Cp4.1LG01g06190 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG01g06190
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPolcalcin Jun o
LocationCp4.1LG01 : 357745 .. 358305 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCTAAGATGATCGAGGTATTCGACGCGGATGGCGACGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGATAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAATGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGCTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

mRNA sequence

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCTAAGATGATCGAGGTATTCGACGCGGATGGCGACGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGATAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAATGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGCTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Coding sequence (CDS)

ATGAGATTCAAATGTCTCTTCTGCTGGAAAAAGAAGCCCTTCGCCTCCGTCACTTCTTCCATCGTCTCCTCGCCCTCGACGAATGGACCTCGCTCCTCATCCAGAGATTCACACTCTCGGAACGCGGAACTCGAGGAGGTTTTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCCGAATTAGGCTCGATTATGGGGAGCCTAGGGCACAAGGCCACGGAAGAAGAGCTCGCTAAGATGATCGAGGTATTCGACGCGGATGGCGACGGATTCATTAATCTGCAGGAATTTGTGGAGTTAAACACTAAAGAAATCGATCCCGAGGAGTTGTTGGATAATCTGAGAGAGGCATTTTCGGTTTACGATATCGATGGAAATGGATCGATCTCGGCGGAGGAACTGCATAAGGTTATGAGGAGCTTGGGGGACGATTGCTCGGTGGCAGATTGCCGGCAGATGATCAGCGGCGTCGATAGAAACGGCGACGGACTGATTAGCTTCGATGAGTTCAAGGTAATGATGTCGGCTGGGCTCTCCGTCGCAGGCGGTTGA

Protein sequence

MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAGLSVAGG
Homology
[Close]
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 2.9e-51
Identity = 98/147 (66.67%), Postives = 121/147 (82.31%), Query Frame = 1 

Query: 34  SRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDG 93
           +R   +   ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL K I   D  GDG
Sbjct: 28  ARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDG 87

Query: 94  FINLQEFVELNTKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADC 153
           +IN +EFVELNTK +D  ++L+NL++AFSVYDIDGNGSISAEELH+V+RSLGD+CS+A+C
Sbjct: 88  YINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAEC 147

Query: 154 RQMISGVDRNGDGLISFDEFKVMMSAG 181
           R+MI GVD++GDG I F+EFK+MM+ G
Sbjct: 148 RKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-40
Identity = 84/143 (58.74%), Postives = 108/143 (75.52%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E+E VF+KFD NGDG+IS +ELG++  SLGH AT++ELA+M+   DADGDGFI+L EF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 103 LN-TKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVD 162
           LN T   D   + ++LR AF V+D DGNG+ISA EL +V+  LG+  +V  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 163 RNGDGLISFDEFKVMMSAGLSVA 185
           +NGDGLISF+EFKVMM+ G S A
Sbjct: 160 QNGDGLISFEEFKVMMAGGGSFA 182

[Close]
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 73/139 (52.52%), Postives = 103/139 (74.10%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+A+M++  D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 103 LNTKEI-DPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVD 162
           ++     D     ++LR AF V+D DGNG I+  EL +V+R +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 163 RNGDGLISFDEFKVMMSAG 181
           RNGDGLI+F+EFK+MM+AG
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-33
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 GVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.3e-32
Identity = 73/151 (48.34%), Postives = 97/151 (64.24%), Query Frame = 1 

Query: 42  AELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFV 101
           AELE+VF+++D NGDGKIS+ EL S++ +LG      E+ +M++  D+D DGF++L EF 
Sbjct: 4   AELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFA 63

Query: 102 ELNTKEI-----------DPEELLD-NLREAFSVYDIDGNGSISAEELHKVMRSLGDDCS 161
             +               D E   +  LREAF +YD D NG ISA ELH+V+R LGD CS
Sbjct: 64  AFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCS 123

Query: 162 VADCRQMISGVDRNGDGLISFDEFKVMMSAG 181
           VADC +MI  VD +GDG ++FDEFK MM  G
Sbjct: 124 VADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 2.6e-75
Identity = 154/185 (83.24%), Postives = 164/185 (88.65%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELL+NLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-56
Identity = 120/180 (66.67%), Postives = 146/180 (81.11%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   SR   ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGSR---TQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+L+NL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVMM +G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.9e-56
Identity = 121/191 (63.35%), Postives = 147/191 (76.96%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFA-SVTSSIVSSPSTNGPRSSSRDSHSRNA----------ELEEVFK 60
           M F  +F  KKK  + S  S++  SP+     SS   S  R            ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEID 120
           KFDVNGDGKISS+ELGSIMGSLGHK +E+EL KMI+ FDADGDG IN +EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLIS 180
            +E+L+NL++AFSVYDIDGNGSISAEELH+V++SLGD+CS+A+CR+MISGVD +GDG+I 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match: A0A151S400_CAJCA (Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.8e-55
Identity = 118/187 (63.10%), Postives = 145/187 (77.54%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNG-PRSSSRDSHSRNAE-LEEVFKKFDVNGDGK 60
           M F+ LFC K+K   + +SS  S+ ST+  P +       R AE LE VFKKFDVNGDGK
Sbjct: 1   MGFRSLFCRKRKMLKTTSSSSASTSSTSALPTAIPGSGRGRVAEELEYVFKKFDVNGDGK 60

Query: 61  ISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLR 120
           ISS+ELGS+M SLG  ATEEEL+KMI   DADGDG I+L+EF+ELNTK +DP+E+L+NLR
Sbjct: 61  ISSSELGSMMRSLGQAATEEELSKMIGEVDADGDGHISLEEFIELNTKGVDPDEVLENLR 120

Query: 121 EAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMS 180
           +AFS++DIDGNGSI+ EELH VM SLGD CSV DCR+MI+GVD +GDG I FDEFK+MM+
Sbjct: 121 DAFSIFDIDGNGSITVEELHMVMASLGDQCSVDDCRKMIAGVDADGDGTIDFDEFKIMMT 180

Query: 181 AGLSVAG 186
           A  S +G
Sbjct: 181 ASASASG 187

[Close]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match: A0A067KPP2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-54
Identity = 118/184 (64.13%), Postives = 139/184 (75.54%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSR----NAELEEVFKKFDVNGD 60
           M FK LF  KK   +S +S +  + S   P S S     R      EL+ VF KFD NGD
Sbjct: 1   MGFKALFNRKKSKKSSDSSVVSPAGSPPDPLSRSGSQSLRLKHQTDELQHVFNKFDANGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDN 120
           GKISSAELGSIM SLGHKAT+EEL KMI  FDADGDGFI+ QEFVELNT+ +D  E ++N
Sbjct: 61  GKISSAELGSIMASLGHKATDEELRKMIMEFDADGDGFIDFQEFVELNTQGVDTNEAMEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVM 180
           LR+AFSVYDIDGNGSISAEELH+VM SLG+ CS+A+CR+MISGVD +GDG+I F+EFKVM
Sbjct: 121 LRDAFSVYDIDGNGSISAEELHRVMGSLGESCSIAECRRMISGVDGDGDGMIDFEEFKVM 180

[Close]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 203.0 bits (515), Expect = 1.6e-52
Identity = 98/147 (66.67%), Postives = 121/147 (82.31%), Query Frame = 1 

Query: 34  SRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDG 93
           +R   +   ELE VFKKFDVNGDGKISS ELG+IM SLGH+  EEEL K I   D  GDG
Sbjct: 28  ARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDG 87

Query: 94  FINLQEFVELNTKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADC 153
           +IN +EFVELNTK +D  ++L+NL++AFSVYDIDGNGSISAEELH+V+RSLGD+CS+A+C
Sbjct: 88  YINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAEC 147

Query: 154 RQMISGVDRNGDGLISFDEFKVMMSAG 181
           R+MI GVD++GDG I F+EFK+MM+ G
Sbjct: 148 RKMIGGVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 142.1 bits (357), Expect = 3.4e-34
Identity = 73/142 (51.41%), Postives = 102/142 (71.83%), Query Frame = 1 

Query: 43  ELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVE 102
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M++ FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 103 LNTKEI----DPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMIS 162
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH VM++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 163 GVDRNGDGLISFDEFKVMMSAG 181
            VD +GDG ++FDEFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 135.6 bits (340), Expect = 3.2e-32
Identity = 68/149 (45.64%), Postives = 103/149 (69.13%), Query Frame = 1 

Query: 31  RSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDAD 90
           ++ SR+      ++++VF++FD N DGKIS  EL  ++G+L   A++EE   M++ FD D
Sbjct: 3   KNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLD 62

Query: 91  GDGFINLQEFVELN--TKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDC 150
           G+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH VM++LG+ C
Sbjct: 63  GNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKC 122

Query: 151 SVADCRQMISGVDRNGDGLISFDEFKVMM 178
           S+ DC++MI+ VD +GDG + F+EFK MM
Sbjct: 123 SIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 132.9 bits (333), Expect = 2.1e-31
Identity = 73/160 (45.62%), Postives = 103/160 (64.38%), Query Frame = 1 

Query: 26  STNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIE 85
           +TN   ++   + S + EL++VF KFD NGDGKIS +ELG++  S+G   TEEEL ++++
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 86  VFDADGDGFINLQEFVELNTKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLG 145
             D D DGFIN +EF  +        E+    REAF +YD + NG IS+ E+HKV+  LG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 146 DDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAGLSVAG 186
             CSV DC +MI  VD +GDG ++F+EF+ MMS+   V G
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMSSPELVKG 158

[Close]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 1 

Query: 23  SSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKISSAELGSIMGSLGHKATEEELAK 82
           ++P T  P  ++ D  +   EL++VF +FD NGDGKIS  ELG +  ++G   TE EL +
Sbjct: 4   ANPETAKPTPATVDM-ANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 63

Query: 83  MIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREAFSVYDIDGNGSISAEELHKVMR 142
           ++E  D D DG+INL EF  L        E+    R+AF +YD D NG ISA ELH+V+ 
Sbjct: 64  VLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLN 123

Query: 143 SLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSA 180
            LG  CSV DC +MI  VD +GDG ++F+EF+ MM++
Sbjct: 124 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTS 155

[Close]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 293.1 bits (749), Expect = 3.4e-76
Identity = 157/186 (84.41%), Postives = 165/186 (88.71%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS   S S+ AELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120
           SAELGSIM SLGH ATEEEL KMIE FDADGDGFINL EFVELNTKEIDPEELL+NLREA
Sbjct: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV+RSLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAGG 187
           LSVAGG
Sbjct: 181 LSVAGG 185

[Close]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 289.7 bits (740), Expect = 3.7e-75
Identity = 154/185 (83.24%), Postives = 164/185 (88.65%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFRSLFR-KKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120
           SAELGSIM SLGH ATEEEL KMI+ FDADGDGFINL EFVELNTKEIDPEELL+NLREA
Sbjct: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSISAEELHKV++SLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180

Query: 181 LSVAG 186
           LSVAG
Sbjct: 181 LSVAG 184

[Close]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-56
Identity = 120/180 (66.67%), Postives = 146/180 (81.11%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60
           M FK LF  KKK  +S  S +    ST  P   SR   ++ AELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPL---GSTTLPILGSR---TQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDNLREA 120
           S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D +E+L+NL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180
           FSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVMM +G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match: gi|659072787|ref|XP_008467096.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 3.0e-56
Identity = 120/184 (65.22%), Postives = 145/184 (78.80%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSR----DSHSRNAELEEVFKKFDVNGD 60
           M FK LF  KKK          S PST+ P  S+      S ++ AELE+VF KFDVNGD
Sbjct: 1   MGFKSLFSRKKK----------SHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGD 60

Query: 61  GKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEIDPEELLDN 120
           GKI S+ELGSIMGSLG  ATEEEL  MI+  DADGDG+I+L EF+ELNTK +D  E+L+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120

Query: 121 LREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVM 180
           L++AFSVYDIDGNGSI+AEELH+V++SLGDDCS+ADCR+MI+GVD+NGDG+ISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVM 174

[Close]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match: gi|590696790|ref|XP_007045260.1| (Polcalcin Jun o, putative [Theobroma cacao])

HSP 1 Score: 224.9 bits (572), Expect = 1.1e-55
Identity = 121/191 (63.35%), Postives = 147/191 (76.96%), Query Frame = 1 

Query: 1   MRFKCLFCWKKKPFA-SVTSSIVSSPSTNGPRSSSRDSHSRNA----------ELEEVFK 60
           M F  +F  KKK  + S  S++  SP+     SS   S  R            ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSAELGSIMGSLGHKATEEELAKMIEVFDADGDGFINLQEFVELNTKEID 120
           KFDVNGDGKISS+ELGSIMGSLGHK +E+EL KMI+ FDADGDG IN +EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLDNLREAFSVYDIDGNGSISAEELHKVMRSLGDDCSVADCRQMISGVDRNGDGLIS 180
            +E+L+NL++AFSVYDIDGNGSISAEELH+V++SLGD+CS+A+CR+MISGVD +GDG+I 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g06190 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML25_ARATH2.9e-5166.67Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ3.0e-4058.74Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
CML15_ORYSJ1.3e-3552.52Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ... [more]
CML24_ARATH6.0e-3351.41Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2[more]
CML18_ORYSJ2.3e-3248.34Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of Cp4.1LG01g06190 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KX11_CUCSA2.6e-7583.24Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1[more]
A0A0A0KST2_CUCSA1.2e-5666.67Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1[more]
A0A061E9N9_THECC7.9e-5663.35Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1[more]
A0A151S400_CAJCA1.8e-5563.10Putative calcium-binding protein CML25 OS=Cajanus cajan GN=KK1_028776 PE=4 SV=1[more]
A0A067KPP2_JATCU2.5e-5464.13Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04755 PE=4 SV=1[more]
BLAST of Cp4.1LG01g06190 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.11.6e-5266.67 EF hand calcium-binding protein family[more]
AT5G37770.13.4e-3451.41 EF hand calcium-binding protein family[more]
AT1G66400.13.2e-3245.64 calmodulin like 23[more]
AT1G73630.12.1e-3145.63 EF hand calcium-binding protein family[more]
AT1G18210.11.1e-2945.22 Calcium-binding EF-hand family protein[more]
BLAST of Cp4.1LG01g06190 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659129528|ref|XP_008464718.1|3.4e-7684.41PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|449449242|ref|XP_004142374.1|3.7e-7583.24PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|778703916|ref|XP_011655449.1|1.7e-5666.67PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|659072787|ref|XP_008467096.1|3.0e-5665.22PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|590696790|ref|XP_007045260.1|1.1e-5563.35Polcalcin Jun o, putative [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0048767 root hair elongation
biological_process GO:0009409 response to cold
cellular_component GO:0044424 intracellular part
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g06190.1Cp4.1LG01g06190.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 43..103
score: 7.8E-13coord: 116..177
score: 2.3
IPR002048EF-hand domainSMARTSM00054efh_1coord: 152..180
score: 1.8E-5coord: 79..107
score: 7.2E-5coord: 43..71
score: 4.7E-7coord: 116..144
score: 1.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 39..74
score: 15.797coord: 75..110
score: 13.342coord: 148..183
score: 12.365coord: 112..147
score: 15
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 37..178
score: 1.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 40..178
score: 2.67
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 52..64
score: -coord: 161..173
score: -coord: 88..100
score: -coord: 125..137
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 43..180
score: 3.4
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 43..180
score: 3.4