CmaCh04G000750 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G000750
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Calcium binding family protein
Location	Cma_Chr04 : 357037 .. 357597 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

mRNA sequence

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Coding sequence (CDS)

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCACCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGCATGGACCCCGCTCCTCATCCGGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTAAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCCAAGATGATCGAGGTATTCGACGCGGATGGCGATGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGAGAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAACGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGTTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGCCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Protein sequence

MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVAGG

Homology

[Close]

BLAST of CmaCh04G000750 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-50
Identity = 101/159 (63.52%), Postives = 127/159 (79.87%), Query Frame = 1


Query: 22  VSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELA 81
           + + S +  ++ SG +  R  ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL 
Sbjct: 18  IGADSPYLQKARSGKTEIR--ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELE 77

Query: 82  KMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVM 141
           K I   D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+
Sbjct: 78  KAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVL 137

Query: 142 RSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           RSLGD+CS+A+CR+MI  VD++GDG I F+EFK+MM+ G
Sbjct: 138 RSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmaCh04G000750 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 4.3e-39
Identity = 87/157 (55.41%), Postives = 112/157 (71.34%), Query Frame = 1


Query: 30  PRSSSGD-SHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFD 89
           P S  G  + +   E+E VF+KFD NGDG+IS +ELG++  SLGH AT++ELA+M+   D
Sbjct: 26  PASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEAD 85

Query: 90  ADGDGFINLQEFVELN-TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDD 149
           ADGDGFI+L EF  LN T   D   + E+LR AF V+D DGNG+ISA EL +V+  LG+ 
Sbjct: 86  ADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEK 145

Query: 150 CSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVA 185
            +V  CR+MI  VD+NGDGLISF+EFKVMM+ G S A
Sbjct: 146 ATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGSFA 182

[Close]

BLAST of CmaCh04G000750 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.8e-35
Identity = 73/139 (52.52%), Postives = 102/139 (73.38%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+A+M++  D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 103 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVD 162
           ++     D     E+LR AF V+D DGNG I+  EL +V+R +G+  +VA CR+MI  VD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 163 RNGDGLISFDEFKVMMSAG 181
           RNGDGLI+F+EFK+MM+AG
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]

BLAST of CmaCh04G000750 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-33
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 AVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmaCh04G000750 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.0e-32
Identity = 74/151 (49.01%), Postives = 98/151 (64.90%), Query Frame = 1


Query: 42  AELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFV 101
           AELE+VF+++D NGDGKIS+ EL S++ +LG      E+ +M++  D+D DGF++L EF 
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 102 ELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVMRSLGDDCS 161
             +               D E   E  LREAF +YD D NG ISA ELH+V+R LGD CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 162 VADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           VADC +MI +VD +GDG ++FDEFK MM  G
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]

BLAST of CmaCh04G000750 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 5.8e-75
Identity = 154/185 (83.24%), Postives = 163/185 (88.11%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]

BLAST of CmaCh04G000750 vs. TrEMBL
Match: A0A151S400_CAJCA (Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.0e-55
Identity = 119/187 (63.64%), Postives = 144/187 (77.01%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHG-PRSSSGDSHSRNAE-LEEVFKKFDVNGDGK 60
           M F+ LFC K+K   + +SS  S+ ST   P +  G    R AE LE VFKKFDVNGDGK
Sbjct: 1   MGFRSLFCRKRKMLKTTSSSSASTSSTSALPTAIPGSGRGRVAEELEYVFKKFDVNGDGK 60

Query: 61  ISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLR 120
           ISS+ELGS+M SLG  ATEEEL+KMI   DADGDG I+L+EF+ELNTK +DP+E+LENLR
Sbjct: 61  ISSSELGSMMRSLGQAATEEELSKMIGEVDADGDGHISLEEFIELNTKGVDPDEVLENLR 120

Query: 121 EAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMS 180
           +AFS++DIDGNGSI+ EELH VM SLGD CSV DCR+MI+ VD +GDG I FDEFK+MM+
Sbjct: 121 DAFSIFDIDGNGSITVEELHMVMASLGDQCSVDDCRKMIAGVDADGDGTIDFDEFKIMMT 180

Query: 181 AGLSVAG 186
           A  S +G
Sbjct: 181 ASASASG 187

[Close]

BLAST of CmaCh04G000750 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.3e-55
Identity = 119/180 (66.11%), Postives = 144/180 (80.00%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   S    ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGS---RTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+LENL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+ VD+NGDG+ISFDEFKVMM +G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]

BLAST of CmaCh04G000750 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 6.7e-55
Identity = 121/191 (63.35%), Postives = 146/191 (76.44%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFT-SVTSSIVSSPSTHGPRSSSGDSHSRNA----------ELEEVFK 60
           M F  +F  KKK  + S  S++  SP+     SS   S  R            ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEID 120
           KFDVNGDGKISS+ELGSIMGSLGHK +E+EL KMI+ FDADGDG IN +EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLIS 180
            +E+LENL++AFSVYDIDGNGSISAEELH+V++SLGD+CS+A+CR+MIS VD +GDG+I 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]

BLAST of CmaCh04G000750 vs. TrEMBL
Match: A0A067KPP2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 8.7e-55
Identity = 119/184 (64.67%), Postives = 139/184 (75.54%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSR----NAELEEVFKKFDVNGD 60
           M FK LF  KK   +S +S +  + S   P S SG    R      EL+ VF KFD NGD
Sbjct: 1   MGFKALFNRKKSKKSSDSSVVSPAGSPPDPLSRSGSQSLRLKHQTDELQHVFNKFDANGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLEN 120
           GKISSAELGSIM SLGHKAT+EEL KMI  FDADGDGFI+ QEFVELNT+ +D  E +EN
Sbjct: 61  GKISSAELGSIMASLGHKATDEELRKMIMEFDADGDGFIDFQEFVELNTQGVDTNEAMEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVM 180
           LR+AFSVYDIDGNGSISAEELH+VM SLG+ CS+A+CR+MIS VD +GDG+I F+EFKVM
Sbjct: 121 LRDAFSVYDIDGNGSISAEELHRVMGSLGESCSIAECRRMISGVDGDGDGMIDFEEFKVM 180

[Close]

BLAST of CmaCh04G000750 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 201.1 bits (510), Expect = 6.2e-52
Identity = 101/159 (63.52%), Postives = 127/159 (79.87%), Query Frame = 1


Query: 22  VSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELA 81
           + + S +  ++ SG +  R  ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL 
Sbjct: 18  IGADSPYLQKARSGKTEIR--ELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELE 77

Query: 82  KMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVM 141
           K I   D  GDG+IN +EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+V+
Sbjct: 78  KAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVL 137

Query: 142 RSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 181
           RSLGD+CS+A+CR+MI  VD++GDG I F+EFK+MM+ G
Sbjct: 138 RSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CmaCh04G000750 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 142.1 bits (357), Expect = 3.4e-34
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 AVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of CmaCh04G000750 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 137.5 bits (345), Expect = 8.4e-33
Identity = 66/137 (48.18%), Postives = 98/137 (71.53%), Query Frame = 1


Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD N DGKIS  EL  ++G+L   A++EE   M++ FD DG+GFI+L EFV 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 103 LN--TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAV 162
           L   + +      + +L+EAF +YD+D NG ISA ELH VM++LG+ CS+ DC++MI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 163 DRNGDGLISFDEFKVMM 178
           D +GDG + F+EFK MM
Sbjct: 135 DSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmaCh04G000750 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 131.0 bits (328), Expect = 7.8e-31
Identity = 72/160 (45.00%), Postives = 103/160 (64.38%), Query Frame = 1


Query: 26  STHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIE 85
           +T+   ++   + S + EL++VF KFD NGDGKIS +ELG++  S+G   TEEEL ++++
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 86  VFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMRSLG 145
             D D DGFIN +EF  +        E+    REAF +YD + NG IS+ E+HKV+  LG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 146 DDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAGLSVAG 186
             CSV DC +MI  VD +GDG ++F+EF+ MMS+   V G
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKG 158

[Close]

BLAST of CmaCh04G000750 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 127.5 bits (319), Expect = 8.7e-30
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 1


Query: 23  SSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAK 82
           ++P T  P  ++ D  +   EL++VF +FD NGDGKIS  ELG +  ++G   TE EL +
Sbjct: 4   ANPETAKPTPATVDM-ANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 63

Query: 83  MIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVMR 142
           ++E  D D DG+INL EF  L        E+    R+AF +YD D NG ISA ELH+V+ 
Sbjct: 64  VLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLN 123

Query: 143 SLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSA 180
            LG  CSV DC +MI  VD +GDG ++F+EF+ MM++
Sbjct: 124 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTS 155

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 292.0 bits (746), Expect = 7.5e-76
Identity = 157/186 (84.41%), Postives = 164/186 (88.17%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS   S S+ AELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMIE FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV+RSLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 185

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 288.5 bits (737), Expect = 8.3e-75
Identity = 154/185 (83.24%), Postives = 163/185 (88.11%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELLENLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMIS VD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: gi|1012338237|gb|KYP49494.1| (putative calcium-binding protein CML25 [Cajanus cajan])

HSP 1 Score: 224.6 bits (571), Expect = 1.5e-55
Identity = 119/187 (63.64%), Postives = 144/187 (77.01%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHG-PRSSSGDSHSRNAE-LEEVFKKFDVNGDGK 60
           M F+ LFC K+K   + +SS  S+ ST   P +  G    R AE LE VFKKFDVNGDGK
Sbjct: 1   MGFRSLFCRKRKMLKTTSSSSASTSSTSALPTAIPGSGRGRVAEELEYVFKKFDVNGDGK 60

Query: 61  ISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLR 120
           ISS+ELGS+M SLG  ATEEEL+KMI   DADGDG I+L+EF+ELNTK +DP+E+LENLR
Sbjct: 61  ISSSELGSMMRSLGQAATEEELSKMIGEVDADGDGHISLEEFIELNTKGVDPDEVLENLR 120

Query: 121 EAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMS 180
           +AFS++DIDGNGSI+ EELH VM SLGD CSV DCR+MI+ VD +GDG I FDEFK+MM+
Sbjct: 121 DAFSIFDIDGNGSITVEELHMVMASLGDQCSVDDCRKMIAGVDADGDGTIDFDEFKIMMT 180

Query: 181 AGLSVAG 186
           A  S +G
Sbjct: 181 ASASASG 187

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 224.2 bits (570), Expect = 1.9e-55
Identity = 120/184 (65.22%), Postives = 143/184 (77.72%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSG----DSHSRNAELEEVFKKFDVNGD 60
           M FK LF  KKK          S PST  P  S+      S ++ AELE+VF KFDVNGD
Sbjct: 1   MGFKSLFSRKKK----------SHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLEN 120
           GKI S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D  E+LEN
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVM 180
           L++AFSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+ VD+NGDG+ISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVM 174

[Close]

BLAST of CmaCh04G000750 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 223.4 bits (568), Expect = 3.3e-55
Identity = 119/180 (66.11%), Postives = 144/180 (80.00%), Query Frame = 1


Query: 1   MRFKCLFCWKKKPFTSVTSSIVSSPSTHGPRSSSGDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   S    ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGS---RTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLENLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+LENL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISAVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+ VD+NGDG+ISFDEFKVMM +G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

The following BLAST results are available for this feature:

BLAST of CmaCh04G000750 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML25_ARATH	1.1e-50	63.52	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML10_ORYSJ	4.3e-39	55.41	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]
CML15_ORYSJ	3.8e-35	52.52	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ...	[more]
CML24_ARATH	6.0e-33	51.41	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML18_ORYSJ	1.0e-32	49.01	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ...	[more]

BLAST of CmaCh04G000750 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX11_CUCSA	5.8e-75	83.24	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1	[more]
A0A151S400_CAJCA	1.0e-55	63.64	Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1	[more]
A0A0A0KST2_CUCSA	2.3e-55	66.11	Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1	[more]
A0A061E9N9_THECC	6.7e-55	63.35	Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1	[more]
A0A067KPP2_JATCU	8.7e-55	64.67	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1	[more]

BLAST of CmaCh04G000750 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	6.2e-52	63.52	EF hand calcium-binding protein family	[more]
AT5G37770.1	3.4e-34	51.41	EF hand calcium-binding protein family	[more]
AT1G66400.1	8.4e-33	48.18	calmodulin like 23	[more]
AT1G73630.1	7.8e-31	45.00	EF hand calcium-binding protein family	[more]
AT1G18210.1	8.7e-30	45.22	Calcium-binding EF-hand family protein	[more]

BLAST of CmaCh04G000750 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659129528\|ref\|XP_008464718.1\|	7.5e-76	84.41	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]
gi\|449449242\|ref\|XP_004142374.1\|	8.3e-75	83.24	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus]	[more]
gi\|1012338237\|gb\|KYP49494.1\|	1.5e-55	63.64	putative calcium-binding protein CML25 [Cajanus cajan]	[more]
gi\|659072787\|ref\|XP_008467096.1\|	1.9e-55	65.22	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]
gi\|778703916\|ref\|XP_011655449.1\|	3.3e-55	66.11	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0048767	root hair elongation
biological_process	GO:0009409	response to cold
biological_process	GO:0008150	biological_process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G000750.1	CmaCh04G000750.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 43..103 score: 7.1E-13coord: 115..177 score: 3.1
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 152..180 score: 3.7E-6coord: 79..107 score: 7.2E-5coord: 43..71 score: 4.7E-7coord: 116..144 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 148..183 score: 13.37coord: 112..147 score: 15.741coord: 39..74 score: 15.797coord: 75..110 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 37..178 score: 2.3
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 39..178 score: 5.62
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137 score: -coord: 161..173 score: -coord: 88..100 score: -coord: 52..64 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 43..180 score: 1.5
None	No IPR available	PANTHER	PTHR10891:SF605	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 43..180 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G000750	Cucsa.029820	Cucumber (Gy14) v1	cgycmaB0002
CmaCh04G000750	Cla003955	Watermelon (97103) v1	cmawmB713
CmaCh04G000750	Cla003404	Watermelon (97103) v1	cmawmB688
CmaCh04G000750	Csa5G627080	Cucumber (Chinese Long) v2	cmacuB756
CmaCh04G000750	MELO3C005272	Melon (DHL92) v3.5.1	cmameB639
CmaCh04G000750	MELO3C026437	Melon (DHL92) v3.5.1	cmameB648
CmaCh04G000750	ClCG01G006550	Watermelon (Charleston Gray)	cmawcgB636
CmaCh04G000750	ClCG07G005670	Watermelon (Charleston Gray)	cmawcgB674
CmaCh04G000750	CSPI05G27880	Wild cucumber (PI 183967)	cmacpiB768
CmaCh04G000750	CmoCh04G000710	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G000750	Lsi04G011060	Bottle gourd (USVL1VR-Ls)	cmalsiB680
CmaCh04G000750	Cp4.1LG01g07490	Cucurbita pepo (Zucchini)	cmacpeB722
CmaCh04G000750	MELO3C026437.2	Melon (DHL92) v3.6.1	cmamedB732
CmaCh04G000750	CsaV3_5G037010	Cucumber (Chinese Long) v3	cmacucB0894
CmaCh04G000750	Cla97C07G133920	Watermelon (97103) v2	cmawmbB781
CmaCh04G000750	Cla97C01G006860	Watermelon (97103) v2	cmawmbB709
CmaCh04G000750	Bhi07G000337	Wax gourd	cmawgoB0890
CmaCh04G000750	Bhi12G000820	Wax gourd	cmawgoB0830
CmaCh04G000750	CsGy5G027280	Cucumber (Gy14) v2	cgybcmaB682
CmaCh04G000750	Carg22019	Silver-seed gourd	carcmaB0242

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh04G000750	CmaCh04G010210	Cucurbita maxima (Rimu)	cmacmaB531
CmaCh04G000750	CmaCh04G021050	Cucurbita maxima (Rimu)	cmacmaB539

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G000750	Cucurbita moschata (Rifu)	cmacmoB730
CmaCh04G000750	Wild cucumber (PI 183967)	cmacpiB740
CmaCh04G000750	Melon (DHL92) v3.5.1	cmameB708
CmaCh04G000750	Cucurbita pepo (Zucchini)	cmacpeB720
CmaCh04G000750	Melon (DHL92) v3.6.1	cmamedB797
CmaCh04G000750	Silver-seed gourd	carcmaB0949