Carg17559 (gene) Silver-seed gourd

Overview
NameCarg17559
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionKunitz-type trypsin inhibitor
LocationCucurbita_argyrosperma_scaffold_183 : 235133 .. 235815 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTATTATTACATCACAATCGAACTCAAAAACTTTTGATTAGTGATACCATTAGAAAACTCTTGATTATGTTACGAATTCTTAACCATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

mRNA sequence

ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Coding sequence (CDS)

ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFVRA
Homology
[Close]
BLAST of Carg17559 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 410.2 bits (1053), Expect = 3.9e-111
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0 

Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]
BLAST of Carg17559 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 2.5e-110
Identity = 194/197 (98.48%), Postives = 197/197 (100.00%), Query Frame = 0 

Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGD+DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 TDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]
BLAST of Carg17559 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-107
Identity = 190/197 (96.45%), Postives = 192/197 (97.46%), Query Frame = 0 

Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMA SSIAQSPPVLD EGRPL+INVEYFIKPA+TDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLP ED IREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
            DPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 ADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]
BLAST of Carg17559 vs. NCBI nr
Match: XP_022150807.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 332.0 bits (850), Expect = 1.4e-87
Identity = 159/197 (80.71%), Postives = 174/197 (88.32%), Query Frame = 0 

Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           +L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTRK
Sbjct: 4   LLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRK 63

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GD CPFHVGQVPLRS +IG+ +    +L GEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 64  GDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWRVGE 123

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           T+PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG+
Sbjct: 124 TEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGV 183

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 184 RLLALDGSAFPFEFVRA 200

[Close]
BLAST of Carg17559 vs. NCBI nr
Match: XP_004139193.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] >KGN60878.1 hypothetical protein Csa_2G021000 [Cucumis sativus])

HSP 1 Score: 327.4 bits (838), Expect = 3.3e-86
Identity = 155/196 (79.08%), Postives = 172/196 (87.76%), Query Frame = 0 

Query: 4   KSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 63
           KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRK
Sbjct: 6   KSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRK 65

Query: 64  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 123
           GDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE
Sbjct: 66  GDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGE 125

Query: 124 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 183
            DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+
Sbjct: 126 EDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGV 185

Query: 184 RLLALDGSAFPFEFVR 197
           R LALDG AFPFEFVR
Sbjct: 186 RFLALDGPAFPFEFVR 201

[Close]
BLAST of Carg17559 vs. TAIR10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-16
Identity = 59/195 (30.26%), Postives = 90/195 (46.15%), Query Frame = 0 

Query: 14  LVMAVSSIAQS---PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQ 73
           LV+A++++  S     V+D +G  +  +  Y++ P +    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 74  VPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRF 133
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 134 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 193
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 194 LALDGSAFPFEFVRA 198
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]
BLAST of Carg17559 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 48.5 bits (114), Expect = 5.4e-06
Identity = 43/165 (26.06%), Postives = 66/165 (40.00%), Query Frame = 0 

Query: 2   LCKSLVAFSYLCLVMAVSSIAQSPP---VLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 61
           L  S +  + L  +   +S A + P   VLD  G P+Q NV+Y+I PA     G L    
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 62  RK---GDQCPFHVGQVPLRSQEI-GFGVTFTPYLPGEDTIREGRDLKVVFQA-FSICITS 121
           R     D C  ++  V   S  + G  VTF+P       ++    L + F +   +C  S
Sbjct: 64  RNLSTQDLC-LNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDS 123

Query: 122 TAWRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCP 159
             WR+  +           G       +F+I  D    Y + +CP
Sbjct: 124 KVWRIDHSVQLRKSFVSIGGQKGKGNSWFQIQEDG-DAYKLMYCP 166

[Close]
BLAST of Carg17559 vs. Swiss-Prot
Match: sp|Q8RXD5|KTI1_ARATH (Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.6e-15
Identity = 59/195 (30.26%), Postives = 90/195 (46.15%), Query Frame = 0 

Query: 14  LVMAVSSIAQS---PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQ 73
           LV+A++++  S     V+D +G  +  +  Y++ P +    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 74  VPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRF 133
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 134 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 193
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 194 LALDGSAFPFEFVRA 198
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]
BLAST of Carg17559 vs. Swiss-Prot
Match: sp|P07596|IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 48/135 (35.56%), Postives = 70/135 (51.85%), Query Frame = 0 

Query: 19  SSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEI 78
           S  A  PPV DT+G  L+ +  Y++  A     G LT+    G  CP  V Q P   Q  
Sbjct: 20  SRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHD 79

Query: 79  GFGVTFTPY--LPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG--D 138
           GF V  TPY   P +  IR   D+++ F+A++ C+ ST W + +++   GRR V TG   
Sbjct: 80  GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHVITGPVK 139

Query: 139 DDTATG---YFRIDR 147
           D + +G    FRI++
Sbjct: 140 DPSPSGRENAFRIEK 152

[Close]
BLAST of Carg17559 vs. Swiss-Prot
Match: sp|P29421|IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 4.0e-14
Identity = 52/137 (37.96%), Postives = 70/137 (51.09%), Query Frame = 0 

Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           ++  SL+A S+ C        A  PPV DTEG  L  +  Y++ PA     G LT+  R 
Sbjct: 8   LILLSLLAISFSC-------SAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 67

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPY----LPGEDTIREGRDLKVVFQAFSICITSTAW 120
              CP  V Q     +  GF V FTP+     P + TIR   D+++ F A +IC+ ST W
Sbjct: 68  -LPCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 127

Query: 121 RVGETDPTTGRRFVETG 134
            VG+ +P TG R V TG
Sbjct: 128 HVGD-EPLTGARRVVTG 134

[Close]
BLAST of Carg17559 vs. Swiss-Prot
Match: sp|P16347|IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.7e-12
Identity = 43/129 (33.33%), Postives = 68/129 (52.71%), Query Frame = 0 

Query: 25  PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTF 84
           PPV DT+G  L+ +  Y++ PA     G LT+    G +CP  V Q     Q  G  V  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQ-EADGQRDGLPVRI 62

Query: 85  TPY--LPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD--DDTATG 144
            P+   P +  IR   D+++ F+A++ C+ ST W + +++  +GRR V TG   D + +G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSPSG 122

Query: 145 ---YFRIDR 147
               FRI++
Sbjct: 123 RENAFRIEK 129

[Close]
BLAST of Carg17559 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.5e-10
Identity = 50/164 (30.49%), Postives = 77/164 (46.95%), Query Frame = 0 

Query: 6   LVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDV-AGNLTLLTRKGDQC 65
           L AF+       V++ A S PVLDT+G  LQ  V+Y++  +++    G L L    G  C
Sbjct: 11  LFAFTSKSYFFGVANAANS-PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 70

Query: 66  PFHVGQVPLRSQ-EIGFGVTFTPYLPGEDTIREGRDLKVVFQAF--SICITSTAWRVGET 125
           P  V  V  RS  + G  V F+     +D +R   D+ + F      +C TST WR+   
Sbjct: 71  PEIV--VQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNY 130

Query: 126 DPTTGRRFVETG------DDDTATGYFRIDRDNFGVYNIGWCPS 160
           D + G+ +V T         +T   +F+I++     Y   +CPS
Sbjct: 131 DNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]
BLAST of Carg17559 vs. TrEMBL
Match: tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.2e-86
Identity = 155/196 (79.08%), Postives = 172/196 (87.76%), Query Frame = 0 

Query: 4   KSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 63
           KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRK
Sbjct: 6   KSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRK 65

Query: 64  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 123
           GDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE
Sbjct: 66  GDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGE 125

Query: 124 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 183
            DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+
Sbjct: 126 EDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGV 185

Query: 184 RLLALDGSAFPFEFVR 197
           R LALDG AFPFEFVR
Sbjct: 186 RFLALDGPAFPFEFVR 201

[Close]
BLAST of Carg17559 vs. TrEMBL
Match: tr|A0A1S3C0M7|A0A1S3C0M7_CUCME (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.2e-81
Identity = 148/197 (75.13%), Postives = 163/197 (82.74%), Query Frame = 0 

Query: 4   KSLVA-FSYLCLVMAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 63
           KSL + FS LC  MA+ S A     PPVLDTEGRPLQ + EYFIKPA+TDVAGNLTL+TR
Sbjct: 6   KSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITR 65

Query: 64  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 123
           KGDQCPF+VGQVPL SQE GF V+  PY  GEDTIREGRDLK VFQ F+ICIT T W+VG
Sbjct: 66  KGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVG 125

Query: 124 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 183
           E DP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGILIEKG
Sbjct: 126 EADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKG 185

Query: 184 LRLLALDGSAFPFEFVR 197
           +R LALDG AFPFEFVR
Sbjct: 186 VRFLALDGPAFPFEFVR 202

[Close]
BLAST of Carg17559 vs. TrEMBL
Match: tr|D1KFM3|D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides OX=3695 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.3e-56
Identity = 119/195 (61.03%), Postives = 136/195 (69.74%), Query Frame = 0 

Query: 10  SYLCLVMAVSSIA-----QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQC 69
           S++ L MA+ S+A     QSPPVLDT G+PL+  VEY+I P +TDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 70  PFHVGQVPLRSQE-IGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDP 129
           PF+VGQ PL S E  G  V FTPY  GE  IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 130 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 189
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 190 LALDGSAFPFEFVRA 198
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]
BLAST of Carg17559 vs. TrEMBL
Match: tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.3e-56
Identity = 110/182 (60.44%), Postives = 128/182 (70.33%), Query Frame = 0 

Query: 16  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 75
           MA++S AQ PPVLDT G+PLQ  VEY+I PA+TDV GNLTL +R    CP  VGQ P+ S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 76  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 135
             IG  VTF P   G D I EG  L +VFQA S C+TST WRV  T+  TGRRFV  GD+
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 136 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 195
           D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G+RL+ALDG AFPFEF+
Sbjct: 121 DGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFEFI 178

Query: 196 RA 198
           +A
Sbjct: 181 KA 178

[Close]
BLAST of Carg17559 vs. TrEMBL
Match: tr|G0Z2L7|G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.3e-56
Identity = 118/195 (60.51%), Postives = 136/195 (69.74%), Query Frame = 0 

Query: 10  SYLCLVMAVSSIA-----QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQC 69
           S++ L MA+ S+A     QSPPVLDT G+PL+  VEY+I P +TD+AG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRC 68

Query: 70  PFHVGQVPLRSQE-IGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDP 129
           PF+VGQ PL S E  G  V FTPY  GE  IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 130 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 189
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRL 188

Query: 190 LALDGSAFPFEFVRA 198
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

The following BLAST results are available for this feature:
BLAST of Carg17559 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022938134.13.9e-111100.00alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata][more]
XP_023521354.12.5e-11098.48alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381... [more]
XP_022965952.11.5e-10796.45kunitz trypsin inhibitor 1-like [Cucurbita maxima][more]
XP_022150807.11.4e-8780.71miraculin-like [Momordica charantia][more]
XP_004139193.13.3e-8679.08PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] ... [more]
BLAST of Carg17559 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73260.12.0e-1630.26kunitz trypsin inhibitor 1[more]
AT1G73325.15.4e-0626.06Kunitz family trypsin and protease inhibitor protein[more]
BLAST of Carg17559 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8RXD5|KTI1_ARATH3.6e-1530.26Kunitz trypsin inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=KTI1 PE=2 SV=1[more]
sp|P07596|IAAS_HORVU1.4e-1435.56Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2[more]
sp|P29421|IAAS_ORYSJ4.0e-1437.96Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R... [more]
sp|P16347|IAAS_WHEAT1.7e-1233.33Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ... [more]
sp|P32765|ASP_THECC4.5e-1030.4921 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1[more]
BLAST of Carg17559 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA2.2e-8679.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1[more]
tr|A0A1S3C0M7|A0A1S3C0M7_CUCME6.2e-8175.13endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|D1KFM3|D1KFM3_9ROSI5.3e-5661.03Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA5.3e-5660.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1[more]
tr|G0Z2L7|G0Z2L7_POPNI5.3e-5660.51Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg17559-RACarg17559-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 26..197
e-value: 8.9E-22
score: 88.3
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 27..195
e-value: 1.0E-36
score: 126.4
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107FAMILY NOT NAMEDcoord: 7..196
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 27..43
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 23..197
e-value: 3.0E-39
score: 136.5
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 7..196
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILYSSF50386STI-likecoord: 25..196