CmaCh12G010860 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G010860
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Kunitz trypsin inhibitor
Location	Cma_Chr12 : 8584615 .. 8585619 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAATGGATATCTCTTCAAATTGGCTATAAATGTCACTACACATTTGCATCCCATAACCCATAAGTTTTCGAACAAATTAATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTATTATTACATCACTATCGAACTCAAAAACTCAATTTAATCAGTGATACCATTAGAAAACTCTTGATTATGTTATGAATTCTTAACCATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGACCATGCAATTTGTTGCAATTGCATTAAATTAAATAATACTCTTGTTAAGTATTTTAGTTTATTTGATTAAAAAAATAATTAATAAAGGAATTCCGACGTTTTAGATTTAGTTTCTATGTCATTTTTAGGGTAAGAGCTCGAGCCCACGGCCAGGAGATATTGTCCTATTTGGACTTTCGTTCAAGGTTTTAAAACGGGTCTCAAGATTATAAAAATGTTTCGTTCCCC

mRNA sequence

CAAAATGGATATCTCTTCAAATTGGCTATAAATGTCACTACACATTTGCATCCCATAACCCATAAGTTTTCGAACAAATTAATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGACCATGCAATTTGTTGCAATTGCATTAAATTAAATAATACTCTTGTTAAGTATTTTAGTTTATTTGATTAAAAAAATAATTAATAAAGGAATTCCGACGTTTTAGATTTAGTTTCTATGTCATTTTTAGGGTAAGAGCTCGAGCCCACGGCCAGGAGATATTGTCCTATTTGGACTTTCGTTCAAGGTTTTAAAACGGGTCTCAAGATTATAAAAATGTTTCGTTCCCC

Coding sequence (CDS)

ATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MMLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFVRA

Homology

[Close]

BLAST of CmaCh12G010860 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 92.4 bits (228), Expect = 5.8e-18
Identity = 71/203 (34.98%), Postives = 101/203 (49.75%), Query Frame = 1


Query: 6   SLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL--LTRKGD 65
           +L+A +   L+ AA S     PVLDI+G  L     Y+I P + D  G LT+   T  G 
Sbjct: 16  ALLAAAANPLLSAADSAPN--PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGT 75

Query: 66  -QCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSIC--ITSTAWRVG 125
             CP  V Q   +  +    + F P  P ED++R   DL + F AF  C   +ST WR+ 
Sbjct: 76  FVCPPRVVQTR-KEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLD 135

Query: 126 EADPTTGRRFVETG------DDDTATGYFRIDR-DNFGVYNIGWCPSDVPIRGRPRCGRA 185
           + D +TG+ FV  G        +T + +F+I+     G Y + +CP+ V    + +CG  
Sbjct: 136 KYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDV 195

Query: 186 GILI-EKGLRLLALDGSAFPFEF 196
           GI I +KG R LAL    F FEF
Sbjct: 196 GIYIDQKGRRRLALSDKPFAFEF 214

[Close]

BLAST of CmaCh12G010860 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.6e-13
Identity = 50/137 (36.50%), Postives = 69/137 (50.36%), Query Frame = 1


Query: 2   MLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 61
           ++  SL+A S+ C        A  PPV D EG  L  +  Y++ PA     G LT+  R 
Sbjct: 8   LILLSLLAISFSCS-------AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 67

Query: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPY----LPSEDMIREGRDLKVVFQAFSICITSTAW 121
              CP  V Q     ++ GF V FTP+     P +  IR   D+++ F A +IC+ ST W
Sbjct: 68  LP-CPLLVAQETDERRK-GFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 127

Query: 122 RVGEADPTTGRRFVETG 135
            VG+ +P TG R V TG
Sbjct: 128 HVGD-EPLTGARRVVTG 134

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BLAST of CmaCh12G010860 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 43/129 (33.33%), Postives = 68/129 (52.71%), Query Frame = 1


Query: 26  PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTF 85
           PPV D +G  L  +  Y++ PA     G LT+    G +CP  V Q     Q  G  V  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQ-EADGQRDGLPVRI 62

Query: 86  TPY--LPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGD--DDTATG 145
            P+   PS+ +IR   D+++ F+A++ C+ ST W + +++  +GRR V TG   D + +G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSPSG 122

Query: 146 ---YFRIDR 148
               FRI++
Sbjct: 123 RENAFRIEK 129

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BLAST of CmaCh12G010860 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.0e-09
Identity = 48/164 (29.27%), Postives = 76/164 (46.34%), Query Frame = 1


Query: 7   LVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGN-LTLLTRKGDQC 66
           L AF+        ++ A SP VLD +G  L+  V+Y++  +I+   G  L L    G  C
Sbjct: 11  LFAFTSKSYFFGVANAANSP-VLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 70

Query: 67  PFHVGQVPLRSQ-EIGFGVTFTPYLPSEDMIREGRDLKVVFQAFS--ICITSTAWRVGEA 126
           P  V  V  RS  + G  V F+     +D++R   D+ + F      +C TST WR+   
Sbjct: 71  PEIV--VQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNY 130

Query: 127 DPTTGRRFVETG------DDDTATGYFRIDRDNFGVYNIGWCPS 161
           D + G+ +V T         +T   +F+I++     Y   +CPS
Sbjct: 131 DNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]

BLAST of CmaCh12G010860 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.4e-08
Identity = 60/208 (28.85%), Postives = 88/208 (42.31%), Query Frame = 1


Query: 3   LCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKG 62
           L  S   F ++  +  A S      VLD  G P+   V+YFI PAI   AG    L R G
Sbjct: 7   LTLSFFLFVFITNLSLAFSNEDVEQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTG 66

Query: 63  D-QCPFHVGQVPLRSQEIGFGVTFTPYLP--SEDMIREGRDLKVVFQAFSICITSTAWRV 122
           D  CP  V Q     QE+  G+     +P  S  +I  G  +++ +     C  S+ W V
Sbjct: 67  DLTCPVTVLQ---DRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKWLV 126

Query: 123 GEADPTTGRRFVETGDDD------TATGYFRIDRDNFGV-YNIGWCPSDVPIRGRPRCGR 182
              D    +  V  G  +      T +G F+I++   G  Y +G+C     ++G P C  
Sbjct: 127 -FVDNVIQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFC-----VKGSPTCLD 186

Query: 183 AGIL--IEKGLRLLALDGSAFPFEFVRA 199
            G     E G RL   +  +F   F++A
Sbjct: 187 VGRFDNDEDGRRLNLTEHESFQVVFIQA 205

[Close]

BLAST of CmaCh12G010860 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 2.1e-83
Identity = 153/200 (76.50%), Postives = 169/200 (84.50%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           M   KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860 vs. TrEMBL
Match: D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860 vs. TrEMBL
Match: G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 118/195 (60.51%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITD+AG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860 vs. TrEMBL
Match: B9N8J2_POPTR (Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPTR_0004s00220g PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 9.3e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 86.3 bits (212), Expect = 2.4e-17
Identity = 61/195 (31.28%), Postives = 90/195 (46.15%), Query Frame = 1


Query: 15  LVMAASSIAQSPP---VLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQ 74
           LV+A +++  S     V+DI+G  +  +  Y++ P I    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 75  VPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQA-FSICITSTAWRVGEADPTTGRRF 134
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 135 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 194
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 195 LALDGSAFPFEFVRA 199
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]

BLAST of CmaCh12G010860 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 316.6 bits (810), Expect = 3.0e-83
Identity = 153/200 (76.50%), Postives = 169/200 (84.50%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           M   KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-80
Identity = 149/200 (74.50%), Postives = 162/200 (81.00%), Query Frame = 1


Query: 1   MMLCKSLVAFSYLCLVMAASSIA---QSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           MML KSL  FS LC  MA  S A     PPVLD EGRPL+ + EYFIKPAITDVAGNLTL
Sbjct: 2   MMLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQE GF V+  PY   ED IREGRDLK VFQ F+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGEADP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGILI
Sbjct: 122 KVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 300.1 bits (767), Expect = 2.9e-78
Identity = 147/201 (73.13%), Postives = 161/201 (80.10%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIA---QSPPVLDIEGRPLEINVEYFIKPAITDVAGNLT 60
           M   KSL + FS LC  MA  S A     PPVLD EGRPL+ + EYFIKPAITDVAGNLT
Sbjct: 2   MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTA 120
           L+TRKGDQCPF+VGQVPL SQE GF V+  PY   ED IREGRDLK VFQ F+ICIT T 
Sbjct: 62  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGIL 180
           W+VGEADP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGIL
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 181

Query: 181 IEKGLRLLALDGSAFPFEFVR 198
           IEKG+R LALDG AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of CmaCh12G010860 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-58
Identity = 117/198 (59.09%), Postives = 135/198 (68.18%), Query Frame = 1


Query: 1   MMLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTR 60
           MML KSL  F Y CL MA +S AQ PPVLD  G+PL+  VEY+I PAITDV GNLTL +R
Sbjct: 2   MMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSR 61

Query: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVG 120
               CP  VGQ P+ S  IG  VTF P     D+I EG  L +VFQA S C+TST WRV 
Sbjct: 62  SNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVD 121

Query: 121 EADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180
             +  TGRRFV  GD+D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G
Sbjct: 122 ATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENG 181

Query: 181 LRLLALDGSAFPFEFVRA 199
           +RL+ALDG AFPFEF++A
Sbjct: 182 VRLVALDGDAFPFEFIKA 195

[Close]

BLAST of CmaCh12G010860 vs. NCBI nr
Match: gi|242346705|gb|ACS92521.1| (Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides])

HSP 1 Score: 222.2 bits (565), Expect = 7.8e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

The following BLAST results are available for this feature:

BLAST of CmaCh12G010860 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	5.8e-18	34.98	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
IAAS_ORYSJ	9.6e-13	36.50	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
IAAS_WHEAT	2.8e-12	33.33	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1	[more]
ASP_THECC	5.0e-09	29.27	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]
PIP21_PEA	1.4e-08	28.85	Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV...	[more]

BLAST of CmaCh12G010860 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4_CUCSA	2.1e-83	76.50	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1	[more]
D1KFM4_9ROSI	5.4e-55	61.03	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=...	[more]
G0Z2L7_POPNI	5.4e-55	60.51	Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1	[more]
D1KFM3_9ROSI	5.4e-55	61.03	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides...	[more]
B9N8J2_POPTR	9.3e-55	61.03	Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPT...	[more]

BLAST of CmaCh12G010860 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G73260.1	2.4e-17	31.28	kunitz trypsin inhibitor 1	[more]

BLAST of CmaCh12G010860 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442849\|ref\|XP_004139193.1\|	3.0e-83	76.50	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus]	[more]
gi\|659070428\|ref\|XP_008455022.1\|	1.1e-80	74.50	PREDICTED: miraculin-like isoform X2 [Cucumis melo]	[more]
gi\|659070426\|ref\|XP_008455014.1\|	2.9e-78	73.13	PREDICTED: miraculin-like isoform X1 [Cucumis melo]	[more]
gi\|449442847\|ref\|XP_004139192.1\|	4.4e-58	59.09	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|242346705\|gb\|ACS92521.1\|	7.8e-55	61.03	Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G010860.1	CmaCh12G010860.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 28..196 score: 1.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 27..198 score: 1.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 28..44 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 26..197 score: 6.35
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 25..198 score: 6.8
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 5..198 score: 3.8
None	No IPR available	PANTHER	PTHR33107:SF4	SUBFAMILY NOT NAMED	coord: 5..198 score: 3.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh12G010860	Cucsa.091720	Cucumber (Gy14) v1	cgycmaB0179
CmaCh12G010860	Cla019870	Watermelon (97103) v1	cmawmB170
CmaCh12G010860	Csa2G021000	Cucumber (Chinese Long) v2	cmacuB173
CmaCh12G010860	MELO3C004296	Melon (DHL92) v3.5.1	cmameB172
CmaCh12G010860	ClCG02G015990	Watermelon (Charleston Gray)	cmawcgB156
CmaCh12G010860	CSPI02G03680	Wild cucumber (PI 183967)	cmacpiB174
CmaCh12G010860	CmoCh12G011020	Cucurbita moschata (Rifu)	cmacmoB177
CmaCh12G010860	Lsi11G005210	Bottle gourd (USVL1VR-Ls)	cmalsiB159
CmaCh12G010860	Cp4.1LG11g09390	Cucurbita pepo (Zucchini)	cmacpeB171
CmaCh12G010860	Cp4.1LG07g09060	Cucurbita pepo (Zucchini)	cmacpeB203
CmaCh12G010860	MELO3C000887.2	Melon (DHL92) v3.6.1	cmamedB187
CmaCh12G010860	CsaV3_2G005010	Cucumber (Chinese Long) v3	cmacucB0202
CmaCh12G010860	Cla97C02G041760	Watermelon (97103) v2	cmawmbB175
CmaCh12G010860	Bhi05G001663	Wax gourd	cmawgoB0193
CmaCh12G010860	CsGy2G003790	Cucumber (Gy14) v2	cgybcmaB178
CmaCh12G010860	Carg12887	Silver-seed gourd	carcmaB1241
CmaCh12G010860	Carg17559	Silver-seed gourd	carcmaB1000

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh12G010860	CmaCh05G011550	Cucurbita maxima (Rimu)	cmacmaB190

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh12G010860	Wax gourd	cmawgoB0236
CmaCh12G010860	Cucurbita moschata (Rifu)	cmacmoB190
CmaCh12G010860	Cucumber (Gy14) v2	cgybcmaB054