Carg12887 (gene) Silver-seed gourd

Overview
NameCarg12887
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionKunitz-type trypsin inhibitor
LocationCucurbita_argyrosperma_scaffold_043 : 985357 .. 985893 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTCAACCTCTACGACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACTGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCACTCTACGTTGGTCAAGAACCAATTGCTTCGACAAATATCGGTCTTCCGGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGTGTTTCAAGCGTCGTCAACGTGCATAACATCAACGCAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTTGGGAACGATAACGGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGAGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

mRNA sequence

ATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTCAACCTCTACGACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACTGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCACTCTACGTTGGTCAAGAACCAATTGCTTCGACAAATATCGGTCTTCCGGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGTGTTTCAAGCGTCGTCAACGTGCATAACATCAACGCAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTTGGGAACGATAACGGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGAGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Coding sequence (CDS)

ATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTCAACCTCTACGACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACTGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCACTCTACGTTGGTCAAGAACCAATTGCTTCGACAAATATCGGTCTTCCGGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGTGTTTCAAGCGTCGTCAACGTGCATAACATCAACGCAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTTGGGAACGATAACGGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGAGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Protein sequence

MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Homology
[Close]
BLAST of Carg12887 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 367.9 bits (943), Expect = 2.0e-98
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0 

Query: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60
           MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS
Sbjct: 16  MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 75

Query: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120
           TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND
Sbjct: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 135

Query: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Sbjct: 136 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 193

[Close]
BLAST of Carg12887 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.1 bits (928), Expect = 1.1e-96
Identity = 174/178 (97.75%), Postives = 176/178 (98.88%), Query Frame = 0 

Query: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60
           MAITSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEP+AS
Sbjct: 16  MAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVAS 75

Query: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120
           TNIGLPVTFTPIVAGEDIIEESMGFNV FQASSTCITSTQWRVDEMESATGRRFVGVGND
Sbjct: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135

Query: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           N PTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Sbjct: 136 NSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 193

[Close]
BLAST of Carg12887 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 361.7 bits (927), Expect = 1.4e-96
Identity = 173/178 (97.19%), Postives = 177/178 (99.44%), Query Frame = 0 

Query: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60
           +AITSTAQLPPVLDTDGQPLRRGVEYYIKPAIT+VAGNLTLK+RSNAPCPLYVGQEP+AS
Sbjct: 16  LAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSNAPCPLYVGQEPVAS 75

Query: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120
           TNIGLPVTFTPIVAGEDIIEESMGFNV FQASSTCITSTQWRVDEMESATGRRFVGVGND
Sbjct: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135

Query: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Sbjct: 136 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 193

[Close]
BLAST of Carg12887 vs. NCBI nr
Match: XP_022150789.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 299.7 bits (766), Expect = 6.7e-78
Identity = 148/180 (82.22%), Postives = 158/180 (87.78%), Query Frame = 0 

Query: 1   MAI-TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIA 60
           MAI +STAQ PPVLDT+GQ LRRG+EYYIKPAITDVAGNLTLKSRS APCPLYVGQEP+A
Sbjct: 18  MAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRAPCPLYVGQEPVA 77

Query: 61  STNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGN 120
           S  IGLPV FTP  A EDII E M F VVFQASSTCI  TQWRV+E E+ATGRR VG G+
Sbjct: 78  SMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAATGRRLVGTGD 137

Query: 121 DNGPTGIFRIDRNN-GVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           DNGPTG+FRIDR+N GVYNI WCPEM GRPRCGSAGILVEDGVRLLALDG+AFPFEFVKA
Sbjct: 138 DNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGSAFPFEFVKA 197

[Close]
BLAST of Carg12887 vs. NCBI nr
Match: XP_004139192.1 (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 299.3 bits (765), Expect = 8.8e-78
Identity = 139/178 (78.09%), Postives = 156/178 (87.64%), Query Frame = 0 

Query: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60
           MAITSTAQLPPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRSNAPCPL+VGQEP+ S
Sbjct: 18  MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77

Query: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120
           TNIGLPVTF P   G DII+E    N+VFQA STC+TSTQWRVD  ES TGRRFVG+G++
Sbjct: 78  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 137

Query: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           +GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+ALDG+AFPFEF+KA
Sbjct: 138 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 195

[Close]
BLAST of Carg12887 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 51/174 (29.31%), Postives = 84/174 (48.28%), Query Frame = 0 

Query: 12  VLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIASTN---IGLPVT 71
           VLD  G P++  V+YYI PA     G L   +R+ +   L +  + + S++    GLPVT
Sbjct: 32  VLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSSSPFVSGLPVT 91

Query: 72  FTPIVAGEDIIEESMGFNVVFQASS-TCITSTQWRVDEMESATGRRFVGVGNDNGP-TGI 131
           F+P+      ++ S   N+ F ++   C  S  WR+D       + FV +G   G     
Sbjct: 92  FSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQLR-KSFVSIGGQKGKGNSW 151

Query: 132 FRIDRNNGVYNIVWCPEMVGRPRCGSAGILVED-GVRLLALD-GNAFPFEFVKA 179
           F+I  +   Y +++CP +     C +  + ++D GVR L L    +F  +F KA
Sbjct: 152 FQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLSTDQSFVVKFQKA 203

[Close]
BLAST of Carg12887 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 100.1 bits (248), Expect = 2.6e-20
Identity = 65/183 (35.52%), Postives = 91/183 (49.73%), Query Frame = 0 

Query: 11  PVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS---RSNAPCPLYVGQEPIASTNIGLPV 70
           PVLD DG+ LR G  YYI P + D  G LT+ +        CP  V Q      +   P+
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR-KEVDHDRPL 94

Query: 71  TFTPIVAGEDIIEESMGFNVVFQASSTC--ITSTQWRVDEMESATGRRFVGVGNDNGPTG 130
            F P    ED++  S   N+ F A   C   +ST WR+D+ + +TG+ FV +G   G  G
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 131 ------IFRIDR--NNGVYNIVWCPEMVG--RPRCGSAGILVED-GVRLLALDGNAFPFE 178
                  F+I+    +G Y +V+CP + G  + +CG  GI ++  G R LAL    F FE
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFE 214

[Close]
BLAST of Carg12887 vs. Swiss-Prot
Match: sp|P07596|IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 55/153 (35.95%), Postives = 76/153 (49.67%), Query Frame = 0 

Query: 7   AQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIASTNIGLP 66
           A  PPV DTDG  LR    YY+  A     G LT+       CPL+V Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDPNGQHD-GFP 82

Query: 67  VTFTP--IVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVG--NDNG 126
           V  TP  +   + II  S    + F+A +TC+ ST+W +D  E A GRR V  G   D  
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDS-ELAAGRRHVITGPVKDPS 142

Query: 127 PTG---IFRIDRNNGV----YNIV----WCPEM 145
           P+G    FRI++ +G     Y ++    WC ++
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSCGDWCQDL 173

[Close]
BLAST of Carg12887 vs. Swiss-Prot
Match: sp|P16347|IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.6e-14
Identity = 53/143 (37.06%), Postives = 71/143 (49.65%), Query Frame = 0 

Query: 10  PPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIASTNIGLPVTF 69
           PPV DTDG  LR    YY+ PA     G LT+       CPL+V QE     + GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRD-GLPVRI 62

Query: 70  TPI--VAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVG--NDNGPTG 129
            P      + II  S    + F+A +TC+ ST+W +D  E  +GRR V  G   D  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDS-ELVSGRRHVITGPVRDPSPSG 122

Query: 130 ---IFRIDRNNGV----YNIVWC 142
               FRI++ +G     Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

[Close]
BLAST of Carg12887 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.0e-13
Identity = 59/186 (31.72%), Postives = 88/186 (47.31%), Query Frame = 0 

Query: 11  PVLDTDGQPLRRGVEYYIKPAITDV-AGNLTLKSRSNAPCPLYVGQEPIASTNIGLPVTF 70
           PVLDTDG  L+ GV+YY+  +I+    G L L   +   CP  V Q      N G PV F
Sbjct: 30  PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDN-GTPVIF 89

Query: 71  TPIVAGEDIIEESMGFNVVFQ--ASSTCITSTQWRVDEMESATGRRFV---GVGNDNGPT 130
           +   + +D++  S   N+ F       C TST WR+D  +++ G+ +V   GV  + GP 
Sbjct: 90  SNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPN 149

Query: 131 GI---FRIDRNNGV-YNIVWCPEMVGRPRCGSAGILV--------EDGVRLLALDGNAFP 179
            +   F+I++   + Y   +CP +     C S   L         +DG   LAL  N + 
Sbjct: 150 TLCSWFKIEKAGVLGYKFRFCPSV-----CDSCTTLCSDIGRHSDDDGQIRLALSDNEWA 209

[Close]
BLAST of Carg12887 vs. Swiss-Prot
Match: sp|P25272|KTI1_SOYBN (Kunitz-type trypsin inhibitor KTI1 OS=Glycine max OX=3847 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-11
Identity = 50/170 (29.41%), Postives = 75/170 (44.12%), Query Frame = 0 

Query: 4   TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIASTNI 63
           ++TAQ   VLDTD  PL+ G  YY+ P +    G + + S     CPL V Q P    + 
Sbjct: 22  SATAQF--VLDTDDDPLQNGGTYYMLPVMRGKGGGIEVDSTGKEICPLTVVQSP-NELDK 81

Query: 64  GLPVTFTPIVAGEDIIEE---SMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 123
           G+ + FT  +    I E    S+ F      +      T+W + E E     +       
Sbjct: 82  GIGLVFTSPLHALFIAERYPLSIKFGSFAVITLCAGMPTEWAIVEREGLQAVKLAARDTV 141

Query: 124 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILV-EDGVRLLALDGN 170
           +G   I R+ R    Y +V+CP+     +C   GI + +DG+R L L  N
Sbjct: 142 DGWFNIERVSREYNDYKLVFCPQQAEDNKCEDIGIQIDDDGIRRLVLSKN 188

[Close]
BLAST of Carg12887 vs. TrEMBL
Match: tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.8e-78
Identity = 139/178 (78.09%), Postives = 156/178 (87.64%), Query Frame = 0 

Query: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60
           MAITSTAQLPPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRSNAPCPL+VGQEP+ S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120
           TNIGLPVTF P   G DII+E    N+VFQA STC+TSTQWRVD  ES TGRRFVG+G++
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 179
           +GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]
BLAST of Carg12887 vs. TrEMBL
Match: tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.5e-57
Identity = 117/183 (63.93%), Postives = 134/183 (73.22%), Query Frame = 0 

Query: 1   MAITSTAQL--PPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPI 60
           MAI STAQ   PPVLDT+G+PL+R VEY+IKPAITDVAGNLTL +R    CP YVGQ P+
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  ASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVG 120
            S  IG  VT TP + G+D I E      VFQA + CIT TQW+V E +  +GRRFV +G
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 NDNGPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVEDGVRLLALDGNAFPFE 178
           NDN  TG FRID++N GVYNI WCP  V   GRPRCGSAGIL+E GVR LALDG AFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

[Close]
BLAST of Carg12887 vs. TrEMBL
Match: tr|A0A1S3C0M7|A0A1S3C0M7_CUCME (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=4 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.1e-52
Identity = 111/184 (60.33%), Postives = 127/184 (69.02%), Query Frame = 0 

Query: 1   MAITSTA---QLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEP 60
           MAI STA     PPVLDT+G+PL+R  EY+IKPAITDVAGNLTL +R    CP YVGQ P
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  IASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGV 120
           + S   G  V+  P   GED I E      VFQ  + CIT TQW+V E +  TGRRFV +
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNGPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVEDGVRLLALDGNAFPF 178
           G DN  TG FRID+++ GVYNI WCP  V   GRPRCGSAGIL+E GVR LALDG AFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

[Close]
BLAST of Carg12887 vs. TrEMBL
Match: tr|B9N8J2|B9N8J2_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G000400v3 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.4e-49
Identity = 110/188 (58.51%), Postives = 120/188 (63.83%), Query Frame = 0 

Query: 1   MAITSTA-----QLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQ 60
           MAI S A     Q PPVLDT GQPL  GVEYYI P ITDVAG LTL +R+   CP YVGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  EPIASTN-IGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRF 120
           EP+AS    G  V FTP  +GE II ES   +V FQAS+ CI ST WRV E +  TGRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VGVGNDNGPTGIFRIDRNNGVYNIVWCP----EMVGRPRCGSAGILVEDGVRLLALDGNA 179
           +    D      FRID N GVYN  WCP        RPRCGSAGIL+ED  RLLALDG A
Sbjct: 135 IVTAGDK---SYFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPA 194

[Close]
BLAST of Carg12887 vs. TrEMBL
Match: tr|E9NH21|E9NH21_POPNI (Kunitz-type trypsin inhibitor OS=Populus nigra OX=3691 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.4e-49
Identity = 110/188 (58.51%), Postives = 120/188 (63.83%), Query Frame = 0 

Query: 1   MAITSTA-----QLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQ 60
           MAI S A     Q PPVLDT GQPL  GVEYYI P ITDVAG LTL +R+   CP YVGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  EPIASTN-IGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRF 120
           EP+AS    G  V FTP  +GE II ES   +V FQAS+ CI ST WRV E +  TGRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VGVGNDNGPTGIFRIDRNNGVYNIVWCP----EMVGRPRCGSAGILVEDGVRLLALDGNA 179
           +    D      FRID N GVYN  WCP        RPRCGSAGIL+ED  RLLALDG A
Sbjct: 135 IVTAGDK---SYFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPA 194

The following BLAST results are available for this feature:
BLAST of Carg12887 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022946294.12.0e-98100.00miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo... [more]
XP_023545477.11.1e-9697.75miraculin-like [Cucurbita pepo subsp. pepo][more]
XP_022999136.11.4e-9697.19miraculin-like [Cucurbita maxima][more]
XP_022150789.16.7e-7882.22miraculin-like [Momordica charantia][more]
XP_004139192.18.8e-7878.09PREDICTED: miraculin-like [Cucumis sativus][more]
BLAST of Carg12887 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73325.12.7e-1229.31Kunitz family trypsin and protease inhibitor protein[more]
BLAST of Carg12887 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P13087|MIRA_SYNDU2.6e-2035.52Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3[more]
sp|P07596|IAAS_HORVU1.5e-1535.95Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2[more]
sp|P16347|IAAS_WHEAT1.6e-1437.06Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ... [more]
sp|P32765|ASP_THECC4.0e-1331.7221 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1[more]
sp|P25272|KTI1_SOYBN1.3e-1129.41Kunitz-type trypsin inhibitor KTI1 OS=Glycine max OX=3847 GN=KTI1 PE=2 SV=1[more]
BLAST of Carg12887 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA5.8e-7878.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1[more]
tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA2.5e-5763.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1[more]
tr|A0A1S3C0M7|A0A1S3C0M7_CUCME1.1e-5260.33endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|B9N8J2|B9N8J2_POPTR7.4e-4958.51Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_004G000400v3 PE=... [more]
tr|E9NH21|E9NH21_POPNI7.4e-4958.51Kunitz-type trypsin inhibitor OS=Populus nigra OX=3691 PE=2 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12887-RACarg12887-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 11..178
e-value: 1.1E-30
score: 117.9
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 12..177
e-value: 9.3E-42
score: 142.9
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..177
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 12..28
IPR002160Proteinase inhibitor I3, Kunitz legumeCDDcd00178STIcoord: 11..177
e-value: 3.04224E-39
score: 132.466
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 9..178
e-value: 1.1E-44
score: 154.3
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..177
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILYSSF50386STI-likecoord: 10..178