Cucsat.G20249 (gene) Cucumber (B10) v3

Overview
NameCucsat.G20249
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptiontRNA (Adenine(37)-N6)-methyltransferase
Locationctg47: 286202 .. 289931 (-)
RNA-Seq ExpressionCucsat.G20249
SyntenyCucsat.G20249
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGGTATTTGGGTAATTGTCCAAAATAAAACCCCAAAACCGGAAATTGGTTAAACCTCAAGCAGCCGGATCGAGTTCGTCTGAACGCTGCAGACTAATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCGTAAAACTTCGAAACTTTTTTTTAGATCATATATGTCCTCTTTCTTAGTTTCTCCGATTTCCGATTTGTTTCTTACAATTAAAGTTTTGTTGTTTTCCCCAGTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGTACTTCATATCATTTGCTCTTCTGCATCGCTTCATGTTCCAGTGTTGTTATTTGATATCTTGGTTTCAATTTTCTCCCTACTTTAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGTTAGTTTTTTTATTGCATTATCTGGTAGTTGAAACATGAATTTTTGGGTCGTTGTTTTCTCATTTTTCTGTTCATAAAGCTAATGTCAAAAGTGGATATGTGTGAGCCATATTCTTCAGTTAGCTGTAAAAATCGAACAATCAATAACCTTTTTTGGGGGTTTTCTTTTTAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGTACGTTTTCTTCATTAGTTGATGCTCCTTCAATGTGGAACTCATATTTTTATTATTCTTATAATTTCCTAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACGAGATCTCCACATAGACCATGCCCAATCGGACTTACTGTTGCAAAGGTAAGATAATTTTTTTTAGTGCTGTCGATGTGTCTTGCAAGTTTATGGAGAAGCCCATGGTAGATCACAGACGTGGATGAAATGTGTCTTCCCCTAACGAGTTCTAGATGGGTTTTGCTGTCACATGAACATGGTCCAACATGTTTTGTATGGTTAGGCTGAAATGTCATTTAATTTAGTATTAGAAATTCATGGTTACAAGCTTGCAGTTGTTGTAACTTGCAAGTGTTAGACAGCATTCAGATGCAACAAATAAATGGGGGAATATTAAATCTAAGATTAATAGATCTGCTATGTTACCAATTCAGGTGGAGGCTGTCCAGGGACATGAAATTCTACTCTCTGGTGCCGACCTTGTTGATGGAACTGTAAGAGTTCGTTTTCAATTTTTCTTACCACGAAAAAGGTCTAGTTGAATTTATACGTTACTTCTTGGGGTAATGATTGTTATGATTTCTTGCTTTTTTTGAATTTTGTTACAGCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAAAATGCAACAGTCCCAAAGTGGTTACTGGTATGTCTTCAAGAATTAATTTCTATGACTAAACGTTATTTTAGAATTTCCTCGATGCTTATGTTATTGCTATTCATCGTTTAAATTGTTTTTTATGTTTTAGTTTTTGGCAACTATTCTTTCAGTCTGTCCCAACGAAGATTTATTCTGAATGTTGTGCTTTTCAAACTTCTCTTATGCATGGCATATGTGATATGACTAGTTGTGTGCAAATAACTTAAGAAATTCAGCTTTCCATATACTATTAGGTTTTTGTCCTACTCTCATATTTGGAGTTATACGCTGTGACCATGTAATATGTTTATGTACATTCCTCTTGGCATTCTTACATGTTATTTACGCCAACACAGGGGGACAATGTATTGACTGTTGCGTCCATTTGCTTCTCTGAAGAATTTACGTCCACTCTATCCAAGTGTTGGCCACTAACGGTGAGTTCCTTTGTCTTTTTCTTTATAATTAATTGATCATTGTTGTTTCATAATCATCATTATTATTGTTACTACTACATAGATATTCATTAGTGTTCGGGCCAACTTGGAATATTCGACAATATGCCAGAAATATTCTCTGATTGTTTTGCACAGGACATGGTAAAATCAATACACAACAATATTTTACTTAGCGTGCATAAGTTTTCCTTAAAATTTTGGGTATTGGAAAGGTGTAATGTAAAATCAAGAATCTAGTTTCTTTGGTTTCAAAAGGCACTTTTCAAATTGCTATATTTTATACCTGAAGGGGAGGGGAGGGGAGGGACGGGACGTCCTTGCTGGAGTTTTGGGTCATAATCAGCCTTAGGTTGGTCCTCTGATAGAAAACTTAATAAAAGTGAAAGCCTTTGTTGTCTTCTGGTGATATTGTAGCACAAGGAGTCTAAATGCCTAAAGATAATTACGCCTCTGGAATTTTACATTATGAACGTTGGGAGAGCGGCTGCTTTGTAGTCTGATTACCATTTTTGTGAAACTCCACGTTACAAAGAGCTAATGCTCTCAACTTTCATATGGTACAAAGTAGGATATTCTGGATGGGGTATGCCATTTAGGGGTGTTTAGTGGTTCGTAATGGGATGTGTTTGTAATGTAATGTAATCCAAAATCCATATTTGGATAGAGTGTTTTGGACTCAACTTATAATACAAACTTCATTCTGCTTAATAGTTTTCAATCCAACGTTCTCTCTCCCTGGTCTCATTTCTAACACTCATTTTTTATTTTTTATTTTCAGTAATGTAATCAGTCCAACTGTCTAAATAACTCTTTAATTTTTTCTTTGGTTTCCATCATTTTGCCACATAAATTTCTCACGCATCATATTTTGTTGGTTTCAGGAAAAGAAATCACTTTATGCGTCGGAAGATGAATTGAAAAACTTTGTGAAACAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGAAGTCGACCTCATTGCTCTCACATTAAGACGGATTCAGATGATAGTTCAGATTCAGATGGTCACCTAAATGACAAAGTTTCGAGTAGTTCACAAAGTTCTTCAAAGAGCACAATCTATCACCTACTTGTAGATGGCTTAGATGTTTCATACACAATTGGTTGCCATGGGGACGTGGTTGTGGACAAAGTGGAACTTGCATCATCTAGCTCGAGAACAAGAAGCAATTAACACAAAGCAAATTCTTCCGAATAGGCAAATCAGGGTTGTCTTATTACTAACCTTGCATTGCTACAATTAAAAGGATCCCCTATCTTGGAAATTTTCCCTTGAAATTATACTGCCTTGAATGGAGCTTGATCTTGCTCATCTTTCTGTCAGAAACGAACTGTAGGTTGAAACTGAGACTAGAATGCTGGATAAATTATGAGTTAAATGGTTTTGGTTGAAAAGCAGCTGTTTGCTTACGAGTGATTCTTTGACTTTTATACCACAATCTTAAAACAAGAAAAGGATATGTTTGTGACCTCTGTACTCCAGGAGTAGCACAAGTGACTAGGTCACTATAGGCTGAACTGATTCACGTTTGGTTTGGATTTTGCTGTTTTTCATTTTTAGTTTAGAAAATTCTTTGCTTAGGTTTCTTTCTCTCTCTCAATTGAAAAAACAACTTTTCAATCATTTGCCCGAA

Coding sequence (CDS)

ATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACGAGATCTCCACATAGACCATGCCCAATCGGACTTACTGTTGCAAAGGTGGAGGCTGTCCAGGGACATGAAATTCTACTCTCTGGTGCCGACCTTGTTGATGGAACTCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAAAATGCAACAGTCCCAAAGTGGTTACTGGGGGACAATGTATTGACTGTTGCGTCCATTTGCTTCTCTGAAGAATTTACGTCCACTCTATCCAAGTGTTGGCCACTAACGGAAAAGAAATCACTTTATGCGTCGGAAGATGAATTGAAAAACTTTGTGAAACAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGAAGTCGACCTCATTGCTCTCACATTAAGACGGATTCAGATGATAGTTCAGATTCAGATGGTCACCTAAATGACAAAGTTTCGAGTAGTTCACAAAGTTCTTCAAAGAGCACAATCTATCACCTACTTGTAGATGGCTTAGATGTTTCATACACAATTGGTTGCCATGGGGACGTGGTTGTGGACAAAGTGGAACTTGCATCATCTAGCTCGAGAACAAGAAGCAATTAA

Protein sequence

MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAERQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTPVLDVKPYLPYCDSVQNATVPKWLLGDNVLTVASICFSEEFTSTLSKCWPLTEKKSLYASEDELKNFVKQVLSWDIRSVSQRSRPHCSHIKTDSDDSSDSDGHLNDKVSSSSQSSSKSTIYHLLVDGLDVSYTIGCHGDVVVDKVELASSSSRTRSN
Homology
[Close]
BLAST of Cucsat.G20249 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 2.0e-07
Identity = 27/64 (42.19%), Postives = 40/64 (62.50%), Query Frame = 0 

Query: 98  PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 157
           P+G ++S F+ +NGTPRQ  +   +RA L    RI   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 158 NTDL 159
           N  L
Sbjct: 92  NGHL 95

[Close]
BLAST of Cucsat.G20249 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 4.9e-06
Identity = 27/65 (41.54%), Postives = 38/65 (58.46%), Query Frame = 0 

Query: 98  PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 157
           PIG ++S F  + GTPRQ  +   +RA L     I   P  S+ GL E+SH W+++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 158 NTDLD 160
           N  L+
Sbjct: 92  NGHLN 96

[Close]
BLAST of Cucsat.G20249 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.4e-06
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 0 

Query: 98  PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 157
           PIG ++S F+ + GTPRQ  +   +RA L     I   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 158 NTDLD 160
           N  L+
Sbjct: 92  NGHLN 96

[Close]
BLAST of Cucsat.G20249 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 316 bits (810), Expect = 3.84e-105
Identity = 167/168 (99.40%), Postives = 168/168 (100.00%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. NCBI nr
Match: XP_038879627.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 301 bits (770), Expect = 1.65e-100
Identity = 156/168 (92.86%), Postives = 166/168 (98.81%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 301 bits (770), Expect = 5.22e-99
Identity = 156/168 (92.86%), Postives = 166/168 (98.81%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. NCBI nr
Match: XP_008453658.1 (PREDICTED: uncharacterized protein LOC103494312 isoform X2 [Cucumis melo])

HSP 1 Score: 300 bits (767), Expect = 5.56e-99
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 300 bits (767), Expect = 1.49e-98
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 316 bits (810), Expect = 1.86e-105
Identity = 167/168 (99.40%), Postives = 168/168 (100.00%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match: A0A1S3BWW3 (uncharacterized protein LOC103494312 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 2.69e-99
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 7.20e-99
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 7.20e-99
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK+
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKV 168

[Close]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 288 bits (738), Expect = 1.78e-94
Identity = 146/168 (86.90%), Postives = 163/168 (97.02%), Query Frame = 0 

Query: 7   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 66
           M Y A+SRWSTAAMS+ LAFISASAI+SLYLVT+KSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 67  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 126
           RQGRIRAQQALRKA+A+PKSD+V+CTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 127 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKL 174
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAK+
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKV 168

The following BLAST results are available for this feature:
BLAST of Cucsat.G20249 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9BU702.0e-0742.19tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=... [more]
Q562D64.9e-0641.54tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV... [more]
Q4V7E06.4e-0640.00tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE... [more]
BLAST of Cucsat.G20249 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004149885.13.84e-10599.40uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ... [more]
XP_038879627.11.65e-10092.86tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida][more]
XP_038879626.15.22e-9992.86tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida][more]
XP_008453658.15.56e-9994.64PREDICTED: uncharacterized protein LOC103494312 isoform X2 [Cucumis melo][more]
XP_008453657.11.49e-9894.64PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo][more]
BLAST of Cucsat.G20249 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTM61.86e-10599.40TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P... [more]
A0A1S3BWW32.69e-9994.64uncharacterized protein LOC103494312 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3CC847.20e-9994.64tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A1S3BW927.20e-9994.64tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1BYL51.78e-9486.90uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036414YaeB, N-terminal domain superfamilyGENE3D2.40.30.70coord: 2..49
e-value: 1.4E-13
score: 52.7
IPR023370TrmO-like, N-terminal domainPFAMPF01980TrmOcoord: 4..38
e-value: 1.2E-10
score: 41.6
IPR023370TrmO-like, N-terminal domainPROSITEPS51668TSAA_2coord: 1..42
score: 22.631029
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 111..128
NoneNo IPR availablePANTHERPTHR12818:SF0TRNA (ADENINE(37)-N6)-METHYLTRANSFERASEcoord: 5..172
IPR040372YaeB-likePANTHERPTHR12818UNCHARACTERIZEDcoord: 5..172
IPR036413YaeB-like superfamilySUPERFAMILY118196YaeB-likecoord: 4..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G20249.T1Cucsat.G20249.T1mRNA
Cucsat.G20249.T3Cucsat.G20249.T3mRNA
Cucsat.G20249.T2Cucsat.G20249.T2mRNA
Cucsat.G20249.T4Cucsat.G20249.T4mRNA
Cucsat.G20249.T7Cucsat.G20249.T7mRNA
Cucsat.G20249.T11Cucsat.G20249.T11mRNA
Cucsat.G20249.T10Cucsat.G20249.T10mRNA
Cucsat.G20249.T12Cucsat.G20249.T12mRNA
Cucsat.G20249.T14Cucsat.G20249.T14mRNA
Cucsat.G20249.T9Cucsat.G20249.T9mRNA
Cucsat.G20249.T8Cucsat.G20249.T8mRNA
Cucsat.G20249.T6Cucsat.G20249.T6mRNA
Cucsat.G20249.T13Cucsat.G20249.T13mRNA
Cucsat.G20249.T17Cucsat.G20249.T17mRNA
Cucsat.G20249.T18Cucsat.G20249.T18mRNA
Cucsat.G20249.T20Cucsat.G20249.T20mRNA
Cucsat.G20249.T5Cucsat.G20249.T5mRNA
Cucsat.G20249.T16Cucsat.G20249.T16mRNA
Cucsat.G20249.T22Cucsat.G20249.T22mRNA
Cucsat.G20249.T21Cucsat.G20249.T21mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016430 tRNA (adenine-N6-)-methyltransferase activity