CmoCh04G000710 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh04G000710
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium binding family protein
Location	Cmo_Chr04 : 383092 .. 383652 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTTTTCGACGCGGATGGCGATGGATTCATTAATCTACAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTCATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGTGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGTTGGGCTCTCCGTCGCAGGCGGTTGA

mRNA sequence

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTTTTCGACGCGGATGGCGATGGATTCATTAATCTACAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTCATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGTGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGTTGGGCTCTCCGTCGCAGGCGGTTGA

Coding sequence (CDS)

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTTTTCGACGCGGATGGCGATGGATTCATTAATCTACAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTCATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGTGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGTTGGGCTCTCCGTCGCAGGCGGTTGA

Homology

[Close]

BLAST of CmoCh04G000710 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 7.6e-52
Identity = 99/147 (67.35%), Postives = 122/147 (82.99%), Query Frame = 1


Query: 34  SRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDG 93
           +R   +   ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL K I   D  GDG
Sbjct: 28  ARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDG 87

Query: 94  FINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADC 153
           +IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+RSLGD+CS+A+C
Sbjct: 88  YINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAEC 147

Query: 154 RQMISGVDRNGDGLISFDEFKVMMSVG 181
           R+MI GVD++GDG I F+EFK+MM++G
Sbjct: 148 RKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmoCh04G000710 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.9e-40
Identity = 85/143 (59.44%), Postives = 108/143 (75.52%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E+E VF+KFD NGDG+IS +ELG++  SLGH AT++ELA+M+   DADGDGFI+L EF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 103 LN-TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVD 162
           LN T   D   + E+LR AF V+D DGNG+ISA EL +V+  LG+  +V  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 163 RNGDGLISFDEFKVMMSVGLSVA 185
           +NGDGLISF+EFKVMM+ G S A
Sbjct: 160 QNGDGLISFEEFKVMMAGGGSFA 182

[Close]

BLAST of CmoCh04G000710 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 73/139 (52.52%), Postives = 102/139 (73.38%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+A+M++  D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 103 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVD 162
           ++     D     E+LR AF V+D DGNG I+  EL +V+R +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 163 RNGDGLISFDEFKVMMSVG 181
           RNGDGLI+F+EFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]

BLAST of CmoCh04G000710 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 7.9e-33
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 GVDRNGDGLISFDEFKVMMSVG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmoCh04G000710 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.3e-32
Identity = 74/151 (49.01%), Postives = 97/151 (64.24%), Query Frame = 1


Query: 42  AELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFV 101
           AELE+VF+++D NGDGKIS+ EL S++ +LG      E+ +M++  D+D DGF++L EF 
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 102 ELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVMRSLGDDCS 161
             +               D E   E  LREAF +YD D NG ISA ELH+V+R LGD CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 162 VADCRQMISGVDRNGDGLISFDEFKVMMSVG 181
           VADC +MI  VD +GDG ++FDEFK MM  G
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]

BLAST of CmoCh04G000710 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.2e-75
Identity = 155/185 (83.78%), Postives = 164/185 (88.65%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]

BLAST of CmoCh04G000710 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-56
Identity = 121/180 (67.22%), Postives = 145/180 (80.56%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   SR   ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGSR---TQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+LENL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVMM  G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]

BLAST of CmoCh04G000710 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.0e-55
Identity = 121/191 (63.35%), Postives = 146/191 (76.44%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFA-SVTSSIVSSPSTNGPRSSSRDSHSRNA----------ELEEVFK 60
           M F  +F  KKK  + S  S++  SP+     SS   S  R            ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEID 120
           KFDVNGDGKISS+ELGSIMGSLGHK +E+EL KMI+ FDADGDG IN +EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLIS 180
            +E+LENL++AFSVYDIDGNGSISAEELH+V++SLGD+CS+A+CR+MISGVD +GDG+I 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]

BLAST of CmoCh04G000710 vs. TrEMBL
Match: A0A151S400_CAJCA (Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.3e-55
Identity = 118/187 (63.10%), Postives = 144/187 (77.01%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNG-PRSSSRDSHSRNAE-LEEVFKKFDVNGDGK 60
           M F+ LFC K+K   + +SS  S+ ST+  P +       R AE LE VFKKFDVNGDGK
Sbjct: 1   MGFRSLFCRKRKMLKTTSSSSASTSSTSALPTAIPGSGRGRVAEELEYVFKKFDVNGDGK 60

Query: 61  ISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLR 120
           ISS+ELGS+M SLG  ATEEEL+KMI   DADGDG I+L+EF+ELNTK +DP+E+LENLR
Sbjct: 61  ISSSELGSMMRSLGQAATEEELSKMIGEVDADGDGHISLEEFIELNTKGVDPDEVLENLR 120

Query: 121 EAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMS 180
           +AFS++DIDGNGSI+ EELH VM SLGD CSV DCR+MI+GVD +GDG I FDEFK+MM+
Sbjct: 121 DAFSIFDIDGNGSITVEELHMVMASLGDQCSVDDCRKMIAGVDADGDGTIDFDEFKIMMT 180

Query: 181 VGLSVAG 186
              S +G
Sbjct: 181 ASASASG 187

[Close]

BLAST of CmoCh04G000710 vs. TrEMBL
Match: A0A067KPP2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.9e-55
Identity = 120/184 (65.22%), Postives = 140/184 (76.09%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSR----NAELEEVFKKFDVNGD 60
           M FK LF  KK   +S +S +  + S   P S S     R      EL+ VF KFD NGD
Sbjct: 1   MGFKALFNRKKSKKSSDSSVVSPAGSPPDPLSRSGSQSLRLKHQTDELQHVFNKFDANGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLEN 120
           GKISSAELGSIM SLGHKAT+EEL KMI  FDADGDGFI+ QEFVELNT+ +D  E +EN
Sbjct: 61  GKISSAELGSIMASLGHKATDEELRKMIMEFDADGDGFIDFQEFVELNTQGVDTNEAMEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVM 180
           LR+AFSVYDIDGNGSISAEELH+VM SLG+ CS+A+CR+MISGVD +GDG+I F+EFKVM
Sbjct: 121 LRDAFSVYDIDGNGSISAEELHRVMGSLGESCSIAECRRMISGVDGDGDGMIDFEEFKVM 180

[Close]

BLAST of CmoCh04G000710 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 204.9 bits (520), Expect = 4.3e-53
Identity = 99/147 (67.35%), Postives = 122/147 (82.99%), Query Frame = 1


Query: 34  SRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDG 93
           +R   +   ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL K I   D  GDG
Sbjct: 28  ARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDG 87

Query: 94  FINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADC 153
           +IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+RSLGD+CS+A+C
Sbjct: 88  YINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAEC 147

Query: 154 RQMISGVDRNGDGLISFDEFKVMMSVG 181
           R+MI GVD++GDG I F+EFK+MM++G
Sbjct: 148 RKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmoCh04G000710 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 141.7 bits (356), Expect = 4.4e-34
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 GVDRNGDGLISFDEFKVMMSVG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmoCh04G000710 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-32
Identity = 68/151 (45.03%), Postives = 104/151 (68.87%), Query Frame = 1


Query: 31  RSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDAD 90
           ++ SR+      ++++VF++FD N DGKIS  EL  ++G+L   A++EE   M++ FD D
Sbjct: 3   KNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLD 62

Query: 91  GDGFINLQEFVELN--TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDC 150
           G+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH VM++LG+ C
Sbjct: 63  GNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKC 122

Query: 151 SVADCRQMISGVDRNGDGLISFDEFKVMMSV 180
           S+ DC++MI+ VD +GDG + F+EFK MM +
Sbjct: 123 SIQDCQRMINKVDSDGDGCVDFEEFKKMMMI 153

[Close]

BLAST of CmoCh04G000710 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.1 bits (331), Expect = 3.5e-31
Identity = 71/153 (46.41%), Postives = 100/153 (65.36%), Query Frame = 1


Query: 26  STNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIE 85
           +TN   ++   + S + EL++VF KFD NGDGKIS +ELG++  S+G   TEEEL ++++
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 86  VFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLG 145
             D D DGFIN +EF  +        E+    REAF +YD + NG IS+ E+HKV+  LG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 146 DDCSVADCRQMISGVDRNGDGLISFDEFKVMMS 179
             CSV DC +MI  VD +GDG ++F+EF+ MMS
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of CmoCh04G000710 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 126.7 bits (317), Expect = 1.5e-29
Identity = 71/156 (45.51%), Postives = 98/156 (62.82%), Query Frame = 1


Query: 23  SSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAK 82
           ++P T  P  ++ D  +   EL++VF +FD NGDGKIS  ELG +  ++G   TE EL +
Sbjct: 4   ANPETAKPTPATVDM-ANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 63

Query: 83  MIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMR 142
           ++E  D D DG+INL EF  L        E+    R+AF +YD D NG ISA ELH+V+ 
Sbjct: 64  VLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLN 123

Query: 143 SLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMS 179
            LG  CSV DC +MI  VD +GDG ++F+EF+ MM+
Sbjct: 124 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMT 154

[Close]

BLAST of CmoCh04G000710 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 294.3 bits (752), Expect = 1.5e-76
Identity = 158/186 (84.95%), Postives = 165/186 (88.71%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS   S S+ AELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMIE FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180
           FSVYDIDGNGSISAEELHKV+RSLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 185

[Close]

BLAST of CmoCh04G000710 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 290.8 bits (743), Expect = 1.7e-75
Identity = 155/185 (83.78%), Postives = 164/185 (88.65%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]

BLAST of CmoCh04G000710 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-56
Identity = 121/180 (67.22%), Postives = 145/180 (80.56%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   SR   ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGSR---TQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+LENL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSVG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVMM  G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]

BLAST of CmoCh04G000710 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-56
Identity = 121/184 (65.76%), Postives = 144/184 (78.26%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSR----DSHSRNAELEEVFKKFDVNGD 60
           M FK LF  KKK          S PST+ P  S+      S ++ AELE+VF KFDVNGD
Sbjct: 1   MGFKSLFSRKKK----------SHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLEN 120
           GKI S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D  E+LEN
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVM 180
           L++AFSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVM 174

[Close]

BLAST of CmoCh04G000710 vs. NCBI nr
Match: gi|743822788|ref|XP_011021780.1| (PREDICTED: probable calcium-binding protein CML25 [Populus euphratica])

HSP 1 Score: 226.5 bits (576), Expect = 3.9e-56
Identity = 122/185 (65.95%), Postives = 146/185 (78.92%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPS-----TNGPRSSSRDSHSRNAELEEVFKKFDVNG 60
           M FK LF  KKK  +  + S V SPS     TNG +S S     +  ELE+VFKKFDVNG
Sbjct: 1   MGFKALFNRKKK--SKKSDSSVDSPSAIVTSTNGSKSPSIRLRPQVQELEQVFKKFDVNG 60

Query: 61  DGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLE 120
           DGKISSAELGSIM SLGH+ATE+EL  MI  FDADGDGFI+LQEFV LNT+ +D  E++E
Sbjct: 61  DGKISSAELGSIMASLGHEATEDELKTMITEFDADGDGFIDLQEFVALNTQGVDTNEVME 120

Query: 121 NLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKV 180
           NL++AFSVYD+DGNGSISAEELHKVM SLG+ CS+A+CR++ISGVD +GDG+I F+EFKV
Sbjct: 121 NLKDAFSVYDVDGNGSISAEELHKVMASLGEPCSMAECRKIISGVDSDGDGMIDFEEFKV 180

The following BLAST results are available for this feature:

BLAST of CmoCh04G000710 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML25_ARATH	7.6e-52	67.35	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML10_ORYSJ	3.9e-40	59.44	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]
CML15_ORYSJ	1.7e-35	52.52	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ...	[more]
CML24_ARATH	7.9e-33	51.41	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML18_ORYSJ	2.3e-32	49.01	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ...	[more]

BLAST of CmoCh04G000710 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX11_CUCSA	1.2e-75	83.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1	[more]
A0A0A0KST2_CUCSA	1.2e-56	67.22	Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1	[more]
A0A061E9N9_THECC	1.0e-55	63.35	Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1	[more]
A0A151S400_CAJCA	2.3e-55	63.10	Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1	[more]
A0A067KPP2_JATCU	3.9e-55	65.22	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1	[more]

BLAST of CmoCh04G000710 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	4.3e-53	67.35	EF hand calcium-binding protein family	[more]
AT5G37770.1	4.4e-34	51.41	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.1e-32	45.03	calmodulin like 23	[more]
AT1G73630.1	3.5e-31	46.41	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.5e-29	45.51	Calcium-binding EF-hand family protein	[more]

BLAST of CmoCh04G000710 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659129528\|ref\|XP_008464718.1\|	1.5e-76	84.95	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]
gi\|449449242\|ref\|XP_004142374.1\|	1.7e-75	83.78	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus]	[more]
gi\|778703916\|ref\|XP_011655449.1\|	1.7e-56	67.22	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus]	[more]
gi\|659072787\|ref\|XP_008467096.1\|	3.0e-56	65.76	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]
gi\|743822788\|ref\|XP_011021780.1\|	3.9e-56	65.95	PREDICTED: probable calcium-binding protein CML25 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0048767	root hair elongation
biological_process	GO:0048868	pollen tube development
biological_process	GO:0009409	response to cold
biological_process	GO:0008150	biological_process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G000710.1	CmoCh04G000710.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 43..103 score: 7.8E-13coord: 115..177 score: 1.5
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 152..180 score: 1.8E-4coord: 79..107 score: 7.2E-5coord: 43..71 score: 4.7E-7coord: 116..144 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 75..110 score: 13.342coord: 112..147 score: 15.741coord: 148..183 score: 12.365coord: 39..74 score: 15
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 37..177 score: 5.9
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 40..178 score: 1.92
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 52..64 score: -coord: 88..100 score: -coord: 161..173 score: -coord: 125..137 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 43..180 score: 8.8
None	No IPR available	PANTHER	PTHR10891:SF605	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 43..180 score: 8.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmoCh04G000710	Cucsa.029820	Cucumber (Gy14) v1	cgycmoB0002
CmoCh04G000710	CmaCh04G000750	Cucurbita maxima (Rimu)	cmacmoB728
CmoCh04G000710	CmaCh04G010210	Cucurbita maxima (Rimu)	cmacmoB731
CmoCh04G000710	CmaCh04G021050	Cucurbita maxima (Rimu)	cmacmoB746
CmoCh04G000710	Cla003955	Watermelon (97103) v1	cmowmB708
CmoCh04G000710	Cla003404	Watermelon (97103) v1	cmowmB680
CmoCh04G000710	Csa5G627080	Cucumber (Chinese Long) v2	cmocuB744
CmoCh04G000710	MELO3C026437	Melon (DHL92) v3.5.1	cmomeB636
CmoCh04G000710	MELO3C005272	Melon (DHL92) v3.5.1	cmomeB629
CmoCh04G000710	ClCG07G005670	Watermelon (Charleston Gray)	cmowcgB676
CmoCh04G000710	ClCG01G006550	Watermelon (Charleston Gray)	cmowcgB638
CmoCh04G000710	CSPI05G27880	Wild cucumber (PI 183967)	cmocpiB753
CmoCh04G000710	Lsi04G011060	Bottle gourd (USVL1VR-Ls)	cmolsiB673
CmoCh04G000710	Cp4.1LG01g07490	Cucurbita pepo (Zucchini)	cmocpeB674
CmoCh04G000710	MELO3C026437.2	Melon (DHL92) v3.6.1	cmomedB724
CmoCh04G000710	CsaV3_5G037010	Cucumber (Chinese Long) v3	cmocucB0883
CmoCh04G000710	Cla97C01G006860	Watermelon (97103) v2	cmowmbB679
CmoCh04G000710	Cla97C07G133920	Watermelon (97103) v2	cmowmbB755
CmoCh04G000710	Bhi07G000337	Wax gourd	cmowgoB0889
CmoCh04G000710	Bhi12G000820	Wax gourd	cmowgoB0826
CmoCh04G000710	CsGy5G027280	Cucumber (Gy14) v2	cgybcmoB659
CmoCh04G000710	Carg22019	Silver-seed gourd	carcmoB0250

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmoCh04G000710	CmoCh04G022070	Cucurbita moschata (Rifu)	cmocmoB471

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh04G000710	Cucurbita moschata (Rifu)	cmocmoB464
CmoCh04G000710	Wild cucumber (PI 183967)	cmocpiB731
CmoCh04G000710	Melon (DHL92) v3.5.1	cmomeB700
CmoCh04G000710	Cucurbita pepo (Zucchini)	cmocpeB673
CmoCh04G000710	Melon (DHL92) v3.6.1	cmomedB794
CmoCh04G000710	Silver-seed gourd	carcmoB0924