CmaCh10G003260 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh10G003260
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium binding protein
LocationCma_Chr10 : 1505583 .. 1506143 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

Protein sequence

MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAAAGP
Homology
[Close]
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.2e-43
Identity = 91/168 (54.17%), Postives = 122/168 (72.62%), Query Frame = 1 

Query: 17  QITKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           +  KP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N N +AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.6e-41
Identity = 93/159 (58.49%), Postives = 116/159 (72.96%), Query Frame = 1 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKD 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS    EE+  IME++D DKD
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  GFINIDEFAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
           GFIN+ EFAAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSD--ANKSKA 176
           +  DC  MI +VDSDGDG V+FEEF+KMM++   N S+A
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKSGNNSQA 167

[Close]
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.9e-40
Identity = 82/149 (55.03%), Postives = 110/149 (73.83%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDANKSKAPNQNA 181
           +DGDGNVNFEEF+KMMS     K    N+
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVANS 163

[Close]
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-33
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-32
Identity = 73/147 (49.66%), Postives = 101/147 (68.71%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +   E+R +M+++D D+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of CmaCh10G003260 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 6.0e-72
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPIP+S S+ADQI K  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.7e-47
Identity = 96/171 (56.14%), Postives = 131/171 (76.61%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MAT+     ++ ++ +  TK KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  GSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NG
Sbjct: 61  GSTVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDAN 172
           LIS +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 121 LISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmaCh10G003260 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.7e-46
Identity = 101/175 (57.71%), Postives = 131/175 (74.86%), Query Frame = 1 

Query: 5   SNPIPSSNSDA--DQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +     K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

[Close]
BLAST of CmaCh10G003260 vs. TrEMBL
Match: G7LDC5_MEDTR (EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_8g107110 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.1e-44
Identity = 92/160 (57.50%), Postives = 124/160 (77.50%), Query Frame = 1 

Query: 10  SSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEEL 69
           ++N + +  T  K S  L + +EL+ VF RFD+NGDG+IS+TELD++L+SLGS +P +EL
Sbjct: 2   ATNPNTETTTTTKSSVYLGDMDELKTVFTRFDTNGDGKISVTELDNILRSLGSTVPKDEL 61

Query: 70  RGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHL 129
           + +MEDLD D+DGFIN+ EFAAFC+    S +  +SELR+AFDLYD+D NGLIS +EL  
Sbjct: 62  QRVMEDLDTDRDGFINLAEFAAFCRS--GSADGDVSELREAFDLYDKDKNGLISATELCQ 121

Query: 130 VLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSD 170
           VL  LG+  S+E+C  MI +VDSDGDGNVNFEEF+KMM++
Sbjct: 122 VLNTLGMKCSVEECHTMIKSVDSDGDGNVNFEEFKKMMNN 159

[Close]
BLAST of CmaCh10G003260 vs. TrEMBL
Match: A0A061FWP8_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.1e-44
Identity = 94/150 (62.67%), Postives = 118/150 (78.67%), Query Frame = 1 

Query: 20  KPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDY 79
           +P  S +  EN +EL+KVF++FD+N DG+IS++EL  VLK++GS+   EEL  +MED+D 
Sbjct: 9   QPNTSSVYLENMDELQKVFNQFDANKDGKISVSELGDVLKAMGSSYSKEELERVMEDIDT 68

Query: 80  DKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 139
           DKDGFIN+ EF+AFC+    +  A  SELRDAFDLYDQD NGLIS +ELHLVL RLG+  
Sbjct: 69  DKDGFINLSEFSAFCRSSSDAVSAA-SELRDAFDLYDQDKNGLISANELHLVLNRLGLKC 128

Query: 140 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMS 169
           S++DC RMI +VDSDGDGNVNFEEF KMMS
Sbjct: 129 SVDDCVRMITSVDSDGDGNVNFEEFEKMMS 157

[Close]
BLAST of CmaCh10G003260 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 91/168 (54.17%), Postives = 122/168 (72.62%), Query Frame = 1 

Query: 17  QITKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           +  KP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N N +AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmaCh10G003260 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 166.0 bits (419), Expect = 2.2e-41
Identity = 82/149 (55.03%), Postives = 110/149 (73.83%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDANKSKAPNQNA 181
           +DGDGNVNFEEF+KMMS     K    N+
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVANS 163

[Close]
BLAST of CmaCh10G003260 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 144.1 bits (362), Expect = 8.9e-35
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmaCh10G003260 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.1e-31
Identity = 66/142 (46.48%), Postives = 97/142 (68.31%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           ++++KVF RFD NGDG+IS+ EL  V+++L      EE   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 91  AFCKEPMA---SDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMI 150
           A  +  +    ++   +S+L++AF+LYD D NG IS  ELH V+  LG   S++DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 151 HTVDSDGDGNVNFEEFRKMMSD 170
             VD DGDG VNF+EF+KMMS+
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMSN 157

[Close]
BLAST of CmaCh10G003260 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 125.9 bits (315), Expect = 2.5e-29
Identity = 66/140 (47.14%), Postives = 89/140 (63.57%), Query Frame = 1 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDE 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P EEL   + ++D   DG+IN +E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
           F     + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of CmaCh10G003260 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 8.6e-72
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPIP+S S+ADQI K  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-71
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD N IP+S SDADQI K KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL SL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INI+EFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 201.1 bits (510), Expect = 1.7e-48
Identity = 97/150 (64.67%), Postives = 124/150 (82.67%), Query Frame = 1 

Query: 20  KPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYD 79
           KPK S  +   +E++KVF++FD+NGDG+ISITEL+ VL++LGS +P EEL  +M+DLD D
Sbjct: 11  KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLETLGSEVPQEELHRVMDDLDTD 70

Query: 80  KDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYS 139
           +DGFI +DEFAAFC+    +++A  SELRDAFDLYD+DHNGLIS  ELHLVL RLG+N S
Sbjct: 71  RDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEELHLVLNRLGMNCS 130

Query: 140 IEDCQRMIHTVDSDGDGNVNFEEFRKMMSD 170
            EDC+RMI +VDSDGDGNVNF EF+KMM++
Sbjct: 131 PEDCRRMIRSVDSDGDGNVNFAEFQKMMTN 158

[Close]
BLAST of CmaCh10G003260 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 195.3 bits (495), Expect = 9.6e-47
Identity = 96/171 (56.14%), Postives = 131/171 (76.61%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MAT+     ++ ++ +  TK KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  GSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NG
Sbjct: 61  GSTVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDAN 172
           LIS +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 121 LISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmaCh10G003260 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 192.2 bits (487), Expect = 8.1e-46
Identity = 101/175 (57.71%), Postives = 131/175 (74.86%), Query Frame = 1 

Query: 5   SNPIPSSNSDA--DQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +     K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

The following BLAST results are available for this feature:
BLAST of CmaCh10G003260 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH2.2e-4354.17Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU1.6e-4158.49Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH3.9e-4055.03Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
CML23_ARATH1.6e-3352.17Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ1.3e-3249.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of CmaCh10G003260 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA6.0e-7276.06Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO6.7e-4756.14Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN5.7e-4657.71Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
G7LDC5_MEDTR3.1e-4457.50EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_8g107110 PE... [more]
A0A061FWP8_THECC4.1e-4462.67Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=... [more]
BLAST of CmaCh10G003260 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.11.2e-4454.17 Calcium-binding EF-hand family protein[more]
AT1G73630.12.2e-4155.03 EF hand calcium-binding protein family[more]
AT1G66400.18.9e-3552.17 calmodulin like 23[more]
AT5G37770.12.1e-3146.48 EF hand calcium-binding protein family[more]
AT1G24620.12.5e-2947.14 EF hand calcium-binding protein family[more]
BLAST of CmaCh10G003260 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|8.6e-7276.06PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|1.5e-7176.06PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|1009165393|ref|XP_015901010.1|1.7e-4864.67PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|14589311|emb|CAC43238.1|9.6e-4756.14calcium binding protein [Sesbania rostrata][more]
gi|356524678|ref|XP_003530955.1|8.1e-4657.71PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G003260.1CmaCh10G003260.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 31..94
score: 2.8E-13coord: 105..167
score: 3.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 142..170
score: 2.6E-5coord: 32..60
score: 1.1E-4coord: 68..96
score: 8.3E-4coord: 106..134
score: 9.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 64..99
score: 10.831coord: 102..137
score: 13.509coord: 28..63
score: 15.071coord: 138..173
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..180
score: 2.3
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 30..174
score: 6.13
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
score: -coord: 115..127
score: -coord: 151..163
score: -coord: 41..53
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 27..172
score: 5.6
NoneNo IPR availablePANTHERPTHR10891:SF573CALCIUM-BINDING PROTEIN CML24-RELATEDcoord: 27..172
score: 5.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh10G003260Cucsa.111510Cucumber (Gy14) v1cgycmaB0256
CmaCh10G003260Cla018929Watermelon (97103) v1cmawmB071
CmaCh10G003260Csa1G025020Cucumber (Chinese Long) v2cmacuB050
CmaCh10G003260MELO3C017353Melon (DHL92) v3.5.1cmameB067
CmaCh10G003260ClCG06G014370Watermelon (Charleston Gray)cmawcgB071
CmaCh10G003260CSPI01G04290Wild cucumber (PI 183967)cmacpiB051
CmaCh10G003260CmoCh11G003140Cucurbita moschata (Rifu)cmacmoB068
CmaCh10G003260CmoCh10G003510Cucurbita moschata (Rifu)cmacmoB067
CmaCh10G003260Lsi06G012900Bottle gourd (USVL1VR-Ls)cmalsiB075
CmaCh10G003260Cp4.1LG18g06750Cucurbita pepo (Zucchini)cmacpeB080
CmaCh10G003260Cp4.1LG04g13590Cucurbita pepo (Zucchini)cmacpeB092
CmaCh10G003260MELO3C017353.2Melon (DHL92) v3.6.1cmamedB070
CmaCh10G003260CsaV3_1G004290Cucumber (Chinese Long) v3cmacucB0060
CmaCh10G003260Cla97C06G123830Watermelon (97103) v2cmawmbB081
CmaCh10G003260Bhi02G000096Wax gourdcmawgoB0077
CmaCh10G003260CsGy1G004180Cucumber (Gy14) v2cgybcmaB006
CmaCh10G003260Carg15175Silver-seed gourdcarcmaB1091
CmaCh10G003260Carg09105Silver-seed gourdcarcmaB0828
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh10G003260CmaCh11G003070Cucurbita maxima (Rimu)cmacmaB072
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh10G003260Cucurbita pepo (Zucchini)cmacpeB073
CmaCh10G003260Melon (DHL92) v3.6.1cmamedB056
CmaCh10G003260Cucumber (Chinese Long) v3cmacucB0065
CmaCh10G003260Watermelon (97103) v2cmawmbB071
CmaCh10G003260Cucurbita maxima (Rimu)cmacmaB093
CmaCh10G003260Cucurbita maxima (Rimu)cmacmaB100
CmaCh10G003260Cucurbita maxima (Rimu)cmacmaB103
CmaCh10G003260Cucumber (Gy14) v1cgycmaB0315
CmaCh10G003260Wild cucumber (PI 183967)cmacpiB057
CmaCh10G003260Cucumber (Chinese Long) v2cmacuB056
CmaCh10G003260Melon (DHL92) v3.5.1cmameB054
CmaCh10G003260Watermelon (Charleston Gray)cmawcgB065
CmaCh10G003260Watermelon (97103) v1cmawmB064