Cucsa.029820 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.029820
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionEF hand calcium-binding protein family
Locationscaffold00399 : 60136 .. 60584 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

mRNA sequence

AATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

Coding sequence (CDS)

AATCTCAGATCACAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCCTCTGCTGAATTAGGCTCCATTATGAGAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGACGAATTCGATGCCGATGGCGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTGTTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCCATCTCTGCTGAGGAATTGCATAAGGTTCTGCAAAGTTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTGTCCGTCGCTGGCAGCTGA

Protein sequence

SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVAGS*
Homology
[Close]
BLAST of Cucsa.029820 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-53
Identity = 101/142 (71.13%), Postives = 119/142 (83.80%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           ++I ELE VFKKFDVNGDGKISS ELG+IM SLGH   EEEL K I E D  GDG+IN  
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD +GDG I FEEFK+MM+ G
Sbjct: 153 GVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of Cucsa.029820 vs. Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 2.8e-41
Identity = 86/143 (60.14%), Postives = 107/143 (74.83%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           E+E VF+KFD NGDG+IS +ELG++  SLGH AT++EL +M+ E DADGDGFI+L EF  
Sbjct: 40  EMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAA 99

Query: 65  LN-TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVD 124
           LN T   D   + E+LR AF V+D DGNG+ISA EL +VL  LG+  +V  CR+MI GVD
Sbjct: 100 LNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVD 159

Query: 125 SNGDGMISFEEFKVMMSTGLSVA 147
            NGDG+ISFEEFKVMM+ G S A
Sbjct: 160 QNGDGLISFEEFKVMMAGGGSFA 182

[Close]
BLAST of Cucsa.029820 vs. Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 4.7e-36
Identity = 73/139 (52.52%), Postives = 101/139 (72.66%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           E E VF+KFD NGDG+IS AEL ++ RS+GH  T++E+ +M+ E D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 65  LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVD 124
           ++     D     E+LR AF V+D DGNG I+  EL +VL+ +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 125 SNGDGMISFEEFKVMMSTG 143
            NGDG+I+FEEFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]
BLAST of Cucsa.029820 vs. Swiss-Prot
Match: POLC2_JUNOX (Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 6.7e-35
Identity = 73/142 (51.41%), Postives = 103/142 (72.54%), Query Frame = 1 

Query: 3   ITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEF 62
           + ELEEVFKKFD NGDGKIS +EL  I+RSLG +  E E+  M++E DADGDG+++L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 63  VELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGV 122
           V+LN K       +++L+ AF V+D D NGSISA EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 123 DSNGDGMISFEEFKVMMSTGLS 145
           D NGDG+IS EEF+ MM++ ++
Sbjct: 144 DKNGDGLISVEEFQTMMTSEMT 161

[Close]
BLAST of Cucsa.029820 vs. Swiss-Prot
Match: CML18_ORYSJ (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.7e-34
Identity = 75/152 (49.34%), Postives = 100/152 (65.79%), Query Frame = 1 

Query: 3   ITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEF 62
           + ELE+VF+++D NGDGKIS+ EL S++R+LG      E+ +M+DE D+D DGF++L EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 63  VELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVLQSLGDDC 122
              +               D E   E  LREAF +YD D NG ISA ELH+VL+ LGD C
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 123 SVADCRQMISGVDSNGDGMISFEEFKVMMSTG 143
           SVADC +MI  VD++GDG ++F+EFK MM  G
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]
BLAST of Cucsa.029820 vs. TrEMBL
Match: A0A0A0KX11_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 1.4e-76
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH
Sbjct: 38  SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 97

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS
Sbjct: 98  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 157

Query: 121 GVDSNGDGMISFEEFKVMMSTGLSVAGS 149
           GVDSNGDGMISFEEFKVMMSTGLSVAGS
Sbjct: 158 GVDSNGDGMISFEEFKVMMSTGLSVAGS 185

[Close]
BLAST of Cucsa.029820 vs. TrEMBL
Match: A0A061E9N9_THECC (Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.7e-57
Identity = 111/142 (78.17%), Postives = 128/142 (90.14%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           +Q+ ELEEVFKKFDVNGDGKISS+ELGSIM SLGH  +E+EL KMI EFDADGDG IN  
Sbjct: 50  TQLQELEEVFKKFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFK 109

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTK +D +E+LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MIS
Sbjct: 110 EFVELNTKGVDSDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMIS 169

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVDS+GDGMI FEEFKVMM  G
Sbjct: 170 GVDSDGDGMIDFEEFKVMMMAG 191

[Close]
BLAST of Cucsa.029820 vs. TrEMBL
Match: A0A0A0KST2_CUCSA (Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 2.4e-55
Identity = 108/142 (76.06%), Postives = 127/142 (89.44%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           +QI ELE+VF KFDVNGDGKI S+ELGSIM SLG  ATEEEL  MI E DADGDG+I+L 
Sbjct: 33  TQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLD 92

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EF+ELNTK +D +E+LENL++AFSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+
Sbjct: 93  EFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 152

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD NGDGMISF+EFKVMM +G
Sbjct: 153 GVDKNGDGMISFDEFKVMMMSG 174

[Close]
BLAST of Cucsa.029820 vs. TrEMBL
Match: B9HW20_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 2.7e-54
Identity = 105/141 (74.47%), Postives = 125/141 (88.65%), Query Frame = 1 

Query: 2   QITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHE 61
           Q+ ELE+VFKKFDVNGDGKISSAELGSIM +LGH ATE+EL  MI EFDADGDGFI+L E
Sbjct: 43  QVEELEQVFKKFDVNGDGKISSAELGSIMANLGHEATEDELQTMITEFDADGDGFIDLQE 102

Query: 62  FVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISG 121
           FV LNT+ +D  E++ENL++AFSVYD+DGNGSISAEELHKV+ SLG+ CS+++CR++ISG
Sbjct: 103 FVALNTQGVDTNEVMENLKDAFSVYDVDGNGSISAEELHKVMASLGEPCSMSECRKIISG 162

Query: 122 VDSNGDGMISFEEFKVMMSTG 143
           VDS+GDGMI FEEFKVMM  G
Sbjct: 163 VDSDGDGMIDFEEFKVMMMMG 183

[Close]
BLAST of Cucsa.029820 vs. TrEMBL
Match: A0A0D2R7V3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G172400 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 4.5e-54
Identity = 103/142 (72.54%), Postives = 127/142 (89.44%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           +Q+ E+EEVFKKFD NGDGKIS++ELGSIM+SLG   ++EEL  MI EFDADGDGFIN +
Sbjct: 42  TQLQEIEEVFKKFDANGDGKISASELGSIMKSLGQQPSDEELHNMIKEFDADGDGFINFN 101

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EF+ELNTK +D EE+LENL++AFSVYDIDGNG ISAEELH+VL+SLG++CS+A+CR+MIS
Sbjct: 102 EFIELNTKGVDSEEVLENLKDAFSVYDIDGNGWISAEELHEVLKSLGEECSIAECRKMIS 161

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD++G+GMI FEEFKVMM  G
Sbjct: 162 GVDNDGNGMIDFEEFKVMMMAG 183

[Close]
BLAST of Cucsa.029820 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 209.9 bits (533), Expect = 1.1e-54
Identity = 101/142 (71.13%), Postives = 119/142 (83.80%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           ++I ELE VFKKFDVNGDGKISS ELG+IM SLGH   EEEL K I E D  GDG+IN  
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MI 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD +GDG I FEEFK+MM+ G
Sbjct: 153 GVDKDGDGTIDFEEFKIMMTMG 174

[Close]
BLAST of Cucsa.029820 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 144.1 bits (362), Expect = 7.2e-35
Identity = 72/142 (50.70%), Postives = 103/142 (72.54%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           ++++VF++FD NGDGKIS  EL  ++R+L   A+ EE   M+ +FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 65  LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 124
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH V+++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 125 GVDSNGDGMISFEEFKVMMSTG 143
            VD +GDG ++F+EFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]
BLAST of Cucsa.029820 vs. TAIR10
Match: AT1G73630.1 (AT1G73630.1 EF hand calcium-binding protein family)

HSP 1 Score: 143.7 bits (361), Expect = 9.4e-35
Identity = 74/144 (51.39%), Postives = 99/144 (68.75%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           EL++VF KFD NGDGKIS +ELG++ +S+G + TEEEL +++DE D D DGFIN  EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 65  LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 124
           +        E+    REAF +YD + NG IS+ E+HKVL  LG  CSV DC +MI  VD+
Sbjct: 80  ICRSSSSAVEI----REAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 125 NGDGMISFEEFKVMMSTGLSVAGS 149
           +GDG ++FEEF+ MMS+   V G+
Sbjct: 140 DGDGNVNFEEFQKMMSSPELVKGT 159


HSP 2 Score: 44.3 bits (103), Expect = 7.7e-05
Identity = 24/82 (29.27%), Postives = 43/82 (52.44%), Query Frame = 1 

Query: 63  VELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGV 122
           +E   K   P   +E L++ F  +D +G+G IS  EL  V +S+G   +  +  +++  +
Sbjct: 6   LESTNKSTTPSTDME-LKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEI 65

Query: 123 DSNGDGMISFEEFKVMMSTGLS 145
           D + DG I+ EEF  +  +  S
Sbjct: 66  DIDCDGFINQEEFATICRSSSS 86

[Close]
BLAST of Cucsa.029820 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 140.6 bits (353), Expect = 7.9e-34
Identity = 68/137 (49.64%), Postives = 99/137 (72.26%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           ++++VF++FD N DGKIS  EL  ++ +L  NA++EE   M+ EFD DG+GFI+L EFV 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 65  LN--TKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGV 124
           L   + +      + +L+EAF +YD+D NG ISA ELH V+++LG+ CS+ DC++MI+ V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 125 DSNGDGMISFEEFKVMM 140
           DS+GDG + FEEFK MM
Sbjct: 135 DSDGDGCVDFEEFKKMM 151

[Close]
BLAST of Cucsa.029820 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 136.7 bits (343), Expect = 1.1e-32
Identity = 69/137 (50.36%), Postives = 94/137 (68.61%), Query Frame = 1 

Query: 5   ELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVE 64
           EL++VF +FD NGDGKIS  ELG + +++G + TE EL ++++E D D DG+INL EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 65  LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDS 124
           L        E+    R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 125 NGDGMISFEEFKVMMST 142
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

[Close]
BLAST of Cucsa.029820 vs. NCBI nr
Match: gi|449449242|ref|XP_004142374.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 2.1e-76
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH
Sbjct: 38  SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 97

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS
Sbjct: 98  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 157

Query: 121 GVDSNGDGMISFEEFKVMMSTGLSVAGS 149
           GVDSNGDGMISFEEFKVMMSTGLSVAGS
Sbjct: 158 GVDSNGDGMISFEEFKVMMSTGLSVAGS 185

[Close]
BLAST of Cucsa.029820 vs. NCBI nr
Match: gi|659129528|ref|XP_008464718.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo])

HSP 1 Score: 284.6 bits (727), Expect = 9.6e-74
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           SQI ELEEVFKKFDVNGDGKISSAELGSIM SLGHNATEEELGKMI+EFDADGDGFINLH
Sbjct: 38  SQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLH 97

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMIS
Sbjct: 98  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMIS 157

Query: 121 GVDSNGDGMISFEEFKVMMSTGLSVAG 148
           GVDSNGDGMISFEEFKVMMSTGLSVAG
Sbjct: 158 GVDSNGDGMISFEEFKVMMSTGLSVAG 184

[Close]
BLAST of Cucsa.029820 vs. NCBI nr
Match: gi|590696790|ref|XP_007045260.1| (Polcalcin Jun o, putative [Theobroma cacao])

HSP 1 Score: 228.4 bits (581), Expect = 8.2e-57
Identity = 111/142 (78.17%), Postives = 128/142 (90.14%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           +Q+ ELEEVFKKFDVNGDGKISS+ELGSIM SLGH  +E+EL KMI EFDADGDG IN  
Sbjct: 50  TQLQELEEVFKKFDVNGDGKISSSELGSIMGSLGHKPSEDELQKMIKEFDADGDGSINFK 109

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EFVELNTK +D +E+LENL++AFSVYDIDGNGSISAEELH+VL+SLGD+CS+A+CR+MIS
Sbjct: 110 EFVELNTKGVDSDEVLENLKDAFSVYDIDGNGSISAEELHEVLKSLGDECSIAECRKMIS 169

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVDS+GDGMI FEEFKVMM  G
Sbjct: 170 GVDSDGDGMIDFEEFKVMMMAG 191

[Close]
BLAST of Cucsa.029820 vs. NCBI nr
Match: gi|1009154824|ref|XP_015895380.1| (PREDICTED: probable calcium-binding protein CML25 [Ziziphus jujuba])

HSP 1 Score: 226.5 bits (576), Expect = 3.1e-56
Identity = 110/142 (77.46%), Postives = 129/142 (90.85%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           SQI ELEEVFKKFDVNGDGKISS+ELGSIM SLG +AT++EL KMI E D+DGDGFI+L+
Sbjct: 47  SQIAELEEVFKKFDVNGDGKISSSELGSIMGSLGQHATDDELRKMIKEVDSDGDGFIDLN 106

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EF+ELNTK +D +E LENL++AFSVYDIDGNGSISAEELHKVL+SLGD+CS+A+CR+MIS
Sbjct: 107 EFIELNTKGVDSKEALENLKDAFSVYDIDGNGSISAEELHKVLRSLGDECSIAECRKMIS 166

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD +GDG ISFEEFK+MM  G
Sbjct: 167 GVDCDGDGTISFEEFKIMMMMG 188

[Close]
BLAST of Cucsa.029820 vs. NCBI nr
Match: gi|778703916|ref|XP_011655449.1| (PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus])

HSP 1 Score: 223.0 bits (567), Expect = 3.4e-55
Identity = 108/142 (76.06%), Postives = 127/142 (89.44%), Query Frame = 1 

Query: 1   SQITELEEVFKKFDVNGDGKISSAELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLH 60
           +QI ELE+VF KFDVNGDGKI S+ELGSIM SLG  ATEEEL  MI E DADGDG+I+L 
Sbjct: 33  TQIAELEQVFNKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLD 92

Query: 61  EFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMIS 120
           EF+ELNTK +D +E+LENL++AFSVYDIDGNGSI+AEELH+VL+SLGDDCS+ADCR+MI+
Sbjct: 93  EFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 152

Query: 121 GVDSNGDGMISFEEFKVMMSTG 143
           GVD NGDGMISF+EFKVMM +G
Sbjct: 153 GVDKNGDGMISFDEFKVMMMSG 174

The following BLAST results are available for this feature:
BLAST of Cucsa.029820 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML25_ARATH1.9e-5371.13Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
CML10_ORYSJ2.8e-4160.14Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 ... [more]
CML15_ORYSJ4.7e-3652.52Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 ... [more]
POLC2_JUNOX6.7e-3551.41Polcalcin Jun o 2 OS=Juniperus oxycedrus PE=1 SV=2[more]
CML18_ORYSJ5.7e-3449.34Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica GN=CML18 ... [more]
BLAST of Cucsa.029820 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KX11_CUCSA1.4e-76100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G627080 PE=4 SV=1[more]
A0A061E9N9_THECC5.7e-5778.17Polcalcin Jun o, putative OS=Theobroma cacao GN=TCM_011044 PE=4 SV=1[more]
A0A0A0KST2_CUCSA2.4e-5576.06Polcalcin Jun o OS=Cucumis sativus GN=Csa_5G561760 PE=4 SV=1[more]
B9HW20_POPTR2.7e-5474.47Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s11740g PE=4 SV=2[more]
A0A0D2R7V3_GOSRA4.5e-5472.54Uncharacterized protein OS=Gossypium raimondii GN=B456_002G172400 PE=4 SV=1[more]
BLAST of Cucsa.029820 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24620.11.1e-5471.13 EF hand calcium-binding protein family[more]
AT5G37770.17.2e-3550.70 EF hand calcium-binding protein family[more]
AT1G73630.19.4e-3551.39 EF hand calcium-binding protein family[more]
AT1G66400.17.9e-3449.64 calmodulin like 23[more]
AT1G18210.11.1e-3250.36 Calcium-binding EF-hand family protein[more]
BLAST of Cucsa.029820 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449242|ref|XP_004142374.1|2.1e-76100.00PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
gi|659129528|ref|XP_008464718.1|9.6e-7497.28PREDICTED: probable calcium-binding protein CML25 [Cucumis melo][more]
gi|590696790|ref|XP_007045260.1|8.2e-5778.17Polcalcin Jun o, putative [Theobroma cacao][more]
gi|1009154824|ref|XP_015895380.1|3.1e-5677.46PREDICTED: probable calcium-binding protein CML25 [Ziziphus jujuba][more]
gi|778703916|ref|XP_011655449.1|3.4e-5576.06PREDICTED: probable calcium-binding protein CML25 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0048767 root hair elongation
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005575 cellular_component
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.029820.1Cucsa.029820.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 77..139
score: 1.1E-12coord: 5..65
score: 7.5
IPR002048EF-hand domainSMARTSM00054efh_1coord: 5..33
score: 1.6E-7coord: 41..69
score: 2.7E-4coord: 114..142
score: 4.0E-5coord: 78..106
score: 7.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 37..72
score: 13.035coord: 110..145
score: 12.087coord: 74..109
score: 15.378coord: 1..36
score: 16
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 2..140
score: 1.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 2..140
score: 1.84
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 123..135
score: -coord: 87..99
score: -coord: 14..26
score: -coord: 50..62
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 2..142
score: 6.0
NoneNo IPR availablePANTHERPTHR10891:SF605CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 2..142
score: 6.0