Cp4.1LG11g09390 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG11g09390
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionKunitz-type trypsin inhibitor
LocationCp4.1LG11 : 8022995 .. 8023576 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

mRNA sequence

ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Coding sequence (CDS)

ATGTTGCATAATTCACTTGCCATCTTTAGCTACCTATTAGTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCAGTGCTCGACACCGACGGTCAACCTCTACAACGTGGTGTTGAGTACTACATCAAGCCTGCAATCACCGACGTTGCAGGTAATCTCACCCTAAAAAGTCGTAGCAATGCTCCTTGCCCGCTCTACGTCGGTCAAGAACCAGTTGCTTCAACAAATATCGGTCTCCCAGTCACCTTCACTCCCATCGTGGCGGGCGAAGATATAATTGAAGAAAGTATGGGTTTCAATGTTGCGTTTCAAGCATCGTCGACGTGCATAACATCGACACAATGGAGAGTGGATGAGATGGAGTCTGCAACAGGGAGGAGGTTCGTGGGGGTCGGGAACGATAACAGTCCGACTGGGATCTTTAGGATCGACAGGAACAATGGTGTTTACAATATAGTATGGTGCCCTGAGATGGTAGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTGGAGGATGGAGTGAGGCTGCTGGCTTTGGATGGCAATGCGTTTCCTTTTGAGTTTGTCAAAGCTTGA

Protein sequence

MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA
Homology
[Close]
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 96.7 bits (239), Expect = 3.0e-19
Identity = 63/183 (34.43%), Postives = 90/183 (49.18%), Query Frame = 1 

Query: 26  PVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKS---RSNAPCPLYVGQEPVASTNIGLPV 85
           PVLD DG+ L+ G  YYI P + D  G LT+ +        CP  V Q      +   P+
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR-KEVDHDRPL 94

Query: 86  TFTPIVAGEDIIEESMGFNVAFQASSTC--ITSTQWRVDEMESATGRRFVGVGNDNSPTG 145
            F P    ED++  S   N+ F A   C   +ST WR+D+ + +TG+ FV +G      G
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 146 ------IFRIDR--NNGVYNIVWCPEMVG--RPRCGSAGILVED-GVRLLALDGNAFPFE 193
                  F+I+    +G Y +V+CP + G  + +CG  GI ++  G R LAL    F FE
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFE 214

[Close]
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 7.0e-16
Identity = 55/153 (35.95%), Postives = 78/153 (50.98%), Query Frame = 1 

Query: 22  AQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVASTNIGLP 81
           A  PPV DTDG  L+    YY+  A     G LT+       CPL+V Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDPNGQHD-GFP 82

Query: 82  VTFTP--IVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVG--NDNS 141
           V  TP  +   + II  S    ++F+A +TC+ ST+W +D  E A GRR V  G   D S
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDS-ELAAGRRHVITGPVKDPS 142

Query: 142 PTG---IFRIDRNNGV----YNIV----WCPEM 160
           P+G    FRI++ +G     Y ++    WC ++
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSCGDWCQDL 173

[Close]
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.7e-15
Identity = 53/143 (37.06%), Postives = 73/143 (51.05%), Query Frame = 1 

Query: 25  PPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVASTNIGLPVTF 84
           PPV DTDG  L+    YY+ PA     G LT+       CPL+V QE     + GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRD-GLPVRI 62

Query: 85  TPI--VAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVG--NDNSPTG 144
            P      + II  S    ++F+A +TC+ ST+W +D  E  +GRR V  G   D SP+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDS-ELVSGRRHVITGPVRDPSPSG 122

Query: 145 ---IFRIDRNNGV----YNIVWC 157
               FRI++ +G     Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

[Close]
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 65/186 (34.95%), Postives = 87/186 (46.77%), Query Frame = 1 

Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           M+   L +    L+ ++ + +A  PPV DT+G  L     YY+ PA     G LT+  R 
Sbjct: 1   MVSLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 60

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTP---IVAGED-IIEESMGFNVAFQASSTCITSTQW 120
             PCPL V QE       G PV FTP     A ED  I  S    + F A++ C+ ST+W
Sbjct: 61  -LPCPLLVAQE-TDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 120

Query: 121 RVDEMESATGRRFVGVGN--DNSPTG---IFRIDRNNGVYNIVWCPEMVGRPRCGSAGIL 178
            V + E  TG R V  G     SP+G    FR+++  G Y +V C     R  C   G+ 
Sbjct: 121 HVGD-EPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSC-----RDSCQDLGV- 177

[Close]
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.4e-13
Identity = 63/206 (30.58%), Postives = 94/206 (45.63%), Query Frame = 1 

Query: 6   LAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDV-AGNLTLKSRSNAPC 65
           L  F+    F  + + A   PVLDTDG  LQ GV+YY+  +I+    G L L   +   C
Sbjct: 11  LFAFTSKSYFFGVANAAN-SPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 70

Query: 66  PLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQ--ASSTCITSTQWRVDEME 125
           P  V Q      N G PV F+   + +D++  S   N+ F       C TST WR+D  +
Sbjct: 71  PEIVVQRRSDLDN-GTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYD 130

Query: 126 SATGRRFV---GVGNDNSPTGI---FRIDRNNGV-YNIVWCPEMVGRPRCGSAGILV--- 185
           ++ G+ +V   GV  +  P  +   F+I++   + Y   +CP +     C S   L    
Sbjct: 131 NSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSV-----CDSCTTLCSDI 190

Query: 186 -----EDGVRLLALDGNAFPFEFVKA 194
                +DG   LAL  N + + F KA
Sbjct: 191 GRHSDDDGQIRLALSDNEWAWMFKKA 209

[Close]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match: A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.3e-77
Identity = 139/178 (78.09%), Postives = 154/178 (86.52%), Query Frame = 1 

Query: 16  MAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVAS 75
           MAITSTAQLPPVLDT+GQPLQRGVEYYI PAITDV GNLTLKSRSNAPCPL+VGQEPV S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135
           TNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD  ES TGRRFVG+G++
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 136 NSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 194
           + P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 6.5e-61
Identity = 125/197 (63.45%), Postives = 144/197 (73.10%), Query Frame = 1 

Query: 3   HNSL-AIFSYLLVFMAITSTAQL--PPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSR 62
           H SL A+FS+L  FMAI STAQ   PPVLDT+G+PLQR VEY+IKPAITDVAGNLTL +R
Sbjct: 5   HKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITR 64

Query: 63  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 122
               CP YVGQ P+ S  IG  VT TP + G+D I E       FQA + CIT TQW+V 
Sbjct: 65  KGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVG 124

Query: 123 EMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVEDG 182
           E +  +GRRFV +GNDN+ TG FRID++N GVYNI WCP  V   GRPRCGSAGIL+E G
Sbjct: 125 EEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKG 184

Query: 183 VRLLALDGNAFPFEFVK 193
           VR LALDG AFPFEFV+
Sbjct: 185 VRFLALDGPAFPFEFVR 201

[Close]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match: B9N8J2_POPTR (Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPTR_0004s00220g PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.9e-50
Identity = 112/194 (57.73%), Postives = 126/194 (64.95%), Query Frame = 1 

Query: 10  SYLLVFMAITSTAQ-----LPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 69
           S++ + MAI S AQ      PPVLDT GQPL+ GVEYYI P ITDVAG LTL +R+   C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 70  PLYVGQEPVASTN-IGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMES 129
           P YVGQEP+AS    G  V FTP  +GE II ES   +V FQAS+ CI ST WRV E + 
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDP 128

Query: 130 ATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCP----EMVGRPRCGSAGILVEDGVRLL 189
            TGRRF+    D S    FRID N GVYN  WCP        RPRCGSAGIL+ED  RLL
Sbjct: 129 ETGRRFIVTAGDKS---YFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLL 188

Query: 190 ALDGNAFPFEFVKA 194
           ALDG AFPF F +A
Sbjct: 189 ALDGPAFPFVFTRA 199

[Close]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match: E9NH21_POPNI (Kunitz-type trypsin inhibitor OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.9e-50
Identity = 112/194 (57.73%), Postives = 126/194 (64.95%), Query Frame = 1 

Query: 10  SYLLVFMAITSTAQ-----LPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 69
           S++ + MAI S AQ      PPVLDT GQPL+ GVEYYI P ITDVAG LTL +R+   C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 70  PLYVGQEPVASTN-IGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMES 129
           P YVGQEP+AS    G  V FTP  +GE II ES   +V FQAS+ CI ST WRV E + 
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDP 128

Query: 130 ATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCP----EMVGRPRCGSAGILVEDGVRLL 189
            TGRRF+    D S    FRID N GVYN  WCP        RPRCGSAGIL+ED  RLL
Sbjct: 129 ETGRRFIVTAGDKS---YFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLL 188

Query: 190 ALDGNAFPFEFVKA 194
           ALDG AFPF F +A
Sbjct: 189 ALDGPAFPFVFTRA 199

[Close]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 111/194 (57.22%), Postives = 125/194 (64.43%), Query Frame = 1 

Query: 10  SYLLVFMAITSTAQ-----LPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPC 69
           S++ + MAI S AQ      PPVLDT GQPL+ GVEYYI P ITDVAG LTL +R+   C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 70  PLYVGQEPVASTN-IGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMES 129
           P YVGQEP+AS    G  V FTP  +GE II ES   +V FQA + CI ST WRV E + 
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 130 ATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCP----EMVGRPRCGSAGILVEDGVRLL 189
            TGRRF+    D S    FRID N GVYN  WCP        RPRCGSAGIL+ED  RLL
Sbjct: 129 ETGRRFIVTAGDKS---YFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLL 188

Query: 190 ALDGNAFPFEFVKA 194
           ALDG AFPF F +A
Sbjct: 189 ALDGPAFPFVFTRA 199

[Close]
BLAST of Cp4.1LG11g09390 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 77.8 bits (190), Expect = 8.2e-15
Identity = 62/199 (31.16%), Postives = 88/199 (44.22%), Query Frame = 1 

Query: 6   LAIFSYLLVFMA---ITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNA 65
           L IF  L VF++   +T+ A + PV D +G+ L  GV YYI P I    G LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 66  PCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEME 125
            CP  V Q+     + GLPV F+P       I  S   N+ F  +S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKSR-TIPVSTDVNIKFSPTSI------WELANFD 124

Query: 126 SATGRRFVGV----GNDNSPT--GIFRIDRNNGVYNIVWCPEMVG--RPRCGSAGILVED 185
             T + F+      GN    T    F+ID+    Y I +CP +    +  C   G+ V+D
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 184

Query: 186 GVRLLALDGNAFPFEFVKA 194
           G R LAL        F +A
Sbjct: 185 GKRRLALSDVPLKVMFKRA 195

[Close]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 315.1 bits (806), Expect = 8.6e-83
Identity = 149/193 (77.20%), Postives = 165/193 (85.49%), Query Frame = 1 

Query: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60
           MLH SLAIF Y  +FMAITSTAQLPPVLDT+GQPLQRGVEYYI PAITDV GNLTLKSRS
Sbjct: 3   MLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62

Query: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120
           NAPCPL+VGQEPV STNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD 
Sbjct: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122

Query: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180
            ES TGRRFVG+G+++ P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182

Query: 181 LDGNAFPFEFVKA 194
           LDG+AFPFEF+KA
Sbjct: 183 LDGDAFPFEFIKA 195

[Close]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: gi|700205760|gb|KGN60879.1| (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 297.4 bits (760), Expect = 1.9e-77
Identity = 139/178 (78.09%), Postives = 154/178 (86.52%), Query Frame = 1 

Query: 16  MAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPVAS 75
           MAITSTAQLPPVLDT+GQPLQRGVEYYI PAITDV GNLTLKSRSNAPCPL+VGQEPV S
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 76  TNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFVGVGND 135
           TNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD  ES TGRRFVG+G++
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 136 NSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 194
           + P GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 241.9 bits (616), Expect = 9.3e-61
Identity = 125/197 (63.45%), Postives = 144/197 (73.10%), Query Frame = 1 

Query: 3   HNSL-AIFSYLLVFMAITSTAQL--PPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSR 62
           H SL A+FS+L  FMAI STAQ   PPVLDT+G+PLQR VEY+IKPAITDVAGNLTL +R
Sbjct: 5   HKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITR 64

Query: 63  SNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVD 122
               CP YVGQ P+ S  IG  VT TP + G+D I E       FQA + CIT TQW+V 
Sbjct: 65  KGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVG 124

Query: 123 EMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVEDG 182
           E +  +GRRFV +GNDN+ TG FRID++N GVYNI WCP  V   GRPRCGSAGIL+E G
Sbjct: 125 EEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKG 184

Query: 183 VRLLALDGNAFPFEFVK 193
           VR LALDG AFPFEFV+
Sbjct: 185 VRFLALDGPAFPFEFVR 201

[Close]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 4.3e-58
Identity = 122/199 (61.31%), Postives = 138/199 (69.35%), Query Frame = 1 

Query: 1   MLHNSLAIFSYLLVFMAITSTAQL---PPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLK 60
           MLH SLAIFS L  FMAI STA     PPVLDT+G+PLQR  EY+IKPAITDVAGNLTL 
Sbjct: 3   MLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLI 62

Query: 61  SRSNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWR 120
           +R    CP YVGQ P+ S   G  V+  P   GED I E       FQ  + CIT TQW+
Sbjct: 63  TRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWK 122

Query: 121 VDEMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVE 180
           V E +  TGRRFV +G DN+ TG FRID+++ GVYNI WCP  V   GRPRCGSAGIL+E
Sbjct: 123 VGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIE 182

Query: 181 DGVRLLALDGNAFPFEFVK 193
            GVR LALDG AFPFEFV+
Sbjct: 183 KGVRFLALDGPAFPFEFVR 201

[Close]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-55
Identity = 119/198 (60.10%), Postives = 136/198 (68.69%), Query Frame = 1 

Query: 3   HNSL-AIFSYLLVFMAITSTAQL---PPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKS 62
           H SL +IFS L  FMAI STA     PPVLDT+G+PLQR  EY+IKPAITDVAGNLTL +
Sbjct: 5   HKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLIT 64

Query: 63  RSNAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRV 122
           R    CP YVGQ P+ S   G  V+  P   GED I E       FQ  + CIT TQW+V
Sbjct: 65  RKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKV 124

Query: 123 DEMESATGRRFVGVGNDNSPTGIFRIDRNN-GVYNIVWCPEMV---GRPRCGSAGILVED 182
            E +  TGRRFV +G DN+ TG FRID+++ GVYNI WCP  V   GRPRCGSAGIL+E 
Sbjct: 125 GEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEK 184

Query: 183 GVRLLALDGNAFPFEFVK 193
           GVR LALDG AFPFEFV+
Sbjct: 185 GVRFLALDGPAFPFEFVR 202

The following BLAST results are available for this feature:
BLAST of Cp4.1LG11g09390 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MIRA_SYNDU3.0e-1934.43Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
IAAS_HORVU7.0e-1635.95Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2[more]
IAAS_WHEAT7.7e-1537.06Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1[more]
IAAS_ORYSJ1.3e-1434.95Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ... [more]
ASP_THECC9.4e-1330.5821 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
BLAST of Cp4.1LG11g09390 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LIK2_CUCSA1.3e-7778.09Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1[more]
A0A0A0LGB4_CUCSA6.5e-6163.45Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1[more]
B9N8J2_POPTR3.9e-5057.73Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPT... [more]
E9NH21_POPNI3.9e-5057.73Kunitz-type trypsin inhibitor OS=Populus nigra PE=2 SV=1[more]
D1KFM3_9ROSI2.0e-4957.22Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
BLAST of Cp4.1LG11g09390 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G17860.18.2e-1531.16 Kunitz family trypsin and protease inhibitor protein[more]
BLAST of Cp4.1LG11g09390 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442847|ref|XP_004139192.1|8.6e-8377.20PREDICTED: miraculin-like [Cucumis sativus][more]
gi|700205760|gb|KGN60879.1|1.9e-7778.09hypothetical protein Csa_2G021500 [Cucumis sativus][more]
gi|449442849|ref|XP_004139193.1|9.3e-6163.45PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus][more]
gi|659070428|ref|XP_008455022.1|4.3e-5861.31PREDICTED: miraculin-like isoform X2 [Cucumis melo][more]
gi|659070426|ref|XP_008455014.1|1.2e-5560.10PREDICTED: miraculin-like isoform X1 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g09390.1Cp4.1LG11g09390.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 27..192
score: 4.0
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 26..193
score: 1.5
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 27..43
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 25..193
score: 4.3
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 24..193
score: 8.5
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..193
score: 1.9
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..193
score: 1.9