CsaV3_3G013940 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_3G013940
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	tRNA/rRNA methyltransferase
Location	chr3 : 10450616 .. 10452483 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATCGAAAGCTACGTTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCATGGCCCGAAGCGCCACCGCCTTTGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCTACTTCTCATCTCCGCTTCCGCCATTTTCATTCCCTTCTCGACGCTCAAACTTTCCTCAAGGTTTTGTTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTCCCTTTCGTAGTAATGTAATCATGGGTTTGTTTTGGAATTTGAATTGTGTTCTTCGGTTCTGTAGGAGAAGGATTGCGATATATGTGGTGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACTGCTTTTCTTCTTGGAAACGAGGTTGGTTGTTCGTTTTAGTTTTATTGGCTTTTCATTGATTGTGATCTCTATATTCTTGTATTGGAACTCCCTTGTTTGATCTGTCCCTATCTATTTCCAGGGAATTTCTGTACGCTGTAACTCATAATGTTGAATGAATAGAGTTGTTTTCTATATGCTGCAGTCGGTTAACAAGTTCCTAATTCAGTTGATACTTATGACTTAAGAACATTTTTATCTCCTCGAGTTGATCTTGAGGTGGGCTAAACATCCGCGAGACTAGTGGGGTTGGCCATGAGTTATATAATGTTAGGAATGAGCTTTGCTAGCATGGTAAAAAATGATAATTACTTGATAGTTTCTCTCGCAATAATGTTTTCTATAATGCTAATGCACTATTTTTCCACCTTTGAAATGATGAAATTAGGACCTATTATGGCTGGCATTGTAGATCTCTTGCTTTATTGTAGGATCTGTTGTCTGGACCGAATTGGATCAACATCATTTCGAAATTAATTTGACACTTTTCAGCTTTGAGTCACCCTTTTGATGGTTATATGCACGTTGTTTTTAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCGATCTGCGACTTCTTCGTCTACATCCCACAATATGGTGTTGGCACTGCGTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGGTAATGATCCAGACCTTTGCATTTTGATCTCCCTCTTTTGAGTTTCTTTTGCGTACATGTCTAGTGTACATCACCTGTAAGAATTATGAGTCTCTTTTGCGTACATGTCTACTTTTGGTTTTTACCAAGCAAAACTGTGAGTATTGTAAAATTATGAAATCTATGAAAGGAGGACTATAGGAAGAAACATACGTATATCTTAGAAGTTGGAACTTAGAAGAATAGTTGTTTGATCTAATAATTTTTCCCATTTCTCCTGAGTCTTGAATCAATTCTTCATGCTGGAAAATGATCCCTTCAAGCTTAAACTAAGGAAACTGTGTCCTAGGAATTACTATGGCTGAGTTGAATTGAATAATGTGGCAGTTTGTACTGGCGCACATAGTTGATTTTGATGGTGACCATCTAGGTAGATGGATCCTCTCACATGTTTCTAGTGTGCATATATTGAGATTTAAGTGAAAATCGAGTAAGTTATGTCTTATTTGATTTAATGGAAATGATACTTGTCTCTTTCTCTAGACATAACTTTAAATTTGTGTACTGTATTTTTCAGTTTGGGCAGGATTTCCTGAAAGATGTCGCGACGGTAGCAAATTTATAGTGGCTGAAAGACCAGTCAGACAGGTCAAGCGGAATTATTGCACTGAAACAGAAGAATCTATTATGGAAGAGCGTAAATCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGGATGATTGATGAATCAGCATCCAACCTTCTTGATACATTGTTTATTGATCAATAA

mRNA sequence

ATGGGGATCGAAAGCTACGTTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCATGGCCCGAAGCGCCACCGCCTTTGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCTACTTCTCATCTCCGCTTCCGCCATTTTCATTCCCTTCTCGACGCTCAAACTTTCCTCAAGGAGAAGGATTGCGATATATGTGGTGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACTGCTTTTCTTCTTGGAAACGAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCGATCTGCGACTTCTTCGTCTACATCCCACAATATGGTGTTGGCACTGCGTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCTGAAAGATGTCGCGACGGTAGCAAATTTATAGTGGCTGAAAGACCAGTCAGACAGGTCAAGCGGAATTATTGCACTGAAACAGAAGAATCTATTATGGAAGAGCGTAAATCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGGATGATTGATGAATCAGCATCCAACCTTCTTGATACATTGTTTATTGATCAATAA

Coding sequence (CDS)

ATGGGGATCGAAAGCTACGTTGTGGTGCATAACATAGCCAAAAGGCACAACGTTGGCACCATGGCCCGAAGCGCCACCGCCTTTGGCGTCTCCGAACTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCTACTTCTCATCTCCGCTTCCGCCATTTTCATTCCCTTCTCGACGCTCAAACTTTCCTCAAGGAGAAGGATTGCGATATATGTGGTGTGGAGATCACTGACAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACTGCTTTTCTTCTTGGAAACGAGGGAACAGGGCTTTCTGCTAAAGAGTGTGCGATCTGCGACTTCTTCGTCTACATCCCACAATATGGTGTTGGCACTGCGTCTTTGAATGTAACTGTAGCTGCTTCCATTGTCCTACATCATTTTGGTGTTTGGGCAGGATTTCCTGAAAGATGTCGCGACGGTAGCAAATTTATAGTGGCTGAAAGACCAGTCAGACAGGTCAAGCGGAATTATTGCACTGAAACAGAAGAATCTATTATGGAAGAGCGTAAATCCAGGAGGGAGAATGCTTCTAAAGGCTTCTTTGATGAGGGGATGATTGATGAATCAGCATCCAACCTTCTTGATACATTGTTTATTGATCAATAA

Protein sequence

MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVYIPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEESIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ

Homology

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BLAST of CsaV3_3G013940 vs. NCBI nr
Match: XP_004134172.1 (PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis sativus] >KGN57046.1 hypothetical protein Csa_3G150750 [Cucumis sativus])

HSP 1 Score: 444.1 bits (1141), Expect = 2.7e-121
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ
Sbjct: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 218

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BLAST of CsaV3_3G013940 vs. NCBI nr
Match: XP_008438758.1 (PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo])

HSP 1 Score: 435.6 bits (1119), Expect = 9.7e-119
Identity = 211/218 (96.79%), Postives = 216/218 (99.08%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPVRQVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SI+EERKSRRENASKGFFDEGMID+S SNLLDTLFIDQ
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

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BLAST of CsaV3_3G013940 vs. NCBI nr
Match: XP_022138159.1 (uncharacterized protein LOC111009398 [Momordica charantia])

HSP 1 Score: 421.4 bits (1082), Expect = 1.9e-114
Identity = 201/217 (92.63%), Postives = 213/217 (98.16%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHSL 61
           GI+SYVVVHNIAKRHN+GT+ARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHSL
Sbjct: 8   GIQSYVVVHNIAKRHNIGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHSL 67

Query: 62  LDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVYI 121
           LDAQ FLKEKDCDICGVEIT+NAVAVNQHPF++STAFLLGNEGTGLSAKECAICDFF+YI
Sbjct: 68  LDAQNFLKEKDCDICGVEITENAVAVNQHPFRKSTAFLLGNEGTGLSAKECAICDFFIYI 127

Query: 122 PQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEES 181
           PQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPV+QVKRNYC ET ES
Sbjct: 128 PQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQVKRNYCAETAES 187

Query: 182 IMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           I+EERKSRRENASKGFFDEGMID+S SNLLDTLFID+
Sbjct: 188 IIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDE 224

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BLAST of CsaV3_3G013940 vs. NCBI nr
Match: XP_022953920.1 (uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata])

HSP 1 Score: 415.6 bits (1067), Expect = 1.0e-112
Identity = 199/218 (91.28%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSE+ILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 86  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 145

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDA+ FL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 146 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 205

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPV+Q KRNYC ETEE
Sbjct: 206 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 265

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SI+EERKSRR+NASKGFFDEG ID+S SNLLDTLFI++
Sbjct: 266 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 303

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BLAST of CsaV3_3G013940 vs. NCBI nr
Match: XP_022953849.1 (uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata])

HSP 1 Score: 415.6 bits (1067), Expect = 1.0e-112
Identity = 199/218 (91.28%), Postives = 212/218 (97.25%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSE+ILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 93  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 152

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDA+ FL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 153 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 212

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPV+Q KRNYC ETEE
Sbjct: 213 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 272

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SI+EERKSRR+NASKGFFDEG ID+S SNLLDTLFI++
Sbjct: 273 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 310

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BLAST of CsaV3_3G013940 vs. TAIR10
Match: AT4G15520.1 (tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 349.4 bits (895), Expect = 1.7e-96
Identity = 171/220 (77.73%), Postives = 195/220 (88.64%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHSL 61
           G ESYVVVHNIAKRHNVGT+ARSATAFGV+ELILVGRRDFNAFGSHGS SH+RFRHFHSL
Sbjct: 3   GFESYVVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSASHIRFRHFHSL 62

Query: 62  LDAQTFLK-EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 121
           ++A+ +LK EKDCDICGVEI D A AVN+HPFKR+TAFLLGNEG+GLSAKE  ICDFFVY
Sbjct: 63  IEARNYLKEEKDCDICGVEIADGASAVNEHPFKRNTAFLLGNEGSGLSAKEYEICDFFVY 122

Query: 122 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 181
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDGSKFIVA+RPVRQ +RN+C  TEE
Sbjct: 123 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERVRDGSKFIVADRPVRQGRRNFCAGTEE 182

Query: 182 SIMEERKSRRENASKGFFDEGMID--ESASNLLDTLFIDQ 219
           S++EERK R+E+A  GFFD+   +   S+S+LLD LF+++
Sbjct: 183 SVIEERKLRKESAENGFFDDNGNENGSSSSDLLDGLFLNE 222

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BLAST of CsaV3_3G013940 vs. TAIR10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-07
Identity = 48/193 (24.87%), Postives = 81/193 (41.97%), Query Frame = 0


Query: 7   VVVHNIAKRHNVGTMARSATAFGVSELILVG-----RRDFNAFGSHGSTSHLRFRHFHSL 66
           +VV  ++   N+    RSA A G+  + +V      R + N   S G+   L    + + 
Sbjct: 157 LVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTP 216

Query: 67  LDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVYI 126
            +    LK +   I    +  + V++    +   TA ++GNEG G+S +   + D    I
Sbjct: 217 KECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSI 276

Query: 127 PQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEES 186
           P  G+   S NV+VAA I++HH                  V++R  R       +E E+ 
Sbjct: 277 PMNGM-VDSFNVSVAAGILMHH-----------------AVSDRTTRLGSHGDLSEAEKE 331

Query: 187 IMEERKSRRENAS 195
           I+    S R + S
Sbjct: 337 ILMAEFSLRHSRS 331

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BLAST of CsaV3_3G013940 vs. Swiss-Prot
Match: sp|Q4L3J1|TRMHL_STAHJ (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=SH2477 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.0e-10
Identity = 45/146 (30.82%), Postives = 71/146 (48.63%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFN-----AFGSHGSTSHLRFR 61
           G+ + V++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  H+   
Sbjct: 93  GLSTVVILDGLEDPHNLGSILRTADASGVDGVIIPKRRSVALTQTVAKASTGAIQHVPVM 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LKE    + G E  DNA            A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKEHGYWVAGAE-ADNATDYRDMAADMPLAIVIGSEGQGMSRLVKDKCD 212

Query: 122 FFVYIPQYGVGTASLNVTVAASIVLH 143
           F++ IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of CsaV3_3G013940 vs. Swiss-Prot
Match: sp|Q99W72|TRMHL_STAAM (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=SAV0531 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFN-----AFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFVYIPQYGVGTASLNVTVAASIVLH 143
           F++ IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of CsaV3_3G013940 vs. Swiss-Prot
Match: sp|Q7A794|TRMHL_STAAN (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain N315) OX=158879 GN=SA0490 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFN-----AFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFVYIPQYGVGTASLNVTVAASIVLH 143
           F++ IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of CsaV3_3G013940 vs. Swiss-Prot
Match: sp|Q6GJD7|TRMHL_STAAR (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=SAR0535 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFN-----AFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFVYIPQYGVGTASLNVTVAASIVLH 143
           F++ IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of CsaV3_3G013940 vs. Swiss-Prot
Match: sp|Q6GBV6|TRMHL_STAAS (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=SAS0489 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 43/146 (29.45%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFN-----AFGSHGSTSHLRFR 61
           G+ + +++  +   HN+G++ R+A A GV  +I+  RR        A  S G+  H+   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L      LK+    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFVYIPQYGVGTASLNVTVAASIVLH 143
           F++ IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of CsaV3_3G013940 vs. TrEMBL
Match: tr|A0A0A0LAH1|A0A0A0LAH1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150750 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.8e-121
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ
Sbjct: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 218

[Close]

BLAST of CsaV3_3G013940 vs. TrEMBL
Match: tr|A0A1S3AXA1|A0A1S3AXA1_CUCME (putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483771 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.4e-119
Identity = 211/218 (96.79%), Postives = 216/218 (99.08%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFF+Y
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDG+KFIVAERPVRQVKRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFIDQ 219
           SI+EERKSRRENASKGFFDEGMID+S SNLLDTLFIDQ
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of CsaV3_3G013940 vs. TrEMBL
Match: tr|A0A2N9IXH8|A0A2N9IXH8_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56876 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 8.4e-103
Identity = 182/217 (83.87%), Postives = 200/217 (92.17%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           M +E YVVVHNIAKRHN+GT+ARSATAFGVSE+ILVGRRDFNAFGSHGSTSHL FRHFHS
Sbjct: 1   MSVECYVVVHNIAKRHNIGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHLSFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           L +A+ FLKEKDCDICGVEITD AVAVNQHPFK+STAFLLGNEGTGLSAKEC +CDFFVY
Sbjct: 61  LSEARQFLKEKDCDICGVEITDTAVAVNQHPFKKSTAFLLGNEGTGLSAKECEVCDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYG GTASLNVTVAASIVLH+FGVWAGFPER RDGSKF+VAERPV+QV+RN+C ET +
Sbjct: 121 IPQYGCGTASLNVTVAASIVLHNFGVWAGFPERTRDGSKFVVAERPVKQVRRNFCAETAD 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFID 218
           +I+EERKSRRENAS GFFDE  ++ SASNLLD LF D
Sbjct: 181 TIIEERKSRRENASNGFFDESGVNNSASNLLDALFSD 217

[Close]

BLAST of CsaV3_3G013940 vs. TrEMBL
Match: tr|A5AVL9|A5AVL9_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_05s0020g02300 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.9e-102
Identity = 186/217 (85.71%), Postives = 198/217 (91.24%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           M +ESYVVVHNIAKRHNVGT+ARSA+A GVSELILVGRRDFN+FGSHGSTSHLRFRHFHS
Sbjct: 1   MVVESYVVVHNIAKRHNVGTLARSASALGVSELILVGRRDFNSFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           LLDA+ FLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLS KEC ICDFFVY
Sbjct: 61  LLDARHFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSVKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDG+KF+VAERP +QV+RNYCTET +
Sbjct: 121 IPQYGGGTASLNVTVAASIVLHHFGVWAGFSERIRDGNKFVVAERPAQQVRRNYCTETAD 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFID 218
           SI+EERK RR+NAS GFFDE    ES SNLLD LF D
Sbjct: 181 SIIEERKIRRQNASNGFFDESGSGESPSNLLDALFGD 217

[Close]

BLAST of CsaV3_3G013940 vs. TrEMBL
Match: tr|A0A2I4F315|A0A2I4F315_9ROSI (uncharacterized protein LOC108995036 OS=Juglans regia OX=51240 GN=LOC108995036 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.4e-102
Identity = 185/217 (85.25%), Postives = 197/217 (90.78%), Query Frame = 0


Query: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60
           M IE YVVVHNIAKRHNVGT+ARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS
Sbjct: 1   MSIECYVVVHNIAKRHNVGTLARSATAFGVSELILVGRRDFNAFGSHGSTSHLRFRHFHS 60

Query: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFVY 120
           L DA+ FLKEKDCDICGVEITD A+AVNQHPF +STAFLLGNEGTGLSAKEC ICDFFVY
Sbjct: 61  LPDARQFLKEKDCDICGVEITDTAMAVNQHPFNKSTAFLLGNEGTGLSAKECEICDFFVY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGSKFIVAERPVRQVKRNYCTETEE 180
           IPQYG GTASLNVTVAASIVLHHFGVWAGFPER RDG+K++VAERPV+Q +RNYC ET +
Sbjct: 121 IPQYGFGTASLNVTVAASIVLHHFGVWAGFPERTRDGNKYVVAERPVKQGRRNYCAETAD 180

Query: 181 SIMEERKSRRENASKGFFDEGMIDESASNLLDTLFID 218
           +I+EERKSRRENAS GFFDE     SASNLLD LF D
Sbjct: 181 TIIEERKSRRENASNGFFDESGNSNSASNLLDALFGD 217

The following BLAST results are available for this feature:

BLAST of CsaV3_3G013940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134172.1	2.7e-121	100.00	PREDICTED: rRNA methyltransferase 1, mitochondrial [Cucumis sativus] >KGN57046.1...	[more]
XP_008438758.1	9.7e-119	96.79	PREDICTED: putative TrmH family tRNA/rRNA methyltransferase [Cucumis melo]	[more]
XP_022138159.1	1.9e-114	92.63	uncharacterized protein LOC111009398 [Momordica charantia]	[more]
XP_022953920.1	1.0e-112	91.28	uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata]	[more]
XP_022953849.1	1.0e-112	91.28	uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata]	[more]

BLAST of CsaV3_3G013940 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G15520.1	1.7e-96	77.73	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT5G15390.1	1.9e-07	24.87	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

BLAST of CsaV3_3G013940 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q4L3J1\|TRMHL_STAHJ	5.0e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus ...	[more]
sp\|Q99W72\|TRMHL_STAAM	1.5e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
sp\|Q7A794\|TRMHL_STAAN	1.5e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
sp\|Q6GJD7\|TRMHL_STAAR	1.5e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
sp\|Q6GBV6\|TRMHL_STAAS	1.5e-09	29.45	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]

BLAST of CsaV3_3G013940 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LAH1\|A0A0A0LAH1_CUCSA	1.8e-121	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150750 PE=4 SV=1	[more]
tr\|A0A1S3AXA1\|A0A1S3AXA1_CUCME	6.4e-119	96.79	putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]
tr\|A0A2N9IXH8\|A0A2N9IXH8_FAGSY	8.4e-103	83.87	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56876 PE=4 SV=1	[more]
tr\|A5AVL9\|A5AVL9_VITVI	1.9e-102	85.71	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_05s0020g02300 PE=4 SV=...	[more]
tr\|A0A2I4F315\|A0A2I4F315_9ROSI	2.4e-102	85.25	uncharacterized protein LOC108995036 OS=Juglans regia OX=51240 GN=LOC108995036 P...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008173	RNA methyltransferase activity
GO:0003723	RNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006396	RNA processing

Vocabulary: **INTERPRO**
Term	Definition
IPR029028	Alpha/beta_knot_MTases
IPR001537	SpoU_MeTrfase
IPR029026	tRNA_m1G_MTases_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009451	RNA modification
biological_process	GO:0006396	RNA processing
biological_process	GO:0006635	fatty acid beta-oxidation
biological_process	GO:0016558	protein import into peroxisome matrix
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G013940.1	CsaV3_3G013940.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	G3DSA:3.40.1280.10		coord: 3..148 e-value: 1.9E-35 score: 124.0
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 6..142 e-value: 1.2E-28 score: 100.0
None	No IPR available	PANTHER	PTHR43191	FAMILY NOT NAMED	coord: 3..155
None	No IPR available	PANTHER	PTHR43191:SF1	OBP33PEP LIKE PROTEIN	coord: 3..155
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	SSF75217	alpha/beta knot	coord: 5..145

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_3G013940	CSPI03G13710	Wild cucumber (PI 183967)	cpicucB144
CsaV3_3G013940	Cucsa.253780	Cucumber (Gy14) v1	cgycucB387
CsaV3_3G013940	Cp4.1LG08g04360	Cucurbita pepo (Zucchini)	cpecucB1056
CsaV3_3G013940	CsGy3G013860	Cucumber (Gy14) v2	cgybcucB117
CsaV3_3G013940	Carg17385	Silver-seed gourd	carcucB0640
CsaV3_3G013940	Carg20156	Silver-seed gourd	carcucB0878
CsaV3_3G013940	Cla021684	Watermelon (97103) v1	cucwmB239
CsaV3_3G013940	MELO3C006586	Melon (DHL92) v3.5.1	cucmeB236
CsaV3_3G013940	ClCG05G005600	Watermelon (Charleston Gray)	cucwcgB225
CsaV3_3G013940	Lsi05G014780	Bottle gourd (USVL1VR-Ls)	cuclsiB218
CsaV3_3G013940	MELO3C006586.2	Melon (DHL92) v3.6.1	cucmedB230
CsaV3_3G013940	Cla97C05G087000	Watermelon (97103) v2	cucwmbB206
CsaV3_3G013940	Bhi01G000653	Wax gourd	cucwgoB272

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_3G013940	Wax gourd	cucwgoB261
CsaV3_3G013940	Cucumber (Chinese Long) v3	cuccucB141
CsaV3_3G013940	Silver-seed gourd	carcucB0078
CsaV3_3G013940	Silver-seed gourd	carcucB0355
CsaV3_3G013940	Cucumber (Gy14) v2	cgybcucB271
CsaV3_3G013940	Cucumber (Gy14) v1	cgycucB554
CsaV3_3G013940	Cucurbita maxima (Rimu)	cmacucB0291
CsaV3_3G013940	Cucurbita maxima (Rimu)	cmacucB0425
CsaV3_3G013940	Cucurbita maxima (Rimu)	cmacucB0965
CsaV3_3G013940	Cucurbita maxima (Rimu)	cmacucB1049
CsaV3_3G013940	Cucurbita moschata (Rifu)	cmocucB0283
CsaV3_3G013940	Cucurbita moschata (Rifu)	cmocucB0420
CsaV3_3G013940	Cucurbita moschata (Rifu)	cmocucB0953
CsaV3_3G013940	Cucurbita moschata (Rifu)	cmocucB1032
CsaV3_3G013940	Cucurbita pepo (Zucchini)	cpecucB0154
CsaV3_3G013940	Cucurbita pepo (Zucchini)	cpecucB0385
CsaV3_3G013940	Cucurbita pepo (Zucchini)	cpecucB0759
CsaV3_3G013940	Wild cucumber (PI 183967)	cpicucB339
CsaV3_3G013940	Bottle gourd (USVL1VR-Ls)	cuclsiB163
CsaV3_3G013940	Melon (DHL92) v3.5.1	cucmeB176
CsaV3_3G013940	Melon (DHL92) v3.6.1	cucmedB167
CsaV3_3G013940	Watermelon (Charleston Gray)	cucwcgB254
CsaV3_3G013940	Watermelon (97103) v1	cucwmB288
CsaV3_3G013940	Watermelon (97103) v2	cucwmbB233