Cp4.1LG01g07490 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g07490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPolcalcin Jun o
LocationCp4.1LG01 : 5520685 .. 5521248 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTCAAATCTCTTTTCTGCCGAAAGAAGAAGCCATTCGCCTCCGTCGATTCTCCGCTCGTCTCCGCGTCCACGACGCTAGGGTCTGGATCTTCATTGGAAGATTCACGGGCTCAGATAGCGGAACTCGAGGAGGTATTCAAGAAATTCGATGTGAATGGTGATGGAAAGATCTCTTCTTCGGAATTAGGCACGATTTTGGGGAGCCTAGGGCACAACGCTAAGGAGGAAGAGCTCGCGAAGATGATCGAGGAATTTGACGCTGATGGAGACGGATTCATCGATTTGCAGGAGTTCGTGGAGTTGAATACCAAAGAGATCGATCCTGAGGAGCTGTTGGAGAGTCTAAGAGAGGCGTTTTCGGTCTACGATATCGATGGAAACGGATCGATCACCGCCGAGGAATTGCACGAGGTTCTGAAGAGTTTGGGGGACGATTGCTCGGTAGAGGATTGTCGACAGATGATAAGTGGCGTCGATAGAAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGATGTCGACGGGACTCTCCGTCGCTGACGGCTGA

mRNA sequence

ATGGGATTCAAATCTCTTTTCTGCCGAAAGAAGAAGCCATTCGCCTCCGTCGATTCTCCGCTCGTCTCCGCGTCCACGACGCTAGGGTCTGGATCTTCATTGGAAGATTCACGGGCTCAGATAGCGGAACTCGAGGAGGTATTCAAGAAATTCGATGTGAATGGTGATGGAAAGATCTCTTCTTCGGAATTAGGCACGATTTTGGGGAGCCTAGGGCACAACGCTAAGGAGGAAGAGCTCGCGAAGATGATCGAGGAATTTGACGCTGATGGAGACGGATTCATCGATTTGCAGGAGTTCGTGGAGTTGAATACCAAAGAGATCGATCCTGAGGAGCTGTTGGAGAGTCTAAGAGAGGCGTTTTCGGTCTACGATATCGATGGAAACGGATCGATCACCGCCGAGGAATTGCACGAGGTTCTGAAGAGTTTGGGGGACGATTGCTCGGTAGAGGATTGTCGACAGATGATAAGTGGCGTCGATAGAAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGATGTCGACGGGACTCTCCGTCGCTGACGGCTGA

Coding sequence (CDS)

ATGGGATTCAAATCTCTTTTCTGCCGAAAGAAGAAGCCATTCGCCTCCGTCGATTCTCCGCTCGTCTCCGCGTCCACGACGCTAGGGTCTGGATCTTCATTGGAAGATTCACGGGCTCAGATAGCGGAACTCGAGGAGGTATTCAAGAAATTCGATGTGAATGGTGATGGAAAGATCTCTTCTTCGGAATTAGGCACGATTTTGGGGAGCCTAGGGCACAACGCTAAGGAGGAAGAGCTCGCGAAGATGATCGAGGAATTTGACGCTGATGGAGACGGATTCATCGATTTGCAGGAGTTCGTGGAGTTGAATACCAAAGAGATCGATCCTGAGGAGCTGTTGGAGAGTCTAAGAGAGGCGTTTTCGGTCTACGATATCGATGGAAACGGATCGATCACCGCCGAGGAATTGCACGAGGTTCTGAAGAGTTTGGGGGACGATTGCTCGGTAGAGGATTGTCGACAGATGATAAGTGGCGTCGATAGAAACGGCGACGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGATGTCGACGGGACTCTCCGTCGCTGACGGCTGA

Protein sequence

MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMSTGLSVADG
Homology
[Close]
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.4e-50
Identity = 96/140 (68.57%), Postives = 117/140 (83.57%), Query Frame = 1 

Query: 38  RAQIAELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDL 97
           + +I ELE VFKKFDVNGDGKISS ELG I+ SLGH   EEEL K I E D  GDG+I+ 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 98  QEFVELNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMI 157
           +EFVELNTK +D  ++LE+L++AFSVYDIDGNGSI+AEELHEVL+SLGD+CS+ +CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 158 SGVDRNGDGMISFEEFKVMM 178
            GVD++GDG I FEEFK+MM
Sbjct: 152 GGVDKDGDGTIDFEEFKIMM 171

[Close]
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-39
Identity = 94/178 (52.81%), Postives = 114/178 (64.04%), Query Frame = 1 

Query: 5   SLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKISSSEL 64
           +LF R+     S   PL  A    G GS          E+E VF+KFD NGDG+IS SEL
Sbjct: 8   ALFRRRS---GSKSPPLPQADPASGGGSPAPTPEE---EMERVFRKFDANGDGRISRSEL 67

Query: 65  GTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELN-TKEIDPEELLESLREAFSV 124
           G +  SLGH A ++ELA+M+ E DADGDGFI L EF  LN T   D   + E LR AF V
Sbjct: 68  GALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRV 127

Query: 125 YDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMSTG 182
           +D DGNG+I+A EL  VL  LG+  +V+ CR+MI GVD+NGDG+ISFEEFKVMM   G
Sbjct: 128 FDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGG 179

[Close]
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 5.0e-35
Identity = 77/139 (55.40%), Postives = 100/139 (71.94%), Query Frame = 1 

Query: 41  IAELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEF 100
           + ELEEVFKKFD NGDGKIS SEL  IL SLG +  E E+  M+EE DADGDG++ LQEF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 101 VELNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGV 160
           V+LN K       ++ L+ AF V+D D NGSI+A EL   L+S+G+ C++E+ + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 161 DRNGDGMISFEEFKVMMMS 180
           D+NGDG+IS EEF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

[Close]
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-34
Identity = 71/141 (50.35%), Postives = 101/141 (71.63%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           ++++VF++FD N DGKIS  EL  ++G+L  NA +EE   M++EFD DG+GFIDL EFV 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 103 LN--TKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGV 162
           L   + +      +  L+EAF +YD+D NG I+A ELH V+K+LG+ CS++DC++MI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 163 DRNGDGMISFEEFKVMMMSTG 182
           D +GDG + FEEFK MMM  G
Sbjct: 135 DSDGDGCVDFEEFKKMMMING 155

[Close]
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.7e-33
Identity = 71/136 (52.21%), Postives = 95/136 (69.85%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           E E VF+KFD NGDG+IS +EL  +  S+GH   ++E+A+M++E D+DGDG+I L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 103 LNTKEI-DPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVD 162
           ++     D     E LR AF V+D DGNG IT  EL  VL+ +G+  +V  CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 163 RNGDGMISFEEFKVMM 178
           RNGDG+I+FEEFK+MM
Sbjct: 175 RNGDGLINFEEFKLMM 190

[Close]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 5.1e-79
Identity = 158/185 (85.41%), Postives = 174/185 (94.05%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+S+TLGS SSL+DS +QI ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           S+ELG+I+ SLGHNA EEEL KMI+EFDADGDGFI+L EFVELNTKEIDPEELLE+LREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMISGVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180

Query: 181 GLSVA 186
           GLSVA
Sbjct: 181 GLSVA 183

[Close]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 7.1e-65
Identity = 133/179 (74.30%), Postives = 152/179 (84.92%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGFKSLF RKKK  +S DSPL   STTL     +  SR QIAELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL--GSTTL----PILGSRTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           SSELG+I+GSLG  A EEEL  MI+E DADGDG+IDL EF+ELNTK +D +E+LE+L++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMS 180
           FSVYDIDGNGSITAEELHEVLKSLGDDCS+ DCR+MI+GVD+NGDGMISF+EFKVMMMS
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 173

[Close]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match: W9SF34_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.0e-60
Identity = 124/180 (68.89%), Postives = 150/180 (83.33%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVS-ASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKI 60
           MGFKSLF R+KKP +S  +P  S A     S S   + RAQI ELE+VFKKFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  SSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLRE 120
           SSSELG I+GSLG  + EEEL KMI+E D+DGDGFIDL+EF+ELNTK +D +E+LE+L++
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMS 180
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+ +CR+MISGVD +GDG ISFEEFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

[Close]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-58
Identity = 123/190 (64.74%), Postives = 152/190 (80.00%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGS-GSSLEDS----------RAQIAELEEVFK 60
           MGF S+F RKKK  +  ++  ++ S  +G+ GSS   S          + Q+ ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEID 120
           KFDVNGDGKISSSELG+I+GSLGH   E+EL KMI+EFDADGDG I+ +EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMIS 180
            +E+LE+L++AFSVYDIDGNGSI+AEELHEVLKSLGD+CS+ +CR+MISGVD +GDGMI 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match: B9HW20_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 1.9e-57
Identity = 118/181 (65.19%), Postives = 146/181 (80.66%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPF---ASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDG 60
           MGFK+LF RKKK     +SVDSP    ++  GS S     R Q+ ELE+VFKKFDVNGDG
Sbjct: 1   MGFKALFNRKKKSKKSDSSVDSPSAIVASISGSKSPSIRLRPQVEELEQVFKKFDVNGDG 60

Query: 61  KISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESL 120
           KISS+ELG+I+ +LGH A E+EL  MI EFDADGDGFIDLQEFV LNT+ +D  E++E+L
Sbjct: 61  KISSAELGSIMANLGHEATEDELQTMITEFDADGDGFIDLQEFVALNTQGVDTNEVMENL 120

Query: 121 REAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMM 179
           ++AFSVYD+DGNGSI+AEELH+V+ SLG+ CS+ +CR++ISGVD +GDGMI FEEFKVMM
Sbjct: 121 KDAFSVYDVDGNGSISAEELHKVMASLGEPCSMSECRKIISGVDSDGDGMIDFEEFKVMM 180

[Close]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 200.7 bits (509), Expect = 8.1e-52
Identity = 96/140 (68.57%), Postives = 117/140 (83.57%), Query Frame = 1 

Query: 38  RAQIAELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDL 97
           + +I ELE VFKKFDVNGDGKISS ELG I+ SLGH   EEEL K I E D  GDG+I+ 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 98  QEFVELNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMI 157
           +EFVELNTK +D  ++LE+L++AFSVYDIDGNGSI+AEELHEVL+SLGD+CS+ +CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 158 SGVDRNGDGMISFEEFKVMM 178
            GVD++GDG I FEEFK+MM
Sbjct: 152 GGVDKDGDGTIDFEEFKIMM 171

[Close]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 147.1 bits (370), Expect = 1.1e-35
Identity = 71/141 (50.35%), Postives = 101/141 (71.63%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           ++++VF++FD N DGKIS  EL  ++G+L  NA +EE   M++EFD DG+GFIDL EFV 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 103 LN--TKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGV 162
           L   + +      +  L+EAF +YD+D NG I+A ELH V+K+LG+ CS++DC++MI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 163 DRNGDGMISFEEFKVMMMSTG 182
           D +GDG + FEEFK MMM  G
Sbjct: 135 DSDGDGCVDFEEFKKMMMING 155

[Close]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 141.4 bits (355), Expect = 5.8e-34
Identity = 71/143 (49.65%), Postives = 102/143 (71.33%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A  EE   M+++FD DG+GFIDL EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMIS 162
           L    I    +    +  L+EAF +YD+DGNG I+A+ELH V+K+LG+ CSV+DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 GVDRNGDGMISFEEFKVMMMSTG 182
            VD +GDG ++F+EFK MM + G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNGG 159

[Close]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 132.5 bits (332), Expect = 2.7e-31
Identity = 71/145 (48.97%), Postives = 96/145 (66.21%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           EL++VF +FD NGDGKIS  ELG +  ++G +  E EL +++EE D D DG+I+L EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 103 LNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDR 162
           L        E+    R+AF +YD D NG I+A ELH+VL  LG  CSVEDC +MI  VD 
Sbjct: 83  LCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 163 NGDGMISFEEFKVMMMSTGLSVADG 188
           +GDG ++FEEF+ MM S+ L  ++G
Sbjct: 143 DGDGNVNFEEFQKMMTSSSLLNSNG 163

[Close]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 131.7 bits (330), Expect = 4.6e-31
Identity = 70/140 (50.00%), Postives = 94/140 (67.14%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVE 102
           EL++VF KFD NGDGKIS SELG +  S+G +  EEEL ++++E D D DGFI+ +EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 103 LNTKEIDPEELLESLREAFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDR 162
           +        E+    REAF +YD + NG I++ E+H+VL  LG  CSVEDC +MI  VD 
Sbjct: 80  ICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 163 NGDGMISFEEFKVMMMSTGL 183
           +GDG ++FEEF+ MM S  L
Sbjct: 140 DGDGNVNFEEFQKMMSSPEL 155

[Close]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 304.7 bits (779), Expect = 1.1e-79
Identity = 160/187 (85.56%), Postives = 175/187 (93.58%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+S+TLGS SSL+ S +QIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           S+ELG+I+ SLGHNA EEEL KMIEEFDADGDGFI+L EFVELNTKEIDPEELLE+LREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMISGVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180

Query: 181 GLSVADG 188
           GLSVA G
Sbjct: 181 GLSVAGG 185

[Close]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 7.3e-79
Identity = 158/185 (85.41%), Postives = 174/185 (94.05%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+S+TLGS SSL+DS +QI ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           S+ELG+I+ SLGHNA EEEL KMI+EFDADGDGFI+L EFVELNTKEIDPEELLE+LREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMISGVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180

Query: 181 GLSVA 186
           GLSVA
Sbjct: 181 GLSVA 183

[Close]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 255.0 bits (650), Expect = 1.0e-64
Identity = 133/179 (74.30%), Postives = 152/179 (84.92%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGFKSLF RKKK  +S DSPL   STTL     +  SR QIAELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL--GSTTL----PILGSRTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           SSELG+I+GSLG  A EEEL  MI+E DADGDG+IDL EF+ELNTK +D +E+LE+L++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMS 180
           FSVYDIDGNGSITAEELHEVLKSLGDDCS+ DCR+MI+GVD+NGDGMISF+EFKVMMMS
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 173

[Close]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 253.1 bits (645), Expect = 3.9e-64
Identity = 133/179 (74.30%), Postives = 150/179 (83.80%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVSASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60
           MGFKSLF RKKK   S DSPL   STTL     +  SR QIAELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPL--GSTTL----PIFGSRTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLREA 120
           SSELG+I+GSLG  A EEEL  MI+E DADGDG+IDL EF+ELNTK +D  E+LE+L++A
Sbjct: 61  SSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMS 180
           FSVYDIDGNGSITAEELHEVLKSLGDDCS+ DCR+MI+GVD+NGDGMISF+EFKVMMMS
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 173

[Close]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match: gi|703130743|ref|XP_010104706.1| (putative calcium-binding protein CML25 [Morus notabilis])

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-59
Identity = 124/180 (68.89%), Postives = 150/180 (83.33%), Query Frame = 1 

Query: 1   MGFKSLFCRKKKPFASVDSPLVS-ASTTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKI 60
           MGFKSLF R+KKP +S  +P  S A     S S   + RAQI ELE+VFKKFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  SSSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLESLRE 120
           SSSELG I+GSLG  + EEEL KMI+E D+DGDGFIDL+EF+ELNTK +D +E+LE+L++
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMS 180
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+ +CR+MISGVD +GDG ISFEEFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g07490 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML25_ARATH1.4e-5068.57Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ1.1e-3952.81Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
POLC2_JUNOX5.0e-3555.40Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2[more]
CML23_ARATH1.9e-3450.35Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML15_ORYSJ2.7e-3352.21Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ... [more]
BLAST of Cp4.1LG01g07490 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KX11_CUCSA5.1e-7985.41Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1[more]
A0A0A0KST2_CUCSA7.1e-6574.30Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1[more]
W9SF34_9ROSA9.0e-6068.89Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV... [more]
A0A061E9N9_THECC1.3e-5864.74Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1[more]
B9HW20_POPTR1.9e-5765.19Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2[more]
BLAST of Cp4.1LG01g07490 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.18.1e-5268.57 EF hand calcium-binding protein family[more]
AT1G66400.11.1e-3550.35 calmodulin like 23[more]
AT5G37770.15.8e-3449.65 EF hand calcium-binding protein family[more]
AT1G18210.12.7e-3148.97 Calcium-binding EF-hand family protein[more]
AT1G73630.14.6e-3150.00 EF hand calcium-binding protein family[more]
BLAST of Cp4.1LG01g07490 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659129528|ref|XP_008464718.1|1.1e-7985.56PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|449449242|ref|XP_004142374.1|7.3e-7985.41PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|778703916|ref|XP_011655449.1|1.0e-6474.30PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|659072787|ref|XP_008467096.1|3.9e-6474.30PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|703130743|ref|XP_010104706.1|1.3e-5968.89putative calcium-binding protein CML25 [Morus notabilis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0048767 root hair elongation
biological_process GO:0009409 response to cold
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g07490.1Cp4.1LG01g07490.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 43..103
score: 1.3
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 129..177
score: 4.8
IPR002048EF-hand domainSMARTSM00054efh_1coord: 152..180
score: 1.0E-6coord: 43..71
score: 6.0E-7coord: 79..107
score: 5.9E-5coord: 116..144
score: 3.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 148..183
score: 14.011coord: 39..74
score: 15.992coord: 75..110
score: 14.011coord: 112..147
score: 15
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 37..179
score: 1.5
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 37..179
score: 4.91
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 52..64
score: -coord: 161..173
score: -coord: 88..100
score: -coord: 125..137
scor
NoneNo IPR availableunknownCoilCoilcoord: 34..54
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 34..178
score: 2.1
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 34..178
score: 2.1