CmoCh12G011020 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh12G011020
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionKunitz trypsin inhibitor
LocationCmo_Chr12 : 10081814 .. 10082513 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATTAATGATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTATTATTACATCACAATCGAACTCAAAAACTTTTGATTAGTGATACCATTAGAAAACTCTTGATTATGTTACGAATTCTTAACCATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGA

mRNA sequence

CAAATTAATGATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGA

Coding sequence (CDS)

ATGATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA
Homology
[Close]
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 90.9 bits (224), Expect = 1.7e-17
Identity = 64/182 (35.16%), Postives = 90/182 (49.45%), Query Frame = 1 

Query: 27  PVLDTEGRPLQINVEYFIKPAVTDVAGNLTL--LTRKGD-QCPFHVGQVPLRSQEIGFGV 86
           PVLD +G  L+    Y+I P + D  G LT+   T  G   CP  V Q   +  +    +
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR-KEVDHDRPL 94

Query: 87  TFTPYLPGEDTIREGRDLKVVFQAFSIC--ITSTAWRVGETDPTTGRRFVETG------D 146
            F P  P ED +R   DL + F AF  C   +ST WR+ + D +TG+ FV  G       
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 147 DDTATGYFRIDR-DNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRLLALDGSAFPF 196
            +T + +F+I+     G Y + +CP+ V    + +CG  GI I +KG R LAL    F F
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAF 214

[Close]
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.1e-14
Identity = 52/137 (37.96%), Postives = 71/137 (51.82%), Query Frame = 1 

Query: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 61
           ++  SL+A S+ C        A  PPV DTEG  L  +  Y++ PA     G LT+  R 
Sbjct: 8   LILLSLLAISFSCS-------AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 67

Query: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPY----LPGEDTIREGRDLKVVFQAFSICITSTAW 121
              CP  V Q     ++ GF V FTP+     P + TIR   D+++ F A +IC+ ST W
Sbjct: 68  LP-CPLLVAQETDERRK-GFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 127

Query: 122 RVGETDPTTGRRFVETG 135
            VG+ +P TG R V TG
Sbjct: 128 HVGD-EPLTGARRVVTG 134

[Close]
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 43/129 (33.33%), Postives = 68/129 (52.71%), Query Frame = 1 

Query: 26  PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTF 85
           PPV DT+G  L+ +  Y++ PA     G LT+    G +CP  V Q     Q  G  V  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQ-EADGQRDGLPVRI 62

Query: 86  TPY--LPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD--DDTATG 145
            P+   P +  IR   D+++ F+A++ C+ ST W + +++  +GRR V TG   D + +G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSPSG 122

Query: 146 ---YFRIDR 148
               FRI++
Sbjct: 123 RENAFRIEK 129

[Close]
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.5e-10
Identity = 50/164 (30.49%), Postives = 77/164 (46.95%), Query Frame = 1 

Query: 7   LVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDV-AGNLTLLTRKGDQC 66
           L AF+       V++ A SP VLDT+G  LQ  V+Y++  +++    G L L    G  C
Sbjct: 11  LFAFTSKSYFFGVANAANSP-VLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 70

Query: 67  PFHVGQVPLRSQ-EIGFGVTFTPYLPGEDTIREGRDLKVVFQAF--SICITSTAWRVGET 126
           P  V  V  RS  + G  V F+     +D +R   D+ + F      +C TST WR+   
Sbjct: 71  PEIV--VQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNY 130

Query: 127 DPTTGRRFVETG------DDDTATGYFRIDRDNFGVYNIGWCPS 161
           D + G+ +V T         +T   +F+I++     Y   +CPS
Sbjct: 131 DNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 4.6e-07
Identity = 57/208 (27.40%), Postives = 85/208 (40.87%), Query Frame = 1 

Query: 3   LCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKG 62
           L  S   F ++  +    S      VLD  G P+   V+YFI PA+   AG    L R G
Sbjct: 7   LTLSFFLFVFITNLSLAFSNEDVEQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTG 66

Query: 63  D-QCPFHVGQVPLRSQEIGFGVTFTPYLP--GEDTIREGRDLKVVFQAFSICITSTAWRV 122
           D  CP  V Q     QE+  G+     +P      I  G  +++ +     C  S+ W V
Sbjct: 67  DLTCPVTVLQ---DRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKWLV 126

Query: 123 GETDPTTGRRFVETGDDD------TATGYFRIDRDNFGV-YNIGWCPSDVPIRGRPRCGR 182
              D    +  V  G  +      T +G F+I++   G  Y +G+C     ++G P C  
Sbjct: 127 -FVDNVIQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFC-----VKGSPTCLD 186

Query: 183 AGIL--IEKGLRLLALDGSAFPFEFVRA 199
            G     E G RL   +  +F   F++A
Sbjct: 187 VGRFDNDEDGRRLNLTEHESFQVVFIQA 205

[Close]
BLAST of CmoCh12G011020 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 3.5e-86
Identity = 156/200 (78.00%), Postives = 173/200 (86.50%), Query Frame = 1 

Query: 1   MMLCKSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTL 60
           M   KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]
BLAST of CmoCh12G011020 vs. TrEMBL
Match: A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.9e-56
Identity = 110/182 (60.44%), Postives = 128/182 (70.33%), Query Frame = 1 

Query: 17  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 76
           MA++S AQ PPVLDT G+PLQ  VEY+I PA+TDV GNLTL +R    CP  VGQ P+ S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 77  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 136
             IG  VTF P   G D I EG  L +VFQA S C+TST WRV  T+  TGRRFV  GD+
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196
           D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G+RL+ALDG AFPFEF+
Sbjct: 121 DGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFEFI 178

Query: 197 RA 199
           +A
Sbjct: 181 KA 178

[Close]
BLAST of CmoCh12G011020 vs. TrEMBL
Match: D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 3.8e-56
Identity = 119/195 (61.03%), Postives = 136/195 (69.74%), Query Frame = 1 

Query: 11  SYLCLVMAVSSIAQ-----SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQC 70
           S++ L MA+ S+AQ     SPPVLDT G+PL+  VEY+I P +TDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDP 130
           PF+VGQ PL S E  G  V FTPY  GE  IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]
BLAST of CmoCh12G011020 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 3.8e-56
Identity = 119/195 (61.03%), Postives = 136/195 (69.74%), Query Frame = 1 

Query: 11  SYLCLVMAVSSIAQ-----SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQC 70
           S++ L MA+ S+AQ     SPPVLDT G+PL+  VEY+I P +TDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDP 130
           PF+VGQ PL S E  G  V FTPY  GE  IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]
BLAST of CmoCh12G011020 vs. TrEMBL
Match: G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 3.8e-56
Identity = 118/195 (60.51%), Postives = 136/195 (69.74%), Query Frame = 1 

Query: 11  SYLCLVMAVSSIAQ-----SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQC 70
           S++ L MA+ S+AQ     SPPVLDT G+PL+  VEY+I P +TD+AG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQEI-GFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDP 130
           PF+VGQ PL S E  G  V FTPY  GE  IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]
BLAST of CmoCh12G011020 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-16
Identity = 59/195 (30.26%), Postives = 90/195 (46.15%), Query Frame = 1 

Query: 15  LVMAVSSIAQSPP---VLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQ 74
           LV+A++++  S     V+D +G  +  +  Y++ P +    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 75  VPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQA-FSICITSTAWRVGETDPTTGRRF 134
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 135 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 194
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 195 LALDGSAFPFEFVRA 199
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]
BLAST of CmoCh12G011020 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 325.9 bits (834), Expect = 5.0e-86
Identity = 156/200 (78.00%), Postives = 173/200 (86.50%), Query Frame = 1 

Query: 1   MMLCKSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTL 60
           M   KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]
BLAST of CmoCh12G011020 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 315.8 bits (808), Expect = 5.2e-83
Identity = 151/200 (75.50%), Postives = 165/200 (82.50%), Query Frame = 1 

Query: 1   MMLCKSLVAFSYLCLVMAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTL 60
           MML KSL  FS LC  MA+ S A     PPVLDTEGRPLQ + EYFIKPA+TDVAGNLTL
Sbjct: 2   MMLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQE GF V+  PY  GEDTIREGRDLK VFQ F+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQW 121

Query: 121 RVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGILI
Sbjct: 122 KVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]
BLAST of CmoCh12G011020 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 307.8 bits (787), Expect = 1.4e-80
Identity = 149/201 (74.13%), Postives = 164/201 (81.59%), Query Frame = 1 

Query: 1   MMLCKSLVA-FSYLCLVMAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLT 60
           M   KSL + FS LC  MA+ S A     PPVLDTEGRPLQ + EYFIKPA+TDVAGNLT
Sbjct: 2   MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTA 120
           L+TRKGDQCPF+VGQVPL SQE GF V+  PY  GEDTIREGRDLK VFQ F+ICIT T 
Sbjct: 62  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGIL 180
           W+VGE DP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGIL
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 181

Query: 181 IEKGLRLLALDGSAFPFEFVR 198
           IEKG+R LALDG AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 202

[Close]
BLAST of CmoCh12G011020 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-60
Identity = 120/198 (60.61%), Postives = 138/198 (69.70%), Query Frame = 1 

Query: 1   MMLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 60
           MML KSL  F Y CL MA++S AQ PPVLDT G+PLQ  VEY+I PA+TDV GNLTL +R
Sbjct: 2   MMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSR 61

Query: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 120
               CP  VGQ P+ S  IG  VTF P   G D I EG  L +VFQA S C+TST WRV 
Sbjct: 62  SNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVD 121

Query: 121 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180
            T+  TGRRFV  GD+D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G
Sbjct: 122 ATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENG 181

Query: 181 LRLLALDGSAFPFEFVRA 199
           +RL+ALDG AFPFEF++A
Sbjct: 182 VRLVALDGDAFPFEFIKA 195

[Close]
BLAST of CmoCh12G011020 vs. NCBI nr
Match: gi|700205760|gb|KGN60879.1| (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 4.1e-56
Identity = 110/182 (60.44%), Postives = 128/182 (70.33%), Query Frame = 1 

Query: 17  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 76
           MA++S AQ PPVLDT G+PLQ  VEY+I PA+TDV GNLTL +R    CP  VGQ P+ S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 77  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 136
             IG  VTF P   G D I EG  L +VFQA S C+TST WRV  T+  TGRRFV  GD+
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196
           D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G+RL+ALDG AFPFEF+
Sbjct: 121 DGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFEFI 178

Query: 197 RA 199
           +A
Sbjct: 181 KA 178

The following BLAST results are available for this feature:
BLAST of CmoCh12G011020 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU1.7e-1735.16Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
IAAS_ORYSJ5.1e-1437.96Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ... [more]
IAAS_WHEAT1.6e-1233.33Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1[more]
ASP_THECC4.5e-1030.4921 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
PIP21_PEA4.6e-0727.40Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV... [more]
BLAST of CmoCh12G011020 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LGB4_CUCSA3.5e-8678.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1[more]
A0A0A0LIK2_CUCSA2.9e-5660.44Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1[more]
D1KFM4_9ROSI3.8e-5661.03Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=... [more]
D1KFM3_9ROSI3.8e-5661.03Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
G0Z2L7_POPNI3.8e-5660.51Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1[more]
BLAST of CmoCh12G011020 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73260.12.0e-1630.26 kunitz trypsin inhibitor 1[more]
BLAST of CmoCh12G011020 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442849|ref|XP_004139193.1|5.0e-8678.00PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus][more]
gi|659070428|ref|XP_008455022.1|5.2e-8375.50PREDICTED: miraculin-like isoform X2 [Cucumis melo][more]
gi|659070426|ref|XP_008455014.1|1.4e-8074.13PREDICTED: miraculin-like isoform X1 [Cucumis melo][more]
gi|449442847|ref|XP_004139192.1|1.6e-6060.61PREDICTED: miraculin-like [Cucumis sativus][more]
gi|700205760|gb|KGN60879.1|4.1e-5660.44hypothetical protein Csa_2G021500 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002160Prot_inh_Kunz-lg
IPR011065Kunitz_inhibitor_STI-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G011020.1CmoCh12G011020.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 28..196
score: 1.0
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 27..198
score: 8.9
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 28..44
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 26..197
score: 4.86
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 25..198
score: 4.0
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 5..198
score: 2.9
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 5..198
score: 2.9