CsaV3_5G037010 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_5G037010
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Calcium-binding protein
Location	chr5 : 29197644 .. 29198992 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGGGTGAGTGTAGGGTAAGAGTTTTTCCTCTCAAGCTTTCTATATATATTCATAATAGTCTACTCCAAAAACAAGGAAATGATATAGATATATATAGAAAGCTAGAGTTTTGTGTTGCCAACTCATCCTCTTCTACGATAATTTTTTATTCTCAAGTGAAAGATGCATCTTATCATATGATGATACATTCTCCCACAATATATAGAAAAAAATAAAATAAAATCTCAACCTCAGTTAGTGCCATTCCACCATTTGTTAATCTCTCTCTCTCGTCCATTTCTCTATAATTGGATTGGTATTCAACAAACTGTAGCCAATCTCAAATCCCCATTTTCCTTCCCCGCTTCAATTCTCCCCTAAATCGATAATGCTCAATTTCTCCTTCCTGTTCATCCTCAATCCTCCGATTTGCATTTCTTTCTGATATCGGTTTTGGTGGTAATTAATTCATCGGCTGTTCTTCGCAGCAGAGAGAAATAATTCGACCATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGAGTTTGAACATCCGCTGGTAGATTTCAAGAGGAATTTGCTAGAATTCGGATTTTTTTTTCTTCTTCCGTTCTGTCTCCGACGAAAGTACTGGAAGTTCTTCACCGTTATATATATATATATTATTTGTAGAATTGTAAAAAACATAAAAAAATAATAATAAATATTTATTCTATAAATAATAGACACTTACATGACTAATTGATAGTCAATAAATAAAATTCTATCCATTTTTATTCTATTTATACATTCCAAAATTTTAAATCCATAAATATTTTAATTTATTTTGTTCTATTTAAAAATAT

mRNA sequence

ATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

Coding sequence (CDS)

ATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCACTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGATTCAGAATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

Protein sequence

MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVAGS

Homology

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BLAST of CsaV3_5G037010 vs. NCBI nr
Match: XP_004142374.1 (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus] >KGN52351.1 hypothetical protein Csa_5G627080 [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 2.4e-78
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGS 186
           SVAGS
Sbjct: 181 SVAGS 185

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BLAST of CsaV3_5G037010 vs. NCBI nr
Match: XP_022157783.1 (probable calcium-binding protein CML25, partial [Momordica charantia])

HSP 1 Score: 230.3 bits (586), Expect = 5.2e-57
Identity = 132/191 (69.11%), Postives = 144/191 (75.39%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLV--SSSSTLGSR-----SSLDDSESQITELEEVFKKFDV 60
           MGFRSLF RKKK ++S+ SP+V  ++S+ LGSR              I ELEEVFKKFDV
Sbjct: 1   MGFRSLFYRKKKSYASIKSPIVVSTASARLGSRXXXXXXXXXXXXXXIAELEEVFKKFDV 60

Query: 61  NGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEEL 120
           NGDGKISSAELGSIM SLGH                       L+EFVELNTKEIDPEEL
Sbjct: 61  NGDGKISSAELGSIMSSLGHRATEEDLAKMIEEFDADGDGFIDLNEFVELNTKEIDPEEL 120

Query: 121 LENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEF 180
           LENLREAFSVYDIDGNGSISAEELH+VL+SLGDDC+V DCRQMIS VDSNGDGMISF+EF
Sbjct: 121 LENLREAFSVYDIDGNGSISAEELHEVLRSLGDDCTVDDCRQMISAVDSNGDGMISFDEF 180

Query: 181 KVMMSTGLSVA 184
           KVMMSTGLSVA
Sbjct: 181 KVMMSTGLSVA 191

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BLAST of CsaV3_5G037010 vs. NCBI nr
Match: XP_022990173.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 227.3 bits (578), Expect = 4.4e-56
Identity = 152/185 (82.16%), Postives = 163/185 (88.11%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLGS SSL+DS +QI ELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREA 120
           S            XXXXXXXXXXXXXXXXXXXXXXX   FVELNTKEIDPEELLE+LREA
Sbjct: 61  SXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFVELNTKEIDPEELLESLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMI+GVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMINGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVA 184
           GLSVA
Sbjct: 181 GLSVA 185

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BLAST of CsaV3_5G037010 vs. NCBI nr
Match: XP_023549905.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210.7 bits (535), Expect = 4.3e-51
Identity = 140/185 (75.68%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60
           M F+ LF  KKKPF+SV S +VSS ST G RSS  DS S+  ELEEVFKKFDVNGDGKIS
Sbjct: 1   MRFKCLFCWKKKPFASVTSSIVSSPSTNGPRSSSRDSHSRNAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREA 120
           SAELGSIM SLGH    XXXXXXXXXXXXXXXXXXXL EFVELNTKEIDPEELL+NLREA
Sbjct: 61  SAELGSIMGSLGHKATEXXXXXXXXXXXXXXXXXXXLQEFVELNTKEIDPEELLDNLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           F              ELHKV++SLGDDCSVADCRQMISGVD NGDG+ISF+EFKVMMS G
Sbjct: 121 FXXXXXXXXXXXXXXELHKVMRSLGDDCSVADCRQMISGVDRNGDGLISFDEFKVMMSAG 180

Query: 181 LSVAG 185
           LSVAG
Sbjct: 181 LSVAG 185

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BLAST of CsaV3_5G037010 vs. NCBI nr
Match: XP_008464718.1 (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 205.3 bits (521), Expect = 1.8e-49
Identity = 141/184 (76.63%), Postives = 142/184 (77.17%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSP                    I ELEEVFKKFDVNGD     
Sbjct: 1   MGFRSLFRKKKPFSSVNSPXXXXXXXXXXXXXXXXXXXXIAELEEVFKKFDVNGDXXXXX 60

Query: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120
                       XXXXXXXXXXXXXXXXXXXXXXX    VELNTKEIDPEELLENLREAF
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

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BLAST of CsaV3_5G037010 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 175.3 bits (443), Expect = 3.6e-44
Identity = 88/143 (61.54%), Postives = 106/143 (74.13%), Query Frame = 0


Query: 37  ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IM SLGH                        
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CsaV3_5G037010 vs. TAIR10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 102.4 bits (254), Expect = 3.0e-22
Identity = 58/137 (42.34%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVE 101
           EL++VF +FD NGDGKIS  ELG + +++G                        L EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 161
           L        E    +R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 162 NGDGMISFEEFKVMMST 179
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

[Close]

BLAST of CsaV3_5G037010 vs. TAIR10
Match: AT1G73630.1 (EF hand calcium-binding protein family)

HSP 1 Score: 98.6 bits (244), Expect = 4.3e-21
Identity = 81/144 (56.25%), Postives = 101/144 (70.14%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVE 101
           EL++VF KFD NGDGKIS +ELG++ +S+G XXXXXXXXXXXXXXXXXXXXXXX      
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 79

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 161
                    E    +REAF +YD + NG IS+ E+HKVL  LG  CSV DC +MI  VD+
Sbjct: 80  XXXXXXXXVE----IREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 162 NGDGMISFEEFKVMMSTGLSVAGS 186
           +GDG ++FEEF+ MMS+   V G+
Sbjct: 140 DGDGNVNFEEFQKMMSSPELVKGT 159

[Close]

BLAST of CsaV3_5G037010 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 94.0 bits (232), Expect = 1.1e-19
Identity = 55/140 (39.29%), Postives = 79/140 (56.43%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVE 101
           EL  VF+ FD NGDGKI+  EL    +S+G                        + EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLG--DDCSVADCRQMISGV 161
           L  + ++ +E  E++REAF V+D +G+G I+ EEL  VL S+G     ++ DC++MIS V
Sbjct: 65  LYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKV 124

Query: 162 DSNGDGMISFEEFKVMMSTG 180
           D +GDGM++F+EFK MM  G
Sbjct: 125 DVDGDGMVNFKEFKQMMRGG 144

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BLAST of CsaV3_5G037010 vs. TAIR10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.4e-18
Identity = 68/155 (43.87%), Postives = 98/155 (63.23%), Query Frame = 0


Query: 24  SSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXX 83
           SS L S      S+  + E+  VF +FD++ DGKIS  E   ++R+LG       XXXXX
Sbjct: 32  SSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQERAIEDXXXXX 91

Query: 84  XXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSL 143
           XXXXXXXXXXXX  EF++   +         ++R +F  +D++G+G ISAEE+  VL  L
Sbjct: 92  XXXXXXXXXXXXFREFIDAYKRSGGIRS--SDIRNSFWTFDLNGDGKISAEEVMSVLWKL 151

Query: 144 GDDCSVADCRQMISGVDSNGDGMISFEEFKVMMST 179
           G+ CS+ DC +M+  VD++GDG+++ EEF  MMS+
Sbjct: 152 GERCSLEDCNRMVRAVDADGDGLVNMEEFIKMMSS 184

[Close]

BLAST of CsaV3_5G037010 vs. Swiss-Prot
Match: sp|Q9FYK2|CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.5e-43
Identity = 88/143 (61.54%), Postives = 106/143 (74.13%), Query Frame = 0


Query: 37  ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IM SLGH                        
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of CsaV3_5G037010 vs. Swiss-Prot
Match: sp|Q0DJV6|CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.6e-25
Identity = 66/152 (43.42%), Postives = 85/152 (55.92%), Query Frame = 0


Query: 40  ITELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEF 99
           + ELE+VF+++D NGDGKIS+ EL S++R+LG                        L EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 100 VELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVLQSLGDDC 159
              +               D E   E  LREAF +YD D NG ISA ELH+VL+ LGD C
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 160 SVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           SVADC +MI  VD++GDG ++F+EFK MM  G
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]

BLAST of CsaV3_5G037010 vs. Swiss-Prot
Match: sp|Q6L4D4|CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.4e-23
Identity = 82/139 (58.99%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVE 101
           E E VF+KFD NGDG+IS AEL ++ RS+GH XXXXXXXXXXXXXXXXXXXXXX      
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAXXXXXXXXXXXXXXXXXXXXXXXXXXXX 114

Query: 102 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVD 161
            +     D     E+LR AF V+D DGNG I+  EL +VL+ +G+  +VA CR+MI GVD
Sbjct: 115 XSAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 162 SNGDGMISFEEFKVMMSTG 180
            NGDG+I+FEEFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]

BLAST of CsaV3_5G037010 vs. Swiss-Prot
Match: sp|O64943|POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 2.4e-21
Identity = 80/160 (50.00%), Postives = 105/160 (65.62%), Query Frame = 0


Query: 25  STLGSRSSLDDS---ESQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXX 84
           S+ GS+S+       E  + ELEEVFKKFD NGDGKIS +EL  I+RSLG       XXX
Sbjct: 6   SSDGSKSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEXXX 65

Query: 85  XXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQ 144
           XXXXXXXXXXXXXX       N K       +++L+ AF V+D D NGSISA EL   L+
Sbjct: 66  XXXXXXXXXXXXXXXXXXXXXNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLE 125

Query: 145 SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLS 182
           S+G+ C++ + + +I  VD NGDG+IS EEF+ MM++ ++
Sbjct: 126 SVGEPCTIEESKNIIHNVDKNGDGLISVEEFQTMMTSEMT 161

[Close]

BLAST of CsaV3_5G037010 vs. Swiss-Prot
Match: sp|Q9LE22|CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.4e-21
Identity = 58/137 (42.34%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVE 101
           EL++VF +FD NGDGKIS  ELG + +++G                        L EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 161
           L        E    +R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 162 NGDGMISFEEFKVMMST 179
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

[Close]

BLAST of CsaV3_5G037010 vs. TrEMBL
Match: tr|A0A0A0KX11|A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.6e-78
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGS 186
           SVAGS
Sbjct: 181 SVAGS 185

[Close]

BLAST of CsaV3_5G037010 vs. TrEMBL
Match: tr|A0A1S3CNQ0|A0A1S3CNQ0_CUCME (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.2e-49
Identity = 141/184 (76.63%), Postives = 142/184 (77.17%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSP                    I ELEEVFKKFDVNGD     
Sbjct: 1   MGFRSLFRKKKPFSSVNSPXXXXXXXXXXXXXXXXXXXXIAELEEVFKKFDVNGDXXXXX 60

Query: 61  AELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREAF 120
                       XXXXXXXXXXXXXXXXXXXXXXX    VELNTKEIDPEELLENLREAF
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

[Close]

BLAST of CsaV3_5G037010 vs. TrEMBL
Match: tr|A0A0A0KST2|A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.0e-48
Identity = 112/181 (61.88%), Postives = 133/181 (73.48%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSS-STLGSRSSLDDSESQITELEEVFKKFDVNGDGKI 60
           MGF+SLF RKKK  SS +SPL S++   LGSR       +QI ELE+VF KFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSR-------TQIAELEQVFNKFDVNGDGKI 60

Query: 61  SSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLRE 120
            S+ELGSIM SLG                        L EF+ELNTK +D +E+LENL++
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMST 180
           AFSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+GVD NGDGMISF+EFKVMM +
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMS 174

[Close]

BLAST of CsaV3_5G037010 vs. TrEMBL
Match: tr|A0A1S3CSR2|A0A1S3CSR2_CUCME (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-46
Identity = 110/180 (61.11%), Postives = 130/180 (72.22%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKK   S +SPL   S+TL     +  S +QI ELE+VF KFDVNGDGKI 
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPL--GSTTL----PIFGSRTQIAELEQVFNKFDVNGDGKIC 60

Query: 61  SAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLREA 120
           S+ELGSIM SLG                        L EF+ELNTK +D  E+LENL++A
Sbjct: 61  SSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDA 120

Query: 121 FSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+GVD NGDGMISF+EFKVMM +G
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSG 174

[Close]

BLAST of CsaV3_5G037010 vs. TrEMBL
Match: tr|W9SF34|W9SF34_9ROSA (Putative calcium-binding protein CML25 OS=Morus notabilis OX=981085 GN=L484_000710 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 8.0e-46
Identity = 127/181 (70.17%), Postives = 150/181 (82.87%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSST-LGSRSSLDDSESQITELEEVFKKFDVNGDGKI 60
           MGF+SLF R+KKP SS  +P  S +     SRS   +  +QI ELE+VFKKFDVNGDGKI
Sbjct: 1   MGFKSLFGRRKKPDSSTANPNPSPAEVPFASRSPSVNGRAQIEELEQVFKKFDVNGDGKI 60

Query: 61  SSAELGSIMRSLGHXXXXXXXXXXXXXXXXXXXXXXXLHEFVELNTKEIDPEELLENLRE 120
           SS+ELG+IM SLG     XXXXXXXXXXXXXXXXXXXL EF+ELNTK +D +E+LENL++
Sbjct: 61  SSSELGAIMGSLGQPSGEXXXXXXXXXXXXXXXXXXXLKEFIELNTKGVDSDEVLENLKD 120

Query: 121 AFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMST 180
           AFSVYDID NGSISAEEL++VL+SLGD+CS+A+CR+MISGVD +GDG ISFEEFKVMM T
Sbjct: 121 AFSVYDIDKNGSISAEELYEVLRSLGDECSIAECRKMISGVDCDGDGTISFEEFKVMMMT 180

The following BLAST results are available for this feature:

BLAST of CsaV3_5G037010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142374.1	2.4e-78	100.00	PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus] >KGN52351.1 ...	[more]
XP_022157783.1	5.2e-57	69.11	probable calcium-binding protein CML25, partial [Momordica charantia]	[more]
XP_022990173.1	4.4e-56	82.16	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]
XP_023549905.1	4.3e-51	75.68	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
XP_008464718.1	1.8e-49	76.63	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo]	[more]

BLAST of CsaV3_5G037010 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24620.1	3.6e-44	61.54	EF hand calcium-binding protein family	[more]
AT1G18210.1	3.0e-22	42.34	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	4.3e-21	56.25	EF hand calcium-binding protein family	[more]
AT4G12860.1	1.1e-19	39.29	EF hand calcium-binding protein family	[more]
AT2G15680.1	3.4e-18	43.87	Calcium-binding EF-hand family protein	[more]

BLAST of CsaV3_5G037010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FYK2\|CML25_ARATH	6.5e-43	61.54	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
sp\|Q0DJV6\|CML18_ORYSJ	3.6e-25	43.42	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q6L4D4\|CML15_ORYSJ	4.4e-23	58.99	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|O64943\|POLC2_JUNOX	2.4e-21	50.00	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
sp\|Q9LE22\|CML27_ARATH	5.4e-21	42.34	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]

BLAST of CsaV3_5G037010 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KX11\|A0A0A0KX11_CUCSA	1.6e-78	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1	[more]
tr\|A0A1S3CNQ0\|A0A1S3CNQ0_CUCME	1.2e-49	76.63	probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 P...	[more]
tr\|A0A0A0KST2\|A0A0A0KST2_CUCSA	5.0e-48	61.88	Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1	[more]
tr\|A0A1S3CSR2\|A0A1S3CSR2_CUCME	1.2e-46	61.11	probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 P...	[more]
tr\|W9SF34\|W9SF34_9ROSA	8.0e-46	70.17	Putative calcium-binding protein CML25 OS=Morus notabilis OX=981085 GN=L484_0007...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0048767	root hair elongation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009409	response to cold
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G037010.1	CsaV3_5G037010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 151..179 e-value: 4.0E-5 score: 33.0 coord: 115..143 e-value: 7.2E-6 score: 35.5 coord: 78..106 e-value: 2.7E-4 score: 30.3 coord: 42..70 e-value: 1.6E-7 score: 41.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 42..102 e-value: 1.6E-13 score: 50.8 coord: 114..176 e-value: 2.6E-12 score: 46.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 16.578
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 147..182 score: 12.087
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 13.035
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 111..146 score: 15.378
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 116..177 e-value: 1.00596E-18 score: 75.6621
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 42..102 e-value: 2.79273E-18 score: 74.5065
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 69..103 e-value: 1.3E-13 score: 52.6
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 104..181 e-value: 1.9E-25 score: 90.9
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 3..68 e-value: 4.0E-9 score: 38.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..36
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 33..178
None	No IPR available	PANTHER	PTHR10891:SF738	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 33..178
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 160..172
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 124..136
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 35..177

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_5G037010	CSPI05G27880	Wild cucumber (PI 183967)	cpicucB284
CsaV3_5G037010	Cucsa.029820	Cucumber (Gy14) v1	cgycucB005
CsaV3_5G037010	CmaCh04G000750	Cucurbita maxima (Rimu)	cmacucB0894
CsaV3_5G037010	CmaCh04G010210	Cucurbita maxima (Rimu)	cmacucB0896
CsaV3_5G037010	CmoCh04G000710	Cucurbita moschata (Rifu)	cmocucB0883
CsaV3_5G037010	Cp4.1LG01g07490	Cucurbita pepo (Zucchini)	cpecucB0558
CsaV3_5G037010	CsGy5G027280	Cucumber (Gy14) v2	cgybcucB232
CsaV3_5G037010	Carg22019	Silver-seed gourd	carcucB0206
CsaV3_5G037010	Cla003404	Watermelon (97103) v1	cucwmB417
CsaV3_5G037010	MELO3C026437	Melon (DHL92) v3.5.1	cucmeB386
CsaV3_5G037010	ClCG07G005670	Watermelon (Charleston Gray)	cucwcgB424
CsaV3_5G037010	Lsi04G011060	Bottle gourd (USVL1VR-Ls)	cuclsiB389
CsaV3_5G037010	MELO3C026437.2	Melon (DHL92) v3.6.1	cucmedB382
CsaV3_5G037010	Cla97C07G133920	Watermelon (97103) v2	cucwmbB409
CsaV3_5G037010	Bhi07G000337	Wax gourd	cucwgoB532

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_5G037010	Silver-seed gourd	carcucB0710
CsaV3_5G037010	Cucurbita moschata (Rifu)	cmocucB0891
CsaV3_5G037010	Cucurbita pepo (Zucchini)	cpecucB0552