Csa1G025020 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa1G025020
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCalmodulin 24-like protein; contains IPR011992 (EF-hand-like domain)
LocationChr1 : 2661928 .. 2662918 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTACAAAAATCGAAGTTTAACCCATTCCGTTTTCCTCTCTATCAAGTTTTAACCTCCAAAAATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGATGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAGCCCTAACTCTAGGTTTTGGTAAGTTGTTTAGTAATTAGCCGCGTGAGAGAGAGGTAACGAAGTCGATTTCTATCCTTCGTTCCTTTTCCATTTCGGTTTCTTTTTTCTTCTTTTTAAATTTTCTCTGTGTTCTATCCTTAATCTGAATCCAATCCAACTGTTTTTGTTTTCTTATTTTGTCGAAATGTAACAAAAAAAAAAATTCGGATGTGCTTCTGTTGATCATCCGCCATTAATGCAGATCTTTAATTTAAACTCTTTCTATCCGATTGAAGATTGAAGAGAAACTTTTCCGGAAAATCGATTGGTATTTCCGGATTCATAGTGTGCATTGCTGTTGAGTGCTGAGTACATTTCATCCC

mRNA sequence

ATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGATGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAG

Coding sequence (CDS)

ATGGCCACCGACCCCAACCCGATTCCCACCTCAAAATCCGAAGCCGACCAAATCGCCAAGCAGAACCCATCCGTCTTACTCCAGGACGACGAAGAACTCCGCAAGGTTTTCGAACGCTTCGATGCGAATGGCGACGGTAAGATCTCCATCTCCGAGCTCGATGCCGTTCTCACTTCCTTAACGTTAAAATCCGCCATCCCCTTAGAAGAGCTTCGATCTGTCATGGATGATCTCGACTCTGATAAGGACGGGTATATCAACATCGATGAATTCGCCGCCTTCTGTAAGAAACCGATGGCCTCCGACGAAGCTGGAGCAGCGGAGCTTCGTGACGCTTTCGACCTCTACGATCAGGACCGTAACGGCCTTATTTCTCAGTCCGAGCTCCATCTCGTTCTCAATCGCCTCGGAATCAGTTGTTCTAAGGAAGACTGTCAAAAAATGATCAACTCCGTTGATTCGGATGGAGACGGAAATGTGAATTTCGAAGAGTTTAGGAAGATGATGACGGATAATTCCAAATCGAAGGCCGCTCAACAGAACGGAACTGCCGCGGCGGCACCCTAG

Protein sequence

MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAAAAP*
Homology
[Close]
BLAST of Csa1G025020 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.2e-44
Identity = 93/170 (54.71%), Postives = 123/170 (72.35%), Query Frame = 1 

Query: 17  QIAKQNPSVL-LQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + EEL+KVF++FD+NGDGKIS+ EL  V  ++   ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMG--TSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN+DEF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCRS-----SSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]
BLAST of Csa1G025020 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-41
Identity = 87/160 (54.37%), Postives = 110/160 (68.75%), Query Frame = 1 

Query: 22  NPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSD 81
           N S     D EL+KVF++FDANGDGKIS+SEL  V  S+   ++   EEL  V+D++D D
Sbjct: 10  NKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMG--TSYTEEELNRVLDEIDID 69

Query: 82  KDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCS 141
            DG+IN +EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS
Sbjct: 70  CDGFINQEEFATICRS-----SSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCS 129

Query: 142 KEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQN 182
            EDC +MI  VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 130 VEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of Csa1G025020 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.8e-38
Identity = 86/154 (55.84%), Postives = 108/154 (70.13%), Query Frame = 1 

Query: 23  PSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDK 82
           PSV LQ+  E++ VF RFDANGDGKIS  EL  VL +L   ++   EE+  +M+++D+DK
Sbjct: 11  PSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTS--KEEIGRIMEEIDTDK 70

Query: 83  DGYINIDEFAAFCKK---PMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGIS 142
           DG+IN+ EFAAF K    P  S   G  EL++AF+LYDQD NGLIS  ELH +L RLG  
Sbjct: 71  DGFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGER 130

Query: 143 CSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNS 174
            ++ DC +MI SVDSDGDG V+FEEF+KMMT+ S
Sbjct: 131 YAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKS 161

[Close]
BLAST of Csa1G025020 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 7.2e-34
Identity = 74/146 (50.68%), Postives = 102/146 (69.86%), Query Frame = 1 

Query: 31  EELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDE 90
           E+++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I++DE
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]
BLAST of Csa1G025020 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.0e-33
Identity = 76/149 (51.01%), Postives = 98/149 (65.77%), Query Frame = 1 

Query: 32  ELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEF 91
           EL +VF R+DANGDGKIS  EL +VL +L   + +   E+R +MD++DSD+DG++++ EF
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALG--APLGPGEVRRMMDEMDSDRDGFVDLSEF 64

Query: 92  AAFCKKPMAS-----------DEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISC 151
           AAF   P  +           + A  AELR+AF +YD D NG IS  ELH VL +LG  C
Sbjct: 65  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 124

Query: 152 SKEDCQKMINSVDSDGDGNVNFEEFRKMM 170
           S  DC +MI SVD+DGDG VNF+EF+KMM
Sbjct: 125 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of Csa1G025020 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 6.9e-100
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of Csa1G025020 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.9e-50
Identity = 103/173 (59.54%), Postives = 133/173 (76.88%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MAT+P     + +E +   K  PSV ++D +EL++VF RFDANGDGKIS++ELD VL +L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S +P +EL  VM DLD+D DG+IN+ EFAAFC+   A  + GA+ELR+AFDLYDQD+
Sbjct: 61  G--STVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDK 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNS 174
           NGLIS +EL LVLNRLG+ CS E+C  MI SVDSDGDGNVNF+EF++MMT+N+
Sbjct: 121 NGLISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of Csa1G025020 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 6.1e-48
Identity = 104/183 (56.83%), Postives = 131/183 (71.58%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MAT+P       +  +  A    SV LQD EEL++VF RFDANGDGKIS+SELD VL SL
Sbjct: 1   MATNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S +P EEL+ VM+DLD+D DG+IN+ EFAAFC+   A  + G  EL DAF+LYDQD+
Sbjct: 61  G--SGVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDK 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLIS +EL  VLNRLG+ CS E+C  MI SVDSDGDGNVNF EF++MM++N ++ +  +
Sbjct: 121 NGLISATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSNNRENASNAE 179

Query: 181 NGT 184
             T
Sbjct: 181 EKT 179

[Close]
BLAST of Csa1G025020 vs. TrEMBL
Match: A0A0L9ULE2_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan05g099100 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.6e-46
Identity = 99/172 (57.56%), Postives = 130/172 (75.58%), Query Frame = 1 

Query: 6   NPIPTSKSEA--DQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLK 65
           NPI T   +A  +  A    SV LQD +E+++VF RFD NGDGKIS+SELD VL SL   
Sbjct: 4   NPIATGNVDAAPNSDASNKSSVYLQDMDEMKRVFNRFDTNGDGKISVSELDNVLRSLG-- 63

Query: 66  SAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGL 125
           S +P EEL+ V+DDLD+D DG+IN+ EFAAF +   A  + G  E++DAF++YDQD+NGL
Sbjct: 64  SGVPPEELQGVIDDLDTDHDGFINLSEFAAFYRADTA--DGGDTEVQDAFNMYDQDKNGL 123

Query: 126 ISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKS 176
           IS +EL+ VLNRLG+SC+KE+C  MI SVDSDGDGNVNF EF++MM++N ++
Sbjct: 124 ISATELYQVLNRLGMSCTKEECHNMIKSVDSDGDGNVNFAEFKRMMSNNREN 171

[Close]
BLAST of Csa1G025020 vs. TrEMBL
Match: A0A0R0K5I7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 3.4e-46
Identity = 101/183 (55.19%), Postives = 130/183 (71.04%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MAT+P       +  +  A   PSV LQD EEL++VF RFDAN DGKIS++ELD VL SL
Sbjct: 37  MATNPIEAGNGDAAPNPNATTKPSVYLQDTEELKRVFSRFDANCDGKISVTELDNVLRSL 96

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S +P E+++ VMDDLD+D DG+IN+ EFAAFC+   A  + G AEL DAF+LYD D+
Sbjct: 97  G--SGVPPEDIQRVMDDLDTDHDGFINLSEFAAFCRSDTA--DGGDAELHDAFNLYDHDK 156

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NG IS +EL  VLNRLG+ CS E+C  MI SVDSDGDGNVNF EF++MM++N ++ +  +
Sbjct: 157 NGHISATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSNNRENASNGE 215

Query: 181 NGT 184
             T
Sbjct: 217 EKT 215

[Close]
BLAST of Csa1G025020 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 181.0 bits (458), Expect = 6.7e-46
Identity = 93/170 (54.71%), Postives = 123/170 (72.35%), Query Frame = 1 

Query: 17  QIAKQNPSVL-LQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVM 76
           + AK  P+ + + + EEL+KVF++FD+NGDGKIS+ EL  V  ++   ++    EL  V+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMG--TSYTETELNRVL 66

Query: 77  DDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNR 136
           +++D+D+DGYIN+DEF+  C+       + AAE+RDAFDLYDQD+NGLIS SELH VLNR
Sbjct: 67  EEVDTDRDGYINLDEFSTLCRS-----SSSAAEIRDAFDLYDQDKNGLISASELHQVLNR 126

Query: 137 LGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQNGTAA 186
           LG+SCS EDC +MI  VD+DGDGNVNFEEF+KMMT +S       NG+AA
Sbjct: 127 LGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]
BLAST of Csa1G025020 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 169.5 bits (428), Expect = 2.0e-42
Identity = 87/160 (54.37%), Postives = 110/160 (68.75%), Query Frame = 1 

Query: 22  NPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSD 81
           N S     D EL+KVF++FDANGDGKIS+SEL  V  S+   ++   EEL  V+D++D D
Sbjct: 10  NKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMG--TSYTEEELNRVLDEIDID 69

Query: 82  KDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCS 141
            DG+IN +EFA  C+       + A E+R+AFDLYDQ++NGLIS SE+H VLNRLG++CS
Sbjct: 70  CDGFINQEEFATICRS-----SSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCS 129

Query: 142 KEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQN 182
            EDC +MI  VD+DGDGNVNFEEF+KMM+     K    N
Sbjct: 130 VEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of Csa1G025020 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 145.2 bits (365), Expect = 4.1e-35
Identity = 74/146 (50.68%), Postives = 102/146 (69.86%), Query Frame = 1 

Query: 31  EELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDE 90
           E+++KVF+RFD N DGKISI EL  V+ +L+  ++   EE +++M + D D +G+I++DE
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNAS--QEETKAMMKEFDLDGNGFIDLDE 73

Query: 91  FAA-FCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMI 150
           F A F     +S+ +   +L++AFDLYD DRNG IS +ELH V+  LG  CS +DCQ+MI
Sbjct: 74  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 133

Query: 151 NSVDSDGDGNVNFEEFRKMMTDNSKS 176
           N VDSDGDG V+FEEF+KMM  N  +
Sbjct: 134 NKVDSDGDGCVDFEEFKKMMMINGSA 157

[Close]
BLAST of Csa1G025020 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 133.3 bits (334), Expect = 1.6e-31
Identity = 71/143 (49.65%), Postives = 89/143 (62.24%), Query Frame = 1 

Query: 32  ELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDEF 91
           EL  VF++FD NGDGKIS  EL A++TSL  +  +P EEL   + ++D   DGYIN +EF
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHE--VPEEELEKAITEIDRKGDGYINFEEF 96

Query: 92  AAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKMINS 151
                K M  ++     L+DAF +YD D NG IS  ELH VL  LG  CS  +C+KMI  
Sbjct: 97  VELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGG 156

Query: 152 VDSDGDGNVNFEEFRKMMTDNSK 175
           VD DGDG ++FEEF+ MMT  S+
Sbjct: 157 VDKDGDGTIDFEEFKIMMTMGSR 176

[Close]
BLAST of Csa1G025020 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 131.7 bits (330), Expect = 4.7e-31
Identity = 68/144 (47.22%), Postives = 99/144 (68.75%), Query Frame = 1 

Query: 31  EELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINIDE 90
           ++++KVF+RFD NGDGKIS+ EL  V+ +L+  +A P EE  ++M   D D +G+I++DE
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALS-PTASP-EETVTMMKQFDLDGNGFIDLDE 75

Query: 91  FAAFCKKPMA---SDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQK 150
           F A  +  +    ++    ++L++AF+LYD D NG IS  ELH V+  LG  CS +DC+K
Sbjct: 76  FVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKK 135

Query: 151 MINSVDSDGDGNVNFEEFRKMMTD 172
           MI+ VD DGDG VNF+EF+KMM++
Sbjct: 136 MISKVDIDGDGCVNFDEFKKMMSN 157

[Close]
BLAST of Csa1G025020 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 9.9e-100
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of Csa1G025020 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 356.7 bits (914), Expect = 2.5e-95
Identity = 179/188 (95.21%), Postives = 186/188 (98.94%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MATDPN IPTS+S+ADQIAKQ PSVLL+DD+ELRKVFERFDANGDGKISI+ELDAVLTSL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
           TLKSAIPLEELRSVMDDLDSDKDGYINI+EFAAFCKKPMASDEAGAAELRDAFDLYDQDR
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA+Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAAP 189
           NGTAAAAP
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of Csa1G025020 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 206.5 bits (524), Expect = 4.2e-50
Identity = 103/173 (59.54%), Postives = 133/173 (76.88%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MAT+P     + +E +   K  PSV ++D +EL++VF RFDANGDGKIS++ELD VL +L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S +P +EL  VM DLD+D DG+IN+ EFAAFC+   A  + GA+ELR+AFDLYDQD+
Sbjct: 61  G--STVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDK 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNS 174
           NGLIS +EL LVLNRLG+ CS E+C  MI SVDSDGDGNVNF+EF++MMT+N+
Sbjct: 121 NGLISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of Csa1G025020 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 202.6 bits (514), Expect = 6.1e-49
Identity = 103/166 (62.05%), Postives = 131/166 (78.92%), Query Frame = 1 

Query: 10  TSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLE 69
           TS SE++   K   SV +Q+ +E++KVF +FDANGDGKISI+EL+ VL   TL S +P E
Sbjct: 4   TSPSESE---KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLE--TLGSEVPQE 63

Query: 70  ELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSEL 129
           EL  VMDDLD+D+DG+I +DEFAAFC+    +++A  +ELRDAFDLYD+D NGLIS  EL
Sbjct: 64  ELHRVMDDLDTDRDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEEL 123

Query: 130 HLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKS 176
           HLVLNRLG++CS EDC++MI SVDSDGDGNVNF EF+KMMT+   +
Sbjct: 124 HLVLNRLGMNCSPEDCRRMIRSVDSDGDGNVNFAEFQKMMTNKGSN 162

[Close]
BLAST of Csa1G025020 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 198.7 bits (504), Expect = 8.8e-48
Identity = 104/183 (56.83%), Postives = 131/183 (71.58%), Query Frame = 1 

Query: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60
           MAT+P       +  +  A    SV LQD EEL++VF RFDANGDGKIS+SELD VL SL
Sbjct: 1   MATNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSL 60

Query: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120
              S +P EEL+ VM+DLD+D DG+IN+ EFAAFC+   A  + G  EL DAF+LYDQD+
Sbjct: 61  G--SGVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDK 120

Query: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180
           NGLIS +EL  VLNRLG+ CS E+C  MI SVDSDGDGNVNF EF++MM++N ++ +  +
Sbjct: 121 NGLISATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSNNRENASNAE 179

Query: 181 NGT 184
             T
Sbjct: 181 EKT 179

The following BLAST results are available for this feature:
BLAST of Csa1G025020 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH1.2e-4454.71Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
CML26_ARATH3.6e-4154.38Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
ALL8_OLEEU2.8e-3855.84Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML23_ARATH7.2e-3450.68Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ8.0e-3351.01Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of Csa1G025020 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA6.9e-100100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO2.9e-5059.54Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN6.1e-4856.83Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
A0A0L9ULE2_PHAAN2.6e-4657.56Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan05g099100 PE=4 SV=1[more]
A0A0R0K5I7_SOYBN3.4e-4655.19Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1[more]
BLAST of Csa1G025020 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.16.7e-4654.71 Calcium-binding EF-hand family protein[more]
AT1G73630.12.0e-4254.38 EF hand calcium-binding protein family[more]
AT1G66400.14.1e-3550.68 calmodulin like 23[more]
AT1G24620.11.6e-3149.65 EF hand calcium-binding protein family[more]
AT5G37770.14.7e-3147.22 EF hand calcium-binding protein family[more]
BLAST of Csa1G025020 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|9.9e-100100.00PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|2.5e-9595.21PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|14589311|emb|CAC43238.1|4.2e-5059.54calcium binding protein [Sesbania rostrata][more]
gi|1009165393|ref|XP_015901010.1|6.1e-4962.05PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|356524678|ref|XP_003530955.1|8.8e-4856.83PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0044464 cell part
molecular_function GO:0005509 calcium ion binding
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU096516cucumber EST collection version 3.0transcribed_cluster
CU162870cucumber EST collection version 3.0transcribed_cluster
CU173286cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G025020.1Csa1G025020.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU096516CU096516transcribed_cluster
CU162870CU162870transcribed_cluster
CU173286CU173286transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 108..169
score: 4.2E-14coord: 31..96
score: 8.4
IPR002048EF-hand domainSMARTSM00054efh_1coord: 70..98
score: 1.4E-4coord: 144..172
score: 4.5E-5coord: 32..60
score: 5.8E-6coord: 108..136
score: 2.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 28..63
score: 15.462coord: 66..101
score: 12.393coord: 104..139
score: 13.091coord: 140..175
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 28..183
score: 1.5
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 31..175
score: 1.64
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 117..129
score: -coord: 153..165
score: -coord: 41..53
score: -coord: 79..91
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 31..174
score: 7.4
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 31..174
score: 7.4

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa1G025020Cla018929Watermelon (97103) v1cuwmB095
Csa1G025020MELO3C017353Melon (DHL92) v3.5.1cumeB055
Csa1G025020ClCG06G014370Watermelon (Charleston Gray)cuwcgB082
Csa1G025020Lsi06G012900Bottle gourd (USVL1VR-Ls)culsiB071
Csa1G025020MELO3C017353.2Melon (DHL92) v3.6.1cumedB047
Csa1G025020Cla97C06G123830Watermelon (97103) v2cuwmbB079
Csa1G025020Bhi02G000096Wax gourdcuwgoB077
Csa1G025020CSPI01G04290Wild cucumber (PI 183967)cpicuB000
Csa1G025020Cucsa.111510Cucumber (Gy14) v1cgycuB137
Csa1G025020CmaCh11G003070Cucurbita maxima (Rimu)cmacuB087
Csa1G025020CmaCh10G003260Cucurbita maxima (Rimu)cmacuB050
Csa1G025020CmoCh10G003510Cucurbita moschata (Rifu)cmocuB039
Csa1G025020CmoCh11G003140Cucurbita moschata (Rifu)cmocuB076
Csa1G025020Cp4.1LG04g13590Cucurbita pepo (Zucchini)cpecuB654
Csa1G025020Cp4.1LG18g06750Cucurbita pepo (Zucchini)cpecuB363
Csa1G025020CsGy1G004180Cucumber (Gy14) v2cgybcuB001
Csa1G025020Carg09105Silver-seed gourdcarcuB0622
Csa1G025020Carg15175Silver-seed gourdcarcuB0801
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa1G025020Silver-seed gourdcarcuB0416
Csa1G025020Watermelon (97103) v2cuwmbB055
Csa1G025020Wax gourdcuwgoB040
Csa1G025020Cucumber (Chinese Long) v2cucuB000
Csa1G025020Cucumber (Gy14) v1cgycuB160
Csa1G025020Cucumber (Gy14) v1cgycuB405
Csa1G025020Cucurbita maxima (Rimu)cmacuB221
Csa1G025020Cucurbita maxima (Rimu)cmacuB393
Csa1G025020Cucurbita moschata (Rifu)cmocuB207
Csa1G025020Melon (DHL92) v3.5.1cumeB033
Csa1G025020Watermelon (Charleston Gray)cuwcgB058
Csa1G025020Watermelon (97103) v1cuwmB063
Csa1G025020Cucurbita pepo (Zucchini)cpecuB031
Csa1G025020Cucurbita pepo (Zucchini)cpecuB509
Csa1G025020Bottle gourd (USVL1VR-Ls)culsiB025
Csa1G025020Cucumber (Gy14) v2cgybcuB196
Csa1G025020Melon (DHL92) v3.6.1cumedB028