CmaCh10G003260.1 (mRNA) Cucurbita maxima (Rimu)

Overview
NameCmaCh10G003260.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium binding protein
LocationCma_Chr10 : 1505583 .. 1506143 (-)
Sequence length561
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTCCGTCCTCTAACTCCGACGCCGACCAGATCACCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCATCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGATCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATGCTAACAAATCCAAGGCGCCGAATCAAAATGCAACTGCAGCCGCGGGGCCCTAG

Protein sequence

MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAAAGP
Homology
[Close]
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.2e-43
Identity = 91/168 (54.17%), Postives = 122/168 (72.62%), Query Frame = 1 

Query: 17  QITKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           +  KP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N N +AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.6e-41
Identity = 93/159 (58.49%), Postives = 116/159 (72.96%), Query Frame = 1 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKD 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS    EE+  IME++D DKD
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  GFINIDEFAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
           GFIN+ EFAAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSD--ANKSKA 176
           +  DC  MI +VDSDGDG V+FEEF+KMM++   N S+A
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMMTNKSGNNSQA 167

[Close]
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.9e-40
Identity = 82/149 (55.03%), Postives = 110/149 (73.83%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDANKSKAPNQNA 181
           +DGDGNVNFEEF+KMMS     K    N+
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVANS 163

[Close]
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-33
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-32
Identity = 73/147 (49.66%), Postives = 101/147 (68.71%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +   E+R +M+++D D+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 6.0e-72
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPIP+S S+ADQI K  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.7e-47
Identity = 96/171 (56.14%), Postives = 131/171 (76.61%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MAT+     ++ ++ +  TK KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  GSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NG
Sbjct: 61  GSTVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDAN 172
           LIS +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 121 LISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.7e-46
Identity = 101/175 (57.71%), Postives = 131/175 (74.86%), Query Frame = 1 

Query: 5   SNPIPSSNSDA--DQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +     K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

[Close]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match: G7LDC5_MEDTR (EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_8g107110 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.1e-44
Identity = 92/160 (57.50%), Postives = 124/160 (77.50%), Query Frame = 1 

Query: 10  SSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEEL 69
           ++N + +  T  K S  L + +EL+ VF RFD+NGDG+IS+TELD++L+SLGS +P +EL
Sbjct: 2   ATNPNTETTTTTKSSVYLGDMDELKTVFTRFDTNGDGKISVTELDNILRSLGSTVPKDEL 61

Query: 70  RGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHL 129
           + +MEDLD D+DGFIN+ EFAAFC+    S +  +SELR+AFDLYD+D NGLIS +EL  
Sbjct: 62  QRVMEDLDTDRDGFINLAEFAAFCRS--GSADGDVSELREAFDLYDKDKNGLISATELCQ 121

Query: 130 VLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSD 170
           VL  LG+  S+E+C  MI +VDSDGDGNVNFEEF+KMM++
Sbjct: 122 VLNTLGMKCSVEECHTMIKSVDSDGDGNVNFEEFKKMMNN 159

[Close]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match: A0A061FWP8_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.1e-44
Identity = 94/150 (62.67%), Postives = 118/150 (78.67%), Query Frame = 1 

Query: 20  KPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDY 79
           +P  S +  EN +EL+KVF++FD+N DG+IS++EL  VLK++GS+   EEL  +MED+D 
Sbjct: 9   QPNTSSVYLENMDELQKVFNQFDANKDGKISVSELGDVLKAMGSSYSKEELERVMEDIDT 68

Query: 80  DKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 139
           DKDGFIN+ EF+AFC+    +  A  SELRDAFDLYDQD NGLIS +ELHLVL RLG+  
Sbjct: 69  DKDGFINLSEFSAFCRSSSDAVSAA-SELRDAFDLYDQDKNGLISANELHLVLNRLGLKC 128

Query: 140 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMS 169
           S++DC RMI +VDSDGDGNVNFEEF KMMS
Sbjct: 129 SVDDCVRMITSVDSDGDGNVNFEEFEKMMS 157

[Close]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 91/168 (54.17%), Postives = 122/168 (72.62%), Query Frame = 1 

Query: 17  QITKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMED 76
           +  KP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  ++E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNATAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N N +AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 166.0 bits (419), Expect = 2.2e-41
Identity = 82/149 (55.03%), Postives = 110/149 (73.83%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  +++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDANKSKAPNQNA 181
           +DGDGNVNFEEF+KMMS     K    N+
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVANS 163

[Close]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 144.1 bits (362), Expect = 8.9e-35
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.1e-31
Identity = 66/142 (46.48%), Postives = 97/142 (68.31%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDEFA 90
           ++++KVF RFD NGDG+IS+ EL  V+++L      EE   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 91  AFCKEPMA---SDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMI 150
           A  +  +    ++   +S+L++AF+LYD D NG IS  ELH V+  LG   S++DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 151 HTVDSDGDGNVNFEEFRKMMSD 170
             VD DGDG VNF+EF+KMMS+
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMSN 157

[Close]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 125.9 bits (315), Expect = 2.5e-29
Identity = 66/140 (47.14%), Postives = 89/140 (63.57%), Query Frame = 1 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDE 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P EEL   + ++D   DG+IN +E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
           F     + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 8.6e-72
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPIP+S S+ADQI K  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-71
Identity = 143/188 (76.06%), Postives = 163/188 (86.70%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD N IP+S SDADQI K KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL SL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  G--SAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR +M+DLD DKDG+INI+EFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NATAAAGP 187
           N TAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 201.1 bits (510), Expect = 1.7e-48
Identity = 97/150 (64.67%), Postives = 124/150 (82.67%), Query Frame = 1 

Query: 20  KPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYD 79
           KPK S  +   +E++KVF++FD+NGDG+ISITEL+ VL++LGS +P EEL  +M+DLD D
Sbjct: 11  KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLETLGSEVPQEELHRVMDDLDTD 70

Query: 80  KDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYS 139
           +DGFI +DEFAAFC+    +++A  SELRDAFDLYD+DHNGLIS  ELHLVL RLG+N S
Sbjct: 71  RDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEELHLVLNRLGMNCS 130

Query: 140 IEDCQRMIHTVDSDGDGNVNFEEFRKMMSD 170
            EDC+RMI +VDSDGDGNVNF EF+KMM++
Sbjct: 131 PEDCRRMIRSVDSDGDGNVNFAEFQKMMTN 158

[Close]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 195.3 bits (495), Expect = 9.6e-47
Identity = 96/171 (56.14%), Postives = 131/171 (76.61%), Query Frame = 1 

Query: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MAT+     ++ ++ +  TK KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++L
Sbjct: 1   MATNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRAL 60

Query: 61  GSAIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS +P +EL  +M+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NG
Sbjct: 61  GSTVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDAN 172
           LIS +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 121 LISAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 192.2 bits (487), Expect = 8.1e-46
Identity = 101/175 (57.71%), Postives = 131/175 (74.86%), Query Frame = 1 

Query: 5   SNPIPSSNSDA--DQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +     K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGIMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ +MEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

The following BLAST results are available for this feature:
BLAST of CmaCh10G003260.1 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH2.2e-4354.17Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU1.6e-4158.49Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH3.9e-4055.03Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
CML23_ARATH1.6e-3352.17Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ1.3e-3249.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of CmaCh10G003260.1 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA6.0e-7276.06Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO6.7e-4756.14Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN5.7e-4657.71Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
G7LDC5_MEDTR3.1e-4457.50EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_8g107110 PE... [more]
A0A061FWP8_THECC4.1e-4462.67Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_012743 PE=4 SV=... [more]
BLAST of CmaCh10G003260.1 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.11.2e-4454.17 Calcium-binding EF-hand family protein[more]
AT1G73630.12.2e-4155.03 EF hand calcium-binding protein family[more]
AT1G66400.18.9e-3552.17 calmodulin like 23[more]
AT5G37770.12.1e-3146.48 EF hand calcium-binding protein family[more]
AT1G24620.12.5e-2947.14 EF hand calcium-binding protein family[more]
BLAST of CmaCh10G003260.1 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|8.6e-7276.06PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|1.5e-7176.06PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|1009165393|ref|XP_015901010.1|1.7e-4864.67PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|14589311|emb|CAC43238.1|9.6e-4756.14calcium binding protein [Sesbania rostrata][more]
gi|356524678|ref|XP_003530955.1|8.1e-4657.71PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh10G003260CmaCh10G003260gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh10G003260.1CmaCh10G003260.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G003260.1.CDS.1CmaCh10G003260.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G003260.1.exon.1CmaCh10G003260.1.exon.1exon


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 31..94
score: 2.8E-13coord: 105..167
score: 3.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 142..170
score: 2.6E-5coord: 32..60
score: 1.1E-4coord: 68..96
score: 8.3E-4coord: 106..134
score: 9.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 64..99
score: 10.831coord: 102..137
score: 13.509coord: 28..63
score: 15.071coord: 138..173
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..180
score: 2.3
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 30..174
score: 6.13
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
score: -coord: 115..127
score: -coord: 151..163
score: -coord: 41..53
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 27..172
score: 5.6
NoneNo IPR availablePANTHERPTHR10891:SF573CALCIUM-BINDING PROTEIN CML24-RELATEDcoord: 27..172
score: 5.6