Cp4.1LG18g06750 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG18g06750
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein
LocationCp4.1LG18 : 6813941 .. 6814477 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGCAACTAACAAATCCAAGGCGGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGCAACTAACAAATCCAAGGCGGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGCAACTAACAAATCCAAGGCGGCGGGGCCCTAG

Protein sequence

MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAAGP
Homology
[Close]
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.3e-44
Identity = 90/166 (54.22%), Postives = 124/166 (74.70%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSAT---NKSKAAGP 179
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+++   N + +A P
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSNGSAAP 167

[Close]
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.0e-42
Identity = 94/155 (60.65%), Postives = 114/155 (73.55%), Query Frame = 1 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKD 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS    EE+  IME++D DKD
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  GFINIDEFAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
           GFIN+ EFAAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKS 174
           +  DC  MI +VDSDGDG V+FEEF+KMM  TNKS
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMM--TNKS 161

[Close]
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.8e-41
Identity = 80/138 (57.97%), Postives = 108/138 (78.26%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSA 170
           +DGDGNVNFEEF+KMMS+
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-33
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 9.8e-33
Identity = 73/147 (49.66%), Postives = 101/147 (68.71%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +   E+R +M+++D D+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.0e-68
Identity = 136/178 (76.40%), Postives = 156/178 (87.64%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAA 177
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+  +KSKAA
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA 178

[Close]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.1e-46
Identity = 96/169 (56.80%), Postives = 127/169 (75.15%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM+  N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.4e-46
Identity = 101/174 (58.05%), Postives = 131/174 (75.29%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAA 177
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS  N+  A+
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMS-NNRENAS 173

[Close]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match: A0A061FWP8_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.0e-44
Identity = 95/152 (62.50%), Postives = 120/152 (78.95%), Query Frame = 1 

Query: 20  KPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDY 79
           +P  S +  EN +EL+KVF++FD+N DG+IS++EL  VLK++GS+   EEL  +MED+D 
Sbjct: 9   QPNTSSVYLENMDELQKVFNQFDANKDGKISVSELGDVLKAMGSSYSKEELERVMEDIDT 68

Query: 80  DKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 139
           DKDGFIN+ EF+AFC+    +  A  SELRDAFDLYDQD NGLIS +ELHLVL RLG+  
Sbjct: 69  DKDGFINLSEFSAFCRSSSDAVSAA-SELRDAFDLYDQDKNGLISANELHLVLNRLGLKC 128

Query: 140 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSAT 171
           S++DC RMI +VDSDGDGNVNFEEF KMMSA+
Sbjct: 129 SVDDCVRMITSVDSDGDGNVNFEEFEKMMSAS 159

[Close]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match: V7CQX7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.0e-44
Identity = 95/165 (57.58%), Postives = 127/165 (76.97%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L + +E+++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIATGNGDAAPNPDAGAKSSVYLQDMDEMKRVFSRFDTNGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +M+DLD D DGFIN+ EFAAFC+   A  + G +EL+DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQNVMDDLDTDHDGFINLSEFAAFCRAETA--DGGDTELQDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMM 168
           S +EL  VL RLG+  SI++C  MI +VDSDGDG+VNFEEF++MM
Sbjct: 123 SATELCQVLNRLGMRCSIDECHSMIKSVDSDGDGDVNFEEFKRMM 165

[Close]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 179.1 bits (453), Expect = 2.4e-45
Identity = 90/166 (54.22%), Postives = 124/166 (74.70%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSAT---NKSKAAGP 179
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+++   N + +A P
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSNGSAAP 167

[Close]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 167.9 bits (424), Expect = 5.5e-42
Identity = 80/138 (57.97%), Postives = 108/138 (78.26%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSA 170
           +DGDGNVNFEEF+KMMS+
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 144.4 bits (363), Expect = 6.6e-35
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.0e-31
Identity = 66/141 (46.81%), Postives = 96/141 (68.09%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           ++++KVF RFD NGDG+IS+ EL  V+++L      EE   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 91  AFCKEPMA---SDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMI 150
           A  +  +    ++   +S+L++AF+LYD D NG IS  ELH V+  LG   S++DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 151 HTVDSDGDGNVNFEEFRKMMS 169
             VD DGDG VNF+EF+KMMS
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMS 156

[Close]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 125.9 bits (315), Expect = 2.4e-29
Identity = 66/140 (47.14%), Postives = 89/140 (63.57%), Query Frame = 1 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDE 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P EEL   + ++D   DG+IN +E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
           F     + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 4.2e-68
Identity = 136/178 (76.40%), Postives = 156/178 (87.64%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAA 177
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+  +KSKAA
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA 178

[Close]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 265.4 bits (677), Expect = 7.2e-68
Identity = 136/178 (76.40%), Postives = 156/178 (87.64%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD N I +S SDADQIAK KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL SL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INI+EFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAA 177
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+  +KSKAA
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAA 178

[Close]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 201.8 bits (512), Expect = 9.8e-49
Identity = 103/164 (62.80%), Postives = 132/164 (80.49%), Query Frame = 1 

Query: 9   TSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEE 68
           T+S S+++   KPK S  +   +E++KVF++FD+NGDG+ISITEL+ VL++LGS +P EE
Sbjct: 3   TTSPSESE---KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLETLGSEVPQEE 62

Query: 69  LRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELH 128
           L  +M+DLD D+DGFI +DEFAAFC+    +++A  SELRDAFDLYD+DHNGLIS  ELH
Sbjct: 63  LHRVMDDLDTDRDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEELH 122

Query: 129 LVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNK 173
           LVL RLG+N S EDC+RMI +VDSDGDGNVNF EF+KMM  TNK
Sbjct: 123 LVLNRLGMNCSPEDCRRMIRSVDSDGDGNVNFAEFQKMM--TNK 159

[Close]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-46
Identity = 96/169 (56.80%), Postives = 127/169 (75.15%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM+  N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 193.4 bits (490), Expect = 3.5e-46
Identity = 101/174 (58.05%), Postives = 131/174 (75.29%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKAA 177
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS  N+  A+
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMS-NNRENAS 173

The following BLAST results are available for this feature:
BLAST of Cp4.1LG18g06750 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH4.3e-4454.22Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU4.0e-4260.65Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH9.8e-4157.97Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
CML23_ARATH1.2e-3352.17Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ9.8e-3349.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of Cp4.1LG18g06750 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA3.0e-6876.40Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO1.1e-4656.80Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN2.4e-4658.05Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
A0A061FWP8_THECC1.0e-4462.50Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=... [more]
V7CQX7_PHAVU1.0e-4457.58Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1[more]
BLAST of Cp4.1LG18g06750 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.12.4e-4554.22 Calcium-binding EF-hand family protein[more]
AT1G73630.15.5e-4257.97 EF hand calcium-binding protein family[more]
AT1G66400.16.6e-3552.17 calmodulin like 23[more]
AT5G37770.12.0e-3146.81 EF hand calcium-binding protein family[more]
AT1G24620.12.4e-2947.14 EF hand calcium-binding protein family[more]
BLAST of Cp4.1LG18g06750 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|4.2e-6876.40PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|7.2e-6876.40PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|1009165393|ref|XP_015901010.1|9.8e-4962.80PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|14589311|emb|CAC43238.1|1.6e-4656.80calcium binding protein [Sesbania rostrata][more]
gi|356524678|ref|XP_003530955.1|3.5e-4658.05PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g06750.1Cp4.1LG18g06750.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 31..94
score: 2.6E-13coord: 105..167
score: 3.3
IPR002048EF-hand domainSMARTSM00054efh_1coord: 32..60
score: 1.1E-4coord: 68..96
score: 8.3E-4coord: 142..170
score: 4.1E-6coord: 106..134
score: 9.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 64..99
score: 10.831coord: 102..137
score: 13.509coord: 28..63
score: 15.071coord: 138..173
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..169
score: 2.4
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 29..168
score: 1.37
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
score: -coord: 41..53
score: -coord: 151..163
score: -coord: 115..127
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 27..172
score: 2.7
NoneNo IPR availablePANTHERPTHR10891:SF573CALCIUM-BINDING PROTEIN CML24-RELATEDcoord: 27..172
score: 2.7

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG18g06750Cucsa.111510Cucumber (Gy14) v1cgycpeB0253
Cp4.1LG18g06750CmaCh10G003260Cucurbita maxima (Rimu)cmacpeB080
Cp4.1LG18g06750CmaCh11G003070Cucurbita maxima (Rimu)cmacpeB132
Cp4.1LG18g06750CmoCh11G003140Cucurbita moschata (Rifu)cmocpeB108
Cp4.1LG18g06750CmoCh10G003510Cucurbita moschata (Rifu)cmocpeB055
Cp4.1LG18g06750Cla018929Watermelon (97103) v1cpewmB372
Cp4.1LG18g06750Csa1G025020Cucumber (Chinese Long) v2cpecuB363
Cp4.1LG18g06750MELO3C017353Melon (DHL92) v3.5.1cpemeB330
Cp4.1LG18g06750ClCG06G014370Watermelon (Charleston Gray)cpewcgB335
Cp4.1LG18g06750CSPI01G04290Wild cucumber (PI 183967)cpecpiB363
Cp4.1LG18g06750Lsi06G012900Bottle gourd (USVL1VR-Ls)cpelsiB301
Cp4.1LG18g06750MELO3C017353.2Melon (DHL92) v3.6.1cpemedB388
Cp4.1LG18g06750CsaV3_1G004290Cucumber (Chinese Long) v3cpecucB0444
Cp4.1LG18g06750Bhi02G000096Wax gourdcpewgoB0467
Cp4.1LG18g06750CsGy1G004180Cucumber (Gy14) v2cgybcpeB055
Cp4.1LG18g06750Carg15175Silver-seed gourdcarcpeB1103
Cp4.1LG18g06750Carg09105Silver-seed gourdcarcpeB0843
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG18g06750Cp4.1LG04g13590Cucurbita pepo (Zucchini)cpecpeB360
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG18g06750Cucurbita pepo (Zucchini)cpecpeB027
Cp4.1LG18g06750Cucurbita pepo (Zucchini)cpecpeB352
Cp4.1LG18g06750Cucumber (Gy14) v1cgycpeB0307
Cp4.1LG18g06750Cucurbita maxima (Rimu)cmacpeB228
Cp4.1LG18g06750Cucurbita moschata (Rifu)cmocpeB195
Cp4.1LG18g06750Cucurbita moschata (Rifu)cmocpeB391
Cp4.1LG18g06750Wild cucumber (PI 183967)cpecpiB369
Cp4.1LG18g06750Cucumber (Chinese Long) v2cpecuB369
Cp4.1LG18g06750Bottle gourd (USVL1VR-Ls)cpelsiB294
Cp4.1LG18g06750Watermelon (Charleston Gray)cpewcgB330
Cp4.1LG18g06750Watermelon (97103) v1cpewmB366
Cp4.1LG18g06750Melon (DHL92) v3.5.1cpemeB321
Cp4.1LG18g06750Cucumber (Gy14) v2cgybcpeB623
Cp4.1LG18g06750Melon (DHL92) v3.6.1cpemedB380
Cp4.1LG18g06750Cucumber (Chinese Long) v3cpecucB0449