Cucsa.012210 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.012210
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPolcalcin Jun o
Locationscaffold00154 : 1686534 .. 1687082 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATTCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTCTCGGATCCCGTACCCAAATCGCTGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAGCTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCAGAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGAGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACTGGCGTTGATAAAAACGGCGATGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCAAGGGTTTAATGGCTAA

mRNA sequence

ATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATTCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTCTCGGATCCCGTACCCAAATCGCTGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAGCTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCAGAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGAGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACTGGCGTTGATAAAAACGGCGATGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCAAGGGTTTAATGGCTAA

Coding sequence (CDS)

ATGGGTTTCAAATCTCTCTTCTCCCGTAAGAAGAAATCCCATTCCTCCACCGATTCTCCCCTTGGATCTACCACCCTTCCCATTCTCGGATCCCGTACCCAAATCGCTGAACTCGAGCAAGTTTTCAACAAATTCGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAGCTCGGATCTATTATGGGTAGCCTAGGTCAGCCCGCCACCGAGGAAGAGCTTCAGAACATGATTAAGGAGGTCGATGCCGACGGCGATGGCTACATTGATCTTGATGAGTTCATCGAACTCAACACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCCTTCTCTGTTTACGATATTGACGGAAACGGATCCATCACTGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGAGATGATTGCTCCTTGGCGGATTGCCGGAAGATGATTACTGGCGTTGATAAAAACGGCGATGGTATGATTAGTTTCGATGAATTTAAAGTGATGATGATGAGCGGTTCTCGATCGCAAGGGTTTAATGGCTAA

Protein sequence

MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGFNG*
Homology
[Close]
BLAST of Cucsa.012210 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.3e-56
Identity = 111/182 (60.99%), Postives = 137/182 (75.27%), Query Frame = 1 

Query: 6   LFSRKKKSHSSTDSPLG-STTLPIL----GSRTQIAELEQVFNKFDVNGDGKICSSELGS 65
           +F++ + S+  + S +G     P L      +T+I ELE VF KFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D ++VLENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 183
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVDK+GDG I F+EFK+MM  GSR    
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

[Close]
BLAST of Cucsa.012210 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.6e-38
Identity = 80/139 (57.55%), Postives = 102/139 (73.38%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           E+E+VF KFD NGDG+I  SELG++  SLG  AT++EL  M+ E DADGDG+I LDEF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 97  LN-TKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD 156
           LN T   D+  V E+L+ AF V+D DGNG+I+A EL  VL  LG+  ++  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 157 KNGDGMISFDEFKVMMMSG 175
           +NGDG+ISF+EFKVMM  G
Sbjct: 160 QNGDGLISFEEFKVMMAGG 178

[Close]
BLAST of Cucsa.012210 vs. Swiss-Prot
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-34
Identity = 72/139 (51.80%), Postives = 99/139 (71.22%), Query Frame = 1 

Query: 35  IAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEF 94
           + ELE+VF KFD NGDGKI  SEL  I+ SLG    E E++ M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 95  IELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 154
           ++LN KG      +++LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 155 DKNGDGMISFDEFKVMMMS 174
           DKNGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

[Close]
BLAST of Cucsa.012210 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 4.1e-34
Identity = 69/142 (48.59%), Postives = 103/142 (72.54%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 97  LNTKGV----DSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 156
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 157 GVDKNGDGMISFDEFKVMMMSG 175
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of Cucsa.012210 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.0e-33
Identity = 68/140 (48.57%), Postives = 99/140 (70.71%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           E E+VF KFD NGDG+I  +EL ++  S+G   T++E+  M++E D+DGDGYI L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 97  LNTKGV-DSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD 156
           ++     D+    E+L+ AF V+D DGNG IT  EL  VL+ +G+  ++A CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 157 KNGDGMISFDEFKVMMMSGS 176
           +NGDG+I+F+EFK+MM +G+
Sbjct: 175 RNGDGLINFEEFKLMMAAGA 194

[Close]
BLAST of Cucsa.012210 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 1.8e-97
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

[Close]
BLAST of Cucsa.012210 vs. TrEMBL
Match: W9SF34_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.2e-67
Identity = 136/183 (74.32%), Postives = 155/183 (84.70%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGS-------TTLPILGSRTQIAELEQVFNKFDVNGDGKI 60
           MGFKSLF R+KK  SST +P  S       +  P +  R QI ELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120
            SSELG+IMGSLGQP+ EEEL+ MIKEVD+DGDG+IDL EFIELNTKGVDSDEVLENLKD
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 177
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+A+CRKMI+GVD +GDG ISF+EFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

[Close]
BLAST of Cucsa.012210 vs. TrEMBL
Match: W9SF34_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.8e-03
Identity = 25/79 (31.65%), Postives = 42/79 (53.16%), Query Frame = 1 

Query: 35  IAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEF 94
           +  L+  F+ +D++ +G I + EL  ++ SLG   +  E + MI  VD DGDG I  +EF
Sbjct: 115 LENLKDAFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEF 174

Query: 95  IELNTKGVDSDEVLENLKD 114
             +   G   D + +  K+
Sbjct: 175 KVMMMTGSRYDALSQRSKN 193


HSP 2 Score: 255.0 bits (650), Expect = 7.0e-65
Identity = 131/179 (73.18%), Postives = 151/179 (84.36%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKS-HSSTDSPLGSTTL--PILGSRTQIAELEQVFNKFDVNGDGKICSSE 60
           MGFKSLFSRKKKS  S+T  PL +T    P   SR QIAEL+QVF KFDVNGDGKI SSE
Sbjct: 1   MGFKSLFSRKKKSIPSATALPLTTTPSRSPSFSSRAQIAELDQVFKKFDVNGDGKISSSE 60

Query: 61  LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSV 120
           LG+IM SLGQPA EEEL +MI EVD+DGDG+ID  EF+ELNT GVD  E LENLKDAFSV
Sbjct: 61  LGAIMSSLGQPANEEELSHMILEVDSDGDGFIDFKEFVELNTNGVDQAEALENLKDAFSV 120

Query: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           YDIDGNGSI+AEEL++VL+SLGD+CS+A+C+KMI+ VD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 YDIDGNGSISAEELYKVLRSLGDECSIAECKKMISAVDSDGDGMINFEEFKVMMMMGSR 179

[Close]
BLAST of Cucsa.012210 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 5.9e-64
Identity = 131/193 (67.88%), Postives = 153/193 (79.27%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSP--LGSTTLPILGS---------------RTQIAELEQVFN 60
           MGF S+F+RKKK HS +++    GS  +   GS               +TQ+ ELE+VF 
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVD 120
           KFDVNGDGKI SSELGSIMGSLG   +E+ELQ MIKE DADGDG I+  EF+ELNTKGVD
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 SDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMIS 177
           SDEVLENLKDAFSVYDIDGNGSI+AEELHEVLKSLGD+CS+A+CRKMI+GVD +GDGMI 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]
BLAST of Cucsa.012210 vs. TrEMBL
Match: K7KRI8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G238400 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.2e-63
Identity = 132/177 (74.58%), Postives = 153/177 (86.44%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIA-ELEQVFNKFDVNGDGKICSSELG 60
           MG KSLF+R K + SS D+   ST+L +  SRT++A ELEQVFNKFDVNGDGKI +SELG
Sbjct: 1   MGLKSLFNRNKDAISS-DTASRSTSLSVR-SRTRMADELEQVFNKFDVNGDGKISASELG 60

Query: 61  SIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYD 120
           SIMGSLGQPATE EL NMI+EVD DGDG I L EFIELNTKGVDSDEVLENLKDAF+V+D
Sbjct: 61  SIMGSLGQPATELELDNMIREVDGDGDGCISLPEFIELNTKGVDSDEVLENLKDAFAVFD 120

Query: 121 IDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           IDGNGSITAEEL+ V++SLG+DCSLA+CR+MI+GVD +GDG I F+EF+VMMM GSR
Sbjct: 121 IDGNGSITAEELNTVMRSLGEDCSLAECRRMISGVDGDGDGTIDFEEFRVMMMMGSR 175

[Close]
BLAST of Cucsa.012210 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 220.7 bits (561), Expect = 7.4e-58
Identity = 111/182 (60.99%), Postives = 137/182 (75.27%), Query Frame = 1 

Query: 6   LFSRKKKSHSSTDSPLG-STTLPIL----GSRTQIAELEQVFNKFDVNGDGKICSSELGS 65
           +F++ + S+  + S +G     P L      +T+I ELE VF KFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D ++VLENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 183
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVDK+GDG I F+EFK+MM  GSR    
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

[Close]
BLAST of Cucsa.012210 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 146.0 bits (367), Expect = 2.3e-35
Identity = 69/142 (48.59%), Postives = 103/142 (72.54%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 97  LNTKGV----DSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 156
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 157 GVDKNGDGMISFDEFKVMMMSG 175
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of Cucsa.012210 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 142.5 bits (358), Expect = 2.6e-34
Identity = 67/138 (48.55%), Postives = 98/138 (71.01%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           ++++VF +FD N DGKI   EL  ++G+L   A++EE + M+KE D DG+G+IDLDEF+ 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 97  LNTKGVDS--DEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 156
           L      S  +  + +LK+AF +YD+D NG I+A ELH V+K+LG+ CS+ DC++MI  V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 157 DKNGDGMISFDEFKVMMM 173
           D +GDG + F+EFK MMM
Sbjct: 135 DSDGDGCVDFEEFKKMMM 152

[Close]
BLAST of Cucsa.012210 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 135.2 bits (339), Expect = 4.1e-32
Identity = 69/139 (49.64%), Postives = 93/139 (66.91%), Query Frame = 1 

Query: 37  ELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 96
           EL++VF++FD NGDGKI   ELG +  ++G   TE EL  +++EVD D DGYI+LDEF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 97  LNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDK 156
           L      + E+    +DAF +YD D NG I+A ELH+VL  LG  CS+ DC +MI  VD 
Sbjct: 83  LCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 157 NGDGMISFDEFKVMMMSGS 176
           +GDG ++F+EF+ MM S S
Sbjct: 143 DGDGNVNFEEFQKMMTSSS 157

[Close]
BLAST of Cucsa.012210 vs. TAIR10
Match: AT5G21274.1 (AT5G21274.1 calmodulin 6)

HSP 1 Score: 127.5 bits (319), Expect = 8.5e-30
Identity = 66/140 (47.14%), Postives = 94/140 (67.14%), Query Frame = 1 

Query: 34  QIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDE 93
           QI+E ++ F+ FD +GDG I + ELG++M SLGQ  TE ELQ+MI EVDADG+G ID  E
Sbjct: 9   QISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPE 68

Query: 94  FIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITG 153
           F+ L  + +   +  E LK+AF V+D D NG I+A EL  V+ +LG+  S  +  +MI  
Sbjct: 69  FLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDEMIRE 128

Query: 154 VDKNGDGMISFDEFKVMMMS 174
            D +GDG I+++EF  +MM+
Sbjct: 129 ADVDGDGQINYEEFVKVMMA 148

[Close]
BLAST of Cucsa.012210 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 363.2 bits (931), Expect = 2.6e-97
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

[Close]
BLAST of Cucsa.012210 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 354.0 bits (907), Expect = 1.6e-94
Identity = 177/182 (97.25%), Postives = 178/182 (97.80%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60
           MGFKSLFSRKKKSH STDSPLGSTTLPI GSRTQIAELEQVFNKFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKGVDS+EVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRS GF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180

Query: 181 NG 183
           NG
Sbjct: 181 NG 182

[Close]
BLAST of Cucsa.012210 vs. NCBI nr
Match: gi|703130743|ref|XP_010104706.1| (putative calcium-binding protein CML25 [Morus notabilis])

HSP 1 Score: 264.2 bits (674), Expect = 1.7e-67
Identity = 136/183 (74.32%), Postives = 155/183 (84.70%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKSHSSTDSPLGS-------TTLPILGSRTQIAELEQVFNKFDVNGDGKI 60
           MGFKSLF R+KK  SST +P  S       +  P +  R QI ELEQVF KFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120
            SSELG+IMGSLGQP+ EEEL+ MIKEVD+DGDG+IDL EFIELNTKGVDSDEVLENLKD
Sbjct: 61  SSSELGAIMGSLGQPSGEEELRKMIKEVDSDGDGFIDLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 177
           AFSVYDID NGSI+AEEL+EVL+SLGD+CS+A+CRKMI+GVD +GDG ISF+EFKVMMM+
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

[Close]
BLAST of Cucsa.012210 vs. NCBI nr
Match: gi|703130743|ref|XP_010104706.1| (putative calcium-binding protein CML25 [Morus notabilis])

HSP 1 Score: 49.3 bits (116), Expect = 8.4e-03
Identity = 25/79 (31.65%), Postives = 42/79 (53.16%), Query Frame = 1 

Query: 35  IAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEF 94
           +  L+  F+ +D++ +G I + EL  ++ SLG   +  E + MI  VD DGDG I  +EF
Sbjct: 115 LENLKDAFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEF 174

Query: 95  IELNTKGVDSDEVLENLKD 114
             +   G   D + +  K+
Sbjct: 175 KVMMMTGSRYDALSQRSKN 193


HSP 2 Score: 262.3 bits (669), Expect = 6.3e-67
Identity = 135/177 (76.27%), Postives = 155/177 (87.57%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKS-HSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELG 60
           MGFKSLFSRKKKS  SS  +PL   + P   +R QIAELEQVFNKFDVNGDGKI SSELG
Sbjct: 1   MGFKSLFSRKKKSTPSSAAAPLVMRS-PSFATRAQIAELEQVFNKFDVNGDGKISSSELG 60

Query: 61  SIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYD 120
           +IMGSLG PATE+EL++MI EVDADGDG+ID++EF+ELNTKG+DS E L+NL+DAFSVYD
Sbjct: 61  AIMGSLGHPATEDELRSMILEVDADGDGFIDINEFVELNTKGIDSAEALQNLRDAFSVYD 120

Query: 121 IDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
            DGNGSI+AEEL EVLKSLGD+CSLA+CRKMI GVD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 KDGNGSISAEELFEVLKSLGDECSLAECRKMIGGVDADGDGMINFEEFKVMMMIGSR 176

[Close]
BLAST of Cucsa.012210 vs. NCBI nr
Match: gi|596192889|ref|XP_007223528.1| (hypothetical protein PRUPE_ppa012032mg [Prunus persica])

HSP 1 Score: 255.0 bits (650), Expect = 1.0e-64
Identity = 131/179 (73.18%), Postives = 151/179 (84.36%), Query Frame = 1 

Query: 1   MGFKSLFSRKKKS-HSSTDSPLGSTTL--PILGSRTQIAELEQVFNKFDVNGDGKICSSE 60
           MGFKSLFSRKKKS  S+T  PL +T    P   SR QIAEL+QVF KFDVNGDGKI SSE
Sbjct: 1   MGFKSLFSRKKKSIPSATALPLTTTPSRSPSFSSRAQIAELDQVFKKFDVNGDGKISSSE 60

Query: 61  LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSV 120
           LG+IM SLGQPA EEEL +MI EVD+DGDG+ID  EF+ELNT GVD  E LENLKDAFSV
Sbjct: 61  LGAIMSSLGQPANEEELSHMILEVDSDGDGFIDFKEFVELNTNGVDQAEALENLKDAFSV 120

Query: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 177
           YDIDGNGSI+AEEL++VL+SLGD+CS+A+C+KMI+ VD +GDGMI+F+EFKVMMM GSR
Sbjct: 121 YDIDGNGSISAEELYKVLRSLGDECSIAECKKMISAVDSDGDGMINFEEFKVMMMMGSR 179

The following BLAST results are available for this feature:
BLAST of Cucsa.012210 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML25_ARATH1.3e-5660.99Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ1.6e-3857.55Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
POLC2_JUNOX1.8e-3451.80Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2[more]
CML24_ARATH4.1e-3448.59Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2[more]
CML15_ORYSJ2.0e-3348.57Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ... [more]
BLAST of Cucsa.012210 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KST2_CUCSA1.8e-97100.00Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1[more]
W9SF34_9ROSA1.2e-6774.32Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV... [more]
W9SF34_9ROSA5.8e-0331.65Putative calcium-binding protein CML25 OS=Morus notabilis GN=L484_000710 PE=4 SV... [more]
A0A061E9N9_THECC5.9e-6467.88Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1[more]
K7KRI8_SOYBN2.2e-6374.58Uncharacterized protein OS=Glycine max GN=GLYMA_05G238400 PE=4 SV=1[more]
BLAST of Cucsa.012210 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.17.4e-5860.99 EF hand calcium-binding protein family[more]
AT5G37770.12.3e-3548.59 EF hand calcium-binding protein family[more]
AT1G66400.12.6e-3448.55 calmodulin like 23[more]
AT1G18210.14.1e-3249.64 Calcium-binding EF-hand family protein[more]
AT5G21274.18.5e-3047.14 calmodulin 6[more]
BLAST of Cucsa.012210 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778703916|ref|XP_011655449.1|2.6e-97100.00PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|659072787|ref|XP_008467096.1|1.6e-9497.25PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|703130743|ref|XP_010104706.1|1.7e-6774.32putative calcium-binding protein CML25 [Morus notabilis][more]
gi|703130743|ref|XP_010104706.1|8.4e-0331.65putative calcium-binding protein CML25 [Morus notabilis][more]
gi|596192889|ref|XP_007223528.1|1.0e-6473.18hypothetical protein PRUPE_ppa012032mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048868 pollen tube development
biological_process GO:0048767 root hair elongation
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.012210.1Cucsa.012210.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 37..96
score: 4.1E-14coord: 109..171
score: 3.7
IPR002048EF-hand domainSMARTSM00054efh_1coord: 37..65
score: 7.9E-6coord: 146..174
score: 2.1E-5coord: 73..101
score: 4.2E-5coord: 110..138
score: 1.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 106..141
score: 15.434coord: 69..104
score: 14.095coord: 33..68
score: 14.848coord: 142..177
score: 12
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 32..175
score: 1.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 31..174
score: 9.88
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 46..58
score: -coord: 82..94
score: -coord: 155..167
score: -coord: 119..131
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 37..176
score: 6.1
NoneNo IPR availablePANTHERPTHR10891:SF631SUBFAMILY NOT NAMEDcoord: 37..176
score: 6.1