CSPI02G03680 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G03680
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Kunitz-type trypsin inhibitor
Location	Chr2 : 2582399 .. 2583631 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAAATGAAGAAATAGTAATTATTGTGATAAAGCTAGACTTTGGTCATTTTCATATCCTCCATGTTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCATTTTTCAAACAAAACTAAATCACTCTTGTTGAACAATCAAAAGATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTATGTATTAAATCTATTACTAAACTAAATTGATTCAATAAGTTATGTGATATCTGTTAGAGTGTTGATATACAACTATTAAATGTGTACCTACACTCACTCACTATTTAAGCAGATAGATTATGATATATTGAATTCTTAACAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAACTAAATAAAACTTGATGTAAGGATTTACTTTTATGTGTGTTCATTGGGGTGTTATATATGATTAATATATATAATGTGATTTGGTAGGTGGATTGGTGTTAAGTAAAAAAGAAAATTAGAGAAATAACAAGTAGAGAAAATAAATGAAATTGTCAACAAATGCTCCTAAAAATCATATAATTGATAGAGACAAATAGCAAATAGTCAAGCTTACCTCCAATGAGAGAAAATTTGTCATAGG

mRNA sequence

ATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Coding sequence (CDS)

ATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Homology

[Close]

BLAST of CSPI02G03680 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.3e-14
Identity = 46/131 (35.11%), Postives = 67/131 (51.15%), Query Frame = 1


Query: 28  DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGV 87
           D PPV DT+G  L+ D  Y++ PA     G LT+    G +CP +V Q     Q  G  V
Sbjct: 1   DPPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQ-EADGQRDGLPV 60

Query: 88  TLTPY--LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG--NDNTA 147
            + P+     D  IR   D++  F+A+T C+  T+W + + +  SGRR V  G   D + 
Sbjct: 61  RIAPHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSP 120

Query: 148 TG---YFRIDK 152
           +G    FRI+K
Sbjct: 121 SGRENAFRIEK 129

[Close]

BLAST of CSPI02G03680 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 9.6e-14
Identity = 57/171 (33.33%), Postives = 76/171 (44.44%), Query Frame = 1


Query: 15  LCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVG 74
           L   +AI  +     PPV DTEG  L  D  Y++ PA     G LT+  R    CP  V 
Sbjct: 10  LLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRVLP-CPLLVA 69

Query: 75  QVPLLSQEIGFGVTLTPY----LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKS 134
           Q     +  GF V  TP+       D TIR   D++  F A TIC+  T+W VG+E P +
Sbjct: 70  Q-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDE-PLT 129

Query: 135 GRRFVKLG-----NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGS 177
           G R V  G     + +     FR++K   G Y +  C       G  R G+
Sbjct: 130 GARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

[Close]

BLAST of CSPI02G03680 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 47/166 (28.31%), Postives = 75/166 (45.18%), Query Frame = 1


Query: 8   LIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDV-AGNLTLITRKG 67
           L+  F+   +F  + + A     PVLDT+G  LQ  V+Y++  +I+    G L L    G
Sbjct: 10  LLFAFTSKSYFFGVANAAN---SPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATG 69

Query: 68  DQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRD--LKFVFQAFTICITGTQWKVG 127
             CP  V Q      + G  V  +     DD +R   D  ++FV     +C T T W++ 
Sbjct: 70  QSCPEIVVQ-RRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 129

Query: 128 EEDPKSGRRFVKLG------NDNTATGYFRIDKSNLGVYNIGWCPS 165
             D  +G+ +V           NT   +F+I+K+ +  Y   +CPS
Sbjct: 130 NYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]

BLAST of CSPI02G03680 vs. Swiss-Prot
Match: KTI1_SOYBN (Kunitz-type trypsin inhibitor KTI1 OS=Glycine max GN=KTI1 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-08
Identity = 58/190 (30.53%), Postives = 86/190 (45.26%), Query Frame = 1


Query: 10  ALFSWLCFFMA-IPS-TAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGD 69
           ALF    F ++ +PS TAQF    VLDT+  PLQ    Y++ P +    G + + +   +
Sbjct: 8   ALFLVCAFTISYLPSATAQF----VLDTDDDPLQNGGTYYMLPVMRGKGGGIEVDSTGKE 67

Query: 70  QCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAF---TICI-TGTQWKV 129
            CP  V Q P    + G G+  T  L     I E   L   F +F   T+C    T+W +
Sbjct: 68  ICPLTVVQSP-NELDKGIGLVFTSPLHA-LFIAERYPLSIKFGSFAVITLCAGMPTEWAI 127

Query: 130 GEEDPKSGRRFVKLGNDNTATGYFRIDK--SNLGVYNIGWCPSDVPFKGRPRCGSAGILI 189
            E   + G + VKL   +T  G+F I++       Y + +CP         +C   GI I
Sbjct: 128 VE---REGLQAVKLAARDTVDGWFNIERVSREYNDYKLVFCPQQAEDN---KCEDIGIQI 185

Query: 190 -EKGVRFLAL 191
            + G+R L L
Sbjct: 188 DDDGIRRLVL 185

[Close]

BLAST of CSPI02G03680 vs. Swiss-Prot
Match: IELK1_BAURF (Kunitz-type elastase inhibitor BrEI OS=Bauhinia rufa PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.8e-08
Identity = 43/141 (30.50%), Postives = 56/141 (39.72%), Query Frame = 1


Query: 31  PVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL--LSQEIGFGVT 90
           PVLD  G PL    +Y++ P I    G L+        CP  V Q+P   L Q  G  + 
Sbjct: 3   PVLDANGDPLVPGGQYYVLPHIWPGPGGLSFEKTGNQTCPVSVFQLPRLPLEQNNGKPLV 62

Query: 91  LTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGND------NT 150
            TP  E DD I E   ++  F     C    +W +   D K     V +G         T
Sbjct: 63  FTPVSETDD-INEDTAVEIAFAEPPSCAESGKWLI-VNDFKEEYWSVGIGGPQDHEGYQT 122

Query: 151 ATGYFRIDKSNLGVYNIGWCP 164
            TGYF+I K     Y   + P
Sbjct: 123 LTGYFKIHKVGSFAYMFSFLP 141

[Close]

BLAST of CSPI02G03680 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.3e-126
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 1


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of CSPI02G03680 vs. TrEMBL
Match: D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 7.8e-55
Identity = 116/195 (59.49%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 12  FSWLCFFMAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 71
           F WL   MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +
Sbjct: 10  FVWLT--MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIR 69

Query: 72  CPFYVGQVPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEED 131
           CPFYVGQ PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEED
Sbjct: 70  CPFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEED 129

Query: 132 PKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVR 191
           P++GRRF+    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R
Sbjct: 130 PETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKR 189

Query: 192 FLALDGPAFPFEFVR 202
            LALDGPAFPF F R
Sbjct: 190 LLALDGPAFPFVFTR 198

[Close]

BLAST of CSPI02G03680 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 7.8e-55
Identity = 116/195 (59.49%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 12  FSWLCFFMAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 71
           F WL   MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +
Sbjct: 10  FVWLT--MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIR 69

Query: 72  CPFYVGQVPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEED 131
           CPFYVGQ PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEED
Sbjct: 70  CPFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEED 129

Query: 132 PKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVR 191
           P++GRRF+    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R
Sbjct: 130 PETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKR 189

Query: 192 FLALDGPAFPFEFVR 202
            LALDGPAFPF F R
Sbjct: 190 LLALDGPAFPFVFTR 198

[Close]

BLAST of CSPI02G03680 vs. TrEMBL
Match: G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.0e-54
Identity = 115/195 (58.97%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 12  FSWLCFFMAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 71
           F WL   MAI S AQ      PPVLDT G+PL+  VEY+I P ITD+AG LTL+ R G +
Sbjct: 10  FVWLT--MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIR 69

Query: 72  CPFYVGQVPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEED 131
           CPFYVGQ PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEED
Sbjct: 70  CPFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEED 129

Query: 132 PKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVR 191
           P++GRRF+    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R
Sbjct: 130 PETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKR 189

Query: 192 FLALDGPAFPFEFVR 202
            LALDGPAFPF F R
Sbjct: 190 LLALDGPAFPFVFTR 198

[Close]

BLAST of CSPI02G03680 vs. TrEMBL
Match: E9NH21_POPNI (Kunitz-type trypsin inhibitor OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-54
Identity = 116/195 (59.49%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 12  FSWLCFFMAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 71
           F WL   MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +
Sbjct: 10  FVWLT--MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIR 69

Query: 72  CPFYVGQVPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEED 131
           CPFYVGQ PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEED
Sbjct: 70  CPFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEED 129

Query: 132 PKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVR 191
           P++GRRF+    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R
Sbjct: 130 PETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKR 189

Query: 192 FLALDGPAFPFEFVR 202
            LALDGPAFPF F R
Sbjct: 190 LLALDGPAFPFVFTR 198

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 460.3 bits (1183), Expect = 1.9e-126
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 1


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 412.5 bits (1059), Expect = 4.5e-112
Identity = 195/216 (90.28%), Postives = 202/216 (93.52%), Query Frame = 1


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 216
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 404.8 bits (1039), Expect = 9.3e-110
Identity = 194/216 (89.81%), Postives = 200/216 (92.59%), Query Frame = 1


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM  HKSL A+FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMMLHKSL-AIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 216
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 215

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BLAST of CSPI02G03680 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-57
Identity = 114/201 (56.72%), Postives = 136/201 (67.66%), Query Frame = 1


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM  HKSL A+F + C FMAI STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLT
Sbjct: 1   MMMLHKSL-AIFGYACLFMAITSTAQL--PPVLDTNGQPLQRGVEYYISPAITDVGGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           L +R    CP +VGQ P+ S  IG  VT  P  EG D I EG  L  VFQA + C+T TQ
Sbjct: 61  LKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           W+V   +  +GRRFV +G+++   G F I + N G YNI WCP+     GRPRCG AGIL
Sbjct: 121 WRVDATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVR 202
           +E GVR +ALDG AFPFEF++
Sbjct: 181 VENGVRLVALDGDAFPFEFIK 194

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BLAST of CSPI02G03680 vs. NCBI nr
Match: gi|242346705|gb|ACS92521.1| (Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides])

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-54
Identity = 116/195 (59.49%), Postives = 130/195 (66.67%), Query Frame = 1


Query: 12  FSWLCFFMAIPSTAQ---FDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 71
           F WL   MAI S AQ      PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +
Sbjct: 10  FVWLT--MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIR 69

Query: 72  CPFYVGQVPLLSQE-IGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEED 131
           CPFYVGQ PL S E  G  V  TPY  G+  IRE RDL   FQA TICI  T W+VGEED
Sbjct: 70  CPFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEED 129

Query: 132 PKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSD-VPFKGRPRCGSAGILIEKGVR 191
           P++GRRF+    D +   YFRID +N GVYN  WCP++  P   RPRCGSAGILIE   R
Sbjct: 130 PETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKR 189

Query: 192 FLALDGPAFPFEFVR 202
            LALDGPAFPF F R
Sbjct: 190 LLALDGPAFPFVFTR 198

The following BLAST results are available for this feature:

BLAST of CSPI02G03680 vs. Swiss-Prot


Match Name	E-value	Identity	Description
IAAS_WHEAT	4.3e-14	35.11	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1	[more]
IAAS_ORYSJ	9.6e-14	33.33	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
ASP_THECC	2.9e-10	28.31	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]
KTI1_SOYBN	1.6e-08	30.53	Kunitz-type trypsin inhibitor KTI1 OS=Glycine max GN=KTI1 PE=2 SV=1	[more]
IELK1_BAURF	7.8e-08	30.50	Kunitz-type elastase inhibitor BrEI OS=Bauhinia rufa PE=1 SV=1	[more]

BLAST of CSPI02G03680 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4_CUCSA	1.3e-126	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1	[more]
D1KFM4_9ROSI	7.8e-55	59.49	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=...	[more]
D1KFM3_9ROSI	7.8e-55	59.49	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides...	[more]
G0Z2L7_POPNI	1.0e-54	58.97	Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1	[more]
E9NH21_POPNI	1.3e-54	59.49	Kunitz-type trypsin inhibitor OS=Populus nigra PE=2 SV=1	[more]

BLAST of CSPI02G03680 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CSPI02G03680 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442849\|ref\|XP_004139193.1\|	1.9e-126	100.00	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus]	[more]
gi\|659070426\|ref\|XP_008455014.1\|	4.5e-112	90.28	PREDICTED: miraculin-like isoform X1 [Cucumis melo]	[more]
gi\|659070428\|ref\|XP_008455022.1\|	9.3e-110	89.81	PREDICTED: miraculin-like isoform X2 [Cucumis melo]	[more]
gi\|449442847\|ref\|XP_004139192.1\|	2.4e-57	56.72	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|242346705\|gb\|ACS92521.1\|	1.1e-54	59.49	Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G03680.1	CSPI02G03680.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..200 score: 1.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..203 score: 8.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 29..203 score: 3.55
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 30..203 score: 1.3
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 5..207 score: 7.4
None	No IPR available	PANTHER	PTHR33107:SF4	SUBFAMILY NOT NAMED	coord: 5..207 score: 7.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G03680	Cla019870	Watermelon (97103) v1	cpiwmB149
CSPI02G03680	Csa2G021000	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G03680	MELO3C004296	Melon (DHL92) v3.5.1	cpimeB139
CSPI02G03680	ClCG02G015990	Watermelon (Charleston Gray)	cpiwcgB128
CSPI02G03680	Lsi11G005210	Bottle gourd (USVL1VR-Ls)	cpilsiB096
CSPI02G03680	MELO3C000887.2	Melon (DHL92) v3.6.1	cpimedB132
CSPI02G03680	CsaV3_2G005010	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G03680	Cla97C02G041760	Watermelon (97103) v2	cpiwmbB120
CSPI02G03680	Bhi05G001663	Wax gourd	cpiwgoB149
CSPI02G03680	Cucsa.091720	Cucumber (Gy14) v1	cgycpiB097
CSPI02G03680	CmaCh05G011550	Cucurbita maxima (Rimu)	cmacpiB800
CSPI02G03680	CmaCh12G010860	Cucurbita maxima (Rimu)	cmacpiB174
CSPI02G03680	CmoCh12G011020	Cucurbita moschata (Rifu)	cmocpiB159
CSPI02G03680	Cp4.1LG07g09060	Cucurbita pepo (Zucchini)	cpecpiB821
CSPI02G03680	Cp4.1LG11g09390	Cucurbita pepo (Zucchini)	cpecpiB100
CSPI02G03680	CsGy2G003790	Cucumber (Gy14) v2	cgybcpiB056
CSPI02G03680	Carg12887	Silver-seed gourd	carcpiB0938
CSPI02G03680	Carg17559	Silver-seed gourd	carcpiB0751

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G03680	Cucurbita moschata (Rifu)	cmocpiB787