: gi|778677782|ref|XP_011650863.1| (PREDICTED: protein RALF-like 24 [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 3.4e-67
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 1


Query: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60
           MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP
Sbjct: 1   MAKSLFLFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTDP 60

Query: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120
           EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR
Sbjct: 61  EMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 120

Query: 121 DVHDINT 128
           DVHDINT
Sbjct: 121 DVHDINT 127

[Close]

BLAST of CSPI03G10560 vs. NCBI nr
Match: gi|659075762|ref|XP_008438316.1| (PREDICTED: protein RALF-like 24 [Cucumis melo])

HSP 1 Score: 256.9 bits (655), Expect = 1.8e-65
Identity = 126/128 (98.44%), Postives = 127/128 (99.22%), Query Frame = 1


Query: 1   MAKSLFLF-FLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMEVMAKGVCNQKIGECLTD 60
           MAKSLFLF FLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEM+VMAKGVCNQKIGECLTD
Sbjct: 1   MAKSLFLFFFLFSIIFPLVFLQTHFSFCDAILDLRSFKGSEMDVMAKGVCNQKIGECLTD 60

Query: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120
           PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA
Sbjct: 61  PEMESEISRRVLMMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCA 120

Query: 121 RDVHDINT 128
           RDVHDINT
Sbjct: 121 RDVHDINT 128

[Close]

BLAST of CSPI03G10560 vs. NCBI nr
Match: gi|596217112|ref|XP_007223934.1| (hypothetical protein PRUPE_ppa013295mg [Prunus persica])

HSP 1 Score: 152.5 bits (384), Expect = 4.9e-34
Identity = 76/115 (66.09%), Postives = 87/115 (75.65%), Query Frame = 1


Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESEISRRVL 
Sbjct: 17  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESEISRRVLA 76

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDINT 128
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR  +DI T
Sbjct: 77  MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCARG-NDIKT 130

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BLAST of CSPI03G10560 vs. NCBI nr
Match: gi|645232307|ref|XP_008222806.1| (PREDICTED: protein RALF-like 24 [Prunus mume])

HSP 1 Score: 149.1 bits (375), Expect = 5.4e-33
Identity = 72/108 (66.67%), Postives = 82/108 (75.93%), Query Frame = 1


Query: 17  LVFLQTHFSFCDAI--LDLRSFKGSEMEVM--AKGVCNQKIGECLTDPEMESEISRRVLM 76
           L+ + THFS C+ +   DL S KGS ++ M  A   CNQKIGECLT+PEMESE SRRVL 
Sbjct: 61  LLLIHTHFSICNGVSAFDLNSLKGSGIDGMVGAGRACNQKIGECLTEPEMESETSRRVLA 120

Query: 77  MQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCAR 121
           MQKKYISY+TLRRD+VPC +PG SYY CH   AN Y RGCEVITRCAR
Sbjct: 121 MQKKYISYETLRRDLVPCGKPGASYYNCHAVAANPYSRGCEVITRCAR 168

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BLAST of CSPI03G10560 vs. NCBI nr
Match: gi|1009177739|ref|XP_015870138.1| (PREDICTED: protein RALF-like 24 [Ziziphus jujuba])

HSP 1 Score: 144.4 bits (363), Expect = 1.3e-31
Identity = 68/115 (59.13%), Postives = 88/115 (76.52%), Query Frame = 1


Query: 17  LVFLQTHFSFCD--AILDLRSFKGSEMEVMAKG-VCNQKIGECLTDPE--MESEISRRVL 76
           L F  THFS C   ++LDL+S K SE+++M  G VCNQK+ +CLT+ E  M+SE +RR+L
Sbjct: 16  LFFSLTHFSICSGVSVLDLKSLKNSEVDMMVGGGVCNQKVEKCLTEVEEMMDSETNRRIL 75

Query: 77  MMQKKYISYDTLRRDMVPCSRPGVSYYECHPGPANSYDRGCEVITRCARDVHDIN 127
            MQK++ISY+TLRRDM+PC+ PG  YY CHPG AN Y+RGCEVITRCAR ++  N
Sbjct: 76  AMQKRFISYETLRRDMIPCATPGAPYYNCHPGAANPYNRGCEVITRCARGINKNN 130

The following BLAST results are available for this feature:

BLAST of CSPI03G10560 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF24_ARATH	1.9e-25	52.76	Protein RALF-like 24 OS=Arabidopsis thaliana GN=RALFL24 PE=3 SV=1	[more]
RLF31_ARATH	9.3e-25	62.79	Protein RALF-like 31 OS=Arabidopsis thaliana GN=RALFL31 PE=3 SV=1	[more]
RALF_TOBAC	1.3e-15	51.22	Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1	[more]
RLF22_ARATH	1.8e-15	55.26	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]
RLF33_ARATH	2.5e-14	46.36	Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1	[more]

BLAST of CSPI03G10560 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7V8_CUCSA	2.3e-67	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129640 PE=4 SV=1	[more]
M5XU33_PRUPE	3.4e-34	66.09	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013295mg PE=4 SV=1	[more]
B9GFJ2_POPTR	2.7e-31	59.82	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s32790g PE=4 SV=2	[more]
A0A067KSY9_JATCU	2.7e-31	60.75	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01083 PE=4 SV=1	[more]
A0A061EJW1_THECC	1.3e-30	60.68	RALFL33, putative OS=Theobroma cacao GN=TCM_020225 PE=4 SV=1	[more]

BLAST of CSPI03G10560 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G23805.1	1.1e-26	52.76	ralf-like 24	[more]
AT4G13950.1	5.2e-26	62.79	ralf-like 31	[more]
AT3G05490.1	9.9e-17	55.26	ralf-like 22	[more]
AT4G15800.1	1.4e-15	46.36	ralf-like 33	[more]
AT1G02900.1	7.1e-15	53.42	rapid alkalinization factor 1	[more]

BLAST of CSPI03G10560 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778677782\|ref\|XP_011650863.1\|	3.4e-67	100.00	PREDICTED: protein RALF-like 24 [Cucumis sativus]	[more]
gi\|659075762\|ref\|XP_008438316.1\|	1.8e-65	98.44	PREDICTED: protein RALF-like 24 [Cucumis melo]	[more]
gi\|596217112\|ref\|XP_007223934.1\|	4.9e-34	66.09	hypothetical protein PRUPE_ppa013295mg [Prunus persica]	[more]
gi\|645232307\|ref\|XP_008222806.1\|	5.4e-33	66.67	PREDICTED: protein RALF-like 24 [Prunus mume]	[more]
gi\|1009177739\|ref\|XP_015870138.1\|	1.3e-31	59.13	PREDICTED: protein RALF-like 24 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G10560.1	CSPI03G10560.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 61..120 score: 2.3
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 1..122 score: 7.9
None	No IPR available	PANTHER	PTHR33136:SF10	PROTEIN RALF-LIKE 24-RELATED	coord: 1..122 score: 7.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G10560	Cla021396	Watermelon (97103) v1	cpiwmB231
CSPI03G10560	Csa3G129640	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G10560	MELO3C006289	Melon (DHL92) v3.5.1	cpimeB230
CSPI03G10560	ClCG05G003080	Watermelon (Charleston Gray)	cpiwcgB226
CSPI03G10560	Lsi05G018650	Bottle gourd (USVL1VR-Ls)	cpilsiB207
CSPI03G10560	CsaV3_3G010700	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G10560	Cla97C05G083710	Watermelon (97103) v2	cpiwmbB209
CSPI03G10560	Bhi01G000140	Wax gourd	cpiwgoB270
CSPI03G10560	Cucsa.256690	Cucumber (Gy14) v1	cgycpiB383
CSPI03G10560	CmaCh06G011970	Cucurbita maxima (Rimu)	cmacpiB826
CSPI03G10560	CmoCh14G019670	Cucurbita moschata (Rifu)	cmocpiB243
CSPI03G10560	CmoCh06G012340	Cucurbita moschata (Rifu)	cmocpiB814
CSPI03G10560	Cp4.1LG03g17800	Cucurbita pepo (Zucchini)	cpecpiB611
CSPI03G10560	Cp4.1LG08g02630	Cucurbita pepo (Zucchini)	cpecpiB861
CSPI03G10560	Carg06309	Silver-seed gourd	carcpiB0998
CSPI03G10560	Carg14912	Silver-seed gourd	carcpiB0651

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G10560	Watermelon (97103) v2	cpiwmbB239
CSPI03G10560	Wild cucumber (PI 183967)	cpicpiB063
CSPI03G10560	Cucurbita maxima (Rimu)	cmacpiB255
CSPI03G10560	Cucurbita maxima (Rimu)	cmacpiB361
CSPI03G10560	Cucurbita moschata (Rifu)	cmocpiB353
CSPI03G10560	Cucurbita moschata (Rifu)	cmocpiB883
CSPI03G10560	Watermelon (Charleston Gray)	cpiwcgB255
CSPI03G10560	Cucurbita pepo (Zucchini)	cpecpiB136
CSPI03G10560	Bottle gourd (USVL1VR-Ls)	cpilsiB149
CSPI03G10560	Melon (DHL92) v3.6.1	cpimedB224
CSPI03G10560	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G10560	Silver-seed gourd	carcpiB0261
CSPI03G10560	Silver-seed gourd	carcpiB1115