Carg15175 (gene) Silver-seed gourd

Overview
NameCarg15175
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCalcium-binding protein
LocationCucurbita_argyrosperma_scaffold_073 : 181385 .. 181945 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCTGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCTGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCTGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTTGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCTGCCTTCTGCAAGGAACCGATGGCATCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

Protein sequence

MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAAAGP
Homology
[Close]
BLAST of Carg15175 vs. NCBI nr
Match: XP_022921374.1 (probable calcium-binding protein CML27 [Cucurbita moschata])

HSP 1 Score: 315.8 bits (808), Expect = 9.5e-83
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL
Sbjct: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180
           LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180

Query: 181 TAAAGP 187
           TAAAGP
Sbjct: 181 TAAAGP 186

[Close]
BLAST of Carg15175 vs. NCBI nr
Match: XP_022988487.1 (probable calcium-binding protein CML27 [Cucurbita maxima])

HSP 1 Score: 307.8 bits (787), Expect = 2.6e-80
Identity = 181/186 (97.31%), Postives = 181/186 (97.31%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATDSNPI SSNSDADQI KPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL
Sbjct: 1   MATDSNPIPSSNSDADQITKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GSAIPYXXXXXXXXXXXXXXXXXXXXXX AAFCKEPMASDEAGLSELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180
           LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSD NKSKAPNQN 
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDANKSKAPNQNA 180

Query: 181 TAAAGP 187
           TAAAGP
Sbjct: 181 TAAAGP 186

[Close]
BLAST of Carg15175 vs. NCBI nr
Match: XP_023515723.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.2 bits (744), Expect = 2.5e-75
Identity = 173/175 (98.86%), Postives = 173/175 (98.86%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL
Sbjct: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60

Query: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GSAIPYXXXXXXXXXXXXXXXXXXXXXX AAFCKEPMASDEAGLSELRDAFDLYDQDHNG
Sbjct: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXFAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKA 176
           LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMS TNKSKA
Sbjct: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSATNKSKA 175

[Close]
BLAST of Carg15175 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata])

HSP 1 Score: 252.3 bits (643), Expect = 1.3e-63
Identity = 129/186 (69.35%), Postives = 145/186 (77.96%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATDSN IT+SN+D +Q+ K KPS LL +N ELRKVFDRFD+NGDG+IS++ELDSVLKSL
Sbjct: 1   MATDSNQITASNADTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS++PY                       A FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180
           LIS SELHLVL RLGI  S EDCQRMIH+VDSDGDGNVNFEEFRKMMS+ + S+APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAGP 187
           TAA  P
Sbjct: 181 TAATAP 186

[Close]
BLAST of Carg15175 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.5 bits (641), Expect = 2.2e-63
Identity = 129/186 (69.35%), Postives = 144/186 (77.42%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATDSN I +SNSD +Q+ K KPS LL +N ELRKVFDRFD+NGDG+IS++ELDSVLKSL
Sbjct: 1   MATDSNQIAASNSDTEQVLKSKPSFLLQDNAELRKVFDRFDANGDGKISLSELDSVLKSL 60

Query: 61  GSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNG 120
           GS++PY                       A FCKEPMAS+EAG SELRDAFDLYDQDHNG
Sbjct: 61  GSSVPYDELRAVMEDLDTDKDGFINIDEFAVFCKEPMASNEAGASELRDAFDLYDQDHNG 120

Query: 121 LISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNG 180
           LIS SELHLVL RLGI  S EDCQRMIH+VDSDGDGNVNFEEFRKMMS+ + S+APNQNG
Sbjct: 121 LISQSELHLVLNRLGITCSKEDCQRMIHSVDSDGDGNVNFEEFRKMMSNNSNSEAPNQNG 180

Query: 181 TAAAGP 187
           TAA  P
Sbjct: 181 TAATAP 186

[Close]
BLAST of Carg15175 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 142.5 bits (358), Expect = 2.6e-34
Identity = 81/168 (48.21%), Postives = 104/168 (61.90%), Query Frame = 0 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXX 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++            
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  XXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
                         +  C+       +  +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of Carg15175 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 119.0 bits (297), Expect = 3.1e-27
Identity = 91/148 (61.49%), Postives = 107/148 (72.30%), Query Frame = 0 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G     XXXXXXXXXXXXXXXXXXXXXXX  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMG-----XXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
                         E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  XXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of Carg15175 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 95.9 bits (237), Expect = 2.8e-20
Identity = 56/140 (40.00%), Postives = 74/140 (52.86%), Query Frame = 0 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXX 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P                       
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  XAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
                 + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of Carg15175 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 88.2 bits (217), Expect = 5.8e-18
Identity = 49/138 (35.51%), Postives = 75/138 (54.35%), Query Frame = 0 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAA 91
           EL +VF  FD NGDG+I+  EL    KS+G  +P                         +
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY--SIEDCQRMIHT 151
             +E M  ++    ++R+AF ++DQ+ +G I+  EL  VL  +G+    ++EDC++MI  
Sbjct: 65  LYQE-MVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISK 124

Query: 152 VDSDGDGNVNFEEFRKMM 168
           VD DGDG VNF+EF++MM
Sbjct: 125 VDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Carg15175 vs. TAIR10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 84.7 bits (208), Expect = 6.4e-17
Identity = 74/163 (45.40%), Postives = 97/163 (59.51%), Query Frame = 0 

Query: 10  SSNSDADQIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXX 69
           S +  +  ++ P P G    +  E+R+VF RFD + DG+IS TE   VL++LG       
Sbjct: 27  SRDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQE---RA 86

Query: 70  XXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELH 129
              XXXXXXXXXXXXXXXXX   F      S     S++R++F  +D + +G IS  E+ 
Sbjct: 87  IEDXXXXXXXXXXXXXXXXXFREFIDAYKRSGGIRSSDIRNSFWTFDLNGDGKISAEEVM 146

Query: 130 LVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTN 172
            VL +LG   S+EDC RM+  VD+DGDG VN EEF KMMS  N
Sbjct: 147 SVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMMSSNN 186

[Close]
BLAST of Carg15175 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.7e-33
Identity = 81/168 (48.21%), Postives = 104/168 (61.90%), Query Frame = 0 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXX 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++            
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  XXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
                         +  C+       +  +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of Carg15175 vs. Swiss-Prot
Match: sp|Q9M7R0|ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 79/155 (50.97%), Postives = 95/155 (61.29%), Query Frame = 0 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXX 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS                   
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  XXXXXXXXAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
                   AAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPS-SGGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKS 174
           +  DC  MI +VDSDGDG V+FEEF+KMM  TNKS
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMM--TNKS 161

[Close]
BLAST of Carg15175 vs. Swiss-Prot
Match: sp|Q9C9U8|CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.6e-26
Identity = 91/148 (61.49%), Postives = 107/148 (72.30%), Query Frame = 0 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G     XXXXXXXXXXXXXXXXXXXXXXX  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMG-----XXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
                         E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  XXXXXXXXXXXXXVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of Carg15175 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.7e-22
Identity = 63/147 (42.86%), Postives = 82/147 (55.78%), Query Frame = 0 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +                         AA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of Carg15175 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.0e-19
Identity = 56/140 (40.00%), Postives = 74/140 (52.86%), Query Frame = 0 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXX 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P                       
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  XAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
                 + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of Carg15175 vs. TrEMBL
Match: tr|A0A1S3BX51|A0A1S3BX51_CUCME (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.4e-34
Identity = 117/188 (62.23%), Postives = 131/188 (69.68%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKS- 60
           MATD N I +S SDADQIAK KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL S 
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  -LGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
            L SAIP XXXXXXXXXXXXXXXXXXXXXXX                             
Sbjct: 61  TLKSAIPLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
                    HLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 XXXXXXXXXHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of Carg15175 vs. TrEMBL
Match: tr|A0A0A0LPP8|A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 9.2e-34
Identity = 116/188 (61.70%), Postives = 131/188 (69.68%), Query Frame = 0 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKS- 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL S 
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  -LGSAIPYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
            L SAIP XXXXXXXXXXXXXXXXXXXXXXX                             
Sbjct: 61  TLKSAIPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
                    HLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 XXXXXXXXXHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of Carg15175 vs. TrEMBL
Match: tr|A0A2I4DSW2|A0A2I4DSW2_9ROSI (probable calcium-binding protein CML27 OS=Juglans regia OX=51240 GN=LOC108983146 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.7e-33
Identity = 88/175 (50.29%), Postives = 110/175 (62.86%), Query Frame = 0 

Query: 5   SNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAI 64
           SNPI SS        + KPS  L   +E+ KVF+RFD+NGDG+IS++EL +VL SLG++ 
Sbjct: 7   SNPIESS--------QKKPSVYLENMDEVEKVFNRFDTNGDGKISVSELGAVLSSLGTSF 66

Query: 65  -PYXXXXXXXXXXXXXXXXXXXXXXXAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLIS 124
                                     AAFC+    S++ G SEL+DAF LYDQD NGLIS
Sbjct: 67  TDEGELRRIMAELDSDNDGFISLPEFAAFCRS--GSEDGGSSELQDAFRLYDQDQNGLIS 126

Query: 125 HSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 179
            +ELHLVL RLG+  SIE+C RMI +VDSDGDGNV+F+EF KMMS+ NKS    Q
Sbjct: 127 STELHLVLNRLGMKCSIEECHRMIRSVDSDGDGNVSFKEFEKMMSNANKSNGSAQ 171

[Close]
BLAST of Carg15175 vs. TrEMBL
Match: tr|A0A1J7G171|A0A1J7G171_LUPAN (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13804 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.0e-32
Identity = 76/138 (55.07%), Postives = 96/138 (69.57%), Query Frame = 0 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXXA 90
           EEL++VF+RFD+NGDG+IS+TELD+ L+SLGS++                         A
Sbjct: 26  EELKRVFNRFDANGDGKISVTELDTALRSLGSSVSTSEVQCIMEDLDTDHDGFINLSEFA 85

Query: 91  AFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTV 150
           AFC+      +  +SELRDAFDLYDQD NGLIS +ELHLVL RLG+  S+E+C  MI +V
Sbjct: 86  AFCR-----SDGDVSELRDAFDLYDQDKNGLISATELHLVLNRLGVKCSVEECHSMIKSV 145

Query: 151 DSDGDGNVNFEEFRKMMS 169
           DSDGDGNV+FEEF+KMMS
Sbjct: 146 DSDGDGNVSFEEFKKMMS 158

[Close]
BLAST of Carg15175 vs. TrEMBL
Match: tr|A0A059D1H4|A0A059D1H4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_B00943 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.7e-32
Identity = 103/147 (70.07%), Postives = 119/147 (80.95%), Query Frame = 0 

Query: 30  NEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYXXXXXXXXXXXXXXXXXXXXXXX 89
           NEELRKVFD+FD+NGDG+IS +EL  VL ++GSA+P XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 31  NEELRKVFDQFDANGDGKISASELSQVLAAMGSAVPPXXXXXXXXXXXXXXXXXXXXXXX 90

Query: 90  AAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 149
           A  C+   + D    ++LRDAFDLYDQD NGLIS +ELHLVL RL +N S+EDC RMI +
Sbjct: 91  AGLCRSGPSPD----ADLRDAFDLYDQDRNGLISAAELHLVLNRLSMNCSVEDCARMIKS 150

Query: 150 VDSDGDGNVNFEEFRKMMSDTNKSKAP 177
           VD DGDGNVNFEEFRKMM+    SK P
Sbjct: 151 VDGDGDGNVNFEEFRKMMA----SKGP 169

The following BLAST results are available for this feature:
BLAST of Carg15175 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022921374.19.5e-83100.00probable calcium-binding protein CML27 [Cucurbita moschata][more]
XP_022988487.12.6e-8097.31probable calcium-binding protein CML27 [Cucurbita maxima][more]
XP_023515723.12.5e-7598.86probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo][more]
XP_022933917.11.3e-6369.35probable calcium-binding protein CML27 [Cucurbita moschata][more]
XP_023529417.12.2e-6369.35probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo][more]
BLAST of Carg15175 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.12.6e-3448.21Calcium-binding EF-hand family protein[more]
AT1G73630.13.1e-2761.49EF hand calcium-binding protein family[more]
AT1G24620.12.8e-2040.00EF hand calcium-binding protein family[more]
AT4G12860.15.8e-1835.51EF hand calcium-binding protein family[more]
AT2G15680.16.4e-1745.40Calcium-binding EF-hand family protein[more]
BLAST of Carg15175 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9LE22|CML27_ARATH4.7e-3348.21Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
sp|Q9M7R0|ALL8_OLEEU1.1e-2950.97Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1[more]
sp|Q9C9U8|CML26_ARATH5.6e-2661.49Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ... [more]
sp|Q0DJV6|CML18_ORYSJ3.7e-2242.86Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q9FYK2|CML25_ARATH5.0e-1940.00Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
BLAST of Carg15175 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BX51|A0A1S3BX51_CUCME5.4e-3462.23probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103494178 P... [more]
tr|A0A0A0LPP8|A0A0A0LPP8_CUCSA9.2e-3461.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1[more]
tr|A0A2I4DSW2|A0A2I4DSW2_9ROSI2.7e-3350.29probable calcium-binding protein CML27 OS=Juglans regia OX=51240 GN=LOC108983146... [more]
tr|A0A1J7G171|A0A1J7G171_LUPAN1.0e-3255.07Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13804 PE=4 S... [more]
tr|A0A059D1H4|A0A059D1H4_EUCGR1.7e-3270.07Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_B00943 PE=4 SV... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15175-RACarg15175-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 106..134
e-value: 9.7E-5
score: 31.8
coord: 68..96
e-value: 0.0017
score: 27.6
coord: 142..170
e-value: 8.9E-6
score: 35.2
coord: 32..60
e-value: 1.1E-4
score: 31.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 105..167
e-value: 5.1E-13
score: 49.2
coord: 31..94
e-value: 3.2E-13
score: 49.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 28..63
score: 15.071
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 64..99
score: 10.608
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 102..137
score: 13.509
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 138..173
score: 14.207
IPR002048EF-hand domainCDDcd00051EFhcoord: 106..168
e-value: 2.59428E-19
score: 77.2029
IPR002048EF-hand domainCDDcd00051EFhcoord: 32..92
e-value: 3.41679E-15
score: 66.0321
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 100..183
e-value: 1.2E-23
score: 85.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 9..99
e-value: 4.2E-20
score: 74.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 20..178
NoneNo IPR availablePANTHERPTHR10891:SF737SUBFAMILY NOT NAMEDcoord: 20..178
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 151..163
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 41..53
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 115..127
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 30..179

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg15175Cla018929Watermelon (97103) v1carwmB0789
Carg15175Csa1G025020Cucumber (Chinese Long) v2carcuB0801
Carg15175MELO3C017353Melon (DHL92) v3.5.1carmeB0785
Carg15175ClCG06G014370Watermelon (Charleston Gray)carwcgB0764
Carg15175CSPI01G04290Wild cucumber (PI 183967)carcpiB0821
Carg15175Cucsa.111510Cucumber (Gy14) v1carcgyB0897
Carg15175CmaCh11G003070Cucurbita maxima (Rimu)carcmaB1092
Carg15175CmaCh10G003260Cucurbita maxima (Rimu)carcmaB1091
Carg15175CmoCh11G003140Cucurbita moschata (Rifu)carcmoB1064
Carg15175CmoCh10G003510Cucurbita moschata (Rifu)carcmoB1063
Carg15175Lsi06G012900Bottle gourd (USVL1VR-Ls)carlsiB726
Carg15175Cp4.1LG18g06750Cucurbita pepo (Zucchini)carcpeB1103
Carg15175Cp4.1LG04g13590Cucurbita pepo (Zucchini)carcpeB1106
Carg15175CsGy1G004180Cucumber (Gy14) v2carcgybB0778
Carg15175MELO3C017353.2Melon (DHL92) v3.6.1carmedB0797
Carg15175CsaV3_1G004290Cucumber (Chinese Long) v3carcucB0824
Carg15175Cla97C06G123830Watermelon (97103) v2carwmbB0827
Carg15175Bhi02G000096Wax gourdcarwgoB0924
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg15175Carg09105Silver-seed gourdcarcarB537
The following block(s) are covering this gene:
GeneOrganismBlock
Carg15175Silver-seed gourdcarcarB418
Carg15175Silver-seed gourdcarcarB616
Carg15175Cucumber (Gy14) v1carcgyB0898
Carg15175Cucurbita maxima (Rimu)carcmaB1094
Carg15175Cucurbita maxima (Rimu)carcmaB1095
Carg15175Cucurbita moschata (Rifu)carcmoB1065
Carg15175Cucurbita pepo (Zucchini)carcpeB1104
Carg15175Cucumber (Chinese Long) v2carcuB0802
Carg15175Cucumber (Chinese Long) v3carcucB0825
Carg15175Bottle gourd (USVL1VR-Ls)carlsiB724
Carg15175Melon (DHL92) v3.5.1carmeB0783
Carg15175Melon (DHL92) v3.6.1carmedB0795
Carg15175Watermelon (Charleston Gray)carwcgB0762
Carg15175Watermelon (97103) v1carwmB0786
Carg15175Watermelon (97103) v2carwmbB0825
Carg15175Wax gourdcarwgoB0922