CmoCh10G003510 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh10G003510
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionCalcium-binding allergen Ole e 8
LocationCmo_Chr10 : 1594362 .. 1594922 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG
Homology
[Close]
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.9e-45
Identity = 94/168 (55.95%), Postives = 124/168 (73.81%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  V+E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.4e-42
Identity = 93/155 (60.00%), Postives = 114/155 (73.55%), Query Frame = 1 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKD 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS    EE+  +ME++D DKD
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  GFINIDEFAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
           GFIN+ EFAAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKS 174
           +  DC  MI +VDSDGDG V+FEEF+KMM  TNKS
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMM--TNKS 161

[Close]
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-40
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  V++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-33
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-32
Identity = 73/147 (49.66%), Postives = 101/147 (68.71%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +   E+R +M+++D D+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of CmoCh10G003510 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 9.3e-73
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.7e-47
Identity = 97/169 (57.40%), Postives = 128/169 (75.74%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  VM+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmoCh10G003510 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.1e-46
Identity = 103/175 (58.86%), Postives = 132/175 (75.43%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VMEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

[Close]
BLAST of CmoCh10G003510 vs. TrEMBL
Match: V7CQX7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-44
Identity = 96/165 (58.18%), Postives = 127/165 (76.97%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L + +E+++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIATGNGDAAPNPDAGAKSSVYLQDMDEMKRVFSRFDTNGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VM+DLD D DGFIN+ EFAAFC+   A  + G +EL+DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQNVMDDLDTDHDGFINLSEFAAFCRAETA--DGGDTELQDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMM 168
           S +EL  VL RLG+  SI++C  MI +VDSDGDG+VNFEEF++MM
Sbjct: 123 SATELCQVLNRLGMRCSIDECHSMIKSVDSDGDGDVNFEEFKRMM 165

[Close]
BLAST of CmoCh10G003510 vs. TrEMBL
Match: A0A0R0K5I7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-44
Identity = 99/175 (56.57%), Postives = 129/175 (73.71%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  KPS  L + EEL++VF RFD+N DG+IS+TELD+VL+SLGS
Sbjct: 39  TNPIEAGNGDAAPNPNATTKPSVYLQDTEELKRVFSRFDANCDGKISVTELDNVLRSLGS 98

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P E+++ VM+DLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYD D NG I
Sbjct: 99  GVPPEDIQRVMDDLDTDHDGFINLSEFAAFCRSDTA--DGGDAELHDAFNLYDHDKNGHI 158

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 159 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 210

[Close]
BLAST of CmoCh10G003510 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 181.8 bits (460), Expect = 3.9e-46
Identity = 94/168 (55.95%), Postives = 124/168 (73.81%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  V+E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmoCh10G003510 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 166.4 bits (420), Expect = 1.7e-41
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  V++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of CmoCh10G003510 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 144.4 bits (363), Expect = 6.8e-35
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmoCh10G003510 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.1e-31
Identity = 66/142 (46.48%), Postives = 97/142 (68.31%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           ++++KVF RFD NGDG+IS+ EL  V+++L      EE   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 91  AFCKEPMA---SDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMI 150
           A  +  +    ++   +S+L++AF+LYD D NG IS  ELH V+  LG   S++DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 151 HTVDSDGDGNVNFEEFRKMMSD 170
             VD DGDG VNF+EF+KMMS+
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMSN 157

[Close]
BLAST of CmoCh10G003510 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 126.3 bits (316), Expect = 1.9e-29
Identity = 66/140 (47.14%), Postives = 89/140 (63.57%), Query Frame = 1 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDE 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P EEL   + ++D   DG+IN +E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
           F     + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of CmoCh10G003510 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-72
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 2.3e-72
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD N I +S SDADQIAK KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL SL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INI+EFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-48
Identity = 105/169 (62.13%), Postives = 133/169 (78.70%), Query Frame = 1 

Query: 9   TSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEE 68
           T+S S+++   KPK S  +   +E++KVF++FD+NGDG+ISITEL+ VL++LGS +P EE
Sbjct: 3   TTSPSESE---KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLETLGSEVPQEE 62

Query: 69  LRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELH 128
           L  VM+DLD D+DGFI +DEFAAFC+    +++A  SELRDAFDLYD+DHNGLIS  ELH
Sbjct: 63  LHRVMDDLDTDRDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEELH 122

Query: 129 LVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           LVL RLG+N S EDC+RMI +VDSDGDGNVNF EF+KMM  TNK    N
Sbjct: 123 LVLNRLGMNCSPEDCRRMIRSVDSDGDGNVNFAEFQKMM--TNKGSNGN 164

[Close]
BLAST of CmoCh10G003510 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 195.3 bits (495), Expect = 9.6e-47
Identity = 97/169 (57.40%), Postives = 128/169 (75.74%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  VM+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmoCh10G003510 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-46
Identity = 103/175 (58.86%), Postives = 132/175 (75.43%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VMEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

The following BLAST results are available for this feature:
BLAST of CmoCh10G003510 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH6.9e-4555.95Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU5.4e-4260.00Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH3.0e-4056.08Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
CML23_ARATH1.2e-3352.17Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ1.3e-3249.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of CmoCh10G003510 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA9.3e-7377.13Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO6.7e-4757.40Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN1.1e-4658.86Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
V7CQX7_PHAVU1.1e-4458.18Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1[more]
A0A0R0K5I7_SOYBN1.4e-4456.57Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1[more]
BLAST of CmoCh10G003510 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.13.9e-4655.95 Calcium-binding EF-hand family protein[more]
AT1G73630.11.7e-4156.08 EF hand calcium-binding protein family[more]
AT1G66400.16.8e-3552.17 calmodulin like 23[more]
AT5G37770.12.1e-3146.48 EF hand calcium-binding protein family[more]
AT1G24620.11.9e-2947.14 EF hand calcium-binding protein family[more]
BLAST of CmoCh10G003510 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|1.3e-7277.13PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|2.3e-7277.13PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|1009165393|ref|XP_015901010.1|1.0e-4862.13PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|14589311|emb|CAC43238.1|9.6e-4757.40calcium binding protein [Sesbania rostrata][more]
gi|356524678|ref|XP_003530955.1|1.6e-4658.86PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh10G003510.1CmoCh10G003510.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 31..94
score: 3.1E-13coord: 105..167
score: 3.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 32..60
score: 1.1E-4coord: 142..170
score: 8.9E-6coord: 68..96
score: 0.0017coord: 106..134
score: 9.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 64..99
score: 10.608coord: 102..137
score: 13.509coord: 28..63
score: 15.071coord: 138..173
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..181
score: 1.7
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 30..179
score: 3.66
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
score: -coord: 41..53
score: -coord: 151..163
score: -coord: 115..127
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 27..172
score: 5.8
NoneNo IPR availablePANTHERPTHR10891:SF573CALCIUM-BINDING PROTEIN CML24-RELATEDcoord: 27..172
score: 5.8

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh10G003510Cucsa.111510Cucumber (Gy14) v1cgycmoB0255
CmoCh10G003510CmaCh10G003260Cucurbita maxima (Rimu)cmacmoB067
CmoCh10G003510CmaCh11G003070Cucurbita maxima (Rimu)cmacmoB099
CmoCh10G003510Cla018929Watermelon (97103) v1cmowmB063
CmoCh10G003510Csa1G025020Cucumber (Chinese Long) v2cmocuB039
CmoCh10G003510MELO3C017353Melon (DHL92) v3.5.1cmomeB059
CmoCh10G003510ClCG06G014370Watermelon (Charleston Gray)cmowcgB064
CmoCh10G003510CSPI01G04290Wild cucumber (PI 183967)cmocpiB042
CmoCh10G003510Lsi06G012900Bottle gourd (USVL1VR-Ls)cmolsiB061
CmoCh10G003510Cp4.1LG18g06750Cucurbita pepo (Zucchini)cmocpeB055
CmoCh10G003510Cp4.1LG04g13590Cucurbita pepo (Zucchini)cmocpeB068
CmoCh10G003510MELO3C017353.2Melon (DHL92) v3.6.1cmomedB062
CmoCh10G003510CsaV3_1G004290Cucumber (Chinese Long) v3cmocucB0046
CmoCh10G003510Cla97C06G123830Watermelon (97103) v2cmowmbB070
CmoCh10G003510Bhi02G000096Wax gourdcmowgoB0071
CmoCh10G003510CsGy1G004180Cucumber (Gy14) v2cgybcmoB007
CmoCh10G003510Carg15175Silver-seed gourdcarcmoB1063
CmoCh10G003510Carg09105Silver-seed gourdcarcmoB0806
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh10G003510CmoCh11G003140Cucurbita moschata (Rifu)cmocmoB038
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh10G003510Cucumber (Chinese Long) v3cmocucB0052
CmoCh10G003510Watermelon (97103) v2cmowmbB060
CmoCh10G003510Wax gourdcmowgoB0057
CmoCh10G003510Cucurbita moschata (Rifu)cmocmoB055
CmoCh10G003510Cucurbita moschata (Rifu)cmocmoB064
CmoCh10G003510Cucumber (Gy14) v1cgycmoB0312
CmoCh10G003510Cucurbita maxima (Rimu)cmacmoB211
CmoCh10G003510Wild cucumber (PI 183967)cmocpiB049
CmoCh10G003510Cucumber (Chinese Long) v2cmocuB046
CmoCh10G003510Melon (DHL92) v3.5.1cmomeB044
CmoCh10G003510Watermelon (Charleston Gray)cmowcgB058
CmoCh10G003510Watermelon (97103) v1cmowmB057
CmoCh10G003510Cucurbita pepo (Zucchini)cmocpeB047
CmoCh10G003510Bottle gourd (USVL1VR-Ls)cmolsiB040
CmoCh10G003510Melon (DHL92) v3.6.1cmomedB048