Lsi05G020020 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G020020
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionEthylene-responsive transcription factor
Locationchr05 : 27064544 .. 27066613 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGGTAGAGAAACCAAAAGATCCGCCGCAGTCTCCCGCTGCATTTCACATTCCCAGCCCATTAAATCTCCGGCACGGCGGTTCGTCGGAGTCCGGCAGCGGCCGTCCGGCCGGTGGGTGGCGGAAATAAAAGACTCTTCTCAAAGAGTCCGCCTCTGGTTGGGCACCTACGACACGCCGGAGGAAGCCGCCAGAGCCTACGACGAGGCCGCCATTGCTCTTCGCGGCGAAAACGCGAGGACCAATTTCGTGTTTCCGGTGACCGGCCGGTCAGCGGATGCGAAATTTTCCGACATCGGCGTGTTGAAAGCGAAATTGAGCAAGAATCTGCAGAGTATTATAGCGAGAACGAGCGAACAGAGCAAGTCGTTGAAAAACAGAGTGAGCGATCGGTTCACTTTTGGGAATATTTTCAATTTCAGAAGCTACCAATCGGCAACAATGGCGGATTTGACGGCCATTGATAAGGCGGCTGTACAGCCGAGCATAATCGTACCCCACCTAGAAACCGATCAGCCCGGTTCGTGGGATGGCAGCGACAGCGACGGATTCCGGCAACTGGGTTTCAGTTCCGATGGGTCGGAGGGCGCCGGAGATTGGTGGGTGGATCGAATGTTGGAAGATGATTATTGTAAGGAAGGTTTAAAGAGTCAGAGGTTTAGGGTCTCTTCTTCTGTTGTTGTACCTCCAACTTTTAGTGGGTCTCCTAGCTATGGTGAATGTTAGATTTTCTATAAGAAAATTAAATTGGGATTAAGTCAAACACGATCATAGCTCAACTAGTATAAGTTTGTACCGTCCATTTCGAAATCAAAAATTCAATTCCTACACTCTCCACATTATAACAAAAATTAATGAGATGATAGAAGTCTTTAAAACCATGTGTATAATATTTAAAGAGTAATTATAATGAATAGCAATTTTAGAGATAATAACTAAGTGTATAGCAACATTTTTAAAAAATTGCAAATATAGCAAAGTCTATGAATGATATATTTCTATCTTTAATAGACTCTTATGGTCTATCAGTGATAGACCACCTAGACCATAAAAGTCTATCAGCAATAGCAGACTATCATTGATAGACTTTGCTATATTTGCAATTTTTTATATAATAACATTTTGAATCTGGTTGCTATATTTGCAACTACCTCTTATTTAAATGAAATATCGAAAGATTTGCATATTACAAGTATTGTGCCCTTAATCTTCTTGTGATAGAAAATGAAAATATTGGCTTATAAGTAACATGATGGAAATGTTATAGAAATTGAGTATTTGATTCATATAAAAAAAAAATAATTGTATAATCAACTTTAGATTGATTGAGATTAATGGATTAATGAAACAGTGGTATAACTAATTACTAATAGTGAGAGTTTAATTTTGTTGATGATTTGCGAGTGCATGTAAAGTTAACTGAAATTCAAGGGGTAGTGTGAAGTTCTGCTTGCTATTTGTTATAAAAGAACACAAATTAATTAAGGTTAAAATTTGATTTTTAGTTTCGAAAAGTAATAATTTAGTTCTATAAACTTTAGTAGTTAACGATTTAGTACATATACATTTAAATTGATAACAATTTAGTTTTTTAAATTTAATACGTAACACTTTTATTTATGCAATTTACAATTTATAACGATTTAATTCGAAAAATATTATTAAAATTTAATGAAATGTACTACACAAATAGATGAATAAAATTGATCGATAATCAATTTTGTTTTTATAAATTATAAAAATTAAATCGTTACGTAATAAAGTAGACACTTAATTTTGAAGGAATTTTTCATAACAGAGACTAAATTGTCACAAATTTTAAAAACTAAATTGTTATCATTCAGTTTAAAGACTAAATTAATATAAATTTAAGAATACAGGAATTAAATTGTTAAGAATTGAATTGTTAATTCTATAATAATAAAAGAATAACTTTGTAGAAATATTATTTAATTTATGAAAGGAGTGAGTGCATTGAAGTTTCCAAATGACTTCTTCATAGACCATTTATGTAATGATGATGCCATAATGGTCTATTGTGTAGACTGCCCCGGTTATATGTAA

mRNA sequence

ATGGCTGCCGGTAGAGAAACCAAAAGATCCGCCGCAGTCTCCCGCTGCATTTCACATTCCCAGCCCATTAAATCTCCGGCACGGCGGTTCGTCGGAGTCCGGCAGCGGCCGTCCGGCCGGTGGGTGGCGGAAATAAAAGACTCTTCTCAAAGAGTCCGCCTCTGGTTGGGCACCTACGACACGCCGGAGGAAGCCGCCAGAGCCTACGACGAGGCCGCCATTGCTCTTCGCGGCGAAAACGCGAGGACCAATTTCGTGTTTCCGGTGACCGGCCGGTCAGCGGATGCGAAATTTTCCGACATCGGCGTGTTGAAAGCGAAATTGAGCAAGAATCTGCAGAGTATTATAGCGAGAACGAGCGAACAGAGCAAGTCGTTGAAAAACAGAGTGAGCGATCGGTTCACTTTTGGGAATATTTTCAATTTCAGAAGCTACCAATCGGCAACAATGGCGGATTTGACGGCCATTGATAAGGCGGCTGTACAGCCGAGCATAATCGTACCCCACCTAGAAACCGATCAGCCCGGTTCGTGGGATGGCAGCGACAGCGACGGATTCCGGCAACTGGGTTTCAGTTCCGATGGGTCGGAGGGCGCCGGAGATTGGTGGGTGGATCGAATGTTGGAAGATGATTATTACTGCCCCGGTTATATGTAA

Coding sequence (CDS)

ATGGCTGCCGGTAGAGAAACCAAAAGATCCGCCGCAGTCTCCCGCTGCATTTCACATTCCCAGCCCATTAAATCTCCGGCACGGCGGTTCGTCGGAGTCCGGCAGCGGCCGTCCGGCCGGTGGGTGGCGGAAATAAAAGACTCTTCTCAAAGAGTCCGCCTCTGGTTGGGCACCTACGACACGCCGGAGGAAGCCGCCAGAGCCTACGACGAGGCCGCCATTGCTCTTCGCGGCGAAAACGCGAGGACCAATTTCGTGTTTCCGGTGACCGGCCGGTCAGCGGATGCGAAATTTTCCGACATCGGCGTGTTGAAAGCGAAATTGAGCAAGAATCTGCAGAGTATTATAGCGAGAACGAGCGAACAGAGCAAGTCGTTGAAAAACAGAGTGAGCGATCGGTTCACTTTTGGGAATATTTTCAATTTCAGAAGCTACCAATCGGCAACAATGGCGGATTTGACGGCCATTGATAAGGCGGCTGTACAGCCGAGCATAATCGTACCCCACCTAGAAACCGATCAGCCCGGTTCGTGGGATGGCAGCGACAGCGACGGATTCCGGCAACTGGGTTTCAGTTCCGATGGGTCGGAGGGCGCCGGAGATTGGTGGGTGGATCGAATGTTGGAAGATGATTATTACTGCCCCGGTTATATGTAA

Protein sequence

MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTSEQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPGSWDGSDSDGFRQLGFSSDGSEGAGDWWVDRMLEDDYYCPGYM
Homology
[Close]
BLAST of Lsi05G020020 vs. Swiss-Prot
Match: RA211_ARATH (Ethylene-responsive transcription factor RAP2-11 OS=Arabidopsis thaliana GN=RAP2-11 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 9.0e-20
Identity = 44/62 (70.97%), Postives = 53/62 (85.48%), Query Frame = 1 

Query: 24 KSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGENART 83
          K    +FVGVRQRPSG+WVAEIKD++Q++R+WLGT++T EEAARAYDEAA  LRG N RT
Sbjct: 16 KGNKTKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRGSNTRT 75

Query: 84 NF 86
          NF
Sbjct: 76 NF 77

[Close]
BLAST of Lsi05G020020 vs. Swiss-Prot
Match: CRF1_ARATH (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana GN=CRF1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 41/79 (51.90%), Postives = 54/79 (68.35%), Query Frame = 1 

Query: 23  IKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGENAR 82
           + +  R+F GVRQRP G+W AEI+D S+RVR+WLGT+DT EEAA  YD AAI LRG NA 
Sbjct: 80  VTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAE 139

Query: 83  TNFVFPVTGRSADAKFSDI 102
            NF  P    + +   +++
Sbjct: 140 LNFPPPPVTENVEEASTEV 158

[Close]
BLAST of Lsi05G020020 vs. Swiss-Prot
Match: CRF2_ARATH (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana GN=CRF2 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 55/118 (46.61%), Postives = 72/118 (61.02%), Query Frame = 1 

Query: 5   RETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEE 64
           R  K  + VS  +S +    +  ++F GVRQRP G+W AEI+D  +RVRLWLGTY+T EE
Sbjct: 98  RAVKSESTVSPVVS-ATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYNTAEE 157

Query: 65  AARAYDEAAIALRGENARTNF-VFPVTGRSADA-KFSDIGVLKAKLSKNLQSIIARTS 121
           AA  YD AAI LRG +A TNF V P T     A   S +   K K +K+ +S+ A +S
Sbjct: 158 AAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPPPSPVKKKKKKNNKSKKSVTASSS 214

[Close]
BLAST of Lsi05G020020 vs. Swiss-Prot
Match: ERF55_ARATH (Ethylene-responsive transcription factor ERF055 OS=Arabidopsis thaliana GN=ERF055 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 7.9e-16
Identity = 46/104 (44.23%), Postives = 63/104 (60.58%), Query Frame = 1 

Query: 20  SQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGE 79
           S  +  PA+ + GVRQR  G+WVAEI+    R RLWLGT+DT EEAA AYD AA  LRG+
Sbjct: 133 SGSVSKPAKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDRAAFKLRGD 192

Query: 80  NARTNF-VFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTSEQ 123
           +AR NF        S+ +   + G ++A +   L++I+A    Q
Sbjct: 193 SARLNFPALRYQTGSSPSDTGEYGPIQAAVDAKLEAILAEPKNQ 236

[Close]
BLAST of Lsi05G020020 vs. Swiss-Prot
Match: RAP24_ARATH (Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana GN=RAP2-4 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 52/126 (41.27%), Postives = 71/126 (56.35%), Query Frame = 1 

Query: 8   KRSAAVSRCISHSQPIKS-PARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAA 67
           K+S     C ++   + S P + + GVRQR  G+WVAEI+    R RLWLGT+DT EEAA
Sbjct: 129 KQSGVAGSCFAYGSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAA 188

Query: 68  RAYDEAAIALRGENARTNFV-FPVTGRSADAKFSDIGVLKAKLSKNLQSI---IARTSEQ 127
            AYD+AA  LRG+ AR NF      G      F +   L + +   L++I   +A T +Q
Sbjct: 189 LAYDKAAYKLRGDFARLNFPNLRHNGSHIGGDFGEYKPLHSSVDAKLEAICKSMAETQKQ 248

Query: 128 SKSLKN 129
            KS K+
Sbjct: 249 DKSTKS 254

[Close]
BLAST of Lsi05G020020 vs. TrEMBL
Match: A0A0A0L8Z9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124760 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 2.2e-102
Identity = 195/218 (89.45%), Postives = 201/218 (92.20%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MAAGRETKRSA VSRCISHSQP KSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 1   MAAGRETKRSATVSRCISHSQPNKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60

Query: 61  TPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTS 120
           TPEEAARAYDEAAIALRGENARTNFV P TG S D+K S+IGVLKAKLSKNLQSIIARTS
Sbjct: 61  TPEEAARAYDEAAIALRGENARTNFVSPATGWSPDSKVSNIGVLKAKLSKNLQSIIARTS 120

Query: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPGSWDG 180
           EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPH+ETD+ GSWD 
Sbjct: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHVETDRSGSWDS 180

Query: 181 SDSDGFRQLGFSSDGSEGAGDWWVDRMLEDDYYCPGYM 219
           SD +G R LGFSSDGSE AGDWWVDR+LEDD Y  G M
Sbjct: 181 SDGEGIRPLGFSSDGSEVAGDWWVDRILEDDCYNEGLM 218

[Close]
BLAST of Lsi05G020020 vs. TrEMBL
Match: B9IC72_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s07280g PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 8.4e-49
Identity = 124/203 (61.08%), Postives = 141/203 (69.46%), Query Frame = 1 

Query: 21  QPIKSPA-RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGE 80
           Q  K P  RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAA ALRGE
Sbjct: 19  QATKGPTLRRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAARALRGE 78

Query: 81  NARTNF--VFPVTGRSADAKFSDIGV---------------LKAKLSKNLQSIIARTSEQ 140
           NARTNF  V P   +S  +  S+ G+               LKAKLSKNLQSI+ART+E 
Sbjct: 79  NARTNFASVSPNLNQSGSSPSSNGGLNMSESDERHGLSFSSLKAKLSKNLQSIMARTTE- 138

Query: 141 SKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPG-SWDGS 198
           +KS KNRVSD FTF NIF+FR +Q     D+ +I+K  VQPSIIVPH+   +P  SW+ S
Sbjct: 139 NKSTKNRVSDHFTFANIFHFRGHQYQNPVDMKSIEK-VVQPSIIVPHVSDHKPSYSWETS 198

[Close]
BLAST of Lsi05G020020 vs. TrEMBL
Match: A0A067H4Y1_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g037119mg PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.4e-48
Identity = 130/231 (56.28%), Postives = 142/231 (61.47%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MAAG ET ++   +         K  ARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 1   MAAGSETSKNPTTTNSKGGQATTKPAARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60

Query: 61  TPEEAARAYDEAAIALRGENARTNFV-FPVTGRSADAKFSDIG----------------- 120
           TPEEAARAYDEAA ALRGENARTNF    V   +     SD G                 
Sbjct: 61  TPEEAARAYDEAARALRGENARTNFASSAVNNNNNPLNHSDSGNSSPCVNGRFVAQSDGR 120

Query: 121 ------VLKAKLSKNLQSIIARTSEQS-KSLKNRVSDRFTFGNIFNFRSYQSATMADLTA 180
                  LKAKLSKNLQSI+ARTSE + KS K+RVSD FTF +IF+FRSYQ  T  DL  
Sbjct: 121 NGLSFSSLKAKLSKNLQSIMARTSENNKKSTKSRVSDHFTFASIFHFRSYQYQTPVDLKN 180

Query: 181 IDKAAVQPSIIVPHL--ETDQPGSW-------DGSDSD--GFRQLGFSSDG 196
           I+K  VQPSIIVP        P SW       DGS ++  GFRQ G  SDG
Sbjct: 181 IEK-VVQPSIIVPQAADHMHDPSSWESSSTVSDGSSTEWGGFRQHGLDSDG 230

[Close]
BLAST of Lsi05G020020 vs. TrEMBL
Match: A5BB59_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011417 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 7.1e-48
Identity = 129/220 (58.64%), Postives = 149/220 (67.73%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MA+ + T  SAA S   + +QP K   RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 75  MASTKTT--SAATSTTNTMAQPSKPAVRRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 134

Query: 61  TPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFS----------------DIGVL 120
           TPEEAARAYDEAA ALRGENARTNF  PV   S  +  S                    L
Sbjct: 135 TPEEAARAYDEAARALRGENARTNFA-PVNPNSYQSGSSPSKGGFVSESEERNGLSFSSL 194

Query: 121 KAKLSKNLQSIIARTSEQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPS 180
           KAKLSKNLQSI+ARTS+ +KS K+RVSD FTF +IF+ RSY   +  D+ +++K  VQPS
Sbjct: 195 KAKLSKNLQSIMARTSD-NKSSKSRVSDHFTFASIFHSRSYHYQSPVDMKSMEK-VVQPS 254

Query: 181 IIVPHLETDQPGSWDGSD-SD------GFRQLGFSSDGSE 198
           IIVPH+  D+P SW+ S  SD      GFRQ G  SDGS+
Sbjct: 255 IIVPHV-ADEP-SWESSSVSDCSTEWVGFRQHGLDSDGSD 287

[Close]
BLAST of Lsi05G020020 vs. TrEMBL
Match: A0A061DTN1_THECC (AP2/ERF domain-containing transcription factor OS=Theobroma cacao GN=TCM_005425 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-47
Identity = 129/223 (57.85%), Postives = 145/223 (65.02%), Query Frame = 1 

Query: 3   AGRETKRSAAVSRCISH---SQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTY 62
           AG ET ++       +    SQP K   RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTY
Sbjct: 2   AGSETAKATTTGITTTDNTMSQPTKPMVRRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTY 61

Query: 63  DTPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFS----------------DIGV 122
           DTPEEAARAYDEAA ALRGENARTNF  PV   S+ +  S                    
Sbjct: 62  DTPEEAARAYDEAARALRGENARTNFA-PVNHNSSQSGSSPSNGGFVSQSDGRHGLSFSS 121

Query: 123 LKAKLSKNLQSIIARTSEQSKSLKNRVSDRFTFGNIFNFRSYQ-SATMADLTAIDKAAVQ 182
           LKAKLSKNLQSI+ART+E +KS K+RVSD FTF +IF+FR+YQ      D+  I+K  VQ
Sbjct: 122 LKAKLSKNLQSIMARTTE-NKSTKSRVSDHFTFASIFHFRNYQYQNPPVDMKNIEK-VVQ 181

Query: 183 PSIIVPHLETDQPGS-WDGSD-SD------GFRQLGFSSDGSE 198
           PSIIVP    + P S WD S  SD      GFRQ GF SDGS+
Sbjct: 182 PSIIVPRAVDEPPSSNWDSSSVSDCSNEWVGFRQHGFDSDGSD 221

[Close]
BLAST of Lsi05G020020 vs. TAIR10
Match: AT5G19790.1 (AT5G19790.1 related to AP2 11)

HSP 1 Score: 98.6 bits (244), Expect = 5.1e-21
Identity = 44/62 (70.97%), Postives = 53/62 (85.48%), Query Frame = 1 

Query: 24 KSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGENART 83
          K    +FVGVRQRPSG+WVAEIKD++Q++R+WLGT++T EEAARAYDEAA  LRG N RT
Sbjct: 16 KGNKTKFVGVRQRPSGKWVAEIKDTTQKIRMWLGTFETAEEAARAYDEAACLLRGSNTRT 75

Query: 84 NF 86
          NF
Sbjct: 76 NF 77

[Close]
BLAST of Lsi05G020020 vs. TAIR10
Match: AT4G11140.1 (AT4G11140.1 cytokinin response factor 1)

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-17
Identity = 41/79 (51.90%), Postives = 54/79 (68.35%), Query Frame = 1 

Query: 23  IKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGENAR 82
           + +  R+F GVRQRP G+W AEI+D S+RVR+WLGT+DT EEAA  YD AAI LRG NA 
Sbjct: 80  VTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAE 139

Query: 83  TNFVFPVTGRSADAKFSDI 102
            NF  P    + +   +++
Sbjct: 140 LNFPPPPVTENVEEASTEV 158

[Close]
BLAST of Lsi05G020020 vs. TAIR10
Match: AT4G23750.1 (AT4G23750.1 cytokinin response factor 2)

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-17
Identity = 55/118 (46.61%), Postives = 72/118 (61.02%), Query Frame = 1 

Query: 5   RETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEE 64
           R  K  + VS  +S +    +  ++F GVRQRP G+W AEI+D  +RVRLWLGTY+T EE
Sbjct: 98  RAVKSESTVSPVVS-ATTTTTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYNTAEE 157

Query: 65  AARAYDEAAIALRGENARTNF-VFPVTGRSADA-KFSDIGVLKAKLSKNLQSIIARTS 121
           AA  YD AAI LRG +A TNF V P T     A   S +   K K +K+ +S+ A +S
Sbjct: 158 AAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPPPSPVKKKKKKNNKSKKSVTASSS 214

[Close]
BLAST of Lsi05G020020 vs. TAIR10
Match: AT1G36060.1 (AT1G36060.1 Integrase-type DNA-binding superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 4.4e-17
Identity = 46/104 (44.23%), Postives = 63/104 (60.58%), Query Frame = 1 

Query: 20  SQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGE 79
           S  +  PA+ + GVRQR  G+WVAEI+    R RLWLGT+DT EEAA AYD AA  LRG+
Sbjct: 133 SGSVSKPAKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDRAAFKLRGD 192

Query: 80  NARTNF-VFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTSEQ 123
           +AR NF        S+ +   + G ++A +   L++I+A    Q
Sbjct: 193 SARLNFPALRYQTGSSPSDTGEYGPIQAAVDAKLEAILAEPKNQ 236

[Close]
BLAST of Lsi05G020020 vs. TAIR10
Match: AT1G78080.1 (AT1G78080.1 related to AP2 4)

HSP 1 Score: 84.7 bits (208), Expect = 7.5e-17
Identity = 52/126 (41.27%), Postives = 71/126 (56.35%), Query Frame = 1 

Query: 8   KRSAAVSRCISHSQPIKS-PARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAA 67
           K+S     C ++   + S P + + GVRQR  G+WVAEI+    R RLWLGT+DT EEAA
Sbjct: 129 KQSGVAGSCFAYGSGVPSKPTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAA 188

Query: 68  RAYDEAAIALRGENARTNFV-FPVTGRSADAKFSDIGVLKAKLSKNLQSI---IARTSEQ 127
            AYD+AA  LRG+ AR NF      G      F +   L + +   L++I   +A T +Q
Sbjct: 189 LAYDKAAYKLRGDFARLNFPNLRHNGSHIGGDFGEYKPLHSSVDAKLEAICKSMAETQKQ 248

Query: 128 SKSLKN 129
            KS K+
Sbjct: 249 DKSTKS 254

[Close]
BLAST of Lsi05G020020 vs. NCBI nr
Match: gi|449433103|ref|XP_004134337.1| (PREDICTED: ethylene-responsive transcription factor ERF071-like [Cucumis sativus])

HSP 1 Score: 379.8 bits (974), Expect = 3.2e-102
Identity = 195/218 (89.45%), Postives = 201/218 (92.20%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MAAGRETKRSA VSRCISHSQP KSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 1   MAAGRETKRSATVSRCISHSQPNKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60

Query: 61  TPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTS 120
           TPEEAARAYDEAAIALRGENARTNFV P TG S D+K S+IGVLKAKLSKNLQSIIARTS
Sbjct: 61  TPEEAARAYDEAAIALRGENARTNFVSPATGWSPDSKVSNIGVLKAKLSKNLQSIIARTS 120

Query: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPGSWDG 180
           EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPH+ETD+ GSWD 
Sbjct: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHVETDRSGSWDS 180

Query: 181 SDSDGFRQLGFSSDGSEGAGDWWVDRMLEDDYYCPGYM 219
           SD +G R LGFSSDGSE AGDWWVDR+LEDD Y  G M
Sbjct: 181 SDGEGIRPLGFSSDGSEVAGDWWVDRILEDDCYNEGLM 218

[Close]
BLAST of Lsi05G020020 vs. NCBI nr
Match: gi|659075362|ref|XP_008438105.1| (PREDICTED: ethylene-responsive transcription factor ERF071-like [Cucumis melo])

HSP 1 Score: 379.4 bits (973), Expect = 4.2e-102
Identity = 195/218 (89.45%), Postives = 199/218 (91.28%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MAAGRETKRSA VSRCISHSQP KSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 1   MAAGRETKRSATVSRCISHSQPNKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60

Query: 61  TPEEAARAYDEAAIALRGENARTNFVFPVTGRSADAKFSDIGVLKAKLSKNLQSIIARTS 120
           TPEEAARAYDEAAIALRGENARTNFV P  G S D+K S+IGVLKAKLSKNLQSIIARTS
Sbjct: 61  TPEEAARAYDEAAIALRGENARTNFVSPAAGWSPDSKVSNIGVLKAKLSKNLQSIIARTS 120

Query: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPGSWDG 180
           EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLT IDKAAVQPSIIVPH+ETDQ GSWD 
Sbjct: 121 EQSKSLKNRVSDRFTFGNIFNFRSYQSATMADLTTIDKAAVQPSIIVPHVETDQSGSWDS 180

Query: 181 SDSDGFRQLGFSSDGSEGAGDWWVDRMLEDDYYCPGYM 219
           SD DGFR LGF SDGSE AGDWWVDR+LEDD Y  G M
Sbjct: 181 SDGDGFRPLGFCSDGSEVAGDWWVDRILEDDDYNEGLM 218

[Close]
BLAST of Lsi05G020020 vs. NCBI nr
Match: gi|224130152|ref|XP_002320765.1| (hypothetical protein POPTR_0014s07280g [Populus trichocarpa])

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-48
Identity = 124/203 (61.08%), Postives = 141/203 (69.46%), Query Frame = 1 

Query: 21  QPIKSPA-RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGE 80
           Q  K P  RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAA ALRGE
Sbjct: 19  QATKGPTLRRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAARALRGE 78

Query: 81  NARTNF--VFPVTGRSADAKFSDIGV---------------LKAKLSKNLQSIIARTSEQ 140
           NARTNF  V P   +S  +  S+ G+               LKAKLSKNLQSI+ART+E 
Sbjct: 79  NARTNFASVSPNLNQSGSSPSSNGGLNMSESDERHGLSFSSLKAKLSKNLQSIMARTTE- 138

Query: 141 SKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPG-SWDGS 198
           +KS KNRVSD FTF NIF+FR +Q     D+ +I+K  VQPSIIVPH+   +P  SW+ S
Sbjct: 139 NKSTKNRVSDHFTFANIFHFRGHQYQNPVDMKSIEK-VVQPSIIVPHVSDHKPSYSWETS 198

[Close]
BLAST of Lsi05G020020 vs. NCBI nr
Match: gi|641867306|gb|KDO85990.1| (hypothetical protein CISIN_1g037119mg, partial [Citrus sinensis])

HSP 1 Score: 200.3 bits (508), Expect = 3.5e-48
Identity = 130/231 (56.28%), Postives = 142/231 (61.47%), Query Frame = 1 

Query: 1   MAAGRETKRSAAVSRCISHSQPIKSPARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60
           MAAG ET ++   +         K  ARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD
Sbjct: 1   MAAGSETSKNPTTTNSKGGQATTKPAARRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYD 60

Query: 61  TPEEAARAYDEAAIALRGENARTNFV-FPVTGRSADAKFSDIG----------------- 120
           TPEEAARAYDEAA ALRGENARTNF    V   +     SD G                 
Sbjct: 61  TPEEAARAYDEAARALRGENARTNFASSAVNNNNNPLNHSDSGNSSPCVNGRFVAQSDGR 120

Query: 121 ------VLKAKLSKNLQSIIARTSEQS-KSLKNRVSDRFTFGNIFNFRSYQSATMADLTA 180
                  LKAKLSKNLQSI+ARTSE + KS K+RVSD FTF +IF+FRSYQ  T  DL  
Sbjct: 121 NGLSFSSLKAKLSKNLQSIMARTSENNKKSTKSRVSDHFTFASIFHFRSYQYQTPVDLKN 180

Query: 181 IDKAAVQPSIIVPHL--ETDQPGSW-------DGSDSD--GFRQLGFSSDG 196
           I+K  VQPSIIVP        P SW       DGS ++  GFRQ G  SDG
Sbjct: 181 IEK-VVQPSIIVPQAADHMHDPSSWESSSTVSDGSSTEWGGFRQHGLDSDG 230

[Close]
BLAST of Lsi05G020020 vs. NCBI nr
Match: gi|743789146|ref|XP_011035387.1| (PREDICTED: uncharacterized protein LOC105133206 [Populus euphratica])

HSP 1 Score: 200.3 bits (508), Expect = 3.5e-48
Identity = 123/203 (60.59%), Postives = 141/203 (69.46%), Query Frame = 1 

Query: 21  QPIKSPA-RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAAIALRGE 80
           Q  K P  RRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAA ALRGE
Sbjct: 19  QATKGPTVRRFVGVRQRPSGRWVAEIKDSSQRVRLWLGTYDTPEEAARAYDEAARALRGE 78

Query: 81  NARTNF--VFPVTGRSADAKFSDIGV---------------LKAKLSKNLQSIIARTSEQ 140
           NARTNF  V P   +   +  S+ G+               LKAKLSKNLQSI+ART+E 
Sbjct: 79  NARTNFASVNPNLNQYGSSPSSNGGLKMSESDGRHGLSFSSLKAKLSKNLQSIMARTTE- 138

Query: 141 SKSLKNRVSDRFTFGNIFNFRSYQSATMADLTAIDKAAVQPSIIVPHLETDQPG-SWDGS 198
           +K+ KNRVSD FTF NIF+FRS+Q     D+ +I+K  VQPSIIVPH+   +P  SW+ S
Sbjct: 139 NKATKNRVSDHFTFANIFHFRSHQYQNPVDMKSIEK-VVQPSIIVPHVSDHKPSYSWETS 198

The following BLAST results are available for this feature:
BLAST of Lsi05G020020 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RA211_ARATH9.0e-2070.97Ethylene-responsive transcription factor RAP2-11 OS=Arabidopsis thaliana GN=RAP2... [more]
CRF1_ARATH2.1e-1651.90Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana GN=CRF1 PE... [more]
CRF2_ARATH2.1e-1646.61Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana GN=CRF2 PE... [more]
ERF55_ARATH7.9e-1644.23Ethylene-responsive transcription factor ERF055 OS=Arabidopsis thaliana GN=ERF05... [more]
RAP24_ARATH1.3e-1541.27Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana GN=RAP2-... [more]
BLAST of Lsi05G020020 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8Z9_CUCSA2.2e-10289.45Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124760 PE=4 SV=1[more]
B9IC72_POPTR8.4e-4961.08Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s07280g PE=4 SV=1[more]
A0A067H4Y1_CITSI2.4e-4856.28Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g037119mg PE=4 S... [more]
A5BB59_VITVI7.1e-4858.64Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011417 PE=4 SV=1[more]
A0A061DTN1_THECC2.1e-4757.85AP2/ERF domain-containing transcription factor OS=Theobroma cacao GN=TCM_005425 ... [more]
BLAST of Lsi05G020020 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G19790.15.1e-2170.97 related to AP2 11[more]
AT4G11140.11.2e-1751.90 cytokinin response factor 1[more]
AT4G23750.11.2e-1746.61 cytokinin response factor 2[more]
AT1G36060.14.4e-1744.23 Integrase-type DNA-binding superfamily protein[more]
AT1G78080.17.5e-1741.27 related to AP2 4[more]
BLAST of Lsi05G020020 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449433103|ref|XP_004134337.1|3.2e-10289.45PREDICTED: ethylene-responsive transcription factor ERF071-like [Cucumis sativus... [more]
gi|659075362|ref|XP_008438105.1|4.2e-10289.45PREDICTED: ethylene-responsive transcription factor ERF071-like [Cucumis melo][more]
gi|224130152|ref|XP_002320765.1|1.2e-4861.08hypothetical protein POPTR_0014s07280g [Populus trichocarpa][more]
gi|641867306|gb|KDO85990.1|3.5e-4856.28hypothetical protein CISIN_1g037119mg, partial [Citrus sinensis][more]
gi|743789146|ref|XP_011035387.1|3.5e-4860.59PREDICTED: uncharacterized protein LOC105133206 [Populus euphratica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003677DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: INTERPRO
TermDefinition
IPR017392AP2/ERF-transcript_factor
IPR016177DNA-bd_dom_sf
IPR001471AP2/ERF_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G020020.1Lsi05G020020.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 52..68
score: 1.8E-7coord: 30..41
score: 1.
IPR001471AP2/ERF domainGENE3DG3DSA:3.30.730.10coord: 29..85
score: 2.7
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 29..79
score: 1.8
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 29..92
score: 8.4
IPR001471AP2/ERF domainPROFILEPS51032AP2_ERFcoord: 29..86
score: 20
IPR016177DNA-binding domainunknownSSF54171DNA-binding domaincoord: 30..86
score: 2.55
IPR017392AP2/ERF transcription factor ERF/PTI6PANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 27..213
score: 1.3
NoneNo IPR availablePANTHERPTHR31194:SF7SUBFAMILY NOT NAMEDcoord: 27..213
score: 1.3

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi05G020020Csa3G124760Cucumber (Chinese Long) v2culsiB209
Lsi05G020020CSPI03G09170Wild cucumber (PI 183967)cpilsiB207
Lsi05G020020Cucsa.258020Cucumber (Gy14) v1cgylsiB415
Lsi05G020020CmaCh14G020050Cucurbita maxima (Rimu)cmalsiB245
Lsi05G020020CmoCh14G020660Cucurbita moschata (Rifu)cmolsiB233
Lsi05G020020Cla021270Watermelon (97103) v1lsiwmB365
Lsi05G020020MELO3C006149Melon (DHL92) v3.5.1lsimeB378
Lsi05G020020ClCG05G001890Watermelon (Charleston Gray)lsiwcgB335
Lsi05G020020MELO3C006149.2Melon (DHL92) v3.6.1lsimedB396
Lsi05G020020Cla97C05G082330Watermelon (97103) v2lsiwmbB333
Lsi05G020020Bhi01G000228Wax gourdlsiwgoB435
Lsi05G020020Cp4.1LG03g17040Cucurbita pepo (Zucchini)cpelsiB506
Lsi05G020020CsGy3G009250Cucumber (Gy14) v2cgyblsiB196
Lsi05G020020Carg14808Silver-seed gourdcarlsiB565
Lsi05G020020CsaV3_3G009290Cucumber (Chinese Long) v3cuclsiB228
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi05G020020Cucumber (Chinese Long) v3cuclsiB227
Lsi05G020020Cucumber (Chinese Long) v3cuclsiB491
Lsi05G020020Watermelon (97103) v2lsiwmbB345
Lsi05G020020Bottle gourd (USVL1VR-Ls)lsilsiB007
Lsi05G020020Bottle gourd (USVL1VR-Ls)lsilsiB160
Lsi05G020020Cucumber (Gy14) v1cgylsiB585
Lsi05G020020Cucurbita maxima (Rimu)cmalsiB583
Lsi05G020020Cucurbita maxima (Rimu)cmalsiB764
Lsi05G020020Cucurbita moschata (Rifu)cmolsiB557
Lsi05G020020Cucurbita moschata (Rifu)cmolsiB757
Lsi05G020020Cucurbita pepo (Zucchini)cpelsiB271
Lsi05G020020Cucurbita pepo (Zucchini)cpelsiB714
Lsi05G020020Wild cucumber (PI 183967)cpilsiB205
Lsi05G020020Wild cucumber (PI 183967)cpilsiB470
Lsi05G020020Cucumber (Chinese Long) v2culsiB462
Lsi05G020020Melon (DHL92) v3.5.1lsimeB375
Lsi05G020020Watermelon (Charleston Gray)lsiwcgB334
Lsi05G020020Watermelon (Charleston Gray)lsiwcgB346
Lsi05G020020Watermelon (97103) v1lsiwmB364
Lsi05G020020Watermelon (97103) v1lsiwmB385
Lsi05G020020Cucumber (Gy14) v2cgyblsiB430
Lsi05G020020Silver-seed gourdcarlsiB442