Cucsat.G20249.T14 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G20249.T14
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptiontRNA (Adenine(37)-N6)-methyltransferase
Locationctg47: 286202 .. 289967 (-)
RNA-Seq ExpressionCucsat.G20249.T14
SyntenyCucsat.G20249.T14
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGGTTAAACCTCAAGCAGCCGGATCGAGTTCGTCTGAACGCTGCAGACTAATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCGTAAAACTTCGAAACTTTTTTTTAGATCATATATGTCCTCTTTCTTAGTTTCTCCGATTTCCGATTTGTTTCTTACAATTAAAGTTTTGTTGTTTTCCCCAGTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGTACTTCATATCATTTGCTCTTCTGCATCGCTTCATGTTCCAGTGTTGTTATTTGATATCTTGGTTTCAATTTTCTCCCTACTTTAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGTTAGTTTTTTTATTGCATTATCTGGTAGTTGAAACATGAATTTTTGGGTCGTTGTTTTCTCATTTTTCTGTTCATAAAGCTAATGTCAAAAGTGGATATGTGTGAGCCATATTCTTCAGTTAGCTGTAAAAATCGAACAATCAATAACCTTTTTTGGGGGTTTTCTTTTTAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGTACGTTTTCTTCATTAGTTGATGCTCCTTCAATGTGGAACTCATATTTTTATTATTCTTATAATTTCCTAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACGAGATCTCCACATAGACCATGCCCAATCGGACTTACTGTTGCAAAGGTAAGATAATTTTTTTTAGTGCTGTCGATGTGTCTTGCAAGTTTATGGAGAAGCCCATGGTAGATCACAGACGTGGATGAAATGTGTCTTCCCCTAACGAGTTCTAGATGGGTTTTGCTGTCACATGAACATGGTCCAACATGTTTTGTATGGTTAGGCTGAAATGTCATTTAATTTAGTATTAGAAATTCATGGTTACAAGCTTGCAGTTGTTGTAACTTGCAAGTGTTAGACAGCATTCAGATGCAACAAATAAATGGGGGAATATTAAATCTAAGATTAATAGATCTGCTATGTTACCAATTCAGGTGGAGGCTGTCCAGGGACATGAAATTCTACTCTCTGGTGCCGACCTTGTTGATGGAACTGTAAGAGTTCGTTTTCAATTTTTCTTACCACGAAAAAGGTCTAGTTGAATTTATACGTTACTTCTTGGGGTAATGATTGTTATGATTTCTTGCTTTTTTTGAATTTTGTTACAGCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAAAATGCAACAGTCCCAAAGTGGTTACTGGTATGTCTTCAAGAATTAATTTCTATGACTAAACGTTATTTTAGAATTTCCTCGATGCTTATGTTATTGCTATTCATCGTTTAAATTGTTTTTTATGTTTTAGTTTTTGGCAACTATTCTTTCAGTCTGTCCCAACGAAGATTTATTCTGAATGTTGTGCTTTTCAAACTTCTCTTATGCATGGCATATGTGATATGACTAGTTGTGTGCAAATAACTTAAGAAATTCAGCTTTCCATATACTATTAGGTTTTTGTCCTACTCTCATATTTGGAGTTATACGCTGTGACCATGTAATATGTTTATGTACATTCCTCTTGGCATTCTTACATGTTATTTACGCCAACACAGGGGGACAATGTATTGACTGTTGCGTCCATTTGCTTCTCTGAAGAATTTACGTCCACTCTATCCAAGTGTTGGCCACTAACGGTGAGTTCCTTTGTCTTTTTCTTTATAATTAATTGATCATTGTTGTTTCATAATCATCATTATTATTGTTACTACTACATAGATATTCATTAGTGTTCGGGCCAACTTGGAATATTCGACAATATGCCAGAAATATTCTCTGATTGTTTTGCACAGGACATGGTAAAATCAATACACAACAATATTTTACTTAGCGTGCATAAGTTTTCCTTAAAATTTTGGGTATTGGAAAGGTGTAATGTAAAATCAAGAATCTAGTTTCTTTGGTTTCAAAAGGCACTTTTCAAATTGCTATATTTTATACCTGAAGGGGAGGGGAGGGGAGGGACGGGACGTCCTTGCTGGAGTTTTGGGTCATAATCAGCCTTAGGTTGGTCCTCTGATAGAAAACTTAATAAAAGTGAAAGCCTTTGTTGTCTTCTGGTGATATTGTAGCACAAGGAGTCTAAATGCCTAAAGATAATTACGCCTCTGGAATTTTACATTATGAACGTTGGGAGAGCGGCTGCTTTGTAGTCTGATTACCATTTTTGTGAAACTCCACGTTACAAAGAGCTAATGCTCTCAACTTTCATATGGTACAAAGTAGGATATTCTGGATGGGGTATGCCATTTAGGGGTGTTTAGTGGTTCGTAATGGGATGTGTTTGTAATGTAATGTAATCCAAAATCCATATTTGGATAGAGTGTTTTGGACTCAACTTATAATACAAACTTCATTCTGCTTAATAGTTTTCAATCCAACGTTCTCTCTCCCTGGTCTCATTTCTAACACTCATTTTTTATTTTTTATTTTCAGTAATGTAATCAGTCCAACTGTCTAAATAACTCTTTAATTTTTTCTTTGGTTTCCATCATTTTGCCACATAAATTTCTCACGCATCATATTTTGTTGGTTTCAGGAAAAGAAATCACTTTATGCGTCGGAAGATGAATTGAAAAACTTTGTGAAACAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGAAGTCGACCTCATTGCTCTCACATTAAGACGGATTCAGATGATAGTTCAGATTCAGATGGTCACCTAAATGACAAAGTTTCGAGTAGTTCACAAAGTTCTTCAAAGAGCACAATCTATCACCTACTTGTAGATGGCTTAGATGTTTCATACACAATTGGTTGCCATGGGGACGTGGTTGTGGACAAAGTGGAACTTGCATCATCTAGCTCGAGAACAAGAAGCAATTAACACAAAGCAAATTCTTCCGAATAGGCAAATCAGGGTTGTCTTATTACTAACCTTGCATTGCTACAATTAAAAGGATCCCCTATCTTGGAAATTTTCCCTTGAAATTATACTGCCTTGAATGGAGCTTGATCTTGCTCATCTTTCTGTCAGAAACGAACTGTAGGTTGAAACTGAGACTAGAATGCTGGATAAATTATGAGTTAAATGGTTTTGGTTGAAAAGCAGCTGTTTGCTTACGAGTGATTCTTTGACTTTTATACCACAATCTTAAAACAAGAAAAGGATATGTTTGTGACCTCTGTACTCCAGGAGTAGCACAAGTGACTAGGTCACTATAGGCTGAACTGATTCACGTTTGGTTTGGATTTTGCTGTTTTTCATTTTTAGTTTAGAAAATTCTTTGCTTAGGTTTCTTTCTCTCTCTCAATTGAAAAAACAACTTTTCAA

Coding sequence (CDS)

AAATTGGTTAAACCTCAAGCAGCCGGATCGAGTTCGTCTGAACGCTGCAGACTAATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACGAGATCTCCACATAGACCATGCCCAATCGGACTTACTGTTGCAAAGGTGGAGGCTGTCCAGGGACATGAAATTCTACTCTCTGGTGCCGACCTTGTTGATGGAACTTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAAAATGCAACAGTCCCAAAGTGGTTACTGGGGGACAATGTATTGA

Protein sequence

KLVKPQAAGSSSSERCRLMTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAERQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGTYLMLNLIYHTVTVSKMQQSQSGYWGTMY
Homology
[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 116.3 bits (290), Expect = 5.2e-25
Identity = 61/130 (46.92%), Postives = 82/130 (63.08%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           P+G ++S F+ +NGTPRQ  +   +RA L    RI   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 170 NTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTVAKVEAVQGHEIL 229
           N  L            KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  I 
Sbjct: 92  NGHL----------SCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIY 151

Query: 230 LSGADLVDGT 237
           LSG D++ GT
Sbjct: 152 LSGIDMIHGT 151

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 116.3 bits (290), Expect = 5.2e-25
Identity = 61/130 (46.92%), Postives = 82/130 (63.08%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           PIG ++S F  + GTPRQ  +   +RA L     I   P  S+ GL E+SH W+++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 170 NTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTVAKVEAVQGHEIL 229
           N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 230 LSGADLVDGT 237
           LSG D++DGT
Sbjct: 152 LSGVDMIDGT 151

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.8e-24
Identity = 59/130 (45.38%), Postives = 82/130 (63.08%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           PIG ++S F+ + GTPRQ  +   +RA L     I   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 170 NTDLDKLWKDPSKSKFKAKVRVPRLKGERVGVFATRSPHRPCPIGLTVAKVEAVQGHEIL 229
           N  L+          +KAKV+ PRL G + GVF+TRSPHRP  IGLT+AK+E V+G  + 
Sbjct: 92  NGHLN----------YKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAVY 151

Query: 230 LSGADLVDGT 237
           LSG D++ GT
Sbjct: 152 LSGIDMIHGT 151

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match: Q9RPT0 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=rcsF PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.0e-18
Identity = 55/137 (40.15%), Postives = 79/137 (57.66%), Query Frame = 0 

Query: 105 SYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARIPP----ASIEGLGEYSHCWV 164
           ++ ++PIG I+S F  +   PRQ LL P AR  L     +PP     ++EGL + SH W+
Sbjct: 2   THSVSPIGYIRSCFMEKFAIPRQPLLAPAARGTLEL---LPPFDQVEALEGLEQVSHVWL 61

Query: 165 IYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGER-VGVFATRSPHRPCPIGLTVAKVEA 224
           +++FH             + K + KVR PRL G R +GVFATR+ HRP  IG +V ++E 
Sbjct: 62  LFLFH----------QALEDKPRLKVRPPRLGGNRSLGVFATRATHRPNGIGQSVVRLEG 121

Query: 225 VQGHEILLSGADLVDGT 237
            +   + LSG DL+DGT
Sbjct: 122 FEAGRLWLSGIDLLDGT 125

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match: O68638 (Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas aeruginosa OX=287 GN=rcsF PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 54/137 (39.42%), Postives = 78/137 (56.93%), Query Frame = 0 

Query: 105 SYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARIPP----ASIEGLGEYSHCWV 164
           S+ ++PIG ++S F  +   PRQ  L P A   L     +PP     ++EGL + SH W+
Sbjct: 2   SHSISPIGHVRSCFKEKFAIPRQPQLAPAATGVLEL---LPPFDTGDAVEGLEQVSHVWL 61

Query: 165 IYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKG-ERVGVFATRSPHRPCPIGLTVAKVEA 224
           I++FH             + K + KVR PRL G + +GVFATR+ HRP  +G +V K+E 
Sbjct: 62  IFLFH----------QALEDKPRLKVRPPRLGGNQSMGVFATRATHRPNGLGQSVVKLEK 121

Query: 225 VQGHEILLSGADLVDGT 237
           V+   + LSG DL+DGT
Sbjct: 122 VEPGRLWLSGIDLLDGT 125

[Close]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 415 bits (1066), Expect = 1.34e-142
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match: XP_038879627.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 397 bits (1021), Expect = 2.75e-137
Identity = 204/218 (93.58%), Postives = 216/218 (99.08%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 398 bits (1023), Expect = 5.45e-136
Identity = 210/218 (96.33%), Postives = 213/218 (97.71%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match: TYK09463.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 398 bits (1023), Expect = 5.45e-136
Identity = 210/218 (96.33%), Postives = 213/218 (97.71%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 397 bits (1021), Expect = 1.10e-135
Identity = 204/218 (93.58%), Postives = 216/218 (99.08%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 415 bits (1066), Expect = 6.50e-143
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 398 bits (1023), Expect = 2.64e-136
Identity = 210/218 (96.33%), Postives = 213/218 (97.71%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 398 bits (1023), Expect = 2.64e-136
Identity = 210/218 (96.33%), Postives = 213/218 (97.71%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 384 bits (985), Expect = 1.56e-130
Identity = 193/218 (88.53%), Postives = 213/218 (97.71%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M Y A+SRWSTAAMS+ LAFISASAI+SLYLVT+KSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKA+A+PKSD+V+CTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAKVRVPRLKG+RVGV
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAKVRVPRLKGKRVGV 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIGLT+AKVEA+QGH+ILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGLTIAKVEAIQGHQILLSGADLVDGT 218

[Close]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match: A0A6J1HPV8 (uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC111464972 PE=4 SV=1)

HSP 1 Score: 379 bits (972), Expect = 1.24e-128
Identity = 194/218 (88.99%), Postives = 211/218 (96.79%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA SR STAAMSVTLAF+SASAI+SLY VTKKSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MAYAAVSRSSTAAMSVTLAFVSASAILSLYFVTKKSRELGSKVRELEASLAASLKKTIAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAV+EPKSDSV+CT YPM PIGTIQSSF+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKAVSEPKSDSVECTLYPMAPIGTIQSSFSTRNGTPRQSLVVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVRVPRLKGERVGV 198
           VFS+RIPPASIEGLGEYSHCWVIYVFHLNT+LDKLWKDPSKSKFKAKV+VPRLKGE+VG+
Sbjct: 121 VFSSRIPPASIEGLGEYSHCWVIYVFHLNTNLDKLWKDPSKSKFKAKVKVPRLKGEKVGI 180

Query: 199 FATRSPHRPCPIGLTVAKVEAVQGHEILLSGADLVDGT 236
           FATRSPHRPCPIG+T+AKVEA+QGH+ILLSGADLVDGT
Sbjct: 181 FATRSPHRPCPIGITIAKVEAIQGHQILLSGADLVDGT 218

The following BLAST results are available for this feature:
BLAST of Cucsat.G20249.T14 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9BU705.2e-2546.92tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=... [more]
Q562D65.2e-2546.92tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV... [more]
Q4V7E05.8e-2445.38tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE... [more]
Q9RPT08.0e-1840.15Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae... [more]
O686381.5e-1639.42Putative S-adenosylmethionine-dependent methyltransferase RcsF OS=Pseudomonas ae... [more]
BLAST of Cucsat.G20249.T14 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004149885.11.34e-142100.00uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ... [more]
XP_038879627.12.75e-13793.58tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida][more]
XP_008453657.15.45e-13696.33PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo][more]
TYK09463.15.45e-13696.33tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo var. makuwa][more]
XP_038879626.11.10e-13593.58tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida][more]
BLAST of Cucsat.G20249.T14 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTM66.50e-143100.00TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P... [more]
A0A5D3CC842.64e-13696.33tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A1S3BW922.64e-13696.33tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1BYL51.56e-13088.53uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A6J1HPV81.24e-12888.99uncharacterized protein LOC111464972 OS=Cucurbita moschata OX=3662 GN=LOC1114649... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036414YaeB, N-terminal domain superfamilyGENE3D2.40.30.70coord: 107..240
e-value: 2.0E-41
score: 143.1
IPR023370TrmO-like, N-terminal domainPFAMPF01980TrmOcoord: 123..238
e-value: 1.1E-33
score: 115.9
IPR023370TrmO-like, N-terminal domainPROSITEPS51668TSAA_2coord: 108..253
score: 52.495564
IPR023370TrmO-like, N-terminal domainCDDcd09281UPF0066coord: 110..236
e-value: 6.28045E-44
score: 143.016
IPR040372YaeB-likePANTHERPTHR12818UNCHARACTERIZEDcoord: 26..236
IPR036413YaeB-like superfamilySUPERFAMILY118196YaeB-likecoord: 104..236

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G20249Cucsat.G20249gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T14.E1Cucsat.G20249.T14.E1exon
Cucsat.G20249.T14.E2Cucsat.G20249.T14.E2exon
Cucsat.G20249.T14.E3Cucsat.G20249.T14.E3exon
Cucsat.G20249.T14.E4Cucsat.G20249.T14.E4exon
Cucsat.G20249.T14.E5Cucsat.G20249.T14.E5exon
Cucsat.G20249.T14.E6Cucsat.G20249.T14.E6exon
Cucsat.G20249.T14.E7Cucsat.G20249.T14.E7exon
Cucsat.G20249.T14.E8Cucsat.G20249.T14.E8exon
Cucsat.G20249.T14.E9Cucsat.G20249.T14.E9exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T14.C8Cucsat.G20249.T14.C8CDS
Cucsat.G20249.T14.C7Cucsat.G20249.T14.C7CDS
Cucsat.G20249.T14.C6Cucsat.G20249.T14.C6CDS
Cucsat.G20249.T14.C5Cucsat.G20249.T14.C5CDS
Cucsat.G20249.T14.C4Cucsat.G20249.T14.C4CDS
Cucsat.G20249.T14.C3Cucsat.G20249.T14.C3CDS
Cucsat.G20249.T14.C2Cucsat.G20249.T14.C2CDS
Cucsat.G20249.T14.C1Cucsat.G20249.T14.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T14Cucsat.G20249.T14-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016430 tRNA (adenine-N6-)-methyltransferase activity