CSPI05G27880 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI05G27880
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPolcalcin Jun o
LocationChr5 : 26341890 .. 26342995 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATATATATAGAAAGCTAGAGTTTTGTGTTGCCAACTCATCCTCTTCTACGATAATTTTTTATTCTCAAGTGAAAGATGCATCTTATCATATGATGATACATTCTCCCACAATATATAGAAAAAAATAAAATAAAATCTCAACCTCTGTTAGTGCCATTCCACCATTTGTTAATCTTTCTCTCTCTCGTCCATTTCTCTATAATTGGATTGGATTGGTATTCAACAAACTGTAGCCAATCTCAAATCCCCATTTTCCTTCCCCGCTTCAATTCTCCCCTAAATCGATAATGCTCAATTTCTCCTTCCTGTTCATCCTCAATCCTCCGATTTGCATTTCTTTCTGATATCGGTTTTGGTGGTAATTAATTCATCGGCTGTTCTTCGCAGCAGAGAGAAATAATTCGACCATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGAGTTTGAACATCCGCTGGTAGATTTCGAGAGGAATTTGCTAGAATTCGGATTTTTTTTTCTTCTTCCCTTCTGTCTCCGACGAAAGTACTGGAAGTTCTTCACCGTTATATATATATATTATTTGTAGAATTGTAAACAAC

mRNA sequence

ATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

Coding sequence (CDS)

ATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA
Homology
[Close]
BLAST of CSPI05G27880 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-52
Identity = 101/143 (70.63%), Postives = 120/143 (83.92%), Query Frame = 1 

Query: 37  ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IM SLGH   EEEL K I E D  GDG+IN 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of CSPI05G27880 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-40
Identity = 95/180 (52.78%), Postives = 121/180 (67.22%), Query Frame = 1 

Query: 5   SLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISSAELG 64
           +LFR++    S + PL  +    G  S     E    E+E VF+KFD NGDG+IS +ELG
Sbjct: 8   ALFRRRS--GSKSPPLPQADPASGGGSPAPTPEE---EMERVFRKFDANGDGRISRSELG 67

Query: 65  SIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELN-TKEIDPEELLENLREAFSVY 124
           ++  SLGH AT++EL +M+ E DADGDGFI+L EF  LN T   D   + E+LR AF V+
Sbjct: 68  ALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVF 127

Query: 125 DIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVA 184
           D DGNG+ISA EL +VL  LG+  +V  CR+MI GVD NGDG+ISFEEFKVMM+ G S A
Sbjct: 128 DADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGSFA 182

[Close]
BLAST of CSPI05G27880 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.8e-35
Identity = 73/139 (52.52%), Postives = 101/139 (72.66%), Query Frame = 1 

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 101
           E E VF+KFD NGDG+IS AEL ++ RS+GH  T++E+ +M+ E D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 102 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVD 161
           ++     D     E+LR AF V+D DGNG I+  EL +VL+ +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 162 SNGDGMISFEEFKVMMSTG 180
            NGDG+I+FEEFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]
BLAST of CSPI05G27880 vs. Swiss-Prot
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 3.2e-34
Identity = 78/160 (48.75%), Postives = 111/160 (69.38%), Query Frame = 1 

Query: 25  STLGSRSSLDDS---ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGK 84
           S+ GS+S+       E  + ELEEVFKKFD NGDGKIS +EL  I+RSLG +  E E+  
Sbjct: 6   SSDGSKSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKA 65

Query: 85  MIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQ 144
           M++E DADGDG+++L EFV+LN K       +++L+ AF V+D D NGSISA EL   L+
Sbjct: 66  MMEEADADGDGYVSLQEFVDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLE 125

Query: 145 SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLS 182
           S+G+ C++ + + +I  VD NGDG+IS EEF+ MM++ ++
Sbjct: 126 SVGEPCTIEESKNIIHNVDKNGDGLISVEEFQTMMTSEMT 161

[Close]
BLAST of CSPI05G27880 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 4.6e-33
Identity = 75/152 (49.34%), Postives = 100/152 (65.79%), Query Frame = 1 

Query: 40  ITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEF 99
           + ELE+VF+++D NGDGKIS+ EL S++R+LG      E+ +M+DE D+D DGF++L EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 100 VELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVLQSLGDDC 159
              +               D E   E  LREAF +YD D NG ISA ELH+VL+ LGD C
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 160 SVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           SVADC +MI  VD++GDG ++F+EFK MM  G
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]
BLAST of CSPI05G27880 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 3.9e-95
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGS 186
           SVAGS
Sbjct: 181 SVAGS 185

[Close]
BLAST of CSPI05G27880 vs. TrEMBL
Match: A0A059D691_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02672 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.0e-60
Identity = 125/180 (69.44%), Postives = 148/180 (82.22%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSST-LGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60
           MG RSLFR+KKP S    P  S ++T LGSRSS     SQI ELE+VF+KFD NGDGKIS
Sbjct: 1   MGLRSLFRRKKPTSPSPPPGASGAATPLGSRSSSMGRRSQIEELEQVFRKFDANGDGKIS 60

Query: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           ++ELGSIM SLGH  TEEEL KM+ E D+DGDGFI+  EFVELNTK +DP E +ENLR+A
Sbjct: 61  ASELGSIMSSLGHPETEEELAKMVREVDSDGDGFIDFDEFVELNTKGVDPAEAMENLRDA 120

Query: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           FSVYDIDG+GSI+AEELHKV++SLG++ S+A+CR+MI GVDS+GDGMISFEEFKVMM  G
Sbjct: 121 FSVYDIDGDGSITAEELHKVMRSLGEENSLAECRKMIGGVDSDGDGMISFEEFKVMMMVG 180

[Close]
BLAST of CSPI05G27880 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.0e-60
Identity = 128/181 (70.72%), Postives = 151/181 (83.43%), Query Frame = 1 

Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSS-STLGSRSSLDDSESQITELEEVFKKFDVNGDGKI 60
           MGF+SLF RKKK  SS +SPL S++   LGSR+       QI ELE+VF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  SSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLRE 120
            S+ELGSIM SLG  ATEEEL  MI E DADGDG+I+L EF+ELNTK +D +E+LENL++
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMST 180
           AFSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+GVD NGDGMISF+EFKVMM +
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 174

[Close]
BLAST of CSPI05G27880 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 4.9e-58
Identity = 125/191 (65.45%), Postives = 151/191 (79.06%), Query Frame = 1 

Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSL-----------DDSESQITELEEVFK 60
           MGF S+F RKKK  S  N+  ++ S  +G+  S            + +++Q+ ELEEVFK
Sbjct: 1   MGFGSIFNRKKKRHSLSNASNLTGSPAVGANGSSFLQSPMRTPTRNLTQTQLQELEEVFK 60

Query: 61  KFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEID 120
           KFDVNGDGKISS+ELGSIM SLGH  +E+EL KMI EFDADGDG IN  EFVELNTK +D
Sbjct: 61  KFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFKEFVELNTKGVD 120

Query: 121 PEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMIS 180
            +E+LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MISGVDS+GDGMI 
Sbjct: 121 SDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMISGVDSDGDGMID 180

[Close]
BLAST of CSPI05G27880 vs. TrEMBL
Match: B9HW20_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 8.4e-58
Identity = 121/183 (66.12%), Postives = 147/183 (80.33%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPF----SSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDG 60
           MGF++LF +KK      SSV+SP    +S  GS+S       Q+ ELE+VFKKFDVNGDG
Sbjct: 1   MGFKALFNRKKKSKKSDSSVDSPSAIVASISGSKSPSIRLRPQVEELEQVFKKFDVNGDG 60

Query: 61  KISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENL 120
           KISSAELGSIM +LGH ATE+EL  MI EFDADGDGFI+L EFV LNT+ +D  E++ENL
Sbjct: 61  KISSAELGSIMANLGHEATEDELQTMITEFDADGDGFIDLQEFVALNTQGVDTNEVMENL 120

Query: 121 REAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMM 180
           ++AFSVYD+DGNGSISAEELHKV+ SLG+ CS+++CR++ISGVDS+GDGMI FEEFKVMM
Sbjct: 121 KDAFSVYDVDGNGSISAEELHKVMASLGEPCSMSECRKIISGVDSDGDGMIDFEEFKVMM 180

[Close]
BLAST of CSPI05G27880 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 207.2 bits (526), Expect = 8.6e-54
Identity = 101/143 (70.63%), Postives = 120/143 (83.92%), Query Frame = 1 

Query: 37  ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IM SLGH   EEEL K I E D  GDG+IN 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of CSPI05G27880 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 141.4 bits (355), Expect = 5.8e-34
Identity = 72/142 (50.70%), Postives = 103/142 (72.54%), Query Frame = 1 

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 101
           ++++VF++FD NGDGKIS  EL  ++R+L   A+ EE   M+ +FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 102 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 161
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH V+++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 162 GVDSNGDGMISFEEFKVMMSTG 180
            VD +GDG ++F+EFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of CSPI05G27880 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 141.4 bits (355), Expect = 5.8e-34
Identity = 74/144 (51.39%), Postives = 99/144 (68.75%), Query Frame = 1 

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 101
           EL++VF KFD NGDGKIS +ELG++ +S+G + TEEEL +++DE D D DGFIN  EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 161
           +        E+    REAF +YD + NG IS+ E+HKVL  LG  CSV DC +MI  VD+
Sbjct: 80  ICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 162 NGDGMISFEEFKVMMSTGLSVAGS 186
           +GDG ++FEEF+ MMS+   V G+
Sbjct: 140 DGDGNVNFEEFQKMMSSPELVKGT 159

[Close]
BLAST of CSPI05G27880 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 136.7 bits (343), Expect = 1.4e-32
Identity = 72/150 (48.00%), Postives = 104/150 (69.33%), Query Frame = 1 

Query: 29  SRSSLDDSESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDA 88
           SR+ L   E    ++++VF++FD N DGKIS  EL  ++ +L  NA++EE   M+ EFD 
Sbjct: 6   SRNCLGSME----DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 65

Query: 89  DGDGFINLHEFVELN--TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDD 148
           DG+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH V+++LG+ 
Sbjct: 66  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 125

Query: 149 CSVADCRQMISGVDSNGDGMISFEEFKVMM 177
           CS+ DC++MI+ VDS+GDG + FEEFK MM
Sbjct: 126 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]
BLAST of CSPI05G27880 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 134.4 bits (337), Expect = 7.1e-32
Identity = 69/137 (50.36%), Postives = 94/137 (68.61%), Query Frame = 1 

Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 101
           EL++VF +FD NGDGKIS  ELG + +++G + TE EL ++++E D D DG+INL EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 161
           L        E+    R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 162 NGDGMISFEEFKVMMST 179
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

[Close]
BLAST of CSPI05G27880 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 355.5 bits (911), Expect = 5.5e-95
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGS 186
           SVAGS
Sbjct: 181 SVAGS 185

[Close]
BLAST of CSPI05G27880 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 344.0 bits (881), Expect = 1.7e-91
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLD SESQI ELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEELGKMI+EFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

[Close]
BLAST of CSPI05G27880 vs. NCBI nr
Match: gi|702273734|ref|XP_010043960.1| (PREDICTED: probable calcium-binding protein CML25 [Eucalyptus grandis])

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-59
Identity = 125/180 (69.44%), Postives = 148/180 (82.22%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSST-LGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60
           MG RSLFR+KKP S    P  S ++T LGSRSS     SQI ELE+VF+KFD NGDGKIS
Sbjct: 1   MGLRSLFRRKKPTSPSPPPGASGAATPLGSRSSSMGRRSQIEELEQVFRKFDANGDGKIS 60

Query: 61  SAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           ++ELGSIM SLGH  TEEEL KM+ E D+DGDGFI+  EFVELNTK +DP E +ENLR+A
Sbjct: 61  ASELGSIMSSLGHPETEEELAKMVREVDSDGDGFIDFDEFVELNTKGVDPAEAMENLRDA 120

Query: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           FSVYDIDG+GSI+AEELHKV++SLG++ S+A+CR+MI GVDS+GDGMISFEEFKVMM  G
Sbjct: 121 FSVYDIDGDGSITAEELHKVMRSLGEENSLAECRKMIGGVDSDGDGMISFEEFKVMMMVG 180

[Close]
BLAST of CSPI05G27880 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-59
Identity = 128/181 (70.72%), Postives = 151/181 (83.43%), Query Frame = 1 

Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSS-STLGSRSSLDDSESQITELEEVFKKFDVNGDGKI 60
           MGF+SLF RKKK  SS +SPL S++   LGSR+       QI ELE+VF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT-------QIAELEQVFNKFDVNGDGKI 60

Query: 61  SSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLRE 120
            S+ELGSIM SLG  ATEEEL  MI E DADGDG+I+L EF+ELNTK +D +E+LENL++
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMST 180
           AFSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+GVD NGDGMISF+EFKVMM +
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 174

[Close]
BLAST of CSPI05G27880 vs. NCBI nr
Match: gi|743822788|ref|XP_011021780.1| (PREDICTED: probable calcium-binding protein CML25 [Populus euphratica])

HSP 1 Score: 235.7 bits (600), Expect = 6.4e-59
Identity = 124/183 (67.76%), Postives = 148/183 (80.87%), Query Frame = 1 

Query: 1   MGFRSLFRKKKPF----SSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDG 60
           MGF++LF +KK      SSV+SP    +ST GS+S       Q+ ELE+VFKKFDVNGDG
Sbjct: 1   MGFKALFNRKKKSKKSDSSVDSPSAIVTSTNGSKSPSIRLRPQVQELEQVFKKFDVNGDG 60

Query: 61  KISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENL 120
           KISSAELGSIM SLGH ATE+EL  MI EFDADGDGFI+L EFV LNT+ +D  E++ENL
Sbjct: 61  KISSAELGSIMASLGHEATEDELKTMITEFDADGDGFIDLQEFVALNTQGVDTNEVMENL 120

Query: 121 REAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMM 180
           ++AFSVYD+DGNGSISAEELHKV+ SLG+ CS+A+CR++ISGVDS+GDGMI FEEFKVMM
Sbjct: 121 KDAFSVYDVDGNGSISAEELHKVMASLGEPCSMAECRKIISGVDSDGDGMIDFEEFKVMM 180

The following BLAST results are available for this feature:
BLAST of CSPI05G27880 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML25_ARATH1.5e-5270.63Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ3.0e-4052.78Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
CML15_ORYSJ3.8e-3552.52Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ... [more]
POLC2_JUNOX3.2e-3448.75Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2[more]
CML18_ORYSJ4.6e-3349.34Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of CSPI05G27880 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KX11_CUCSA3.9e-95100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1[more]
A0A059D691_EUCGR9.0e-6069.44Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B02672 PE=4 SV=1[more]
A0A0A0KST2_CUCSA9.0e-6070.72Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1[more]
A0A061E9N9_THECC4.9e-5865.45Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1[more]
B9HW20_POPTR8.4e-5866.12Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2[more]
BLAST of CSPI05G27880 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.18.6e-5470.63 EF hand calcium-binding protein family[more]
AT5G37770.15.8e-3450.70 EF hand calcium-binding protein family[more]
AT1G73630.15.8e-3451.39 EF hand calcium-binding protein family[more]
AT1G66400.11.4e-3248.00 calmodulin like 23[more]
AT1G18210.17.1e-3250.36 Calcium-binding EF-hand family protein[more]
BLAST of CSPI05G27880 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449242|ref|XP_004142374.1|5.5e-95100.00PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|659129528|ref|XP_008464718.1|1.7e-9197.28PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|702273734|ref|XP_010043960.1|1.3e-5969.44PREDICTED: probable calcium-binding protein CML25 [Eucalyptus grandis][more]
gi|778703916|ref|XP_011655449.1|1.3e-5970.72PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|743822788|ref|XP_011021780.1|6.4e-5967.76PREDICTED: probable calcium-binding protein CML25 [Populus euphratica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048767 root hair elongation
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008150 biological_process
biological_process GO:0009409 response to cold
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0044424 intracellular part
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G27880.1CSPI05G27880.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 114..176
score: 1.9E-12coord: 42..102
score: 1.3
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..143
score: 7.2E-6coord: 78..106
score: 2.7E-4coord: 42..70
score: 1.6E-7coord: 151..179
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 74..109
score: 13.035coord: 111..146
score: 15.378coord: 147..182
score: 12.087coord: 38..73
score: 16
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 36..177
score: 4.2
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 35..177
score: 3.39
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
score: -coord: 87..99
score: -coord: 160..172
score: -coord: 51..63
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 29..179
score: 4.3
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 29..179
score: 4.3