Cucsat.G20249.T10 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G20249.T10
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptiontRNA (Adenine(37)-N6)-methyltransferase
Locationctg47: 286202 .. 289967 (-)
RNA-Seq ExpressionCucsat.G20249.T10
SyntenyCucsat.G20249.T10
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGGTTAAACCTCAAGCAGCCGGATCGAGTTCGTCTGAACGCTGCAGACTAATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCGTAAAACTTCGAAACTTTTTTTTAGATCATATATGTCCTCTTTCTTAGTTTCTCCGATTTCCGATTTGTTTCTTACAATTAAAGTTTTGTTGTTTTCCCCAGTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGTACTTCATATCATTTGCTCTTCTGCATCGCTTCATGTTCCAGTGTTGTTATTTGATATCTTGGTTTCAATTTTCTCCCTACTTTAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGTTAGTTTTTTTATTGCATTATCTGGTAGTTGAAACATGAATTTTTGGGTCGTTGTTTTCTCATTTTTCTGTTCATAAAGCTAATGTCAAAAGTGGATATGTGTGAGCCATATTCTTCAGTTAGCTGTAAAAATCGAACAATCAATAACCTTTTTTGGGGGTTTTCTTTTTAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGTACGTTTTCTTCATTAGTTGATGCTCCTTCAATGTGGAACTCATATTTTTATTATTCTTATAATTTCCTAAAATCCCATAAAAGGACAGCTCAATGGTAGGTTAGAGTACCGAGGTTGAAAGGCGAAAGAGTAGGAGTCTTTGCTACGAGATCTCCACATAGACCATGCCCAATCGGACTTACTGTTGCAAAGGTAAGATAATTTTTTTTAGTGCTGTCGATGTGTCTTGCAAGTTTATGGAGAAGCCCATGGTAGATCACAGACGTGGATGAAATGTGTCTTCCCCTAACGAGTTCTAGATGGGTTTTGCTGTCACATGAACATGGTCCAACATGTTTTGTATGGTTAGGCTGAAATGTCATTTAATTTAGTATTAGAAATTCATGGTTACAAGCTTGCAGTTGTTGTAACTTGCAAGTGTTAGACAGCATTCAGATGCAACAAATAAATGGGGGAATATTAAATCTAAGATTAATAGATCTGCTATGTTACCAATTCAGGTGGAGGCTGTCCAGGGACATGAAATTCTACTCTCTGGTGCCGACCTTGTTGATGGAACTGTAAGAGTTCGTTTTCAATTTTTCTTACCACGAAAAAGGTCTAGTTGAATTTATACGTTACTTCTTGGGGTAATGATTGTTATGATTTCTTGCTTTTTTTGAATTTTGTTACAGCCAGTACTTGATGTTAAACCTTATCTACCATACTGTGACAGTGTCCAAAATGCAACAGTCCCAAAGTGGTTACTGGTATGTCTTCAAGAATTAATTTCTATGACTAAACGTTATTTTAGAATTTCCTCGATGCTTATGTTATTGCTATTCATCGTTTAAATTGTTTTTTATGTTTTAGTTTTTGGCAACTATTCTTTCAGTCTGTCCCAACGAAGATTTATTCTGAATGTTGTGCTTTTCAAACTTCTCTTATGCATGGCATATGTGATATGACTAGTTGTGTGCAAATAACTTAAGAAATTCAGCTTTCCATATACTATTAGGTTTTTGTCCTACTCTCATATTTGGAGTTATACGCTGTGACCATGTAATATGTTTATGTACATTCCTCTTGGCATTCTTACATGTTATTTACGCCAACACAGGGGGACAATGTATTGACTGTTGCGTCCATTTGCTTCTCTGAAGAATTTACGTCCACTCTATCCAAGTGTTGGCCACTAACGGTGAGTTCCTTTGTCTTTTTCTTTATAATTAATTGATCATTGTTGTTTCATAATCATCATTATTATTGTTACTACTACATAGATATTCATTAGTGTTCGGGCCAACTTGGAATATTCGACAATATGCCAGAAATATTCTCTGATTGTTTTGCACAGGACATGGTAAAATCAATACACAACAATATTTTACTTAGCGTGCATAAGTTTTCCTTAAAATTTTGGGTATTGGAAAGGTGTAATGTAAAATCAAGAATCTAGTTTCTTTGGTTTCAAAAGGCACTTTTCAAATTGCTATATTTTATACCTGAAGGGGAGGGGAGGGGAGGGACGGGACGTCCTTGCTGGAGTTTTGGGTCATAATCAGCCTTAGGTTGGTCCTCTGATAGAAAACTTAATAAAAGTGAAAGCCTTTGTTGTCTTCTGGTGATATTGTAGCACAAGGAGTCTAAATGCCTAAAGATAATTACGCCTCTGGAATTTTACATTATGAACGTTGGGAGAGCGGCTGCTTTGTAGTCTGATTACCATTTTTGTGAAACTCCACGTTACAAAGAGCTAATGCTCTCAACTTTCATATGGTACAAAGTAGGATATTCTGGATGGGGTATGCCATTTAGGGGTGTTTAGTGGTTCGTAATGGGATGTGTTTGTAATGTAATGTAATCCAAAATCCATATTTGGATAGAGTGTTTTGGACTCAACTTATAATACAAACTTCATTCTGCTTAATAGTTTTCAATCCAACGTTCTCTCTCCCTGGTCTCATTTCTAACACTCATTTTTTATTTTTTATTTTCAGTAATGTAATCAGTCCAACTGTCTAAATAACTCTTTAATTTTTTCTTTGGTTTCCATCATTTTGCCACATAAATTTCTCACGCATCATATTTTGTTGGTTTCAGGAAAAGAAATCACTTTATGCGTCGGAAGATGAATTGAAAAACTTTGTGAAACAGGTGCTTTCTTGGGATATTCGATCAGTGTCTCAACGAAGTCGACCTCATTGCTCTCACATTAAGACGGATTCAGATGATAGTTCAGATTCAGATGGTCACCTAAATGACAAAGTTTCGAGTAGTTCACAAAGTTCTTCAAAGAGCACAATCTATCACCTACTTGTAGATGGCTTAGATGTTTCATACACAATTGGTTGCCATGGGGACGTGGTTGTGGACAAAGTGGAACTTGCATCATCTAGCTCGAGAACAAGAAGCAATTAACACAAAGCAAATTCTTCCGAATAGGCAAATCAGGGTTGTCTTATTACTAACCTTGCATTGCTACAATTAAAAGGATCCCCTATCTTGGAAATTTTCCCTTGAAATTATACTGCCTTGAATGGAGCTTGATCTTGCTCATCTTTCTGTCAGAAACGAACTGTAGGTTGAAACTGAGACTAGAATGCTGGATAAATTATGAGTTAAATGGTTTTGGTTGAAAAGCAGCTGTTTGCTTACGAGTGATTCTTTGACTTTTATACCACAATCTTAAAACAAGAAAAGGATATGTTTGTGACCTCTGTACTCCAGGAGTAGCACAAGTGACTAGGTCACTATAGGCTGAACTGATTCACGTTTGGTTTGGATTTTGCTGTTTTTCATTTTTAGTTTAGAAAATTCTTTGCTTAGGTTTCTTTCTCTCTCTCAATTGAAAAAACAACTTTTCAA

Coding sequence (CDS)

AAATTGGTTAAACCTCAAGCAGCCGGATCGAGTTCGTCTGAACGCTGCAGACTAATGACCTACGCTGCGGATTCAAGATGGTCGACGGCCGCCATGAGCGTCACGCTGGCGTTTATCTCTGCCTCCGCCATCATATCTCTTTATCTTGTGACAAAGAAGTCCAGAGAGTTGGGTACCAAGGTTCGACAGCTCGAGGCTTCTCTAGCAGCTTCATTAAAAAAAACCGCCGCCGAGAGACAAGGCCGAATACGGGCACAGCAGGCTTTAAGGAAAGCAGTTGCGGAACCCAAGTCTGATAGTGTGAAATGTACTTCATATCCCATGACGCCAATCGGTACTATCCAATCATCCTTCACTACCAGGAATGGGACTCCAAGGCAATCTTTGCTCGTACCTCTTGCAAGGGCACGGCTGGTGTTTTCTGCTCGCATTCCCCCAGCTTCCATTGAAGGTCTTGGAGAATATTCCCATTGCTGGGTCATATATGTCTTTCATTTGAACACAGATCTTGACAAGTTGTGGAAGGATCCATCGAAATCAAAATTCAAGGCCAAAGACAGCTCAATGGTAGGTTAG

Protein sequence

KLVKPQAAGSSSSERCRLMTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAERQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKDSSMVG
Homology
[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy Swiss-Prot
Match: Q9BU70 (tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 27/64 (42.19%), Postives = 40/64 (62.50%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           P+G ++S F+ +NGTPRQ  +   +RA L    RI   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 170 NTDL 171
           N  L
Sbjct: 92  NGHL 95

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy Swiss-Prot
Match: Q562D6 (tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 5.1e-06
Identity = 27/65 (41.54%), Postives = 38/65 (58.46%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           PIG ++S F  + GTPRQ  +   +RA L     I   P  S+ GL E+SH W+++VFH 
Sbjct: 32  PIGYLESCFPAKIGTPRQPSICSHSRACLKIRKNIFNNPEHSLMGLEEFSHVWILFVFHK 91

Query: 170 NTDLD 172
           N  L+
Sbjct: 92  NGHLN 96

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy Swiss-Prot
Match: Q4V7E0 (tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.7e-06
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 0 

Query: 110 PIGTIQSSFTTRNGTPRQSLLVPLARARLVFSARI---PPASIEGLGEYSHCWVIYVFHL 169
           PIG ++S F+ + GTPRQ  +   +RA L     I   P  S+ GL ++SH W+++VFH 
Sbjct: 32  PIGYLESCFSAKIGTPRQPSICSQSRACLKIRKSIFNNPEHSLMGLEQFSHVWILFVFHK 91

Query: 170 NTDLD 172
           N  L+
Sbjct: 92  NGHLN 96

[Close]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match: XP_004149885.1 (uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical protein Csa_019626 [Cucumis sativus])

HSP 1 Score: 315 bits (806), Expect = 2.23e-104
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match: XP_038879627.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 299 bits (766), Expect = 9.61e-100
Identity = 156/167 (93.41%), Postives = 165/167 (98.80%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match: XP_008453658.1 (PREDICTED: uncharacterized protein LOC103494312 isoform X2 [Cucumis melo])

HSP 1 Score: 300 bits (767), Expect = 7.97e-99
Identity = 160/173 (92.49%), Postives = 165/173 (95.38%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKDSSMVG 191
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK  ++ G
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVEAVQG 173

[Close]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match: XP_038879626.1 (tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 299 bits (766), Expect = 3.02e-98
Identity = 156/167 (93.41%), Postives = 165/167 (98.80%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+SRWS AA+SVTLAFISASA+ISLY+VTKKSRELGTKVR+LEASLA+SLKKTAAE
Sbjct: 1   MAYAAESRWSMAALSVTLAFISASAVISLYVVTKKSRELGTKVRELEASLASSLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTI+SSF+TRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIRSSFSTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match: XP_008453657.1 (PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 298 bits (763), Expect = 8.60e-98
Identity = 159/167 (95.21%), Postives = 162/167 (97.01%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match: A0A0A0LTM6 (TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 PE=4 SV=1)

HSP 1 Score: 315 bits (806), Expect = 1.08e-104
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE
Sbjct: 1   MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match: A0A1S3BWW3 (uncharacterized protein LOC103494312 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 3.86e-99
Identity = 160/173 (92.49%), Postives = 165/173 (95.38%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKDSSMVG 191
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK  ++ G
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAKVEAVQG 173

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match: A0A5D3CC84 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold499G00580 PE=4 SV=1)

HSP 1 Score: 298 bits (763), Expect = 4.16e-98
Identity = 159/167 (95.21%), Postives = 162/167 (97.01%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match: A0A1S3BW92 (tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494312 PE=4 SV=1)

HSP 1 Score: 298 bits (763), Expect = 4.16e-98
Identity = 159/167 (95.21%), Postives = 162/167 (97.01%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M YAA+S WSTAAMSV L FISASAIISLYLVTKKSRELGTKV+QLEASLAASLKKTAAE
Sbjct: 1   MAYAAESIWSTAAMSVALTFISASAIISLYLVTKKSRELGTKVQQLEASLAASLKKTAAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKAVAEPKSDSV+CTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL
Sbjct: 61  RQGRIRAQQALRKAVAEPKSDSVECTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGL EYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK
Sbjct: 121 VFSARIPPASIEGLVEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 167

[Close]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match: A0A6J1BYL5 (uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006924 PE=4 SV=1)

HSP 1 Score: 287 bits (734), Expect = 1.03e-93
Identity = 146/167 (87.43%), Postives = 162/167 (97.01%), Query Frame = 0 

Query: 19  MTYAADSRWSTAAMSVTLAFISASAIISLYLVTKKSRELGTKVRQLEASLAASLKKTAAE 78
           M Y A+SRWSTAAMS+ LAFISASAI+SLYLVT+KSRELG+KVR+LEASLAASLKKT AE
Sbjct: 1   MAYGAESRWSTAAMSIILAFISASAIVSLYLVTRKSRELGSKVRELEASLAASLKKTTAE 60

Query: 79  RQGRIRAQQALRKAVAEPKSDSVKCTSYPMTPIGTIQSSFTTRNGTPRQSLLVPLARARL 138
           RQGRIRAQQALRKA+A+PKSD+V+CTSYPMTPIG IQS+F+TRNGTPRQSL+VPLARARL
Sbjct: 61  RQGRIRAQQALRKALADPKSDNVECTSYPMTPIGVIQSAFSTRNGTPRQSLVVPLARARL 120

Query: 139 VFSARIPPASIEGLGEYSHCWVIYVFHLNTDLDKLWKDPSKSKFKAK 185
           VFSARIPPASIEGLGEYSHCW+IYVFHLNTDLDKLWK+PSKS+FKAK
Sbjct: 121 VFSARIPPASIEGLGEYSHCWIIYVFHLNTDLDKLWKNPSKSRFKAK 167

The following BLAST results are available for this feature:
BLAST of Cucsat.G20249.T10 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9BU702.1e-0742.19tRNA (adenine(37)-N6)-methyltransferase OS=Homo sapiens OX=9606 GN=TRMO PE=1 SV=... [more]
Q562D65.1e-0641.54tRNA (adenine(37)-N6)-methyltransferase OS=Mus musculus OX=10090 GN=Trmo PE=2 SV... [more]
Q4V7E06.7e-0640.00tRNA (adenine(37)-N6)-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trmo PE... [more]
BLAST of Cucsat.G20249.T10 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004149885.12.23e-104100.00uncharacterized protein LOC101219357 [Cucumis sativus] >KGN65285.1 hypothetical ... [more]
XP_038879627.19.61e-10093.41tRNA (adenine(37)-N6)-methyltransferase isoform X2 [Benincasa hispida][more]
XP_008453658.17.97e-9992.49PREDICTED: uncharacterized protein LOC103494312 isoform X2 [Cucumis melo][more]
XP_038879626.13.02e-9893.41tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Benincasa hispida][more]
XP_008453657.18.60e-9895.21PREDICTED: tRNA (adenine(37)-N6)-methyltransferase isoform X1 [Cucumis melo][more]
BLAST of Cucsat.G20249.T10 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LTM61.08e-104100.00TsaA-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G295140 P... [more]
A0A1S3BWW33.86e-9992.49uncharacterized protein LOC103494312 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3CC844.16e-9895.21tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo var. makuwa O... [more]
A0A1S3BW924.16e-9895.21tRNA (Adenine(37)-N6)-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1BYL51.03e-9387.43uncharacterized protein LOC111006924 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036414YaeB, N-terminal domain superfamilyGENE3D2.40.30.70coord: 107..190
e-value: 2.8E-15
score: 58.3
IPR023370TrmO-like, N-terminal domainPFAMPF01980TrmOcoord: 123..173
e-value: 2.7E-9
score: 37.3
IPR023370TrmO-like, N-terminal domainPROSITEPS51668TSAA_2coord: 108..191
score: 26.388304
IPR040372YaeB-likePANTHERPTHR12818UNCHARACTERIZEDcoord: 26..185
IPR036413YaeB-like superfamilySUPERFAMILY118196YaeB-likecoord: 104..170

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G20249Cucsat.G20249gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T10.E1Cucsat.G20249.T10.E1exon
Cucsat.G20249.T10.E2Cucsat.G20249.T10.E2exon
Cucsat.G20249.T10.E3Cucsat.G20249.T10.E3exon
Cucsat.G20249.T10.E4Cucsat.G20249.T10.E4exon
Cucsat.G20249.T10.E5Cucsat.G20249.T10.E5exon
Cucsat.G20249.T10.E6Cucsat.G20249.T10.E6exon
Cucsat.G20249.T10.E7Cucsat.G20249.T10.E7exon
Cucsat.G20249.T10.E8Cucsat.G20249.T10.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T10.C5Cucsat.G20249.T10.C5CDS
Cucsat.G20249.T10.C4Cucsat.G20249.T10.C4CDS
Cucsat.G20249.T10.C3Cucsat.G20249.T10.C3CDS
Cucsat.G20249.T10.C2Cucsat.G20249.T10.C2CDS
Cucsat.G20249.T10.C1Cucsat.G20249.T10.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G20249.T10Cucsat.G20249.T10-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016430 tRNA (adenine-N6-)-methyltransferase activity