CmoCh10G003510.1 (mRNA) Cucurbita moschata (Rifu)

Overview
NameCmoCh10G003510.1
TypemRNA
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionCalcium-binding allergen Ole e 8
LocationCmo_Chr10 : 1594362 .. 1594922 (-)
Sequence length561
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

mRNA sequence

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG

Coding sequence (CDS)

ATGGCCACTGACTCGAATCCGATTACGTCCTCTAACTCCGACGCCGACCAGATCGCCAAGCCGAAGCCCTCCGGCCTCCTCCATGAAAACGAGGAACTCCGCAAGGTTTTCGACCGCTTCGATTCGAATGGCGACGGCAGGATCTCCATCACCGAGCTCGATTCCGTCCTCAAGTCCTTAGGCTCTGCCATCCCCTACGAGGAGCTACGTGGCGTCATGGAGGATCTCGACTACGATAAGGACGGTTTCATCAACATAGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAGGCTGGATTGTCGGAGCTTCGTGACGCCTTCGACCTCTACGATCAGGACCACAACGGCCTCATCTCTCACTCCGAGCTCCATCTCGTCCTCACTCGCCTCGGAATCAATTACTCCATTGAAGACTGTCAACGAATGATCCACACGGTCGATTCCGATGGCGACGGAAATGTCAATTTCGAAGAGTTTAGGAAGATGATGAGCGATACTAACAAATCCAAGGCGCCGAATCAGAATGGAACTGCAGCCGCGGGGCCCTAG
Homology
[Close]
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.9e-45
Identity = 94/168 (55.95%), Postives = 124/168 (73.81%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  V+E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match: ALL8_OLEEU (Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.4e-42
Identity = 93/155 (60.00%), Postives = 114/155 (73.55%), Query Frame = 1 

Query: 22  KPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKD 81
           KPS  L E  E++ VF+RFD+NGDG+IS  EL  VLK+LGS    EE+  +ME++D DKD
Sbjct: 10  KPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKD 69

Query: 82  GFINIDEFAAFCK---EPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINY 141
           GFIN+ EFAAF K   +P  S   G +EL++AF+LYDQDHNGLIS  ELH +LTRLG  Y
Sbjct: 70  GFINVQEFAAFVKAETDPYPSS-GGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 129

Query: 142 SIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKS 174
           +  DC  MI +VDSDGDG V+FEEF+KMM  TNKS
Sbjct: 130 AEHDCVEMIKSVDSDGDGYVSFEEFKKMM--TNKS 161

[Close]
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match: CML26_ARATH (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-40
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  V++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.2e-33
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.3e-32
Identity = 73/147 (49.66%), Postives = 101/147 (68.71%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL +VF R+D+NGDG+IS  EL SVL++LG+ +   E+R +M+++D D+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 92  FCKEPMAS-----------DEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSI 151
           F   P  +           + A  +ELR+AF +YD D NG IS  ELH VL +LG   S+
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 152 EDCQRMIHTVDSDGDGNVNFEEFRKMM 168
            DC RMI +VD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match: A0A0A0LPP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 9.3e-73
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match: Q93YA8_SESRO (Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.7e-47
Identity = 97/169 (57.40%), Postives = 128/169 (75.74%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  VM+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match: A0A0R4J3Q2_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.1e-46
Identity = 103/175 (58.86%), Postives = 132/175 (75.43%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VMEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

[Close]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match: V7CQX7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-44
Identity = 96/165 (58.18%), Postives = 127/165 (76.97%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L + +E+++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIATGNGDAAPNPDAGAKSSVYLQDMDEMKRVFSRFDTNGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VM+DLD D DGFIN+ EFAAFC+   A  + G +EL+DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQNVMDDLDTDHDGFINLSEFAAFCRAETA--DGGDTELQDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMM 168
           S +EL  VL RLG+  SI++C  MI +VDSDGDG+VNFEEF++MM
Sbjct: 123 SATELCQVLNRLGMRCSIDECHSMIKSVDSDGDGDVNFEEFKRMM 165

[Close]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match: A0A0R0K5I7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-44
Identity = 99/175 (56.57%), Postives = 129/175 (73.71%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  KPS  L + EEL++VF RFD+N DG+IS+TELD+VL+SLGS
Sbjct: 39  TNPIEAGNGDAAPNPNATTKPSVYLQDTEELKRVFSRFDANCDGKISVTELDNVLRSLGS 98

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P E+++ VM+DLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYD D NG I
Sbjct: 99  GVPPEDIQRVMDDLDTDHDGFINLSEFAAFCRSDTA--DGGDAELHDAFNLYDHDKNGHI 158

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 159 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 210

[Close]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 181.8 bits (460), Expect = 3.9e-46
Identity = 94/168 (55.95%), Postives = 124/168 (73.81%), Query Frame = 1 

Query: 17  QIAKPKPSGLLHEN-EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMED 76
           + AKP P+ +   N EEL+KVFD+FDSNGDG+IS+ EL  V K++G++    EL  V+E+
Sbjct: 7   ETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEE 66

Query: 77  LDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLG 136
           +D D+DG+IN+DEF+  C+   ++     +E+RDAFDLYDQD NGLIS SELH VL RLG
Sbjct: 67  VDTDRDGYINLDEFSTLCRSSSSA-----AEIRDAFDLYDQDKNGLISASELHQVLNRLG 126

Query: 137 INYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQNGTAA 184
           ++ S+EDC RMI  VD+DGDGNVNFEEF+KMM+    S   N NG+AA
Sbjct: 127 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT---SSSLLNSNGSAA 166

[Close]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 166.4 bits (420), Expect = 1.7e-41
Identity = 83/148 (56.08%), Postives = 109/148 (73.65%), Query Frame = 1 

Query: 32  ELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFAA 91
           EL+KVFD+FD+NGDG+IS++EL +V KS+G++   EEL  V++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 92  FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHTVD 151
            C+   ++      E+R+AFDLYDQ+ NGLIS SE+H VL RLG+  S+EDC RMI  VD
Sbjct: 80  ICRSSSSA-----VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 152 SDGDGNVNFEEFRKMMSDTNKSKAPNQN 180
           +DGDGNVNFEEF+KMMS     K    N
Sbjct: 140 TDGDGNVNFEEFQKMMSSPELVKGTVAN 162

[Close]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 144.4 bits (363), Expect = 6.8e-35
Identity = 72/138 (52.17%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           E+++KVF RFD N DG+ISI EL  V+ +L      EE + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 91  A-FCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIHT 150
           A F     +S+ + + +L++AFDLYD D NG IS +ELH V+  LG   SI+DCQRMI+ 
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 151 VDSDGDGNVNFEEFRKMM 168
           VDSDGDG V+FEEF+KMM
Sbjct: 134 VDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.1e-31
Identity = 66/142 (46.48%), Postives = 97/142 (68.31%), Query Frame = 1 

Query: 31  EELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDEFA 90
           ++++KVF RFD NGDG+IS+ EL  V+++L      EE   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 91  AFCKEPMA---SDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMI 150
           A  +  +    ++   +S+L++AF+LYD D NG IS  ELH V+  LG   S++DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 151 HTVDSDGDGNVNFEEFRKMMSD 170
             VD DGDG VNF+EF+KMMS+
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMSN 157

[Close]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 126.3 bits (316), Expect = 1.9e-29
Identity = 66/140 (47.14%), Postives = 89/140 (63.57%), Query Frame = 1 

Query: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDE 88
           E  EL  VF +FD NGDG+IS  EL +++ SLG  +P EEL   + ++D   DG+IN +E
Sbjct: 34  EIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEE 93

Query: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148
           F     + M  ++  L  L+DAF +YD D NG IS  ELH VL  LG   SI +C++MI 
Sbjct: 94  FVELNTKGMDQNDV-LENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 153

Query: 149 TVDSDGDGNVNFEEFRKMMS 169
            VD DGDG ++FEEF+ MM+
Sbjct: 154 GVDKDGDGTIDFEEFKIMMT 172

[Close]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match: gi|449439129|ref|XP_004137340.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus])

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-72
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD NPI +S S+ADQIAK  PS LL ++EELRKVF+RFD+NGDG+ISI+ELD+VL SL
Sbjct: 1   MATDPNPIPTSKSEADQIAKQNPSVLLQDDEELRKVFERFDANGDGKISISELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INIDEFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIDEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAQQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match: gi|659106947|ref|XP_008453475.1| (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 2.3e-72
Identity = 145/188 (77.13%), Postives = 164/188 (87.23%), Query Frame = 1 

Query: 1   MATDSNPITSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSL 60
           MATD N I +S SDADQIAK KPS LL +++ELRKVF+RFD+NGDG+ISITELD+VL SL
Sbjct: 1   MATDPNQIPTSQSDADQIAKQKPSVLLEDDDELRKVFERFDANGDGKISITELDAVLTSL 60

Query: 61  G--SAIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDH 120
              SAIP EELR VM+DLD DKDG+INI+EFAAFCK+PMASDEAG +ELRDAFDLYDQD 
Sbjct: 61  TLKSAIPLEELRSVMDDLDSDKDGYINIEEFAAFCKKPMASDEAGAAELRDAFDLYDQDR 120

Query: 121 NGLISHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPNQ 180
           NGLIS SELHLVL RLGI+ S EDCQ+MI++VDSDGDGNVNFEEFRKMM+D +KSKA  Q
Sbjct: 121 NGLISQSELHLVLNRLGISCSKEDCQKMINSVDSDGDGNVNFEEFRKMMTDNSKSKAAKQ 180

Query: 181 NGTAAAGP 187
           NGTAAA P
Sbjct: 181 NGTAAAAP 188

[Close]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match: gi|1009165393|ref|XP_015901010.1| (PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba])

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-48
Identity = 105/169 (62.13%), Postives = 133/169 (78.70%), Query Frame = 1 

Query: 9   TSSNSDADQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEE 68
           T+S S+++   KPK S  +   +E++KVF++FD+NGDG+ISITEL+ VL++LGS +P EE
Sbjct: 3   TTSPSESE---KPKSSVSIQNMDEVKKVFNQFDANGDGKISITELEKVLETLGSEVPQEE 62

Query: 69  LRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELH 128
           L  VM+DLD D+DGFI +DEFAAFC+    +++A  SELRDAFDLYD+DHNGLIS  ELH
Sbjct: 63  LHRVMDDLDTDRDGFIGLDEFAAFCRS--GTEDAADSELRDAFDLYDRDHNGLISSEELH 122

Query: 129 LVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           LVL RLG+N S EDC+RMI +VDSDGDGNVNF EF+KMM  TNK    N
Sbjct: 123 LVLNRLGMNCSPEDCRRMIRSVDSDGDGNVNFAEFQKMM--TNKGSNGN 164

[Close]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match: gi|14589311|emb|CAC43238.1| (calcium binding protein [Sesbania rostrata])

HSP 1 Score: 195.3 bits (495), Expect = 9.6e-47
Identity = 97/169 (57.40%), Postives = 128/169 (75.74%), Query Frame = 1 

Query: 5   SNPITSSNSDADQ--IAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NP    N+  +Q    K KPS  + + +EL++VF RFD+NGDG+IS+ ELD+VL++LGS
Sbjct: 3   TNPTAPENTVTEQNPATKTKPSVYMEDMDELKRVFSRFDANGDGKISVNELDNVLRALGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P +EL  VM+DLD D DGFIN+ EFAAFC+   A  + G SELR+AFDLYDQD NGLI
Sbjct: 63  TVPSDELERVMKDLDTDNDGFINLTEFAAFCRSDAA--DGGASELREAFDLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTN 172
           S +EL LVL RLG+  S+E+C  MI +VDSDGDGNVNF+EF++MM++ N
Sbjct: 123 SAAELCLVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFDEFKQMMTNNN 169

[Close]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match: gi|356524678|ref|XP_003530955.1| (PREDICTED: calcium-binding allergen Ole e 8 [Glycine max])

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-46
Identity = 103/175 (58.86%), Postives = 132/175 (75.43%), Query Frame = 1 

Query: 5   SNPITSSNSDA--DQIAKPKPSGLLHENEELRKVFDRFDSNGDGRISITELDSVLKSLGS 64
           +NPI + N DA  +  A  K S  L ++EEL++VF RFD+NGDG+IS++ELD+VL+SLGS
Sbjct: 3   TNPIEAGNGDAAPNPNATTKSSVYLQDSEELKRVFSRFDANGDGKISVSELDNVLRSLGS 62

Query: 65  AIPYEELRGVMEDLDYDKDGFINIDEFAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLI 124
            +P EEL+ VMEDLD D DGFIN+ EFAAFC+   A  + G +EL DAF+LYDQD NGLI
Sbjct: 63  GVPPEELQRVMEDLDTDHDGFINLSEFAAFCRSDTA--DGGDTELHDAFNLYDQDKNGLI 122

Query: 125 SHSELHLVLTRLGINYSIEDCQRMIHTVDSDGDGNVNFEEFRKMMSDTNKSKAPN 178
           S +EL  VL RLG+  S+E+C  MI +VDSDGDGNVNF EF++MMS+ N+  A N
Sbjct: 123 SATELCQVLNRLGMKCSVEECHNMIKSVDSDGDGNVNFPEFKRMMSN-NRENASN 174

The following BLAST results are available for this feature:
BLAST of CmoCh10G003510.1 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML27_ARATH6.9e-4555.95Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=... [more]
ALL8_OLEEU5.4e-4260.00Calcium-binding allergen Ole e 8 OS=Olea europaea PE=1 SV=1[more]
CML26_ARATH3.0e-4056.08Probable calcium-binding protein CML26 OS=Arabidopsis thaliana GN=CML26 PE=1 SV=... [more]
CML23_ARATH1.2e-3352.17Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML18_ORYSJ1.3e-3249.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of CmoCh10G003510.1 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPP8_CUCSA9.3e-7377.13Uncharacterized protein OS=Cucumis sativus GN=Csa_1G025020 PE=4 SV=1[more]
Q93YA8_SESRO6.7e-4757.40Calcium binding protein OS=Sesbania rostrata GN=cbl1 PE=2 SV=1[more]
A0A0R4J3Q2_SOYBN1.1e-4658.86Uncharacterized protein OS=Glycine max GN=GLYMA_08G053500 PE=4 SV=1[more]
V7CQX7_PHAVU1.1e-4458.18Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G329300g PE=4 SV=1[more]
A0A0R0K5I7_SOYBN1.4e-4456.57Uncharacterized protein OS=Glycine max GN=GLYMA_05G245600 PE=4 SV=1[more]
BLAST of CmoCh10G003510.1 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18210.13.9e-4655.95 Calcium-binding EF-hand family protein[more]
AT1G73630.11.7e-4156.08 EF hand calcium-binding protein family[more]
AT1G66400.16.8e-3552.17 calmodulin like 23[more]
AT5G37770.12.1e-3146.48 EF hand calcium-binding protein family[more]
AT1G24620.11.9e-2947.14 EF hand calcium-binding protein family[more]
BLAST of CmoCh10G003510.1 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449439129|ref|XP_004137340.1|1.3e-7277.13PREDICTED: probable calcium-binding protein CML27 [Cucumis sativus][more]
gi|659106947|ref|XP_008453475.1|2.3e-7277.13PREDICTED: probable calcium-binding protein CML27 [Cucumis melo][more]
gi|1009165393|ref|XP_015901010.1|1.0e-4862.13PREDICTED: probable calcium-binding protein CML27 [Ziziphus jujuba][more]
gi|14589311|emb|CAC43238.1|9.6e-4757.40calcium binding protein [Sesbania rostrata][more]
gi|356524678|ref|XP_003530955.1|1.6e-4658.86PREDICTED: calcium-binding allergen Ole e 8 [Glycine max][more]
Annotated Terms
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmoCh10G003510CmoCh10G003510gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmoCh10G003510.1CmoCh10G003510.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G003510.1.CDS.1CmoCh10G003510.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmoCh10G003510.1.exon.1CmoCh10G003510.1.exon.1exon


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 31..94
score: 3.1E-13coord: 105..167
score: 3.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 32..60
score: 1.1E-4coord: 142..170
score: 8.9E-6coord: 68..96
score: 0.0017coord: 106..134
score: 9.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 64..99
score: 10.608coord: 102..137
score: 13.509coord: 28..63
score: 15.071coord: 138..173
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..181
score: 1.7
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 30..179
score: 3.66
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 77..89
score: -coord: 41..53
score: -coord: 151..163
score: -coord: 115..127
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 27..172
score: 5.8
NoneNo IPR availablePANTHERPTHR10891:SF573CALCIUM-BINDING PROTEIN CML24-RELATEDcoord: 27..172
score: 5.8