Lsi04G000180 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi04G000180
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSigma factor binding protein 1
Locationchr04 : 250903 .. 251337 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTAACCGGCAAAAAACAGGCAAAAAGAAGCAAGAAATCTGCGGCGGCCGATTTCAAAGTCGTGTACATTTCAAGTCCGATGAAGGTGAAAACCAGCGCCTCGAAGTTCCGATCTCTCGTTCAGAAACTCACCGGTCAGGATTCCGATGCCGAGAGGTTCATGGAACTGATCGCCTCCGCTGCTAGTGATCGGAATCTTCGATCGTCAGCGTCGTTCCTCGACTACCAACTCCTCGGCGACGACGATCTAATGGTGGCAGGAAAGGTCGAAACGGCGGCGTATGAATCGTCGGTTGCGGCTCTGCCGCCGCCGGATGATTTGGCGGTTCTGCAGAATTTCGATGCAAGTTTTGCAGAAATGTTCCATGGCTTTTGGTATGATTCTTCTACGGCACAAACTGAGGTGCCAGGTTTAAGTGCACTGCTGTAG

mRNA sequence

ATGAATGTAACCGGCAAAAAACAGGCAAAAAGAAGCAAGAAATCTGCGGCGGCCGATTTCAAAGTCGTGTACATTTCAAGTCCGATGAAGGTGAAAACCAGCGCCTCGAAGTTCCGATCTCTCGTTCAGAAACTCACCGGTCAGGATTCCGATGCCGAGAGGTTCATGGAACTGATCGCCTCCGCTGCTAGTGATCGGAATCTTCGATCGTCAGCGTCGTTCCTCGACTACCAACTCCTCGGCGACGACGATCTAATGGTGGCAGGAAAGGTCGAAACGGCGGCGTATGAATCGTCGGTTGCGGCTCTGCCGCCGCCGGATGATTTGGCGGTTCTGCAGAATTTCGATGCAAGTTTTGCAGAAATGTTCCATGGCTTTTGGTATGATTCTTCTACGGCACAAACTGAGGTGCCAGGTTTAAGTGCACTGCTGTAG

Coding sequence (CDS)

ATGAATGTAACCGGCAAAAAACAGGCAAAAAGAAGCAAGAAATCTGCGGCGGCCGATTTCAAAGTCGTGTACATTTCAAGTCCGATGAAGGTGAAAACCAGCGCCTCGAAGTTCCGATCTCTCGTTCAGAAACTCACCGGTCAGGATTCCGATGCCGAGAGGTTCATGGAACTGATCGCCTCCGCTGCTAGTGATCGGAATCTTCGATCGTCAGCGTCGTTCCTCGACTACCAACTCCTCGGCGACGACGATCTAATGGTGGCAGGAAAGGTCGAAACGGCGGCGTATGAATCGTCGGTTGCGGCTCTGCCGCCGCCGGATGATTTGGCGGTTCTGCAGAATTTCGATGCAAGTTTTGCAGAAATGTTCCATGGCTTTTGGTATGATTCTTCTACGGCACAAACTGAGGTGCCAGGTTTAAGTGCACTGCTGTAG

Protein sequence

MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIASAASDRNLRSSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDASFAEMFHGFWYDSSTAQTEVPGLSALL
BLAST of Lsi04G000180 vs. Swiss-Prot
Match: SIB1_ARATH (Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana GN=SIB1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.9e-06
Identity = 41/126 (32.54%), Postives = 68/126 (53.97%), Query Frame = 1

Query: 6   KKQAKRSKKSAAAD--FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIASAA 65
           +K  K+ KK+ + +   KV YIS+PM+V+T ASKFR LVQ+LTGQD+   +   + + ++
Sbjct: 24  RKSPKQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDAVDLQPEPIYSPSS 83

Query: 66  SDRNLRSSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDASFAEMF 125
            D NL   A  L  ++L  +     G+ ++  YE   A     +D+ +     A F+  F
Sbjct: 84  DDHNLSPPAENLAPRVLHQEPF---GERDSDCYEPLNA-----EDMFLPDQMSAGFSGFF 141

Query: 126 HGFWYD 130
              +Y+
Sbjct: 144 SNGFYN 141

BLAST of Lsi04G000180 vs. TrEMBL
Match: D1MWZ4_CITLA (Sigma factor binding protein 1 OS=Citrullus lanatus subsp. vulgaris GN=CitSIB PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 1.3e-61
Identity = 129/146 (88.36%), Postives = 136/146 (93.15%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           MNVTGKKQAKRS+KSAAADFKVVYISSPMKVKTSASKFRSLVQ+LTGQDSDAERFMEL A
Sbjct: 1   MNVTGKKQAKRSRKSAAADFKVVYISSPMKVKTSASKFRSLVQRLTGQDSDAERFMELTA 60

Query: 61  SAASDRNLRSSAS--FLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDAS 120
           SAASDRNL+SSAS  F DYQLLGDDDL+VAGKVETAAYESSVAALPPPDDLAVL N DAS
Sbjct: 61  SAASDRNLQSSASATFFDYQLLGDDDLVVAGKVETAAYESSVAALPPPDDLAVLHNLDAS 120

Query: 121 FAEMFHGFWYDSSTAQTEVPGLSALL 145
           F  MFHGFW+DS TAQ E+PGL+ALL
Sbjct: 121 FGGMFHGFWFDSFTAQAEMPGLNALL 146

BLAST of Lsi04G000180 vs. TrEMBL
Match: A0A0A0KTR9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G623915 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.9e-43
Identity = 101/146 (69.18%), Postives = 120/146 (82.19%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           M VTGKKQAKRSKKSA A FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFME+ A
Sbjct: 1   MIVTGKKQAKRSKKSAGAGFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMEM-A 60

Query: 61  SAASDRNLR--SSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDAS 120
           S  SD N    +SASF+D+Q++G++D+MV GKV     E+ V  + P D+LA LQN D +
Sbjct: 61  SGGSDGNGEWWASASFVDHQVMGEEDVMVGGKV-----EAGVGGVSPLDELAFLQNLDGN 120

Query: 121 FAEMFHGFWYDSSTAQTEVPGLSALL 145
           +AEM +GFW+DSSTA++E+PGLSALL
Sbjct: 121 YAEMLNGFWHDSSTAESEMPGLSALL 140

BLAST of Lsi04G000180 vs. TrEMBL
Match: A0A067L8W8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24548 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.4e-13
Identity = 60/145 (41.38%), Postives = 83/145 (57.24%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           +N+  K+QAKRSKK+     KVVYISSPMKV+TSASKFR+LVQ+LTG+DSDA RFM++  
Sbjct: 6   VNMKNKRQAKRSKKA----IKVVYISSPMKVQTSASKFRALVQELTGKDSDAARFMDV-- 65

Query: 61  SAASDRNLRSSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFD---- 120
               D+NL SS   LD   +      V   + +    SS +    PD   V ++ D    
Sbjct: 66  -NGGDQNLNSSPEILDQTGVDGQGSFVPSLMNSCNDSSSTS----PDSDFVFESLDRYLP 125

Query: 121 ---ASFAEMFH-GFWYDSSTAQTEV 138
               SF  MF    +++ S+ + +V
Sbjct: 126 PMEGSFMSMFQSNLFHEYSSHELDV 139

BLAST of Lsi04G000180 vs. TrEMBL
Match: M5XE50_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013276mg PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.9e-09
Identity = 38/52 (73.08%), Postives = 44/52 (84.62%), Query Frame = 1

Query: 6  KKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFME 58
          K   K+SK+  +   KVVYISSPMKVKTSASKFR+LVQ+LTG+DSDAERFME
Sbjct: 10 KSPPKQSKRRKSKGVKVVYISSPMKVKTSASKFRALVQELTGRDSDAERFME 61

BLAST of Lsi04G000180 vs. TrEMBL
Match: W9RNW5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_023976 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.5e-09
Identity = 47/97 (48.45%), Postives = 62/97 (63.92%), Query Frame = 1

Query: 6  KKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIASAASD 65
          +KQ KRS+++     KVVYISSPMKVKTSAS+FR+LVQ+LTG++SDAERFME I      
Sbjct: 12 QKQFKRSRRNKGV--KVVYISSPMKVKTSASEFRALVQELTGRNSDAERFMEEINGVG-- 71

Query: 66 RNLRSSASFLDYQLLGDDD----LMVAGKVETAAYES 99
                   ++   L D D    +M +G VE  ++ES
Sbjct: 72 ---------INTANLDDQDQHQMMMKSGSVEGGSHES 95

BLAST of Lsi04G000180 vs. TAIR10
Match: AT3G56710.1 (AT3G56710.1 sigma factor binding protein 1)

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-07
Identity = 41/126 (32.54%), Postives = 68/126 (53.97%), Query Frame = 1

Query: 6   KKQAKRSKKSAAAD--FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIASAA 65
           +K  K+ KK+ + +   KV YIS+PM+V+T ASKFR LVQ+LTGQD+   +   + + ++
Sbjct: 24  RKSPKQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDAVDLQPEPIYSPSS 83

Query: 66  SDRNLRSSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDASFAEMF 125
            D NL   A  L  ++L  +     G+ ++  YE   A     +D+ +     A F+  F
Sbjct: 84  DDHNLSPPAENLAPRVLHQEPF---GERDSDCYEPLNA-----EDMFLPDQMSAGFSGFF 141

Query: 126 HGFWYD 130
              +Y+
Sbjct: 144 SNGFYN 141

BLAST of Lsi04G000180 vs. NCBI nr
Match: gi|270309002|dbj|BAI52954.1| (sigma factor binding protein 1 [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-61
Identity = 129/146 (88.36%), Postives = 136/146 (93.15%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           MNVTGKKQAKRS+KSAAADFKVVYISSPMKVKTSASKFRSLVQ+LTGQDSDAERFMEL A
Sbjct: 1   MNVTGKKQAKRSRKSAAADFKVVYISSPMKVKTSASKFRSLVQRLTGQDSDAERFMELTA 60

Query: 61  SAASDRNLRSSAS--FLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDAS 120
           SAASDRNL+SSAS  F DYQLLGDDDL+VAGKVETAAYESSVAALPPPDDLAVL N DAS
Sbjct: 61  SAASDRNLQSSASATFFDYQLLGDDDLVVAGKVETAAYESSVAALPPPDDLAVLHNLDAS 120

Query: 121 FAEMFHGFWYDSSTAQTEVPGLSALL 145
           F  MFHGFW+DS TAQ E+PGL+ALL
Sbjct: 121 FGGMFHGFWFDSFTAQAEMPGLNALL 146

BLAST of Lsi04G000180 vs. NCBI nr
Match: gi|659092245|ref|XP_008446972.1| (PREDICTED: sigma factor binding protein 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-45
Identity = 108/146 (73.97%), Postives = 120/146 (82.19%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           MNVTGKKQAKRSKKS AADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFME+ A
Sbjct: 1   MNVTGKKQAKRSKKSTAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMEM-A 60

Query: 61  SAASDRNLRS--SASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDAS 120
           S ASD N +S  SASFLD QL+G++D++V GK E A        + P DDL  LQN D +
Sbjct: 61  SGASDGNGQSWASASFLDQQLMGEEDVVVGGKEEAA-----FGGVNPADDLVFLQNLDGN 120

Query: 121 FAEMFHGFWYDSSTAQTEVPGLSALL 145
           FAEMF+GFW+DSSTAQTE+ GLSALL
Sbjct: 121 FAEMFNGFWHDSSTAQTEMTGLSALL 140

BLAST of Lsi04G000180 vs. NCBI nr
Match: gi|449449264|ref|XP_004142385.1| (PREDICTED: uncharacterized protein LOC101217123 [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 8.5e-43
Identity = 101/146 (69.18%), Postives = 120/146 (82.19%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           M VTGKKQAKRSKKSA A FKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFME+ A
Sbjct: 1   MIVTGKKQAKRSKKSAGAGFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMEM-A 60

Query: 61  SAASDRNLR--SSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFDAS 120
           S  SD N    +SASF+D+Q++G++D+MV GKV     E+ V  + P D+LA LQN D +
Sbjct: 61  SGGSDGNGEWWASASFVDHQVMGEEDVMVGGKV-----EAGVGGVSPLDELAFLQNLDGN 120

Query: 121 FAEMFHGFWYDSSTAQTEVPGLSALL 145
           +AEM +GFW+DSSTA++E+PGLSALL
Sbjct: 121 YAEMLNGFWHDSSTAESEMPGLSALL 140

BLAST of Lsi04G000180 vs. NCBI nr
Match: gi|802574171|ref|XP_012068696.1| (PREDICTED: uncharacterized protein LOC105631246 [Jatropha curcas])

HSP 1 Score: 82.0 bits (201), Expect = 9.2e-13
Identity = 60/145 (41.38%), Postives = 83/145 (57.24%), Query Frame = 1

Query: 1   MNVTGKKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFMELIA 60
           +N+  K+QAKRSKK+     KVVYISSPMKV+TSASKFR+LVQ+LTG+DSDA RFM++  
Sbjct: 6   VNMKNKRQAKRSKKA----IKVVYISSPMKVQTSASKFRALVQELTGKDSDAARFMDV-- 65

Query: 61  SAASDRNLRSSASFLDYQLLGDDDLMVAGKVETAAYESSVAALPPPDDLAVLQNFD---- 120
               D+NL SS   LD   +      V   + +    SS +    PD   V ++ D    
Sbjct: 66  -NGGDQNLNSSPEILDQTGVDGQGSFVPSLMNSCNDSSSTS----PDSDFVFESLDRYLP 125

Query: 121 ---ASFAEMFH-GFWYDSSTAQTEV 138
               SF  MF    +++ S+ + +V
Sbjct: 126 PMEGSFMSMFQSNLFHEYSSHELDV 139

BLAST of Lsi04G000180 vs. NCBI nr
Match: gi|645251336|ref|XP_008231630.1| (PREDICTED: uncharacterized protein LOC103330797 [Prunus mume])

HSP 1 Score: 72.4 bits (176), Expect = 7.3e-10
Identity = 39/52 (75.00%), Postives = 45/52 (86.54%), Query Frame = 1

Query: 6  KKQAKRSKKSAAADFKVVYISSPMKVKTSASKFRSLVQKLTGQDSDAERFME 58
          K   K+SK+S +   KVVYISSPMKVKTSASKFR+LVQ+LTG+DSDAERFME
Sbjct: 10 KSPPKQSKRSKSKGVKVVYISSPMKVKTSASKFRALVQELTGRDSDAERFME 61

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SIB1_ARATH2.9e-0632.54Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana GN=SIB1 PE... [more]
Match NameE-valueIdentityDescription
D1MWZ4_CITLA1.3e-6188.36Sigma factor binding protein 1 OS=Citrullus lanatus subsp. vulgaris GN=CitSIB PE... [more]
A0A0A0KTR9_CUCSA5.9e-4369.18Uncharacterized protein OS=Cucumis sativus GN=Csa_5G623915 PE=4 SV=1[more]
A0A067L8W8_JATCU6.4e-1341.38Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24548 PE=4 SV=1[more]
M5XE50_PRUPE1.9e-0973.08Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013276mg PE=4 SV=1[more]
W9RNW5_9ROSA9.5e-0948.45Uncharacterized protein OS=Morus notabilis GN=L484_023976 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G56710.11.6e-0732.54 sigma factor binding protein 1[more]
Match NameE-valueIdentityDescription
gi|270309002|dbj|BAI52954.1|1.8e-6188.36sigma factor binding protein 1 [Citrullus lanatus subsp. vulgaris][more]
gi|659092245|ref|XP_008446972.1|1.4e-4573.97PREDICTED: sigma factor binding protein 1, chloroplastic-like [Cucumis melo][more]
gi|449449264|ref|XP_004142385.1|8.5e-4369.18PREDICTED: uncharacterized protein LOC101217123 [Cucumis sativus][more]
gi|802574171|ref|XP_012068696.1|9.2e-1341.38PREDICTED: uncharacterized protein LOC105631246 [Jatropha curcas][more]
gi|645251336|ref|XP_008231630.1|7.3e-1075.00PREDICTED: uncharacterized protein LOC103330797 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008889VQ
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G000180.1Lsi04G000180.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008889VQPFAMPF05678VQcoord: 25..51
score: 1.0
NoneNo IPR availablePANTHERPTHR33624FAMILY NOT NAMEDcoord: 6..73
score: 1.9
NoneNo IPR availablePANTHERPTHR33624:SF5SUBFAMILY NOT NAMEDcoord: 6..73
score: 1.9