Carg09105 (gene) Silver-seed gourd

Overview
NameCarg09105
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding EF-hand family protein
LocationCucurbita_argyrosperma_scaffold_063 : 583868 .. 584428 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAACCAGATTACGGCATCAAACGCCGATACCGAGCAGGTCTTGAAGTCCAAGCCCTCCTTCCTTCTCCAGGACAACGCTGAACTCCGCAAGGTTTTCGATCGCTTCGATGCCAATGGCGACGGGAAGATCTCCTTATCCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCATCCGTCCCCTACGACGAGCTCCGCGCCGTCATGGAGGATCTCGACACCGATAAGGACGGTTTCATCAATATCGACGAGTTTGCCGTCTTCTGTAAGGAGCCGATGGCCTCTAATGAAGCCGGAGCGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCATAACGGCCTGATCTCGCAATCTGAGCTCCACCTCGTCCTCAATCGCCTCGGAATCACATGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGATGGAAATGTTAATTTCGAGGAGTTTAGGAAGATGATGTCCAATAATTCCAACTCCGAGGCGCCGAATCAGAATGGAACTGCCGCCACGGCGCCTTAG

mRNA sequence

ATGGCCACTGACTCGAACCAGATTACGGCATCAAACGCCGATACCGAGCAGGTCTTGAAGTCCAAGCCCTCCTTCCTTCTCCAGGACAACGCTGAACTCCGCAAGGTTTTCGATCGCTTCGATGCCAATGGCGACGGGAAGATCTCCTTATCCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCATCCGTCCCCTACGACGAGCTCCGCGCCGTCATGGAGGATCTCGACACCGATAAGGACGGTTTCATCAATATCGACGAGTTTGCCGTCTTCTGTAAGGAGCCGATGGCCTCTAATGAAGCCGGAGCGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCATAACGGCCTGATCTCGCAATCTGAGCTCCACCTCGTCCTCAATCGCCTCGGAATCACATGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGATGGAAATGTTAATTTCGAGGAGTTTAGGAAGATGATGTCCAATAATTCCAACTCCGAGGCGCCGAATCAGAATGGAACTGCCGCCACGGCGCCTTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAACCAGATTACGGCATCAAACGCCGATACCGAGCAGGTCTTGAAGTCCAAGCCCTCCTTCCTTCTCCAGGACAACGCTGAACTCCGCAAGGTTTTCGATCGCTTCGATGCCAATGGCGACGGGAAGATCTCCTTATCCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCATCCGTCCCCTACGACGAGCTCCGCGCCGTCATGGAGGATCTCGACACCGATAAGGACGGTTTCATCAATATCGACGAGTTTGCCGTCTTCTGTAAGGAGCCGATGGCCTCTAATGAAGCCGGAGCGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCATAACGGCCTGATCTCGCAATCTGAGCTCCACCTCGTCCTCAATCGCCTCGGAATCACATGCTCCAAGGAGGACTGTCAACGGATGATCCACTCCGTCGATTCCGATGGCGATGGAAATGTTAATTTCGAGGAGTTTAGGAAGATGATGTCCAATAATTCCAACTCCGAGGCGCCGAATCAGAATGGAACTGCCGCCACGGCGCCTTAG

Protein sequence

MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNGTAATAP
Homology
[Close]
BLAST of Carg09105 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata])

HSP 1 Score: 365.2 bits (936), Expect = 1.4e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180
           LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAATAP 187
           TAATAP
Sbjct: 181 TAATAP 186

[Close]
BLAST of Carg09105 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.1 bits (928), Expect = 1.2e-96
Identity = 184/186 (98.92%), Postives = 185/186 (99.46%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MATDSNQI ASN+DTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL
Sbjct: 1   MATDSNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180
           LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAATAP 187
           TAATAP
Sbjct: 181 TAATAP 186

[Close]
BLAST of Carg09105 vs. NCBI nr
Match: XP_023003784.1 (probable calcium-binding protein CML27 [Cucurbita maxima])

HSP 1 Score: 354.4 bits (908), Expect = 2.4e-94
Identity = 180/186 (96.77%), Postives = 182/186 (97.85%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MATD NQI ASN+DTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKS 
Sbjct: 1   MATDLNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSS 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEP+ASNEAGASELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPIASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180
           LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSE PNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEVPNQNG 180

Query: 181 TAATAP 187
           TAATAP
Sbjct: 181 TAATAP 186

[Close]
BLAST of Carg09105 vs. NCBI nr
Match: XP_022921374.1 (probable calcium-binding protein CML27 [Cucurbita moschata])

HSP 1 Score: 251.1 bits (640), Expect = 2.9e-63
Identity = 129/186 (69.35%), Postives = 145/186 (77.96%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MATDSN IT+SN+D +Q+ K KPS LL +N ELRKVFDRFD+NGDG+IS++ELDSVLKSL
Sbjct: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GS++PY                       A FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180
           LIS SELHLVL RLGI  S EDCQRMIH+VDSDGDGNVNFEEFRKMMS+ + S+APNQNG
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180

Query: 181 TAATAP 187
           TAA  P
Sbjct: 181 TAAAGP 186

[Close]
BLAST of Carg09105 vs. NCBI nr
Match: XP_022988487.1 (probable calcium-binding protein CML27 [Cucurbita maxima])

HSP 1 Score: 248.4 bits (633), Expect = 1.9e-62
Identity = 128/186 (68.82%), Postives = 144/186 (77.42%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MATDSN I +SN+D +Q+ K KPS LL +N ELRKVFDRFD+NGDG+IS++ELDSVLKSL
Sbjct: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GS++PY                      FA FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180
           LIS SELHLVL RLGI  S EDCQRMIH+VDSDGDGNVNFEEFRKMMS+ + S+APNQN 
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNA 180

Query: 181 TAATAP 187
           TAA  P
Sbjct: 181 TAAAGP 186

[Close]
BLAST of Carg09105 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 183.7 bits (465), Expect = 1.0e-46
Identity = 95/173 (54.91%), Postives = 129/173 (74.57%), Query Frame = 0 

Query: 11  SNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELR 70
           ++A+ E    +  +  + +  EL+KVFD+FD+NGDGKIS+ EL  V K++G+S    EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 71  AVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLV 130
            V+E++DTD+DG+IN+DEF+  C+     + + A+E+RDAFDLYDQD NGLIS SELH V
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQV 121

Query: 131 LNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNGTAA 184
           LNRLG++CS EDC RMI  VD+DGDGNVNFEEF+KMM+++S     N NG+AA
Sbjct: 122 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]
BLAST of Carg09105 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 129.4 bits (324), Expect = 2.3e-30
Identity = 72/138 (52.17%), Postives = 85/138 (61.59%), Query Frame = 0 

Query: 32  ELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFAV 91
           EL+KVFD+FDANGDGKIS+SEL +V KS+G                              
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMG-----XXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 92  FCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIHSVD 151
                         E+R+AFDLYDQ+ NGLIS SE+H VLNRLG+TCS EDC RMI  VD
Sbjct: 80  XXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSN 170
           +DGDGNVNFEEF+KMMS+
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]
BLAST of Carg09105 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 129.0 bits (323), Expect = 3.0e-30
Identity = 67/143 (46.85%), Postives = 89/143 (62.24%), Query Frame = 0 

Query: 32  ELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFAV 91
           EL  VF +FD NGDGKIS  EL +++ SLG  VP +EL   + ++D   DG+IN +EF  
Sbjct: 37  ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVE 96

Query: 92  FCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIHSVD 151
              + M  N+     L+DAF +YD D NG IS  ELH VL  LG  CS  +C++MI  VD
Sbjct: 97  LNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVD 156

Query: 152 SDGDGNVNFEEFRKMMSNNSNSE 175
            DGDG ++FEEF+ MM+  S  +
Sbjct: 157 KDGDGTIDFEEFKIMMTMGSRRD 178

[Close]
BLAST of Carg09105 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 115.9 bits (289), Expect = 2.6e-26
Identity = 60/141 (42.55%), Postives = 90/141 (63.83%), Query Frame = 0 

Query: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88
           D  EL +VF  FD NGDGKI+ +EL    KS+G  VP +E+  ++  +D + DG ++IDE
Sbjct: 2   DRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDE 61

Query: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSK--EDCQRM 148
           F    +E M   +    ++R+AF ++DQ+ +G I+  EL  VL  +G+   +  EDC++M
Sbjct: 62  FGSLYQE-MVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKM 121

Query: 149 IHSVDSDGDGNVNFEEFRKMM 168
           I  VD DGDG VNF+EF++MM
Sbjct: 122 ISKVDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Carg09105 vs. TAIR10
Match: AT3G03400.1 (EF hand calcium-binding protein family)

HSP 1 Score: 102.4 bits (254), Expect = 3.0e-22
Identity = 54/134 (40.30%), Postives = 81/134 (60.45%), Query Frame = 0 

Query: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88
           +N  L  +F+RFD + DGKIS  E    + +L  S+P ++L  +   LDT+ DG ++  +
Sbjct: 2   NNMSLSDIFERFDTSKDGKISWEEFRDAIHALSPSIPSEKLVEMFIQLDTNGDGQVDAAK 61

Query: 89  FAVFCKEPMASNEAG--ASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRM 148
           FA  C +  A +  G    EL+DAF LYD + +G IS +ELH+V+ RLG  C+ E C  M
Sbjct: 62  FA-SCMDQTAQSSGGDVEKELKDAFKLYDINCDGKISANELHVVMTRLGEKCTVESCVGM 121

Query: 149 IHSVDSDGDGNVNF 161
           + ++D DGDG + F
Sbjct: 122 VQAIDVDGDGYIRF 134

[Close]
BLAST of Carg09105 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.8e-45
Identity = 95/173 (54.91%), Postives = 129/173 (74.57%), Query Frame = 0 

Query: 11  SNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELR 70
           ++A+ E    +  +  + +  EL+KVFD+FD+NGDGKIS+ EL  V K++G+S    EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 71  AVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLV 130
            V+E++DTD+DG+IN+DEF+  C+     + + A+E+RDAFDLYDQD NGLIS SELH V
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQV 121

Query: 131 LNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNGTAA 184
           LNRLG++CS EDC RMI  VD+DGDGNVNFEEF+KMM+++S     N NG+AA
Sbjct: 122 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS---LLNSNGSAA 166

[Close]
BLAST of Carg09105 vs. Swiss-Prot
Match: sp|Q9M7R0|ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.7e-43
Identity = 98/171 (57.31%), Postives = 124/171 (72.51%), Query Frame = 0 

Query: 10  ASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDEL 69
           A+N D      SKPS  LQ+  E++ VF+RFDANGDGKIS  EL  VLK+LGS+   +E+
Sbjct: 2   AANTDR----NSKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEI 61

Query: 70  RAVMEDLDTDKDGFINIDEFAVFCK---EPMASNEAGASELRDAFDLYDQDHNGLISQSE 129
             +ME++DTDKDGFIN+ EFA F K   +P  S+  G +EL++AF+LYDQDHNGLIS  E
Sbjct: 62  GRIMEEIDTDKDGFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVE 121

Query: 130 LHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNS--NSEA 176
           LH +L RLG   ++ DC  MI SVDSDGDG V+FEEF+KMM+N S  NS+A
Sbjct: 122 LHKILTRLGERYAEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKSGNNSQA 167

[Close]
BLAST of Carg09105 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.5e-35
Identity = 77/148 (52.03%), Postives = 102/148 (68.92%), Query Frame = 0 

Query: 31  AELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFA 90
           AEL +VF R+DANGDGKIS  EL SVL++LG+ +   E+R +M+++D+D+DGF+++ EFA
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 91  VFCKEPMASN-----------EAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCS 150
            F   P  ++            A  +ELR+AF +YD D NG IS  ELH VL +LG  CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 151 KEDCQRMIHSVDSDGDGNVNFEEFRKMM 168
             DC RMI SVD+DGDG VNF+EF+KMM
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of Carg09105 vs. Swiss-Prot
Match: sp|Q5ZCK5|CML16_ORYSJ (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.3e-30
Identity = 76/160 (47.50%), Postives = 105/160 (65.62%), Query Frame = 0 

Query: 16  EQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL----GSSVPYDELRA 75
           +Q    +P+ L   +AE+ +VF RFDA+GDG+IS SEL +V +++      S    E+ A
Sbjct: 11  QQQQVERPTALAPADAEIERVFTRFDADGDGRISPSELAAVTRAIAPPPSESAGGREVAA 70

Query: 76  VMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVL 135
           +M +LDTD+DGF+++ EFA F        E  A ELR AFD+YD D +G I+ +EL  VL
Sbjct: 71  MMNELDTDRDGFVDLGEFAAFHGRGRGDAEHEA-ELRAAFDVYDVDGDGRITAAELGKVL 130

Query: 136 NRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNS 172
            R+G  CS E+C+RMI SVD DGDG V FEEF+KMM  ++
Sbjct: 131 GRIGEGCSAEECERMIASVDVDGDGCVGFEEFKKMMCRDA 169

[Close]
BLAST of Carg09105 vs. Swiss-Prot
Match: sp|Q9C9U8|CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.1e-29
Identity = 72/138 (52.17%), Postives = 85/138 (61.59%), Query Frame = 0 

Query: 32  ELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFAV 91
           EL+KVFD+FDANGDGKIS+SEL +V KS+G                              
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMG-----XXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 92  FCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIHSVD 151
                         E+R+AFDLYDQ+ NGLIS SE+H VLNRLG+TCS EDC RMI  VD
Sbjct: 80  XXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSN 170
           +DGDGNVNFEEF+KMMS+
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]
BLAST of Carg09105 vs. TrEMBL
Match: tr|A0A1J7G171|A0A1J7G171_LUPAN (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13804 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 9.4e-47
Identity = 96/140 (68.57%), Postives = 115/140 (82.14%), Query Frame = 0 

Query: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88
           D  EL++VF+RFDANGDGKIS++ELD+ L+SLGSSV   E++ +MEDLDTD DGFIN+ E
Sbjct: 24  DAEELKRVFNRFDANGDGKISVTELDTALRSLGSSVSTSEVQCIMEDLDTDHDGFINLSE 83

Query: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148
           FA FC+     ++   SELRDAFDLYDQD NGLIS +ELHLVLNRLG+ CS E+C  MI 
Sbjct: 84  FAAFCR-----SDGDVSELRDAFDLYDQDKNGLISATELHLVLNRLGVKCSVEECHSMIK 143

Query: 149 SVDSDGDGNVNFEEFRKMMS 169
           SVDSDGDGNV+FEEF+KMMS
Sbjct: 144 SVDSDGDGNVSFEEFKKMMS 158

[Close]
BLAST of Carg09105 vs. TrEMBL
Match: tr|A0A2P6P1W9|A0A2P6P1W9_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0178551 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-45
Identity = 98/149 (65.77%), Postives = 118/149 (79.19%), Query Frame = 0 

Query: 32  ELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDEFAV 91
           E+R+VF  FDANGDGKIS SEL +VL++LGS+VP DEL+ VM DLDTD+DGFI +DEF  
Sbjct: 21  EVRRVFKSFDANGDGKISTSELGNVLEALGSNVPEDELQRVMVDLDTDRDGFICLDEFNA 80

Query: 92  FCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIHSVD 151
           F      + + G+SEL++AFDLYDQD NGLIS  ELHLVLNRL + C+ EDC RMI SVD
Sbjct: 81  F----WVAGDGGSSELKEAFDLYDQDRNGLISVDELHLVLNRLQMKCTVEDCNRMIQSVD 140

Query: 152 SDGDGNVNFEEFRKMMSNNSNSEAPNQNG 181
           +DGDGNVNFEEF+KMMS+NS+S     NG
Sbjct: 141 ADGDGNVNFEEFKKMMSHNSSSAGAVSNG 165

[Close]
BLAST of Carg09105 vs. TrEMBL
Match: tr|A0A0K9QUU9|A0A0K9QUU9_SPIOL (Uncharacterized protein OS=Spinacia oleracea OX=3562 GN=SOVF_143520 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.0e-45
Identity = 95/177 (53.67%), Postives = 128/177 (72.32%), Query Frame = 0 

Query: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60
           MAT + +  ++ + +     S  S  L D AE++KVFDRFD NGDGKIS  EL  V+K+L
Sbjct: 1   MATTTTEENSNKSSSH----SLSSVYLSDTAEVKKVFDRFDVNGDGKISAEELIGVMKAL 60

Query: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120
           GS    +E+  +ME++DTD+DGFIN++EFA FC   +  +E+G+ EL DAF +YDQD NG
Sbjct: 61  GSETSPEEVAQMMEEMDTDRDGFINLEEFADFCSRKVEGDESGSQELHDAFKMYDQDENG 120

Query: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPN 178
           LIS +ELHLVL+RLG  CS EDC +MI SVD+DGDGNV++EEFRKMM+   ++ + N
Sbjct: 121 LISAAELHLVLSRLGEGCSVEDCSKMIQSVDADGDGNVSYEEFRKMMTKGKSTSSSN 173

[Close]
BLAST of Carg09105 vs. TrEMBL
Match: tr|A0A1S4BWY5|A0A1S4BWY5_TOBAC (calcium-binding allergen Ole e 8-like OS=Nicotiana tabacum OX=4097 GN=LOC107812761 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.8e-45
Identity = 95/153 (62.09%), Postives = 117/153 (76.47%), Query Frame = 0 

Query: 19  LKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDT 78
           + +KPS  LQD  E++KVF RFDANGDG IS  EL  VLK+LGS    DE+  +M ++DT
Sbjct: 1   MATKPSVYLQDMEEVKKVFARFDANGDGMISGDELSGVLKALGSDTSPDEVERMMAEIDT 60

Query: 79  DKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITC 138
           D+DG IN+ EFA FCK    S + GA EL++AFDLYDQDHNGLIS +ELH +LNRLG  C
Sbjct: 61  DRDGCINLQEFADFCKSD-DSVDGGAKELKEAFDLYDQDHNGLISAAELHQILNRLGQNC 120

Query: 139 SKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNS 172
           + +DC RMI SVD+DGDGNV+FEEF++MM NNS
Sbjct: 121 TIQDCTRMITSVDADGDGNVSFEEFKQMMCNNS 152

[Close]
BLAST of Carg09105 vs. TrEMBL
Match: tr|A0A1U7X6X3|A0A1U7X6X3_NICSY (calcium-binding allergen Ole e 8-like OS=Nicotiana sylvestris OX=4096 GN=LOC104231002 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.8e-45
Identity = 95/153 (62.09%), Postives = 117/153 (76.47%), Query Frame = 0 

Query: 19  LKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDT 78
           + +KPS  LQD  E++KVF RFDANGDG IS  EL  VLK+LGS    DE+  +M ++DT
Sbjct: 1   MATKPSVYLQDMEEVKKVFARFDANGDGMISGDELSGVLKALGSDTSPDEVERMMAEIDT 60

Query: 79  DKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITC 138
           D+DG IN+ EFA FCK    S + GA EL++AFDLYDQDHNGLIS +ELH +LNRLG  C
Sbjct: 61  DRDGCINLQEFADFCKSD-DSVDGGAKELKEAFDLYDQDHNGLISAAELHQILNRLGQNC 120

Query: 139 SKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNS 172
           + +DC RMI SVD+DGDGNV+FEEF++MM NNS
Sbjct: 121 TIQDCTRMITSVDADGDGNVSFEEFKQMMCNNS 152

The following BLAST results are available for this feature:
BLAST of Carg09105 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022933917.11.4e-97100.00probable calcium-binding protein CML27 [Cucurbita moschata][more]
XP_023529417.11.2e-9698.92probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo][more]
XP_023003784.12.4e-9496.77probable calcium-binding protein CML27 [Cucurbita maxima][more]
XP_022921374.12.9e-6369.35probable calcium-binding protein CML27 [Cucurbita moschata][more]
XP_022988487.11.9e-6268.82probable calcium-binding protein CML27 [Cucurbita maxima][more]
BLAST of Carg09105 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.11.0e-4654.91Calcium-binding EF-hand family protein[more]
AT1G73630.12.3e-3052.17EF hand calcium-binding protein family[more]
AT1G24620.13.0e-3046.85EF hand calcium-binding protein family[more]
AT4G12860.12.6e-2642.55EF hand calcium-binding protein family[more]
AT3G03400.13.0e-2240.30EF hand calcium-binding protein family[more]
BLAST of Carg09105 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9LE22|CML27_ARATH1.8e-4554.91Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
sp|Q9M7R0|ALL8_OLEEU1.7e-4357.31Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1[more]
sp|Q0DJV6|CML18_ORYSJ6.5e-3552.03Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q5ZCK5|CML16_ORYSJ1.3e-3047.50Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9C9U8|CML26_ARATH4.1e-2952.17Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ... [more]
BLAST of Carg09105 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1J7G171|A0A1J7G171_LUPAN9.4e-4768.57Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13804 PE=4 S... [more]
tr|A0A2P6P1W9|A0A2P6P1W9_ROSCH2.3e-4565.77Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7... [more]
tr|A0A0K9QUU9|A0A0K9QUU9_SPIOL4.0e-4553.67Uncharacterized protein OS=Spinacia oleracea OX=3562 GN=SOVF_143520 PE=4 SV=1[more]
tr|A0A1S4BWY5|A0A1S4BWY5_TOBAC6.8e-4562.09calcium-binding allergen Ole e 8-like OS=Nicotiana tabacum OX=4097 GN=LOC1078127... [more]
tr|A0A1U7X6X3|A0A1U7X6X3_NICSY6.8e-4562.09calcium-binding allergen Ole e 8-like OS=Nicotiana sylvestris OX=4096 GN=LOC1042... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09105-RACarg09105-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 32..60
e-value: 2.6E-6
score: 37.0
coord: 106..134
e-value: 2.3E-4
score: 30.5
coord: 142..170
e-value: 1.2E-4
score: 31.5
coord: 68..96
e-value: 7.4E-5
score: 32.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 32..93
e-value: 1.1E-13
score: 51.4
coord: 105..167
e-value: 6.1E-14
score: 52.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 102..137
score: 12.784
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 28..63
score: 15.462
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 64..99
score: 12.114
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 138..173
score: 13.621
IPR002048EF-hand domainCDDcd00051EFhcoord: 106..168
e-value: 4.4224E-20
score: 79.5141
IPR002048EF-hand domainCDDcd00051EFhcoord: 32..90
e-value: 3.17612E-17
score: 71.4249
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 102..184
e-value: 2.9E-24
score: 86.9
coord: 3..101
e-value: 1.0E-21
score: 78.7
NoneNo IPR availablePANTHERPTHR10891:SF737SUBFAMILY NOT NAMEDcoord: 20..175
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 20..175
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 151..163
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 41..53
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 115..127
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 30..178

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg09105Cla018929Watermelon (97103) v1carwmB0594
Carg09105Csa1G025020Cucumber (Chinese Long) v2carcuB0622
Carg09105MELO3C017353Melon (DHL92) v3.5.1carmeB0599
Carg09105ClCG06G014370Watermelon (Charleston Gray)carwcgB0581
Carg09105CSPI01G04290Wild cucumber (PI 183967)carcpiB0628
Carg09105Cucsa.111510Cucumber (Gy14) v1carcgyB0668
Carg09105CmaCh11G003070Cucurbita maxima (Rimu)carcmaB0830
Carg09105CmaCh10G003260Cucurbita maxima (Rimu)carcmaB0828
Carg09105CmoCh11G003140Cucurbita moschata (Rifu)carcmoB0808
Carg09105CmoCh10G003510Cucurbita moschata (Rifu)carcmoB0806
Carg09105Lsi06G012900Bottle gourd (USVL1VR-Ls)carlsiB540
Carg09105Cp4.1LG18g06750Cucurbita pepo (Zucchini)carcpeB0843
Carg09105Cp4.1LG04g13590Cucurbita pepo (Zucchini)carcpeB0847
Carg09105CsGy1G004180Cucumber (Gy14) v2carcgybB0594
Carg09105MELO3C017353.2Melon (DHL92) v3.6.1carmedB0606
Carg09105CsaV3_1G004290Cucumber (Chinese Long) v3carcucB0629
Carg09105Cla97C06G123830Watermelon (97103) v2carwmbB0623
Carg09105Bhi02G000096Wax gourdcarwgoB0710
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg09105Carg15175Silver-seed gourdcarcarB537
The following block(s) are covering this gene:
GeneOrganismBlock
Carg09105Cucurbita maxima (Rimu)carcmaB0832
Carg09105Cucurbita maxima (Rimu)carcmaB0834
Carg09105Cucurbita moschata (Rifu)carcmoB0810
Carg09105Cucurbita moschata (Rifu)carcmoB0812
Carg09105Cucurbita pepo (Zucchini)carcpeB0840
Carg09105Cucurbita pepo (Zucchini)carcpeB0846
Carg09105Wild cucumber (PI 183967)carcpiB0629
Carg09105Cucumber (Chinese Long) v2carcuB0623
Carg09105Cucumber (Chinese Long) v3carcucB0630
Carg09105Bottle gourd (USVL1VR-Ls)carlsiB538
Carg09105Melon (DHL92) v3.5.1carmeB0598
Carg09105Melon (DHL92) v3.6.1carmedB0605
Carg09105Watermelon (Charleston Gray)carwcgB0579
Carg09105Watermelon (97103) v1carwmB0592
Carg09105Watermelon (97103) v2carwmbB0621
Carg09105Wax gourdcarwgoB0705
Carg09105Silver-seed gourdcarcarB416
Carg09105Cucumber (Gy14) v2carcgybB0595
Carg09105Cucumber (Gy14) v1carcgyB0670