MELO3C005272 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C005272
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Calcium binding family protein
Location	chr9 : 18760664 .. 18761566 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCCAAAGAAAATTCTAATCCCCTTTATCCCTCTAAATTTGGAGGAAAAAAACAAATTCCATGGAATTTCAATCTGTTACAATCTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCCCAAATCCCCCTCCTTATATATCTCTAATTCCTCCCCTATTCCTTGTCTCTAATCCATCAATTTTCCCCCTCTTTTTCGATTTAGTATATTCAATCAATCTCTTCTCATTATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATCCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTTTCGGATCCCGTACCCAAATCGCCGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCACAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCAATGAGGTGCTTGAGAATCTGAAAGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACCGGCGTTGATAAGAACGGCGACGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGGTTTAATGGTTAATTATATTAAATTAATCCTAATTGATGATTTTATGAGTAATTAGCTTATTTTGCTAACATTTACTGTTTCTTTATCCTATAATTAGGAATTATTTGTTTGTAATTTATTTTTAATTATAATTC

mRNA sequence

AACCCAAAGAAAATTCTAATCCCCTTTATCCCTCTAAATTTGGAGGAAAAAAACAAATTCCATGGAATTTCAATCTGTTACAATCTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCCCAAATCCCCCTCCTTATATATCTCTAATTCCTCCCCTATTCCTTGTCTCTAATCCATCAATTTTCCCCCTCTTTTTCGATTTAGTATATTCAATCAATCTCTTCTCATTATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATCCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTTTCGGATCCCGTACCCAAATCGCCGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCACAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCAATGAGGTGCTTGAGAATCTGAAAGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACCGGCGTTGATAAGAACGGCGACGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGGTTTAATGGTTAATTATATTAAATTAATCCTAATTGATGATTTTATGAGTAATTAGCTTATTTTGCTAACATTTACTGTTTCTTTATCCTATAATTAGGAATTATTTGTTTGTAATTTATTTTTAATTATAATTC

Coding sequence (CDS)

ATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATCCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTTTCGGATCCCGTACCCAAATCGCCGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCACAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCAATGAGGTGCTTGAGAATCTGAAAGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACCGGCGTTGATAAGAACGGCGACGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGGTTTAATGGTTAA

Protein sequence

MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGFNG*

Homology

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BLAST of MELO3C005272 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 2.9e-56
Identity = 106/151 (70.20%), Postives = 121/151 (80.13%), Query Frame = 1


Query: 32  RTQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDL 91
           +T+I ELE VF KFDVNGDGKI S ELG+IM SLG    EEEL   I E+D  GDGYI+ 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 92  DEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMI 151
           +EF+ELNTKG+D N+VLENLKDAFSVYDIDGNGSI+AEELHEVL+SLGD+CS+A+CRKMI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 152 TGVDKNGDGMISFDEFKVMMMSGSRSHGFNG 183
            GVDK+GDG I F+EFK+MM  GSR     G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMGSRRDNVMG 182

[Close]

BLAST of MELO3C005272 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.1e-38
Identity = 80/139 (57.55%), Postives = 100/139 (71.94%), Query Frame = 1


Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIE 96
           E+E+VF KFD NGDG+I  SELG++  SLG  AT++EL  M+ E DADGDG+I LDEF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 97  LN-TKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD 156
           LN T   D+  V E+L+ AF V+D DGNG+I+A EL  VL  LG+  ++  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 157 KNGDGMISFDEFKVMMMSG 175
           +NGDG+ISF+EFKVMM  G
Sbjct: 160 QNGDGLISFEEFKVMMAGG 178

[Close]

BLAST of MELO3C005272 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 3.1e-34
Identity = 69/142 (48.59%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 97  LNTKGV----DSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 156
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 157 GVDKNGDGMISFDEFKVMMMSG 175
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

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BLAST of MELO3C005272 vs. Swiss-Prot
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 3.1e-34
Identity = 72/139 (51.80%), Postives = 96/139 (69.06%), Query Frame = 1


Query: 35  IAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEF 94
           + ELE+VF KFD NGDGKI  SEL  I+ SLG    E E+  M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 95  IELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 154
           ++LN KG      +++LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 155 DKNGDGMISFDEFKVMMMS 174
           DKNGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

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BLAST of MELO3C005272 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-33
Identity = 68/138 (49.28%), Postives = 96/138 (69.57%), Query Frame = 1


Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD N DGKI   EL  ++G+L   A++EE   M+KE D DG+G+IDLDEF+ 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 97  LNTKGVDS--NEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 156
           L      S  N  + +LK+AF +YD+D NG I+A ELH V+K+LG+ CS+ DC++MI  V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 157 DKNGDGMISFDEFKVMMM 173
           D +GDG + F+EFK MMM
Sbjct: 135 DSDGDGCVDFEEFKKMMM 152

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BLAST of MELO3C005272 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-94
Identity = 177/182 (97.25%), Postives = 178/182 (97.80%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSH STDSPLGSTTLPI GSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120
           IMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKGVDS+EVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRS GF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

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BLAST of MELO3C005272 vs. TrEMBL
Match: W9SF34_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 3.7e-66
Identity = 135/183 (73.77%), Postives = 153/183 (83.61%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRT-------QIAELEQVFNKFDVNGDGKI 60
           MGFKSLF R+KK   ST +P  S     F SR+       QI ELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKD 120
            SSELG+IMGSLGQP+ EEEL  MIKEVD+DGDG+IDL EFIELNTKGVDS+EVLENLKD
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 177
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+A+CRKMI+GVD +GDG ISF+EFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

[Close]

BLAST of MELO3C005272 vs. TrEMBL
Match: M5XF25_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012032mg PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 4.8e-66
Identity = 133/179 (74.30%), Postives = 152/179 (84.92%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPS-TDSPLGSTTL--PIFGSRTQIAELEQVFNKFDVNGDGKICSSE 60
           MGFKSLFSRKKKS PS T  PL +T    P F SR QIAEL+QVF KFDVNGDGKI SSE
Sbjct: 1   MGFKSLFSRKKKSIPSATALPLTTTPSRSPSFSSRAQIAELDQVFKKFDVNGDGKISSSE 60

Query: 61  LGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSV 120
           LG+IM SLGQPA EEEL +MI EVD+DGDG+ID  EF+ELNT GVD  E LENLKDAFSV
Sbjct: 61  LGAIMSSLGQPANEEELSHMILEVDSDGDGFIDFKEFVELNTNGVDQAEALENLKDAFSV 120

Query: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           YDIDGNGSI+AEEL++VL+SLGD+CS+A+C+KMI+ VD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 YDIDGNGSISAEELYKVLRSLGDECSIAECKKMISAVDSDGDGMINFEEFKVMMMMGSR 179

[Close]

BLAST of MELO3C005272 vs. TrEMBL
Match: A0A0D2R7V3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G172400 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.9e-62
Identity = 126/184 (68.48%), Postives = 153/184 (83.15%), Query Frame = 1


Query: 1   MGFKSLFSRKKK--SHPSTDSPLGSTTLPIF-------GSRTQIAELEQVFNKFDVNGDG 60
           MGF S+F+RKKK  S   TDSP  S++  +F        ++TQ+ E+E+VF KFD NGDG
Sbjct: 1   MGFGSIFNRKKKLRSRNLTDSPTVSSSGSLFLQSPVRNFTQTQLQEIEEVFKKFDANGDG 60

Query: 61  KICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENL 120
           KI +SELGSIM SLGQ  ++EELHNMIKE DADGDG+I+ +EFIELNTKGVDS EVLENL
Sbjct: 61  KISASELGSIMKSLGQQPSDEELHNMIKEFDADGDGFINFNEFIELNTKGVDSEEVLENL 120

Query: 121 KDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMM 176
           KDAFSVYDIDGNG I+AEELHEVLKSLG++CS+A+CRKMI+GVD +G+GMI F+EFKVMM
Sbjct: 121 KDAFSVYDIDGNGWISAEELHEVLKSLGEECSIAECRKMISGVDNDGNGMIDFEEFKVMM 180

[Close]

BLAST of MELO3C005272 vs. TrEMBL
Match: K7KRI8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G238400 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 2.5e-62
Identity = 130/177 (73.45%), Postives = 153/177 (86.44%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIA-ELEQVFNKFDVNGDGKICSSELG 60
           MG KSLF+R K +  S+D+   ST+L +  SRT++A ELEQVFNKFDVNGDGKI +SELG
Sbjct: 1   MGLKSLFNRNKDAI-SSDTASRSTSLSV-RSRTRMADELEQVFNKFDVNGDGKISASELG 60

Query: 61  SIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYD 120
           SIMGSLGQPATE EL NMI+EVD DGDG I L EFIELNTKGVDS+EVLENLKDAF+V+D
Sbjct: 61  SIMGSLGQPATELELDNMIREVDGDGDGCISLPEFIELNTKGVDSDEVLENLKDAFAVFD 120

Query: 121 IDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           IDGNGSITAEEL+ V++SLG+DCSLA+CR+MI+GVD +GDG I F+EF+VMMM GSR
Sbjct: 121 IDGNGSITAEELNTVMRSLGEDCSLAECRRMISGVDGDGDGTIDFEEFRVMMMMGSR 175

[Close]

BLAST of MELO3C005272 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 219.5 bits (558), Expect = 1.6e-57
Identity = 106/151 (70.20%), Postives = 121/151 (80.13%), Query Frame = 1


Query: 32  RTQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDL 91
           +T+I ELE VF KFDVNGDGKI S ELG+IM SLG    EEEL   I E+D  GDGYI+ 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 92  DEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMI 151
           +EF+ELNTKG+D N+VLENLKDAFSVYDIDGNGSI+AEELHEVL+SLGD+CS+A+CRKMI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 152 TGVDKNGDGMISFDEFKVMMMSGSRSHGFNG 183
            GVDK+GDG I F+EFK+MM  GSR     G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMGSRRDNVMG 182

[Close]

BLAST of MELO3C005272 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 146.4 bits (368), Expect = 1.8e-35
Identity = 69/142 (48.59%), Postives = 102/142 (71.83%), Query Frame = 1


Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 97  LNTKGV----DSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 156
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 157 GVDKNGDGMISFDEFKVMMMSG 175
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of MELO3C005272 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 143.7 bits (361), Expect = 1.1e-34
Identity = 68/138 (49.28%), Postives = 96/138 (69.57%), Query Frame = 1


Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD N DGKI   EL  ++G+L   A++EE   M+KE D DG+G+IDLDEF+ 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 97  LNTKGVDS--NEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 156
           L      S  N  + +LK+AF +YD+D NG I+A ELH V+K+LG+ CS+ DC++MI  V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 157 DKNGDGMISFDEFKVMMM 173
           D +GDG + F+EFK MMM
Sbjct: 135 DSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of MELO3C005272 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 136.0 bits (341), Expect = 2.4e-32
Identity = 73/163 (44.79%), Postives = 99/163 (60.74%), Query Frame = 1


Query: 13  SHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEE 72
           ++P T  P  +T            EL++VF++FD NGDGKI   ELG +  ++G   TE 
Sbjct: 4   ANPETAKPTPATV-----DMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTET 63

Query: 73  ELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELH 132
           EL+ +++EVD D DGYI+LDEF  L      + E+    +DAF +YD D NG I+A ELH
Sbjct: 64  ELNRVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELH 123

Query: 133 EVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGS 176
           +VL  LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 124 QVLNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of MELO3C005272 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 128.6 bits (322), Expect = 3.8e-30
Identity = 71/173 (41.04%), Postives = 102/173 (58.96%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           M   +L S  K + PSTD                  EL++VF+KFD NGDGKI  SELG+
Sbjct: 1   MANTNLESTNKSTTPSTDM-----------------ELKKVFDKFDANGDGKISVSELGN 60

Query: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120
           +  S+G   TEEEL+ ++ E+D D DG+I+ +EF  +      + E+    ++AF +YD 
Sbjct: 61  VFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFATICRSSSSAVEI----REAFDLYDQ 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 174
           + NG I++ E+H+VL  LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S
Sbjct: 121 NKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSS 152

[Close]

BLAST of MELO3C005272 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 367.5 bits (942), Expect = 1.4e-98
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120
           IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

[Close]

BLAST of MELO3C005272 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 1.6e-94
Identity = 177/182 (97.25%), Postives = 178/182 (97.80%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSH STDSPLGSTTLPI GSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120
           IMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKGVDS+EVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRS GF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

[Close]

BLAST of MELO3C005272 vs. NCBI nr
Match: gi|470125024|ref|XP_004298507.1| (PREDICTED: probable calcium-binding protein CML25 [Fragaria vesca subsp. vesca])

HSP 1 Score: 266.9 bits (681), Expect = 2.6e-68
Identity = 134/176 (76.14%), Postives = 153/176 (86.93%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKS PS+ +       P F +R QIAELEQVFNKFDVNGDGKI SSELG+
Sbjct: 1   MGFKSLFSRKKKSTPSSAAAPLVMRSPSFATRAQIAELEQVFNKFDVNGDGKISSSELGA 60

Query: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120
           IMGSLG PATE+EL +MI EVDADGDG+ID++EF+ELNTKG+DS E L+NL+DAFSVYD 
Sbjct: 61  IMGSLGHPATEDELRSMILEVDADGDGFIDINEFVELNTKGIDSAEALQNLRDAFSVYDK 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           DGNGSI+AEEL EVLKSLGD+CSLA+CRKMI GVD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 DGNGSISAEELFEVLKSLGDECSLAECRKMIGGVDADGDGMINFEEFKVMMMIGSR 176

[Close]

BLAST of MELO3C005272 vs. NCBI nr
Match: gi|703130743|ref|XP_010104706.1| (putative calcium-binding protein CML25 [Morus notabilis])

HSP 1 Score: 259.2 bits (661), Expect = 5.3e-66
Identity = 135/183 (73.77%), Postives = 153/183 (83.61%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRT-------QIAELEQVFNKFDVNGDGKI 60
           MGFKSLF R+KK   ST +P  S     F SR+       QI ELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKD 120
            SSELG+IMGSLGQP+ EEEL  MIKEVD+DGDG+IDL EFIELNTKGVDS+EVLENLKD
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 177
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+A+CRKMI+GVD +GDG ISF+EFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

[Close]

BLAST of MELO3C005272 vs. NCBI nr
Match: gi|596192889|ref|XP_007223528.1| (hypothetical protein PRUPE_ppa012032mg [Prunus persica])

HSP 1 Score: 258.8 bits (660), Expect = 7.0e-66
Identity = 133/179 (74.30%), Postives = 152/179 (84.92%), Query Frame = 1


Query: 1   MGFKSLFSRKKKSHPS-TDSPLGSTTL--PIFGSRTQIAELEQVFNKFDVNGDGKICSSE 60
           MGFKSLFSRKKKS PS T  PL +T    P F SR QIAEL+QVF KFDVNGDGKI SSE
Sbjct: 1   MGFKSLFSRKKKSIPSATALPLTTTPSRSPSFSSRAQIAELDQVFKKFDVNGDGKISSSE 60

Query: 61  LGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSV 120
           LG+IM SLGQPA EEEL +MI EVD+DGDG+ID  EF+ELNT GVD  E LENLKDAFSV
Sbjct: 61  LGAIMSSLGQPANEEELSHMILEVDSDGDGFIDFKEFVELNTNGVDQAEALENLKDAFSV 120

Query: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           YDIDGNGSI+AEEL++VL+SLGD+CS+A+C+KMI+ VD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 YDIDGNGSISAEELYKVLRSLGDECSIAECKKMISAVDSDGDGMINFEEFKVMMMMGSR 179

The following BLAST results are available for this feature:

BLAST of MELO3C005272 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML25_ARATH	2.9e-56	70.20	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML10_ORYSJ	2.1e-38	57.55	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ...	[more]
CML24_ARATH	3.1e-34	48.59	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
POLC2_JUNOX	3.1e-34	51.80	Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2	[more]
CML23_ARATH	2.0e-33	49.28	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]

BLAST of MELO3C005272 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KST2_CUCSA	1.1e-94	97.25	Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1	[more]
W9SF34_9ROSA	3.7e-66	73.77	Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV...	[more]
M5XF25_PRUPE	4.8e-66	74.30	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012032mg PE=4 SV=1	[more]
A0A0D2R7V3_GOSRA	1.9e-62	68.48	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G172400 PE=4 SV=1	[more]
K7KRI8_SOYBN	2.5e-62	73.45	Uncharacterized protein OS=Glycine max GN=GLYMA_05G238400 PE=4 SV=1	[more]

BLAST of MELO3C005272 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	1.6e-57	70.20	EF hand calcium-binding protein family	[more]
AT5G37770.1	1.8e-35	48.59	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.1e-34	49.28	calmodulin like 23	[more]
AT1G18210.1	2.4e-32	44.79	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	3.8e-30	41.04	EF hand calcium-binding protein family	[more]

BLAST of MELO3C005272 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659072787\|ref\|XP_008467096.1\|	1.4e-98	100.00	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]
gi\|778703916\|ref\|XP_011655449.1\|	1.6e-94	97.25	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus]	[more]
gi\|470125024\|ref\|XP_004298507.1\|	2.6e-68	76.14	PREDICTED: probable calcium-binding protein CML25 [Fragaria vesca subsp. vesca]	[more]
gi\|703130743\|ref\|XP_010104706.1\|	5.3e-66	73.77	putative calcium-binding protein CML25 [Morus notabilis]	[more]
gi\|596192889\|ref\|XP_007223528.1\|	7.0e-66	74.30	hypothetical protein PRUPE_ppa012032mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048868	pollen tube development
biological_process	GO:0048767	root hair elongation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0044424	intracellular part
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU54899	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C005272T1	MELO3C005272T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU54899	MU54899	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 109..171 score: 3.7E-12coord: 37..96 score: 4.2
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 73..101 score: 1.2E-4coord: 37..65 score: 7.9E-6coord: 146..174 score: 2.1E-5coord: 110..138 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 142..177 score: 12.505coord: 106..141 score: 15.434coord: 69..104 score: 13.593coord: 33..68 score: 14
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 32..174 score: 3.1
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 30..173 score: 4.7
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 119..131 score: -coord: 82..94 score: -coord: 155..167 score: -coord: 46..58 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 37..176 score: 6.6
None	No IPR available	PANTHER	PTHR10891:SF631	SUBFAMILY NOT NAMED	coord: 37..176 score: 6.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C005272	Csa5G561760	Cucumber (Chinese Long) v2	cumeB346
MELO3C005272	Cla003955	Watermelon (97103) v1	mewmB030
MELO3C005272	ClCG07G005670	Watermelon (Charleston Gray)	mewcgB033
MELO3C005272	ClCG01G006550	Watermelon (Charleston Gray)	mewcgB019
MELO3C005272	Cla97C07G133920	Watermelon (97103) v2	mewmbB032
MELO3C005272	Cla97C01G006860	Watermelon (97103) v2	mewmbB009
MELO3C005272	Bhi12G000820	Wax gourd	mewgoB012
MELO3C005272	CSPI05G19230	Wild cucumber (PI 183967)	cpimeB342
MELO3C005272	Cucsa.012210	Cucumber (Gy14) v1	cgymeB337
MELO3C005272	CmaCh15G009790	Cucurbita maxima (Rimu)	cmameB258
MELO3C005272	CmaCh04G021050	Cucurbita maxima (Rimu)	cmameB624
MELO3C005272	CmaCh04G000750	Cucurbita maxima (Rimu)	cmameB639
MELO3C005272	CmoCh04G022070	Cucurbita moschata (Rifu)	cmomeB614
MELO3C005272	CmoCh15G010260	Cucurbita moschata (Rifu)	cmomeB245
MELO3C005272	CmoCh04G000710	Cucurbita moschata (Rifu)	cmomeB629
MELO3C005272	Lsi09G006880	Bottle gourd (USVL1VR-Ls)	lsimeB013
MELO3C005272	Cp4.1LG01g06190	Cucurbita pepo (Zucchini)	cpemeB361
MELO3C005272	Cp4.1LG13g03490	Cucurbita pepo (Zucchini)	cpemeB149
MELO3C005272	Cp4.1LG01g18230	Cucurbita pepo (Zucchini)	cpemeB347
MELO3C005272	CsGy5G018760	Cucumber (Gy14) v2	cgybmeB305
MELO3C005272	Carg26054	Silver-seed gourd	carmeB0435
MELO3C005272	Carg23730	Silver-seed gourd	carmeB0933
MELO3C005272	CsaV3_5G028160	Cucumber (Chinese Long) v3	cucmeB363

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C005272	Cucurbita maxima (Rimu)	cmameB252
MELO3C005272	Cucurbita moschata (Rifu)	cmomeB240
MELO3C005272	Cucumber (Chinese Long) v2	cumeB355
MELO3C005272	Cucumber (Chinese Long) v2	cumeB364
MELO3C005272	Cucurbita pepo (Zucchini)	cpemeB157
MELO3C005272	Cucumber (Gy14) v2	cgybmeB293
MELO3C005272	Silver-seed gourd	carmeB0869
MELO3C005272	Cucumber (Chinese Long) v3	cucmeB350
MELO3C005272	Cucumber (Chinese Long) v3	cucmeB355