Lsi05G014780 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G014780
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	tRNA/rRNA methyltransferase family protein
Location	chr05 : 22599199 .. 22601238 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATCGAAAGCTACATTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCTTGGCCCGAAGCGCCACGGCCTTCGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACTCCTTTGGTAGCCATGGTTCCACTTCTCATCTCCGCTTCCGCCATTTTCACTCCCTTCTCGACGCCCAAACTTTCCTCAAGGTTCTGCTCTCTTTCTTTCTTCCCTTACGTATTCATTTGGTTATGGGTTTGTTTTGAAATTTGAATTGCGTTCTTCGGTTCTGTAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTAGGAAACGAGGTTGGTTGTTCGTTTTCATTTTATTGGGTTGTCATTGATCGTGATATCTATATGCTTGTATTGAAATTCCCTTGTTTGATCTGCCCTTGATCTATTTCCAGGGAATTTCTGTACGCTGTAACTCATAATGTTGAATTGATAGGGTTGTTTTCTATATGCTGTTCCTTTCTAATTCAATTGATACTTATGACTTAAGAACTTTTTGTCTCCTTGAGTTGATCTTGAGGTGGGCAAAACATCCTTGAGACTGGTGGGTTGCCCATGAGTTTAATAATGCAAGGAATGAGCTCTGATAGCATGGTAAAAAATGGTACGAACTTACTTGAACAGGATTTGTTCTATAGGTTGTACACCTATGAGTTCTAAGTATGGTGAAAGATGATAACACCGGGCTTAATGTTTCTCTAGGATTAATATTTGTTGTAATGCTACTTAATGTTTCTCTAGCAATAATATTGCATTTTTTTCCTATAAAAAATACAATTTTTTCCACCTTTGATATGATGAGATTAGGATTTAATATGGCATCATAGATCTCTTGTTTTATTGTAGGATCCACGTCTCTATAATGATTTCGGCATTGAGAGTAAGTTAGTGGCATGGTCATCTGTCATCTGGATGGAATTGGATTAGAATCATTTGGAAATAATTTTGACACTATTTTCAGCATTGAATCACAGTCACCCATGGTTATATGTTTGTTGTTTTCAGGGCACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTTGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGGTAATTGATTCAGACATTTGCATTTTTATCTCCCCCTTTTGAGTTTTTTTTTTTTTTTGCTAACATGTCTAATGTATATCACCTGTAAGAATTATGAGTCTCTCTTGATATTGCTCATTTATACTTTTGGTTTTCGACCAAGCAAAACTGTGAGTACTGTAAAAGTATGCAATCTATGAAAGAACTATAGGAAGAAATGCACATATATCTTAGAAGTACGCAAGGAGCGGCAATGATCTCATCTAGTTATTTTTACCTGTTTATCTGAGTCTTGAATCAATTCTTCAGAAATGATCCCTTAAAGCTGAAACTACGGAAACTGCGTCTTACGAATAACTATGGCTTGGCTGAATTGTATAATGTGGCAGTTCGTAATAGTTCACTTACTTAATGTTGATGATGATGATCTAGGTAGATCGATAAATCATCCTCTCATTGTCAAGTATGCATCGGTTTTCTAGTGTGCATATATCGAGATTTAAGTGAAAATCGAGTTGGTTATGTCTTATTTGATTAAATCAATGGAAATGATACTTGTCACTTTTTGTAGATGTAAATCTAACTTTTCATTGATTGTATTTTTCAGTTTGGGCAGGATTTCCTGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGCCAAGCGAAATTATTGCCATGAAACAGAAGAATCTATTATTGAAGAGCGAAAGTCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGAATGATTGATGATTCAACATCCAACCTTCTTGATACATTGTTTATTGACCAATGA

mRNA sequence

ATGGGGATCGAAAGCTACATTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCTTGGCCCGAAGCGCCACGGCCTTCGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACTCCTTTGGTAGCCATGGTTCCACTTCTCATCTCCGCTTCCGCCATTTTCACTCCCTTCTCGACGCCCAAACTTTCCTCAAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTAGGAAACGAGGGCACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTTGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCTGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGCCAAGCGAAATTATTGCCATGAAACAGAAGAATCTATTATTGAAGAGCGAAAGTCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGAATGATTGATGATTCAACATCCAACCTTCTTGATACATTGTTTATTGACCAATGA

Coding sequence (CDS)

ATGGGGATCGAAAGCTACATTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCTTGGCCCGAAGCGCCACGGCCTTCGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACTCCTTTGGTAGCCATGGTTCCACTTCTCATCTCCGCTTCCGCCATTTTCACTCCCTTCTCGACGCCCAAACTTTCCTCAAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTAGGAAACGAGGGCACAGGGCTTTCTGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTTGGCACTGCTTCTTTGAATGTAACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCTGAAAGATGTCGCGATGGTAACAAGTTTATAGTGGCTGAAAGACCAGTAAAACAGGCCAAGCGAAATTATTGCCATGAAACAGAAGAATCTATTATTGAAGAGCGAAAGTCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGAATGATTGATGATTCAACATCCAACCTTCTTGATACATTGTTTATTGACCAATGA

Protein sequence

MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYIPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEESIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ

Homology

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BLAST of Lsi05G014780 vs. Swiss-Prot
Match: TRMHL_STAHJ (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=SH2477 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.4e-10
Identity = 44/146 (30.14%), Postives = 71/146 (48.63%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----SFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        +  S G+  H+   
Sbjct: 93  GLSTVVILDGLEDPHNLGSILRTADASGVDGVIIPKRRSVALTQTVAKASTGAIQHVPVM 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LKE    + G E  DNA            A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKEHGYWVAGAE-ADNATDYRDMAADMPLAIVIGSEGQGMSRLVKDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

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BLAST of Lsi05G014780 vs. Swiss-Prot
Match: TRMHL_STAAM (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=SAV0531 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----SFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        +  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Lsi05G014780 vs. Swiss-Prot
Match: TRMHL_STAAN (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain N315) GN=SA0490 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----SFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        +  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Lsi05G014780 vs. Swiss-Prot
Match: TRMHL_STAAR (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MRSA252) GN=SAR0535 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----SFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        +  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Lsi05G014780 vs. Swiss-Prot
Match: TRMHL_STAAS (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MSSA476) GN=SAS0489 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFN-----SFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        +  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

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BLAST of Lsi05G014780 vs. TrEMBL
Match: A0A0A0LAH1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150750 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 2.5e-117
Identity = 207/218 (94.95%), Postives = 215/218 (98.62%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MGIESY+VVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPV+Q KRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SI+EERKSRRENASKGFFDEGMID+S SNLLDTLFIDQ
Sbjct: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 218

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BLAST of Lsi05G014780 vs. TrEMBL
Match: A5AVL9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g02300 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 3.8e-102
Identity = 185/217 (85.25%), Postives = 196/217 (90.32%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           M +ESY+VVHNIAKRHNVGTLARSA+A GVSELILVGRRDFNSFGSHGSTSHLRFRHFHS
Sbjct: 1   MVVESYVVVHNIAKRHNVGTLARSASALGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLS KEC ICDFF+Y
Sbjct: 61  LLDARHFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSVKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDGNKF+VAERP +Q +RNYC ET +
Sbjct: 121 IPQYGGGTASLNVTVAASIVLHHFGVWAGFSERIRDGNKFVVAERPAQQVRRNYCTETAD 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFID 218
           SIIEERK RR+NAS GFFDE    +S SNLLD LF D
Sbjct: 181 SIIEERKIRRQNASNGFFDESGSGESPSNLLDALFGD 217

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BLAST of Lsi05G014780 vs. TrEMBL
Match: A0A067L6D1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02058 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.5e-101
Identity = 185/218 (84.86%), Postives = 198/218 (90.83%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MGIESYIVVHNIAKRHNVGTLARSATAFGV+ELILVGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LL+A+ FLKEKDCDICGVEI D A+ VN+HPF +STAFLLGNEGTGLSAKEC ICDFF+Y
Sbjct: 61  LLEARNFLKEKDCDICGVEIADGALPVNEHPFNKSTAFLLGNEGTGLSAKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER R+GNKFIVAERPVKQAK  YC ETEE
Sbjct: 121 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERIREGNKFIVAERPVKQAK--YCTETEE 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SIIEERK+R+E+AS GFFDE    DS SNLLD LF D+
Sbjct: 181 SIIEERKTRKEHASNGFFDESRNGDSASNLLDALFNDE 216

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BLAST of Lsi05G014780 vs. TrEMBL
Match: A0A161ZT46_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_022857 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-101
Identity = 180/213 (84.51%), Postives = 195/213 (91.55%), Query Frame = 1


Query: 3   IESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHSLL 62
           +E Y+VVHNIAKRHNVGTLARSATAFGV+ELILVGRRDFNSFGSHGSTSH RFRHFHSL 
Sbjct: 21  VECYVVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNSFGSHGSTSHCRFRHFHSLA 80

Query: 63  DAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYIP 122
           DA++FLKEKDCDICGVEITD+AVAVNQHPF +STAFLLGNEGTGLS+KECAICDFF+YIP
Sbjct: 81  DARSFLKEKDCDICGVEITDDAVAVNQHPFTKSTAFLLGNEGTGLSSKECAICDFFVYIP 140

Query: 123 QYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEESI 182
           QYG GTASLNVT+AASIVLHHFGVWAGF ER RDGNKF+VAERPVKQ+ RNYC ET E+I
Sbjct: 141 QYGCGTASLNVTIAASIVLHHFGVWAGFSERSRDGNKFVVAERPVKQSGRNYCTETAETI 200

Query: 183 IEERKSRRENASKGFFDEGMIDDSTSNLLDTLF 216
           IEERK R+ENA  GFFDE +  DS SNLLD LF
Sbjct: 201 IEERKLRKENALNGFFDESVKQDSPSNLLDALF 233

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BLAST of Lsi05G014780 vs. TrEMBL
Match: A0A061ENU9_THECC (OBP33pep like protein isoform 1 OS=Theobroma cacao GN=TCM_021087 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 2.7e-100
Identity = 176/218 (80.73%), Postives = 198/218 (90.83%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           M IESY+VVHNIAK+HNVGTLARSATAFGVSE+I+VGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MAIESYVVVHNIAKKHNVGTLARSATAFGVSEIIIVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           L DA  +LKEKDCDICGVEITD AV+V +HPFK+STAFLLGNEGTGLSAKEC +CDFF+Y
Sbjct: 61  LTDANLYLKEKDCDICGVEITDGAVSVTEHPFKKSTAFLLGNEGTGLSAKECEMCDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDGNKF+VAE+P+KQ  R YC ET++
Sbjct: 121 IPQYGGGTASLNVTVAASIVLHHFGVWAGFSERIRDGNKFVVAEKPLKQVSRKYCTETDD 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SIIEERKSRRENAS GFFDEG    S++NLLD++F ++
Sbjct: 181 SIIEERKSRRENASNGFFDEGKNGASSANLLDSVFTEE 218

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BLAST of Lsi05G014780 vs. TAIR10
Match: AT4G15520.1 (AT4G15520.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 347.1 bits (889), Expect = 8.2e-96
Identity = 168/220 (76.36%), Postives = 196/220 (89.09%), Query Frame = 1


Query: 2   GIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHSL 61
           G ESY+VVHNIAKRHNVGTLARSATAFGV+ELILVGRRDFN+FGSHGS SH+RFRHFHSL
Sbjct: 3   GFESYVVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSASHIRFRHFHSL 62

Query: 62  LDAQTFLK-EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 121
           ++A+ +LK EKDCDICGVEI D A AVN+HPFKR+TAFLLGNEG+GLSAKE  ICDFF+Y
Sbjct: 63  IEARNYLKEEKDCDICGVEIADGASAVNEHPFKRNTAFLLGNEGSGLSAKEYEICDFFVY 122

Query: 122 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 181
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDG+KFIVA+RPV+Q +RN+C  TEE
Sbjct: 123 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERVRDGSKFIVADRPVRQGRRNFCAGTEE 182

Query: 182 SIIEERKSRRENASKGFFDEGMIDD--STSNLLDTLFIDQ 219
           S+IEERK R+E+A  GFFD+   ++  S+S+LLD LF+++
Sbjct: 183 SVIEERKLRKESAENGFFDDNGNENGSSSSDLLDGLFLNE 222

[Close]

BLAST of Lsi05G014780 vs. TAIR10
Match: AT4G17610.1 (AT4G17610.1 tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 49.7 bits (117), Expect = 2.7e-06
Identity = 28/76 (36.84%), Postives = 42/76 (55.26%), Query Frame = 1


Query: 69   KEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYIPQYGVGT 128
            K +   I G+E T N+V+++++ F + T  +LG E  G+      I D  I IPQ GV  
Sbjct: 1770 KREGFSILGLEQTANSVSLDKYQFPKKTVLVLGREKEGIPVDIIHILDACIEIPQLGV-V 1829

Query: 129  ASLNVTVAASIVLHHF 145
             SLNV V+ +I L  +
Sbjct: 1830 RSLNVHVSGAIALWEY 1844

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BLAST of Lsi05G014780 vs. NCBI nr
Match: gi|659076582|ref|XP_008438758.1| (PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis melo])

HSP 1 Score: 435.6 bits (1119), Expect = 4.9e-119
Identity = 211/218 (96.79%), Postives = 216/218 (99.08%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MGIESY+VVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFN+FGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+Q KRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Lsi05G014780 vs. NCBI nr
Match: gi|449432771|ref|XP_004134172.1| (PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis sativus])

HSP 1 Score: 429.5 bits (1103), Expect = 3.5e-117
Identity = 207/218 (94.95%), Postives = 215/218 (98.62%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MGIESY+VVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPV+Q KRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SI+EERKSRRENASKGFFDEGMID+S SNLLDTLFIDQ
Sbjct: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 218

[Close]

BLAST of Lsi05G014780 vs. NCBI nr
Match: gi|225432460|ref|XP_002278797.1| (PREDICTED: uncharacterized protein LOC100252797 isoform X1 [Vitis vinifera])

HSP 1 Score: 379.0 bits (972), Expect = 5.5e-102
Identity = 185/217 (85.25%), Postives = 196/217 (90.32%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           M +ESY+VVHNIAKRHNVGTLARSA+A GVSELILVGRRDFNSFGSHGSTSHLRFRHFHS
Sbjct: 1   MVVESYVVVHNIAKRHNVGTLARSASALGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLS KEC ICDFF+Y
Sbjct: 61  LLDARHFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSVKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDGNKF+VAERP +Q +RNYC ET +
Sbjct: 121 IPQYGGGTASLNVTVAASIVLHHFGVWAGFSERIRDGNKFVVAERPAQQVRRNYCTETAD 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFID 218
           SIIEERK RR+NAS GFFDE    +S SNLLD LF D
Sbjct: 181 SIIEERKIRRQNASNGFFDESGSGESPSNLLDALFGD 217

[Close]

BLAST of Lsi05G014780 vs. NCBI nr
Match: gi|1012004119|ref|XP_015939488.1| (PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Arachis duranensis])

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-101
Identity = 180/217 (82.95%), Postives = 200/217 (92.17%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MG ESY+VVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGS++HLRFRHFHS
Sbjct: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSSAHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           L DA+ FLKEKD DICGVEIT+NA+ VNQHPFK+STAFLLGNEGTGLSAKEC ICDFF+Y
Sbjct: 61  LHDARQFLKEKDFDICGVEITENAMPVNQHPFKKSTAFLLGNEGTGLSAKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDGNKF+VAERPVKQ +RN+C ETE+
Sbjct: 121 IPQYGGGTASLNVTVAASIVLHHFGVWAGFGERSRDGNKFVVAERPVKQVRRNFCTETED 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFID 218
            I+EERK+RRENA+ GFFDE   ++S+SN+LD LF+D
Sbjct: 181 DIVEERKARRENAANGFFDENENNNSSSNMLDALFVD 217

[Close]

BLAST of Lsi05G014780 vs. NCBI nr
Match: gi|802578494|ref|XP_012069459.1| (PREDICTED: uncharacterized protein LOC105631867 [Jatropha curcas])

HSP 1 Score: 377.1 bits (967), Expect = 2.1e-101
Identity = 185/218 (84.86%), Postives = 198/218 (90.83%), Query Frame = 1


Query: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60
           MGIESYIVVHNIAKRHNVGTLARSATAFGV+ELILVGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYIVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LL+A+ FLKEKDCDICGVEI D A+ VN+HPF +STAFLLGNEGTGLSAKEC ICDFF+Y
Sbjct: 61  LLEARNFLKEKDCDICGVEIADGALPVNEHPFNKSTAFLLGNEGTGLSAKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQAKRNYCHETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER R+GNKFIVAERPVKQAK  YC ETEE
Sbjct: 121 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERIREGNKFIVAERPVKQAK--YCTETEE 180

Query: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 219
           SIIEERK+R+E+AS GFFDE    DS SNLLD LF D+
Sbjct: 181 SIIEERKTRKEHASNGFFDESRNGDSASNLLDALFNDE 216

The following BLAST results are available for this feature:

BLAST of Lsi05G014780 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRMHL_STAHJ	6.4e-10	30.14	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus ...	[more]
TRMHL_STAAM	1.1e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
TRMHL_STAAN	1.1e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
TRMHL_STAAR	1.1e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
TRMHL_STAAS	1.1e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]

BLAST of Lsi05G014780 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAH1_CUCSA	2.5e-117	94.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150750 PE=4 SV=1	[more]
A5AVL9_VITVI	3.8e-102	85.25	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g02300 PE=4 SV=...	[more]
A0A067L6D1_JATCU	1.5e-101	84.86	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02058 PE=4 SV=1	[more]
A0A161ZT46_DAUCA	3.2e-101	84.51	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_022857 PE=4 SV=1	[more]
A0A061ENU9_THECC	2.7e-100	80.73	OBP33pep like protein isoform 1 OS=Theobroma cacao GN=TCM_021087 PE=4 SV=1	[more]

BLAST of Lsi05G014780 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G15520.1	8.2e-96	76.36	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G17610.1	2.7e-06	36.84	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

BLAST of Lsi05G014780 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659076582\|ref\|XP_008438758.1\|	4.9e-119	96.79	PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis melo]	[more]
gi\|449432771\|ref\|XP_004134172.1\|	3.5e-117	94.95	PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis sativus]	[more]
gi\|225432460\|ref\|XP_002278797.1\|	5.5e-102	85.25	PREDICTED: uncharacterized protein LOC100252797 isoform X1 [Vitis vinifera]	[more]
gi\|1012004119\|ref\|XP_015939488.1\|	1.6e-101	82.95	PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Arachis duranensis]	[more]
gi\|802578494\|ref\|XP_012069459.1\|	2.1e-101	84.86	PREDICTED: uncharacterized protein LOC105631867 [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008173	RNA methyltransferase activity
GO:0003723	RNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006396	RNA processing

Vocabulary: **INTERPRO**
Term	Definition
IPR001537	SpoU_MeTrfase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000453	enzyme-directed rRNA 2'-O-methylation
biological_process	GO:0006635	fatty acid beta-oxidation
biological_process	GO:0016558	protein import into peroxisome matrix
biological_process	GO:0001510	RNA methylation
biological_process	GO:0006396	RNA processing
biological_process	GO:0009451	RNA modification
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0070039	rRNA (guanosine-2'-O-)-methyltransferase activity
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G014780.1	Lsi05G014780.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 6..142 score: 2.3
None	No IPR available	PANTHER	PTHR12029	RNA METHYLTRANSFERASE	coord: 6..169 score: 1.7
None	No IPR available	PANTHER	PTHR12029:SF48	OBP33PEP LIKE PROTEIN	coord: 6..169 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G014780	Csa3G150750	Cucumber (Chinese Long) v2	culsiB199
Lsi05G014780	CSPI03G13710	Wild cucumber (PI 183967)	cpilsiB198
Lsi05G014780	Cucsa.253780	Cucumber (Gy14) v1	cgylsiB420
Lsi05G014780	Cla021684	Watermelon (97103) v1	lsiwmB362
Lsi05G014780	MELO3C006586	Melon (DHL92) v3.5.1	lsimeB369
Lsi05G014780	ClCG05G005600	Watermelon (Charleston Gray)	lsiwcgB333
Lsi05G014780	MELO3C006586.2	Melon (DHL92) v3.6.1	lsimedB385
Lsi05G014780	Cla97C05G087000	Watermelon (97103) v2	lsiwmbB330
Lsi05G014780	Bhi01G000653	Wax gourd	lsiwgoB432
Lsi05G014780	Cp4.1LG08g04360	Cucurbita pepo (Zucchini)	cpelsiB718
Lsi05G014780	CsGy3G013860	Cucumber (Gy14) v2	cgyblsiB184
Lsi05G014780	Carg17385	Silver-seed gourd	carlsiB552
Lsi05G014780	Carg20156	Silver-seed gourd	carlsiB761
Lsi05G014780	CsaV3_3G013940	Cucumber (Chinese Long) v3	cuclsiB218

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G014780	Watermelon (97103) v2	lsiwmbB344
Lsi05G014780	Wax gourd	lsiwgoB422
Lsi05G014780	Bottle gourd (USVL1VR-Ls)	lsilsiB006
Lsi05G014780	Cucumber (Gy14) v1	cgylsiB586
Lsi05G014780	Cucurbita maxima (Rimu)	cmalsiB250
Lsi05G014780	Cucurbita maxima (Rimu)	cmalsiB353
Lsi05G014780	Cucurbita maxima (Rimu)	cmalsiB757
Lsi05G014780	Cucurbita maxima (Rimu)	cmalsiB834
Lsi05G014780	Cucurbita moschata (Rifu)	cmolsiB238
Lsi05G014780	Cucurbita moschata (Rifu)	cmolsiB342
Lsi05G014780	Cucurbita moschata (Rifu)	cmolsiB752
Lsi05G014780	Cucurbita moschata (Rifu)	cmolsiB819
Lsi05G014780	Cucurbita pepo (Zucchini)	cpelsiB118
Lsi05G014780	Cucurbita pepo (Zucchini)	cpelsiB273
Lsi05G014780	Cucurbita pepo (Zucchini)	cpelsiB512
Lsi05G014780	Wild cucumber (PI 183967)	cpilsiB467
Lsi05G014780	Cucumber (Chinese Long) v2	culsiB460
Lsi05G014780	Melon (DHL92) v3.5.1	lsimeB342
Lsi05G014780	Watermelon (Charleston Gray)	lsiwcgB345
Lsi05G014780	Watermelon (97103) v1	lsiwmB384
Lsi05G014780	Cucumber (Gy14) v2	cgyblsiB428
Lsi05G014780	Melon (DHL92) v3.6.1	lsimedB359
Lsi05G014780	Silver-seed gourd	carlsiB065
Lsi05G014780	Silver-seed gourd	carlsiB298
Lsi05G014780	Cucumber (Chinese Long) v3	cuclsiB489