CsaV3_2G005010 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_2G005010
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Descriptionendogenous alpha-amylase/subtilisin inhibitor-like
Locationchr2 : 2715081 .. 2715786 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTATGTATTAAATCTGTTAGAGTGTTGATATACAACTATTAAATGTGTACCTACACTTCACTCACTATTTAAGCAGATAGATTATGATATATTGAATTCTTAACAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

mRNA sequence

ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Coding sequence (CDS)

ATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGAATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Protein sequence

MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Homology
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BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_004139193.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] >KGN60878.1 hypothetical protein Csa_2G021000 [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 5.9e-115
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

Query: 181 FVRVDTGEELGSILKEKK 199
           FVRVDTGEELGSILKEKK
Sbjct: 199 FVRVDTGEELGSILKEKK 216

[Close]
BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 383.6 bits (984), Expect = 4.0e-103
Identity = 181/198 (91.41%), Postives = 186/198 (93.94%), Query Frame = 0 

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           LLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEK 198
           EFVRVD  EELGSIL+EK
Sbjct: 199 EFVRVDPVEELGSILQEK 216

[Close]
BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 320.5 bits (820), Expect = 4.1e-84
Identity = 147/183 (80.33%), Postives = 162/183 (88.52%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 17  MAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPL 76

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
            SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 77  RSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 136

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           +D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 137 DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 196

Query: 181 FVR 184
           FVR
Sbjct: 197 FVR 197

[Close]
BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318.5 bits (815), Expect = 1.6e-83
Identity = 146/183 (79.78%), Postives = 162/183 (88.52%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPAITDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 17  MAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPL 76

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
            SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 77  RSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 136

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           +++TATGYFRID+ N G+YNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 137 DNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 196

Query: 181 FVR 184
           FVR
Sbjct: 197 FVR 197

[Close]
BLAST of CsaV3_2G005010 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 311.2 bits (796), Expect = 2.5e-81
Identity = 144/183 (78.69%), Postives = 158/183 (86.34%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 17  MAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPL 76

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
            SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 77  RSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETG 136

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           +D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 137 DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 196

Query: 181 FVR 184
           FVR
Sbjct: 197 FVR 197

[Close]
BLAST of CsaV3_2G005010 vs. TAIR10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-16
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0 

Query: 14  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 73
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 74  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 133
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 134 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 189
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

[Close]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match: sp|Q8RXD5|KTI1_ARATH (Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.7e-15
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0 

Query: 14  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 73
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 74  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 133
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 134 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 189
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

[Close]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match: sp|P29421|IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 8.9e-14
Identity = 56/167 (33.53%), Postives = 75/167 (44.91%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           +AI  +     PPV DTEG  L  D  Y++ PA     G LT+  R    CP  V Q   
Sbjct: 14  LAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCPLLVAQ-ET 73

Query: 61  LSQEIGFGVTLTPY----LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRF 120
             +  GF V  TP+       D TIR   D++  F A TIC+  T+W VG+E P +G R 
Sbjct: 74  DERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDE-PLTGARR 133

Query: 121 VKLG-----NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGS 159
           V  G     + +     FR++K   G Y +  C       G  R G+
Sbjct: 134 VVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

[Close]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 7.8e-10
Identity = 43/143 (30.07%), Postives = 66/143 (46.15%), Query Frame = 0 

Query: 13  PVLDTEGRPLQRDVEYFIKPAITDV-AGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTL 72
           PVLDT+G  LQ  V+Y++  +I+    G L L    G  CP  V Q      + G  V  
Sbjct: 30  PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQ-RRSDLDNGTPVIF 89

Query: 73  TPYLEGDDTIREGRD--LKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG------NDN 132
           +     DD +R   D  ++FV     +C T T W++   D  +G+ +V           N
Sbjct: 90  SNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPN 149

Query: 133 TATGYFRIDKSNLGVYNIGWCPS 147
           T   +F+I+K+ +  Y   +CPS
Sbjct: 150 TLCSWFKIEKAGVLGYKFRFCPS 171

[Close]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match: sp|P25272|KTI1_SOYBN (Kunitz-type trypsin inhibitor KTI1 OS=Glycine max OX=3847 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 52/175 (29.71%), Postives = 78/175 (44.57%), Query Frame = 0 

Query: 5   STAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQE 64
           +TAQF    VLDT+  PLQ    Y++ P +    G + + +   + CP  V Q P    +
Sbjct: 23  ATAQF----VLDTDDDPLQNGGTYYMLPVMRGKGGGIEVDSTGKEICPLTVVQSP-NELD 82

Query: 65  IGFGVTLTPYLEGDDTIREGRDLKFVFQAF---TICI-TGTQWKVGEEDPKSGRRFVKLG 124
            G G+  T  L     I E   L   F +F   T+C    T+W + E   + G + VKL 
Sbjct: 83  KGIGLVFTSPLHA-LFIAERYPLSIKFGSFAVITLCAGMPTEWAIVE---REGLQAVKLA 142

Query: 125 NDNTATGYFRIDK--SNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLAL 173
             +T  G+F I++       Y + +CP         +C   GI I + G+R L L
Sbjct: 143 ARDTVDGWFNIERVSREYNDYKLVFCPQQAEDN---KCEDIGIQIDDDGIRRLVL 185

[Close]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match: sp|P58514|SPI1_SOLTU (Serine protease inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 4.3e-08
Identity = 42/182 (23.08%), Postives = 72/182 (39.56%), Query Frame = 0 

Query: 4   PSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQ 63
           P     D  PVLD  G+ L   + Y I        G    + +  +        V   + 
Sbjct: 26  PINLPSDATPVLDVTGKELDSRLSYRIISTFWGALGGDVYLGKSPNSDAPCANGVFRYNS 85

Query: 64  EIGFGVTLTPYLEGDDTIREG------RDLKFVFQAFTICITGTQWKVGEEDPKSGRRFV 123
           ++G   T   ++       +G       +++F      +C++ T WKVG+ D   G   +
Sbjct: 86  DVGPSGTPVRFIGSSSHFGQGIFENELLNIQFAISTSKLCVSYTIWKVGDYDASLGTMLL 145

Query: 124 KLGN--DNTATGYFRIDKSNLGVYNIGWCP----SDVPFKGRPR-CGSAGILIEKGVRFL 173
           + G       + +F+I KS+   YN+ +CP       PF    + C   G++ + G R L
Sbjct: 146 ETGGTIGQADSSWFKIVKSSQLGYNLLYCPVTSTMSCPFSSDDQFCLKVGVVHQNGKRRL 205

[Close]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match: tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.9e-115
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0 

Query: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

Query: 181 FVRVDTGEELGSILKEKK 199
           FVRVDTGEELGSILKEKK
Sbjct: 199 FVRVDTGEELGSILKEKK 216

[Close]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match: tr|A0A1S3C0M7|A0A1S3C0M7_CUCME (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.6e-103
Identity = 181/198 (91.41%), Postives = 186/198 (93.94%), Query Frame = 0 

Query: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120
           LLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDGPAFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

Query: 181 EFVRVDTGEELGSILKEK 198
           EFVRVD  EELGSIL+EK
Sbjct: 199 EFVRVDPVEELGSILQEK 216

[Close]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match: tr|D1KFM3|D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides OX=3695 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.0e-54
Identity = 113/188 (60.11%), Postives = 127/188 (67.55%), Query Frame = 0 

Query: 1   MAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQ 60
           MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +CPFYVGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  VPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRF 120
            PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEEDP++GRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVRFLALDGP 180
           +    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R LALDGP
Sbjct: 135 IVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGP 194

Query: 181 AFPFEFVR 184
           AFPF F R
Sbjct: 195 AFPFVFTR 198

[Close]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match: tr|D1KFM4|D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra OX=293756 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.0e-54
Identity = 113/188 (60.11%), Postives = 127/188 (67.55%), Query Frame = 0 

Query: 1   MAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQ 60
           MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +CPFYVGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  VPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRF 120
            PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEEDP++GRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVRFLALDGP 180
           +    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R LALDGP
Sbjct: 135 IVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGP 194

Query: 181 AFPFEFVR 184
           AFPF F R
Sbjct: 195 AFPFVFTR 198

[Close]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match: tr|G0Z2L7|G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 6.5e-54
Identity = 112/188 (59.57%), Postives = 127/188 (67.55%), Query Frame = 0 

Query: 1   MAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQ 60
           MAI S AQ      PPVLDT G+PL+  VEY+I P ITD+AG LTL+ R G +CPFYVGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  VPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRF 120
            PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEEDP++GRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVRFLALDGP 180
           +    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R LALDGP
Sbjct: 135 IVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRLLALDGP 194

Query: 181 AFPFEFVR 184
           AFPF F R
Sbjct: 195 AFPFVFTR 198

The following BLAST results are available for this feature:
BLAST of CsaV3_2G005010 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004139193.15.9e-115100.00PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] ... [more]
XP_008455014.14.0e-10391.41PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo][more]
XP_022938134.14.1e-8480.33alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata][more]
XP_023521354.11.6e-8379.78alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381... [more]
XP_022965952.12.5e-8178.69kunitz trypsin inhibitor 1-like [Cucurbita maxima][more]
BLAST of CsaV3_2G005010 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73260.12.6e-1630.60kunitz trypsin inhibitor 1[more]
BLAST of CsaV3_2G005010 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8RXD5|KTI1_ARATH4.7e-1530.60Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1[more]
sp|P29421|IAAS_ORYSJ8.9e-1433.53Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R... [more]
sp|P32765|ASP_THECC7.8e-1030.0721 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1[more]
sp|P25272|KTI1_SOYBN1.1e-0829.71Kunitz-type trypsin inhibitor KTI1 OS=Glycine max OX=3847 GN=KTI1 PE=2 SV=1[more]
sp|P58514|SPI1_SOLTU4.3e-0823.08Serine protease inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2[more]
BLAST of CsaV3_2G005010 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA3.9e-115100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1[more]
tr|A0A1S3C0M7|A0A1S3C0M7_CUCME2.6e-10391.41endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|D1KFM3|D1KFM3_9ROSI5.0e-5460.11Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
tr|D1KFM4|D1KFM4_9ROSI5.0e-5460.11Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra OX=... [more]
tr|G0Z2L7|G0Z2L7_POPNI6.5e-5459.57Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_2G005010.1CsaV3_2G005010.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 13..185
e-value: 8.2E-27
score: 105.1
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 14..182
e-value: 1.2E-35
score: 122.9
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..183
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 14..30
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 11..186
e-value: 3.2E-38
score: 133.1
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..183
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILYSSF50386STI-likecoord: 11..185